Abstract: This disclosure provides a method for detecting and/or identifying uncultured bacteria. The sample is an aerosol sample selected from a group consisting of cough, sneeze, saliva, mucus, bile, urine, vaginal secretions, middle ear aspirate, pus, pleural effusions, synovial fluid, abscesses, cavity swabs, serum, blood and spinal fluid. The method comprises obtaining absorption spectra (AS) of the sample, extracting and processing the acquired data, thereby detecting and/or identifying the bacteria.

Abstract: The present invention provides a method for detecting and/or identifying specific bacteria within an uncultured sample, comprising steps of: a. obtaining an absorption spectrum (AS) of said uncultured sample; b. acquiring the n dimensional volume boundaries for said specific bacteria; c. data processing said AS; i. noise reducing; ii. extracting m features from said entire AS; iii. dividing said AS into several segments according to said m features; iv. calculating m1 features of each of said segment; and, d. detecting and/or identifying said specific bacteria if said m1 features and/or said m features are within said n dimensional volume; wherein said bacteria is a antibiotics resistance bacteria.