Abstract: Disclosed is a tumor vaccine based on tumor-derived microparticles (TMPs), and a preparation method and use thereof. The preparation method includes the following steps: 1, subjecting tumor cells to separation to obtain TMPs; 2, mixing the TMPs with 0.03 ?g/?l of a polyethyleneimine solution to allow a reaction for 10 min to 20 min, and washing to obtain PEI-modified TMPs (TMPs-PEI); and 3, mixing the TMP-PEI with 0.1 ?g/?l of a lipopolysaccharide solution to allow cross-linking for 15 min to 1 h to obtain the tumor vaccine based on TMPs (TMP-PEI-LPS). A nanomaterial prepared by conducting surface modification on the TMPs with an immunologic adjuvant can be disguised as a bacterial analog to promote the recognition of tumor antigens in vivo. Moreover, the TMPs can retain their immunostimulatory ability and reduce their toxicity by using a cross-linking agent coupled with the immunologic adjuvant.

Abstract: Multiple geographic grids are generated according to acquired location information of a service provider. Each geographic grid contains one or more service providers. The second region matching the target location information is a geographic grid for a region defined by the target location information. A presentation request for a search page is received. The presentation request includes target location information. A keyword is acquired that has a search popularity meeting a first condition in a first region matching the target location information, and the acquired keyword is used as a first keyword. The first keyword is provided for presentation in the search page.

Abstract: Multiple geographic grids are generated according to acquired location information of a service provider. Each geographic grid contains one or more service providers. The second region matching the target location information is a geographic grid for a region defined by the target location information. A presentation request for a search page is received. The presentation request includes target location information. A keyword is acquired that has a search popularity meeting a first condition in a first region matching the target location information, and the acquired keyword is used as a first keyword. The first keyword is provided for presentation in the search page.

Abstract: Multiple geographic grids are generated according to acquired location information of a service provider. Each geographic grid contains one or more service providers. The second region matching the target location information is a geographic grid for a region defined by the target location information. A presentation request for a search page is received. The presentation request includes target location information. A keyword is acquired that has a search popularity meeting a first condition in a first region matching the target location information, and the acquired keyword is used as a first keyword. The first keyword is provided for presentation in the search page.

Abstract: Multiple geographic grids are generated according to acquired location information of a service provider. Each geographic grid contains one or more service providers. The second region matching the target location information is a geographic grid for a region defined by the target location information. A presentation request for a search page is received. The presentation request includes target location information. A keyword is acquired that has a search popularity meeting a first condition in a first region matching the target location information, and the acquired keyword is used as a first keyword. The first keyword is provided for presentation in the search page.