Abstract: The present disclosure belongs to a promotion system for cellular protein synthesis and use thereof, and specifically relates to the technical fields of cell metabolic engineering and synthetic biology. In the present disclosure, a RNA-binding protein (RBP) AbrP is provided. On one hand, the AbrP binds to translation-promoting factors FusA, LepA, and Era separately; on the other hand, the AbrP binds to a characteristic sequence of an sRNA. A complex resulting from the combination of the AbrP and the promoting factor reaches a translation system of a target mRNA under a guidance of the sRNA, thus releasing the translation-promoting factors FusA, LepA, and Era to stimulate and promote the translation. The present disclosure can solve the current urgent need and practical application for a regulatory element or regulatory system in a positive regulatory mode in the cell metabolic engineering or synthetic biology.

Abstract: The present disclosure provides a smart insole special for golf, belonging to the technical field of insoles. The smart insole includes a toe portion and a heel portion; inside the toe portion and the heel portion is formed a communicated cavity, the toe portion is formed with a groove, inside which groove is formed a plurality of through holes, the through holes are communicated with the cavity, one end inside the cavity close to the heel portion has a top fixedly connected to a partition block, and the cavity has an area located at a bottom of the heel portion filled with a porous sponge. The present disclosure can be combined with different application scenarios, select different usage modes, and improve the comfort specifically.

Abstract: Provided are a method for establishing a lentiviral vector system capable of directly reflecting type I interferon response, and applications thereof. The method for establishing the lentiviral vector system comprises: cutting a Gaussia luciferase at the position of amino acid 109, removing 16 amino acids from N-terminus, and cloning the two polypeptides into a lentiviral vector to form a lentiviral BiLC expression vector; and cloning a shuttle plasmid of pEntry-IRF3 or pEntry-IRF5 or pEntry-IRF7 by homologous recombination into the lentiviral BiLC expression vector, so as to construct a lentiviral vector IRF3-BiLC or IRF5-BiLC or IRF7-BiLC capable of directly reflecting type I interferon response.

Abstract: Provided are a cell line PA/PB1 HEK293T (Teton) for screening an influenza virus polymerase assembly inhibitors and a construction method thereof. The PA/PB1 HEK293T (Teton) cell line is constructed by integrating a vector expressing PA-GlucC fusion protein and a vector expressing GlucN-PB1 fusion protein into a HEK293T (Teton) cell via a triple plasmid system of pMDLg/pRRE, pRSV-Rev and pMD2.G. The HEK293T (Teton) cell line is formed by transforming a TOP10 competent cell with a synthesized Teton-3G gene fragment ligated to a lentiviral vector FG.

Abstract: Provided are a cell line PA/PB1 HEK293T (Teton) for screening an influenza virus polymerase assembly inhibitors and a construction method thereof. The PA/PB1 HEK293T (Teton) cell line is constructed by integrating a vector expressing PA-GlucC fusion protein and a vector expressing GlucN-PB1 fusion protein into a HEK293T (Teton) cell via a triple plasmid system of pMDLg/pRRE, pRSV-Rev and pMD2.G. The HEK293T (Teton) cell line is formed by transforming a TOP10 competent cell with a synthesized Teton-3G gene fragment ligated to a lentiviral vector FG.

Abstract: Provided are a method for establishing a lentiviral vector system capable of directly reflecting type I interferon response, and applications thereof. The method for establishing the lentiviral vector system comprises: cutting a Gaussia luciferase at the position of amino acid 109, removing 16 amino acids from N-terminus, and cloning the two polypeptides into a lentiviral vector to form a lentiviral BiLC expression vector; and cloning a shuttle plasmid of pEntry-IRF3 or pEntry-IRF5 or pEntry-IRF7 by homologous recombination into the lentiviral BiLC expression vector, so as to construct a lentiviral vector IRF3-BiLC or IRF5-BiLC or IRF7-BiLC capable of directly reflecting type I interferon response.