Patents by Inventor Ziyu Dai

Ziyu Dai has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10947548
    Abstract: This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding ?-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropionate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.
    Type: Grant
    Filed: April 24, 2019
    Date of Patent: March 16, 2021
    Assignee: Battelle Memorial Institute
    Inventors: Shuang Deng, Ziyu Dai, Jon K. Magnuson
  • Publication number: 20190323020
    Abstract: This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding ?-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropironate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.
    Type: Application
    Filed: April 24, 2019
    Publication date: October 24, 2019
    Applicant: Battelle Memorial Institute
    Inventors: Shuang Deng, Ziyu Dai, Jon K. Magnuson
  • Patent number: 9914932
    Abstract: This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.
    Type: Grant
    Filed: November 13, 2014
    Date of Patent: March 13, 2018
    Assignee: Battelle Memorial Institute
    Inventors: Ziyu Dai, Jon K. Magnuson, Shuang Deng, Kenneth S. Bruno, David E. Culley
  • Patent number: 9909152
    Abstract: Fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (LaeA), or both, are described. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also described, as are compositions and kits including the disclosed fungi. Further described are Aspergillus terreus fungi overexpressing the LaeA gene and the use of such fungi for the production of itaconic acid.
    Type: Grant
    Filed: October 30, 2015
    Date of Patent: March 6, 2018
    Assignee: Battelle Memorial Institute
    Inventors: Ziyu Dai, Scott E. Baker
  • Publication number: 20160138031
    Abstract: This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.
    Type: Application
    Filed: November 13, 2014
    Publication date: May 19, 2016
    Applicant: Battelle Memorial Institute
    Inventors: Ziyu Dai, Jon K. Magnuson, Shuang Deng, Kenneth S. Bruno, David E. Culley
  • Publication number: 20160046967
    Abstract: Fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (LaeA), or both, are described. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also described, as are compositions and kits including the disclosed fungi. Further described are Aspergillus terreus fungi overexpressing the LaeA gene and the use of such fungi for the production of itaconic acid.
    Type: Application
    Filed: October 30, 2015
    Publication date: February 18, 2016
    Applicant: BATTELLE MEMORIAL INSTITUTE
    Inventors: Ziyu Dai, Scott E. Baker
  • Patent number: 9206450
    Abstract: Provided herein are fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (Lae), or both. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also provided, as are compositions and kits including the disclosed fungi.
    Type: Grant
    Filed: May 4, 2015
    Date of Patent: December 8, 2015
    Assignee: Battelle Memorial Institute
    Inventors: Ziyu Dai, Scott E. Baker
  • Publication number: 20150232892
    Abstract: Provided herein are fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (Lae), or both. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also provided, as are compositions and kits including the disclosed fungi.
    Type: Application
    Filed: May 4, 2015
    Publication date: August 20, 2015
    Applicant: BATTELLE MEMORIAL INSTITUTE
    Inventors: Ziyu Dai, Scott E. Baker
  • Patent number: 9023637
    Abstract: Provided herein are fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (Lae), or both. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also provided, as are compositions and kits including the disclosed fungi.
    Type: Grant
    Filed: November 30, 2012
    Date of Patent: May 5, 2015
    Assignee: Battelle Memorial Institute
    Inventors: Ziyu Dai, Scott E. Baker
  • Patent number: 8735562
    Abstract: The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.
    Type: Grant
    Filed: October 23, 2008
    Date of Patent: May 27, 2014
    Assignee: Battelle Memorial Institute
    Inventors: Ziyu Dai, Linda L. Lasure, Jon K. Magnuson
  • Publication number: 20090068723
    Abstract: The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.
    Type: Application
    Filed: October 23, 2008
    Publication date: March 12, 2009
    Applicant: BATTELLE MEMORIAL INSTITUTE
    Inventors: Ziyu Dai, Linda L. Lasure, Jon K. Magnuson
  • Publication number: 20090042302
    Abstract: The invention includes isolated polynucleotide molecules that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention includes a method of enhancing a bioprocess utilizing a fungus. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to a promoter. The polynucleotide sequence is expressed to promote a first morphology. The first morphology of the transformed fungus enhances a bioprocess relative to the bioprocess utilizing a second morphology.
    Type: Application
    Filed: September 24, 2008
    Publication date: February 12, 2009
    Applicant: BATTELLE MEMORIAL INSTITUTE
    Inventors: Linda L. Lasure, Ziyu Dai
  • Patent number: 7449569
    Abstract: The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.
    Type: Grant
    Filed: August 17, 2004
    Date of Patent: November 11, 2008
    Assignee: Battelle Memorial Institute
    Inventors: Ziyu Dai, Linda L. Lasure, Jon K. Magnuson
  • Patent number: 7439067
    Abstract: The invention includes isolated polynucleotide molecules that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention includes a method of enhancing a bioprocess utilizing a fungus. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to a promoter. The polynucleotide sequence is expressed to promote a first morphology. The first morphology of the transformed fungus enhances a bioprocess relative to the bioprocess utilizing a second morphology.
    Type: Grant
    Filed: May 19, 2003
    Date of Patent: October 21, 2008
    Assignee: Battelle Memorial Institute
    Inventors: Linda L. Lasure, Ziyu Dai
  • Publication number: 20080085341
    Abstract: Methods and microorganisms for forming fermentation products utilizing a microorganism having at least one heterologous gene sequence that enables carbohydrate conversion and carbon dioxide fixation in the production of the fermentation products are disclosed according to some aspects.
    Type: Application
    Filed: August 29, 2007
    Publication date: April 10, 2008
    Inventors: Ziyu Dai, Linda Lasure, Scott Baker, Jon Magnuson
  • Publication number: 20060040342
    Abstract: The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.
    Type: Application
    Filed: August 17, 2004
    Publication date: February 23, 2006
    Applicant: Battelle Memorial Institute
    Inventors: Ziyu Dai, Linda Lasure, Jon Magnuson
  • Publication number: 20050060775
    Abstract: The invention includes methods for production of a polypeptide having factor VIII activity by introduction of a polynucleotide construct into a plant cell. The construct includes an encoding sequence for a polypeptide of coagulation factor VIII or a functional variant thereof. The plant cell is cultured or regenerated into a plant and the polypeptide or functional variant of factor VIII is expressed therein. The invention also includes vectors, plant cells, plant tissues, plants and seeds containing a polynucleotide sequence encoding a functional variant of human coagulation factor VIII. The invention further includes a recombinant DNA molecule having a promoter which is functional in plants operably linked to a coding sequence which codes for a polynucleotide having coagulation factor VIII activity.
    Type: Application
    Filed: June 8, 2004
    Publication date: March 17, 2005
    Inventors: Brian Hooker, Daniel Anderson, Johnway Gao, Ziyu Dai
  • Publication number: 20050005324
    Abstract: There are disclosed novel pin1 gene promoter isoforms and amt gene promoter isoforms. The promoters and their functional elements may be used independently or in combination with one or more enhancer elements to increase or otherwise manipulate gene expression. The promoters disclosed may be used in Controlled Environment Agriculture for heterologous protein production. Also disclosed are methods for using the promoter and promoter elements of the instant invention, as well as vectors and transgenic plants comprising the same.
    Type: Application
    Filed: July 30, 2004
    Publication date: January 6, 2005
    Inventors: Ziyu Dai, LiFang Shi, Brian Hooker
  • Patent number: 6783978
    Abstract: The promoter of the ribosomal protein gene L25 is operably linked to a structural gene. This chimeric gene is placed in an expression vector. The expression vector containing the chimeric gene is used to transform plants cells and plants. Seeds are obtained from the transformed plants. A product, such as a protein, encoded by the structural gene is isolated from the transformed plant cells and plants.
    Type: Grant
    Filed: November 20, 2000
    Date of Patent: August 31, 2004
    Assignee: Battelle Memorial Institute
    Inventors: Lifang Shi, Johnway Gao, Brian S. Hooker, Ziyu Dai
  • Publication number: 20030215950
    Abstract: The invention includes isolated polynucleotide molecules that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention includes a method of enhancing a bioprocess utilizing a fungus. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to a promoter. The polynucleotide sequence is expressed to promote a first morphology. The first morphology of the transformed fungus enhances a bioprocess relative to the bioprocess utilizing a second morphology.
    Type: Application
    Filed: May 19, 2003
    Publication date: November 20, 2003
    Inventors: Linda L. Lasure, Ziyu Dai