Abstract: Disclosed are the expression of a recombinant chicken IgY scFv antibody and monoclonal antibody raised against human thymidine kinase 1 (hTK1) in prokaryotic cells and a preparation method and use thereof. The scFv antibody includes an amino acid sequence shown in SEQ ID NO: 1. The anti-hTK1 recombinant scFv antibody and monoclonal antibody have good immunoreactivity, high specificity and sensitivity, and are easy to separate and purify.

Abstract: Disclosed are the development of a recombinant chicken IgY monoclonal antibody and a recombinant chicken IgY single-chain variable fragment (scFv) antibody raised against human thymidine kinase 1 in mammalian cells and a preparation method and use thereof. A light chain of the scFv antibody includes an amino acid sequence shown in SEQ ID NO: 1, and a heavy chain of the scFv antibody includes an amino acid sequence shown in SEQ ID NO: 2.

Abstract: Disclosed are the expression of a recombinant chicken IgY scFv antibody and monoclonal antibody raised against human thymidine kinase 1 (hTK1) in prokaryotic cells and a preparation method and use thereof. The scFv antibody includes an amino acid sequence shown in SEQ ID NO: 1. The anti-hTK1 recombinant scFv antibody and monoclonal antibody have good immunoreactivity, high specificity and sensitivity, and are easy to separate and purify.

Abstract: Disclosed are a kit and use thereof. The kit includes a first polyclonal antibody that has been immobilized or suitable for immobilization on a solid carrier, a second polyclonal antibody labeled with a marker. Both the first polyclonal antibody and the second polyclonal antibody are both chicken anti human-thymidine kinase IgY-polyclonal antibodies. Both the first polyclonal antibody and the second polyclonal antibody are suitable for specifically binding to thymidine kinase 1. The kit is suitable for the risk assessment of micro malignant tumors/precancerous diseases and tumors that are not detectable by human population screening images.

Abstract: A wearable equipment is adapted for substation maintenance mobile inspection and includes a binding strap and a controlling wireless communication module. A thermal imager is disposed on one side of the binding strap. A prism display component and a voice interaction module are disposed on the other side of the binding strap. The thermal imager, the prism display component, and a microphone and a speaker of the voice interaction module are connected to the controlling wireless communication module through cables, to transmit information in a power system intranet. An application method includes mounting the wearable equipment to an electric power helmet, and automatically sending an infrared image, a visible light image, power monitoring information, voice, and a video in a power operation area to a background management controlling system for the substation maintenance mobile inspection.

Abstract: A wearable equipment is adapted for substation maintenance mobile inspection and includes a binding strap and a controlling wireless communication module. A thermal imager is disposed on one side of the binding strap. A prism display component and a voice interaction module are disposed on the other side of the binding strap. The thermal imager, the prism display component, and a microphone and a speaker of the voice interaction module are connected to the controlling wireless communication module through cables, to transmit information in a power system intranet. An application method includes mounting the wearable equipment to an electric power helmet, and automatically sending an infrared image, a visible light image, power monitoring information, voice, and a video in a power operation area to a background management controlling system for the substation maintenance mobile inspection.