Abstract: Provided, among other things, is a slowly dissolvable film comprising: herbal bioactive agent(s); and polymer(s), dissolvable in the aggregate, wherein the film becomes adhesive as it is placed against a mucosal surface and begins to absorb moisture therefrom.

Abstract: The present invention provides for proteome fractionation through the separation and accumulation of subcellar organelles from a biological sample such that the subcellular organelles are highly enriched, substantially pure, and whose structural integrity and functions are well-preserved. The methods of the invention provide a manner by which to reduce the complexity of the proteome and facilitate the detection and isolation of difficult-to-study proteins, such as low-abundance proteins. The methods of the present invention for pre-fractioning proteomes of biological samples by parallel separation and isolation of subcellular organelles from the biological samples using continuous-flow ultracentrifugation are also easily and effectively scalable through adjustment to ultracentrifugation parameters, such as, for example, rotor speed, rotor size, rotor geometry.

Abstract: The present invention relates to a novel method for the detection and characterization of an unknown nucleic acid-binding protein from a biological sample. More particularly, the method of the instant invention includes the steps of forming nucleic acid-protein complexes, the subsequent selective degradation of of unbound nucleic acid molecules, the detection of nucleic acid-protein complexes, and finally, the characterization of the nucleic acid-binding protein. Additionally, compositions and methods are provided in the present invention for detecting and characterizing unknown nucleic acid-binding proteins from biological samples using immobilized nucleic acid molecules in reaction vessels having a multitude of wells.

Abstract: The present invention relates to a gravitational flow purification system. More particularly, the invention relates to a process for purifying or isolating one or more substances from samples comprising said substances. Even more particularly, the invention relates to purifying or isolating macromolecules from biological samples using a gravitational flow apparatus.

Abstract: The present invention provides an integrated structural unit that includes a diagnostic form that includes at least one active ingredient that is present in an amount that advantageously does not vary by more than about five percent from a predetermined target amount. In one embodiment, the unit form comprises a substrate, a deposit that is disposed on the substrate, and a spreading layer that overlies the deposit and is used to retain and spread a sample of liquid which is to be assayed. The deposit comprises a powder, including the active ingredient(s). The diagnostic form is created via a dry powder deposition apparatus that electrostatically deposits the powder on the substrate utilizing an electrostatic chuck and charged powder delivery apparatus.

Abstract: The present invention provides an integrated structural unit that includes a diagnostic form that includes at least one active ingredient that is present in an amount that advantageously does not vary by more than about five percent from a predetermined target amount. In one embodiment, the unit form comprises a substrate, a deposit that is disposed on the substrate, and a spreading layer that overlies the deposit and is used to retain and spread a sample of liquid which is to be assayed. The deposit comprises a powder, including the active ingredient(s). The diagnostic form is created via a dry powder deposition apparatus that electrostatically deposits the powder on the substrate utilizing an electrostatic chuck and charged powder delivery apparatus.

Abstract: The nucleic acid amplification reactions of the present invention are conducted isothermally, using non-thermal denaturation procedures, such as occurs in chemical- or electrostatic-based denaturation. Embodiments are set forth for automated forms of the claimed procedures.

Abstract: The present invention relates to a method for capturing a class of nucleic acids comprising (a) providing a population of nucleic acids, wherein the population comprises the class; (b) binding a probe moiety to nucleic acids of the class, thereby forming one or more complexes, wherein the probe moiety is attached to a substrate; and (c) capturing the complex. The method can be implemented in certain embodiments in a structure comprised of one or more reservoirs, preferably wherein the reservoirs include one or more second chambers that are in communication with a first chamber. Preferably, the present invention is used to capture the class of nucleic acids that comprise regulatory elements included in a population of nucleic acids isolated from a cell, tissue, or organism. An alternative preferred embodiment of the present invention involves the capture of the class of nucleic acids that comprises certain repetitive elements included in a population of nucleic acids derived from an organism.

Abstract: In one aspect, the present invention relates to methods of amplifying a target nucleic acid, the methods involving the production of a double-stranded promoter to provide for transcription of the target sequence, and the provision of a poly-A tail in the transcription product. In another aspect, the present invention relates to methods of detecting a target nucleic acid in a sample.