Abstract: A method for preparing a fish skin mucous gland bioreactor and its application, including: identifying genes specifically expressed in fish skin mucinous gland cells, promoters and secreted protein signal peptides, constructing transgenic expression vectors that can specifically express endogenous or heterologous biologically active substances in fish skin and mucous gland cells, developing stable genetic and transgenic fish that secrete bioactive substances into fish mucus, and using bioactive substances secreted by mucus glands for animal and plant growth, stress resistance and disease resistance, human health care and disease prevention, and commercial enzymes. The fish skin mucous gland bioreactor developed by the invention has the characteristics of easy breeding and expansion, more skin mucus secretion, convenient mucus collection, and easy purification of bioactive substances, and can realize the large-scale production of fish skin mucous gland bioreactor and efficient application.

Abstract: Method for producing YY super-male and XY physiological female common carps, with which YY-chromosome super-male common carp can be cultivated and androgenetic YY super-male common carp is produced, where microsatellite markers are used for paternity testing and test crossing confirmation. The YY common carp is crossed with normal female common carp to produce the progenies of only male common carp, and without sex identification, the juvenile male common carp with known sex are subjected to artificial sex reversal to produce XY physiological female common carp.

Abstract: Method for producing YY super-male and XY physiological female common carps, with which YY-chromosome super-male common carp can be cultivated and androgenetic YY super-male common carp is produced, where microsatellite markers are used for paternity testing and test crossing confirmation. The YY common carp is crossed with normal female common carp to produce the progenies of only male common carp, and without sex identification, the juvenile male common carp with known sex are subjected to artificial sex reversal to produce XY physiological female common carp.

Abstract: A method, a system, and a network device for obtaining cell reselection priority are disclosed. The method includes: The network obtains information for calculating priority; and the network determines a terminal-specific private priority list according to the information, and delivers the determined private priority list specific to the terminal through special signaling; or the terminal obtains all or part of the information for calculating priority from the network, and determines its private priority list according to the obtained information; or both the network and the terminal determine the terminal-specific private priority list according to the information.

Abstract: The invention discloses a method for controlling fish reproduction, the method comprises the following steps of: A, constructing a recombinant gene CMV-eGFP-SV40-CMV-Ga14-SV40 and establishing a GAL4 transgenic zebrafish line; B, constructing a recombinant gene CMV-RFP-SV40-UAS-antisense dnd and establishing a uas-antisensednd transgenic zebrafish line; and C, hybridizing by taking homozygous transgenic fish of the two lines as parents, and the hybrid generation fish was sterility. The method of the present invention takes a strategy that two fertilizable lines hybridized but hybrid generation is sterility, establishing a novel method with a universal meaning for controlling fish fertility; The method solves the contradiction of the reproductively sterility of fish and the hereditability of characters in a reproduction operation research and can be effectively applied to cultivation of new fish specie and population control.

Abstract: The invention discloses a method for controlling fish reproduction, the method comprises the following steps of: A, constructing a recombinant gene CMV-eGFP-SV40-CMV-Ga14-SV40 and establishing a GAL4 transgenic zebrafish line; B, constructing a recombinant gene CMV-RFP-SV40-UAS-antisense dnd and establishing a uas-antisensednd transgenic zebrafish line; and C, hybridizing by taking homozygous transgenic fish of the two lines as parents, and the hybrid generation fish was sterility. The method of the present invention takes a strategy that two fertilizable lines hybridized but hybrid generation is sterility, establishing a novel method with a universal meaning for controlling fish fertility; The method solves the contradiction of the reproductively sterility of fish and the hereditability of characters in a reproduction operation research and can be effectively applied to cultivation of new fish specie and population control.

Abstract: The invention discloses a recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen (DO) stress and the use thereof. Carp ?-actin gene promoter is used as a promoter and Vitreoscilla hemoglobin gene is used as a target gene, so as to construct the recombinant Vitreoscilla hemoglobin gene driven by carp ?-actin promoter. The modeling organism zebrafish is used as the research object, and the recombinant gene is microinjected into zygotes of zebrafish. After PCR screening and 156 h low DO stress test, transgenic fish are obtained with a survival rate of 92%, which is significantly different from the survival rate of 65% of the control fish group. The vhb transgenic zebrafish obtain hypoxia tolerance. When the recombinant gene is applied to the economically farmed species, i.e., blunt snout bream (Megalobrama amblycephala) and common carp (Cyprinus carpio L.), it enhances their hypoxia tolerance as well.

Abstract: The present invention relates to an earthquake and tsunami warning system (ETWS) technology, and in particular to a system, apparatus and methods for broadcasting and transmitting an ETWS message. The system for broadcasting and transmitting an ETWS message includes: an ETWS message publishing entity, adapted to publish an ETWS message; a cell broadcast center (CBC), adapted to: encapsulate the ETWS message published by the ETWS message publication entity into a cell broadcast service (CBS) message, and send the CBS message; a mobility management entity (MME), adapted to forward the CBS message sent by the CBC to a radio access network (RAN); and the RAN, adapted to: receive the CBS message forwarded by the MME, and send the received CBS message to a user equipment (UE). The preceding system provides a new technical solution for transmitting an ETWS message, which can guarantee the security of the ETWS message transmission by using the security mechanism of the CBS.

Abstract: The invention discloses a recombinant gene which enhances the ability of fish to tolerate low dissolved oxygen (DO) stress and the use thereof. Carp ?-actin gene promoter is used as a promoter and Vitreoscilla hemoglobin gene is used as a target gene, so as to construct the recombinant Vitreoscilla hemoglobin gene driven by carp ?-actin promoter. The modeling organism zebrafish is used as the research object, and the recombinant gene is microinjected into zygotes of zebrafish. After PCR screening and 156 h low DO stress test, transgenic fish are obtained with a survival rate of 92%, which is significantly different from the survival rate of 65% of the control fish group. The vhb transgenic zebrafish obtain hypoxia tolerance. When the recombinant gene is applied to the economically farmed species, i.e., blunt snout bream (Megalobrama amblycephala) and common carp (Cyprinus carpio L.), it enhances their hypoxia tolerance as well.

Abstract: The present invention relates to an earthquake and tsunami warning system (ETWS) technology, and in particular to a system, apparatus and methods for broadcasting and transmitting an ETWS message. The system for broadcasting and transmitting an ETWS message includes: an ETWS message publishing entity, adapted to publish an ETWS message; a cell broadcast center (CBC), adapted to: encapsulate the ETWS message published by the ETWS message publication entity into a cell broadcast service (CBS) message, and send the CBS message; a mobility management entity (MME), adapted to forward the CBS message sent by the CBC to a radio access network (RAN); and the RAN, adapted to: receive the CBS message forwarded by the MME, and send the received CBS message to a user equipment (UE). The preceding system provides a new technical solution for transmitting an ETWS message, which can guarantee the security of the ETWS message transmission by using the security mechanism of the CBS.

Abstract: Scheduling System Information (SI) in a radio communication system is disclosed. System Frame Number (SFN) and position of System Information is determined in accordance with the SFN. The SI-x is established, and all SIs on the radio frames are distributed discretely at different times for sending. An SI scheduling system and a UE are also disclosed. The technical solution under the present invention optimizes scheduling of SI, avoids excessive network load caused by sequentially sending of all SIs on different sub-frames, and improves the efficiency of scheduling user data.

Abstract: A method, a system, and a network device for obtaining cell reselection priority are disclosed. The method includes: The network obtains information for calculating priority; and the network determines a terminal-specific private priority list according to the information, and delivers the determined private priority list specific to the terminal through special signaling; or the terminal obtains all or part of the information for calculating priority from the network, and determines its private priority list according to the obtained information; or both the network and the terminal determine the terminal-specific private priority list according to the information.

Abstract: The present application disclosed a method for preparing a sterile transgenic fish, comprising constructing antisense RNA expression vector of salmon-type gonadotropin-releasing hormone, introducing the recombinant DNA fragment into carp oosperm by microinjection, and screening the sterile transgenic fish by Polymerase Chain Reaction and radioimmunoassay, wherein the expression vector comprising a promoter of carp beta actin (?-actin) gene, a complementary DNA fragment of antisense salmon-type gonadotropin-releasing hormone (sGnRH) gene from carp with 323 bp as a target gene comprising sGnRH decapeptide, the coding region of gonadotropin-releasing hormone associated peptide and 3? non-coding sequence, and 3? flanking sequence of grass carp growth hormone gene as a stop sequence.

Abstract: The present application disclosed a method for preparing a sterile transgenic fish, comprising constructing antisense RNA expression vector of salmon-type gonadotropin-releasing hormone, introducing the recombinant DNA fragment into carp oosperm by microinjection, and screening the sterile transgenic fish by Polymerase Chain Reaction and radioimmunoassay, wherein the expression vector comprising a promoter of carp beta actin (?-actin) gene, a complementary DNA fragment of antisense salmon-type gonadotropin-releasing hormone (sGnRH) gene from carp with 323 bp as a target gene comprising sGnRH decapeptide, the coding region of gonadotropin-releasing hormone associated peptide and 3? non-coding sequence, and 3? flanking sequence of grass carp growth hormone gene as a stop sequence.