Patents by Inventor Zygmunt Gryczynski
Zygmunt Gryczynski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Multiple-pulse pumping for enhanced fluorescence detection and molecular imaging in cells and tissue
Patent number: 9612245Abstract: The present invention includes a method and system for enhancing the signal-to-noise ratio in emission detection comprising: selecting a probe capable of at least one of fluorescence, phosphorescence, or delayed fluorescence in or about a sample that comprises interfering background signal; and exposing the probe to one or more controllable bursts, each burst comprising two or more pulses, wherein the one or more controllable bursts of high repetition energy pulses enhance the signal from the probe above that of the background signal.Type: GrantFiled: July 3, 2014Date of Patent: April 4, 2017Assignees: University of North Texas Health Science Center at Fort Worth, Texas Christian UniversityInventors: Ryan M. Rich, Ignacy Gryczynski, Julian Borejdo, Zygmunt Gryczynski -
Patent number: 9388450Abstract: The present invention includes compositions and methods for fluorescence-based multiplex probe to simultaneously detect one or more enzymatic activities comprising: a first enzymatic target having a first end and a second end, wherein the first end of the first enzymatic target is attached to a central body and the second end of the first enzymatic target is attached to a first fluorophore; a second enzymatic target having a first end and a second end, wherein the first end of the second enzymatic target is attached to the central body and the second end of the second enzymatic target is attached to a third fluorophore; wherein the central body comprises at least one second fluorophore; wherein the first enzymatic target comprises a specific cleavage site of a first enzyme that cleaves the first enzymatic target; and wherein the second enzymatic target comprises a specific cleavage site of a second enzyme.Type: GrantFiled: March 12, 2014Date of Patent: July 12, 2016Assignees: UNIVERSITY OF NORTH TEXAS HEALTH SCIENCE CENTER, TEXAS CHRISTIAN UNIVERSITYInventors: Zygmunt Gryczynski, Ignacy Gryczynski, Rafal Fudala, Badri P. Maliwal
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Patent number: 9376315Abstract: The present invention provides partially fluorescent nanoparticles and methods of making and using the partially fluorescent nanoparticle having a nanoparticle with a matrix and a fluorescent dye dispersed in or about the matrix, wherein at least a portion of the fluorescent dye has been anisotropically bleached.Type: GrantFiled: February 17, 2011Date of Patent: June 28, 2016Assignee: University of North Texas Health Science Center at Fort WorthInventors: Zygmunt Gryczynski, Ignacy Gryczynski, Rafal Luchowski, Julian Borejdo
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MULTIPLE-PULSE PUMPING FOR ENHANCED FLUORESCENCE DETECTION AND MOLECULAR IMAGING IN CELLS AND TISSUE
Publication number: 20150011406Abstract: The present invention includes a method and system for enhancing the signal-to-noise ratio in emission detection comprising: selecting a probe capable of at least one of fluorescence, phosphorescence, or delayed fluorescence in or about a sample that comprises interfering background signal; and exposing the probe to one or more controllable bursts, each burst comprising two or more pulses, wherein the one or more controllable bursts of high repetition energy pulses enhance the signal from the probe above that of the background signal.Type: ApplicationFiled: July 3, 2014Publication date: January 8, 2015Inventors: Ryan M. Rich, Ignacy Gryczynski, Julian Borejdo, Zygmunt Gryczynski -
Publication number: 20140274797Abstract: The present invention includes compositions and methods for fluorescence-based multiplex probe to simultaneously detect one or more enzymatic activities comprising: a first enzymatic target having a first end and a second end, wherein the first end of the first enzymatic target is attached to a central body and the second end of the first enzymatic target is attached to a first fluorophore; a second enzymatic target having a first end and a second end, wherein the first end of the second enzymatic target is attached to the central body and the second end of the second enzymatic target is attached to a third fluorophore; wherein the central body comprises at least one second fluorophore; wherein the first enzymatic target comprises a specific cleavage site of a first enzyme that cleaves the first enzymatic target; and wherein the second enzymatic target comprises a specific cleavage site of a second enzyme.Type: ApplicationFiled: March 12, 2014Publication date: September 18, 2014Inventors: Zygmunt Gryczynski, Ignacy Gryczynski, Rafal Fudala, Badri P. Maliwal
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Patent number: 8159676Abstract: The present invention includes methods for ratiometric detection of analytes by surface plasmon coupled emission detection that includes disposing a target on the metal layer of a surface plasmon resonance detection system; coupling a first analyte to a first fluorescent dye and a second analyte to a second fluorescent dye; contacting the first and second analytes to the target on the surface plasmon resonance detection system; and measuring the intensity of a first and a second surface plasmon resonance enhanced fluorescence emission ring, wherein the first and second rings, respectively, quantitatively represents the amount of first and second analyte within 50 nanometers of the metal surface.Type: GrantFiled: January 20, 2009Date of Patent: April 17, 2012Assignee: University of North Texas, Health Science Center at Fort WorthInventors: Zygmunt Gryczynski, Ignacy Gryczynski, Evgenia Matveeva, Julian Borejdo
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Publication number: 20110207614Abstract: The present invention provides partially fluorescent nanoparticles and methods of making and using the partially fluorescent nanoparticle having a nanoparticle with a matrix and a fluorescent dye dispersed in or about the matrix, wherein at least a portion of the fluorescent dye has been anisotropically bleached.Type: ApplicationFiled: February 17, 2011Publication date: August 25, 2011Applicant: University of North Texas Health Science Center at Fort WorthInventors: Zygmunt Gryczynski, Ignacy Gryczynski, Rafal Luchowski, Julian Borejdo
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Patent number: 7956989Abstract: The present invention includes a microscope and a method for using the microscope for single molecule with reduced photobleaching of a fluorophore (20) that includes a light translucent material (16); a metal layer (18) disposed on the light translucent material (16); a medium (15) disposed on the metal layer (18), the medium (15) having one or more fluorophores (20) capable of binding a target analyte (e.g.Type: GrantFiled: January 22, 2008Date of Patent: June 7, 2011Assignee: University of North Texas Health Science Center at Fort WorthInventors: Zygmunt Gryczynski, Ignacy Gryczynski, Nils Calander, Julian Borejdo
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Publication number: 20110089317Abstract: The present invention describes the development of thin film calibration strips for microscopy/spectroscopy systems and a simple method/routine to conduct instrument calibration using partially (uniaxially) oriented strip to calibrate microscopy system without the prior knowledge of exact polarization of the strip. The invention describes results from studies including a styryl derivative (LDS 798) embedded in poly(vinyl alcohol) (PVA) film. These films were progressively stretched up to 8 folds. Vertical and horizontal components of absorptions and fluorescence were measured and dichroic ratios were determined for different film stretching ratios. The stretched films have high polarization values for isotropic excitation. The isotropic and stretched PVA films doped with LDS 798 can be used as etalons in near infra red (NIR) spectroscopic measurements.Type: ApplicationFiled: October 21, 2010Publication date: April 21, 2011Applicant: University of North Texas Health Science Center at Forth WorthInventors: Ignacy Gryczynski, Zygmunt Gryczynski, Rafal Luchowski, Julian Borejdo
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Publication number: 20090218516Abstract: The present invention includes methods for ratiometric detection of analytes by surface plasmon coupled emission detection that includes disposing a target on the metal layer of a surface plasmon resonance detection system; coupling a first analyte to a first fluorescent dye and a second analyte to a second fluorescent dye; contacting the first and second analytes to the target on the surface plasmon resonance detection system; and measuring the intensity of a first and a second surface plasmon resonance enhanced fluorescence emission ring, wherein the first and second rings, respectively, quantitatively represents the amount of first and second analyte within 50 nanometers of the metal surface.Type: ApplicationFiled: January 20, 2009Publication date: September 3, 2009Applicant: University of North Texas Health Science Center at Fort WorthInventors: Zygmunt Gryczynski, Ignacy Gryczynski, Evgenia Matveeva, Julian Borejdo
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Publication number: 20080231834Abstract: The present invention includes a microscope and a method for using the microscope for single molecule with reduced photobleaching of a fluorophore (20) that includes a light translucent material (16); a metal layer (18) disposed on the light translucent material (16); a medium (15) disposed on the metal layer (18), the medium (15) having one or more fluorophores (20) capable of binding a target analyte (e.g.Type: ApplicationFiled: January 22, 2008Publication date: September 25, 2008Applicant: University of North Texas Health Science Center at Fort WorthInventors: Zygmunt Gryczynski, Ignacy Gryczynski, Nils Calander, Julian Borejdo
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Patent number: 7400397Abstract: The present invention is directed to optical structures and methods detecting the fluorescence of a molecule using metal-enhanced fluorescence. In particular, the invention describes the use of surface plasmon excitation for excitation of fluorophores near the metal surface and the efficient collection of the emission by coupling into the plasmon resonance and directing towards the detector. More particularly, the present invention makes use of the unique directionality of the plasmon induced fluorescence signal. The present invention is directed to optical structures using metal enhanced fluorescence including: an optical fiber having a conductive external coating; a light emitting diode (LED) having a conical shaped depression with curved sides on a front end surface, the curved sides having a conducting coating on the outer surface with respect to the LED.Type: GrantFiled: July 18, 2006Date of Patent: July 15, 2008Assignee: University of Maryland, BaltimoreInventors: Joseph Lakowicz, Zygmunt Gryczynski, Chris Geddes
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Patent number: 7095502Abstract: The present invention is directed to optical structures and methods detecting the fluorescence of a molecule using metal-enhanced fluorescence. In particular, the invention describes the use of surface plasmon excitation for excitation of fluorophores near the metal surface and the efficient collection of the emission by coupling into the plasmon resonance and directing towards the detector. More particularly, the present invention makes use of the unique directionality of the plasmon induced fluorescence signal. The present invention is directed to optical structures using metal enhanced fluorescence including: an optical fiber having a conductive external coating; a light emitting diode (LED) having a conical shaped depression with curved sides on a front end surface, the curved sides having a conducting coating on the outer surface with respect to the LED.Type: GrantFiled: August 5, 2003Date of Patent: August 22, 2006Inventors: Joseph Lakowicz, Zygmunt Gryczynski, Chris Geddes
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Publication number: 20050053974Abstract: Methods and apparatus for fluorescence detection which can increase sensitivity by as much as 20 to 1000-fold are described. This method can preferably also decrease the contribution of sample autofluorescence to the detected signal. The method uses coupling of excited fluorophores with the surface plasmon resonance present in thin conductive films, for example silver, gold, aluminum, copper, or the like. The phenomenon of surface plasmon-coupled emission (SPCE) occurs for fluorophores in a volume adjacent to the conductive layer. This interaction is independent of the mode of excitation, that is, does not require evanescent wave or surface-plasmon excitation. However, such modes of excitation can be advantageous. SPCE can occur over a narrow angular distribution, converting normally isotropic emission into easily collected directional emission.Type: ApplicationFiled: May 20, 2004Publication date: March 10, 2005Applicant: University of MarylandInventors: Joseph Lakowicz, Joanna Malicka, Ignacy Gryczynski, Zygmunt Gryczynski
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Publication number: 20050031536Abstract: Methods and compositions for the detection and monitoring of drug therapy are provided. In particular, efficient and sensitive methods for the detection of drug ingestion are provided for determining whether individuals are complying with prescribed therapeutic regimens, and for providing a mechanism for identifying drug-resistant strains of infectious agents. The claimed methods and compositions involve the application of transdermal devices containing detection mechanisms for receiving and recording signals generated by the ingestion of a labeled drug. Such devices are attached to the skin for the duration of drug therapy and compliance is determined either by direct reading, or by remote monitoring whereby signals are transmitted from the device and received at an external site such as a healthcare facility.Type: ApplicationFiled: July 7, 2004Publication date: February 10, 2005Inventors: Zygmunt Gryczynski, Candace McCombs, Joseph Lakowicz, Omoefe Abugo
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Publication number: 20040160606Abstract: The present invention is directed to optical structures and methods detecting the fluorescence of a molecule using metal-enhanced fluorescence. In particular, the invention describes the use of surface plasmon excitation for excitation of fluorophores near the metal surface and the efficient collection of the emission by coupling into the plasmon resonance and directing towards the detector. More particularly, the present invention makes use of the unique directionality of the plasmon induced fluorescence signal. The present invention is directed to optical structures using metal enhanced fluorescence including: an optical fiber having a conductive external coating; a light emitting diode (LED) having a conical shaped depression with curved sides on a front end surface, the curved sides having a conducting coating on the outer surface with respect to the LED.Type: ApplicationFiled: August 5, 2003Publication date: August 19, 2004Applicant: UNIVERSITY OF MARYLAND, BALTIMOREInventors: Joseph Lakowicz, Zygmunt Gryczynski, Chris Geddes
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Patent number: 6663846Abstract: Methods and compositions for the detection and monitoring of drug therapy are provided. In particular, efficient and sensitive methods for the detection of drug ingestion are provided for determining whether individuals are complying with prescribed therapeutic regimens, and for providing a mechanism for identifying drug-resistant strains of infectious agents. The claimed methods and compositions involve the application of transdermal devices containing detection mechanisms for receiving and recording signals generated by the ingestion of a labeled drug. Such devices are attached to the skin for the duration of drug therapy and compliance is determined either by direct reading, or by remote monitoring whereby signals are transmitted from the device and received at an external site such as a healthcare facility.Type: GrantFiled: December 21, 1999Date of Patent: December 16, 2003Inventors: Candace McCombs, Joseph R. Lakowicz, Omoefe Abugo, Zygmunt Gryczynski
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Patent number: 6660379Abstract: The steady state and time resolved luminescence spectral properties of two types of novel CdS nanoparticles and nanoparticles are described. CdS nanoparticles formed in the presence of an amine-terminated dendrimer show blue emission. The emission wavelength of these nanoparticles depended on the excitation wavelength. The CdS/dendrimer nanoparticles display polarized emission with the anisotropy rising progressively from 340 to 420 nm excitation, reaching a maximal anisotropy value in excess of 0.3. A new constant positive polarized emission from luminescent nanoparticles is also described. Polyphosphate-stabilized CdS nanoparticles are described that display a longer wavelength red emission maximum than bulk CdS and display a zero anisotropy for all excitation wavelengths. Both nanoparticles display strongly heterogeneous intensity decays with mean decay times of 93 ns and 10 &mgr;s for the blue and red emitting particles, respectively.Type: GrantFiled: July 25, 2001Date of Patent: December 9, 2003Assignee: University of Maryland, BaltimoreInventors: Joseph R. Lakowicz, Ignacy Gryczynski, Zygmunt Gryczynski
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Patent number: 6395556Abstract: The present invention relates to the determination of the presence or concentration of an analyte in a sample by visual or electronic element, using polarization based sensing techniques (14) employing fluorescent sensing (11) and reference molecules (10).Type: GrantFiled: August 7, 2001Date of Patent: May 28, 2002Inventors: Joseph R. Lakowicz, Ignacy Gryczynski, Zygmunt Gryczynski