Live attenuated infectious laryngotracheitis virus (ILTV) vaccines and preparation thereof

- UNIVERSITY OF DELAWARE

This disclosure describes a modified live infectious laryngotracheitis virus (ILTV) developed from a strain of ILTV grown at a reduced incubation temperature, vaccines that include the modified live ILTV, methods for producing the live modified ILTV, and methods that include administering the modified ILTV to a subject.

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Description
CROSS-REFERENCE TO RELATED APPLICATION

This application is the § 371 U.S. National Stage of International Application No. PCT/US2014/062823, filed 29 Oct. 2014, which claims priority to U.S. Provisional Patent Application No. 61/896,729, filed Oct. 29, 2013, each of which is incorporated herein by reference.

GOVERNMENT FUNDING

This invention was made with government support under 2013-31100-0610 awarded by the U.S. Department of Agriculture. The government has certain rights in the invention.

SEQUENCE LISTING

This application contains a Sequence Listing electronically submitted to the United States Patent and Trademark Office via EFS-Web as an ASCII text file entitled “458-00060201_SequenceListing_ST25.txt” having a size of 272 KB and created on Oct. 29, 2014. Due to the electronic filing of the Sequence Listing, the electronically submitted Sequence Listing serves as both the paper copy required by 37 CFR § 1.821(c) and the CRF required by § 1.821(e). The information contained in the Sequence Listing is incorporated by reference herein.

SUMMARY

This disclosure describes, in one aspect a modified live infectious laryngotracheitis virus (ILTV) developed from a strain of ILTV grown at a reduced incubation temperature. The ILTV strain may be of tissue culture origin (TCO) or chick embryo origin (CEO). In particular embodiments, the strain of ILTV can be tissue culture origin (TCO). The modified live strain is serially passaged at a temperature in the range of 28° C. to 31° C., which is lower than the typical growth temperature (37° C.) for producing ILTV, for about 30-50 passages through a suitable culture medium. In some embodiments, the modified strain is generated by serially passaging at a temperature of 30° C. In some embodiments, the strain is produced through 34-44 serial passages. In some embodiments, the culture medium may be chicken embryos or cell culture. In some of these embodiments, the culture medium is cell culture.

In some embodiments, the attenuated ILTV can possess at least one genetic modification compared to the starting ILTV and/or a wild-type ILTV. In some embodiments, the genetic modification can include deletion of a thymine in the coding region for UL50. Such a deletion can result in a frame shift at Asn10 of UL50.

In another aspect, this disclosure describes a vaccine for poultry. The vaccine includes an effective amount of a modified live ILTV. The vaccine may further include a pharmaceutically acceptable diluent, carrier, and/or adjuvant. The vaccine may be useful for immunizing poultry. In some embodiments, the poultry can include a chicken. The vaccine may be formulated for administering to poultry by any suitable route. In some embodiments, the vaccine may be administered via an oral, an ocular (eye drop), an intranasal, an aerosol (spray), or an ingestion (drinking water) route. In particular embodiments, the vaccine may be administered via an aerosol (spray) or drinking water administration, which can be more cost effective for commercial poultry production than other routes of administration. In some embodiments, oral administration of the modified live ILTV to a subject produces increased weight gain and/or decreased sign of infectious laryngotracheitis disease following challenge with a virulent ILTV, compared with an unvaccinated control subject.

The above summary of the present invention is not intended to describe each disclosed embodiment or every implementation of the present invention. The description that follows more particularly exemplifies illustrative embodiments. In several places throughout the application, guidance is provided through lists of examples, which examples can be used in various combinations. In each instance, the recited list serves only as a representative group and should not be interpreted as an exclusive list.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1. ILTV lesions, also referred to as plaques or pocks, on the chorioallantoic membrane (CAM) of the embryonating chicken egg at five days following inoculation. (A) Appearance of unique ILTV lesions on the CAM following inoculation with clone 5-7. Note the small well defined plaques. (B) Appearance of a typical ILTV plaque on the CAM. Note diffuse spreading lesion following inoculation with experimental vaccine P32 37C.

 DETAILED DESCRIPTION OF ILLUSTRATIVE EMBODIMENTS

The disclosure relates to live attenuated infectious laryngotracheitis viruses (ILTVs) and the use of the live attenuated ILTVs as vaccines for poultry. Poultry such as, for example, broiler chickens, vaccinated with the vaccine may have a lower incidence of clinical disease signs and/or increased growth (as measured, for example, by greater weight gain), than poultry vaccinated with chick embryo origin (CEO) vaccines. Also, poultry vaccinated using the methods and vaccines described herein can have improved weight gain compared an unvaccinated poultry after being challenged with a virulent challenge strain of ILTV.

Modified live vaccines of two types have been available for many years to control infectious laryngotracheitis (ILT): vaccines of chick embryo origin (CEO) and vaccines of tissue culture origin (TCO). The CEO products often exhibit negative side effects such as, for example, causing disease, reducing growth rates (exhibited as reduce weight gain), and reducing feed efficiency. Because of the side effect produced by the CEO vaccines, poultry producers may prefer not to use them unless it becomes absolutely necessary to do so. Thus, many flocks go unvaccinated and are susceptible to the disease.

There are limited options for commercially available TCO vaccines. A TCO product is typically milder than a CEO vaccine, but is typically administered via the ocular route. This severely limits its practical commercial use by poultry producers because the vaccine must be instilled into the conjunctiva of each individual bird of a flock. This “eye drop” application can be laborious and expensive. Thus, poultry producers disfavor the process and, consequently, TCO vaccines.

More recently, a third type of vaccine, a recombinant ILTV vaccine, has been commercialized. A recombinant ILTV product can express one or more immunogenic proteins, but not the complete virus, and thus fail to produce side effects associated with CEO vaccines. A disadvantage of recombinant vaccines, however, is that they can be expensive to purchase compared to live vaccines, and are only partially effective. If a safer and effective live vaccine could be developed, the cost of vaccine production would dramatically reduce costs to control ILT.

The new vaccine described herein can replace the use of existing modified live and recombinant vaccines. For example, in commercial use with broiler chicken production, a vaccine prepared with the live attenuated ILTV described herein can offer a cheaper alternative to recombinant vaccines and be safer than current modified live vaccines. As another example, in commercial production of breeder chickens and/or layer chickens, the live attenuated ILTV described herein can be a component of a primary vaccine could be used for primary vaccination, which can be followed with one or more booster vaccinations using, for example, currently available CEO vaccines.

In one embodiment, a commercially available vaccine referred to as a tissue culture origin (TCO) vaccine (LT-IVAX, Merck Animal Health, Summit, N.J.), was serially passaged up to approximately 40 times in successive cultures of chicken kidney cells (CKC) in cell culture maintained at 30° C., an incubation temperature lower than the typical culture temperature used to grow ILTV (37° C.). The virus was then cloned in CKC culture at 30° C. by limiting dilution to obtain a more homogeneous virus population than is typically produced without limiting dilution. The more homogeneous virus population can produce more consistent vaccine characteristics when administered to poultry.

Most poultry vaccines are grown at 37° C. As just discussed above, the ILTV described herein (P32 30C) was produced serially passaging the virus in culture at a temperature of 30° C. A corresponding ILTV (P32 37C) was prepared similarly to the P32 30C ILTV except that it was generated by serially passaging the virus in a culture maintained at 37° C. The resulting experimental vaccines were tested in chickens and found to be safe and efficacious by the ocular/eye drop and oral routes of administration.

TABLE 1 Post-vaccination assessment of ILTV commercial and experimental vaccines in two-week-old broiler chickens vaccinated by the ocular or oral routes. Weight Gain From 1-6 Days Post Vaccination Clinical Disease As a % of Non-Vacc. Signs Associated Group Treatment Weight Gain With Vaccination Combined Bodyweight & Clinical Disease Signs Data Following Ocular or Oral Vaccination 1 TCOA Commercial 94 6/15 2 CEOB Commercial 92 12/15  3 P32 37 C.C 87 4/15 4 P32 30 C.D 92 1/15 5 No Vaccine 100 0/15 Bodyweight & Clinical Disease Signs Data Post Ocular Vaccination 1 TCO Commercial 89 2/5  2 CEO Commercial 93 5/5  3 P32 37 C. 105 1/5  4 P32 30 C. 103 0/5  5 No Vaccine 100 0/5  Bodyweight & Clinical Disease Signs Data Post Oral Vaccination 1 TCO Commercial 96 4/10 2 CEO Commercial 92 7/10 3 P32 37 C. 78 3/10 4 P32 30 C. 87 1/10 5 No Vaccine 100 0/10 ATCO = Tissue culture origin (LT-IVAX, Merck Animal Health, Summit, NJ). BCEO = Chicken embryo origin (TRACHIVAX, Merck Animal Health, Summit, NJ). CP32 37 C. = 32 tissue culture passages performed at 37° C. DP32 30 C. = 32 tissue culture passages performed at 30° C.

Vaccination with commercial (TCO Commercial and CEO Commercial) and experimental (P32 37C and P32 30C) vaccines exhibited somewhat reduced growth of broiler chickens as measured by bodyweight gain (Table 1). The negative effect of vaccination on bodyweight gain is a general drawback to the use of modified live vaccines for controlling ILTV infections. The negative effect on bodyweight gain is typically offset by improved health of vaccinated birds compared to non-vaccinated birds beyond the period reflected in Table 1.

Another disadvantage to using modified live vaccines is their tendency to produce clinical disease signs following vaccination. The P32 30C vaccine produced the fewest number of chickens with clinical disease signs. Clinical disease signs include, for example, conjunctival swelling and/or reddening, presence of an ocular and/or nasal (nares) exudate, nasal sinus swelling, labored breathing, and/or coughing, and in severe cases, a bloody expectoration.

TABLE 2 Post-vaccination assessment of ILTV commercial and experimental vaccines in two-week-old broiler chickens vaccinated by the ocular or oral routes. Q-PCR and ILT Serum Antibody Data Following Ocular or Oral Vaccination ILTV Serology-Day 14 Q-PCR Values-Day 6 Post Vaccination Post Vaccination ILTV #of Chickens Total Group Antibody with Positive Group Treatment Combined Ocular Oral Titers Titer/Total 1 TCO Commercial 31.6 35.2 29.8 184 3/15 2 CEO Commercial 29.0 27.3 29.9 1003 12/15  3 P32 37C 33.2 34.1 35.9 1301 12/15  4 P32 30C 36.5 39.4 35.1 15 6/15 5 No Vaccine 46.0 46.0 46.0 0 0/15

Quantitative (Q)-PCR values are an indication of the presence of ILTV vaccine in the oral/pharyngeal cavity, a body site where the vaccine may be detected. Values are inversely related to virus levels; Lower Q-PCR values equate to the presence of higher virus genome levels. Regardless of the type of vaccine tested, ILTV was not detected in great quantities on Day 6 post-vaccination.

The findings indicate that chickens given the CEO Commercial vaccine had the highest levels of ILTV-specific genome based on Q-PCR. A difference of 3.0 between Q-PCR values represents a 10-fold difference in the level of detectable viral genome. Chickens vaccinated with the P32 30C vaccine showed the lowest level of ILTV in the oral/pharyngeal cavity, suggesting that the vaccine is more attenuated than the other vaccines tested.

Serum antibody titers for ILTV were measured on day 14 post-vaccination using a commercial ELISA kit (Synbiotics Corp., Kansas City, Mo.). The CEO Commercial and P32 37C vaccines induced the highest antibody titers, detected in 12/15 chickens. This finding, along with the higher incidence of clinical disease signs and weight suppression suggest that the CEO Commercial and P32 37C experimental vaccines were not attenuated—i.e., they were more invasive than the TCO Commercial and P32 30C vaccines. Moreover, ELISA serum antibody titers may not correlate well with protection (Fahey et al. 1990. J. Gen. Virol. 71:2401-2405). Thus, a more attenuated vaccine like the P32 30C vaccine can induce protection (Table 3) even when producing low serum antibody titers.

TABLE 3 Post-challenge assessment of ILTV commercial and experimental vaccines in two-week-old broiler chickens vaccinated by the ocular or oral routes. Weight Gain From 1-6 Birds with Days Post Challenge Clinical Disease Microscopic As a % of Non-Vacc. Signs Associated Pathology Group Treatment Weight Gain With ILTV Infection Eyelid Trachea Bodyweight and Protection (Clinical Signs and Microscopic Pathology) Data After Challenge of Chickens Vaccinated via the Ocular or Oral Routes-Combined Data 1 TCO Commercial 678 0/13  0/13  0/13 2 CEO Commercial 711 0/13  0/13  0/13 3 P32 37 C. 677 0/13  0/13  0/13 4 P32 30 C. 741 0/12  0/13  0/13 5 No Vaccine 100 12/12  12/12  5/12 Bodyweight and Protection (Clinical Signs and Microscopic Pathology) Data After Challenge of Chickens Vaccinated via the Ocular Route 1 TCO Commercial 618 0/5 0/5 0/5 2 CEO Commercial 704 0/5 0/5 0/5 3 P32 37 C. 626 0/5 0/5 0/5 4 P32 30 C. 716 0/5 0/5 0/5 5 No Vaccine 100 12/12  12/12  5/12 Bodyweight and Protection (Clinical Signs and Microscopic Pathology) Data After Challenge of Chickens Vaccinated via the Oral Route 1 TCO Commercial 716 0/8 0/8 0/8 2 CEO Commercial 715 0/8 0/8 0/8 3 P32 37 C. 708 0/8 0/8 0/8 4 P32 30 C. 753 0/7 0/8 0/8 5 No Vaccine 100 12/12  12/12  5/12

All vaccines induced protection as measured by weight gain from days 1-6 after challenge, the lack of clinical disease signs, and microscopic pathology observed after ocular and intratracheal route challenge with the virulent USDA challenge strain of ILTV. The Animal and Plant Health Inspection Service of the USDA provides the challenge strain to researchers for the purpose of evaluating immunity of chickens following vaccination with experimental and/or commercial vaccines. It is thus a “standard” strain used for challenge purposes.

Chickens vaccinated with the P32 30C vaccine showed the greatest weight gain, indicating that the broilers were protected. Clinical disease signs observed only in the broilers in the No Vaccine treatment included conjunctival swelling and reddening, presence of an ocular and/or nasal (nares) exudate, labored breathing and coughing. Microscopic pathology of eyelid and trachea was performed to determine whether clinical signs of ILTV infection associated with the virulent challenge were present in these tissues.

Thus, the data in Tables 1-3 demonstrate that the P32 30C vaccine is more attenuated than the other vaccines, based on its ability to produce milder clinical signs. The P32 30C vaccine, however, produced body weight suppression when administered by the oral route, the most common method of vaccine application in commercial practice. For this reason, we decided to clone the P32 30C vaccine using a limit dilution procedure. The limit dilution procedure (Example 2) produced Clone 5-7. Clone 5-7 was evaluated and the results are provided in Tables 4-6. Three serial ten-fold dilutions (10−1, 10−2, and 10−3) of the virus stock were used to vaccinate broiler chickens at two weeks of age. Vaccines were administered by the ocular or oral route.

TABLE 4 Post-vaccination assessment of varying dilutions (10−1-10−3) of ILTV experimental vaccine Clone 5-7 in two-week-old broiler chickens vaccinated by the ocular or oral route. Weight Gain From 1-6 Days Post Vaccination Clinical Disease as a % of Non-Vacc. Signs Associated Group Treatment Weight Gain With Vaccination Combined Bodyweight Data Following Ocular or Oral Vaccination 1 Clone 5-7 94  2/10 Dilution 10−1 2 Clone 5-7 92  0/12 Dilution 10−2 3 Clone 5-7 101  0/12 Dilution 10−3 4 No Vaccine/ Not Applicable Not Applicable Challenge 5 No Vaccine/ 100 Not Applicable No Challenge Bodyweight Data Post Ocular Vaccination 1 Clone 5-7 103 0/5 Dilution 10−1 2 Clone 5-7 94 0/5 Dilution 10−2 3 Clone 5-7 100 0/5 Dilution 10−3 4 No Vaccine/ Not Applicable Not Applicable Challenge 5 No Vaccine/ 100 Not Applicable No Challenge Bodyweight Data Post Oral Vaccination 1 Clone 5-7 85 2/5 Dilution 10−1 2 Clone 5-7 86 0/5 Dilution 10−2 3 Clone 5-7 101 0/5 Dilution 10−3 4 No Vaccine/ Not Applicable Not Applicable Challenge 5 No Vaccine/ 100 Not Applicable No Challenge

Clone 5-7 dilutions given ocularly had minimal impact on chickens 1-6 days post vaccination. Oral administration reduced weight gain at the 10-1 and 10-2 dilutions, but not at the 10-3 dilution. Clinical disease signs were only observed at the highest oral dose (10−1 dilution.

Chickens receiving the 10−3 dilution of the vaccine were free of clinical signs and gained bodyweight at the same rate as chickens not receiving the vaccine or the virulent challenge (“No Vaccine/No Challenge” treatment). Thus, Clone 5-7 demonstrated the potential to be a safe and economically desirable vaccine candidate.

TABLE 5 Post-vaccination assessment of dilutions 10−1 to 10−3 of ILTV experimental vaccine Clone 5-7 in two-week-old broiler chickens vaccinated by the ocular or oral routes. LT Antibody Serology- Day 14 Post Vaccination # of Birds Q-PCR Values- Day 6 ELISA w/Positive # of Birds # of Birds Post Vaccination Titers: Titer: ELISA w/Positive ELISA w/Positive Combined Combined Combined Titers: Titer: Titers: Titer: Group Treatment Group Ocular Oral Group Group Ocular Ocular Oral Oral 1 Clone 5-7 Dilution 10−1 39.2 41.8 36.7 894 6/9  451 2/4 1211 4/5 2 Clone 5-7 Dilution 10−2 37.4 40.9 32.7 786 9/12 584 4/7 1189 4/5 3 Clone 5-7 Dilution 10−3 37.8 37.0 41.4 368 6/12 334 2/7 295 2/5 4 No Vaccine/Challenge Not Not Not 17 0/14 17  0/14 17  0/14 Detected Detected Detected 5 No Vaccine/No Challenge N/A N/A N/A N/A N/A  N/A N/A N/A N/A N/A = Not Applicable

Again, quantitative (Q)-PCR values are an indication of the presence of ILTV vaccine in the oral/pharyngeal cavity, a site where the vaccine may be detected. Values are inversely related to virus genome levels; Lower Q-PCR values equate to the presence of higher virus genome levels. Regardless of the type of vaccine tested, ILTV was not detected in great quantities on Day 6 post-vaccination. A difference of 3.0 between Q-PCR values represents a 10-fold difference in the level of detectable viral genome. Chickens vaccinated with the dilutions of the Clone 5-7 vaccine showed a low level of ILTV in the oral/pharyngeal cavity suggesting the vaccine is attenuated.

Serum antibody titers for ILTV were measured on day 14 post-vaccination using a commercial ELISA kit (Synbiotics Corp., Kansas City, Mo.). Fifty percent or more of chickens vaccinated with the Clone 5-7 vaccine via the ocular or oral route (see Combined Group) produced an ILTV serum antibody response. In each vaccine dilution treatment group, ILTV antibodies were detected in the majority of birds. The data show a greater antibody response in birds receiving the 10−1 and 10−2 dilution vaccines orally compared to chickens given the vaccines via the ocular route. Chickens not given the vaccine (No Vaccine/Challenge treatment) did not produce ILTV serum antibody responses. Once again, however, ELISA titers may not correlate well with protection (Fahey et al. 1990. J. Gen. Virol. 71:2401-2405).

TABLE 6 Post-challenge assessment of dilutions 10−1 to 10−3 of ILTV Clone 5-7 vaccine in two-week-old broiler chickens vaccinated by the ocular or oral route. Weight Gain From 1-6 Days Post Vaccination Clinical Disease As a % of Non-Vacc. Signs Associated Group Treatment Weight Gain With ILTV Infection Combined Bodyweight & Clinical Disease Signs Data Following Ocular or Oral Vaccination 1 Clone 5-7 99 1/8 Dilution 10−1 2 Clone 5-7 92  0/10 Dilution 10−2 3 Clone 5-7 105 1/8 Dilution 10−3 4 No Vaccine/ 61 5/5 Challenge 5 No Vaccine/ 100  0/10 No Challenge Combined Bodyweight & Clinical Disease Signs Data Following Ocular Vaccination 1 Clone 5-7 123 1/3 Dilution 10−1 2 Clone 5-7 94 0/5 Dilution 10−2 3 Clone 5-7 140 1/4 Dilution 10−3 4 No Vaccine/ 61 5/5 Challenge 5 No Vaccine/ 100  0/10 No Challenge Combined Bodyweight & Clinical Disease Signs Data Following Oral Vaccination 1 Clone 5-7 97 0/5 Dilution 10−1 2 Clone 5-7 106 0/5 Dilution 10−2 3 Clone 5-7 99 0/4 Dilution 10−3 4 No Vaccine/ 61 5/5 Challenge 5 No Vaccine/ 100  0/10 No Challenge

After challenge with virulent USDA challenge strain of ILTV, chickens receiving any ILTV Clone 5-7 vaccine dilution had higher weight gain than non-vaccinated/challenged chickens (No Vaccine/Challenge treatment). Following challenge, chickens vaccinated via the ocular or oral routes had fewer clinical disease signs compared to chickens not receiving a vaccination. All chickens vaccinated via the ocular route were protected against clinical disease signs after challenge.

TABLE 7 Post-vaccination assessment of 10−2 dilution of ILTV experimental vaccine Clone 5-7 in five-week-old specific pathogen-free (SPF) chickens vaccinated by the ocular route. Passage 1 and Passage 2. Clinical Disease Signs Observed Bird During 14 Days Q-PCR Ct Value: Number Vaccinated Before Challenge Oral Swabs 5 DPV Passage 1. Oral Vaccine Shed Following Ocular Vaccination Y41 Yes None 32.7* Y42 Yes None 32.5* Y43 Yes None 33*  Y44 Yes None Undetermined Y45 Yes None 35.3  Y46 Yes None 34.5  Y47 Yes None 30.1* Y48 Yes None 35.0  Y49 Yes None 32.4* Y50 Yes None Undetermined Passage 2. Oral Vaccine Shed Following Ocular Vaccination G61 Yes None Undetermined G62 Yes None Undetermined G63 Yes None Undetermined G64 Yes None Undetermined G65 Yes None Undetermined G66 Yes None Undetermined G67 Yes None Undetermined G68 Yes None Undetermined G69 Yes None Undetermined G70 Yes None Undetermined Inoculum Ct Value of 28 (Clone 5-7 diluted 10−2 or 1:100). *Used in pool for Passage 2 inoculum.

In Passage 1, five days post ocular vaccination with the ILTV Clone 5-7 vaccine, minimal shedding was observed in oral swabbings. In Passage 2, virus was not detected five days post vaccination with material collected from Passage 1 birds.

TABLE 8 Post-challenge assessment of 10−2 dilution of ILTV experimental vaccine Clone 5-7 in five-week- old specific pathogen-free (SPF) chickens vaccinated by the ocular route. Passage 1 and Passage 2. Bird Num- Clinical Disease Signs Observed: Days Post Challenge ber Vacc. 1 2 3 4 5 6 7 8 9 10 Passage 1. Post Challenge Data Y41 Yes None None None None None None None None None None Y42 Yes None None None None None None None None None None Y43 Yes None None None None None None None None None None Y44 Yes None None None None None None None None None None Y45 Yes None None None None None None None None None None Y46 Yes None None None None None None None None None None Y47 Yes None None None None None None None None None None Y48 Yes None None None None None None None None None None Y49 Yes None None None None None None None None None None Y50 Yes None None None None None None None None None None B26 No None None None Wet Eye (+1)A   Conj. (+3)B Conj. (+3)  Wet Eye (+1)   None None None B27 No None None None Wet Eye (+1)   Conj. (+2)  Wet Eye (+1)   None None None None B28 No None None None Wet Eye (+1)   None Wet Eye (+1)   None None None None B29 No None None None Wet Eye (+1)   Conj. (+3)  Conj. (+3)  None None None None B30 No None None None Wet Eye (+1)   None None None None None Normal Passage 2. Post Challenge Data G61 Yes None None None None  Conj. (NS)C Conj. (NS) Conj. (NS) None None None G62 Yes None None None Wet eye (+1),  Conj. (NS) Conj. (NS) None None None None NEA G63 Yes None None None None Conj. (NS) Conj. (NS) None None None None G64 Yes None None None  Conj. (+1)B Conj. (NS) Conj. (NS) Conj. (NS) None None None G65 Yes None None None Wet eye (+1)   Conj. (NS) Conj. (NS) None None None None G66 Yes None None None Conj. (+1) Conj. (NS) Conj. (NS) None None None None G67 Yes None None None None Conj. (NS) Conj. (NS) None None None None G68 Yes None None None Conj. (+1) Conj. (NS) Conj. (NS) None None None None G69 Yes None None None Wet eye Conj. (NS) Conj. (NS) None None None None G70 Yes None None None Conj. (+2) Conj. (NS) Conj. (NS) Conj. (NS) None None None P16 No None None None Conj. (+1) Conj. (NS) Conj. (NS) Conj. (NS) Conj. (NS) Wet eye (NS) None P17 No None None None Wet eye (+1)   Conj. (NS) Conj. (NS) None None None None P18 No None None None None Conj. (NS) Conj. (NS) None None None None P19 No None None None None Conj. (NS) Conj. (NS) None None None None P20 No None None None Conj. (+2) Conj. (NS) Conj. (NS) Conj. (NS) None None None AObserved clinical disease signs (severity of clinical disease sign: +1 = mild; +2 = moderate; +3 = severe). BConj. = Conjunctivitis CNS = Not Scored

In Passage 1, following challenge with the ILTV USDA challenge strain, all chickens vaccinated via the ocular route with ILTV Clone 5-7 were protected against clinical disease signs compared to chickens not receiving a vaccination. In Passage 2, there was no difference between birds vaccinated with the oral swab material collected in Passage 1 and the non-vaccinated birds. All birds in Passage 2 showed clinical disease signs compatible with ILTV induced disease. These results show that the ILTV Clone 5-7 is able to induce protective immunity (Passage 1) and does not spread to naïve susceptible chickens.

Thus, this disclosure describes an attenuated infectious laryngotracheitis virus (ILTV) that may be used as a component in a vaccine to immunize a subject against infection by a virulent infectious laryngotracheitis virus. Generally, the attenuated ILTV can be produced by serial passage at a temperature that is reduced from the temperature at which the ILTV is typically grown. Once the attenuated ILTV is so generated, however, one can generate the attenuated ILTV using conventional molecular biology techniques to produce a virus having the same nucleotide and/or functional features of the attenuated ILTV produced as described herein.

While described herein in the context of an exemplary embodiment in which the attenuated ILTV is produced by serial passaging an ILTV virus, the attenuated ILTV described herein—and variants thereof—can be produced by any suitable method. Also, while expressly exemplified herein in the context of an exemplary embodiment in which the attenuated ILTV possesses each of the genetic modifications listed in Table 11, an attenuated ILTV as described herein may contain fewer than all of the genetic mutations listed in Table 11 and still retain an attenuated character.

In some embodiments, the attenuated ILTV can be produced by serial passage of the virus at a minimum temperature of at least 25° C. such as, for example, at least 27° C., at least 28° C., at least 29° C., at least 30° C., at least 31° C., or at least 32° C. In some embodiments, the attenuated ILTV can be produced by serial passage of the virus at a maximum temperature of no more than 35° C. such as, for example, no more than 34° C., no more than 33° C., no more than 32° C., no more than 31° C., no more than 30° C., or no more than 29° C. In some embodiments, the attenuated ILTV can be produced by serial passage of the virus at a temperature within a range having endpoints defined by any minimum temperature listed above and any maximum temperature that is greater than the minimum temperature. For example, in one embodiment, the attenuated virus can be produced by serial passage of virus at a temperature of from 28° C. to 31° C. In one particular embodiment, the attenuated ILTV can be produced by serial passage of the virus at a temperature of 30° C.

The attenuated ILTV can be generated by any suitable number of serial passages. In some embodiments, the attenuated ILTV can be produced by a method that includes a minimum of at least two serial passages of the virus such as, for example, at least three serial passages, at least five serial passages, at least seven serial passages, at least 15 serial passages, at least 25 serial passages, at least 30 serial passages, at least 34 serial passages, at least 40 serial passages, or at least 45 serial passages. In some embodiments, the attenuated ILTV can be produced by a method that includes a maximum of no more than 50 serial passages such as, for example, no more than 44 serial passages, no more than 30 serial passages, no more than 20 serial passages, no more than 10 serial passages, no more than seven serial passages, no more than five serial passages, no more than four serial passages, or no more than three serial passages. In some embodiments, the attenuated ILTV can be produced by a method that includes a number of serial passages within a range having endpoints defined by any minimum number of passages listed above and any maximum number of serial passages that is greater than the minimum number of serial passages. In one particular embodiment, the attenuated ILTV may be generated using a method that includes seven serial passages of the virus. Thus, in some embodiments, the attenuated virus can be produced by a method that includes, for example, 30-50 serial passages such as, for example, 34-44 serial passages.

The serial passaging of the virus at a reduced temperature produces an attenuated virus that includes one or more genetic modifications compared to the ILTV that was the starting material. In the exemplary embodiment described above, the starting ILTV was a commercially available ILTV of tissue culture origin, LT-IVAX (Merck Animal Health, Summit, N.J.). Table 11 lists genetic modifications of the Clone 5-7 generated as described herein compared to the LT-IVAX. Some of the mutations are located in non-coding regions. Others, however, introduce frame shift mutations or amino acid substitutions

As noted above, while exemplified herein in the context of an exemplary embodiment in which the attenuated ILTV possesses each of the genetic modifications listed in Table 11, the attenuated ILTV contemplated herein may contain fewer than all of the genetic mutations listed in Table 11 and still retain an attenuated character. Exemplary genetic modifications can produce, for example, a frame shift mutation in one or more coding regions of the viral genome. Exemplary coding regions that may be affected by a frame shift mutation include, for example, ORF B, ORF F, UL50, UL7, or UL9.

A frame shift mutation in ORF B may include, for example, a frame shift introduced at Phe54 such as may be produced by a deletion of a nucleotide or an addition of a nucleotide in the codon for Phe54 of ORF B. One exemplary mutation is a deletion of a thymine (e.g., the thymine at nucleotide position 23030 of the ILTV genome) from the codon, but a person of ordinary skill in the art can readily conceive of other mutations that could cause a frame shift at Phe54—or any other site—of ORF B.

A frame shift mutation in ORF F may include, for example, a frame shift introduced at Glu233 such as may be produced by a deletion of a nucleotide or an addition of a nucleotide in the codon for Glu233 of ORF F. One exemplary mutation is an addition (e.g., a guanine added between nucleotide position 5377 and 5378 of the ILTV genome) in the codon, but a person of ordinary skill in the art can readily conceive of other mutations that could cause a frame shift at Glu233—or any other site—of ORF F.

A frame shift mutation in UL50 may include, for example, a frame shift introduced at Asn10 such as may be produced by a deletion of a nucleotide or an addition of a nucleotide in the codon for Asn10 of UL50. One exemplary mutation is a deletion of a thymine (e.g., a deletion of the thymine at nucleotide position 15577 of the ILTV genome) from the codon, but a person of ordinary skill in the art can readily conceive of other mutations that could cause a frame shift at Asn10—or any other site—of UL50.

A frame shift mutation in UL7 may include, for example, a frame shift introduced at Phe104 such as may be produced by a deletion of a nucleotide or an addition of a nucleotide in the codon for Phe104 of UL7. One exemplary mutation is a deletion of a adenine (e.g., the adenine at nucleotide position 98125 of the ILTC genome) from the codon, but a person of ordinary skill in the art can readily conceive of other mutations that could cause a frame shift at Phe104—or any other site—of UL7.

A frame shift mutation in UL9 may include, for example, a frame shift introduced at PGlu771 such as may be produced by a deletion of a nucleotide or an addition of a nucleotide in the codon for Glu771 of UL9. One exemplary mutation is a deletion of a adenine (e.g., the adenine at nucleotide position 94565 of the ILTV genome) from the codon, but a person of ordinary skill in the art can readily conceive of other mutations that could cause a frame shift at Glu771—or any other site—of UL9.

In some embodiments, an attenuated ILTV can include a genetic modification to the UL50 coding region without necessarily possessing any of the other genetic modifications shown in Table 11. Moreover, in some embodiments, the genetic modification to UL50 may differ from the thymine deletion identified in Table 11, while still resulting in attenuation of the resulting genetically-modified ILTV.

The attenuated ILTV, when administered to a subject, can decrease the likelihood, extent, and/or severity of infection of the subject by a virulent ILTV compared to a comparable unvaccinated control subject. In some cases, administering the attenuated ILTV can result in increased weight gain and/or a decrease in the severity of one or more signs of infection by a virulent ILTV. Exemplary signs of infection by a virulent ILTV include, for example, conjunctival swelling and/or reddening, presence of an ocular and/or nasal (nares) exudate, nasal sinus swelling, labored breathing, coughing, and in severe cases, a bloody expectoration.

Thus, in another aspect, this disclosure describes a method that involves administering the attenuated infectious laryngotracheitis virus described herein to a subject having or at risk of having an ILTV infection.

Thus, treating a condition can be prophylactic or, alternatively, can be initiated after the subject exhibits one or more symptoms or clinical signs of the condition. As used herein, the term “sign” or “clinical sign” refers to an objective physical finding relating to a particular condition capable of being found by one other than the subject; the term “symptom” refers to any subjective evidence of disease or of a subject's condition. Treatment that is prophylactic—e.g., initiated before a subject manifests a symptom or clinical sign of the condition such as, for example, while an infection remains subclinical—is referred to herein as treatment of a subject that is “at risk” of having the condition. As used herein, the term “at risk” refers to a subject that may or may not actually possess the described risk. Thus, for example, a subject “at risk” of having an infectious condition is a subject present in an area where other individuals have been identified as having the infectious condition and/or is likely to be exposed to the infectious agent. A subject may be “at risk” even if the subject has not yet manifested any detectable indication of infection by a virulent ILTV and regardless of whether the animal may harbor a subclinical amount of a virulent ILTV.

Accordingly, the attenuated ILTV can be administered to a subject before, during, or after the subject first comes in contact with a virulent ILTV. Treatment initiated after the subject first exhibits comes in contact with a virulent ILTV may result in decreasing the severity of symptoms and/or clinical signs of the condition, completely resolving the condition, and/or decreasing the likelihood of experiencing clinical evidence of the condition compared to a subject to which the attenuated ILTV has not been administered.

The method includes administering an effective amount of the attenuated ILTV to a subject exposed to, or at risk of being exposed to ILTV. In this aspect of the invention, an “effective amount” is an amount effective to reduce, limit progression, ameliorate, or resolve, to any extent, the symptoms or clinical signs related to infectious laryngotracheitis.

The attenuated ILTV described herein may be formulated in a composition along with a “carrier.” As used herein, “carrier” includes any solvent, stabilizer, dispersion medium, vehicle, coating, diluent, antibacterial, and/or antifungal agent, isotonic agent, absorption delaying agent, buffer, carrier solution, suspension, colloid, and the like. The use of such media and/or agents for pharmaceutical active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the attenuated ILTV described herein, its use in a therapeutic compositions is contemplated. Supplementary active ingredients also can be incorporated into the compositions.

By “pharmaceutically acceptable” is meant a material that is not biologically or otherwise undesirable, i.e., the material may be administered to an individual along with the attenuated ILTV without causing any undesirable biological effects or interacting in a deleterious manner with any of the other components of the pharmaceutical composition in which it is contained.

The attenuated ILTV may be administered to any suitable subject such as, for example, chickens, turkeys, ducks, pheasants, and peafowl.

In the preceding description, particular embodiments may be described in isolation for clarity. Unless otherwise expressly specified that the features of a particular embodiment are incompatible with the features of another embodiment, certain embodiments can include a combination of compatible features described herein in connection with one or more embodiments.

For any method disclosed herein that includes discrete steps, the steps may be conducted in any feasible order. And, as appropriate, any combination of two or more steps may be conducted simultaneously.

The present invention is illustrated by the following examples. It is to be understood that the particular examples, materials, amounts, and procedures are to be interpreted broadly in accordance with the scope and spirit of the invention as set forth herein.

EXAMPLES Example 1 Generating P32 37C

The P32 37C virus was serially passaged 32 times in primary chicken embryo kidney cells (CEKC), incubated at 37° C.

Kidney cells were prepared from 17-20 day old embryos.

The amounts indicated here are for preparing kidney cells from 10-15 embryos. Eggs were sprayed with a disinfectant (BIOGUARD, Bioguard Hygiene Solutions Ltd., Northampton, UK) and allowed to dry. Kidneys were dissected from the embryos, washed in Phosphate Buffered Saline (PBS) without calcium or magnesium (1.60 g Na2HPO4, 0.51 g KH2PO4, 7.30 g NaCl in 1 L DDH2O). The kidneys were dissociated and treated with trypsin solution (0.25% trypsin in modified PBS+0.02% EDTA, pH 7.4-7.6) and stirred slowly for 15-20 minutes at 37° C. The supernatant was collected by pouring through a gauze-covered funnel into a graduated centrifuge tube with 5 ml of cold (ice bath-cooled) heat-inactivated calf serum. The remaining pellet was retrypsinized with fresh trypsin solution and the process repeated two more times. The collected supernatant was centrifuges at 1500 RPM for 10 minutes. The pelleted kidney cells were resuspended in 3-6 ml minimal essential medium (MEM), then added to MEM with 10% heat-inactivated fetal calf serum.

Cells were counted in a hemacytometer by resuspending in a known amount of media. 2 ml of cells (2.5×106 cells/ml) were plated on a 35 mm2 plate, which formed a monolayer in 1-2 days. When the monolayer formed, the cells were ready for inoculation.

1 mL of starting virus was used to infect one 25 mL flask with 80% confluent monolayer of CEKC's. The inoculated CEKC were incubated at 37° C. ILTV cytopathic effect (CPE) known as syncytia were observed in the CEKC at two days after inoculation. The cells and culture media were harvested and repassaged serially for 32 passages at an incubation temperature of 37° C. A seed stock of the virus was then prepared for further experimentation.

Generating P32 30C

The P32 30C virus was serially passaged 32 times in primary chicken embryo kidney cells (CEKC). Cells were prepared as described above for the preparation of the P32 37C virus, except that the inoculated CEKC were incubated at 30° C. ILTV cytopathic effect (CPE) known as syncytia were observed in the CEKC at 3-4 days after inoculation. The cells and culture media were harvested and repassaged serially for 32 passages at an incubation temperature of 30° C. A seed stock of the virus was then prepared for further experimentation.

Assessment of Vaccines

One-day-old, female commercial broiler chickens (Moyer's Chicks, Inc., Quakertown, Pa.) were assigned to one of five treatment groups shown in Table 9.

TABLE 9 Group Immunizing Vacc. No. of Birds No. Virus Titer (Oral/ED) 1 TCO VaccineA 103.0 10/5 2 CEO VaccineB 104.0 10/5 3 P32 30 C.C 103.0 10/5 4 P32 37 C.D 103.0 10/5 5 Negative Control Sham 10/5 ATCO = Tissue culture origin (LT-IVAX, Merck Animal Health, Summit, NJ). BCEO = Chicken embryo origin (TRACHIVAX, Merck Animal Health, Summit, NJ). CP32 30 C. = 32 tissue culture passages performed at 30° C. DP32 37 C. = 32 tissue culture passages performed at 37° C.

The night prior to vaccination at 14 days of age, water was withheld from the birds. On the afternoon prior to vaccination, all birds were weighed. Birds in the oral vaccinated groups received 1.5 mL of water containing one drop of vaccine (0.03 ml) by hand using a blunt needle and syringe. Vaccination occurred slowly to ensure proper uptake by the bird. Each bird was held vertically, beak pointed slightly toward the ceiling to mimic drinking from a nipple. Each bird in the corresponding eye drop vaccine group was vaccinated by placing one drop (0.03 ml) of inoculum directly into the conjunctival sac.

Birds were observed daily for signs of vaccine reaction (conjunctivitis, nasal discharge). Six days post vaccination, all birds were weighed. Oropharyngeal swabs were collected from all birds to determine the level of ILTV present in the oral cavity by real-time PCR (Callison et al., 2007. J. Virol. Methods 139:31-38). Two birds from each water vaccinated group and the negative control group were euthanized. Trachea and eyelid were collected for evaluation of microscopic pathology.

Fourteen days post vaccination, all birds were weighed, serum samples were collected from all birds prior to challenge with the USDA challenge strain of ILTV (103.5/0.1 ml). ILTV serum antibody titers were evaluated using a commercial ELISA kit (Synbiotics Corp., Kansas City, Mo.). Five days post challenge birds were weighed and evaluated for signs of disease. Trachea and eyelid were collected after euthanizing via cervical dislocation.

Results are shown in Tables 1-3.

Example 2 Generating Clone 5-7 from P 32 30C Using the Limit Dilution Procedure

Serial 10-fold dilutions (10−1-10−6) of the P32 30C virus were prepared and inoculated onto CEKC. The inoculated cells were incubated at 30° C. The CEKC were observed microscopically daily for syncytia. Cells and media were harvested from the highest dilution showing syncytia. A culture plate at the 10−5 dilution, 7th replicate plate met the criteria and the cells and media were harvested. A seed stock was prepared for further experimentation.

Assessment of Vaccines

Female broiler type chickens (Moyer's Chicks, Inc., Quakertown, Pa.). Chicks were placed in isolation in groups of 13 birds. At 20 days of age, birds were water deprived for at least two hours prior to inoculation. The following day, at 21 days of age, birds were randomly regrouped, assigned to treatment groups (Table 10) and necktagged for identification. All birds were weighed. Birds with bodyweights greater than or less than 2SD of the group mean weight were used in the swab control groups. The negative control birds were placed in isolation prior to handling any viruses. Birds in the negative control/oral group received 1 mL of water. No other sham inoculations were performed.

TABLE 10 Cage No. of Female No. Treatment Treatment Broilers 1 Negative Challenge Controls 5 Control Oral 5 Ocular 5 Swab Controls 2 2 10−1 Oral 5 Ocular 5 3 10−2 Oral 5 Ocular 5 Swab Controls 2 4 10−3 Oral 5 Ocular 5 Swab Controls 2 5 10−4 Oral 5 Ocular 5 Swab Controls 2 6 10−5 Oral 5 Ocular 5 Swab Controls 2 7 10−6 Oral 5 Ocular 5 Swab Controls 2

Inoculation:

Prior to inoculation with the Clone 5-7 dilutions, all birds were weighed and blood samples were collected from 10 birds. Six 8-ml tubes were filled with 4.5 ml of sterile water (pH 7.0—spring water). Tubes were used for to create the inoculum dilutions (10−1 to 10−6). Six 10-fold dilutions of the Clone 5-7 ILTV were created by placing 0.5 ml of virus in 4.5 ml of sterile water (pH 7.0—spring water). 0.5 ml of the 10−1 dilution was then be placed into 4.5 ml of sterile water (pH 7.0—spring water) to create the 10−2 and repeated until the 10−6 dilution was created. 0.5 ml were removed from the 10−6 dilution and discarded. All dilution tubes contained 4.5 ml of the virus/water dilution.

Two separate tubes were marked as either for ocular or oral inoculation. The ocular tube contained at least 1 ml of the given dilution. The oral tube contained 0.7 ml of inoculum and 7 ml of water. These tubes were held on ice until used. Ocular treatment birds received 0.1 ml (100 μl) by eyedrop. The oral treatment birds received 1 ml of the water/virus preparation. Swab controls received 0.1 ml (100 μl) of a given vaccine treatment via eyedrop. These birds were used to assess viral load in the trachea by real-time PCR.

6 DPI Swab and Weigh:

Birds were swabbed at six day post-inoculation (dpi) to determine viral load by real time PCR in the oral cavity of the ocular and oral challenged groups. Tracheal and oral swabs were collected from the swab controls to evaluate viral load at both swabbing locations by real-time PCR (Callison et al., 2007. J. Virol. Methods 139:31-38). Body weights were also collected to determine the effect of infection on weight gain. Swabs were collected and placed in 2 ml of sterile Tryptose Phosphate Broth (Difco, BD Biosciences, San Jose, Calif.).

14 DPI-Challenge:

All birds were weighed and bled prior to challenge. The five “challenge controls” in cage 1 were used to verify infection of the broilers using challenge dose of 103 per bird. After challenge, the challenge controls were relocated among the 10−5 and 10−6 treatment groups (cages 6 and 7). All birds in cages 2-7 received a challenge of 103 EID50 of the USDA ILTV challenge strain per 0.1 ml divided between the intraocular (one drop) and oral (remaining volume up to 0.1 ml) routes of inoculation using a 200 μl pipette.

Birds were evaluated daily for signs of clinical disease for a period of 10 days post challenge (DPC). At 6 DPC, birds were weighed to evaluate protection based upon suppression of daily weight gain compared to non-challenged controls. At 10 DPC, birds were weighed and evaluated for signs of clinical disease. All surviving birds were bled to evaluate ILTV antibody status following challenge. ELISA titers were evaluated to determine if they might be indicative of lack of protection or a booster response. The experiment was terminated after 10 DPC.

Results are shown in Tables 4-6.

Genetic Signature of Clone 5-7

Approximately 500 μL of the Clone 5-7 TCO supernatant was treated with DNase and the viral DNA was extracted using the DNeasy 96 Blood & Tissue Kit (Qiagen, Inc., Valencia, Calif.) according the recommended procedures. A DNA library was generated using the NuGen OVATION ultralow library system (NuGEN Technologies, Inc., San Carlos, Calif.). The libraries were sequenced on an Illumina Genome Analyzer IIx (Illumina, Inc., San Diego, Calif.) in a paired-end, 101 cycle run. Library reads were assembled to a reference ILTV genome using CLCWorkbench (CLC bio, Inc., Germantown, Md.). Variant detection within the Clone 5-7 assembled genome and the impact of the detected variation on amino acid sequence was performed within CLCWorkbench (CLC bio, Inc.,).

TABLE 11 ILTV Clone 5-7 Genetic Signature vs. Commercial TCO Vaccine Depth of Coding Nt TCO ILTV Clone Non-synonymous Amino Acid Coverage at Frequency of Region Position# Vaccine Nt 5-7 Nt or Synonymous Change Position Mutation (%) NC 1160 A — Not Applicable Not Applicable 2020 3.81 NC 104594 A — Not Applicable Not Applicable 4493 93.08 NC 109606{circumflex over ( )}109607 — G Not Applicable Not Applicable 1981 72.19 NC 109614 T — Not Applicable Not Applicable 980 6.22 NC 109616{circumflex over ( )}109617 — G Not Applicable Not Applicable 1689 3.02 NC 109622 G — Not Applicable Not Applicable 1450 6.55 ORF B 23030 T — Non-synonymous Phe54fs* 5030 6.92 ORF F 5377{circumflex over ( )}5378 — G Non-synonymous Glu233fs 5489 3.06 UL36 53320 A G Non-synonymous Val2300Ala 4082 16.39 UL37 62826 T C Synonymous Not Applicable 4129 3.44 UL50 15577 T — Non-synonymous Asn10fs 4582 92.45 UL7 98125 A — Non-synonymous Phe104fs 4737 5.3 UL9 94565 A — Non-synonymous Glu771fs 4339 5.49 *fs = frame shift #SEQ ID NO: 1, Genbank accession no. JN580312

Example 3

Female specific pathogen free (SPF) leghorn chickens were obtained at five weeks of age from Charles-River SPAFAS (Norwich, Conn.). Birds were periodically obtained in lots of 15 or 30.

Passage 1

For each passage, birds were assigned to one of two groups: Direct Inoculated (10 birds) or Challenge Controls (5 birds) (Table 7 and Table 8). Each bird was tagged according to treatment group. Clone 5-7 was diluted 1:100 and administered via eye-drop (0.1 mL/bird) to each of the 10 birds in Cage 1. On day 5 post challenge (PC), an oropharyngeal swabbing, culminating in the swabbing of the upper tracheal opening, were collected from each bird and placed into two swab tubes (5 swabs in each tube), each containing 2.5 mL of Tryptose Phosphate Broth (Difco, BD Biosciences, San Jose, Calif.) with antibiotics (penicillin (100 I.U.) and streptomycin (100 μg/ml), with fungizone (2.5 μg/ml)). Tubes were held at −80° C. until need for subsequent challenge.

Birds were observed and scored daily for disease signs (conjunctivitis, labored breathing/gasping, mortality) through day 10 post-direct inoculation. Birds were scored as 0=normal, 1=diseased (respiratory signs, swollen eye), or 2=dead. Swab tubes were screened for the presence of ILTV genome by real-time PCR (Callison et al., 2007. J. Virol. Methods 139:31-38).

Fourteen days post inoculation, the Direct Inoculates (10 birds) and the Challenge Controls (5 birds) were bled to determine ILTV serum antibody status by enzyme-linked immunosorbent assay (ELISA) (Synbiotics Corp., Kansas City, Mo.). All birds were moved to the Clone 5-7 Challenge Cage and challenged via eye-drop with the USDA ILTV Challenge Virus (103.5/bird). All birds were evaluated through 10 days PC for disease signs using the same scoring scale defined above.

Passage 2 to Passage 5

On the defined day of challenge, the two swab tubes collected five days post challenge with the previous passage were thawed at room temperature and centrifuged to pellet any debris. An aliquot of 0.5 ml from each tube was taken and pooled to create the inoculum for birds. The 10 birds in the direct inoculation group received 0.1 ml of the undiluted swab pool material via eye-drop. On day 5 post challenge, an oropharyngeal swabbing (culminating in the swabbing of the upper tracheal opening) was collected from each bird and placed into two swab tubes (5 swabs in each tube) each containing 2.5 ml of Tryptose Phosphate Broth (Difco, BD Biosciences, San Jose, Calif.) with antibiotics (penicillin (100 I.U.) and streptomycin (100 μg/ml) with fungizone (2.5 μg/ml)). Tubes were held at −80° C. until need for subsequent challenge.

Birds were observed and scored daily for disease signs (conjunctivitis, labored breathing/gasping, mortality) through day 10 post-direct inoculation. Birds were scored as described for Passage 1. Swab tubes were screened for the presence of ILTV genome by real-time PCR as described for Passage 1.

Fourteen days post inoculation, the Direct Inoculates (10 birds) and the Challenge Controls (5 birds) were bled to determine ILTV serum antibody status as described for Passage 1. All birds were challenged via eye-drop and evaluated through 10 days post challenge as described for Passage 1.

The inoculation procedure described above was repeated until ILTV was no longer detected by real-time PCR, the birds were susceptible to challenge, or a total of 5 bird passages had been completed.

Results are shown in Tables 7 and 8.

As used herein, the term “and/or” means one or all of the listed elements or a combination of any two or more of the listed elements; the terms “comprises” and variations thereof do not have a limiting meaning where these terms appear in the description and claims; unless otherwise specified, “a,” “an,” “the,” and “at least one” are used interchangeably and mean one or more than one; and the recitations of numerical ranges by endpoints include all numbers subsumed within that range (e.g., 1 to 5 includes 1, 1.5, 2, 2.75, 3, 3.80, 4, 5, etc.).

The complete disclosure of all patents, patent applications, and publications, and electronically available material (including, for instance, nucleotide sequence submissions in, e.g., GenBank and RefSeq, and amino acid sequence submissions in, e.g., SwissProt, PIR, PRF, PDB, and translations from annotated coding regions in GenBank and RefSeq) cited herein are incorporated by reference in their entirety. In the event that any inconsistency exists between the disclosure of the present application and the disclosure(s) of any document incorporated herein by reference, the disclosure of the present application shall govern. The foregoing detailed description and examples have been given for clarity of understanding only. No unnecessary limitations are to be understood therefrom. The invention is not limited to the exact details shown and described, for variations obvious to one skilled in the art will be included within the invention defined by the claims.

Unless otherwise indicated, all numbers expressing quantities of components, molecular weights, and so forth used in the specification and claims are to be understood as being modified in all instances by the term “about.” Accordingly, unless otherwise indicated to the contrary, the numerical parameters set forth in the specification and claims are approximations that may vary depending upon the desired properties sought to be obtained by the present invention. At the very least, and not as an attempt to limit the doctrine of equivalents to the scope of the claims, each numerical parameter should at least be construed in light of the number of reported significant digits and by applying ordinary rounding techniques.

Notwithstanding that the numerical ranges and parameters setting forth the broad scope of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as possible. All numerical values, however, inherently contain a range necessarily resulting from the standard deviation found in their respective testing measurements.

All headings are for the convenience of the reader and should not be used to limit the meaning of the text that follows the heading, unless so specified.

<160> NUMBER OF SEQ ID NOS: 1 <210> SEQ ID NO 1 <211> LENGTH: 155465 <212> TYPE: DNA <213> ORGANISM: Gallid herpesvirus 1 <220> FEATURE:  <221> NAME/KEY: exon <222> LOCATION: (4680)..(6920) <220> FEATURE:  <221> NAME/KEY: exon <222> LOCATION: (14355)..(15605) <220> FEATURE:  <221> NAME/KEY: exon <222> LOCATION: (22871)..(23893) <220> FEATURE:  <221> NAME/KEY: exon <222> LOCATION: (51864)..(60218) <220> FEATURE:  <221> NAME/KEY: exon <222> LOCATION: (60357)..(63422) <220> FEATURE:  <221> NAME/KEY: exon <222> LOCATION: (92254)..(94932) <220> FEATURE:  <221> NAME/KEY: exon <222> LOCATION: (97360)..(98436) <400> SEQUENCE: 1 attcgaaacc cctcgcggcc gcgaccgaac ggcgaaaaaa atttcacctc aatagaagcg     60 aacgggcgct ggcgagattc ggccaaaatc accggataaa tttccaccgc gaaatggcgc    120 gtttggcccc ctgccagcac gggccctgcg tacgtgcaca gcacccctgc cccgcggcaa    180 tcgctcccct actttctgtt ttgcttcctt ggtatttctc cgcatcctgt tttcctgaaa    240 acttagacgc ggtgttgcta agcagctcct cggcatccgg tatatgcagg caagcaccca    300 gcgcgagcgc cattaaaagc gcggcagcgc acagcagcca cactccctac ctcgctccgc    360 ttcttccccg cctctctcca ctgtcctcta ctgctacccc ttcccattga ccctttctgc    420 tcctctacag ggacgtccac tcattggttc tttgttctct cttacagggt tcgcagtgcg    480 cttgtctctg acgggaccgc ccgccgttgg atttcgcgag gctccgcaga ggagactgat    540 tggggaatga ttggtaagct tgtgccaatt ccattcctct ttctgtctcc accgctgtgc    600 cattcgcctc tttccagtgg gtctcttcca ggtgctagga cgcgacccct gcctaagcga    660 ggctccgcac cggagactca ggaatggtca ttccattcct atctccctct ccgatgcggc    720 gcccgccgta ccctaccgtt ccgctttctg gttgcgcaaa ttgctctggc ggtgagcggc    780 ccaaatcctc ggctgaattg caggctgcaa tgacttcctg atttaattaa tttatccggt    840 gattttggcc gaatctcgcc agcgcccgtt cgcttctatt gaggtgaaat ttttttcgcc    900 gttcggtcgc ggccgcgagg ggtttcgaat aggatcagag accgcctcca atccctctcg    960 cccccgcgct tggctctgtg ccgcacaccc caccctcctc gccgagcggc tgccaccatt   1020 ccctctctcc gatagatccg atgcctctgg gggtccgtgc caggccggtc tgatctgtgc   1080 tttaacgctg acagccgaga atgtgcacga agccgccggg ttggcatata cctccgcggt   1140 ccgctgcgcc ttccccccca ccccccttca gccgccacgc tatccaagct ctccgaggta   1200 tctgggtgcc gccagggttc ctccgcatgt actgagatcc ctgaggggcc tacccagatc   1260 ttcggagcct tggcttaccg acggcgcgcc ctgctctcca gcagctttta agccccctac   1320 ttgctgagag gtggcccgcg cttgctttcc gctcaactgc cttttagctc cacagctcta   1380 aaccccggtt ctacgatact agccgcggaa ggctgtgcga taggagccga gtacacttaa   1440 gtttatccgc tcctatgcag tagcctccgc aagctagcgt gtagatactc catcgatgcg   1500 ggacagcaaa ggtgcaggac ggcaggacgg caacatttga ctggcaggta gtaccgttgt   1560 ggaaaagccg tactgcccgc tcccgccgga gaccaagtta ctgcgtttag agctatcttg   1620 tctctgggtg ggttcggaga gtgcgggatt ttcgggcatc tagtctctgg ccagtcgagg   1680 atggtccatc cagctgtcgt gtatgtatag cgagcaatga ccgtgtggtt ccctcatcac   1740 ggagtgctcg ttatgcatag atgcctgcgg gctaggtcac acttgctggg cagcggctaa   1800 actggctagc ctgcaaatag ccctccgacc tctcaggcga ccctcactta gggtcattgt   1860 cggagctttt cggcacaaag tacaagccca ttcactcgcc ctctgctaaa ctcaccagtt   1920 tgctagccag taacggttag tttaacagtg ggcgcatgaa agggctacac tgtaatccat   1980 acattccttt ttaggatttg catattcctt tgcctgtgat accgttaccg ccgaaggtag   2040 gtctagggtg tggatacttc ttgacctctc tttcggccgt cacagtatct tctggatatc   2100 ctcctcagcc atctcgacgg cgggcttccc agcggccggt attcctaacc atgctatctg   2160 taggagcttt gctccatagt tatgggtgcg gaatgtgtca gtatttagct attgttatat   2220 ttactgggca gggctcgttt atttgcccaa attgagcagc tcggactaca atacgaccta   2280 caaagagctg acacagcaca gtctctaaca gctgttcctg gagtcattta aacagaacga   2340 taaattcgaa ctaaatcggg acagtccctt cgcttgcacg gagtccaggt aacactgcgg   2400 atctgacaat aggattggtg agaacaagtg aagatgccag ctcccgacac gaactagtga   2460 cctagtccag acgaaaatgc ttaagcttgt ccttgattgg acaagcacga tgaaaacgaa   2520 accctgcata gatattggcg agctggctct ttacacactc tgcggatgta gcttagtggg   2580 cgggtatcgg gtttcgccgc actgccgaac gctgctttgc ttcttgctct atctatttta   2640 tagccgggcg cttttaaaga atggacttcg agctttgcat gttcttaatt actttaattc   2700 tagcaattct tttattttta gctatattgt atctacagtg ggtccgtcat cgtagagaag   2760 ccgcagagag gctgctattt ctgcttttaa tacacaacat tcagaacgat gcgggcttaa   2820 gacggaaccg cgcggcacaa ggagcataac tgcggggtca gtgtttgttg ctgcgtggtt   2880 gtaattctct ttttggcact gcgaggctag gtttgttgcc gcgcgattgc agggtttttc   2940 tttgtgagac tggtgatggg gaaaagcttg attgtgcaat agtaaaagtg acttaggtac   3000 ttcatatact gggctgtggc atgtaggcac gtccaagctc ggctctgaat tctaagcttg   3060 ctttgtcgaa ccagttttgt tttctttttg gggggagggt agcacactct gcccgagtct   3120 cggcattgac ttaacagtga tgtgaaaccc ggaagatcga gcatgaacta atagcattaa   3180 agaattgtta tccgaggaat aatcgtggac gcgaatttac tcgaccgcta aaatctttct   3240 tctactgagc tggatacgtg aaatttggtg agtataacct ctcgggatac atagctttta   3300 aatacggggc gtgcaatatt aaattctgca ctcggggctg caatggagcg cggagcttta   3360 tttgacaaga ccgccaattg caaggactgg gtctcgatcg ggactactgt gtggggcgcg   3420 atcgatgcag atgacgggga cgacttagtc tgggattatg aaaatagccc atatccaagc   3480 atagtttcct cactattccc gggggaagaa acggactcgg caatttgtaa ctctgttgtt   3540 gccgcaaacc cctgtagcat acctcctggg cggcagcgtt tggcatggcc atgctgctgt   3600 tttcgtcggc cagacagctt ctccgtcccg cgcgtggaag ttaatgctcg ccttgttgcc   3660 gcggttgcac tgataatttt ctcattgctt gtagtgatct gtgttgcgtc atattggggg   3720 taacatgtct tcagaggaca catcgggatt cctaacgccc cccgcaagtg atgacgacac   3780 tgacccttcc gagccaccac caaatttatg ggatcctcac caggacgatt ttccgaggga   3840 cgctgattcc ccaaacccac ttttctaccc ctgggatgac tctgtgaata atactgggga   3900 tacgggcagt aacgaagatg actatgtaga tatgggaggg gtaggtggat ccgaagacta   3960 tgaagacctc ggtacgggcg gggactctga ctatgacaat gtatctacag cgaccggcgg   4020 gacgtggttt ccttccctta cttcttggtc atcagaggac cacggcccaa cttctccgga   4080 aaaccctatg caacaacttc aagtaacaat tcagcaggat tcagatccac agcaggaacc   4140 cgatccccag caagttcccg gtctccagca ggaacctgac ccccagcaag atccacgaga   4200 gcctcgtgat cctcctccct atagtccgcc cccagaggac ccttttgggc tctcgccatt   4260 tactagtggg atgggcgggt ttgggccacc gccacggtgg ccccagccac cctcgtatga   4320 tgaggcaatg ggggatggac cttttactac gactgggggt cggcgacctt gctcacgcag   4380 gcgcggtcgt cgccggtctc gaggtcgatc tcggcgacgc aattggtgtg cagcaaagtt   4440 gtgctcagag gcatgtttgt ggcatctctt ttgggttgga gtggccttaa tgtgttggtg   4500 gctcttgtat cttattttgc gcattgtctg gggacagact ccgggataag gaaggttgta   4560 tccgcatcca gtactcctca ataaaagcgt ggtggtgcta cacgatgtct gttaatttta   4620 caactccatt ttacaggtga tctagagaga cgctgagtgg cacttgtccc gacgggacc    4679 atg cag tcg aac agc agc gat gag gcc cag tgt gat gat gtc gag gag     4727 Met Gln Ser Asn Ser Ser Asp Glu Ala Gln Cys Asp Asp Val Glu Glu          1               5                   10                  15               gga tgg tcg tcc ata gct cca ggt gat gca ctg gat aca gat ttc att     4775 Gly Trp Ser Ser Ile Ala Pro Gly Asp Ala Leu Asp Thr Asp Phe Ile                      20                  25                  30                   cca ggg cct tgt gcc acg tcc ata cat ggt ata tcc aag gca gtt tat     4823 Pro Gly Pro Cys Ala Thr Ser Ile His Gly Ile Ser Lys Ala Val Tyr                  35                  40                  45                       ttt ttt ctg tgt gga gtt aat ctg gag gaa tgt agt aca ctc cca cag     4871 Phe Phe Leu Cys Gly Val Asn Leu Glu Glu Cys Ser Thr Leu Pro Gln              50                  55                  60                           cat gtc caa tct cac cca tat gga cat cct gag ctg aaa tca ggc aaa     4919 His Val Gln Ser His Pro Tyr Gly His Pro Glu Leu Lys Ser Gly Lys          65                  70                  75                  80           tgg tac aag agg ttt tgc tcc ggg cta ggc gaa att gga gat aca agc     4967 Trp Tyr Lys Arg Phe Cys Ser Gly Leu Gly Glu Ile Gly Asp Thr Ser                          85                  90                  95               cag tgt cag ctg aca cga cta tgc tgc act tcc gga atg ccg gca cag     5015 Gln Cys Gln Leu Thr Arg Leu Cys Cys Thr Ser Gly Met Pro Ala Gln                      100                 105                 110                  att ttt ggg cct tcg aga ttc agg tct ctg caa cag aag cca acc cat     5063 Ile Phe Gly Pro Ser Arg Phe Arg Ser Leu Gln Gln Lys Pro Thr His                  115                 120                 125                      atg cgg gcc caa gat ttg ctc act agg cct tgc cat ata cta gag ttc     5111 Met Arg Ala Gln Asp Leu Leu Thr Arg Pro Cys His Ile Leu Glu Phe              130                 135                 140                          gat gtt ggc gct gac cta atc aat ctt ttc ttg tat atg gaa cca tgt     5159 Asp Val Gly Ala Asp Leu Ile Asn Leu Phe Leu Tyr Met Glu Pro Cys          145                 150                 155                 160          tca ggg aat cga tat tgc gta cat tta gga tac cat aaa act aat gcc     5207 Ser Gly Asn Arg Tyr Cys Val His Leu Gly Tyr His Lys Thr Asn Ala                          165                 170                 175              atg cgt gtt ttg agc ggt ggt ggg att cta tgg ggc aga ctt ccg tgg     5255 Met Arg Val Leu Ser Gly Gly Gly Ile Leu Trp Gly Arg Leu Pro Trp                      180                 185                 190                  aag gac aac acc gag gag cac ggg tac tcg ttg cct atg cga gta ttt     5303 Lys Asp Asn Thr Glu Glu His Gly Tyr Ser Leu Pro Met Arg Val Phe                  195                 200                 205                      ggg atc aaa ttg ccc cat aaa gtt tat gtg gca tgt cgc tgc cct gca     5351 Gly Ile Lys Leu Pro His Lys Val Tyr Val Ala Cys Arg Cys Pro Ala              210                 215                 220                          act cgg acg gaa cta tta ttt ggt gag ggg ggg gta gga ttc aac gcg     5399 Thr Arg Thr Glu Leu Leu Phe Gly Glu Gly Gly Val Gly Phe Asn Ala          225                 230                 235                 240          gaa aac ttt aaa cag tgc gga cgg ttg aaa aaa gag tgt gaa tgt ctg     5447 Glu Asn Phe Lys Gln Cys Gly Arg Leu Lys Lys Glu Cys Glu Cys Leu                          245                 250                 255              cag aag gct tgt ttt act gca caa acg gtg tta ggt gcg gca tat aag     5495 Gln Lys Ala Cys Phe Thr Ala Gln Thr Val Leu Gly Ala Ala Tyr Lys                      260                 265                 270                  ttt act gta tac tcg agc aag gga cga ggt caa gaa att ctg cta tat     5543 Phe Thr Val Tyr Ser Ser Lys Gly Arg Gly Gln Glu Ile Leu Leu Tyr                  275                 280                 285                      tca gga ccc atg aat gct aca acg gta atg cct gta gta ctg ggt atg     5591 Ser Gly Pro Met Asn Ala Thr Thr Val Met Pro Val Val Leu Gly Met              290                 295                 300                          tta agt aga gaa ccc cac agg tgt gca ggt aca ctc ata ctg tcc agg     5639 Leu Ser Arg Glu Pro His Arg Cys Ala Gly Thr Leu Ile Leu Ser Arg          305                 310                 315                 320          tcc tct gga aat tgc cgt gga ttt cat gag acc caa cac gat att ccc     5687 Ser Ser Gly Asn Cys Arg Gly Phe His Glu Thr Gln His Asp Ile Pro                          325                 330                 335              act aac ccg ggt ctg tat cct ctg tgt aat cat gag cac cct tac tat     5735 Thr Asn Pro Gly Leu Tyr Pro Leu Cys Asn His Glu His Pro Tyr Tyr                      340                 345                 350                  gtg aca gtt aca gat gta tgc ggc aac tgt tgt tca tgg ctt gag cgg     5783 Val Thr Val Thr Asp Val Cys Gly Asn Cys Cys Ser Trp Leu Glu Arg                  355                 360                 365                      gtt ttt ggg aga gta gct gcc cct gct ggt cta agc tcc gta tct gta     5831 Val Phe Gly Arg Val Ala Ala Pro Ala Gly Leu Ser Ser Val Ser Val              370                 375                 380                          tcc att aaa ggc tcc acc cac agc ggg act gac gtg aca gaa gaa cgt     5879 Ser Ile Lys Gly Ser Thr His Ser Gly Thr Asp Val Thr Glu Glu Arg          385                 390                 395                 400          gaa gag gac tca ggg aca cag caa acc tcc cac gac aaa ttg ccg gag     5927 Glu Glu Asp Ser Gly Thr Gln Gln Thr Ser His Asp Lys Leu Pro Glu                          405                 410                 415              cgc aac cgc atg gga gat caa aat tcg aat ttg cgg gga aga gat caa     5975 Arg Asn Arg Met Gly Asp Gln Asn Ser Asn Leu Arg Gly Arg Asp Gln                      420                 425                 430                  tat tgg ccg cct gcc cca cac cgt agt cat tgt cac tcg gat ttt ata     6023 Tyr Trp Pro Pro Ala Pro His Arg Ser His Cys His Ser Asp Phe Ile                  435                 440                 445                      ttc gat gaa cct gag cca gaa agt ggg gaa gac gtg cat aac atg cat     6071 Phe Asp Glu Pro Glu Pro Glu Ser Gly Glu Asp Val His Asn Met His              450                 455                 460                          cct cca cga ggt gca gat gag caa aca gcc gct tct gtg tca gcg cta     6119 Pro Pro Arg Gly Ala Asp Glu Gln Thr Ala Ala Ser Val Ser Ala Leu          465                 470                 475                 480          atg caa agt cta gca caa gca ttg gtg agt gca caa gct att agc agc     6167 Met Gln Ser Leu Ala Gln Ala Leu Val Ser Ala Gln Ala Ile Ser Ser                          485                 490                 495              atg gtc tct ggc tct gct tcc tca gtg ggc gta gaa gta gac tgt ggg     6215 Met Val Ser Gly Ser Ala Ser Ser Val Gly Val Glu Val Asp Cys Gly                      500                 505                 510                  tac agt cag act cat att aca gag ggg ccg ggg agg gaa caa ttc ggt     6263 Tyr Ser Gln Thr His Ile Thr Glu Gly Pro Gly Arg Glu Gln Phe Gly                  515                 520                 525                      aga gtc cca gaa aga ggg cca gag tat cct caa gat tac tgt gat ata     6311 Arg Val Pro Glu Arg Gly Pro Glu Tyr Pro Gln Asp Tyr Cys Asp Ile              530                 535                 540                          tat ggt cct gta agt aat ggg cct gct gga tac aga gca gga cca cca     6359 Tyr Gly Pro Val Ser Asn Gly Pro Ala Gly Tyr Arg Ala Gly Pro Pro          545                 550                 555                 560          gat gct cct agt ata caa gat agg acc ttc cca tgc ggc aga aga tgc     6407 Asp Ala Pro Ser Ile Gln Asp Arg Thr Phe Pro Cys Gly Arg Arg Cys                          565                 570                 575              gac gaa gca tgg ctt gcc tta gaa gta ggg aat atg cct tgg att tct     6455 Asp Glu Ala Trp Leu Ala Leu Glu Val Gly Asn Met Pro Trp Ile Ser                      580                 585                 590                  tct ggt tca cat agt cca cct tct cag tat cat aac cct tat ggt tca     6503 Ser Gly Ser His Ser Pro Pro Ser Gln Tyr His Asn Pro Tyr Gly Ser                  595                 600                 605                      cat agt cca cct tcc cag tct cat aac cct tat ggt aca tat agt ccg     6551 His Ser Pro Pro Ser Gln Ser His Asn Pro Tyr Gly Thr Tyr Ser Pro              610                 615                 620                          cct tct cag tct cat aac cct tat ggc tca tat agt ccg cct tcc cag     6599 Pro Ser Gln Ser His Asn Pro Tyr Gly Ser Tyr Ser Pro Pro Ser Gln          625                 630                 635                 640          tat cat aac cct tgt ggt aca tat agt ccg cct tct cag tct cgt aag     6647 Tyr His Asn Pro Cys Gly Thr Tyr Ser Pro Pro Ser Gln Ser Arg Lys                          645                 650                 655              cat gac tat tca cct cca tat ccg ata ctc aaa cca aag cct cga tta     6695 His Asp Tyr Ser Pro Pro Tyr Pro Ile Leu Lys Pro Lys Pro Arg Leu                      660                 665                 670                  ccc cca ggc ttt gaa aat act gct ggg atg tgg cct cga tgt ccc cct     6743 Pro Pro Gly Phe Glu Asn Thr Ala Gly Met Trp Pro Arg Cys Pro Pro                  675                 680                 685                      ggg ttt gag ggg cgt cca tac aaa tct ggg ggc atg ggt aac ttt cct     6791 Gly Phe Glu Gly Arg Pro Tyr Lys Ser Gly Gly Met Gly Asn Phe Pro              690                 695                 700                          gga agt gca tgg acg gta ata gat agg ggg tct aac caa tgg cca gca     6839 Gly Ser Ala Trp Thr Val Ile Asp Arg Gly Ser Asn Gln Trp Pro Ala          705                 710                 715                 720          gac gtg cgg ggg cca ttc tca gat caa cga tgg gcc ccc aca gag cat     6887 Asp Val Arg Gly Pro Phe Ser Asp Gln Arg Trp Ala Pro Thr Glu His                          725                 730                 735              gaa acg cga cgt ttt tgc ggg tat tac agc tga gctctcatca tacccataac   6940 Glu Thr Arg Arg Phe Cys Gly Tyr Tyr Ser                                              740                 745                                      tccactcata acccaaggcc cataaatcca taactcataa cataaattca tactttccgg   7000 tcgtccaggg caccacgtca tcaacaagga ttgcagataa ataaaaatgc tccacgttgt   7060 cggtgtccgt tgtattgtat tctttattat acctccgtaa ttttcgagag tcggggaaca   7120 ttctaaaaat tttaaccgtg caatactaca gtgtatttac aaggccggat tgcaacagtg   7180 aactcattac atcattgagc tcgcggcgcc atctgctgac cagtccacag agatggcaat   7240 cttcagaaac gtaggatggc accaattcca atacaatacc gccatctgtc gataggtgta   7300 tagaactgtc aaaacaagtc gcaagagaaa aatttcccta ctgtatactg gcggcttagc   7360 agctgcgcac aaaccactct gcattcctct ttgcggcaca catttgcgtg ctgcgccaga   7420 acgagtggga tttttttaga acaggtccca ggatagtaca tgtcccacaa tgttctggcc   7480 gggtctattg ctttatgatt catgactatg gcctctggtc gcggatacac aattcttgag   7540 aaccggtcga agaaggtcag taataaagtt aaaggacatt ttgcgctact cagcgatagc   7600 tcctgagatc tagtggtatc tcttagttga ctgccaatgc tagagagata acacggcagg   7660 attggcccca gatgcatggc tagagattga catgcgcagt agatgttaga gaagatagga   7720 tcgtgggggt aaatcctttc atcttcgaac tgatgccaaa gcatccatac aagtgtctca   7780 tcgcatgcaa aaagtagctc ttcaaatgag cagttcgcca aatatacagc tcgtgaaatt   7840 tttgccaacc tggctatatc cggacgcgat gtccagcggc ctttcagtga agctgcgcgc   7900 ccacaaaact gcttccacga agtgaatgca gcatctgctg caaggtcaga tgatcccgaa   7960 gacaaaaatg ctggaaagca gattcctcta tcacgatcga tatcatcaca atcatcatca   8020 tccactgccc ggtttaccat gtctaaaaga catttctgat tttctaatct taactcttca   8080 gtaatgcact ttccgagacc gccaaatgca gttgcggcct tttcaaaata ttgggccggt   8140 gttacgtttc gcaactcctt cgtttcggtc cgtgatgacg ttgggcatcg gacaaagtct   8200 ctccaaatcg gtcttcgaag ttcatcccga tttctttccc aagacctgcg cgaatgcttc   8260 aacgaaacag taaagatagg cgccctatat cgcttttctg gtgtacctgc acggcgcctg   8320 ggtctagggg ggatgcctct tcggacttgg atatgcgcac ggcctctcat aaacttattt   8380 cgagggctac tgacccgcct tatggaagac cgtacgctca tacctgactg gttctcgtaa   8440 cgggaacgag gctcgccatt cgaatagcga cggcgccccc tgtcacgtga atcacggact   8500 cgtttccagc taccgcgccc agatgaccgg gatctggagc tactttctgt agaatgagat   8560 ctgcggcgaa tacaatgtgc ttcgtggcgg gattctgacc tcgagcgtga acggccatcc   8620 aggcgatctt ttgtcctttc cgttgctgat ctcgcttcgc tatgagtact tgtattggaa   8680 gatgatctgg aacgtgtctc acgcctatct ttatttattc cagatttctc cggtctcccc   8740 atcgcagtca gtgggttgat gtaccgcgtg cacgtcaaaa aaaatgaaac cgcacacaca   8800 acggttgaga cttctacgga ctcagaacag gtgtcaagct cggagcaggt gctgagaggt   8860 aagctgacag taatctggca cgctgtttgc gagctaatcc acttggcttt tgaatggtct   8920 gggccactcc cagtatacgt cataacacat acactggaac ccacaaacta caattgcggt   8980 ccagtagttg gtgcgaaata ttcacgcaga cataataatc tgcgagaact tctgcggatc   9040 cgacatgtaa cttaatcccg taatgtagtg cggcataccg tctaaaccgc aaacatccgc   9100 ttagtagaac acgccctaaa atcacccacg agtatacttt gtacattctg accgccagta   9160 gttactcctt tcaaacaatg atactcagcc gttagaacta gggctgtctt caaatggacc   9220 aaattcagac acaataccgc acaacgtgtt ttaacatttt attgccgttc aaggcccgaa   9280 caatttgttt tgtatcttct gttcgtattt aaatgcaatt attacaatgc tcgcaatcgc   9340 agccacgcac aatgcacgca agactaagct cgaagcaata ttggcaaggc atgaagtcaa   9400 gatactggga ggtttcgatg cggagacttt agtctttgcc ggagtcgtgt aagggatcag   9460 tgataagagt tctagaaccg ctgagacaat tacatatacc caagcactaa tttttacgtg   9520 ttcatgtaaa atatatgcac acggatcata gcaaatccgc aatgtcatgg tggacataat   9580 aagcgatgct aaatataaac cgatctcgag tgttttcagt agtaagaccc ctggattaca   9640 gacgcaaaat cctagagggt ctgctacgta gttcaagctt ttgcaatctc ggagatgttc   9700 aaattccctc aaaaaccgtt gcatctttgt gtatggcacc cgtaatatca gcctcgaagt   9760 aaacgctgtc cagtagttaa gatgatatgc atccatcgca atgcgttctc gcggcagaca   9820 tagcccgaac attcgcctct ggcgacgggt cagcaagtaa catatgtaca ataccgtcga   9880 aatgaatgca actctgatct gggaaaacca tatgtaaagc agacagtctg ccacttccat   9940 gacgaatact gttctttggc cggtgggatc aagacgagaa atgagtacag tctcattttc  10000 aaaataagct cctttaacac atctcgtctg gtggttctcg tagttgttta gacgctgtgt  10060 atattttgtg aggacataaa ctagtgaaga attaaatgta gcggggggtt ggtgaacagg  10120 attgaattct tgctgtccgg gcaggagcca tttaatattt gcgagtacgt acaagcagtc  10180 gtcctttgag tcagtggaaa ttttagagca tacataccat accaagaaga ctatatgtat  10240 cgttccggtt aaaattactg cccattttag acattctggt cgcagcattc tttggtcaga  10300 cgcactgtaa actgagtttg cagcgtgttg cctccgcact tagtcgcaaa gcatttggtc  10360 tgaaatgccg cgtagggatt gccctgtgag ttaattgcaa ccgctacaga agtaatgacg  10420 gtctgggcag cggcacttcg atgcgaatgt ttaaggcagg taaaagttgc gcgccctccg  10480 caccctcgac gatttcgtaa tagtgatgcg gtaatgcgcg ttttgagcac ccgcacgtct  10540 ccaaaactaa tgtcgctgta ttcgtgcgcc aacgatggaa aatgctcttt tatgtaattt  10600 gttatggccg gcagaacata tgtcgtgagc acaaagactc catcgatcgt atccaccccg  10660 gctcctgtcc tgtcttcgca cggtgctaaa ttgacacgac tgaatgcttc cgcgaggcta  10720 agagacacag ccgtttgggg caagttgcta gagggttttg aaactccccc tataacgtca  10780 ggaggacacg cgattcgagt gacgataata tttggggtgg gattgtctgg acggaaagca  10840 tgaaaatgga aattattagg gtctttgtgc gctgccgcga aagctacttt atctgcacag  10900 tcagggggga aaattataaa cggcaggagt ctcccagaca tgaatccgga ttcagaaacc  10960 ttggcaaaga aaggtagccg gagacttcgt cctggcgagt agattccagt gtcaataaag  11020 tcatagtctc tgataacatc gtctaaacat tcaatgattt cttcctgcag taaaattacg  11080 tgctgagcta gcttcgcgat agtttttact gtctctagac caaacaaagc atatggggat  11140 gggattggga cgcagactct gaacccgagc ttttctttgc atttgcataa ggttgaattg  11200 gcacgatccc gaaatacatt taacgcatcc tgtaagtctt gaacagagac tatggctcca  11260 tgttcactcg catgctcatc acaccatgac atttcttcta cccagccgct atcgcagagg  11320 tacatgttct cttcgtcttc tatgtcatca gaacatgtat tctccaaaaa atcaatatcc  11380 actgaggaat attgtgcgtc atggctctcc ggggacgagc agtgactttt gtaaaagtac  11440 acgggatagg tgtcatgact gattgatgtc tccggaaaca ataaggctat taattggatc  11500 aatgccacgc gcacggcccg catcgcttta tgtaaggtca acatcgttat ggggccgtga  11560 tctagcttga ggggtatgtc gatatccagt atcagggatt gaattgctag agattcgtta  11620 aggacttcat tcctattcag gtacatttgt gacagaccgc tgcaagatgc ccgaatttta  11680 tttttcgaac tagttcccca tgcggtttcg ttcattgcac tagtccaagc aaagtctatt  11740 actggcaaca tggcctgatc tgcgaggggg gcatcactgc gcattagtaa gcaggagtcg  11800 tgtatgctgg tgtcagatct tacatcagcg gttataatcg agtcccactc atcgctggca  11860 atggctgcaa aagcctgtcc gtgtccagcc aatgttactc tgtaaacggg acatggtccg  11920 ttcctagctg ttggtggagg aatgcttgtg ggtttcagag aatggaataa gagtgtcact  11980 ggtcgctttc cgcaaataac ttgatcagat gaaacgagag aaagtaacct acttaaggga  12040 gcaaaaatga tatctcctgt cttacagtat tgcccgtcgc gctctctttt ttgtgcaagg  12100 gcgtgtggag cggaagcatc atctccttcc tcttcttcct cttcttcctc ttcttcctgt  12160 tcattgctag atacagtgct cacatttctg accagcatgt cagccaagtg tgccccgtag  12220 cgacagagag tatcggaata tagacggtct atacgcgttg atacatcaaa tgcggaacga  12280 caaaaaccaa gaaaattgcg cccctgtatt tcaccgtaag attttatagc tgcaaacttc  12340 cctgctacta cagccggcaa tttcatgact tgcatagtca catgctgtct gaagtacgaa  12400 gcaaaattac aacattccct taagctttta aaaaaatcac tttgagcacg agcagaactc  12460 ttaactggat tggtgagatt ttcagaaaga cattgcaagt gttctctaat cacgtttcgt  12520 ccatatcctt cactgtaagc taaatataca aatgagatga agtcacgagc tggaagccgc  12580 agggcatgtc tgtatttgca gataagtgct tccaattgtt ctgaatagcc cctgtcggcg  12640 ctagtacgta atctcaaatg atctctgaat agctttgcag attcgcgagt ggcatagtag  12700 ctgctgcacc agaatttaga aaacgcagag aaggacgtta ggcgcgctat agtcaggtta  12760 ctcctgttgt tgctattctc cgtttgaaat gtaatgcata gatcctttac tgcttgaagg  12820 tcaaacgtcc cagcaatacc atcgtcaggg gttgcttcta acaagtaata tctcatggct  12880 gcaacaaggg taatttctga cgggccgaat tttgatacga accaaaaggg gttcgaaaag  12940 ttcgagttgt aaagcctcct gtatgcggca atcacccttg actcatgctg aagcatcagg  13000 ccctccaggg caggtcttcc tctgggtaga gtcgcatgta tggcagaaaa aggaattttg  13060 ttggttgtat tttctgctcg gaaaattact ccgagtgcct caataatgtc cgtgtgcaat  13120 gcaaatgttt cctccaaatc catggtttcc agtaataact ttgcatcgat tggaaaccct  13180 tcggctaagg cttctctcat agttgcggcc ccttgaccga caaaacgaca cacaaacact  13240 ggtctagacc tctgcctgta cttctccgct ccggtattag caggaaggaa gaacgtgatt  13300 ttgagagggg aatctttgca aaaatcttgc gctagagcgt caacatgact aaaggccaga  13360 acataaattt caggctgcag ggcttggccg agtagaaggt gcagggagca tactactgga  13420 tacccatccg tagcatacaa cacgcgaatc tcttcgtacg aaacatcctc actaggggga  13480 gcgccgcagg gttcacaata tccagcttgg gaagtctctg tctcatttag catttcgacg  13540 agcatccagt catctgcctc atactcccat ttggcgtctg ggacacagtc gaagttcatg  13600 atagcaagga aatgtggcag ctactgaaga gcgtgttctg cggccggtct aactcagaga  13660 gtaaatacga agcccttcca actgggcggt gcctgcccgt agatgatcga aaagtacagt  13720 cggctgtgaa gcttattaaa atctttatcc ctaactgctt ggacgtcgaa gatgtcatcg  13780 gctcgaggga tgaacttaac aaactcgctc aagccaggca aattagccgc attctggcaa  13840 aatcggccgc ggctatgcag attgctaaga atatgagatg cccgcgagga gcagaggcgg  13900 ttttgcggca aacgattgtg gacaatggga cggtatttcg ttctctgtac tctgtactag  13960 cctacctata tctctcccct ggggcagaca cggatggact acttgcacaa gtaaccgctc  14020 aaaccgcaga caggacgatg atgctaggtg acatgaccgt gcttacacat gcaatgcacg  14080 tagacggaat ggatcgtgac catagctctt tggaactact gcgcatgggt ctggtaccaa  14140 attcagacct gaaagatcca atcgagctcg tgccggtaat tgataaactg cctgacaagg  14200 tactgttccc tgatcccgta ccgacactgc cagttgaaaa cccacccatg gagcgtacta  14260 gacctgcaaa gacacgtaaa gaactggttg ctgcgagtta agtgacttct cagcgcttta  14320 ataaagtatg tttaaatttt ccaatgtcag taca tta ttt gcc ggt aga tcc aaa  14375                                       Leu Phe Ala Gly Arg Ser Lys                                                          750                    ccc ccc gtc acc ccg aac cga caa cgt att gcg ttc gtc tac tac tga    14423 Pro Pro Val Thr Pro Asn Arg Gln Arg Ile Ala Phe Val Tyr Tyr                  755                 760                 765                          gtt tac tat ttt cca gcg aag ggc atc tgg ctc tcc gag aaa ggt gct    14471 Val Tyr Tyr Phe Pro Ala Lys Gly Ile Trp Leu Ser Glu Lys Gly Ala              770                 775                 780                          tct ctt ctc agt aac tat tag ctg tgc cac tcg gtc acc tgc gtg aat    14519 Ser Leu Leu Ser Asn Tyr     Leu Cys His Ser Val Thr Cys Val Asn          785                 790                     795                          act ggc cgc ttc atc tcg aat gtt agt tag cga aaa tga tat cca atc    14567 Thr Gly Arg Phe Ile Ser Asn Val Ser     Arg Lys     Tyr Pro Ile          800                 805                     810                          att atc ctg cac atg tat cgg gtc gac taa cag tcc ttt cag att cat    14615 Ile Ile Leu His Met Tyr Arg Val Asp     Gln Ser Phe Gln Ile His              815                 820                     825                      gga gga tct ccc tag tat gaa tgc gcg cca gaa agg tcc gca tgc tag    14663 Gly Gly Ser Pro     Tyr Glu Cys Ala Pro Glu Arg Ser Ala Cys                  830                     835                 840                      ttt ttg ggg aag ggt aat acg ggt aga cga ctt tgg aag aat tgt tac    14711 Phe Leu Gly Lys Gly Asn Thr Gly Arg Arg Leu Trp Lys Asn Cys Tyr                  845                 850                 855                      atc ttt agt agc tct aat atc ata gcc tcc atc tcc tgg acg ttt ggg    14759 Ile Phe Ser Ser Ser Asn Ile Ile Ala Ser Ile Ser Trp Thr Phe Gly              860                 865                 870                          ggc aaa gat ttc gtt aaa cgg aaa cag tgc ggc agg gca atc tgg atc    14807 Gly Lys Asp Phe Val Lys Arg Lys Gln Cys Gly Arg Ala Ile Trp Ile          875                 880                 885                 890          tga tcg cag ctc gac tgt ctg atc ttt aac gag cga tcg tgt agc tcc    14855 Ser Gln Leu Asp Cys Leu Ile Phe Asn Glu Arg Ser Cys Ser Ser                          895                 900                 905            gac gat gat atg gtg tcg cac ctg ggc tac aaa ctt gat gtt cga tgc    14903 Asp Asp Asp Met Val Ser His Leu Gly Tyr Lys Leu Asp Val Arg Cys                          910                 915                 920              gcc aca aaa acg att gat att ttc tgg cgt cgc tcc att cag tat aac    14951 Ala Thr Lys Thr Ile Asp Ile Phe Trp Arg Arg Ser Ile Gln Tyr Asn                      925                 930                 935                  gat gcg ttc att gca ggc tgc atc ttg ctc ctg caa ttc ttc gaa tgt    14999 Asp Ala Phe Ile Ala Gly Cys Ile Leu Leu Leu Gln Phe Phe Glu Cys                  940                 945                 950                      tct tct gta aaa cgt tgt aca tcc cat aca acc aat gtt gcc cgg aat    15047 Ser Ser Val Lys Arg Cys Thr Ser His Thr Thr Asn Val Ala Arg Asn              955                 960                 965                          agt tat ttt cgc cct taa tat atc tgg agg tat act taa aag gga act    15095 Ser Tyr Phe Arg Pro     Tyr Ile Trp Arg Tyr Thr     Lys Gly Thr          970                     975                 980                          tat aca ttt ctc gaa acc ttc acc caa ata atc atc ttc gcc ttg gaa    15143 Tyr Thr Phe Leu Glu Thr Phe Thr Gln Ile Ile Ile Phe Ala Leu Glu              985                 990                 995                          aga  atc tgc ggt ttc taa gag  tag gcg cgc gta tcc cgt  ccc aca     15188 Arg  Ile Cys Gly Phe     Glu      Ala Arg Val Ser Arg  Pro Thr           1000                     1005                     1010                   ctt gct aag  cat gga atc gtt agc  caa ctt tat aac att  tag ccg     15233 Leu Ala Lys  His Gly Ile Val Ser  Gln Leu Tyr Asn Ile      Pro                   1015                 1020                 1025                   agc cct tac agt  gcc tgg tgg aaa tat  aac att tcc gtc tct  tgt     15278 Ser Pro Tyr Ser  Ala Trp Trp Lys Tyr  Asn Ile Ser Val Ser  Cys                       1030                 1035                 1040               ctc tgt gcc agt  caa aac att ccc gcc  atc gtt tac aag tac  tac     15323 Leu Cys Ala Ser  Gln Asn Ile Pro Ala  Ile Val Tyr Lys Tyr  Tyr                       1045                 1050                 1055               cag tat tcc caa  taa tcc tgt ata gtc tgc  atc tac caa tcc tgg      15368 Gln Tyr Ser Gln      Ser Cys Ile Val Cys  Ile Tyr Gln Ser Trp                        1060                     1065                 1070           aat aat ttg ata aca  tgc agc atg gcc ttc  cac atc act gat aac      15413 Asn Asn Leu Ile Thr  Cys Ser Met Ala Phe  His Ile Thr Asp Asn                            1075                 1080                 1085           gat tcc ata ccc gac  tgg caa aga agt cct  aat cgc aga atc aat      15458 Asp Ser Ile Pro Asp  Trp Gln Arg Ser Pro  Asn Arg Arg Ile Asn                            1090                 1095                 1100           tct tag gac ggg aga gtc  ggt gcg tgt ttt cgc  tat tgt tgc ttc      15503 Ser     Asp Gly Arg Val  Gly Ala Cys Phe Arg  Tyr Cys Cys Phe                                1105                 1110                            aac  gtt tgt aat gac aca  ttg caa gga atc gag  gcc aaa aat gtc     15548 Asn  Val Cys Asn Asp Thr  Leu Gln Gly Ile Glu  Ala Lys Asn Val           1115                 1120                 1125                           cca  ccc aga tcc aat ctc  tat taa tgc att ttt ttc  cgt tgt cat     15593 Pro  Pro Arg Ser Asn Leu  Tyr     Cys Ile Phe Phe  Arg Cys His           1130                 1135                     1140                       ttc cat  ctc cat gcaagttgct tagacgccaa aataacaccg accctttcag       15645 Phe His  Leu His                                                             1145                                                                 gaaatccgcg tagttaaaat cggcagtaga ccggtaggat acaaatagta ccgcactgcg  15705 cgacttagag gacccgtgaa agagactgca gggcgagctc tcgcaaaaga gcaatgaggc  15765 tgccgtggga aaggagtttt atgctgttgg gagcagtggt gtatttgctg catttggcac  15825 tagtattcgc gcaaccgggt gtgacaccag gacacggaat ggagcatggg tattatggtc  15885 ttggcgctgg aaatatgctt ggagcggaaa attcgatgta tagcgcgaac atggaacact  15945 tttaccacag tgcctgctcg tcgcgcggat tttccttagt aaacggaaca gctgcttccg  16005 ttttcttttt cattagcctg gcagtggcgt tgattggcct gctggccgtg ctatataacg  16065 gttgtttcaa cagctttaaa agcagtgtca ttagttctcg atggtagcgc agtatctgct  16125 gtaaagacga aaacatcggt ggtgggacca tattagctcg aacactatgt cttactacaa  16185 agatctctcg gaggaggcga agaagtatca cgacgatact cgacggaggc gagataccac  16245 cacccgccgc cccaaaccac cccaattcga acacccctgg tctgggcgaa gaacatctcc  16305 atatctagat ttggatagag gaagcgattc tgattcttca gaaggggatt acggccatcc  16365 atcgcgcgct cactattcga gagatcatac ggcaccgcct caaggccgcc gaagcccccc  16425 gatggaaagt tttcgcaaag aaaccactcc aaaggaggag ccccctcaaa gtaaaagggg  16485 ctggaatcct gataatcatt gcgctggcct gatgcgacgt ttaactatct ctaagggatt  16545 tggtcctagt gctacaccct caggggatga agacccatgg catacttcta ctattccagc  16605 aaatcgttcg gccttcgtgc aagccgtctc tgtgacggca atggcccagg cagaattggc  16665 agcgagagag gtgtgggacg taacaaaacc acgcacgaat agggagttga gggatatggt  16725 gagagagcta gaaatcacta taattatcaa tccgggtgaa tctttatggt ctgttgccac  16785 ttcggtggca agagcaatca aagaaggaac cccaataacc cacgagctat tgcagaaaag  16845 accatcaaag cctccaaccc gccgcaaaac tgaagatgga gccagaaaga gttcttcaag  16905 gccttctcaa cccaagcctg agcatttccc ccctccaaga agaaagacat ccgaacgaaa  16965 gtactagttt cgagagtctt tgcattactg tgttcctcgc catatttgta acaataaagt  17025 atacttatga ttggtaacat gcgtgtattg tcatgtgttc atagactata aaagcgccgt  17085 gatttacatt tctgcgcaca ctgcgcagaa ccatggaaga agaatcttcc actggagcct  17145 ttgcactgta tgaagtcgaa gacttcatcg aggaagtaga atctggggct ggggggaggt  17205 gcagcgattt tgaggatgat gcgactgacc taattgaatt agaagatctc tattttgatt  17265 ggttacggaa tccaggtccc cccgcgctgg tcaatgcgcc aaagtacgtt aggccttcaa  17325 aatgttcagc atcgtttctc gcaaaaaacg cgctaacgga gctgcaattt gccgactggg  17385 acaaaataca gcacgtgttg aagacgataa acattgatat tttctcgtgt gtaccagtgg  17445 gagaaaatgg tctatgcaat atggattggt tttccctcga tacagaaaaa atatggacgt  17505 taatggattc aggaacacct aatgttagcc caaatttgct gataacacct ggcccgccac  17565 ccgaatgcat taaaactcca gaagaagctc cagcttattt tgaagcaatc tcgaattact  17625 ttgaatccgt gttagccgcc agagaagccg aatatgcaaa attactggcg gagtacgcga  17685 agtcgctcgc taaatacatc agacataaat cacaaaaagt agggcgcggt ctgcgagccc  17745 tcgaactgga cacagaggag gccaaaaaac gatttaccgt tctaatgaaa tccgaatact  17805 acaaagggat cgaaaaactt ctaattatat ttctggttca tttagtgctt aacgcgtgta  17865 gggatgttgc tcggataata tgggtccaac agagggactc ctcgtctgta ttgcagaagg  17925 taacgtatag gtgggaaagc caacagctac actgtatttt tcaaccatta cttgtttctc  17985 cgggtccagt ttttttggac aacgacccct tgcctttaca gactttgcag catgtaaact  18045 acgtacgata tcttttagga gttccggtgc taagattctc catgatagaa gaatccccca  18105 gtgcacaata tatcccttat gacaaaactg caccttctgc atatgatgtt ttagcgacta  18165 ttgcacgtac ttgcctaact tccagcggct gttcgcgtgc ttctcacgac catacctacg  18225 cacgcgataa cgaaaaacca aactactcct cctctcgaat cgtcttatta gctgattccg  18285 cccttgatac acctatgccc taaacatgac cgcccaggct ataaaacaga tggactggcc  18345 agacaacatc attgcagcga tggctgaagg cgatgggttt gaagagcgtc tccgcgctaa  18405 agcgcggtgc catcagatga agaggactcc gattggactc ctccaagtaa tccttgacgg  18465 ggcaaggaaa attgatccga aacggatttt agataattct tccccgagcg acttagctgt  18525 gttagctgag cgagagctac catcctccct gctaggaccg gcgcgcgaag aatacttaaa  18585 atttggggat aaaaaactgc cagacaactt tatctcacgc actgtctcca atgacaccac  18645 ggatgagtat agaagaaatc ttgacgaagc gtttaaactc tttattgcac gcagaggggt  18705 cactgaaaaa tgcgttgccc gaatgctcag acatctctat gccaagcggt tgtccgtggt  18765 tttggatctc gatgtcccat ttggtgaaaa aataaacaaa gcgctttccg ccgatgatca  18825 agattttgta ctgaaactgt actactgggt tttcccggac aaggtctctg cgcctagact  18885 taaggaacca gaaatgcaag cttacctggc ttcagtaacc tatttgtcgt gcacctttaa  18945 gttcattgaa ggatacgctt attatatgcg cccccctggg ccagaactcc attcaacaga  19005 aacgaccgct cgtctagcag ccctccttgt atatgtgaga agcatgtact gcaaactggc  19065 gtccttattt gacgcaatcg atgccgctgt gctgcgctct aaatttaata ggggagcttc  19125 ggctcaagct ctgactggaa tttatggata cacgcgaaac gggaagggaa ctataatgtc  19185 gcggtattct tcggaggcca tgagtgatga aatcatgtct gtgtgtcgca ctggttcgga  19245 cactttgata gaatctttac gcaaagctct ggggagattg accgacctta ttgctaggtg  19305 ggagctcatt tcggtagaga agaaaaggga cgcaaaagag gccgtgacgg cagcgttagc  19365 attaagtgga ctagttagcg gacatttggg atacttgcta aacctaatct caatggcata  19425 cgttttttgg ctccctgggc attatttaaa tatgcggtta atattggcgc tgagttccag  19485 ggaactccta agccatcttt tggctacaaa tccttcttca ttgagggaag tagccagctg  19545 cccgaaaata cttttctacc aatctgtaga agaatggaga ggacaggaaa gaggaacaca  19605 gatttggttg gattatgtta ctgtaagggc atggttggat tatatcgaca ctgggatccc  19665 tactattcgc tccgaggaaa caaatgcttc cataaatgaa atttccgagt atctggaaca  19725 gcgacgctcg gccagtccta ctgaatcctt tttatcttca gatggaccac caaactcaag  19785 cgcagcatcc ctgcccccta cacaaggctc aatgcaaaca caaaacagga gacgcgaacg  19845 acgcagtaac agtgtcgtta tccgaaagcc ctcccgctct gcgcattcaa agagtaatac  19905 cattcccgtg tttcaggaaa cgaccatttc ttattcggca ccgaattccc cggcggatca  19965 ttacgtagta atgaattaag cctgtataac tgtacagtaa atgcatcgcc aatttttata  20025 tttacgctct gaataaataa tgacattaca ttgacgtaag atgtacataa ttcgtattta  20085 tttactttcc tcgtagactc cgaaagacag ggcaactctc aatgccttaa tataccgcaa  20145 ggcaggcgaa atagcagggg acatgtagca tattactccg gcggttccag aacattgtag  20205 attctcgact agggccacgg acattttttg cgtgcttaga gatgcggtgg agtcatcatg  20265 tatagaattt atgaagtaaa cgttggcgca tttgccgcca tcatccgccc accatagtcc  20325 ttgaggtgaa gtgctcccgg accaataaaa taaccggtct aaaatagtga ggaagccgat  20385 tgcttttgaa gatgaggcaa ttttcccccc gagggcacgg caagaaagtt tagcattttc  20445 atacgtgtct tgaggcggac cacccccggc ccgataacat gaatccagat gttgaatcca  20505 gtcaggcgga cacaatccgc cttcccaggt gctgttcggc agtgcaaatg ccatgacagt  20565 gcttattaca aacataaaaa tcagtaatcc taggaaaatg ccaagcagga ttagaagaat  20625 cactttacac gccttccaat attgtccgcc tgtattttcg gggcgcattg aatgaagctc  20685 tccggaagga tggggggaat aggtacttgt cgtagaggat gaagtagagg aacaaacgct  20745 tggctcatct tccaaaatag ccctgacttc gaagggaagt tctgccatca ttttttccaa  20805 atggcttcgt tgatcttcag atggggtttg tgaaattata ggggggttca ggtagcttcg  20865 ctgatcttca gatgagggct gcaaaattac tggaagggac tggcctgctg ctatttcagg  20925 catatctaga aaatccactt gggccgctag acgaagcgag taacgagggc gctgcacgct  20985 cgaattttaa aaagaacaaa tgtcatttct gcgtatagtt tcggccccgt tatggcgtta  21045 acgtcaatat ttcacgtatt atgttattga aagtggtgtc aaggtcatca catggtgacg  21105 ctattgctta attgcgcgaa aaatgtgcgg gcgcgagaaa ctggttcata gcactcggtg  21165 cgaacgttac gtatgcagtt accgcccata tcatgtaaat caggtcacgg gactggattc  21225 gtaacctatg attggtgcgg ggaggtctag aacaaacatt cgcacctcag tattaaaaat  21285 ataatataga tatactatat ttttaatact gaggtgcgaa tgtttgttct agacctcccc  21345 gcaccaatca taggttacga atccagtccc gtgacctgat ttacatgata tgggcggtaa  21405 ctgcatacgt aacgttcgca ccgcgatttc gagtaaccgg gagcataaat accgcgtgcg  21465 tttggcactc cgtgcgcaat atgcgcccgc cactgccgac gtcagatgct gttctgatcg  21525 aaaggttaca ggcgccggaa ttctgtacaa cctaacgtag ggcagggttc cggcgccata  21585 cgggtcgcaa gaaacaggta caatcccctt tcccctaatt cattatgtca atatttttta  21645 ggttgtgggg cggcacatcc ggcaggatgt caatcgagat ttttgaatac aggtgatgga  21705 gggagtaacc gacacggtgc tggttttgac ggagcgcgac aggctcgccc tattcggacc  21765 cattctgttt cgaggagggg gacttacggc agcccggtgg gctgtttcag tagatgctgt  21825 tctaccgtgg accgaaaaaa ccatcccccc gttaaagggg tccaagctca tcattgctgg  21885 ggaaccttac ttggggttaa catcatctga tgttctctcc aagtgtgaag ggaaaacatt  21945 ttttgtgctc atcgctgttc cagcagccct cggacgctgg atttgcgcgc ccccgccacc  22005 gcttctagag tccgttgcag aaaatgtgcg ttgtaaaatt tcagcaaccc tatccaaaga  22065 gcatggagcg cccttttgca ctgccgtata cattttgggg tcaactacac aggttccccc  22125 tggctctagc ttaggttggt cttgtgtcgg gcaacctgtc cttcgggccc ctttaaaaga  22185 agctaccaag cccgacgaat cgttacaatc tgcagagtgt gtatttaagt gtccgctttt  22245 tgcggatgag ttttcgggga ataacccgtt tgcaattggg tttttgtgcg cactggacaa  22305 gggagttttg aagcacgaat tgtttgcagc aatttatgca gcaaaaaagg caatttcgaa  22365 tgcatcctta gacgggacat tttgtgcttc gtttggagat aaagagatga aattagaaag  22425 tgtaagcacc cacttagggc ctgccgttgt ggtaacgcgt ggggcaatta cagacttccc  22485 tccaaagaac gaatttatgg atcggcttac atgcaagcga acctttttgc gtaaagatgg  22545 ggactttctc ctgggatctg cgttcttaat gtgtaagcga caaaatagaa cgtcgccggt  22605 tttacgcagg tggaaaaggg cctggcttcg agataggcga aggaaacatt taatgcgcag  22665 ggcaagggtt tcggtaacag ctgagctggg ttgtcggatt ttatcgcatc caagcagaat  22725 agcatccttc gggctagaag ttacagaaag agatctgaat ttacagcgga ggctttcggc  22785 acatagaccg agggcccggg gggtggtata tgaagatttt gattagttac ctgaagagac  22845 acgccccaaa atcccaccca cgctg atg cat gcc  ttg ggc ata aaa tac  tct  22897                     Met His Ala  Leu Gly Ile Lys Tyr  Ser                                      1150                 1155              gcg atc ttc gca  gtg gtc agg cat act  tta tac gct cca act  tca     22942 Ala Ile Phe Ala  Val Val Arg His Thr  Leu Tyr Ala Pro Thr  Ser                       1160                 1165                 1170               gtt gtc atg gaa  cag cgg cga gac gaa  aaa gaa gcg gag gaa  cag     22987 Val Val Met Glu  Gln Arg Arg Asp Glu  Lys Glu Ala Glu Glu  Gln                       1175                 1180                 1185               aaa gta tct gca  ata gcc agt gcc ttt  ctc aat gtg gga caa  ttt     23032 Lys Val Ser Ala  Ile Ala Ser Ala Phe  Leu Asn Val Gly Gln  Phe                       1190                 1195                 1200               ttt tct cgt ggg  caa acg aac aag tct  cga ccg tgg gtc aca  tca     23077 Phe Ser Arg Gly  Gln Thr Asn Lys Ser  Arg Pro Trp Val Thr  Ser                       1205                 1210                 1215               tct gaa aca ata  aaa gtt tct ata tat  gca tgg act cgg tgc  aga     23122 Ser Glu Thr Ile  Lys Val Ser Ile Tyr  Ala Trp Thr Arg Cys  Arg                       1220                 1225                 1230               gac tct cta cta  ttt tca gat tac ttg  ttt cgc cgc ggc acc  atg     23167 Asp Ser Leu Leu  Phe Ser Asp Tyr Leu  Phe Arg Arg Gly Thr  Met                       1235                 1240                 1245               gat agt gcc cgg  cag cag ttg cag gtt  gga gtg cct aag aag  ggc     23212 Asp Ser Ala Arg  Gln Gln Leu Gln Val  Gly Val Pro Lys Lys  Gly                       1250                 1255                 1260               ggg gtg tac aga  cag cta tca cgt gat  gat gga acg gtg ttc  gag     23257 Gly Val Tyr Arg  Gln Leu Ser Arg Asp  Asp Gly Thr Val Phe  Glu                       1265                 1270                 1275               gtc agt ttg gat  cct gca gtg tgc ttc  gag ttc tca gtg acg  tta     23302 Val Ser Leu Asp  Pro Ala Val Cys Phe  Glu Phe Ser Val Thr  Leu                       1280                 1285                 1290               att tta ccg gga  cat gat ctt ttc tgg  ccg gta gtg ccg ccg  ctg     23347 Ile Leu Pro Gly  His Asp Leu Phe Trp  Pro Val Val Pro Pro  Leu                       1295                 1300                 1305               caa ttt ctc gag  ctg ata agt cag cgc  gcg gtt att ctg gca  gac     23392 Gln Phe Leu Glu  Leu Ile Ser Gln Arg  Ala Val Ile Leu Ala  Asp                       1310                 1315                 1320               cag ttt att tcg  tcc tct atc atg aag  cga gtg agc tta tct  cca     23437 Gln Phe Ile Ser  Ser Ser Ile Met Lys  Arg Val Ser Leu Ser  Pro                       1325                 1330                 1335               atc atg cta ttt  ccg aag aat aca ttc  atg ccc ggg tac tgg  tcc     23482 Ile Met Leu Phe  Pro Lys Asn Thr Phe  Met Pro Gly Tyr Trp  Ser                       1340                 1345                 1350               ccg gat ccc cag  cct ggt cgg tac cga  cca aaa ttt cag cct  act     23527 Pro Asp Pro Gln  Pro Gly Arg Tyr Arg  Pro Lys Phe Gln Pro  Thr                       1355                 1360                 1365               cga tca gaa caa  cat ttc atg acc gtc  ggc aag ctg gtg cca  ccg     23572 Arg Ser Glu Gln  His Phe Met Thr Val  Gly Lys Leu Val Pro  Pro                       1370                 1375                 1380               ttt caa att gac  ttg cat ggg aaa aag  aat aat ttc atg gct  gga     23617 Phe Gln Ile Asp  Leu His Gly Lys Lys  Asn Asn Phe Met Ala  Gly                       1385                 1390                 1395               att gcc gtg ggt  ttt cac ggc ccg ccg  acc ttg acg ggc act  att     23662 Ile Ala Val Gly  Phe His Gly Pro Pro  Thr Leu Thr Gly Thr  Ile                       1400                 1405                 1410               cgc gcc ctg act  gaa caa gca att cac  aac gct gtg gcc gag  gtg     23707 Arg Ala Leu Thr  Glu Gln Ala Ile His  Asn Ala Val Ala Glu  Val                       1415                 1420                 1425               gtt cga acg ctt  gaa ccg atg act gtg  gtt ccg atc act ctg  aaa     23752 Val Arg Thr Leu  Glu Pro Met Thr Val  Val Pro Ile Thr Leu  Lys                       1430                 1435                 1440               aat gga act gga  gct ctg atg gga ttg  act tca gac aac aag  ggt     23797 Asn Gly Thr Gly  Ala Leu Met Gly Leu  Thr Ser Asp Asn Lys  Gly                       1445                 1450                 1455               ctt cgg ata ttg  ata aaa ccg gca cta  gga gaa ttg gcc agg  tct     23842 Leu Arg Ile Leu  Ile Lys Pro Ala Leu  Gly Glu Leu Ala Arg  Ser                       1460                 1465                 1470               cca cga aga cgg  cgt tcc gaa tcg cgc  gga aga gag tct gtt  tta     23887 Pro Arg Arg Arg  Arg Ser Glu Ser Arg  Gly Arg Glu Ser Val  Leu                       1475                 1480                 1485               tta taa attgaataaa cgcagacttt ttattactgt caataattta tttattggtc     23943 Leu                                                                       tttattacac ggctccttcg ggtaacatta tacacgactc gattgcctct ctggcaacca  24003 ctaccgcgtt ctgcatagta gggatacaca gttcttgtta ttgcggcaaa acgaccaaaa  24063 tgcctggcaa aaaactgcaa aacctgtacg gggaatctat tacttgcgcc ccggccgttg  24123 taaaatctgg cgttggtcca ctttctgaca acatggcggc ggcgtaaaaa ctcacgtttc  24183 caagaatcga ataggacctg tcccatctca agtttgcgta ggttgctgcg gagagcgcat  24243 cggggagaga cagggattcc gcacgcgtga aggttttagg ccataatctc agatatggga  24303 cgaagtattg ctctccgaga aactcttctg ggaaggcgat tggggcgtca aatattccaa  24363 tgcgtactct tcgcgttaga catgttccgg aaaaatatat cggaatgtca agagatattg  24423 gggaagatgc tcccttgcag cgcttttcgt ataaaaagcg cgggcccaat atttccacta  24483 atattgcctg tagtgctggt gcaaataaag ggtcgcctgt gtgatcaaaa gccgattgaa  24543 tcccctgtat ctcatcactg gtcttgccaa agagttccgg tagatgctca ggcagtatgc  24603 tgatgtgatg ataagatctg ttagaaaata tccaacaagg gccctgagca ctaggagtag  24663 gcctttgctg tcgtatagag tttccgtgtc gttccttgtc taccatactg tcgagaagct  24723 ggttcgttat ttcagcattt tcttgtggtt catcatcttt tttacgccgg agtgtttggc  24783 gtatccagcc actccgaccg gagtgggagt tcttaactga aggtaatgat ggcgcgggtt  24843 tcagcggagg ggaaatcggc ccagatattt ccagtcggtg tagctgaggt tccgatcgag  24903 agaagcgctt gcggcgtcca aacggcatga atttgctcag ccagcccatg gtgtctcgcc  24963 tagagagttc tgagtttcag agcaggctct cgcctagaga gttctgagat tcagagcaga  25023 ccctcgaacg cgttcgaggg cttttaggtg cttgttgcat ggggcgtgta ctgggcgtgg  25083 atataagaca tgtgcctcga cgcccgccac gcacgatttc cgcagacatg agttccatgg  25143 cctgctctcg ttttcagaaa ccaaaagcga agaatgatag ttcgcccgaa aaatgtgaac  25203 gcactgccaa gcaaattgtc gcagcaagtg attattgcaa tgattcggaa aaattagagg  25263 aagagatggt cttcgtcgga gctggaaaga tttcatattc ttcgtggatg tacgaggcaa  25323 ttttttcctg tgattgtatt cccgacggat cagcgtttgc agttgctaag catgcagtga  25383 tcctaaatcg cgtaaacgga ggcttgccat cttgtcttgg gagtgcacat gggttgacca  25443 tttaccctgc caggacaagc cagcgtggaa ggttcagccg tttagcatac agaaatgata  25503 tgtccccagc tccacaatca gaaaatactg tcgatgtgga tggagagctt ggagcgtgcg  25563 ccatcggcgt gttggagatg ggcgaagaag tgtgggcaca tgaagaaatg ttttttttac  25623 cggtggttcc taagttagat cgcatgtggc cgtgtgttcc caatcctgct ctgttttggg  25683 gcctggcgcg cgcgcttaac aatcttcccc ccaagatact cgtttcagga aaacttgcca  25743 aagggttcat ttgccctcta tactatctta gcgtaccaac ttttggagaa gagagcaaag  25803 aaactttcct tcgcacgaga cagaagcagc gaagtctata tgaccctttg tttgaccctg  25863 cttcattact tgttcagcca caattttttg gatgtgatgg tattaaggat tctaataact  25923 ttcaagctgg gcttggcata gttttccagc ctcaggatgg atggacggca aaaacaaagc  25983 tgctcgagtt ggacagagtt cggtcggaac ttgtcacact atttgcacgc caggagcagt  26043 caaggcctat taccgtgata attactaggg gggcagtcag acgccggctt gtgttttcta  26103 ctgttggagg gtttaggttt tcatcctcgt tcgaaccctc tgggacaccc cttaaaaccc  26163 actcggtgac tttacatatt taatggaaca cacatacgaa ataaaagttt gtcagaaatg  26223 tatttgcgtt tattgatgtt tatgtttcgg gtaattatcc aaacagatca ggccgcgtcg  26283 caattcccat gttcttccat gcttcggaca ctgctgtgca agcagccgtc ttaaaatcgc  26343 ctatctcatc gggggtccaa tgagagtgca ataaaatgcc gagtctaggt ggatcgtacg  26403 cgtggcaaat tgctgcgcac agaaacttgc cgcacatggg agtttcttct aaaagagcgc  26463 gccagaaggt aaagcctcgc ccagtaagcc agctagtagc tcttgatgcc catgtatcta  26523 ccgctagata tgagcgttcg tccgatgcca atacccccga aactgggaaa aacgcgcaaa  26583 gaatgtgagt tcctttcgga atcgaggctg tctgctgtgc gcgcggtgga tgtatttggc  26643 tgtcgccaat cgcaacacaa aacgggatac ggaaagccca ttcttcgcgg caagtaatta  26703 tcggcgaagc tatctgtgat cgatacgcgc gtaacccagt aaatatcgcc gcttgtagta  26763 gtccgacggg cgagtgatgc gggctgtctc cgtgctttaa atcaaggatt tctccgctta  26823 gagtaagtga tggaggactt tgaaagaaca aggccccgct gaatgcgcgt acagcagcaa  26883 caaccggaga aagttccaaa tccgagataa gtcctaacat tagaaatccg actccatctt  26943 tccacttgtc tgcagacaac agtttaaaag gcaaatactt ggctccaaaa gtcatcgggt  27003 tttttgaatg cacgctatca gctacggctt gccatctttg gaaatttttt aaggaagctc  27063 ttttcatggc aagaaggagc tgctcgacgc caatctctaa attagaatta gttgtgtagg  27123 gagttctagt catgataatt gcagtagaag gaattccagt gtcgttggaa agggattctt  27183 gcggatcctc aaataatggt gctgctacga atcggatttg agagttatcg ggctgttttg  27243 agtcatggtc ttgaaacaca tgaatagtca aatccttgtt gcaatccaga aaaaacgatg  27303 ctgtgccgtg cagtacagct aggccatatt ctaccttttc tgtccatagt tttccacgat  27363 gtgttcctaa ttccatgtga gacgagtcta caaagtttgc gaggcagtga gttactgcaa  27423 aacgacattc gcgattgctg gtagacagtg ctttggaagc ctgagactgc ttctttgacg  27483 gagccatagc cagttattcg tgctgccgca agtgcagttg aaaagggttt gtaaggaaca  27543 aggattaaag ttcgctctcc tcgtcagacg gtagccgtac acgtctaaat tgcgttgccg  27603 agctgaggca gaatttatat atagccctga tggcactcca tagagcaacc aatccaacaa  27663 gtgcccctac cacaataccg ataatgtatg ccttgtcaaa aactattatg cttctgtcaa  27723 gtcctaaaat tcggacagca gttccgtgtg gtcctagaag cgcgtagtta tagaactcgg  27783 ggttcataga cttaaaaaat gcaatagatg agttggcacc ggctaacagt tctgattgaa  27843 catatgcact gtctataaag aaagcatcct ggaacccccg tagaccgtat tgaactatca  27903 ggtggttgca atatatgcaa gttttcgctc gtccattcaa atgaccatcc tccatttttg  27963 ctgttcgcgc gatttgcggt agggagttgc aacttgaatt cacaaacgtt acaaacaaag  28023 gggcagctgt tgaggtatcc ggaagtctgt aggtcattcc agtgctcggc gggctacttg  28083 tcatcaccca actagaatgc gcggtaacag ggaggaaaaa tacgatgtgg tgttccaggt  28143 cccagtttaa acatttcttt acctctggaa aaaacgcagt tgccaggatc tgaggggtca  28203 acagagcttg tggcggtatc ggtgcactat atgatggcaa ttcaccttca gatgataaat  28263 atattttggt gtaaaaaaat cgaaatggag cagatgctgt cagaattatt ttccatggtg  28323 cacttctgga aatatcaaac cttgtcgcaa ttgcacaagg agaaaaccac tcttcaattt  28383 tcgatgcctg tcctttcttg aagctcccgt ctggttggga gtgaatctcg ttgatatcgg  28443 ctagccggtc gacagagcat aaaattgtca tgcgcctgag gaagttcctt ccatgttgta  28503 tagaaagttg aggagcccgc aaaataatgt acgtagaaac tgccaataac tttctgtacg  28563 cgggggtcat taccttttgt gtaatgattt ctctgtaact gttatctatt atttcaagag  28623 ccagttggct gggggtcact ccatcgatgc tcgccaggaa tgcattttgg ggatcttgtt  28683 ctgcagtgat aagcaccgca ttcatgatag taagaagcag gttatgattt tttatcatgg  28743 gttcccatgg cctgtcgtgc ataattttaa gaatactgga tgggatagaa gtaagagcac  28803 caccgacatt ctgaataaag cgaatattcg ttgtatagtt tactgaattc gcccccccaa  28863 aacacactgc gacttggtct gcccatgttt gcgtttctgc aataaactcg acgatctccg  28923 aatcagttac gtatgcatcg atcgtaaact ttagaaatcg aaaccacgcg gccataaacc  28983 gtgctgcgaa tcttacaaaa aaagactcgt ctatggtatt cgggcaacct gcgattgcga  29043 ccttctgcaa agttaaaaag tgtcccgcgg atatttgttc gtcgaagctg gccagctcta  29103 agaaatcttg agcagcggtc gttactgctg taaacaaatt tggccgataa tttggtccag  29163 ctgcgacgtt gatgcgcaca gttaacccct gatcttcagc cttcagcata tagggccatg  29223 acttcaggga cgtttcttta accttcgcgc tggttggtgt aattatcatc tctatttgag  29283 gagaatgctg ggtcgcgatt ataagggtcg ccacattcag tccgatgtta atcttaagat  29343 ctatgtcact agtaactgcg aggctaatat atcgtgtaaa cacaaatgga aaaacagttt  29403 gtaaaggatc actgtaaatt acacgaatcg gagcgatgat gctctcgccc ctatgaactg  29463 agttatttgg tggaaagttg acgagtttcg cagatgtaag tgcattcccc ttgtatccta  29523 ccaaaagtcg atgctcaagc tttggatcgt tgctgaattt tcgtggttgg atggtagcct  29583 cgctagcaga accccatctc gagtaagtat aggctacgta cagttgtcca agcaaatcta  29643 gagcgatcga taaattcctg ttagcgcttc caggaggccc aaagattaag tttcgatcga  29703 caaggcatag ttgtgtgatg tggccgtttt cgactgctgt cgctatcgca atgaattctt  29763 tcggaggggc caaattttca ctggtggggg gcacgaagac aatttttgcc tttgccttgt  29823 catccccttt agctctaagt aatatcgcca gaatgaggaa aaatatttct ctcgcgtgtc  29883 ccatcggcgc gggttgctgg tgaagccacg ctctctcgag taagaatgag tacaaagcaa  29943 ggaaatgagt aaaggacata ctcttattgt gaaggattat ggatgcggaa cattacgaac  30003 ccatatcagc attgtagcgc tctactgcgt caattaagct cgcgatcgcc gctcgtttgg  30063 ttgtaagaga acttgccgtc tcggcaaact ctaaacattt cagtgcacat gcctccgggg  30123 tgaggccatg tgctggtaag taaaaaatgc acaagttttt caataccgtc gcgagttttt  30183 gaaccacaaa tgaaagtaca gtttttaatc tttccccgtg aacattttgc tgatcaaaag  30243 ctaatattgc aaatagcgtc tggtcgattg aaggattgat ttctggaaca gtagcagttt  30303 ctaagcactc tctcttgaat tttaagagcg tggcacttgt taaggcttct tgctccttca  30363 acaatgctgg ataagatgta cgttggaggt aggtggtagt attcatgagg cagatgtacg  30423 agttacgtaa tgcacacaaa tacgtccagt cgaccgtttc ccctgggcgc cccctttgta  30483 ttatacgccg tgcctgttcg caaatatcca aatccattaa aacaatgttg gagtgtgttg  30543 gaatgtcagg aattcctgac aataaagtta ttaaatctcc aaattcgagc gctccgctta  30603 gatattgtgc aacagggaaa cacaaacatg ccgcgagtgg gtgcctatct acgaggaata  30663 agacatcatc tggtccggat gcagctgcgc tgtagcagcg ttcagatccc aaataattat  30723 aaatgacgtg gttcattatt cttagtgggg cagcaatagt catctgaact tccgctacgc  30783 gacgagacgc ctcccgacat tcaagttttc cggaagcctt tagggtttgt atttcattaa  30843 tttctttgat catatccgct ccgccaaacc attggcgcca tgcttgcata ggttccgcta  30903 agtatagacg gcaacctctc caatttggca tttgcatcaa cgtctttgca gtgactgttt  30963 taccgactcc gaatggcccg tcgacgtaaa gaagtacaac gcggcgaaag tcggagtcat  31023 cgttctcgaa ctcggagcaa aactgcacac caccggaagt tttcacggcg ccagctacgg  31083 ccatctctgc gggctagact aaatgccgga atccgttgcc ataatcgctt ttaccgagct  31143 ctcgtcagat cactggagga agtctttgaa gggggtggag acggaaggct ggcctatacg  31203 ataattcccc aatgcaaacc tgccgggggc aaaattgtag tcatgtttga agtgaaccta  31263 gggttgcgga aacctgattg catttgtttg ctggagacac aacatgaaat gaaatgtatt  31323 gtgattgaac tgaaaacatg tcgtttttcc aaatctttga tgactgaaag taaattaaga  31383 caaggatata ctggcactct ccaacttcgc gattctgcac gattgttgga gaatctggct  31443 gttcctggca ctgagaaagt aaagattctt tctctactgg tatttgttgc tcaacggggg  31503 atgaacatct tggcggtaaa aacggttggg gaaacggtta taaatgtatc gtctgagctt  31563 ttctttgtca ctctagctac cagaagccag tatttaaaga ctttctgcgc gaaattagag  31623 ccacgcgtgt cgcatgcgcg cagtaagtat caacaagaaa gtgcaaagaa tgcggatcta  31683 gcgtctcccg ccccttcagc cttacaaacc gtagctgcat tgttttcgtc gcgcgtggga  31743 aaagagtccg caaccaaacc cgcgagctat tccacttcta cggaggagtc caaaaacttg  31803 tccgaaccat gtttcgaccc agattcgaac ctatgaattt accaaaggac agcaacaagc  31863 cgtctacctt gatggtttta gccgatcgtt taaactttat ctcctgcgcg gaaggctcga  31923 gtaaatatgc atctaagctt ttcgaaggaa ctctaataga cgcagaaatc atgaccaatc  31983 gtgcaaggat tgaagatctg gaaaggcgga acagggctgc caaggcagct ttagagcaac  32043 tggaaaatat gagcgctact gttcccgtac atgtctcatc ggcattgcaa actattgaat  32103 atcccctaga gactgtaatt gatgtcttgg atgatttagc ccagcgggcc gtgcaggaga  32163 aggacattgt tgggtcttat aaaacactag acatccgtgc gcctggcgag gatgtccctg  32223 cgaacgtaat ttggatagtt aagaatggag aaccattaac ttttaataca gattttcaag  32283 tagattttct gacaacttcg tttgctattg ctggcaatgg ccggctggga ttcgggtctt  32343 ggtttcgcgc gctacagact cagctcctag ataacaataa agctatcgct agggttttga  32403 atgtaatggg agacacgcgc atctcggggc ggtttatgaa aacggctatt cgagcgctgc  32463 gatcggcgat ggaaatttat gctgggactc gacaatatag cggattcgag gctactgttt  32523 tatgcttact gcactactcg cggtctagac aatctgcttc aaacattaga catggtcttg  32583 atgtttctat atttgaagat gcattaagac atgttccaac gtatttaaat tatatgctgg  32643 aagatattcg ggccgagtgg ggctcagtta cattttcatt tgaccggtca aaactccccg  32703 taaacttttt ttccccaatt gacggcagaa aatattcgaa tggagtgttc gatcctcaca  32763 tagtgtatca attgctaaag cgtactggga ccctttccac cacagtacga gatataacga  32823 aagaaactct gcttccaata gatccagatt ttgttcgttt tgatgacccc attgctgctc  32883 tctccatttc atttttccct tcgagacgaa cgccgttaat tcttcatgaa gatgatccgc  32943 ttgtgcgaac ggttatagac tcgatatctc tgctcctagt cttgcaaaag ctaatgttta  33003 atagtaatgt ctatacgagc acacatctca atcgatttca gccatctgcg ttctttgaat  33063 tgccacttgg aacccaatcg gaacaggagg cagctaaatg gcccgtcgcc cctgggtcaa  33123 gacctcaagc caccgcaagc acatttgatg acaatggaca agatatggcc agtcgcgaca  33183 acaacttgtt ctttttattc gagaaatatg ttgtacctat gtatagatat gacaacagat  33243 gtgaagttac tggtttcttt cctggtttag ctgcactctg cataacgggg cgcgttaaag  33303 gaataccgac agcggtacgg ttgggggagt actattcctc cctctgtaat ctaattgagc  33363 ttgatctcag aaaaacgtca catgtgggta gcggggcagc tgctgttttg gcggtacacg  33423 attcattgac aggtgacgtt gaagagggag tgtccaggtt acttgaggta tttgatgcaa  33483 aaaagcattt gaaaggaata ttgaggacat tcaacgtgga gtctgattct gacctgatct  33543 attttatgtg tcttggatgc ttgccacatc acgtgacata tggataatgc ttagtatttt  33603 cgcgatcgcg tctctgttta gacaataaaa gggttatatc tttctgatca gtccgtctgt  33663 tttgtcagtg tgttgatact ttgcgggtcg agtcggtctc gctatgtcgg agaacgtcga  33723 catcaaatat atcttcgttg ccggctattt ggtagtatat gaccaccaag aaagtgccgg  33783 gcgagaatat gagcttactc gcgagcaaag caaatcggct ttaccagttt tgcctggaac  33843 aattccgata aacattgacc acgagtcatc ctgcgtggta ggtacagtgc tgacgatctt  33903 agacctgccg agaggactgt tttgtttggg tgtcgtgtca actgctcttg cacctatttt  33963 tttaagctac gtgcaagatg atgctctttt tgcgaatgcc gaagagggga tggttttgac  34023 ggagacagag aaatttctct atcttctcag caacattcta ccttctctat cgctctcttc  34083 aaggcggctt gaaaagaacg aagtgcccgg aaaagatttt tttgcacacg tggcgctctg  34143 tgagttgggt agacgtgaag gcacggtagc aatttacggg gccaccgctt cggaagctat  34203 tggagcattt gatgatttga gcgcaccaat taaagaacag ctgtatgaaa tagctacacg  34263 tgagaaatgt gcagaagtcc ctagagagtt gtcgcgacca gaaattacgc gagtcctgat  34323 gaagaaattt attcacgggg cgtttttgat ggataggggt acctgtttga aaacgcgacg  34383 cgagatggca gctgtttaca acccgaaata tttacaagcg aatgaggtaa taaccatagg  34443 gattaaggaa cactccgaag agactcctga gaatgctatt aaagaccgtt cagtttctac  34503 gcaaacggcc ccaagcttcg atataagcga gagtcagcaa ccgtccgggc aaacacacgt  34563 tccagccatg gagtcggcga catgctccgg ccagttcttg caaacaaaaa acggagcttc  34623 accgtccgca tctcgtgaag atatggtcta tgtcccattc gagaagtatg cgagtcttct  34683 tgcggcatca gcccgcagag ataacgaccg gagacccgtg tcgccatcac gagagttttc  34743 ccgtaggtcg cgggactcta ctcacgaatg ttcaccagga cgtgatattt ggccgcgtgg  34803 attcgaacga caccctcgcc tagaatcttt tatgggtccg gggatgaacc acacttacag  34863 accggctctt tacgaagacc caaatttctg cgggcgtttc ccatatattc cttatcaaag  34923 ccccgcatct acttaccccg tgcatccaaa ttattatagt tcgaacttcg gccagtttcc  34983 tggggctgga acgtatccta tacagtatcc atcattacat gagcagactg ttgtttcacg  35043 cttagatgcc ttgatttcgg ctcttgagaa aaataataaa cgggatagcg aatactcgga  35103 gaataaccct cggaagagat ccgctaggac catttctgag aatgacccgt acttccctgg  35163 ggaaatggtt ccagcaaaaa agataactac ggagcagcag ctatgtgaaa aaaaggaacc  35223 cgttggtagc ggaattaatg atattttaca aggaattctg acccttcaaa aagaggttgc  35283 cggtcttaaa tcagcttcca atgccgattc gtcttcagag agaaaagagg aactagaaaa  35343 tagtaatcaa gagtctgctc gggagacagt cgatgcttct atgccaaaac gactgaaaga  35403 tgctcagaca aaattgaaaa gaaagaaaga agcagccgca tttgcgcaaa tgatggcaga  35463 ctgagtcatg tgaatagtaa tgtcatttta ttaaataaac tgcatttaaa cttattcgtc  35523 ttcgctttct tctgcccacg aagggtattc gagcaggggg tagtcatctt ccttggttcg  35583 gggttttctg cgaaataggt ttctcatatt tttagcaaaa gcagaaaacc gcgctttctg  35643 tcttcgagct ttctgttcat ccttgtgtag aagctcaagg cctttcagaa ctctggtggc  35703 aagtatcctg tcgtcctcat cactatcctc ctcaacctcc tcctcatcct tgtagtaacg  35763 tctagagggt cgtggaggag ttccggccgg gggaactgcg cctgggaaca gaacctgaac  35823 tgggtttgat ttcaggttca ttacatattt gaacgccagc agtcctaaaa taatgctcac  35883 caccaccgct atcccaattc cgagtgcggc gaacgggtta gaaagaaatg aggcgatgcc  35943 agatactgta gaaattactg ctgccgcggc tgtcatcact acagttccca atgcctttcc  36003 tacttctcca agagtgttgc caaaaaaatc tgctattgct ctaaagattg catctcccct  36063 atctccacgt attacagtgt caatgtctct gaaccttttg ttataaatat tttgatatct  36123 gaccacatca tcatagttta aagtcccagt atctctgagt tcctcgcgag tgtatacttc  36183 gacgggcaca aagtccaaat cttctagtat agtggcgttc aagttgatga atatgctcac  36243 ttcttcgatc tcggaagcat ttacttgtct aacaaatgta tagtcttcgt aaagaaggta  36303 gttttctccc aacagaaagt accgtctgtg attgatcatg catggttcta tcaaattccg  36363 cccttgtaaa atttcattat gttctccgag ttggcctaat atgtcaagat tgtggttttc  36423 tgttgagttc gcagaaaact gtgactcagg ggaggacgaa tacctgaaaa taagtactgg  36483 tctggtatag cacatggttg ggtcccctgg catgcgcatg gaatcctgca tcctaatatt  36543 ttcgattgga atttctatac attttgatac cgctacgatg tctcccaata gcctggcgct  36603 tacaggttgt ccgaacaaag atgtcatcag tgagttcggg tttagtttct tcatctcatg  36663 ccatacaatc agttggcggt tctgaagctc acaccacgct tccaacaaat ttccgataag  36723 ctcgttaaca tgggcttgga ttttgtcgta tgcaaattgt aacatggcaa atgttgcaga  36783 actgtgggta gtaattctat caccctgagg tccagactgc agcgacgccg tgcggcgacc  36843 tgcggcttgg cgacgctctc tccctctcgg gagatgattt tgtctttgtg cctcttctag  36903 gtacatttca gccaagccat ggctcatgag tttctgaaat gcgatcagaa atccgccact  36963 accgagatag tattcgatgt ccccagaacg gacatgagtc gaactatact ttctggcaaa  37023 gatgccatct atggcctccg aggctatggt aggaacgcat tcagccaaat gaagcctgct  37083 gatgttaaaa ggttgttttc cggacgagaa cgtcatagtc gtatctttaa gtgaaaagtg  37143 gtaactgttt ttgtacgaaa cacgaactgc ttccgggact tcaatccatt tactgagagt  37203 acatacagat tccttttctt ccattgcatc ccagcctata gtgaattgtt catctgttag  37263 aaagtttctt tttttagggg gtcttatttg tccggtttcc aaatccctga cttgataatt  37323 tgcgatttcg agaaatctat agtctctgta gacactgtga cgccttggtc cggtcgtgtt  37383 tttggtataa aaaggagaaa tttctactgt atcacctgtc gccattccaa agtaatcata  37443 cgggtataca gatctagcct gtagatattc cacaacacag tcgaccgatg tagacgttcg  37503 gtatcccagg gtttgatgtc gcttagtaaa gttagttgta tgaaacgcct tggagacagt  37563 tgatcttaac agtgatggaa ccaggggcaa ttttttttct gcttcatcat tgtcgtaagc  37623 ttcaaaaaac atgaaatttt tatgatacgt tgctttggat gagcattctc ccgatcgatc  37683 aatcctgaca atttcatgat agtctattgg aaccctggtc acgtactcat ttgttatttg  37743 gggtcttgag aataatgccc acgtagtaac tgtggttata tgtttatagt atagagtcac  37803 attaaagacg tacggggcaa tgttttgctt gaagactacg gcaattcctt cagtcatgtt  37863 agtcgaatcg gcatgtcggt gacaatgtcg tggctgggca agcctcgcaa cactggcgcc  37923 agttggagat gagcacgcaa aaattctgtt tctaatctgg ggttctttcg gtgcccccga  37983 cccaatggct tcggagaaag agatttttcc aacatccatg ctggctgtat cacgagggtc  38043 tattattcca gcaattccgt gtgaatcttg agatagggta gatgggatca ggattgccac  38103 gcacacgcag atcagcattt tcaagctagc catgtcaatg ttcacggcga tgtaggattg  38163 catacaggac cgcgtataaa tctttctcta ctatgatagt agacgctgga cccagtttcc  38223 cgcggtcatc gacgccccac actgcaacta atggatgttc ctcttcaaat gttaaatata  38283 gcccgttttc caaaatcggg ccgtcgaggt cgaattcgac acaatctgct cgcagtaact  38343 tagtgttacc gctgtggaaa attttctcga aagccgttgt agcaaagagt gcctctctga  38403 tatatccgca cgcggctctc tgaatagcag ttgaattaga aatgcccgcg aagttataga  38463 atcggcgaaa ctcacttgtc atccaatcac atgatctgtg acgttttgta aagttggcca  38523 actcttcttt caaatgagga agaagtccta cgttctctac agaaaagtat atagacgtgt  38583 ttgggggttg gggaaatctc tccttgtgat gtttgaaaag ggggccgctt aaaatttcaa  38643 aaaaccgatt ggtgaggcgc ggtaacatgg tttggtcgat ttggtatctg gtgatggaag  38703 agcgtatgta ttgatgccaa tcataagcac gcccatcatt ttgatcgatg cgtagaattt  38763 tcgaatgtag agactcttca gcgcaaacaa tatccagaaa tgcgcgtctt gctagaaacg  38823 cattctttag cgttacgtac attaccggaa gagtttctcc gtaaatattc acacgcatta  38883 atttttctag ctcggctcgc tgaattttaa tgcatcgatc caagtttgaa aatgatcgtg  38943 cagaaagctt ttcgacgtag ttcttttgtc tgacatcaag atctcgctct gcctttttta  39003 gaagaacttc tatcgaatcc tcgtcccttt caatagttcc tctgaggtta gcagtcaggt  39063 cgcttgtact agttccttct gtgacaggtt gtgtctcctt gtctgagcca cgcagttttt  39123 ccactaattc ctccagtgca tctgtatcta ctttctctgg gtttcttaat ctgcgaaaca  39183 atgggaggga tagatggtgg tcatagcagg ctttgatcaa ggcagctatt tcggtatccg  39243 gtgaagtggc cagtactcct gtgatcagcg tatccactaa agaatgttgt ggggatatta  39303 actcagctcg cgcgaccctg tgaaaatgtg catgcacctt ttcaaacagt gttctttcta  39363 gacggatgca ttcctgttca aagtcttcag ctcttttgaa cagttcgctc agatctttgt  39423 ctaactgctt taacacagat tgtgaagtag tagtttgtga ctctactccg ctgggggtcc  39483 ttttacccga cgctagccag tattgtaatt cacttagttg atagacgggc cccggtggtt  39543 ccgtaaacac atcatatttg tttaagattt tggaagcttc tgactctcca ggggaacttg  39603 ataaggtgcg tcctgcttcg acgtggtttt cagcttcgat gcgagtctcg tttgaaagca  39663 cattttttaa tttttcggga tccacatccc ttaaaaatgg aagatggcgt ttaaggtcat  39723 ccgtagatac attgaccgga aactgtcttg tcaagtgatc acaaattttg tatccaaggc  39783 gtcgataaat tgtttctccc tgattagcgg ttacactaag ctcttctaaa cacacataac  39843 acggctgacc atgatcgtaa gtatccggtg ctgctacgag acttcccccc gagctgagaa  39903 ccaaaaactc atctattgta tttagagctt caattgcgtt tttacaagat acaacgcagt  39963 gacagtagtt tagttgcttc ataaagtttt ctacatcgtt gatcagaacg agttcctttg  40023 aaataattga actcattcca tataattgta gctggacctc cgcatgcaaa ggacagatga  40083 atcctttccc gtacccaagc gtagcttcaa aaaaacattt tctatccttt aggacagaat  40143 acttgtctat ctcattacac aaagctgcta tttcggagca cgctcttcga agccagagcc  40203 acacgcaata tgtctgaagg gttaacctat aatttacttg catagagagc tcattagaaa  40263 ggagcgaatg aatgtacaat gccattagcg cgttgtgttt gattctcgac cgcagtcgct  40323 tctgcaacaa tatctctgga tcgcagcgct tcaaaatatc tatttgaaat aacagcgtct  40383 gtagctgacc tgccagagcc ataaggcgta actgtgtttc ttcagccata ttccccagta  40443 agaatgaaac aaaatacgta caaggacttt cttatattta tttgtttatt ctgagcatgc  40503 gcagttactg ttaacagaac agaatatcag ccgatagcga aggttttttt gcacttggcg  40563 cgccaagttc aatatcttcg ctgatgtcgc cagtgtttgt atcttcatcc tcgtctgccc  40623 agaccaacat ctctcctgca ttttccgggc atgtatcttt cccctctagc tcactgagca  40683 tactttgtgc ggtagccacg gaccacccat tctgacttcc agaaatgtcc tcatggagct  40743 ctactaatat atctaccaga tacttgtcgt cagtaattgc catccatgtt tcggcagtaa  40803 tgtctttcat tccttctccc agaactctca gaacggctgt gtagatggaa gtgggactac  40863 ctcctgcatc aattaggctt ctcacttgag aggcaatggt gttttcttga aaccctgatg  40923 ccacttgccc agatgacaga atagttccta cccttttaca agcaagtgca aactttctga  40983 cgggatcatt cgtcagcgcg gaattaaccg attgggaccc tgaattgcct ataaggctac  41043 cccaattgcc ggactgaaag acggtggtcg agttttgttg tcctacatat ttgctgatca  41103 taatccctaa cattactata ggtttagaac tcaataccac gcggcacgag tttgacgtac  41163 tggatccgct tatgcagtag tcagagactt cttcgtgcct ttcctctgac tggtgtgcac  41223 ctgtgtctcc gagttgtgcg cgcgcgaaac tctctttgcc ctcacttgtt ggctttatgg  41283 aggtcgtaat gatcatcgat ggagtactta tataatattg tgctctgtcg gccatctctc  41343 cgtggtcgca gaactccaac accttgttgg cgagcataaa atttgctagt tcaagcaggg  41403 tagttgggga gcacttaata aagtttccct ccccatagac ctttgaagtt tttcttataa  41463 attccaaata ggtaatgtgc tctggagaaa ggcgtcgttt gggcatattc ccagtcaaga  41523 tcaaattcca gaatcgaaat gcgttcatgt ctttgccgaa aagatttgca tggtcacgct  41583 tgagtataaa tcccaatggt ccacccaaaa tattgattgt tttggtgccc ggtttcaaaa  41643 aggcatcagc cattccttgc aatctgcctc tgttgctacc cgactcgctg gatccgttga  41703 acacgacgcg gttcttcacc ttaaactctt tcagtttcag gacttgtccc ctagaaagag  41763 aatactcata ttgtgcgatt gcctgttctg tgtttgggcg cattgtatcc ggagcgttaa  41823 cacacggaac tgatattgaa aatggctcat attttccaga aaggaatcct ttgtccagca  41883 tcccgttaaa ggctgatctt agggccggca tggcagaaga acggaacatt tttgactctg  41943 gttgccgcgt atcaaatgtt ccatggattt gactgagaat caaatcttgg aaaagtattg  42003 agcgagtctg ggcgcacaat aagctaagta ctggacagta cgcagaagaa tacgggttca  42063 gggtgattcc aaatgtatgt acgattgagg ataagtgctc tttgtaatga tatgctctca  42123 ctcctgagag ggcagaaatg aacttagagc attctgatcc aacgaagttc tgaatttttt  42183 caaatacagt ttttaattct gctaggcttg aaatattatg cgtgtcttcg cccgtgtctt  42243 tgtttatcaa cccttctgat actagatatt gaaagactcg ccagcctatg cccctgtatg  42303 tgtcattcat aactgctttg gctggttcac cgtcacctgc cctctttaac tgggaatacg  42363 gggcaaaatt ccccaaaatg tcgcagtccg tgtagtctcc atcaactgct cctatcactg  42423 cgatagggct tcttctcatg gttcgtggaa taggaaaacg ggttctcagt cgctgcatag  42483 tgtaacggat gcaatgattc ctagactcgt cattgcacca ccggcattct ggtacggctc  42543 cagtacaagg cgcttttcca ctgttagaaa tcgaaatcag attcatgttt ggccgagtgc  42603 tgagttgctt ggaacatttt tccaaataaa atatgatacg ggacaggagt tcggggctaa  42663 aaccacaggc atatgcgagg tgctcaggat catattcgaa tgtgttctga ggtgtgagtg  42723 gagatggaca tgtgccttcc caaactctct tccctgagta gtccacctgg gggcatccca  42783 ataaatgcaa tccacatgtc agataaaaac gggttagtcc gctcgaggcg cgctcgccgg  42843 gctccggcgc acccccatcg actacctcac ttgcataaag gggggagtta tttgaaaaaa  42903 ttgctgctcc tacaatgccg gccaattctg ctgagaaccg atcgagagct tgaacgcgct  42963 ccatttgagt tttgcaatcg cagaacaacg gccactcttc atacggcaga ccgcagccat  43023 tatcaaaggg atatggagcg caggagacag agagtctagt tgccagcgca agttcagcag  43083 ccaagctaca ggcggcgcgc cgttctactt ctacattttg tttagggggt cctgtttgtt  43143 gtatgttctg agccagctcg gtaaaggtct tgtccgaggt taatacacag ccttcgaagc  43203 ttccatcgaa cgcgagcatt gccgctccgc gcgccacggc ttctaggtct ctggttctca  43263 gactttgtcc ctgtgttccg agtgttgtcc catgaatgag tctgttcaaa gctttgttga  43323 ataatggggt agggtaaatg cagccttcac cgatgttacg gctgtttgaa tcaaatgggt  43383 tacggcaaag ccgcagcgcg atgtctggca tgtacatttg cacagggtag attggtatgc  43443 gcgtaacaag cttagaattg atgtaaagcg tctcgactga tccatagtga atgtatgtgt  43503 tgcatacata caccgcctca caaaacggtt cggcaactac gagatagagc atcgttttgt  43563 cagggtccat attcagggat ttgcagatct cttctcctgt agtttcgatg gcatttggaa  43623 ccggtccatt tgggggtagt gaactgaacc cgaacctttc ccttgcgtcc tcgcatattt  43683 ttgtgaggtt tggagtgaat gatgtaggcg ggatgcactc tcctccatga aagatatata  43743 ccgttgttga aaaatggcag ggagataaaa tagccgtaca attacctcca acgactcctg  43803 atgacttagt tccgatgact gctgcgacgt ttggcttgaa atcagactct acagtaagcc  43863 cccgtatgag aggagcgatg gcgcaggagg gctggtcttg acttttggcg cacagtatct  43923 tccattcctt tccatcaatc tcttcgcggg gtcttgcata tacgtagcca attggcccaa  43983 tacaccatgg tccgccatcc gactttccgc ccttagttgt gccagatgaa ctcttttcca  44043 tttgcgcgag ttatctcaga cagtatgatc tcagatcaga gttccgcgta gtagagtagt  44103 ctccgtcact gagcgcggac ttaagtaccg cggaaaggac acacctatgt atactttaga  44163 gagttcataa atatcgatga ttgcagtagc tgggcaccga gtttgtcact ctgcactatg  44223 gacacttttt cctccgccgg tactggagct gctcgtgtag ccccgataat tggcctcggg  44283 ccgcactctg gcgcgtctta ttatacttct gtgagagaat tcccgtatgt atgtccaacg  44343 tgcattaatg ggggcggaag gataggaacg cagattggaa gggctacaag caaaccaacc  44403 ttttaccaca acgagcgaca attagacatc ttgacaagca cacatggagc gtggcccgtc  44463 aggatgaaat attggaatgc tggtcctgga atgactcacc acaacaaggg agatgtaaag  44523 ttttatgaat ttcacgttta tgaccttttt gaaaacactg agatcgctca gtcatgtgta  44583 cgatggatgc attccagatt tcttgataca ctgaaaccga ccggtactgt aataacctta  44643 gttggaaatt ctgcatgcgg aaagcgcgtc gccgttcatg tgtatggatc tttgccatac  44703 ttttttgtta aaaagcgaga aattgaccaa gccgcgaaag tcacgaactc ggaagagttg  44763 gcgcatgctc tagcattagc gacgcgtaag acatctttaa aaaattcacc ttttctagct  44823 gtttcagcgg aaagtttttg cattgatgtt gttcggcgca gagacattta ttattttgaa  44883 tctgaggagg aggacttcta ccgtgtaaaa gtgtgtaatg gcaaagtgat gaaatttata  44943 tgtgataatt ttttcccgag cgtacctaaa tatgaaagca atgttgacgc tattaccagg  45003 ttcatcctcg acaataatct gacttctttt gggtggtatc gctttagagc tcaaggtggc  45063 gcactccaaa ttcgagaccc aggacaacat gctacatcag ctgatgtcga aattaactgc  45123 acagcaagta atttggaact cggaaacaat gtctcgtggc cagactacaa attactgtgc  45183 tttgatatag aatgtaaagc cggaggggca aatgagtttg catttccttc tgctgagaag  45243 gtagaagatc ttgttatcca gatttcggca gttacatatt ctttgttaac taaggaaaaa  45303 gagcaggaaa tcattttttc cctagggacc tgtgaacttt ccgaagattg tagtgatggg  45363 ataactgtat gcgaatgtgg ctctgagttc gagcttcttc tgtgctttat gacttttttc  45423 aagcaatatt cacccgaatt tgtgagcgga tacaatattc tagggttcga ttgggcctat  45483 ctttctaaca agctgagcaa ggtgtatggc atgcgtctcg atggatatgg gaaagcaaac  45543 tcgtggggga cattcaaact tcaagatccg tctgcgcgcg gacttggaag attcaaaaaa  45603 gtcaaaatca atggcgtcgt caacattgat atgtttacca ttagttatga aaagctaaag  45663 ttaccatctt ataagttaaa tgcagtagca gagtgcgttc ttggagaaca aaaaattgat  45723 ctggcttaca aagatatccc agtcatgttt gctgcaggcc ctaaagaaag ggggaaaata  45783 ggggagtatt gtttgcagga ttctagactg tcagggagtt tgtttttcaa attctcccct  45843 catttagaaa tgtccgctgt cgctaaattg gcctgtatac ccttgacaag agcaataggc  45903 gatggacagc agctgcgcgt ttatacatgc ttgctccaac gctctacggc gtccgggttt  45963 gtgttgccgg acaaaaagag tgcgttttcc ttcggttcta ctcttgcttc agacgcgtct  46023 gatgcacctg ctacaagaaa tgtaggctac cagggagcca aggtactaga tccagaaata  46083 ggattccatg taactcctgt ggttgtattc gattttgcca gtttgtatcc gagtattatt  46143 cagagtaaca atctatgtta cagtacgtta acgcacgacc ccgccgcact ggcaggtcta  46203 cagccagaaa aagatttctt ccagattgat gttcagggtc ggaaatttta ttttgtcagg  46263 gagcatattc gcaaaagttt gctgtcagac ttgctaggtg attggttatc tatgaggaaa  46323 gccgtgcgcg caaaaatcaa gacagccgag actgaagaag agcgaatctt attagacaaa  46383 cagcaagctg ccattaaagt tgtctgtaac tctgtatatg ggtttactgg ggtgatgcat  46443 ggaatgcttc cttgtttaga agttgcttca acagttacag ctataggaag ggacatgctt  46503 ttgcgtacaa aggcgcacat cgagaaggag tggagaagtg gaaatcagtt tgccgaaaaa  46563 tttttgcccg ggtccgaacg tattcagcta aaccaatact ctgtccgggt catttatgga  46623 gatactgatt ctgtatttgt gaagtttacc ggagttgaca ttgaaacttt aactcaagtt  46683 ggtggctgca tggccgatga gattacacaa gcgcttttta gagctccggt aaaacttgag  46743 tgcgaaaaga ttttcgtgcg cctgttactc attgctaaga aaaaatacat tggggtgatg  46803 catggaggta aaatgttgat gaagggcgta gatctagtca ggaaaacaaa ctgtaaattt  46863 gtcaatacca cagcctcccg attagtgaat ctacttttcg aagacgacga aatagcgatg  46923 gctgcagata cagcaagagc cggatttgag gattttgact gtcttccgag cggacttact  46983 aagcttggcc ggttgattgc agaggcaagg ctagctatta ccggcaacgg actaaacatt  47043 agggacttca taatgaccgc ggaattaagt cgtgcggtgg ataactatgc cagtttgaaa  47103 ttgcctcatc tcacggttta tcaaaagaaa gctgctcggc gcgaagaatt acctcaaatt  47163 aaagaaagaa tcgaatatgt catcatagaa ccgggacaat ctgtcccgaa tgatccagca  47223 actgagtctt ttcctgaact aaaagaaact tctttgattt ctagcctggc agaagatcca  47283 gaatgggtcg tatcaaatgg tctaaaattg aatgtagaat actactttga ttcgcttatt  47343 agaacattaa gtgtaacatt taacgcaatt ttcggtgatg caaaaacagc agaggatgtt  47403 ttaagaagct ttattccaga gaaaatacag ttttctgaga aagttgcaga ggctcttgca  47463 cgaaacaccg cgacatttgt atccatcaag aagtaatcgg agggctgtaa gttgcaaatg  47523 ttgaataaag tttagtgtat tcgtgaattt tttcgtctgt tactaggata tcgcagtatc  47583 gtcgatacac tgactccggg ttcagcttaa cgcgctgatg ctcagagtaa cccggtccat  47643 cttctttgga aacgatcaat aagaacgtac cagcgaatac gtccacgtgc caagtgtatc  47703 ctgggcaatt aaatattaat tgtttcagaa gctttgcccc tagatgaagt ggcgtgctct  47763 gtttaaatac aaaatacata aggtatcccg atggaaactt cttagatctt ggatcgttca  47823 ccaagagaac ggtaaaatct tgagagagtc cgccctttac tgtatagtat gcataaaaca  47883 aagtcgcctg ggccagtaga tttttgatag tgtcaagtag gaattcgttt tcatatgctc  47943 cagatccaac taccgattca cctgaaaata cccgcaggga cgccactaac tctttgaatt  48003 cgaccacaga gctcaggtga tggagaaagg ccaattccaa cgcaggcaag tctaacggtg  48063 gggcatttga ccatccatat gagcaaagtg agcagcatcc accaggtcca acggtgtagc  48123 ccatctcgga aagagagata cagtcgccag gggcaaaact tgcattcaca ttgtaaggaa  48183 gggagatatt ttcttggcgt atgacaggaa tcgtcattgt tctaagaagg gagatagtat  48243 cttccggaga tgaagccgtt agcttgaaaa aagcatcgaa gaaagttctc cgtgtgtcta  48303 gattagccct tgacctagac gatcgcctct tcgatctgag gtatgagatg tagcgatggc  48363 gttcgatggc ccccgtaata ctttttcttc ggatgcgcag agcagcggcg cgagaacgga  48423 agtcagacat atcgagtcag tgcgtgttct aaagcaatgc agctgattcc gtgcctcttt  48483 aaataggtct cagcgccgcg aatgaaagaa tttaaagcgg tagggcgagg aggagaaagc  48543 tggtaagctt taaatgtgaa ggctagaatc cacagcccgc ggcaaacccg ttcttgaaat  48603 atgttagccg tggcgatttc ggctttataa agctgcagca gttctgggtc cttgggttgg  48663 ccttcccaca aaacttttgg atttacgcga agcgtattaa ctgcacacaa tggatcgcaa  48723 aaaaagtgtt tgtaaattgc gtttgccccg gccatcctca ataaggttaa cattcgcccc  48783 ccatcgttta acgcagtata gtcttcgaac gtttctaatg ctggttcaat cccatcgaac  48843 aagctgctat ttacgttact gcagccgact actttacttt tcagctttcg aagagcatgc  48903 cagtgttccc gagtaagcat gagattgcac aacacgcatt cccctccagt atctcctcgg  48963 ctagtcgggt gaggcaaaat agacgccaaa attgggccaa tggcaagtga tggtttggta  49023 ttgtcgcaaa tgtactgctt cgctgtcttg gggagcctag tatttttgac ctgttggatt  49083 agcgttgcgt gaaagttctc cactacggta tttcttgtcc gaattatagg ggcaacgaaa  49143 ttttctccat tatcatttgt atcatcatac tcaggctcgt cgctgcagct ggcatcgagt  49203 tcccgatttg gcataccggc tgttccagca attaataatg ccgtcaggtc ggtaaatccc  49263 catttacttg gggttgtcag ggtttcgctt ggtccccctt ccacatattt tgactccgtg  49323 ctaatatctt ctaaagcagc tttttgcgca caggttcgaa aagcgaagga ggcagtctct  49383 gtatttttcc tctttaacat acactgcgcg gagcaaaaac actcgagtgg tctggcatac  49443 tcacgccatt ttaacaaagc cacggccatt ccgctacttt tttggaagtt gaaatctgca  49503 gacgcgccag agaccggggc aatttttgcc ctaagtgctt cttgatatct agtagatagt  49563 aacgcatagc gtgctaccga ctgcactagg aacgaataat tatttagatc tattttagag  49623 tccagtcccc cttccaccgc tttgaaacag ccgttaaaaa agaagtgctt ctgaatatcc  49683 ccgagggaaa attcccgacg gcttatagcg ccaactaaag aggtgctgtg ggaatgcaag  49743 tagtgtttgt ccatgatgaa gatgaactca aaagcagtta tgaaggtaga tgtagcacac  49803 agggggatgc tttgagattt gaagcacaat aaagagtagt ccaccatcca ttcgggagtc  49863 acggggaatt ttttttgata tttggtaata atcttgcaga cggcgcatga tttatctagg  49923 gtgtataagt cagccatggt ttctacgaaa gaatcaatgt ccggaattct gttttgttct  49983 tcagcttgtt ctgtactttc ttcatgatat atttcaatta ggtgagctcc atacaatagt  50043 tcgtccgaca acttgtcatt aaacgtcaga atcgccgggt cgtagccagt atatagtttt  50103 gagagcactc cagctttcca tcccttggaa agttcagtgc gagtttctgc agttgatcgt  50163 ttggatgtca tggcatccac agttccgagg cgtcttagtg aattggtaga tgaagaggag  50223 ttagcggaaa ggccactctc agaactgttc agaaagtata ttggatctgc aggtagtccg  50283 gtatatgaaa tatggtttta tgacctatcg cctccggaaa ttgaagtagc tctgcctact  50343 accgatgcta aactaaacta ccttgcgcac acagccaatg tcgcggccga acttcggtac  50403 cgcaatttag atgggaaaag gatgtgcgca cacgcagaac ttattgctcg ccgtcgtgaa  50463 cggttcgcgc aaattctaag taagtttttg gatcttcacc aaattttagg ggcgctagaa  50523 ccttgttagt ctggtagtat tataaataag agggcgagtg tgtatggtat taagacggat  50583 ccagaagacc atgaagaaag caatgaaacc ccaaggcgcc agaaatcaag gagacaaata  50643 ctgtcaattg gtgcgcgtcg tgaatgctgg attaagcctt gccggaacga cagcgagtct  50703 ggtgtatacc agagataatg cacgtctcgc acccactgga gatattttta cgcttcttgc  50763 taaacttgac ggtcccccaa ttccagcgga atatatccta gaggcaatga atagcttcct  50823 taatattgga gagtcgtggc tgcgtattca aaatacgggc caggctgtca ttgttgccgg  50883 atgctctacc aagaccgcta attgcaaaga ccaaatatgg cctgcttcta gtcctaccat  50943 atctttagcg gctgcaaaat ctctgtgggt aagttcctca tcggtgaagg aaatgaaaag  51003 acttcaaaag ttaaaaaacg ccccgctcgc aacaatgatg tacattagtt tttacagggg  51063 gtcgagaact gatattacca tcagattcgc attttaccgc tcagactcgg aacccaattt  51123 gattaaaatt actcgcagag tgcaagaggc tatagactcc gcggagcaag aagattcata  51183 taaaacggcg gcccttctta aaaaaacgtt gatggaaact acaagcgaga tgcccacaag  51243 tcactcggtt gctgagtcga agccatcacg ttctattttc gagcgcgtca ccacatattt  51303 tcgaatacat ctaaaatgtt taaacccgag gtccttctct ttacagccac tgatgtgggt  51363 gcttatgggt gtggcatggc ctgctttttt gacactactg atcttctatt taatccgagt  51423 acaggcgacg agctgaaatc ataccacacc tggaggcaga tatgagcagg caatctaact  51483 ctcattccac gccccaacca caggacagag tcaatgcggc cgcagaaatg ttgaaagctt  51543 ttttattgac ccctccgaga gatcgagctc cggactacat gcaaacctta aacggaatta  51603 ccttggatca actcacggaa gtagctcgag ccttgaaaaa tgaaatcccg agagaagata  51663 cagttcggca aaatattctt agaaataata tcacactcgc gctaatggct ttaaaccgag  51723 ctccaatgct tcgagatcgg ttaaatataa ggcccgtatt tgctcgcctt tcaggccctc  51783 aaggattgtg gacgtttgga gtgagacaac gcaccaggcc aagaccgaga cgctaagcaa  51843 aacgcaataa atataccaat tta acc cag  tat tat ttt tat ttt  gtg tgt    51893                       Leu Thr Gln  Tyr Tyr Phe Tyr Phe  Val Cys                                        1490                 1495                  ttc atc cgc  tat gcg ggc ctg ctg  atc taa agc gta atc tct  cgc     51938 Phe Ile Arg  Tyr Ala Gly Leu Leu  Ile     Ser Val Ile Ser  Arg                   1500                 1505                     1510               gtg ttt tat tcc  ttt aag cac cat gcg  gca ggt gac tga cag cga      51983 Val Phe Tyr Ser  Phe Lys His His Ala  Ala Gly Asp     Gln Arg                        1515                 1520                     1525           tgc ata aat atc act  gcg cgc ggt ggg tgg  caa ggg act aag aat      52028 Cys Ile Asn Ile Thr  Ala Arg Gly Gly Trp  Gln Gly Thr Lys Asn                            1530                 1535                 1540           ttc tct ttc acc cgc  ttt aga ctc ttc cgc  atc gct tgc aag gtc      52073 Phe Ser Phe Thr Arg  Phe Arg Leu Phe Arg  Ile Ala Cys Lys Val                            1545                 1550                 1555           ttc tga tgg ggc ttt gtc  att tat tga ggt tcc gga  aga tat ctc att  52121 Phe     Trp Gly Phe Val  Ile Tyr     Gly Ser Gly  Arg Tyr Leu Ile                            1560                     1565                        tac  agc atc atc ctc ttc  cga act aga atc agg  tgg agt ctg aat     52166 Tyr  Ser Ile Ile Leu Phe  Arg Thr Arg Ile Arg  Trp Ser Leu Asn           1570                 1575                 1580                           gga  ttt cag ata ggg tgt  gat cgt tcc atc ccg  caa tgc gtt gtc     52211 Gly  Phe Gln Ile Gly Cys  Asp Arg Ser Ile Pro  Gln Cys Val Val           1585                 1590                 1595                           caa  ttt tct caa ata agt  atc taa agc ttg agt atc  cct tac taa     52256 Gln  Phe Ser Gln Ile Ser  Ile     Ser Leu Ser Ile  Pro Tyr               1600                 1605                     1610                       cct ttc tcc  ctc ttc aat gcg ccc  ttt ctt atg ttc atc  ggc tct     52301 Pro Phe Ser  Leu Phe Asn Ala Pro  Phe Leu Met Phe Ile  Gly Ser                   1615                 1620                 1625                   gcg aga aga  ttc ctc gtg aag ttt  gtg cga ccc ttt gac  ata tgc     52346 Ala Arg Arg  Phe Leu Val Lys Phe  Val Arg Pro Phe Asp  Ile Cys                   1630                 1635                 1640                   cgg tgt aaa  atc ctc gtc aga act  ttc tcc ttc cgg ggg  atc gac     52391 Arg Cys Lys  Ile Leu Val Arg Thr  Phe Ser Phe Arg Gly  Ile Asp                   1645                 1650                 1655                   ttc ctt gtc  cca cgt gta act gta  tgt tgg gaa gtg agc  tat atc     52436 Phe Leu Val  Pro Arg Val Thr Val  Cys Trp Glu Val Ser  Tyr Ile                   1660                 1665                 1670                   ctc att atc  tga gca atg tgt ctg ttt  ggc ttc cgc ggt gtc  atg     52481 Leu Ile Ile      Ala Met Cys Leu Phe  Gly Phe Arg Gly Val  Met                   1675                     1680                 1685               ttt atc tct gtg  gct tgt tat gct ttc  tgg cgg gcc ctc gtg  ttc     52526 Phe Ile Ser Val  Ala Cys Tyr Ala Phe  Trp Arg Ala Leu Val  Phe                       1690                 1695                 1700               gct agt ttt acg  tgt tgt tgc cag gtg  gct tcg atc ttc tgg  ttc     52571 Ala Ser Phe Thr  Cys Cys Cys Gln Val  Ala Ser Ile Phe Trp  Phe                       1705                 1710                 1715               cgg gtc tgt ggg  atg ctc aag gtt agt  ctc gtc tgt aat aac  tgc     52616 Arg Val Cys Gly  Met Leu Lys Val Ser  Leu Val Cys Asn Asn  Cys                       1720                 1725                 1730               gaa att atc aga  cgg gtc aga agt tat  tga cgg act ctg aat ttt      52661 Glu Ile Ile Arg  Arg Val Arg Ser Tyr      Arg Thr Leu Asn Phe                        1735                 1740                     1745           cca atc aca agt ttc  tga gag tac ttg ttc gaa  ttc cga taa ttc tga  52709 Pro Ile Thr Ser Phe      Glu Tyr Leu Phe Glu  Phe Arg     Phe                            1750                     1755                            ttc tgt  tcc gct ctg cac tgg  act agc aac aga gta  tag act ggt     52754 Phe Cys  Ser Ala Leu His Trp  Thr Ser Asn Arg Val      Thr Gly               1760                 1765                 1770                       atc ata att  tgg atc cca cag ttc  ggt atc ggt gtc tgt  ttc cgg     52799 Ile Ile Ile  Trp Ile Pro Gln Phe  Gly Ile Gly Val Cys  Phe Arg                   1775                 1780                 1785                   act cga ttc  tgt gcc aga ttg agt  tgg gga ttc act atc  aat agt     52844 Thr Arg Phe  Cys Ala Arg Leu Ser  Trp Gly Phe Thr Ile  Asn Ser                   1790                 1795                 1800                   gcg caa gtt  gtc tgt agg tgt gct  aat ggg cga gct ttc  atg ttg     52889 Ala Gln Val  Val Cys Arg Cys Ala  Asn Gly Arg Ala Phe  Met Leu                   1805                 1810                 1815                   atc tgt ctt  cgc aca tac cgt gct  atg aat tgg gcg cgg  gcg cgt     52934 Ile Cys Leu  Arg Thr Tyr Arg Ala  Met Asn Trp Ala Arg  Ala Arg                   1820                 1825                 1830                   aca ttc acc  att ctc aaa act act  ttt ttc ttc aga agc  agt att     52979 Thr Phe Thr  Ile Leu Lys Thr Thr  Phe Phe Phe Arg Ser  Ser Ile                   1835                 1840                 1845                   gtg ctc ttt  ctc act ttc gga tga atc  gct agg aag agg aat  ttt     53024 Val Leu Phe  Leu Thr Phe Gly     Ile  Ala Arg Lys Arg Asn  Phe                   1850                     1855                 1860               gga agc atc tgg  ata cct tac agg aga  ttt aga aat ctc ggt  agt     53069 Gly Ser Ile Trp  Ile Pro Tyr Arg Arg  Phe Arg Asn Leu Gly  Ser                       1865                 1870                 1875               tat gac atg ttc  atc aga tga gga aga tag caa  gcg tga cgg tgt ttt  53117 Tyr Asp Met Phe  Ile Arg     Gly Arg     Gln  Ala     Arg Cys Phe                    1880                         1885                            aca  cga ttc tga agc ttt gaa  ata gcg aga ttt gct  act cct gcg     53162 Thr  Arg Phe     Ser Phe Glu  Ile Ala Arg Phe Ala  Thr Pro Ala           1890                     1895                 1900                       ttt gct  cga gtg ctt tgg atg  ttg gga tga ggc ctt ggt  gtt gcc     53207 Phe Ala  Arg Val Leu Trp Met  Leu Gly     Gly Leu Gly  Val Ala               1905                 1910                     1915                   agg ctc cgg  ttt ctt aaa ggt tat  ccg agg ggg aag ttc  cac gtg     53252 Arg Leu Arg  Phe Leu Lys Gly Tyr  Pro Arg Gly Lys Phe  His Val                   1920                 1925                 1930                   ttc ttt tag ctt  ttc tcc ata ttc cgg  tct acg ggt aca aat  gtc     53297 Phe Phe     Leu  Phe Ser Ile Phe Arg  Ser Thr Gly Thr Asn  Val                       1935                 1940                 1945               ctc tgg atc ttc  gaa tat tgg gac atc  tcc ccc tac tac atc  gct     53342 Leu Trp Ile Phe  Glu Tyr Trp Asp Ile  Ser Pro Tyr Tyr Ile  Ala                       1950                 1955                 1960               ata aaa tat atc  ccc agg atc ccc gag  att aga cga gaa cgt  ccc     53387 Ile Lys Tyr Ile  Pro Arg Ile Pro Glu  Ile Arg Arg Glu Arg  Pro                       1965                 1970                 1975               cgt gtc tga aaa ttt  tac tgg gag gtc ctc  ata ttt ggg gtc tcc      53432 Arg Val     Lys Phe  Tyr Trp Glu Val Leu  Ile Phe Gly Val Ser                            1980                 1985                 1990           atg gag cct taa aac gaa  tgg cga tgg agc tgt  ttc gag aga aac taa  53480 Met Glu Pro     Asn Glu  Trp Arg Trp Ser Cys  Phe Glu Arg Asn                                1995                 2000                            tat  ctg tgt tat ttt gtt  tcc cct gtc gat ggc  aaa aag taa cga     53525 Tyr  Leu Cys Tyr Phe Val  Ser Pro Val Asp Gly  Lys Lys     Arg           2005                 2010                 2015                           cgg agc  atg ctt gag ggg ttt  gct gga cag tac ggt  tga taa ctc     53570 Arg Ser  Met Leu Glu Gly Phe  Ala Gly Gln Tyr Gly          Leu               2020                 2025                 2030                       tgc tat ctg gga  ggc cag aca ggc gtt  ctc tat ggg gtt ggc  atc     53615 Cys Tyr Leu Gly  Gly Gln Thr Gly Val  Leu Tyr Gly Val Gly  Ile                       2035                 2040                 2045               acc ggc aat aat  gtc tcc caa ttg aat  tgc cgt ctt ggc agt  agt     53660 Thr Gly Asn Asn  Val Ser Gln Leu Asn  Cys Arg Leu Gly Ser  Ser                       2050                 2055                 2060               aga gga cag cgc  act ttc taa tgg att tgt  ttt ccg tcc atc gaa      53705 Arg Gly Gln Arg  Thr Phe     Trp Ile Cys  Phe Pro Ser Ile Glu                        2065                     2070                 2075           aat agt aga aaa caa  aac caa acc gtt att  tga gct tag ttc ccc tgc  53753 Asn Ser Arg Lys Gln  Asn Gln Thr Val Ile      Ala     Phe Pro Cys                        2080                 2085                                ttc  ata tgc gat gac gac  agg tgc tcc aaa aag  aag tga aat aat     53798 Phe  Ile Cys Asp Asp Asp  Arg Cys Ser Lys Lys  Lys     Asn Asn           2090                 2095                 2100                           tgc gat  atc aaa agc ggt taa gtc  cca tat gga ttt atg  ggg ctc     53843 Cys Asp  Ile Lys Ser Gly     Val  Pro Tyr Gly Phe Met  Gly Leu               2105                     2110                 2115                   ctc ccg aag  gga ata tat ccc agc  acg atc tac ggt ccc  gaa caa     53888 Leu Pro Lys  Gly Ile Tyr Pro Ser  Thr Ile Tyr Gly Pro  Glu Gln                   2120                 2125                 2130                   ttt gtc atc  agg ggc ctc gaa gac  tcg gct gag atc act  gtt tgg     53933 Phe Val Ile  Arg Gly Leu Glu Asp  Ser Ala Glu Ile Thr  Val Trp                   2135                 2140                 2145                   gga aaa gtc  ata atc gca gct tgg  cga tga gcg gga aac cat  ata     53978 Gly Lys Val  Ile Ile Ala Ala Trp  Arg     Ala Gly Asn His  Ile                   2150                 2155                     2160               atg aat cat ggt  atc gat ggg ctc tcc  atc aat atc gtc atg  ggg     54023 Met Asn His Gly  Ile Asp Gly Leu Ser  Ile Asn Ile Val Met  Gly                       2165                 2170                 2175               tcc cat gga aac  cca tag tgc ctc cag gat  att cct cgg gat gca      54068 Ser His Gly Asn  Pro     Cys Leu Gln Asp  Ile Pro Arg Asp Ala                        2180                     2185                 2190           ttt gag ggc aag aat  cat tag aaa ggc act tgt  ctc ata ttc ttc      54113 Phe Glu Gly Lys Asn  His     Lys Gly Thr Cys  Leu Ile Phe Phe                            2195                     2200                            tcc  gga aca aaa ttt ttc  aag cat gca tat ccc  tga cct gac aga     54158 Ser  Gly Thr Lys Phe Phe  Lys His Ala Tyr Pro      Pro Asp Arg           2205                 2210                 2215                           ggg aaa  tct ttt cca ggg gtc  taa aaa tct caa aat aga  gga ggg     54203 Gly Lys  Ser Phe Pro Gly Val      Lys Ser Gln Asn Arg  Gly Gly               2220                 2225                     2230                   cgg agg ttg  gga aag cca gtc ttt  agg gtc tat cac aaa  aca tgc     54248 Arg Arg Leu  Gly Lys Pro Val Phe  Arg Val Tyr His Lys  Thr Cys                   2235                 2240                 2245                   ccc agc tga agg  atc gaa atg ctg cct  acc ttt tga tag agg gaa      54293 Pro Ser     Arg  Ile Glu Met Leu Pro  Thr Phe         Arg Glu                        2250                 2255                                    aga  gag tgc ttt ttt att  ctg agt ttt tct ttg  gta agg atc aag     54338 Arg  Glu Cys Phe Phe Ile  Leu Ser Phe Ser Leu  Val Arg Ile Lys           2260                 2265                 2270                           aga  tgt tat tcc tac ctt  ctc gcg cac tgc cct  aga taa aac aac     54383 Arg  Cys Tyr Ser Tyr Leu  Leu Ala His Cys Pro  Arg     Asn Asn           2275                 2280                 2285                           atc tag ggt  att tac tat gta ttc  agt ttg ctt taa ata att  aag     54428 Ile     Gly  Ile Tyr Tyr Val Phe  Ser Leu Leu     Ile Ile  Lys                   2290                 2295                     2300               cct gcc aaa gta  aac tag gtg tgc agg ttc  gca tgc agt taa aag      54473 Pro Ala Lys Val  Asn     Val Cys Arg Phe  Ala Cys Ser     Lys                        2305                     2310                                gat  taa aat atc aat agc ggt  tat tga gta aat gcg aac  tgc ttt     54518 Asp      Asn Ile Asn Ser Gly  Tyr     Val Asn Ala Asn  Cys Phe           2315                     2320                     2325                   gtc gaa ttg  att ttt ctg att gat  ctt atc cag gtc tgt  gtg ggg     54563 Val Glu Leu  Ile Phe Leu Ile Asp  Leu Ile Gln Val Cys  Val Gly                   2330                 2335                 2340                   tat aaa gtt  gcc cat ctc atc tat  tat tac atc ccg cgc  tga ccc     54608 Tyr Lys Val  Ala His Leu Ile Tyr  Tyr Tyr Ile Pro Arg      Pro                   2345                 2350                 2355                   taa agt ttt tga cag agt  cag ata agt cag ggc  act gta aat tgt      54653 Ser Phe     Gln Ser  Gln Ile Ser Gln Gly  Thr Val Asn Cys                            2360                 2365                              aca  tgc ggc gac cat cgg  agt atc tgt ttg ttt  gtt cga gct tgg     54698 Thr  Cys Gly Asp His Arg  Ser Ile Cys Leu Phe  Val Arg Ala Trp           2370                 2375                 2380                           cca  ggt tcg ggt gga aca  gaa aat ggt gta aga  atg gaa gcc cgc     54743 Pro  Gly Ser Gly Gly Thr  Glu Asn Gly Val Arg  Met Glu Ala Arg           2385                 2390                 2395                           ttc  tat atc gct agt cct  ggc tct cat cca tag agt  tgc cgc gac     54788 Phe  Tyr Ile Ala Ser Pro  Gly Ser His Pro     Ser  Cys Arg Asp           2400                 2405                     2410                       caa tga gag  ggc tgc att ttg tct  gac gga taa ttt ttt tac  taa     54833 Gln     Glu  Gly Cys Ile Leu Ser  Asp Gly     Phe Phe Tyr                        2415                 2420                     2425               ctc gtt gtc ttt tgg  att ctc aag ttc caa  gga gag agg ctc act      54878 Leu Val Val Phe Trp  Ile Leu Lys Phe Gln  Gly Glu Arg Leu Thr                            2430                 2435                 2440           ttg gtc cga aga tgt  gca taa cgc agc tga cca caa  ggc ggc agc ttt  54926 Leu Val Arg Arg Cys  Ala     Arg Ser     Pro Gln  Gly Gly Ser Phe                        2445                         2450                        tgc  agt ggc cgt tcg ata  aca aag agt gta ttc  cag ggg cat tcc     54971 Cys  Ser Gly Arg Ser Ile  Thr Lys Ser Val Phe  Gln Gly His Ser           2455                 2460                 2465                           gtt  atc tgt gac cat gac  cga gcc tat tgt tcc  gag gtg gtg aaa     55016 Val  Ile Cys Asp His Asp  Arg Ala Tyr Cys Ser  Glu Val Val Lys           2470                 2475                 2480                           cgt  aac cgt cat aac cgc  ttt ttt att ctg tct  cat ata gct gga     55061 Arg  Asn Arg His Asn Arg  Phe Phe Ile Leu Ser  His Ile Ala Gly           2485                 2490                 2495                           agc  cct ggc gag ttc ccg  gac tct gga aga aaa  ggg agt ctc cgc     55106 Ser  Pro Gly Glu Phe Pro  Asp Ser Gly Arg Lys  Gly Ser Leu Arg           2500                 2505                 2510                           aga  aga tag tct cgg gat gat  gtc gtc tga aaa agc tat  acc caa     55151 Arg  Arg     Ser Arg Asp Asp  Val Val     Lys Ser Tyr  Thr Gln                2515                     2520                     2525              ggc ctt gta  agc aag gta aag ttc  aag tct tag cag tat taa ctt      55196 Gly Leu Val  Ser Lys Val Lys Phe  Lys Ser     Gln Tyr     Leu                    2530                 2535                         2540           ttt aat atc ttc ata  ctt tgc tcg gta cca  ctt tgg aac aga ctc      55241 Phe Asn Ile Phe Ile  Leu Cys Ser Val Pro  Leu Trp Asn Arg Leu                            2545                 2550                 2555           aaa aat tgc agt taa ttc  taa atg gag aga ttt caa  agc ttt gaa atg  55289 Lys Asn Cys Ser     Phe      Met Glu Arg Phe Gln  Ser Phe Glu Met                            2560                     2565                        cat  ctc tgt ctt tgc ttt  aaa gat tac ttc gtc  gaa tgt tcg cat     55334 His  Leu Cys Leu Cys Phe  Lys Asp Tyr Phe Val  Glu Cys Ser His           2570                 2575                 2580                           ggc  att agt cag tgt gat  gat gtt ttc ttc tgc  ctt ttg taa tat     55379 Gly  Ile Ser Gln Cys Asp  Asp Val Phe Phe Cys  Leu Leu     Tyr           2585                 2590                 2595                           ggg agt  ata ttc tga aat gtc ttt  ttg ata ctt ctt tat  ttc cgg     55424 Gly Ser  Ile Phe     Asn Val Phe  Leu Ile Leu Leu Tyr  Phe Arg               2600                     2605                 2610                   agt tga tgt aac  ttg agc ctc tac cat  ttg caa ccg gct ggc  taa     55469 Ser     Cys Asn  Leu Ser Leu Tyr His  Leu Gln Pro Ala Gly                            2615                 2620                 2625               gcg ttc tcg tga ttc ctt  tag att gtg aac tgc att  gac gta tgc acc  55517 Ala Phe Ser     Phe Leu      Ile Val Asn Cys Ile  Asp Val Cys Thr                            2630                     2635                        cca  tga ctc ttc gct ggt gtc  ttt ttc ttt tcc gga  ggt ttc ctt     55562 Pro      Leu Phe Ala Gly Val  Phe Phe Phe Ser Gly  Gly Phe Leu           2640                     2645                 2650                       aaa gga  agt ggc agc tga taa aaa ata  ctt ata gta agc ttc  gag     55607 Lys Gly  Ser Gly Ser         Lys Ile  Leu Ile Val Ser Phe  Glu               2655                         2660                 2665               atc ttt cag acc  cgc gtc agc ctc ttc  ata cag tcg ttt aag  ttc     55652 Ile Phe Gln Thr  Arg Val Ser Leu Phe  Ile Gln Ser Phe Lys  Phe                       2670                 2675                 2680               atc caa tcg ctt  ttt aaa atc ttc taa tgg  tcc gcg ttc atc gat      55697 Ile Gln Ser Leu  Phe Lys Ile Phe     Trp  Ser Ala Phe Ile Asp                        2685                     2690                 2695           aga gtt tac tag ttc aca  tgt atc gag aat gtg  agc cga aga aaa      55742 Arg Val Tyr     Phe Thr  Cys Ile Glu Asn Val  Ser Arg Arg Lys                                2700                 2705                            cat  cca ttc caa tga ttt ggg  gtc cac gga agt tat  acc ggt gga     55787 His  Pro Phe Gln     Phe Gly  Val His Gly Ser Tyr  Thr Gly Gly           2710                     2715                 2720                       att gct  ttt ctc ttc cac ttc  gcg aaa gag att aag  gaa aag gat     55832 Ile Ala  Phe Leu Phe His Phe  Ala Lys Glu Ile Lys  Glu Lys Asp               2725                 2730                 2735                       aat tat  atc ttc aag tga ttc tgc  cct ttc gat gcc cgg  ggc aac     55877 Asn Tyr  Ile Phe Lys     Phe Cys  Pro Phe Asp Ala Arg  Gly Asn               2740                     2745                 2750                   ttc ctt ggg  cac gcg acc caa tag taa ccc  ttt cag ctt ttt taa      55922 Phe Leu Gly  His Ala Thr Gln         Pro  Phe Gln Leu Phe                        2755                         2760                                gcc  ctt ctg cat acg caa  gac ttc ctc ttc tct  tac act cca taa     55967 Ala  Leu Leu His Thr Gln  Asp Phe Leu Phe Ser  Tyr Thr Pro               2765                 2770                 2775                           ttg ctc  cag cat gtt ttt aat  ttt gcc gtc tgt gtt  ctt ttt ttg     56012 Leu Leu  Gln His Val Phe Asn  Phe Ala Val Cys Val  Leu Phe Leu               2780                 2785                 2790                       ttg ttc  cgc ctt gtt aat ttc  tgc gga taa aaa taa ttt aac  atc     56057 Leu Phe  Arg Leu Val Asn Phe  Cys Gly     Lys     Phe Asn  Ile               2795                 2800                         2805               tcg caa cgc cct  gtg aga agc atc ttg  ata ggc ggt atg gtc  aaa     56102 Ser Gln Arg Pro  Val Arg Ser Ile Leu  Ile Gly Gly Met Val  Lys                       2810                 2815                 2820               cac agt gac atc  atc gga gtg tag act gtc  tgg cag ctc ctt gag      56147 His Ser Asp Ile  Ile Gly Val     Thr Val  Trp Gln Leu Leu Glu                        2825                     2830                 2835           tgc gat gag ggc cgt  ggg tat cac ttc gcc  gcg gtc taa ccg ctt      56192 Cys Asp Glu Gly Arg  Gly Tyr His Phe Ala  Ala Val     Pro Leu                            2840                 2845                                ttt  ggc att ttc tgg act  tct ctc tat ctg tgc  act ctc ttc agc     56237 Phe  Gly Ile Phe Trp Thr  Ser Leu Tyr Leu Cys  Thr Leu Phe Ser           2850                 2855                 2860                           taa ttg  aag ctc ctt ctt taa ctc  atc ttg caa cag gat  tgc ttt     56282 Leu  Lys Leu Leu Leu     Leu  Ile Leu Gln Gln Asp  Cys Phe           2865                     2870                 2875                   ttc ttt aat  agt ttg aat ctt tga tga aaa  ttc gat gaa ttg ttc      56327 Phe Phe Asn  Ser Leu Asn Leu         Lys  Phe Asp Glu Leu Phe                    2880                         2885                 2890           atg cgc ttg caa gta  aaa gtc aac aaa ttc  tga taa ttc agg aat gcg  56375 Met Arg Leu Gln Val  Lys Val Asn Lys Phe          Phe Arg Asn Ala                        2895                 2900                                cat  caa ttg ctc gga cag  tcg gaa aac tac gcc  cct ggt atc aat     56420 His  Gln Leu Leu Gly Gln  Ser Glu Asn Tyr Ala  Pro Gly Ile Asn           2905                 2910                 2915                           ttg  aga ccc ttc tac ggt  aac ttc tgc cgt ttc  caa aat atc ctt     56465 Leu  Arg Pro Phe Tyr Gly  Asn Phe Cys Arg Phe  Gln Asn Ile Leu           2920                 2925                 2930                           tgc  aat tgc aca aac ggt  cgt cag gcc ttc tac  ctt tgc tcc aaa     56510 Cys  Asn Cys Thr Asn Gly  Arg Gln Ala Phe Tyr  Leu Cys Ser Lys           2935                 2940                 2945                           taa agt  ttc ata gta ttt act  gac caa cgg gaa tga atg  tac cca     56555 Ser  Phe Ile Val Phe Thr  Asp Gln Arg Glu     Met  Tyr Pro           2950                 2955                     2960                   cgt aga agc  ggc tat agg agc tac  ggc tat ttt gtt tcc  ctg ccc     56600 Arg Arg Ser  Gly Tyr Arg Ser Tyr  Gly Tyr Phe Val Ser  Leu Pro                   2965                 2970                 2975                   tgc ttc tgc  att acc aga ata tgg  gtt gaa gga gaa aaa  cga tcg     56645 Cys Phe Cys  Ile Thr Arg Ile Trp  Val Glu Gly Glu Lys  Arg Ser                   2980                 2985                 2990                   aag aag ttt  tgt gcc ggt tgc gag  cca gtc att tac aac  gtt ttg     56690 Lys Lys Phe  Cys Ala Gly Cys Glu  Pro Val Ile Tyr Asn  Val Leu                   2995                 3000                 3005                   agc tag atg ttt  tag ttc tgt gtg ggc ttt  atg tgt act att aac      56735 Ser     Met Phe      Phe Cys Val Gly Phe  Met Cys Thr Ile Asn                        3010                     3015                 3020           atg tgg tcg gag atc  ata tat cgt ttc cca  atc gtc tgc cga aaa      56780 Met Trp Ser Glu Ile  Ile Tyr Arg Phe Pro  Ile Val Cys Arg Lys                            3025                 3030                 3035           ctt agc act gct acg  atc ttg gag gag tgc  agc ctt cgt tgc ttc      56825 Leu Ser Thr Ala Thr  Ile Leu Glu Glu Cys  Ser Leu Arg Cys Phe                            3040                 3045                 3050           cag cca ctc gat ttc  ctc tat ggc aag tat  ggc att atg cgc agc      56870 Gln Pro Leu Asp Phe  Leu Tyr Gly Lys Tyr  Gly Ile Met Arg Ser                            3055                 3060                 3065           ctc gga aag gtt gct  aca tgc ttt ttc tag aga  taa taa ttc cgg gga  56918 Leu Gly Lys Val Ala  Thr Cys Phe Phe     Arg          Phe Arg Gly                        3070                     3075                            aaa tct  ttc ctc att ggt aag  atc taa ctg tgc cac ctc  ttt ctt     56963 Lys Ser  Phe Leu Ile Gly Lys  Ile     Leu Cys His Leu  Phe Leu               3080                 3085                     3090                   aac cct ttc  cac cag cac tgt agc  cga gtc tgc tat ttg  tcg atc     57008 Asn Pro Phe  His Gln His Cys Ser  Arg Val Cys Tyr Leu  Ser Ile                   3095                 3100                 3105                   acg taa ttc tgg  gac att cag ttt ttc  cgt ggc gtt caa aat  gtc     57053 Thr     Phe Trp  Asp Ile Gln Phe Phe  Arg Gly Val Gln Asn  Val                       3110                 3115                 3120               ttt agt cag tct  ttc aat aac atc cga  tag ctg ttt tct cct tgc      57098 Phe Ser Gln Ser  Phe Asn Asn Ile Arg      Leu Phe Ser Pro Cys                        3125                 3130                     3135           ccg ttg ata agc ttc  aga ctc cag ctc gta  gag att atc acg gat      57143 Pro Leu Ile Ser Phe  Arg Leu Gln Leu Val  Glu Ile Ile Thr Asp                            3140                 3145                 3150           gct aaa tag aaa ctg agg  tat ttg cga agc ccc  tct gtt att ttg      57188 Ala Lys     Lys Leu Arg  Tyr Leu Arg Ser Pro  Ser Val Ile Leu                                3155                 3160                            cac  atc atg aat aac tgc  ctc cac ctc ttg tct  ggc gcg aga gaa     57233 His  Ile Met Asn Asn Cys  Leu His Leu Leu Ser  Gly Ala Arg Glu           3165                 3170                 3175                           ccc  atc cgc atc tcc att  tgc ttt cgc cgt ctc  tat gac gaa cag     57278 Pro  Ile Arg Ile Ser Ile  Cys Phe Arg Arg Leu  Tyr Asp Glu Gln           3180                 3185                 3190                           aag  ccg ttc tgc atg atc  gag aaa gac ttc aaa  cgc tct gtt cag     57323 Lys  Pro Phe Cys Met Ile  Glu Lys Asp Phe Lys  Arg Ser Val Gln           3195                 3200                 3205                           tgc  atg ttt gtt ttt tcc  tcc gga aag aag cgt  tgc tgt aaa acg     57368 Cys  Met Phe Val Phe Ser  Ser Gly Lys Lys Arg  Cys Cys Lys Thr           3210                 3215                 3220                           gat  agc gtc gac aag atc  atg caa atc tgt ggt  att tag gga tcc     57413 Asp  Ser Val Asp Lys Ile  Met Gln Ile Cys Gly  Ile     Gly Ser           3225                 3230                 3235                           aaa tgc  acg tcc ata ctc ttc  cag caa gtt ttc gaa  atc gtc ccg     57458 Lys Cys  Thr Ser Ile Leu Phe  Gln Gln Val Phe Glu  Ile Val Pro               3240                 3245                 3250                       tac atg  ctt tcc aat aaa atc  atc att tct agc aag  gtt tat aac     57503 Tyr Met  Leu Ser Asn Lys Ile  Ile Ile Ser Ser Lys  Val Tyr Asn               3255                 3260                 3265                       ttc ctc  ttg ggg aat gtt tct  tgt ctg gag cgc gct  ttt tat att     57548 Phe Leu  Leu Gly Asn Val Ser  Cys Leu Glu Arg Ala  Phe Tyr Ile               3270                 3275                 3280                       tct tag tcc  ttc gaa ttt ttt taa cgg  gcg caa tat ctg cgt  cac     57593 Ser     Ser  Phe Glu Phe Phe     Arg  Ala Gln Tyr Leu Arg  His                   3285                     3290                 3295               ggt acg ttt cgt  ttc ttc gtg cgc agt  ttc ttc ctt tct aat  ctc     57638 Gly Thr Phe Arg  Phe Phe Val Arg Ser  Phe Phe Leu Ser Asn  Leu                       3300                 3305                 3310               ttt gga gaa ttg  atc tat caa acg tga agc  gcg gtc ttc tag cgc      57683 Phe Gly Glu Leu  Ile Tyr Gln Thr     Ser  Ala Val Phe     Arg                        3315                     3320                                gtc  aag gcg acg gct ttg  ttc cac ggt agt tct  cgc ggc ttt ggc     57728 Val  Lys Ala Thr Ala Leu  Phe His Gly Ser Ser  Arg Gly Phe Gly           3325                 3330                 3335                           tac  ttt gat tga ttc ctg tgt  gtg aag gac ata ttg  cag gcg tac     57773 Tyr  Phe Asp     Phe Leu Cys  Val Lys Asp Ile Leu  Gln Ala Tyr           3340                     3345                 3350                       ttg ggg  ggg cgc tgt aat agc  ttc cac tcc ctc gag  ctc gtt ttc     57818 Leu Gly  Gly Arg Cys Asn Ser  Phe His Ser Leu Glu  Leu Val Phe               3355                 3360                 3365                       taa ttc agc  cag cat att ttc agt  tag ccc aaa act ctg agc  aat     57863 Phe Ser  Gln His Ile Phe Ser      Pro Lys Thr Leu Ser  Asn               3370                 3375                     3380                 agc gtt gat gtc  atg aat agc gga gat  cca ttt tgt agg tga tgg      57908 Ser Val Asp Val  Met Asn Ser Gly Asp  Pro Phe Cys Arg     Trp                        3385                 3390                     3395           gac aag tcc ctc tgc  atg agc ttc gaa tag atg  tag gga cca tct ctt  57956 Asp Lys Ser Leu Cys  Met Ser Phe Glu     Met      Gly Pro Ser Leu                        3400                     3405                            gag  ttt ttc atc ggt ggt  aag gtc ttt ccc atc  gat tac cag atc     58001 Glu  Phe Phe Ile Gly Gly  Lys Val Phe Pro Ile  Asp Tyr Gln Ile           3410                 3415                 3420                           tct  gaa aat tct caa tgg  ctt tct tgt gaa cag  tac ttg taa gtt     58046 Ser  Glu Asn Ser Gln Trp  Leu Ser Cys Glu Gln  Tyr Leu     Val           3425                 3430                 3435                           ctt cga  tgg gtt tcc tgc aag  ttg cct aat caa ctg  aaa ccg cct     58091 Leu Arg  Trp Val Ser Cys Lys  Leu Pro Asn Gln Leu  Lys Pro Pro               3440                 3445                 3450                       ttc aat  ctc ctc tat gtt ttt  tag cca att gac agc att  ctc tac     58136 Phe Asn  Leu Leu Tyr Val Phe      Pro Ile Asp Ser Ile  Leu Tyr               3455                 3460                     3465                   aaa tag agt agg  gat gct aaa cct tcc  caa tac tcc acc gac  ccc     58181 Lys     Ser Arg  Asp Ala Lys Pro Ser  Gln Tyr Ser Thr Asp  Pro                       3470                 3475                 3480               aga tga taa tgc cga tat  gct ata gtc aat ggc  gcc cgc ata ttt      58226 Arg         Cys Arg Tyr  Ala Ile Val Asn Gly  Ala Arg Ile Phe                                3485                 3490                            ctt  tag gaa ttt gac cgc agc  atc ctc aat tct gat  aat tgc gtc     58271 Leu      Glu Phe Asp Arg Ser  Ile Leu Asn Ser Asp  Asn Cys Val           3495                     3500                 3505                       gtc gag  ctt tcg att aat atc  ctg aac gtc gag tcc  tgt aaa ttt     58316 Val Glu  Leu Ser Ile Asn Ile  Leu Asn Val Glu Ser  Cys Lys Phe               3510                 3515                 3520                       cct caa  acc ctg tat aat tgc  ttg tgg tat gtc cgg  agt ttg ggt     58361 Pro Gln  Thr Leu Tyr Asn Cys  Leu Trp Tyr Val Arg  Ser Leu Gly               3525                 3530                 3535                       tgc ttg  gat att gat cac atc  tac tcc tat gtt aca  aat ttc atc     58406 Cys Leu  Asp Ile Asp His Ile  Tyr Ser Tyr Val Thr  Asn Phe Ile               3540                 3545                 3550                       aat gac  tgt cca cgt att ggc  gag aag tga tag tac tgc aat  ttt     58451 Asn Asp  Cys Pro Arg Ile Gly  Glu Lys         Tyr Cys Asn  Phe               3555                 3560                         3565               ggc atc agc gaa  gcg tgc ttc act ttc  tat cac aga ttt cgc  agc     58496 Gly Ile Ser Glu  Ala Cys Phe Thr Phe  Tyr His Arg Phe Arg  Ser                       3570                 3575                 3580               acg ctc tac tac  atc agt aat ttt gtg  tcc gtg tga att aac atc      58541 Thr Leu Tyr Tyr  Ile Ser Asn Phe Val  Ser Val     Ile Asn Ile                        3585                 3590                     3595           ttc tat tag aat act cgt  ttt att tga aca ttc cag  cag ttc ttc gga  58589 Phe Tyr     Asn Thr Arg  Phe Ile     Thr Phe Gln  Gln Phe Phe Gly                            3600                     3605                        taa ctg  ttc gta cgc agt tgg  aag ttg atc acg ctc  ggc act gtc     58634 Leu  Phe Val Arg Ser Trp  Lys Leu Ile Thr Leu  Gly Thr Val           3610                 3615                 3620                       tgc gat  ttg att caa ttt agc  aat taa cat ttc tgt ctg  cgt taa     58679 Cys Asp  Leu Ile Gln Phe Ser  Asn     His Phe Cys Leu  Arg                   3625                 3630                     3635                   cag gga taa act gac  ctg ctg cgt ttt tct  taa aag aac act gcc      58724 Gln Gly     Thr Asp  Leu Leu Arg Phe Ser      Lys Asn Thr Ala                            3640                 3645                                ccc  ttc act gtt aga gtg  gca tag ctc cag gag caa  gtg ttt ctt     58769 Pro  Phe Thr Val Arg Val  Ala     Leu Gln Glu Gln  Val Phe Leu           3650                 3655                     3660                       ttc gat  aag atc ctg ata acg  cag tct tgt agt ggt  caa tat att     58814 Phe Asp  Lys Ile Leu Ile Thr  Gln Ser Cys Ser Gly  Gln Tyr Ile               3665                 3670                 3675                       gct gat  att ttt ctc tcc gat  gcg agc caa tag cgt tgc  caa tgg     58859 Ala Asp  Ile Phe Leu Ser Asp  Ala Ser Gln     Arg Cys  Gln Trp               3680                 3685                     3690                   gtc tat tag aag  gtc cac cgt gct ttc  caa gtt ttt tgt gga  gac     58904 Val Tyr     Lys  Val His Arg Ala Phe  Gln Val Phe Cys Gly  Asp                       3695                 3700                 3705               ccc gtt ctc tat  aat aaa aaa taa tat gcg  att gca ggt aat tct      58949 Pro Val Leu Tyr  Asn Lys Lys     Tyr Ala  Ile Ala Gly Asn Ser                        3710                     3715                 3720           cac ctt ttc ccg taa aat  ccc aga tcg caa aaa  tct cgt cag tgc      58994 His Leu Phe Pro     Asn  Pro Arg Ser Gln Lys  Ser Arg Gln Cys                                3725                 3730                            cgg  ttc gaa tgt ctc ctg  tgg ggc cgg taa cca ctt  aag agg atc     59039 Arg  Phe Glu Cys Leu Leu  Trp Gly Arg     Pro Leu  Lys Arg Ile           3735                 3740                     3745                       ttc caa  ggc att ttt tat tgg  tgt tat gga tcc taa ttc  aat tgt     59084 Phe Gln  Gly Ile Phe Tyr Trp  Cys Tyr Gly Ser     Phe  Asn Cys               3750                 3755                     3760                   tgc tga tgg gtt  tgt gtc ttc tgc cgt  aag taa att cca aga tag      59129 Cys     Trp Val  Cys Val Phe Cys Arg  Lys     Ile Pro Arg                            3765                 3770                                    ctt  cct cct ggc gct atg  cgg ctt agt att gct  ttg agc ttc tgg     59174 Leu  Pro Pro Gly Ala Met  Arg Leu Ser Ile Ala  Leu Ser Phe Trp           3775                 3780                 3785                           gtc  tga acg tac gga gtg ttt  tct ttg ttt tcg tgg  tgt gag gtt     59219 Val      Thr Tyr Gly Val Phe  Ser Leu Phe Ser Trp  Cys Glu Val           3790                     3795                 3800                       tgt cag  ttt ttc tga gct tcc gat  ttg atc agg att ctt  tgg ctg     59264 Cys Gln  Phe Phe     Ala Ser Asp  Leu Ile Arg Ile Leu  Trp Leu               3805                     3810                 3815                   tgg ctt ctg  tct ctt ttt tga tct tgg  tct tac cgg aac gct  cca     59309 Trp Leu Leu  Ser Leu Phe     Ser Trp  Ser Tyr Arg Asn Ala  Pro                   3820                     3825                 3830               tgg agc aat agc  ctt tct acg gtc cgc  gag ttc aaa aag gtt  ctt     59354 Trp Ser Asn Ser  Leu Ser Thr Val Arg  Glu Phe Lys Lys Val  Leu                       3835                 3840                 3845               ctg aga aaa ctc  tgg gtt tag tcc gag agc  ata gac atg cgc cgg      59399 Leu Arg Lys Leu  Trp Val     Ser Glu Ser  Ile Asp Met Arg Arg                        3850                     3855                 3860           aaa tgg atg aat tat  ttg tgt gct taa cgt aac  act gct taa ttc agc  59447 Lys Trp Met Asn Tyr  Leu Cys Ala     Arg Asn  Thr Ala     Phe Ser                        3865                     3870                            atc  tga tgc gcc ata tga tgc cat  tac tga tgc cgc aat gat  ttc     59492 Ile      Cys Ala Ile     Cys His  Tyr     Cys Arg Asn Asp  Phe           3875                         3880                     3885               ctg gag cgt ttt  ctc cga tac ctt ttc  ttc gtc cca ccc atc  cgc     59537 Leu Glu Arg Phe  Leu Arg Tyr Leu Phe  Phe Val Pro Pro Ile  Arg                       3890                 3895                 3900               att gaa cca gta  gag aaa att cat atc  tat tcc aac tcc aga  cat     59582 Ile Glu Pro Val  Glu Lys Ile His Ile  Tyr Ser Asn Ser Arg  His                       3905                 3910                 3915               att ctt tac ata  tgc ccg aac aaa ttc  tgg gag gtc atc aat  tct     59627 Ile Leu Tyr Ile  Cys Pro Asn Lys Phe  Trp Glu Val Ile Asn  Ser                       3920                 3925                 3930               cct tag ctt gca tac  ata agc act tga cat ttc  cca tcc atg tga atc  59675 Pro     Leu Ala Tyr  Ile Ser Thr     His Phe  Pro Ser Met     Ile                        3935                     3940                            aaa  aat gta cac agg atc  gtc ctt tcc ccc aga  aca cac ggc tgc     59720 Lys  Asn Val His Arg Ile  Val Leu Ser Pro Arg  Thr His Gly Cys           3945                 3950                 3955                           aac  tcc cct gtc acc gat  gat cat tat gaa tcc  caa tcc ttt cct     59765 Asn  Ser Pro Val Thr Asp  Asp His Tyr Glu Ser  Gln Ser Phe Pro           3960                 3965                 3970                           aat  ttt ctt ggc aat gat  ttt ggt aat gcc gtg  tat att cat gga     59810 Asn  Phe Leu Gly Asn Asp  Phe Gly Asn Ala Val  Tyr Ile His Gly           3975                 3980                 3985                           aga  tgg gaa acc ttc gat  tgt gac ttc cat gtc  ata acc cac att     59855 Arg  Trp Glu Thr Phe Asp  Cys Asp Phe His Val  Ile Thr His Ile           3990                 3995                 4000                           ccc  gta tgt tat aga agg  tat cat gag cat gtc  agg ttc atc cca     59900 Pro  Val Cys Tyr Arg Arg  Tyr His Glu His Val  Arg Phe Ile Pro           4005                 4010                 4015                           tga tga tct  cca gag tct ggg aag  ttc gtg aca atc aat  cat tcg     59945 Ser  Pro Glu Ser Gly Lys  Phe Val Thr Ile Asn  His Ser             4020                 4025                 4030                     aaa cga aca  ttt ttt tga ctt gtc ctc  cac ctt ggc ctt ccc  tgt     59990 Lys Arg Thr  Phe Phe     Leu Val Leu  His Leu Gly Leu Pro  Cys                   4035                     4040                 4045               tct cgt aat ctc  cgc ccc ctg gtc taa aat  cgc atc caa act gtc      60035 Ser Arg Asn Leu  Arg Pro Leu Val     Asn  Arg Ile Gln Thr Val                        4050                     4055                 4060           cgc aga gag cat cga  atc gat ccc gta gca  ttc gca ccc ccg cag      60080 Arg Arg Glu His Arg  Ile Asp Pro Val Ala  Phe Ala Pro Pro Gln                            4065                 4070                 4075           gaa ggc ggc gga cgt  ctc cat aca cat ata  gta tct aac agg ttc      60125 Glu Gly Gly Gly Arg  Leu His Thr His Ile  Val Ser Asn Arg Phe                            4080                 4085                 4090           ata ttt tcg agc aaa  ttg gct ggt cga cgc  ggc tac aat aaa ttc      60170 Ile Phe Ser Ser Lys  Leu Ala Gly Arg Arg  Gly Tyr Asn Lys Phe                            4095                 4100                 4105           tcc gaa tgc cgg tgg  taa tct tgt cgg agg atg  gaa atc ctg agc      60215 Ser Glu Cys Arg Trp      Ser Cys Arg Arg Met  Glu Ile Leu Ser                            4110                     4115                            cat  gttcgtgacc tgctagaact gtacatcagt ctgcttctgg ggccttttat        60268 His                                                                      4120                                                                     ttgtttggga agagtttcgt aaatgtctag ccaaccgcac acaagaacgc gataaataca  60328 caccactcaa ttactcatta gtctttct tta ttc aaa cat ttt  tac att tgc    60380                                Leu Phe Lys His Phe  Tyr Ile Cys                                                         4125                      act ttc  caa aac tcg atc gga  aac tgt cag aag agt  ctc tga tga     60425 Thr Phe  Gln Asn Ser Ile Gly  Asn Cys Gln Lys Ser  Leu                       4130                 4135                 4140                       gat tag ttt gtc tgg  aag tgt gtc tac aca  ccc att gtc tat ccc      60470 Asp     Phe Val Trp  Lys Cys Val Tyr Thr  Pro Ile Val Tyr Pro                            4145                 4150                 4155           ttc ttt tgt cga gtg  tcg aac ata gag cgg  ggc ttt gtt ggt ttt      60515 Phe Phe Cys Arg Val  Ser Asn Ile Glu Arg  Gly Phe Val Gly Phe                            4160                 4165                 4170           atc cca cga agt aat  gtc tga cgc ctc ggt aat  aaa ttg att tga tga  60563 Ile Pro Arg Ser Asn  Val     Arg Leu Gly Asn  Lys Leu Ile                                4175                     4180                            tgt atc  ttg ata tcc cct ggc  atc agg cgg ccc taa aag  agt aag     60608 Cys Ile  Leu Ile Ser Pro Gly  Ile Arg Arg Pro     Lys  Ser Lys               4185                 4190                     4195                   agc tga cgc tgt  atc ttc ttt aga agg  ggc aga aat ttc aat  ttc     60653 Ser     Arg Cys  Ile Phe Phe Arg Arg  Gly Arg Asn Phe Asn  Phe                       4200                 4205                 4210               ttc caa ttt tcc  ttc aag ggt cgt ggt  ccc tcc ctc ctc tat  atc     60698 Phe Gln Phe Ser  Phe Lys Gly Arg Gly  Pro Ser Leu Leu Tyr  Ile                       4215                 4220                 4225               tag cag agc tga tgc cgc  gcc aat aac ttt aac  tac atc ttc agg      60743 Gln Ser     Cys Arg  Ala Asn Asn Phe Asn  Tyr Ile Phe Arg                            4230                 4235                              ttt  tgg att aga tgt ctc  gag agc agc ttc att  atc tga aaa agt     60788 Phe  Trp Ile Arg Cys Leu  Glu Ser Ser Phe Ile  Ile     Lys Ser           4240                 4245                 4250                           ttt cca  ggc ttt tac cac atc  gcg ggc cct ttt tac  ccc gac gcc     60833 Phe Pro  Gly Phe Tyr His Ile  Ala Gly Pro Phe Tyr  Pro Asp Ala               4255                 4260                 4265                       tgc gag  tga acg aca taa tgt cca cga  acg ctg gac gac agt  att     60878 Cys Glu      Thr Thr     Cys Pro Arg  Thr Leu Asp Asp Ser  Ile               4270                         4275                 4280               cct cgc gtc cgc  tat gcc aga taa ttc ttc  agc tac ttt cct aca      60923 Pro Arg Val Arg  Tyr Ala Arg     Phe Phe  Ser Tyr Phe Pro Thr                        4285                     4290                 4295           aat gtg aga gcg tgt  ttg agc att acc cgc  aag ctg cca gta tgt      60968 Asn Val Arg Ala Cys  Leu Ser Ile Thr Arg  Lys Leu Pro Val Cys                            4300                 4305                 4310           ggt ctc aag tgc atg  cgt ttt aac ctt tgt  gga atc taa tgc ccg      61013 Gly Leu Lys Cys Met  Arg Phe Asn Leu Cys  Gly Ile     Cys Pro                            4315                 4320                                aat  agc ttt ttc tgt gtg  ccg gat tgc agt ttc  tgc aaa att gac     61058 Asn  Ser Phe Phe Cys Val  Pro Asp Cys Ser Phe  Cys Lys Ile Asp           4325                 4330                 4335                           ggc  ttt cca tat ggc ctg  cat tac agt atc gtc  ctt cca gtg tcc     61103 Gly  Phe Pro Tyr Gly Leu  His Tyr Ser Ile Val  Leu Pro Val Ser           4340                 4345                 4350                           tag ttc  ttt ttt gtc acg gca  act tga gaa aga aac att  gcc caa     61148 Phe  Phe Phe Val Thr Ala  Thr     Glu Arg Asn Ile  Ala Gln           4355                 4360                     4365                   aaa atc cgc  att tgc tga ctg tga aag cag  tgt tct tat ggt agc      61193 Lys Ile Arg  Ile Cys     Leu     Lys Gln  Cys Ser Tyr Gly Ser                    4370                         4375                 4380           agt ctt aga aag tag tat  ttg cac tgc agt aga  tat ctg cat aga      61238 Ser Leu Arg Lys     Tyr  Leu His Cys Ser Arg  Tyr Leu His Arg                                4385                 4390                            tgc  gtt att tag agt ctt gga  aac ctc ctc aag gtc  tct cgt tgc     61283 Cys  Val Ile     Ser Leu Gly  Asn Leu Leu Lys Val  Ser Arg Cys           4395                     4400                 4405                       agc ttg  ctc gaa tgc ctg gga  aat gaa ctt tga tct cat  ggt atc     61328 Ser Leu  Leu Glu Cys Leu Gly  Asn Glu Leu     Ser His  Gly Ile               4410                 4415                     4420                   gac ggc agg  gac agg ctg gac tgt  ttt ctt aaa tgt ttc  tgg aag     61373 Asp Gly Arg  Asp Arg Leu Asp Cys  Phe Leu Lys Cys Phe  Trp Lys                   4425                 4430                 4435                     agt gtc gag  gct cct gtc gca tgc  gtc aga aat ttt aag  caa tgc     61418 Ser Val Glu  Ala Pro Val Ala Cys  Val Arg Asn Phe Lys  Gln Cys                   4440                 4445                 4450                   ttt ttc tat  cgc gct tgc act ttc  gat gga tat ctt ttc  tgt cgg     61463 Phe Phe Tyr  Arg Ala Cys Thr Phe  Asp Gly Tyr Leu Phe  Cys Arg                   4455                 4460                 4465                   att ggt ctt  tgt aat aaa tgg gga  gac cgc taa ctg aaa ttc  caa     61508 Ile Gly Leu  Cys Asn Lys Trp Gly  Asp Arg     Leu Lys Phe  Gln                   4470                 4475                     4480               aat tgc tcg cct  gta ctt ctg agt atc  aga cag gtt ttc tga gag      61553 Asn Cys Ser Pro  Val Leu Leu Ser Ile  Arg Gln Val Phe     Glu                        4485                 4490                     4495           ccc aaa atc tct agc  cca cga taa aag ctt tac  cgc gtg ttg tac      61598 Pro Lys Ile Ser Ser  Pro Arg     Lys Leu Tyr  Arg Val Leu Tyr                            4500                     4505                            cgc  gtc aac ggt gaa tgg  ata agt tga tac tgc gta  aac aat gtc     61643 Arg  Val Asn Gly Glu Trp  Ile Ser     Tyr Cys Val  Asn Asn Val           4510                 4515                     4520                       tct caa  tac ggt cgc tac aaa  aag att gga aaa gag  ttg cat atc     61688 Ser Gln  Tyr Gly Arg Tyr Lys  Lys Ile Gly Lys Glu  Leu His Ile               4525                 4530                 4535                       gat ttt  cgg gtc cgt tcc att  gcg cga aca tat ctc  ttc agg ctc     61733 Asp Phe  Arg Val Arg Ser Ile  Ala Arg Thr Tyr Leu  Phe Arg Leu               4540                 4545                 4550                       cgc gcc  ccc aac ttc aat ggc  ctg gac taa ttc tac ggt  ttg ctc     61778 Arg Ala  Pro Asn Phe Asn Gly  Leu Asp     Phe Tyr Gly  Leu Leu               4555                 4560                     4565                   aaa tcc ata  agt gta gga ttg ttc  gtc ggc act cgc ata  ccc ctt     61823 Lys Ser Ile  Ser Val Gly Leu Phe  Val Gly Thr Arg Ile  Pro Leu                   4570                 4575                 4580                   atc tct ggc  ctt ttc cct gcg ttc  gag cca atc caa att  ttc ctg     61868 Ile Ser Gly  Leu Phe Pro Ala Phe  Glu Pro Ile Gln Ile  Phe Leu                   4585                 4590                 4595                   cgt ctt tcc  tcc taa gtc aga tat gag  gcg ttg tac aaa atc  gaa     61913 Arg Leu Ser  Ser     Val Arg Tyr Glu  Ala Leu Tyr Lys Ile  Glu                   4600                     4605                 4610               cac cac caa atc  tcc aag cgc ctc ggc  tcc tcc atc gct ccc  atc     61958 His His Gln Ile  Ser Lys Arg Leu Gly  Ser Ser Ile Ala Pro  Ile                       4615                 4620                 4625               tat aac tga ggc cag  agt gtc cct aat gat  gtt tag agc atc ttc tga  62006 Tyr Asn     Gly Gln  Ser Val Pro Asn Asp  Val     Ser Ile Phe                            4630                 4635                                tgg  ggc tgg ggc aaa tga tcg  tag tgg tag tag ttc gct ttc aga      62051 Trp  Gly Trp Gly Lys     Ser      Trp         Phe Ala Phe Arg            4640                     4645                             4650           cac ccc tac ccg ccg  gag aag atg cga ata  tgg gtc tac aaa atc      62096 His Pro Tyr Pro Pro  Glu Lys Met Arg Ile  Trp Val Tyr Lys Ile                            4655                 4660                 4665           aga tat aag ccc agc  gta atg ccc tga ata ctg  tag cgt ccg cgg att  62144 Arg Tyr Lys Pro Ser  Val Met Pro     Ile Leu      Arg Pro Arg Ile                        4670                     4675                            ata  tgt ata cat tga gtg cgc  agc gag tcc tat aag  tga tgt ata     62189 Ile  Cys Ile His     Val Arg  Ser Glu Ser Tyr Lys      Cys Ile           4680                     4685                 4690                       tac aac ggc  tcc tag gaa atc tcc aat  gat tgt tcc gtc agt  atc     62234 Tyr Asn Gly  Ser     Glu Ile Ser Asn  Asp Cys Ser Val Ser  Ile                   4695                     4700                 4705               ttc acc cca aag  cgc ctc agc ctt ttg  gat ata cag ttc gca  gtt     62279 Phe Thr Pro Lys  Arg Leu Ser Leu Leu  Asp Ile Gln Phe Ala  Val                       4710                 4715                 4720               gct ctt ggt aaa  ccc cct aag cac taa agt  acg aat tac ttc ctc      62324 Ala Leu Gly Lys  Pro Pro Lys His     Ser  Thr Asn Tyr Phe Leu                        4725                     4730                 4735           tgg gcc acc ggt agc  ggc caa gtc cgg caa  acg cgc cat cat atc      62369 Trp Ala Thr Gly Ser  Gly Gln Val Arg Gln  Thr Arg His His Ile                            4740                 4745                 4750           ggc ggt act tgt tgt  ata agc agt tag ggt ctc  ctt ttg ttc ctc      62414 Gly Gly Thr Cys Cys  Ile Ser Ser     Gly Leu  Leu Leu Phe Leu                            4755                     4760                            tgt  caa gtt att agg cgc  gtc ctc gaa aag ctg  tac gat ggt tgg     62459 Cys  Gln Val Ile Arg Arg  Val Leu Glu Lys Leu  Tyr Asp Gly Trp           4765                 4770                 4775                           gac  atc tag ggc gag gcg ttt  aat ggg aat agt taa tag act  atc     62504 Asp  Ile     Gly Glu Ala Phe  Asn Gly Asn Ser         Thr  Ile                4780                     4785                         4790          taa gtc gtg ccg cca  gca aac tac atg tcc  agg aac atg cgt ctt      62549 Val Val Pro Pro  Ala Asn Tyr Met Ser  Arg Asn Met Arg Leu                        4795                 4800                 4805             cgg agg atc tcc taa cac  gag tag tgg gtc tcg act  gta tgc agc att  62597 Arg Arg Ile Ser     His  Glu     Trp Val Ser Thr  Val Cys Ser Ile                            4810                     4815                        ggc  gta cag ttg cga gat  aga aaa gta tct tga gtc  caa atc ttt     62642 Gly  Val Gln Leu Arg Asp  Arg Lys Val Ser     Val  Gln Ile Phe           4820                 4825                     4830                       agc gat  cca tga ctt ggc cga atc  tgt gtc cag atc aaa  ggc agc     62687 Ser Asp  Pro     Leu Gly Arg Ile  Cys Val Gln Ile Lys  Gly Ser               4835                     4840                 4845                   tag cgc cgt ctg  tgg aaa ctt ccg ata  agg ggg ttt tac tgt  agg     62732 Arg Arg Leu  Trp Lys Leu Pro Ile  Arg Gly Phe Tyr Cys  Arg                   4850                 4855                 4860                 ttc aaa ata ctc  tct gac cat aga gag  ggt cat cag cgc ttt  agt     62777 Phe Lys Ile Leu  Ser Asp His Arg Glu  Gly His Gln Arg Phe  Ser                       4865                 4870                 4875               ttt ggg tag ccc ggt  tga agc cgt gat ata att  taa cat tgc atg gta  62825 Phe Gly     Pro Gly      Ser Arg Asp Ile Ile      His Cys Met Val                        4880                     4885                            tcc  cac taa gcc ttg tag ctt tgt  tcc aag ata ctg tat  ccc gtc     62870 Ser  His     Ala Leu     Leu Cys  Ser Lys Ile Leu Tyr  Pro Val           4890                         4895                 4900                   ttg aac cat  ttg gtg gtc gta taa aag  tgg ata att ttc aat  taa     62915 Leu Asn His  Leu Val Val Val     Lys  Trp Ile Ile Phe Asn                        4905                     4910                 4915               agg aga tag gag ttt ggt  tgg ctc gag tcg gtc  tcc gga tgt ctc      62960 Arg Arg     Glu Phe Gly  Trp Leu Glu Ser Val  Ser Gly Cys Leu                                4920                 4925                            gaa  caa ttg tcg ctc gtc  aac tac caa tct tgc  caa act ccc aaa     63005 Glu  Gln Leu Ser Leu Val  Asn Tyr Gln Ser Cys  Gln Thr Pro Lys           4930                 4935                 4940                           ttc  cgt taa cca gcc act aat  tag act cca ata ttc tac  att tgg     63050 Phe  Arg     Pro Ala Thr Asn      Thr Pro Ile Phe Tyr  Ile Trp           4945                     4950                     4955                   atc att taa ccc  agg aaa ttc ttt acg  tac agc tgt ttc tag gtc      63095 Ile Ile     Pro  Arg Lys Phe Phe Thr  Tyr Ser Cys Phe     Val                        4960                 4965                     4970           atg ttg cag tcc agt  aaa tgc ttc tct caa  ata ttg aat tat tct      63140 Met Leu Gln Ser Ser  Lys Cys Phe Ser Gln  Ile Leu Asn Tyr Ser                            4975                 4980                 4985           gaa atc ttc tcc aga  gtt cca tgg aac aga  tat gcg gct aat aag      63185 Glu Ile Phe Ser Arg  Val Pro Trp Asn Arg  Tyr Ala Ala Asn Lys                            4990                 4995                 5000           aga gcg tgc aaa taa ttt  caa tgt aac gtc cgc  tac tgc cgg agg      63230 Arg Ala Cys Lys     Phe  Gln Cys Asn Val Arg  Tyr Cys Arg Arg                                5005                 5010                            cgc  cat tgt gca tgc gag  cat tat agt ttc aac  cat gtt ttt cct     63275 Arg  His Cys Ala Cys Glu  His Tyr Ser Phe Asn  His Val Phe Pro           5015                 5020                 5025                           agc  ttc tac ttt ctc caa  atc ttc aag tgg aat  tct gtt tct gga     63320 Ser  Phe Tyr Phe Leu Gln  Ile Phe Lys Trp Asn  Ser Val Ser Gly           5030                 5035                 5040                           gat  tag aat tcc ggt gag ggc  tga agt aac acc cgg aac  ttt act     63365 Asp      Asn Ser Gly Glu Gly      Ser Asn Thr Arg Asn  Phe Thr           5045                     5050                     5055                   gca gtc ggt  gtg tac agc ttc aag  gca agc ctt gag gct  atc cgt     63410 Ala Val Gly  Val Tyr Ser Phe Lys  Ala Ser Leu Glu Ala  Ile Arg                   5060                 5065                 5070                   gat ggc ctg  cat ggtttcaata gtcgcgtcaa ctcacggaac cagctggtag       63462 Asp Gly Leu  His                                                                 5075                                                             ctttataggc acgcttctga atgctttcac agcacagttt ttttcagaag tgtgattaga  63522 gccagtgttc tccagcttat aataagcgtc agaacttcct ttccgcgctg ttacatcgta  63582 agcatgtccg gattacttgc tcgcaatgga tcgaacgata aaattcgagc gccagaaaga  63642 gggctgagtg gattcgcaaa cactcgcact catccttatt tacaaagtgt acttcctcga  63702 agaactcacc agcataatct gttatcagcc gcgctggggc gcctaacaac tggccggaat  63762 ttttctcatg gtcttggaac gaggccaggg aaattcttta ttggctcggg gccacgtaac  63822 cccgaagaaa caaataatgc aacgcttgga aaggaagata caagggccat cgcatctaaa  63882 attcttctcg atgttgaatc tacgaggaca aatgatgtaa tttctttaac ccaaataact  63942 gtcatagacc tctgccagcc aggggtcgaa gagtctgggt ctctaatgct ctttttgaaa  64002 ggggttaaag acttactcaa gattttggga tcacgacccg cccgcgagca aattgtgaga  64062 gatttagaga ctgcatttat caatttgaac cgcgcctgcg gcttatcggt tactggggag  64122 agtgctgtca aatccttcga gctttcagcg gtgtctttga gttttgtagc tgcggccggc  64182 gcggctcact accgaaataa ttgcggagta gaggcactac gcgcgttcgt aatcgcgaat  64242 tataaggatt ccgccattac cgaaaagctt gccaattttg atgcattgct tctggcaaca  64302 attgaggcac gagcgtaccc ccatttttta ctatctttag gtgggggctt gataaaagct  64362 accgaaagtg aaacagtaac gtgcatgcga ctcttggtca gagggactca cgaagcagca  64422 gctgccggag atctttctga gaaaaaatgt gccgtactcc acttccctgc atgtctgtta  64482 cttgacctcg atgaaggttt ttgcccacca aggacaaaga tcaatgcaga aggcggtgtt  64542 tactttctac atttattatt cctgtactct accgatatgg gacacccgag ctacgaacta  64602 tatgtggcaa aaacatctct ccccgaaagt tgcatgagag acattttaaa tgaaagattt  64662 acacgacgca gaataaataa cactatagct tccttaacac acatgcaagc tcaagaacgc  64722 caagcctttc ccccttttaa tattgcagaa gctgcggttg ccgcgcgaat ggatctggga  64782 cgagaaagat caagacatcc actggatcca gttcaaggtc gtgatcaacg tcttagggat  64842 atgcagataa cgtccactgg aataccagac cattcggctc tagtattcgc cgcatactgc  64902 atgctaggga cggcgggcag tattggcggg gagccttctt tttctactat ccagaaaaac  64962 gggactagtc taaagtatgt tcacattcgc gattttaact ttaggggcgg cccatggaat  65022 gtttgtatgt aacacaccca cttctaaata aatacaagtc tgttcacccc atgtgtctat  65082 tcagttctca ttcgaggcaa gggagcaagg atccctctca ggacaattat aggattatct  65142 cctgagcagc ccggagccat gctttcgttt ttcgatgaac tcgaaagcat tgcccatcta  65202 gaagagtgtc tgcgggacat cgaaacgcgt atgcgaacgt ctgggtttga tcctaaatcc  65262 atcattagag acgattttga cataagggct gggcgcgagg gcctcgccaa ccggatctcg  65322 atgcttgtgg acgggtttaa agtttccgtg ctatttaacg tagaactata ccgactcctc  65382 gcggaactcg ttcatttaag aattcgcaca aaagcagtct cattttcgga gtggctagat  65442 actagggggc tatctgcgga ctgtaaacaa tttattttgg aaaatgcaga acatatttct  65502 gcggtcgtca aagacttcta caacggaaca taccatcagc ttgcaagagt cggtcttcag  65562 tcagctcaaa aatatgaatc gctgtatttg ggaaaacttg gcaatggaaa gctggagagt  65622 atgggtcaat tttttactag actctctgct gaggctgcca gaggagcttt taatattccc  65682 caatttgcac aggcattgga agttgatggc caggttacac cggcagacgt ctttactagg  65742 ttttttacgt gtttgagtag ccaactgatc gtacctccta ccccagtaat gcttttcggg  65802 ggtacgtctc tggctgctta tgccagttgt tttttgatag attcatctgg cagaaataca  65862 agagaggcgt tcaatgtcgt tgccgaagaa gtaattccga tcatgaataa tcgcggagga  65922 attggaatat cactgccgtg ccttggcgct ccccaaaatg agtcggggtg cctgggattt  65982 cttaaggctc tcgattcgct agtagccgcg agcaacggcg ccgcaaagcg gccaacaggg  66042 ctttgtgttt atttcgagcc atggcattgc gacacattaa agattctgaa aattcgtggc  66102 agctcggccg ggaatgaaga gttcagatgc gataacattt ttactgccat ttggatgcct  66162 gatcttttta tgaaaagact gcagcagcca ggaagtaaat gggcactgtt cgaccatagg  66222 ggggagcatc tgtctaactt gtttggagag gagtttgaga aggagtatga acgtcttgaa  66282 agggaaaatg ttggagtggc cactatccct atcaccgaga ttatgtttca gataatcaaa  66342 agtgcagttt ccaccggcac tccatttgtc gtctttaaag acgccttcaa ccgtcactat  66402 ttttacaata tgcaacaccg agcactgaag tgttctaacc tctgtacaga aattgcccac  66462 atggcagact ccgatactgt tggagtatgc aacttgatta gcatcaattt ggcggcaatg  66522 gtcaaaaatg ctcgcgcagg acttgctggg aaacccggca gcttcttcga ctatgaactc  66582 ttaagggaaa cggcaagaac tgcaacaatt ttcgccaatg tcatgatatc acttggaaac  66642 atgccaagcc aacgggcaca gagcgggaac cacaggttac gatccctcgg tgttggagtc  66702 cagggactgc acacagcctg cttaatgcaa ggttttggga tgactagcgt tgagggcttt  66762 gaatttaatg acactgtgtt tgagctactt gccttggaaa caactggaat cagctgtaga  66822 ttgtgtgagc tggggttgcc tccgtttgat aaatatagag aaagctatta tgccttagga  66882 tggctgcata ttgatgggtg gcccaatacg aagctgagat acaaaaatga gtgggatagt  66942 ctaagacata gaatagatca atctggtttg tacaactgcc aaacagttgc tttgatgcca  67002 accgccagtt cttcccaaat tacagaagtc agcgaggggt tccagcccgt attcgggaac  67062 atgtttagta agatctctac tacaggagag gaagtgcgac cccatttggc gctaatggat  67122 gccatcgacg agctgtattc tgaccaggga gaaaaacaag catttctagc aaacctaaaa  67182 aaacatcagt ggtctgtgcg cgctgctctg ggaagtgctt ggtcagagtc tcacgtcctc  67242 gcgaaatttc aaaccgcatt tgaactcgat caggaaaagt tactatactt aagcgcacgc  67302 cgcgctccat tcattgatca ctctcaatca aacactctct acatcaccga agacgtggac  67362 ggtactttaa gtgcgtcacg tgtatcccgc ctactgcaag ttgcctttaa atacgggctc  67422 aagactgcca tgtactactg caaggtgcgc aagatcacaa acaacggagt gtttgtcggg  67482 tcttgcggag acagcctcat ttgctctgcg tgtcaataat cttgctaaca tggcagaata  67542 tcagtccaca gactattatt acttgcccca gtgtgatgac attcgcgagc tccgagacct  67602 gagcatagca aataactgga acgaatttga actatgctac agtcgcgatg agaaagatgt  67662 tgatctacta actccggaag aactagattt ttacaaattc gtgtttgcat ttctggcagc  67722 cgcagacgat ttgataaatt tggatatcgg gaatctcatg actttgttcc ctcacaagga  67782 cattcaacac tattatgctg agcaaataag aatagagacg gtccactcta gaacctacag  67842 cttggtgcaa atgatccttt tcaagggaga ccttcatgcg cgagacagat atgtgacaga  67902 agctattaaa gatcccgcca taaagaagaa gatagactgg ctcaaccgtg tccaagtaga  67962 aacggatctg acacttcctg aaaaatacat tctaatgatt ctccttgagg ggattttttt  68022 cgtggcgtcc tttgccgcga ttgcgtttct taggaggcgg aatatttttg tggtgatgtg  68082 ccagtcgaac gacctcatca gtcgcgatga agcggtgcat acaacagcat catgccgcat  68142 ttacaacaac tggctcgggg atcacaagaa accatcggct aaacgaattc accaactttt  68202 caaagaggcc attgaaattg agtgtaactt tttagcatct agagcgcctc aagcttcgag  68262 actcatagat ctagaagcca tacagagttt cgtgagatat agcggagatc gacttttgac  68322 cgccatcggg gttcccacta tttttgatga accgccccct gatccgtcgt ttccccttac  68382 cctgatgtcg atcgacaaaa atgtcaattt ttttgagcac cggagcaccg cgtataatgg  68442 caacgttatc aatgacttgt aaaagaaagt tcaaacagtc aagcattata taaataaaaa  68502 ctactcagac agatattaac attttatact tgttcttgtt cattttttcc acaaataact  68562 tctcgtaatg acggcagagg ctttagatgc cccttcttaa taagctcaga aaaatatttc  68622 ggggcatcat tagaaaagta tttacacaat acttcatata cagcgatcat gtcgagctgt  68682 tcctgaacca cgggcacgcg cttaagtagt ttgtgtctgc catttatttc tcgtgggccg  68742 atgacactca aaacgtagga cagaaactcg tcattttttt catttctggc gccattattt  68802 accgaaggat cctgagggcg gtaatcaatg tccgagttac catcgtcggg aggttcttga  68862 ggaaactgag tcagtacata tacatagcgt ccaggcaaag gttcacgcac atctgttttt  68922 attccaaaaa catcactatc cgcaggcaag accccgcgct gctcaacaca ctttttggta  68982 tcgttgtttt catttttttc gtggtcacgc cattccgcca acctggcaga aaatgagtat  69042 attgaatcgg tagaatggat gttagtatga cagtccacac atgcattggc aaactcttcc  69102 ccggacatgc ccaaatattc aataatactt ttataaaaga tagcgactgg aaatgctggg  69162 gtcagatccg taatcacatc acaacccatg aatactaagt cactatcgct agacaggacg  69222 tacgccaccg tgcgggtatg gaatagattg gcacatgcat agtcggcttc cattccttgc  69282 acatagaccg tagcaaatcc tgcggtagtt attaagtccc aacataactt gtagtgcgga  69342 ggacgtattt ttcgacgtgt cttgtaaata caatccttgg gctccttaat atcgctgcat  69402 cttcgcgcgg caggtgcagt cggaacagat tcttcctggc taaggtgttt aggttccttg  69462 tattcgcgcc gacgcccacc atcaaaaaca aatacaggat agcacgattt ccgactgagt  69522 aatgaaaata agaacaccaa acttttaaaa gttctctcca tgcatcccat atttccgtcc  69582 gggtcaattc ttcgcaaaat tagaaaaaga acattccatg tgtcgactgc cagtggaaga  69642 taaaatccct tctcaatttt taacgataaa tgaacactga gtccatggtt tcgaataaag  69702 cgacacattc ccaagattcc catatcggta tatttcgagg agagcaaatg aaaagacaga  69762 agcccgcaag tctcgtatca gcaaactaat atttagggcg ctttgaaagg cggtatttgt  69822 atgtacaggg gttgggcaat ccacaccctc gtaatcgcat tacacataag gtgtgacatg  69882 gtggtagccc caaaatttta tgcacaggct acagttgtat ataaaccgcg cggatgttta  69942 ggagatgttc agagacaccg gggaagggaa cgaacgtaca cacgaattac gttcgttgga  70002 agatggattt ggatactgcg acaagaagta aatgtttgct ccgactaact ttaggaaagc  70062 atgggatttc ggtggtttcc cccattgcca aaaacatgcg tgggattctg caacactctg  70122 tgctggcttt tatgaatgac tgcatagtaa tttcctgttc cgctccgttt gggatggctt  70182 tcttaaaaat caagtggcaa ttgttcgact catttgaata tttatcagac gatgagagta  70242 tactacctat catgttcaat aatactatac accagcgccc tggagacctc ttggattttc  70302 tgtgggactc taagaaaaaa actgtcggaa taccaacttt agccacattt actatatcag  70362 aaagtttcaa atccgatgaa actagggctg tactcagact gaaagtctgt cgcgagatag  70422 aagagagcgc gtcgtgcccc caaaatacgg aaataagcac gaaattcaag tactcttcat  70482 cggatggacc tcctatatac ctgcctcgtt ccgataaggc atgtcgggcc tcgctggaac  70542 agtattcgtt caatgaaatt acaaagtggc tttcgaagat ccctaaagat aactcaatta  70602 cagtcacact cacaagagag tatgtcacgt tctcgtccgc agaagacgag caacgcctaa  70662 cctttaacgc gaaatggttc ggcgaccaga caagtattgt atcgtctgcg tcaattttgt  70722 ctcagctatg tggccatgaa cctgcgccta aaaaaagaga gctctcaagc cgagccatcg  70782 gtaagaaact aaaagaagcc cgcattatcg ctgttcatgg ggacaaagat gcctgcccca  70842 ttgtagtatc actctccaga cccggatctc tgaaacagtc actagggtgg ctgaaaagcg  70902 gtccttgggg accaccgtgt cttacatttt acaaagactc tgtgaatagt cttggagtag  70962 aactctctga aagaggagga gaagaattag ctgctgggat ttttttcctt tccgcgtttt  71022 ctgcagatgg agcaatctct gagcaatgcc atgatgactc agacacagcc atgcacgaat  71082 ttttggcgga ggaggagcgc ctcatacaac agaccacgct atcccattcc aactcaagta  71142 agaagaggtc gctcgaaaat tacgaggaca ccgatattag cccatcccac cacccacaaa  71202 agcgggggaa attaaagaac ggctcacttg cacggaagaa ctaaaatcgt ctcctcgcgt  71262 ctaccgggga gcttagttta ctgccagcgt ttgtgaccca agtgtgcacc gtgatttcaa  71322 ctctaccgca actatgacgg gggctaccat aattgatcca ttcgcaccgc ccaagggtaa  71382 atggtggccg ttcaatttga acgggatagt tttttccttg atgatgttta ttatattttt  71442 agcctggata ctgtgcattg actatggact cgcgttagct tacattacct gggcaaagct  71502 ttctacgaaa gaggcaagat tcggatggat gatcggacta ttggtggcta cgattactgc  71562 cagttttctg gatattcaat actcggctca caaaacagtc cgaatttatt ttctggtgat  71622 gctttctatg gcgagcgcag taataattat atttctcatc cattccaaca gccccaatgc  71682 cgcgatagtt atggggctat tctccgtttt ttcggaagtt tgcttgatac tgattttggg  71742 atttcaactc cgaccggcca ttttctgcag cataaacatg acctggctct ttcttgaagc  71802 catgctccta aatttgaccg tactttcttg gaacttgatg caccttcgag taaaccctag  71862 atacttggaa ccgttggccc tttttactat taacattttg gcatacaatc cctctcgttt  71922 tttgctcaag agtgattttt ttaagaccag catgataact ctgacgggca gtatagaacc  71982 attttccgaa gataacacga tttatacacc cccaagacaa cataaagata ctcgcccttc  72042 actgaatgac ggaccaactc ggtggtgcgg ttactgtatt ctcgtatcta caacattggt  72102 tactgccgct ttcgcctgca cattatcatt accgttcctg ggcaaagatt taggtactgt  72162 acgcatcggc atgcaaacga attttaaaat cctcatggta gcgtgcggtt cggttttggc  72222 atttggatct acttgcattg gaaaactatg caaaatccat atcgttgtat ggttcgtgat  72282 aagcatacta ttaaccttcg tgtctctgct atcactgatt aagttgattg aggacccagc  72342 tggcattcca tttggtgtca ttcttgcatc ggtttcttgt ctgtttcaag ttggagccct  72402 ctttttccga gaattaaaaa cggccaccca tacacaagga tggatttcat gcgcccttct  72462 tttctgctcc cttttcattc caattgccgc gccgcttgtg tgtgagtaca agctctgaat  72522 tcttgtctaa gggagacgtg ccaattctga caacgcccta agccaacaca aatgccttcc  72582 tcaatttacg cgctagcttg aacattccaa caagatgaat gcatcgctaa catggcttgc  72642 tttaactttt aaagttacct tgagtttcag cctgctctga atgttttcct ccaaacctaa  72702 gatgttctta gttgtacgat ttttgtattg cgaataccac atcatccagt accaaagtac  72762 taatggtcgg caatcgaata gaaatcaaac atggcacgca ggagactgtc gcctcgaaac  72822 tgccttcgcc gagctgcaga aattcggaac gaggacatta gaaacagatc tacattccac  72882 cgagcaaccg tttactggag aaaatgctag agaataacct aacaggcggc cttcaacttt  72942 cactacttaa caactggaac tatattgtcg aggattgtca tagtcacgaa catgatgcag  73002 tattgcttcg gatgcaatct atattttgac attgttaata aagcacatgt actatgcaaa  73062 gtttgcattc gtgtaattcg tcgagagaga aagttacaag ttcgattctc tcgcgctagg  73122 agtgtttcca cgtgcgaaaa cgcaaaaatt ttcatattat tcgggcggac tgtgtccata  73182 gtagctaaat taccgcgatc tggagactag ggcattcacg actcaacatg cagcatcaga  73242 gtactgcgct agtttcgagt atacttttgc tcttgagcct gcaaagcctt gcgtttgaat  73302 ttttctgtga tccgccacac gtttttcgag ggcagctcgg tgaccccatt ctattgcaat  73362 gcttcagcga cagacctcta acccacgaag aatctgtaaa agtagaagta attcgacacc  73422 cagccagctt agttgaaact gcgctaagcg cctacgggat ccccccttcg ctagatccat  73482 ggagagctac tccaagaact ctctacacat atgatgccgc tactgattca atcaaggacc  73542 taggatacat tggtgaagat ggaattaacc caccatattt ggacgactgt cgttcaggtt  73602 ttttcaatgt ctctatcaag tctagcatga gatctcacat ggcgcgttat cagtggaccg  73662 caagtcgagg gtctacaaaa ctaaatagct cttttatcga cgtctttttg gcaagaccac  73722 ctacaactgt ccgcatcaaa tcagaagaac tgtacgaaga ctcagataag gcttcgcact  73782 taagtgttga agcgcttggc gcttatcctc catctgctgc gctgggtaca tggatgatac  73842 ataatgcatc tcttgctgaa aaatacagtt tagaaagaag agttctttat gcatcaggag  73902 agaatggatc ggtggatcag acatgggaac tggaaatacg tggagaagcc agccagcccc  73962 tcccttccaa aattcaattt gtatatcgat ggacccctcc tgaggacttt gaaatgctac  74022 gacctgaaac tcgcttgtta aggttgactc ccagctggat tagcaagccc cgcatcacgg  74082 tacaattcgt ccctcctgcc tatgccctgt gtagagcagc taatattata gacggccgag  74142 gatttattga atggatcgta gataatagaa tttcgacgag cccacaccag acctttgttt  74202 tggatgagcc cgaggggaaa aatatcgtta cactaatgga cgtcataaaa ctaccaccgg  74262 aggatacatt tcaatctgcc tctaattacg tgtgcgtcat aagaggctat gaacatgcat  74322 acagatatct caacgcctcc ttaatgatag ataatctgcc aatgcggcaa ggattccccg  74382 cagtcgctgc gatttttatt ataattagta tcgcttttgt gggtgggtta ctagttgctt  74442 gcttgggcgc atggtgctgg aagacaacat aaacgctcat ttaataaatg acattacaaa  74502 cgtgcactac tgtctgtcca atttatttca tcggaaacct tgcactggca ggtagtctca  74562 agcaagccta aacgatatta tagatttcaa tatttctacc agatccgtcc gatattccca  74622 aatgattgga gaaatttctt ccttagtcat cttccacaga tcttcgattt tgcgcccaag  74682 ctcaaaattt ccagtaattt cagaaacctc actggccgcg tatgcctcct cgtacaagta  74742 aatgcagacg agagccgcgg cggttcgctt tgcagtgttg accaaaattt cttgtggaat  74802 tttagttcga aggcacgctg cggtacttac atctttcatt aaacgggttt ttgccgcatc  74862 gaggagtacc gctgtttctg ggattaacgg gatactctct ggtggatacg aacatctcgg  74922 tttgggttgc actttgggat tcaaacaaca gctgactagt gcatcttgca aaacatagtg  74982 cgtggccagc aaagttttca atatattgag aacaacttcc gacaattctt gtaattgtag  75042 agtttcgggg ctctggattt ttttgagccc gcttttttca atatatcttt cacagacaac  75102 tgtaatttcg tcaaagtgac aaggatagtg cccgagcata agtaaataat taaacagagt  75162 ataaggaatc actgagcttc gcccgaatga tgctttgctg aggcatcgtt ggccagggta  75222 tggcagcgtg accagaatgt cagcctcgtc gcgcaatgta cttttcagtt ttgctccatc  75282 agtctcccat gaaagtccga tagttgcgag aagtccatgt cgtgaaatcg tggccgcatc  75342 cacgatcatt aacatcttcc agagtcgctt taatactttc caacattccc agaaattttc  75402 tacatcttcg tcgaacattt ggtcggggta aatgtgtttc acctgcacat gatctgagag  75462 gatagttttt ttcttacacc cacgcagggc ttttttctca tttcctactg cgaccgtact  75522 aatgatttta cctcccgtaa taataatagg agtaaaaatc tgtgattgtt catgaatcgg  75582 ttctcttgtt agggagttca agaataaatc ttctaaatct tcttctatga acttcggcag  75642 agtagtaata tgtgcctgcg ttagcaccag cgcactacta tctacttgat acctaaatcc  75702 tgtcgtcgta ttgattagat ccggggttct aaataacctc agtactttat tcctcagaga  75762 gacttcccca actcgtattt ctagcgtttc ggaaacctgc aggccgacaa atattttgca  75822 caagatcggt cgtctgatct ccacggtaaa attatcaatc aattcatcat gtggagattc  75882 tgaaatttca ccaagatgag aagtaaaaat gtattcttcc tcctggcggt caaaaggcat  75942 cccattcaaa cgattcagtt cagtgttaat aatcgcacac atggtagggt gtttgatcct  76002 aaaaccgaat ttggtaatat tttttatcgg cccgcgagta gagatcgagt agatgcatcc  76062 tccatagata atgtagagtc gtccgctgtg gtgatctatg tagcgtgcag gagactttga  76122 tccgcgcttg gagacttctt gaaaagccac ttccatttca gtccttctcc aaagagcttg  76182 cgcgtcaagt gacggtaccg ctattggtgc tttaaaagta tccgcccttt atactgctcc  76242 aattctatcg tagcataaag ttttgacgat catacagcgc ctgcgtcttg gcaccaccta  76302 caaccaaacc tgagctctcc ttcaacatga caactggaaa cgaagccaat tcaatccgcc  76362 tctcttcagt acaagtaccg ggaaattgtg agattgacaa gctgttggct ggatttgact  76422 acgaaaaaga acgtgatggt gactattcga cactgaacga aggatccata tttctaaacg  76482 atcgccttat agcttcggga agtgaagata catctgatct acaatatacg cgaatgagga  76542 aacaaacttt ttatcctagt ttagcccttc ttttaaaaat actctgttgt ctgcctttct  76602 tttggtacgg gaattgcacg cgagagaaat atttatttgt aaacgctctt attatcactg  76662 ccctctcctg tctagaagga ctattgattt ccttttttgt gtaccgaaac gtgaaggctg  76722 atcgtcttcc tttaaaaggt cctgaaaaac tcattcaaat ggtgttatgt atgataacgg  76782 ctatatatgg ggcgattatc ttttcaaggc acctttttgc ggacgatgac cttgctattt  76842 caatatttgc gaaaaactgg acagacacac aggaagcctt gagaattggt cattgttatc  76902 tctctccgta tttttcgatg tgggctgcct gtctgtactt tataattctt ctatacgacg  76962 ttattgacgt gacacttcct cttttgtggg cctggacaat attaaggaca gcaataagct  77022 tctaaatcgt tttactgcta cacgaaatcg caaagttagg tggaaggatc gcgttactgg  77082 cctcataaac cccgccttga agcctcaccc ccggtaaaga cacgataaag gcagagactg  77142 cagtattata acgggctgac ttcgtaggat aaaggcaacc tacaactctg tgtgctcaat  77202 atttagccat gaatcctgac aacgggatcc cgcataacag tcatcatgat cgcgcagcat  77262 tcccaagatc tgctgcccct ttcgtagcat ctggggaact gttaggaatt cttcgagaaa  77322 attgccatgc gcatctatat gaatggataa gccgcgaagg ggattgttgc tacagacaca  77382 gctttgatat tctgctggga tcttatttca atacactaac gctcaccaac tttctagaaa  77442 ccggactttc agttgcatgt atttgcgtga aatttccaga gttacgctat gcagaccgag  77502 gaataatcca gtttgtagtg gctaatccca tgattgcaag aagtgattgt gaagtacctt  77562 ctcggccatc atttacctac atcagtaaga gatggtctag gacgacatta tcctcatccc  77622 ttgtgatttg tgcaccagct ctgggcttgc taagtggcga gtcacttgac gggaccgaaa  77682 tatctgagtt ttctagatta caggcgttaa accaacttgc acgaaacctc aaactaactc  77742 tagactcatt tgaaagagga acaataaatc atgtgctgag aattctaatc cgaaaagctc  77802 caccacttcc tcttctacga cccatgatgg cggcgttgga atgtgaacga gaaatgtcta  77862 cggttgccag agcaaatatt atctccagta tgaaggcagc cctatgtgaa gatttgtttt  77922 tcatagataa ggagaggggg cacgagactc cggatttcgc ccgaaagcta ttagccttaa  77982 ttaattgcac gcttcctagt gtaaccgatg ctcgtgttac acacataggt ccggatggtc  78042 gacttataga aggggtgatt gttacaaccg aagccgtaaa aggcctcatc gcggcccgcc  78102 ttggcatcga aactgcccgt gccaacgtcc cggcaatgta cagtgagatg gtattatctg  78162 gaaatagcct tgtaacggcc ttactgctgg ggaaaaccat tcgtaacttt gatgaagccg  78222 ccgcaaattt gttaagcttt ttggacggtg aaaaaaatct cgccgatttt ccagaaatac  78282 cgtctaataa cgcagaccaa gttccaacaa tgtccgtcaa aatgtccctg ctcaacgtgg  78342 gggaccatct tgtcagcata gaagcactag agcgtgttta cacgcgcact ggagttccct  78402 acccactctg tgaaaatgtg gatctgacat tctttttccc acttgggttg ttcaaacctg  78462 caatagatcg ttattccaca tctgagattg ctcctgcggt cggagccccg gattatcgcc  78522 agtttccccc tacagaaatg tactttttca ataaggacgg catgatggtt aaactcacgt  78582 ttgaagactc attgccaacc gtcgcacatc ccatagcaca tggaatgctg gaagctttgg  78642 cagaactttg tcaagaacca tgggtttcaa atcgcagacc tgctccgatg gggttcacaa  78702 ttcaacgtat cggactaaac ccacctcgcc tgttaatgat cgaattcttg gaagcggtag  78762 cacgtactgc ccctgcacca tacccagacg caactttaat caatcggaaa aatccagatc  78822 aattctctag ccataccaac ccgttccttc cattagaggt tcacccgttc tatgatgtgt  78882 acagagttgc gcaggatcta actattccct gtgatgaacc attgtttttc ccagccgaac  78942 caataacact ggccgcctct aggcgactct gcaacgggga tattccgttg ccgctgtctt  79002 cagtcgattt tagacttgcc agggggtact gtatcgctgc aggaagacac agactgcacc  79062 cttcaacgga cgcagccata gaaacaacac tatctgatgt gaattaccca ctggctttct  79122 atgtaattga agcttgcatc catggcgatg aattgatttt catggaatcg cagaggctag  79182 tagcgcagtg catcaatagc tattggcata catcaagggg actagctttt ataaacagtt  79242 atccaatggt aacgtacatt tatcacaaca tgactggaat gatcgatcga gattgccact  79302 ccagatatgc ggatgtaatg ggcctgcttc acgcactgag ggaaacaatc agaaattata  79362 ctctccctgc ggaaccaatt ctcctgcgtt ctcatgagga attaaacaat ctcatgacgg  79422 acccggcact ttttccgccc atgatttatg actgtgattc catcctaagg gcacgcactg  79482 catgcgcgac tagaggggtt acgatctcca ccttcggaga gagagcacct accgtttctg  79542 tcagggaata tccagcccaa gtggatttca ccgttcttag taacactcta aatcacggcc  79602 cagcatatac tgctggcgga agacatgaag gaggaaccca tcatgactcc gaatggacag  79662 tactcagcaa attgttttac tatgtttttc tacctgctgt ctctagaggg aggtgttgca  79722 gtgcaggagt cgagtttgaa atgatctacg atctcattaa cactaccaga cttccagata  79782 cagttgatga aatggacaat caggcaggtg ccgtcgacag aggtcctttg gcggatgaaa  79842 atcttgcccc cgaatccttt aacactttgt tggcaaatgg atccataaat ctggtggata  79902 atgaagctct ggtagcattt atcgctgccg cacggcgccg tcaggcagta cacacgatac  79962 ctctgagagt taactattta cctgacccgg gcttcgaaac tatagacagc ccgaggaatt  80022 ttttggtaga cggagtctta tacaatggaa taatcatgat gaactatgct caatatgacg  80082 cgacagctat tccatctcgc tatttctatg cattgcctgt gaatggattc tttatgaacc  80142 gtactatcat agaagcatct cacagggcta acgtcaacct tactaatgta cctgaggact  80202 tgccactagt tcctacattt ttggggagcg aagtgtatcg ctccataaga gcaccgtcgt  80262 acctatttgc ttcgagagct tcaaactatg cctcaaactc ggtcgcggca tacagcttgc  80322 tggcaggata cttcaagacg tctcccgtcg cacttgtcca tcagttgaaa ttaaatcttc  80382 atccagggtt tgctctcact gtcgcccgcc aagacagatt tgcggcggat caaattttat  80442 ttgcccgaag agcatcggaa tcttactatc taggatctcc agttgtaacc aaccgcccag  80502 agaatgattc tctcgtaatt gagataagtc agccaagggg gcacatagat atgggtctgg  80562 gatttacagc gagtcgtgtt cctgcaaaaa ttaatacagt ggttacagac atgggcaatc  80622 attgccaaaa tctcttcaat gcccgctacc ctggtcagtt tcgccatgcg gaagttgcgg  80682 attttattgc ctctgaaata acagacaatg actctacagc cttgcctaga gcgcagcctc  80742 ccattctgct gtcttacgaa aaggcaccca tccccccttg catagagcgg ggacagcttg  80802 ctacatgtga gtttctttta actccagtga ctgccgatct tgcatatttt tatacatcag  80862 caaatccccg agggcgcagc agctgtatcg cgtgcaccaa ctgcgaggat ccatgcgcga  80922 gtgaaactga aaaggctatg tatgaccatt ctaccccaga tgcagcacac ccgtctaggg  80982 ctacgaataa tgcttgggca tcgcaaaagt attcagtggg agacaaaatg tataatgcgc  81042 ggcggggttt tattacggcc agtgattttt acagtcctct gagcaagttt atgacgccat  81102 ctagagctga agataagagt cggtgcctgg ctcgtttgat gagagattcc tctgcggcaa  81162 ttagttcagt tacgggtgac actgagtatc aatttgtcgc cccgcccggt gccaacgaat  81222 tagtaactga tccgtgcgca atgtttcaag aagcgttccc acccctttgt agcagtgaca  81282 aggtcctctt cgcaacctac gaaggcccta atagagcatc tggatctggt gcaagagaaa  81342 accactttgc gcagtatttg attcacgata agtctccaat cgccaatgcg ttaaaaaccc  81402 catgcaacac gcgtcgctaa gacggaattc gcaaacgatt tctacccgtg aaatcacgtc  81462 accgcctggt ctgtttgggc ggagcccttt caaaacttct tcataagtgt ttcctgtgca  81522 gatgatttgc ccacggaggg atatatagcg catacctaac taaatttcag aggacagaag  81582 tacagatcgt atcagccaga acgatgacaa gcggacagta cgagatccgc gttgttctgc  81642 caaatggact gacacgagat gaagaagatc gtctcagaag cttgcgtggg actattttaa  81702 tggcgccaat tttgcgaagg tgtgtttttc tgcacgaaat cgatcaaaaa tcatttttcg  81762 cccatggaaa agaaccagac tatgcaactc tgctcacagc ctacaggaga cgctttccca  81822 tattaatagt ttgtgtcgaa aaccgagaat tgagcgccat cgcgctatcc ataggatacc  81882 cgcgcggaat cagcgtgcgc aacactggtc catttagttt gaacaacggt gatctcgtgt  81942 ctttactgcc acccattacg aatacgcgtt ttcgcgtcga cttgccatcc tgcggctctg  82002 taatcgagcc agccatgaca attcctttcg agatcggaac agaactgatg ggaaagattt  82062 ttgcagggat ggcctatgac ttctgcgtta gaaatcaaat agcgacaacc cgtcctcgtg  82122 atatttatgt tgtaacatat aagaacaaga cactcgatct atctacattg cccccgtctg  82182 atgcggctgc acttcaagat acaatgaaaa gcctattttc atcggttcta ttttcgatac  82242 acgagggggt catgtccgtt ttgtcactaa tgcctgcttt attggcagga ggggcgaacg  82302 atccatttct caatgccatt ctacaaatgc aaagcatgac cagactttcc gtgcagcttt  82362 ttaatccacc cgcgctagaa ttaccggaac ctgctggcag ctctgggcga tatcatgtat  82422 ttgatgcttt tgccgcgtgg ctttcaatgt ctcaccgact tggtgattta ttcaatttga  82482 aaccggtcct caaagtagta atgttttact cggatgattc taccgcagac gagggagacc  82542 tcctcaacgc cattgtacct taacccgtct gtgcatctct atgaaatgac gagacatcat  82602 atctctttga tttgtgaata aagtatttat tatcgaaatt gaatttatgc tggtctctgt  82662 cttctaccga tcggtgcgat ggggtgatat ccggcagtcg gggaaccttc tagtgctaag  82722 tatgtagaca ttacagcggc cactaacatg tcatcgggta cgcggccctg cttaccgcta  82782 aaagttcgaa aggaatcggg cccttggcca tgagtaacag taatgttcct tatttggtct  82842 acgagatact cgcatggatc tttactgagt acaagggtgt tcgagacaag ttcctgggaa  82902 gccataaagc gcccagagtt aaataggctg acaaacaagt caaatgctct gctcttttgt  82962 ttgtttagaa gataaaaagg gtgagccatt gcagttccag gctgccgact gtgggcaaaa  83022 gtcagagaaa agcctaacct cctacgcaaa gaatccaaaa gctctgtcaa gttgccggct  83082 atggcagcag cagaatcctg actcgaattc ccctccaccg ctatacgaat ttctcgaaat  83142 gcgccagcgt gaataagaca acagtatgct atacacaaat acacgcactg ggcaatttcc  83202 agcgccgcct ctccggtcag tgcatccaag taaaaatgtt cagctcccat gagaaccata  83262 gagtctcgca gcgcagtcac taatgctatg ccagtaccag atgcgcttct gtttgtggta  83322 aatgcaggat ctacatacac agtgagaatt ttcccgagcc cagagatgtt gggtttactt  83382 gaggtagatg gtctgtaaat caaaaactga tccaaagctc gcacaggaac aagtgtacga  83442 tcggagtcat attttccagc acgtccaccg gcaatctcat gcataaacga atcttttacg  83502 aacaaatctg cggtatttcg aacctcgctg tccatactaa caaagactgg tttgtgcaaa  83562 acataacaag aacaggtagt cacgtctgtt cttttctgga tttcaggcat gtgttcgtcg  83622 catatatagg taacaacatt aagcagagag tttgtctttc ccttaagatt gtacagtaga  83682 ctcgtgttac tatggcaagt gttggtagaa gatacgaaga aaagtttgca gttagtctga  83742 ttcaaaaacc ccatcaccgt gtggagagca gccggtttta taaaatttgc ttcatcgacg  83802 aacaggaaat tgaagtcctg ccccctgaga ccctgcaaaa acagaccagc aataaaagat  83862 cacactcaac ggacatgatt cattcaccag cgcgtcgcaa tggaagtaca tgtcttagca  83922 gcgacatcga agctattatc ttgcggtgaa caggagggag gtcctgcgcc gatactaagt  83982 cacctagtcc tgtccacgca gtgcttagaa ggatttgaag ttccggttca ccttcttagt  84042 gacaataagt tttatacaga aattcaaatt cgacaccacg gatgttttga ctgcacagag  84102 tggaaacaag ttttctcaac ttttgtcgga caccgggcac tcgacaaaat tctattacct  84162 gagttgggga attgtcaaga gcgccccttt cgaattacgt atgatggtgg gaatgactgg  84222 ggaggactgt tcatcactat cccggtatat tgcgacgcag aaaagatgac atatgatgat  84282 tttactgcgg tggctatacg aatagctata ggagctgcgt tggaagaata ttatgaactt  84342 ctattcacgt atggagagct ggtaaactct tctactcgct acaatgtaga cagtgccaga  84402 ttagaggcgc tttcgtgcca gctattagag tactctccat cacatttaaa tgaagctcaa  84462 accaagtatt ttcgcgatat taaaaaacgg ctctccgaat tactcggcaa aaaccggcaa  84522 tatgtaataa gcgcagctga atatctctca aacaatctac aacgatttga tgcgccaccg  84582 gacacgtcag ccaaacaggc tactataaaa gaaaggatag acgagtctac acatctcttg  84642 aaacaggtgg caggcgcttc catgctgccc atgaagaagt acacccccgt tccccaagga  84702 agtgaaaatt tgagggcggt ggcccaaggg ttgagcgctc tcgtaaaaac gctagggacc  84762 cgaggcactg aaaagatagc agatgctcgg ttttcatcaa atactgcaac atctgaatta  84822 gagccaccag gttgcagcag aggtttcgaa aaggcggcac caccaatgcc tgatataaga  84882 aatgttatca tgacagacag gcaaaaaacc acaatgtcta ttacatcaag atcggtaact  84942 tattatacta tgtatgattg tctggcagca gcctgtgaaa tcatggaggc agaaaataat  85002 tctaccagaa gatcatggtc actcggaaaa atctcaattg tgttgatgtc gtgctataac  85062 tctggagctc caatcatggt tgtaaattat tcccaagact cgttaaagtt atgctatcca  85122 aaggttagat ctggagcttc aatactagct acaataatga gaccgctggg agaaacctca  85182 gtacaaaatt taaccgaagc agctcagcaa gaaatagtga agaatgcccc atgttttcaa  85242 tgtcctttga aagctttgaa agatccagat tctctaacta aattgtttgc agtggactgt  85302 gaacagttgc tcctttatag tttccaagcg cgcgtggctg cagcattcac cgcagcggtg  85362 tccgaggcca ttgtacgagc aactcacaca gagagcaata ctactcggct cggacagcta  85422 ataaactacg acatcccttt ataccagcca tttgacagct tggaagcaaa ctggccaaca  85482 caaatagcac gtatggcttc agacctaatg gcagttctct gtataactct tcaaagtgaa  85542 cgcttgtccg tctttatgaa aagtggaata tggcgcgcca tcttggcaat gttaattcga  85602 aaggaaaatc accgaagacc atacctggcc cctgttcatg tggaggatga cgtgtaccta  85662 ttcgattatt tcagattcgg atcgagtaat attgtaagaa ttaccacaga gcctgttgtg  85722 ttaaaggctc gcaaacccaa aatagacggc ctctacgaac ttgatttcat cgctagtccc  85782 ccctctggcc tccacccgtg gactaaacaa aaattccgcc caggagaatt tcactcctat  85842 atatgcgttg gtttcaattc ggccctgaat gctcttctca tctttcctgg agggtttgga  85902 ttagaatttg attttggcga agcactaaaa gaagtctggg aggatcacct ggatcactta  85962 gtattgaaac gctttagtag gcgtgcacca tactaccctg aatcttatct cccgagctgc  86022 gaattcatgc aaaagataaa cacagctggg tagctagaaa ttgcgccatt ggcacgtctg  86082 caggcattct tgcttaaatg taatagcgta ggacatagaa agcagttaat accgagtaat  86142 taacaattag tggtcaaatg gattactgtt aggactaggt aaatgtacag aatacttaca  86202 ttcgtatttt gactggaggc gaagacaatc gcacttttgt ttccattgcg aaatgtaaat  86262 gtgatcgttt cccccttgac ctgttctacc tgttttgcgc caaaccattt gtacaggcga  86322 gtaaaaattt caataaaaac gggctctgtt gcttttctta agtgggcagt gtatccaatg  86382 cgtattcctt caaaagatga aaccagtagg gaaatgagcg gaactaaaaa ccacgttttc  86442 ccgtgcctcc ttggaactaa aaatacagta gttctttgtc taaaatgttg aatgatattc  86502 tctgaaaata atgaggtatt aaaaacggta gacaagtatc tattacttcg ttccgtggat  86562 ccttccccca agcaaatact tgacgcaaag tatgtagcat gcattaagat cattttttga  86622 aataattcca gtgttccgtc tctctgttcg ctagttgttt tttccaactt tgcccgtttg  86682 tagtcatttg aagcttgacc agaaaatgac ttttttaaca gtgtgtgaaa tctgtaaata  86742 aagttgttca gttgggaaaa ttcatcagac ttgacaaacc cccggtagcc attcattatt  86802 ccgtcgaatg ctttgtcatg tattagggag tcgcttggag caaacctggc aaaatttaag  86862 tttgcgagtt tctgtcttag tgccggaagg atactgtcct cggctgcacc tgtgaaaagt  86922 tgccgtttag gggatacctg taaatcgttt agtgcggcat cggtatctgt gtacttggta  86982 gaataggagg ccagattttc gtaaatgatt tctgacgcca tatatgaaaa cgcacgcgaa  87042 atagcacagc aattatgaaa agatcctata ggggcaatgc acgcttgatg gcgctgtgga  87102 acatcaatcg caaattgaaa tctgctcgcc ggtgaattca tggtatccct cgctgaatca  87162 ttcctatcac atatacttgc agtagttata aaattggagt cactcatttc ttgcccatcc  87222 acgtcgtctg gtcctcgttc cattgttcgc ttacgcaaag catcgcggta tcttttgaca  87282 atttcgaccg attcctttcc aagcatgtct tctgcgagag actaccgaaa cagggctcgg  87342 gccgaagtta tgaactacat taaagggcaa gcctataaag ctgcggtcat cgagatgatg  87402 tctctcaaag ttcctcgaat gcacccagct ctgcgctatt ttcttgcatc agctagggag  87462 caggaagctg tttcagagat aaacgtgcgc tcaaataagc gcctcagctc tgtcaggtgt  87522 catgttgctc ggataaaagc agctacagag agtcagagag cattaagact ggagttggat  87582 ggctacagaa gatatctaag gaacgacttt ttagaaacat tcgctcaaga gtcagaagcc  87642 attgcagatg cggagctaga cttgcagcgg gcagaagaag aaattgctct gtatatttca  87702 ggtgaccctg atcgcagaga cagcttagat tgtgaggaag acgacctttt attaaaatgg  87762 caactagaaa acgtaaatcc tccgacgcta ctgccatcac cacatgcacc aagtcccgtc  87822 agtcgcggga actcgccaag aactccaccg actcccagtc caacttttca agaagacgaa  87882 tgcccagtag aagaatacta agcaacgatg atggaatcga ccttgagcat atagccccgg  87942 tctcagaaga tttgacaaaa gaatcgagac agcttgcgat ctacataatg ccgaaaccta  88002 acaaaatatg tctcaaggtc aatccaaaaa tgagctaccg atggtttaac ctagaagggt  88062 caacggtaat tggaaatggt ggatatggat cagtgcagta tgccccaaag taccacatgg  88122 cagtaaaaat atttgaatcg gatggtcatt ttcgatggga actagcaatg tccttgattc  88182 tttccaatgc ggcaagaaga ccagaacttt ccgacatcgc caaacatttc ttacagatct  88242 atgccttttc aaaaatcgag agagcttttg taatggaacc gttgagccat gacctaaaaa  88302 cgtacgccaa aagatacaaa gacaatttta caatggaaac attgaatacc ctgacatctg  88362 agttcaaagg acttgcgaag gccttggcat ttctgaacat tgattgcgga ttggtacaca  88422 tggacgtcaa gagtaacaac atactggtca aatgtgatgc gaatggaaaa cttagtcgac  88482 ttgtattggc agactttagt ttgacaggcc ctaatactaa ttctattctc aaccagagca  88542 tgatggtgtg tccttcaaga ggagtagtgg aagggctaaa aattatagat tcaacgactg  88602 taaagaatca cattccatct gactctttta tcatatataa cggccactgc cgagctccag  88662 aagtgataat aaattattgt aatgggaaaa ggtatcgcga ccagccaatg gacgccctgg  88722 aaacacttgg attggatcta ttttcccttg ggcaagtagt gcaggaaatc ttatttgagg  88782 gaattctgtg cagatcgcgc gagttttcgt ttaaaccaaa accaaacact attcatgaaa  88842 aattaagtca cgattacatg atgagggttt tggcatatcg aattgtgctt tccgacaatc  88902 taatctctag aggctgtgat ctaagtttca ccggtccact aagtggaaca gtagaatcag  88962 tgaacgcatc cctatttcga gaattagaca gaatcctttt ccagagtcac gtcgaaatgt  89022 atgagcgcat cgatctacga gaaaaactgc tgaacgtcat cattcctccg gaaaccagag  89082 gactttgtac cctggcggga ctgctatgtc actgggatgc ggatttgcgc cgctctgcag  89142 tgaccttctt ttagagattg tgatcgttac ttataccaat aaactttttc agagctttgt  89202 attacgtcgt ttttttgtga ccggggactc tatcaagggg cgtggttgcg cgataatggg  89262 gtatattttg gcctaatggg taacaggcag caaggtgttc cgttcactag tcttcatctc  89322 gcagaggaga aacatgacaa gtgttcaaga ccagtctcct ctgaacgaaa acatgcaatg  89382 ccagaagact ttagaattgt cggttggaat gcagattccg aaagaactac caggagtggc  89442 aatgtttaca ttctacgatt acttagccga cctggcacgc ggaggacacg cgcaaagtga  89502 cattcttcaa caggaaccac tacatcaccg cctggcttac atcagccacc tgttttcgtg  89562 gctcgaaaaa gaaggtttcg cgtatggcat tttgaacaaa ttttttggtt ctgagcgcgc  89622 tctgagcgca tcagaaatta gtagtaattt atcgcaacaa cagatcaatg aaatcttatt  89682 tttcatagaa tctgaaacga aacaacaagc gtcgtgtgac ctgtggaaag ttttaagaca  89742 atttttattg acggcttcaa ctttgaagtg gatgaaaaat aaaccgtgct caaagccgga  89802 atggtttaag gtacaggaat tcaaaggcgg acaccttggc tatgccacac agtccatgcc  89862 tttgattttt ggcaatacta acgaaagctg cgcccgatcc cttcttttgg gatatgtaac  89922 tggagaggca tggaaaactt cagaagatcg agaagagttt tacaaatttg acgacggcca  89982 tccaccagaa gaagcattta catgtggact tcttttagat aaacggagtg gaatgttggg  90042 agcatcaatg gatatggcga tagtaaagcg caggaaacag tgcgctagaa aagtggagat  90102 atacgaaatt aagtgcagag caaagtatgt gttctcggtc gagaaccaga cgcatccact  90162 ttctcaactg tacgataaaa tgttgcaaca cccatgtgaa aattctattc gcgatttctt  90222 gctgggaatt tcctcaccgg gggtagaatt tgttgaagag agcggaatcc ctacagcatc  90282 agaagctcta ctaacctgcg acaaaacttg gaaaactgac aggtggaaga aaaatttacg  90342 agaacgtgct tgcctaatgg aaaagcgtca cttgtccctg aatagaacca acaactcatc  90402 tgtgtttttg tttgaaagcc cctgtctgga aacaaacacg atcagacctg tccagtggcc  90462 ggacggtgaa aacaatatcg agttaccaat cttcattaac ccaaaacatc agaatttcaa  90522 acagattttt gtacagacat acgtcttggc cgaatatttt gaaacaattc ctatatctcc  90582 atttttagtt acatttatag gtcgcaacag aaagacggta gagcggggac gtgtatttaa  90642 gctcgaacac acattagacg gtatagaaga accggtggaa ttgaactgca agcacgcgat  90702 accggtacta ctaataataa cccccacaac catcgacaga aatcatttca gtgatttgga  90762 ctcgcttggc agagaagcgt tcgagttttc tgtgaaggaa acatgggcca aggtctctgt  90822 ggacacctcg gagaatgttg ctgcccctgc tgttcaaagt cctgcggggg tgacacaaga  90882 tcccgaaaat ctggagagcg cttgtcccta ttagacgaca gctttgacga attcaccata  90942 accgaagaca tgcctatgat tcaaggagga aaaaagaaag agaagcgtag caaaaaagaa  91002 gccgaaccaa ttgtagttga gccagtggct gctagaaggc attgaaacat gttatttcaa  91062 ataaacagaa ctacaaccaa atacatgcgt caacctttat ttgcgttatg cgtaatctaa  91122 attacagttc gtcgtcatag ggtacagttt cataaacgtg cttgtctctt cggcttttgt  91182 atttttcgcg gcgcgttttg cgtttcacgg ttttatctaa agcttctgtg gcaacggtcg  91242 ttaccttgcg gatgaaatcc gtctgtctgt actggtgata tctgtatgcc cgtacgactc  91302 gcagaatagc caaaactaaa acaaggaggg caaatactcc tagaattcct gccgcagccg  91362 gcttccagtt gggatgagtt ccgctatcca acgtattagc atagtgcagg gatgccgtcc  91422 caagcattcc aagagcaatg atgagtccga aatgcggacc aataagtaca tgcacatact  91482 tcgctaaaac cagctcggtc acaatcaagt atatgatacc tatgataaga aatgcaatca  91542 ttgccccaag tgtagaagca gggacactga ctccaaaatt cacggtcatg acgagtgctc  91602 cggacaaggc cattataagc ccccaaactc ccatgataga ggcgtagacg tttaatacca  91662 ctgccttaaa cggtgataca acacaatgaa cttcagggca tgcacttttg agctgtgagt  91722 taaaaactga gtactggtca ggtgtctttc cggaagacgc gaacgtaccc aaaaatacga  91782 tgccggatac aaaaataatg caaaatgtga cagctgatag cgtaagctgt ctgtacgaaa  91842 ggagtagtac aaacaactgc cacgcccata ctgtgataat aatagacatt aaggcaggtg  91902 tgctgatcac ggccgaagtc aattcggacg ctccgtatga acgatcgtga ttacaagtct  91962 tggctctaac tgcgccaaga gtaacgtata tcgccaagag tatccacacc gccatagtcg  92022 cgtaaacggt aaatatcatt cctggcgacg aaataaaaaa aagtccatct tcgttcgccc  92082 ttagcagtcc aggttgattg atcaaaggcg ttgctggctt aaggtgtcca ccggcgacgt  92142 tgccgaaaaa acagggaaac cctgcttgcg gactaagggc ggagaaagct gaggccagtg  92202 atatcaacac cagaaccaca aaagagatta cttcaatcag ccacatgcgc c atg caa   92259                                                          Met Gln            tcc tct  cta aac gcg att cct  gtc cac tat agt agt  agc cgc tca     92304 Ser Ser  Leu Asn Ala Ile Pro  Val His Tyr Ser Ser  Ser Arg Ser               5080                 5085                 5090                       ttt tct  gcg cta tgt gaa tta  gcg cct acg tcg ctc  agc ggg agg     92349 Phe Ser  Ala Leu Cys Glu Leu  Ala Pro Thr Ser Leu  Ser Gly Arg               5095                 5100                 5105                       aac gat  atg tcg cca aat aat  ttt gct cct gga gaa  caa ctg tgg     92394 Asn Asp  Met Ser Pro Asn Asn  Phe Ala Pro Gly Glu  Gln Leu Trp               5110                 5115                 5120                       ttg tcg  ccc agc gtc gga ctc  gca cga aga ctg tac  gga tgc gat     92439 Leu Ser  Pro Ser Val Gly Leu  Ala Arg Arg Leu Tyr  Gly Cys Asp               5125                 5130                 5135                       ctc agc  gat cgc cta tta tcc  aat ccg aca atg agt  agg ctg agc     92484 Leu Ser  Asp Arg Leu Leu Ser  Asn Pro Thr Met Ser  Arg Leu Ser               5140                 5145                 5150                       cta gat  cag cag cat ggc cat  ccg gtt acg ttc cca  ccc ccc tca     92529 Leu Asp  Gln Gln His Gly His  Pro Val Thr Phe Pro  Pro Pro Ser               5155                 5160                 5165                       cgc tcg  cga ccg gtg ttg att  gcg cgc gct ccg atg  gga tcc ggg     92574 Arg Ser  Arg Pro Val Leu Ile  Ala Arg Ala Pro Met  Gly Ser Gly               5170                 5175                 5180                       aaa act  acg gca ctg att gaa  tgg ttg gct gga ttt  cta gac cac     92619 Lys Thr  Thr Ala Leu Ile Glu  Trp Leu Ala Gly Phe  Leu Asp His               5185                 5190                 5195                       cag gat  aga agc gct ata gtc  gtg tca tgc aga aaa  agc ttt act     92664 Gln Asp  Arg Ser Ala Ile Val  Val Ser Cys Arg Lys  Ser Phe Thr               5200                 5205                 5210                       aac agt  ctt tgt cga cga ttc  caa cgg gat ggt ttg  gtg ggt ttt     92709 Asn Ser  Leu Cys Arg Arg Phe  Gln Arg Asp Gly Leu  Val Gly Phe               5215                 5220                 5225                       gct acg  tat cta gac tgc gaa  aaa tac ata ata gat  gag att tca     92754 Ala Thr  Tyr Leu Asp Cys Glu  Lys Tyr Ile Ile Asp  Glu Ile Ser               5230                 5235                 5240                       cac cga  cgg cta ttg gta caa  ctg gaa agt ctg cca  cgc gtc tct     92799 His Arg  Arg Leu Leu Val Gln  Leu Glu Ser Leu Pro  Arg Val Ser               5245                 5250                 5255                       agt gta  cta ttg gat cac tat  gat gtt cta gta gtt  gat gaa gta     92844 Ser Val  Leu Leu Asp His Tyr  Asp Val Leu Val Val  Asp Glu Val               5260                 5265                 5270                       atg tct  ttg atg aat caa ttc  ttt tcg cct aca gtt  aga aaa cta     92889 Met Ser  Leu Met Asn Gln Phe  Phe Ser Pro Thr Val  Arg Lys Leu               5275                 5280                 5285                       cgt gaa  acg gaa gct ttg ttt  tca tta ttg tta tct  aaa tgt cag     92934 Arg Glu  Thr Glu Ala Leu Phe  Ser Leu Leu Leu Ser  Lys Cys Gln               5290                 5295                 5300                       tat atc  gta gcg atg gac gca  acg ata aat gca act  ctt gtt gaa     92979 Tyr Ile  Val Ala Met Asp Ala  Thr Ile Asn Ala Thr  Leu Val Glu               5305                 5310                 5315                       atg ctc  gca gat ttg aga ggg  gca gaa aat ata cac  gtg ata gtg     93024 Met Leu  Ala Asp Leu Arg Gly  Ala Glu Asn Ile His  Val Ile Val               5320                 5325                 5330                       aat gat  ttc gta tcg agc ggg  ttt gcc aat cgt caa  tgc aca atg     93069 Asn Asp  Phe Val Ser Ser Gly  Phe Ala Asn Arg Gln  Cys Thr Met               5335                 5340                 5345                       ctt aac  gcg ctt ggc gcg gca  ata cct gcc agt ttg  tta aag ccc     93114 Leu Asn  Ala Leu Gly Ala Ala  Ile Pro Ala Ser Leu  Leu Lys Pro               5350                 5355                 5360                       tca gaa  gga aat gag gac aaa  gga ctc gag gat aac  aaa agt act     93159 Ser Glu  Gly Asn Glu Asp Lys  Gly Leu Glu Asp Asn  Lys Ser Thr               5365                 5370                 5375                       gca cag  gtg gat cca tct cta  ctt gaa ggg tcc ttt  ttt cat gaa     93204 Ala Gln  Val Asp Pro Ser Leu  Leu Glu Gly Ser Phe  Phe His Glu               5380                 5385                 5390                       atg cag  gcg cgc tta ttg cgt  gga gaa aat att tgt  gtc ttt tcc     93249 Met Gln  Ala Arg Leu Leu Arg  Gly Glu Asn Ile Cys  Val Phe Ser               5395                 5400                 5405                       tct acg  cta tca ttt tca aac  gtc gtt gcg ttc ttt  tgt tca gaa     93294 Ser Thr  Leu Ser Phe Ser Asn  Val Val Ala Phe Phe  Cys Ser Glu               5410                 5415                 5420                       att cta  agt cct ggg aca gtt  ctc tta ctg aac tcc  aac tct ccc     93339 Ile Leu  Ser Pro Gly Thr Val  Leu Leu Leu Asn Ser  Asn Ser Pro               5425                 5430                 5435                       cac gta  gat acg act aac tgg  ggg cga ttt cga gca  gtc atc tat     93384 His Val  Asp Thr Thr Asn Trp  Gly Arg Phe Arg Ala  Val Ile Tyr               5440                 5445                 5450                       act aca  gtg gtg aca gtt gga  ttg agt ttt gac tct  tgc cac ttc     93429 Thr Thr  Val Val Thr Val Gly  Leu Ser Phe Asp Ser  Cys His Phe               5455                 5460                 5465                       cac tca  atg ttt gcg ttc gta  aag ccc aca atc cat  gga cct gac     93474 His Ser  Met Phe Ala Phe Val  Lys Pro Thr Ile His  Gly Pro Asp               5470                 5475                 5480                       atg atg  gct gtc tat caa gcg  atg ggt cgg gta aga  cgt cta cta     93519 Met Met  Ala Val Tyr Gln Ala  Met Gly Arg Val Arg  Arg Leu Leu               5485                 5490                 5495                       cat gat  aag ctt ttt ata tat  tta gat gtt tct ggg  gca tgg ggc     93564 His Asp  Lys Leu Phe Ile Tyr  Leu Asp Val Ser Gly  Ala Trp Gly               5500                 5505                 5510                       gca cca  att ttc act ccc atg  att tta aac tcg gag  ttt agc act     93609 Ala Pro  Ile Phe Thr Pro Met  Ile Leu Asn Ser Glu  Phe Ser Thr               5515                 5520                 5525                       acc cca  tgg cca gta gac att  aca gtt cca gca gac  gcc atg tgt     93654 Thr Pro  Trp Pro Val Asp Ile  Thr Val Pro Ala Asp  Ala Met Cys               5530                 5535                 5540                       gta aaa  ttt aaa aac cgt tgc  aga cag atc aac aat  cat cga gac     93699 Val Lys  Phe Lys Asn Arg Cys  Arg Gln Ile Asn Asn  His Arg Asp               5545                 5550                 5555                       ggg gta  ttt acc cgg ttc aaa  aat aag cat tat gta  gaa aga tgt     93744 Gly Val  Phe Thr Arg Phe Lys  Asn Lys His Tyr Val  Glu Arg Cys               5560                 5565                 5570                       aca cta  acc agt gct aat gac  agt ttt agt ctc cta  cat act ctt     93789 Thr Leu  Thr Ser Ala Asn Asp  Ser Phe Ser Leu Leu  His Thr Leu               5575                 5580                 5585                       cta gtc  aat aac aaa ata aat  gtg gag ata atg agc  gtt gac tcc     93834 Leu Val  Asn Asn Lys Ile Asn  Val Glu Ile Met Ser  Val Asp Ser               5590                 5595                 5600                       tcc gat  ccg aaa ctg gaa aat  ttg ggt caa ttc ata  tct ggt ctg     93879 Ser Asp  Pro Lys Leu Glu Asn  Leu Gly Gln Phe Ile  Ser Gly Leu               5605                 5610                 5615                       cgt gca  gat agt tat cgg aat  agg gct cca ctt aag  cat ctg tac     93924 Arg Ala  Asp Ser Tyr Arg Asn  Arg Ala Pro Leu Lys  His Leu Tyr               5620                 5625                 5630                       tcc gcg  ttg gaa aag tat gaa  aag agt caa gat ata  ttt tgc ctt     93969 Ser Ala  Leu Glu Lys Tyr Glu  Lys Ser Gln Asp Ile  Phe Cys Leu               5635                 5640                 5645                       cca tca  aac cca act cct gag  gaa aca gct ttg atg  ctc atg gag     94014 Pro Ser  Asn Pro Thr Pro Glu  Glu Thr Ala Leu Met  Leu Met Glu               5650                 5655                 5660                       agt gaa  aat gta aac cag ttc  att gct cgt ttt ttt  gaa ctt gac     94059 Ser Glu  Asn Val Asn Gln Phe  Ile Ala Arg Phe Phe  Glu Leu Asp               5665                 5670                 5675                       cga tcg  cta gaa aat ttc ggg  gaa gac ttg caa gcc  ttt ctg caa     94104 Arg Ser  Leu Glu Asn Phe Gly  Glu Asp Leu Gln Ala  Phe Leu Gln               5680                 5685                 5690                       cag ctt  ggt gac aaa agc aaa  act ata gat gca cta  att aat gcg     94149 Gln Leu  Gly Asp Lys Ser Lys  Thr Ile Asp Ala Leu  Ile Asn Ala               5695                 5700                 5705                       gct gtt  gtg gaa aca gga tgc  acc tgc gat gaa gat  gaa tgg ttt     94194 Ala Val  Val Glu Thr Gly Cys  Thr Cys Asp Glu Asp  Glu Trp Phe               5710                 5715                 5720                       cag atc  aat aca gag gca ttg  tcc aaa gat cga gac  agt aaa ctc     94239 Gln Ile  Asn Thr Glu Ala Leu  Ser Lys Asp Arg Asp  Ser Lys Leu               5725                 5730                 5735                       gac tct  tgg ata tca tat tat  ctc gat ggt cca ttt  gtg tgt cta     94284 Asp Ser  Trp Ile Ser Tyr Tyr  Leu Asp Gly Pro Phe  Val Cys Leu               5740                 5745                 5750                       agc aac  gga cgt ccg gcc gtg  att gag gtt tgt aat  gaa acc gaa     94329 Ser Asn  Gly Arg Pro Ala Val  Ile Glu Val Cys Asn  Glu Thr Glu               5755                 5760                 5765                       gtt aag  aat cgt cga aac cta  tta aga cta tgc ata  aac att gcc     94374 Val Lys  Asn Arg Arg Asn Leu  Leu Arg Leu Cys Ile  Asn Ile Ala               5770                 5775                 5780                       agg acg  ata ggc tgg aaa cct  tca gcc gat cca aac  gag act gaa     94419 Arg Thr  Ile Gly Trp Lys Pro  Ser Ala Asp Pro Asn  Glu Thr Glu               5785                 5790                 5795                       att gaa  ctt gat gtt atc gtg  aag gca gta aat gac  gca ttg aac     94464 Ile Glu  Leu Asp Val Ile Val  Lys Ala Val Asn Asp  Ala Leu Asn               5800                 5805                 5810                       gca ggg  atg ggg aat tgt atg  cta gaa tat ctt cgg  tta aac ata     94509 Ala Gly  Met Gly Asn Cys Met  Leu Glu Tyr Leu Arg  Leu Asn Ile               5815                 5820                 5825                       aca gaa  ccc tca tgg tta act  gga cct ata agg aac  ctc cag tgt     94554 Thr Glu  Pro Ser Trp Leu Thr  Gly Pro Ile Arg Asn  Leu Gln Cys               5830                 5835                 5840                       tat ctt  gga gaa aaa aaa atg  tct cgc cgc gaa cgg  aag act tct     94599 Tyr Leu  Gly Glu Lys Lys Met  Ser Arg Arg Glu Arg  Lys Thr Ser               5845                 5850                 5855                       gac cca  cta gag atg gca gct  gta aag atc ttg cgc  act cta tgg     94644 Asp Pro  Leu Glu Met Ala Ala  Val Lys Ile Leu Arg  Thr Leu Trp               5860                 5865                 5870                       gcg gag  tta ttt gat gta cgg  gta ttt aaa agc caa  aag aca ttt     94689 Ala Glu  Leu Phe Asp Val Arg  Val Phe Lys Ser Gln  Lys Thr Phe               5875                 5880                 5885                       cct ggt  acg gca agg gtc aag  aac ttg cga aag gag  gag ctc tgt     94734 Pro Gly  Thr Ala Arg Val Lys  Asn Leu Arg Lys Glu  Glu Leu Cys               5890                 5895                 5900                       gct ttg  cta gac cgc att cac  gtt ccc tac gat cgc  aag gaa acg     94779 Ala Leu  Leu Asp Arg Ile His  Val Pro Tyr Asp Arg  Lys Glu Thr               5905                 5910                 5915                       cac aag  caa cta tat gct aaa  ctt atg tgt cat aga  gaa cag ttc     94824 His Lys  Gln Leu Tyr Ala Lys  Leu Met Cys His Arg  Glu Gln Phe               5920                 5925                 5930                       aag ggt  tct cga cta agt ttc  cgg act gct gcg tgg  acc cgt ttt     94869 Lys Gly  Ser Arg Leu Ser Phe  Arg Thr Ala Ala Trp  Thr Arg Phe               5935                 5940                 5945                       tta aaa  gga cgg tgc ctg gag  gac ttc cat ttg atg  act cca atc     94914 Leu Lys  Gly Arg Cys Leu Glu  Asp Phe His Leu Met  Thr Pro Ile               5950                 5955                 5960                       tct tcc  cat cat gta tga tgaaagcata ctttatggct acttatgggg          94962 Ser Ser  His His Val                                                         5965                                                                 catctcgata tatacgattg agactaaaca aaatgcgtca ggaaatatta cagacatgtg  95022 ggtattgtat gaattagctg tccatgattc tactaagggt gcccatgaag tactttatgc  95082 atccttacag tttgaagaaa gcgacctcag tgtggcctcg tggcctcatc cagctctgaa  95142 tgccagatta ctgacggact tcgttggggc tgtaaagaat ttccatcaga gggtgtacaa  95202 gctgaatgca aaaacaatta acttcggatg gattctctca tgttctactt ctagccagat  95262 tgctctccgc ctggtgacag gaagactact tcatgaaatc aggcgcgcct tgactctgcc  95322 agaattttat tctccttcta ttttttatgt ttgcaaagac agtggattga tcactaaggt  95382 ctgcgaggat aaatccaagc cacgtatggc aactgcatct tacgcagcat tgaatgcttc  95442 gtctaacttc caagcaaatt acattgaacg caatctgcaa gccggacatt gttgtgaact  95502 gagaaatttt ggatgggctc gcatacaggc aatctcatct ggaaaaatta atccaagaag  95562 catgaccgcg gaatgggtgt gggctggtgg aaagtggatt gatggaagag gaaatgaagc  95622 attttctgaa acagggactg atcccacttc cgacgatggc cttgctgtcc cctttgcctt  95682 aacacccgcg aaaacaattt atggaccgac gtggtttgtt cccagacgca tgcttgtagc  95742 gtcattggtg cccaaggagt tcgaatatgc catctatctc aaggatggaa gtacgataat  95802 ctctttagtg tgcgcaataa taaatctgta ttgcagattt taccaaggaa acattgttgc  95862 acaatctaca tttctcaaac ctattatcct ttttctattt ccgacaagtt cttcccgaat  95922 ggacggctca gagaaggttt catctataaa agactcctac atgtatacac ctggattccc  95982 gagtataaat tttgttccca ttacgacgca gaacatgtca aagatgggag ccttgaatgc  96042 atgcagaata gtctctcttg tggaggggtt atggccagcc tataatatca gagcattact  96102 catgctggat cgcggagatc gagcgaaaca tgggtctcag atcacaacac tacccagaga  96162 cctcgagagc actttagaag tgtacccagc cggtaaaata tccaccatag ccgatctccc  96222 gactgttatc agtggcagga ttttaaaaat ggacttttct gctttctttc catgtttgta  96282 catggcatgt ggcggaggca gccaagcgct atgccgcatt atcgaggcaa gattaaacag  96342 ggagccgcag tcagagaagc ttaaagctgc cttggtagct ctggtgggtg ggctaaaata  96402 tacagatcca tcgaaatata aattagtaat agcgctctgc aacagcattg cattggctgt  96462 cgaaaatgct gccaatagcc tccagtttgg aatagctatt tatatgaagg atggattcat  96522 aggagctttt gataaacatt cctctacaag cgcagaggaa ctgagaagca aatgcgaatg  96582 ggccgccatt gaagaattgc agaagattct tctagaatgc ggccaagcaa ttacaggaat  96642 gccgactctg aaactaaggc tagagggaga atttacagag ggcctgcttc ttaattgcaa  96702 caagtattgg ctccacaaca ggaataccgg aaagagtttc atatgtggga tacctgggct  96762 tcgagaagag aatgggctga gtgtgttaac agaacgtaca gcttgtgaac tgctcgctgg  96822 catttacact gccaacaccg taagcaccgc taccgaaaca ctcacgcgaa ttctagatgg  96882 atatgccttc tctgccttcg aagctagagg tgacataaac ttctggcaag agacgctaaa  96942 aagtagcttt accccatgca tttcagattc cgcagccatc agatctgcca gtttcatgac  97002 acgcccagat gagctggaag gagagactca ctttgtttac cttactccga caaatttgtc  97062 ctcccatggc tccgccaacg ctggcggtaa agttatctac ccaagttctc tcgcagaaga  97122 gaatttctgc attaaaatat gttattcggc tcacctaatc cccaaaatgt cagcaatggt  97182 ggagcttgta cagaatatgg tgtggttaaa attttatcat aattctgtgg acaatgatga  97242 ggagacacgg ggtaagttat tgaaaacgtg tgactacgat tacgagagaa catctttttt  97302 attctcataa ttaaacagaa catttccaaa gaccatgata cttggtactt cgtcatt     97359 tca aaa  ata ggt atc ata taa ctt  ata tgc act gcg atg  aag ctt     97404 Ser Lys  Ile Gly Ile Ile     Leu  Ile Cys Thr Ala Met  Lys Leu               5970                     5975                 5980                   gcg gtc att  cca cca caa tct taa tac  gcg agt aaa aaa gtt  tcc     97449 Ala Val Ile  Pro Pro Gln Ser     Tyr  Ala Ser Lys Lys Val  Ser                   5985                     5990                 5995               gtt gcc ata tac  ccg att caa agt tga att  cct tga ctc taa gat gat  97497 Val Ala Ile Tyr  Pro Ile Gln Ser     Ile  Pro     Leu     Asp Asp                    6000                     6005                                ggc  tcg ttc att ttc att  gga agg cag gtt cat  gat cat aag tgc     97542 Gly  Ser Phe Ile Phe Ile  Gly Arg Gln Val His  Asp His Lys Cys           6010                 6015                 6020                           gtg  ggt aga ata aag aga  aga tat tag ctc ttc tcc  aat tgg agc     97587 Val  Gly Arg Ile Lys Arg  Arg Tyr     Leu Phe Ser  Asn Trp Ser           6025                 6030                     6035                       ggt aat  ttg cat gag ggg ctg  cgc cac tcg ata atg  agg caa tac     97632 Gly Asn  Leu His Glu Gly Leu  Arg His Ser Ile Met  Arg Gln Tyr               6040                 6045                 6050                       caa ccc  ggg atc aaa ggg agg  aag atc atg cat taa tag caa  tgt     97677 Gln Pro  Gly Ile Lys Gly Arg  Lys Ile Met His         Gln  Cys               6055                 6060                         6065               att taa gag cca ctc  ccc tcc att cac caa  tag ctc gcg cac ctt      97722 Ile     Glu Pro Leu  Pro Ser Ile His Gln      Leu Ala His Leu                            6070                 6075                                atc  att gat tga aat agt tcg  cgc gaa acg ggc aat  tgc gct tcg     97767 Ile  Ile Asp     Asn Ser Ser  Arg Glu Thr Gly Asn  Cys Ala Ser           6080                     6085                 6090                       taa ctt ata  gca gtg ttc ttg agt  aac ctt aat ggg gtc  aat gtt     97812 Leu Ile  Ala Val Phe Leu Ser  Asn Leu Asn Gly Val  Asn Val               6095                 6100                 6105                     ttc caa ata  cat gca aat caa aca  gag cgc aaa gtc ttc  aaa ttt     97857 Phe Gln Ile  His Ala Asn Gln Thr  Glu Arg Lys Val Phe  Lys Phe                   6110                 6115                 6120                   ttg ggg cat  aat tct gtg aat cct  gtt taa aaa atc agg cca  tgt     97902 Leu Gly His  Asn Ser Val Asn Pro  Val     Lys Ile Arg Pro  Cys                   6125                 6130                     6135               aac atg cac agt  ggc aac gcg gtc ctc  tga ggc tgt gat tac tga      97947 Asn Met His Ser  Gly Asn Ala Val Leu      Gly Cys Asp Tyr                            6140                 6145                                    aaa  tat aaa gcc agc atg  atc atc tag gcg cac aca  cgc gca aaa     97992 Lys  Tyr Lys Ala Ser Met  Ile Ile     Ala His Thr  Arg Ala Lys           6150                 6155                     6160                       gta ctc  ttc aat atc cac tac  tgc tcc aga aat cag  ctc tag gat     98037 Val Leu  Phe Asn Ile His Tyr  Cys Ser Arg Asn Gln  Leu     Asp               6165                 6170                 6175                       aat ttt ttc  ccc atc cat ctg gat  gtc cat ata tgt tat  gga atc     98082 Asn Phe Phe  Pro Ile His Leu Asp  Val His Ile Cys Tyr  Gly Ile                   6180                 6185                 6190                   aga cgc aaa  caa tgc tgg cgc ccc  tgg cgt gga tcg tac  ttc aaa     98127 Arg Arg Lys  Gln Cys Trp Arg Pro  Trp Arg Gly Ser Tyr  Phe Lys                   6195                 6200                 6205                   aaa aaa tct  cgg gat agc ctg ccg  cga tat ttc ctt aat  taa ctg     98172 Lys Lys Ser  Arg Asp Ser Leu Pro  Arg Tyr Phe Leu Asn      Leu                   6210                 6215                 6220                   tga tgt cga ttc tcc  aga gga ggc cag acg  ttc aag ttc ctc aag      98217 Cys Arg Phe Ser  Arg Gly Gly Gln Thr  Phe Lys Phe Leu Lys                        6225                 6230                 6235             ggt agg gcc gtg ttc  ggg ttc ttc tgc gat  gtc ttc caa tcg tat      98262 Gly Arg Ala Val Phe  Gly Phe Phe Cys Asp  Val Phe Gln Ser Tyr                            6240                 6245                 6250             agg tcc tac ttt ttt  ctg atg ccg gca tat  cgc gtt gaa tgc ttc      98307 Arg Ser Tyr Phe Phe  Leu Met Pro Ala Tyr  Arg Val Glu Cys Phe                            6255                 6260                 6265           ttc ggc cca tga tac taa ctt  tcg agt cgg ccg att  ctc tgt cgc tgc  98355 Phe Gly Pro     Tyr     Leu  Ser Ser Arg Pro Ile  Leu Cys Arg Cys                                6270                 6275                        ctc  tag aat agg ctc tcc tgt  ctc ttt cag ctc ttc  cat ccc atc     98400 Leu      Asn Arg Leu Ser Cys  Leu Phe Gln Leu Phe  His Pro Ile           6280                     6285                 6290                       gtt cat  ctt gcc gat tgg cgc  acg aac gtc tga cat ttgctatgta       98446 Val His  Leu Ala Asp Trp Arg  Thr Asn Val     His                            6295                 6300                                            acgtacgttc agatctttca aatacgaacc tacccgcgag ctggcaaata atgtttgaca  98506 caactcctcc tctgacttat atgtagtctg gctcgataca aggaatccta gacgtgacgc  98566 cttcagaact ttgtgaaaaa atggaccgac aataagcgtg attgcgctag tactgtacga  98626 caaagatact tcctcgcctt gattattcat atttctttga attttaaaca cgcgcagtaa  98686 ttcttgttcc cagagcgccg acaatcgagt ctcctcctcc agataaggag gaatgtaccg  98746 cgccagaaaa ctattagcaa tatactgttg gtcattcatg tccttactta catcaacgag  98806 gttatatgat aaattctcag ggagcttcga tagcgactct agagtttctt tgcactccca  98866 agcctcttcg atacctccaa gggtctccca tgctgggcaa cggtcatagt ttgcttgggt  98926 gcgctcagtc cgcctggcag attccattct agcctcaacc aattcttttt tcattttccc  98986 attactatag accaagtttt tattgctttc cttgagatca gagacagtct tgaacaaagt  99046 atttactacc tttccaacac ttgtattaac ggcagctcgc acagcattcc ccatgcgatc  99106 ttctgcatta tcaatgggcc gctgcccctg cgctgccgct cttccgccac acccaacacg  99166 ggtagggtca agcacgagtt gtctctcgat gttagaaaca ttttcacgca agtaagagtc  99226 cactacgtct gccacatcac caactttgcg catattttga atatcaataa tgaacttgat  99286 cagcctagct gctgcagatg taccggcctc ttccacacct tctcccagag ccttatccac  99346 cacttttgtt acatgatctg gatcctgtgc attctcagtt cgtagaccaa gcaaaacctt  99406 gatcggagcg gtatttacta attgacacaa ttttgcatgc tcgcgcatag cctgagcttt  99466 gagaacagga ccgtgcaatc gttgcatagg agaatcaaat aatactcccc tgtcagttac  99526 caatggttcc caaacaacca tacattcccc aagctcccca gtatttccat cataggcgcg  99586 gatttcactg gttttaacgt tcattattat tagtgttcgc tcatactcta aaatatgtac  99646 tgcccctaat acagcacgga gatacttcag catttcgaac cctctggcaa aaagtgaagg  99706 cgcgacatgt aggtggtggt gtatttcagt cgttgtactt tccaggtctt catttccgag  99766 atcactgcca gtggggcgct tttcttgagg ttttctttct cgtaccaaag gaaccatacc  99826 cagacatgtt atccaatcta tataacgtac gtaggaagga gccttcccag cttcgcttaa  99886 cccgcgcaag cttaactcag acacgccccg tacaaaatca agtaacactg tctgcaataa  99946 aaaggaccac aactcgaacg cctgctctgc aactcgaatt ccatcttccg aattgtgaca 100006 gtaacgtgct gccagttcat ccggtgtcaa ccctcttgtt ctgatgtgag tgtcccaatc 100066 gcgctccaca tcctcaaatc gacagccatc caatatattg cggagaaccg ccacttgaat 100126 ttgtctaaca acagattctg ttgctcggac cgagttatac acgccttgtc cagaagtgta 100186 cgtcgatgcg cccaggagaa tttccctgaa tcccatcgtg cgctttgttg gatggattat 100246 tatccagtca tcaggctcgc ctgttagaat cggctccaag accgcgataa ggttgtcgat 100306 atcaaaggca caaggtacct gacgcaattg ggaaaatggc atgtccattt tcggggaaga 100366 gcaggcggga aatagaaatc accactcctg tttacttaaa tttttcggca atgcagagca 100426 ttcaggcgat tttatcggac attacaatcc tatcagaaca agctgttcat gaagactcca 100486 agccgcaact gtcttggttt gagactgtgg cacgtttaga tgaaccgacc actttgcctc 100546 tggcggaatt accatttaat gtttatctaa tcaccggtaa cgccggctca ggaaaaagta 100606 cttgcattca aactcttaat gaaacattga attgcatcgt tactggctca acgcgcatag 100666 ctgctcttaa tatcttcaat aagctatctg cctcttacac ctcttgtccc attcatacta 100726 tttttcaaaa ctttggcttc aaaggaaaca acgtacaggc tgtgctagga cgattcaagt 100786 ttgaaaaacc tcacgaacaa cgttcgttgg cagaacatca aatggccgac atttattatt 100846 attgggacgt gataaaggac attactgcac gcgccataga cacggcatct ccggtagctt 100906 tgtctgtcct tcaaacactc cagcagaaaa cttcacgcaa atttgtaaac cttgcacctt 100966 tcctaatttc ctctatgcct ccatttgtca aaagcaacat tatccttgtt gatgaggctg 101026 gagttttagg gaaacatata ttgactgcaa tagtatattc gtggtggctc atgaatgcat 101086 tgtggaaaac gcctatgtat caggacggga agaagccagt gatcgtgtgc attggctccc 101146 ctacacagac agacgcaatg gaaagcagct tcgagcacag aaatcaacga caccttatat 101206 ccagcagcat aaacatttta agtaatctta tttgtactcc tacactgttt tcagtcctga 101266 atatcaagaa gcagtgggcc atctttatta ataataaacg atgctcggag cctgctttcg 101326 gagaagtatt aaaggcattt gaatttgggt tacctttgac agaacgccat gccagattct 101386 tagatcagtt cattgtatca gaatcattca tcaaagatcc atcaaagctt cctggatgga 101446 ctaggctttt ctcgtcccac gaagaagtta aagaatacgt ttctaaattg cacgcaaagc 101506 ttcgtgcaca gaaatctgaa aaatataggg tatttctcct gccgatgtat acaatagtag 101566 atatggcggc atttgaaaag tataaatccc tgacgggtca agagacacta aatattgatc 101626 gctggctgca aaacaattcc tctcgccttg gaaactattc acagagcagg gatctagatg 101686 tgaccgcgcc cagatttgaa taccatcgag atgagagtaa tgaatataca ctagtaacga 101746 ctgatgcctc gcacgtactc aatagccaga tcacggtaac aaaaaaggta aaaaaattga 101806 tcttcggttt tgagggaaca tttgaaaaat tcgcacatgt attatccgaa gatagctttc 101866 tcaagacgta tggagaagac aaagtagaat ttgcctatca ctttctgtcg accttgctat 101926 actatgggat gattaaattt tacgaatttc tccgaacaga gggcctcccc gaagataaat 101986 taacaatggc gtatgacagg ttgtactccc tagcaatgcc ggagccagaa actcagaact 102046 ttgacatggg aaatggtgaa aaattttgtt ttaacgaaga tgaatgtccg aatgccgatg 102106 tgaatgacaa agatgattta ttcgatattt ttgataagtc gttagatcaa ttctatctaa 102166 attatgagat ttgcgggagc gatgtccacg gtcaagaaat atttagttat ttcgagcaga 102226 tgaaaagaat ttatacttta cgttacgcgg tgctgtgtga gctctttggg agtgtattta 102286 ctgccgcccc ctttagctca ttcgtcggaa cggcttcttt ctcaagtcaa gaaatttcta 102346 tctccagttt caagggagca gtatgtgcat ttgctgctca aaccgacaca tataccttac 102406 gcgggattac gcgagcaaga tttccgggat atgctgagga cacctcaaaa gcccacgaat 102466 gggccgagcc aattttacaa atgctagact tgccaagact tgtcgtaagg gatcaaatgg 102526 ggttcgtttc agtcctgtgc cataacaaag caacttttgt cgataatata ggtggacagg 102586 aattaaggat ggccataaca attgaccacg gaataagttc gagccttgca atgacgatca 102646 cacgatctca agggttaagt ttagacaggg tagcaatatg tttttctcac ggaacaatga 102706 aattaaatac tgcatacgta gcaatgtcta gagtaacgag tagcgaatat ttgcgcatga 102766 acttaaaccc cctgcgaacg aaatacgaag atactcgaca agtaagccaa cacattcttc 102826 gcgcgctcag atgcaaagaa acgcgactag tatactaatc ctcaaaagta gccacacgca 102886 tggctttaaa atcagtttcc caccgtcgtt tggagtgttg cataccctcc tcgtcccgag 102946 aacatgattt tcataagcta cgtaatggta ggagtccaag gcttggggca tggctcggcc 103006 accgaatacg aacaagtagt atactcatgc gatggaggaa tgcggtttat ttgcatcgga 103066 aataaaatgt atcgccacca attaccaccc gggaaagtaa tagtaattca caatcccgtc 103126 gcaacaatga tcgctgtaga ctgtgaagaa gaattttgcg catactgttt ggagcgcaat 103186 ggttctcacc gaggaccctc aggagaaaca ttagcttttc agttctcggc atgttggttt 103246 ctagggcgcg gaggaacgcg agaacggtgg agcagcggca acatcaccat gatgaatttt 103306 ttgggcgtcg ctcacttaac tgtaaccata tacgaaaccc cggaagatct agcatctagc 103366 gcgcacacca cccccacatg ttcccaatcc tctccagaat cgctggagtg tccagaggaa 103426 agcgttcctc gagatcttgt agagtttgcg gcaaggcatg ccggactgtt agaggaataa 103486 ataaaagtaa taaacaccaa attatgaaat acgagcattt aattattgcg tcgcattgcg 103546 gaaatgagtt gaccttcata gttgctgtca cttagctgca gctggaggta gtagtgagga 103606 aaatcgggtc tcgaaatgtc tagagggtag ctcaggcaac ttttccgact tcggtagaaa 103666 gcaatatcca ttaccctgta acgcagccgt ccgtgccgaa gagcacctgg agttcttccg 103726 tggaggctgg cgagacatgc gggcaaagtc catatcagtt gcaatagcag cctgaccgta 103786 cttcatagtc caggaagtgc aatattcgcg ttctgagtaa ctttcgcaat ggggcaggta 103846 taaatgttct agcgctgggt tccacccaga ggcgcaccca cgtaagtcaa cacgccattc 103906 ctccagtcat atgcctggcc aatctgcgac aggcatgggc aagattgtcg cgtggaataa 103966 ataccggggc atgggctaca aactcgtgaa agaaaactgg cacaccgggg tgagtttctc 104026 catcctcgga ctgcgtaaaa tattttcttg actttcttga cgaaataatg tcttttaggc 104086 gagcccttat ttgcggggca tgtcttctct tgcaaaagag aaacatctgc aaacttttct 104146 gatgtctatt tgcacctgca aatgcggacg tcttagtaca tgccatcgga actctgtcta 104206 gggaagtaca aatgtctttt cgaatgttat cggtaagctg tcttctcccg agttccccga 104266 atgaaaatat catgaaaaca ctgtcaaagc cacacgagtc caaagattct acatcttcgg 104326 tgttattcgt tttgacatgc ccttggacgg ctacgtcttt tgcgtggtcc ggagtaacat 104386 gagaatgcgt atcatgacat tcgtacgatc cggaaacaca gtcaatagac gaggctccat 104446 atagaacttc tgtcacagag gggatctcgg tacccatatc agagaccaca cgccaccaca 104506 gagaaaggtc ttcaatttat tgtttatctc gcaattttac atatatttta acagcaacaa 104566 gctccgcccc aaaataaaca ccggcggaaa aaacatacta atctaaattc cagtcaatcg 104626 gtgacaggcc cttagattcg aagtattgat ttgcttcctc gaaatggcga cagttgaacg 104686 gacgtcgcgt gcaggtagaa ggatggatga actccaactt taaatgattt ttaggcacgc 104746 ggggaataaa tcgtctagct tcttggcccc aaagcatcaa caccgtagat gacaactttt 104806 ctagtagcga tgagatcact ccctttgtaa atttgtccca gccgatggtt gagtgcgatc 104866 taggaaaacc ttgttccacc gtcaagtatt tgttcaaaag caatactccc ttcctggccc 104926 aagattctag acacccgtga gtttcctttg gaatagatgg gtaacagtcg cgtagagctt 104986 ccaaaatgcg gcgcaagctg ggcggtatac ggcaccccct agggacactg aaagctaagc 105046 catgcgcgtg tccttccgtg ggatacgggt cttgcccaac gataataaca cgcacatctt 105106 gcggattgca gtatcttgtc catgcaaaaa tatctccttt aggaggaaag attttgcgga 105166 atctagaaca gtaggagtac tgtaacagcg tctcaccgaa atcttcattt tcaattagag 105226 gtttcagaat tgactcccat gatgatggta ttgaaaactc tatggccacg tcggtccact 105286 ccggtacgag cttcctcgcg gatggtataa ccaattggac ggaagtcatg ctcggagggg 105346 ggagagtacg gcgtttttta gcagcgttgg ggtcggtatc tctttggttg ctttcagtag 105406 gagagctaat gtccagcgga cgcttgaagg gactttttat gggctgctcg catagtccgg 105466 gtgatagtgg ttgatgttcg tttccggcca tctgaaattt atggtggtcc ttttatacaa 105526 cgaatcaaca agtccacgag ccaagacgtt agcagccgca agcccggtag cttccagccg 105586 gtcttgaaga agtctaatcc ccaagtacgg attcactttt gcagcgtacc cattcttata 105646 gtagaaaaca acttccgtgt ttacgcacag aggtctgtaa ataatcacag tcaaattttc 105706 aaagtagtct cccgatggca acggcccgaa aattcgcgca ccgttatctc gcgaattact 105766 atcacatggt gcggccgcgg cagaaacagg atcacgaaaa atgccaggta tatcgacact 105826 tgaacatagc ataagagatg ctattagaaa aaaacaagtc aacattttaa aaatcattat 105886 catatggaga cctaggtctc cgttcacgat ccgtgttacg tcgacgagca gatggaactc 105946 tttgcgcccg acttctaaat ggatcgtcgt cagaatctga gtcgtccgaa tacaaatgta 106006 gtggtctaca ggaaccagta ggtactcttt gtggcggact tttgaatgga tcgtcgtccg 106066 aatcagagtc gtcccaatat aaatatgacg gcctagatgg agtacttcgg ctctgtagtc 106126 ctgaaggacc ttcacctgcg tcatcgtttc gcatgcggag gctccgattc tgcatggaat 106186 cgaggtctaa aatacctctg tctgcggcga cgggggattc gggttcattc tttatccccc 106246 catgacgagt ctgtgtacca gtcgtatttt gccctgcgcc atcataatta gacgaggggt 106306 ggtggggcga aagctgtgga ggggagcgtc tagatggcga ggggagggat gataacctga 106366 ggtctagggc cgtaggagat ggcgcagaaa ataaaggggg tatagaggac ggacgtgcgg 106426 gcggtggact atgagcgggg ctattttcta tttcatgccg cacgacccgt cgcacccacg 106486 acatctcata tacatctcgc acttctgctc tactcgcaag gccatagttg ccttccccct 106546 cttctgtctc cggtaacata aacgtatcgg atacggtggt cggccgcttt ttaaccattg 106606 gatgtgcgag cccaactggc ttgacgtccg tgctgtcatg agactcgcct ttgacaggaa 106666 ggcggatcga caccatcccg gcggaatttt taaaatattt caaatccacc gtgagtggtc 106726 tggtcagcat gtcgggctgt atcgtctcag gaatggccat tcctaccctg tactctagac 106786 ccgatgcact ctgtaccgtc ggggtttgtt cgtacgccaa aaagctatca tatccgctag 106846 ggtgattcgt cgtaaactca cgtatccatc cattgatcct ggttagaaac gagtaaaata 106906 cttcaagcat ctgttcttca tcctggaatg gcaattccac tgcgcgtagt gcggaatttg 106966 ctccgcgcgt caccagatga cgcgcgtttg agatttctgg agctgaggcg attgccatac 107026 tataaacgac acttttagcc ctcagtagag tagaatacag cgcctcaaaa tttctccacg 107086 ttagttgacg agttacaaac gtttcaagca ttatcgccct caaaacgact acaacgtcat 107146 ctatggggga tagccttccg gtatggtgtc ttcgagttac tggtctttcc tgttccaaaa 107206 tagtctggat ggcttggcgt tctccatcgg taataggggt accggcctgc cgcctggttt 107266 gaagtaatct cctaacgctt tcaactgttg tcggaaaata agctacaaca gaaaccaaac 107326 cattaatcct cgtacctcaa aaaatcttac acgcgtcatc ccaccccaca aaccacttac 107386 gcctcatcaa gacccagaaa tccatatagt gcgcttcatc gatgtccgcc acgaatgcta 107446 tattgaaatc tccgttcatt gtcattcttg cgcgcgattc tttactctct ccagatcact 107506 aacgcgctcg ccgttgcact ggaaagaata tccgtgcagc tccgacgcac gtccttatta 107566 gcccccgcgc ggatacacat gcgcgacgta acggtatatc cgagttttcg gattagttcg 107626 gtagcggcac actcacccac ttactaatca aataaacaca ccgtacgcgc caaaataata 107686 aacattcgtt tattgatact ttgtcagtct ataaatcagg tccgtcgatg gatctggatc 107746 ggttcctaga cctggacctt aactggtatc tatccctgcc cctgtgaggc ccaattcttc 107806 tatcgtccaa acctacattg cgtaatattt cacgcgccct gcggacacga attaaaaata 107866 cgcgcgggtc cgtcgggcgt tgcatggggt tgttgttttc tccttcatcc gcgctcaaat 107926 cagattctga gctcaatacg atagtctcta ttggttcctc cctccgttgg gggtcgcggg 107986 gttcaacctc acgcacatca gccagcggct gcgggctctc ggccctcccg ccctgtcccc 108046 ctccatgcca accctccacc atttccggcg gtgggtcgta ctcagagtaa ttgccgtctg 108106 aatcatttga ttcggaacta agctgctcat cttcggctcc aaatggggca tcgtctgcgt 108166 cttccatcag cgcttcttgt actgcagcgt cgttcattga gtcatcgcta tcgttttcac 108226 ttgtggacag tcgggcatcc tcactggagg aggagtgccg gtagtggacc acaaattcat 108286 tatctacagg tgctcggaga cgccagctct ctgactgtgg tatagccaca ctcacgttga 108346 cgggacgtac agagcttacc agggggtccc cagcatctcg gacaggcagc ttaattgtca 108406 cctctcccac agagttctta aatgcggctt ccctgcgcga cttatagcgc tccggagctg 108466 caggtcgcac cctcacaagc gtgtcttttt tgggcatctt tctagggttg acgtaatacc 108526 tcatctcatt ttcctggata tttgtgcgat gctccaaata gcctatgtca gctttgtgac 108586 cgcgtcgatt agttctactc ttgaacttac gacaccacct aatggagtgt cgcaggaatt 108646 gtaggaaatc ctctgccatc gactctgggt cacgtactgg gagaggaaat cgtaggaaaa 108706 tttcgtatgc gcccccgtac accttttccg ttaggagctc gggctgattt gtacgtgtca 108766 tgatatcata tactacctcc ttaagtcgta caaaagcctt tagtatgtac agaatgtgct 108826 ttatcgtcaa tttgtgcagc aatagcatct ccatcgtggc ggctttcact accatcaggt 108886 attcgtctac aggagacatg cgacccgact ggtgtcgtct gatgacggtg cgttcgttgt 108946 ccagcacatg ctgaattgca ctgtggtcgg atgcagagat cttacgcccc tcttccggat 109006 tttcatgtct cgctcggacc tcccctagct tccccaactg ttttacgtca tgcattctgg 109066 taggaaagag cgctagcaaa aagaaacaca aaaacaatag tatggtggtg tctcacattt 109126 gcccatgtcc actcaggcac ccactaccca tccacatcac tcgcccaagt ttcccttccc 109186 gcccggctct tacgttcttc tatctccagt atgcgttccg ttaactgggg cgtcaactcg 109246 agttctaccg tcaaatcttt ctgcacgcga ctaagatttt cctcgtcacc cattcgcgcg 109306 aaatttatcg ccatgccccg ccgggccgct gccatttcaa cgaacgggcc cgatggcgct 109366 gtgggaagag caactcccga cgcgtcgcag cgatctaccg tctattgcaa cgaggtgctc 109426 gatgcgctgc ttcttaatga cttgcccatc gccgctgccc gctctgtact acctttcttt 109486 cattttttcc gccacgcgaa agtgaccatt gatgtactgc attgcgtcgc ggaagcgcat 109546 gcagtgccgc gcgcgagggg tttcgaatag ctccacagcc ccgtcgtcgg caataaaccc 109606 gggggggtgt tttgcggggg ggggctatat atgagcagcc aaggatgcag ggtgtgtgca 109666 ctggtggtcc gcggttttct ccgacccgtc ccctcccagt cccctgagtg ctccccgccc 109726 ttccaatccc ttgtggtttc atgtccgacc cctactatta gatgctggac aggttctggg 109786 gttgcgagat acttgacggc ccgacatcaa acgccagcgt ccgccaaccc ccccccccaa 109846 gttcttccac ccgtggagag caaacggtca gcgcgggcag ccgtgaaact agttctacta 109906 gattttccag tttagccttt tccggagctg aaaaggttca ggtgggcacc ggtgggctcc 109966 gactggacct cgaagcgcga tcacagaatc gtcacgcagt tcgccccgtt tgtaagtttc 110026 gcggcagcgc gaggtgcttt gctcagtagt catacaggtc acgcccgtgg ttcttgcaca 110086 ataagcgcca cactggaatt ttcgatttcc agcaacgctc actggcaaga acggacacga 110146 acgtgaaggc cccggggggg ggggtcttat tccgtataac cgcctcacta tgtggccacg 110206 gccggttacc gcgagttata atagtcaagg attgaaagaa gacagagcgg ttgggtagct 110266 gacagttgtc aggccgaagg taatacgcgc tatcgatgtg aaagctatcc gggtaacgac 110326 accttggcct aaggccgcaa ttaggtgcta ctcaggttaa gcacttagct aagcgcagta 110386 gcacctaact gtgcccgcac ttatctttgt agagtgtcgc ggaagcaccg tgctcccagt 110446 ttctggtatt cgtaagcacg tgctgtgagt ctcagaggtt tcaaaaattc tcgttctggt 110506 aagttgttca cgcgcaaccc acggcgggaa aattttctgc tcttgcaagg gctctagtgt 110566 agccgcttcc gggtatagtg ggggctaggg ctttcccgcc acgtaaagct atataaatgg 110626 tggcagtgta tccttaggga agaacccaga gggagaacta ggggagaagt ttcgcggcgg 110686 cggttgcgcg cggtgggaat gcctggcaag tcgaagcctc cattgtccgc cgcactggcg 110746 ttactggtgt tactacttgg agtcggggat gctcgcagta tacgcgcggc acccttgaag 110806 ttactggcgg agagcatcag aatactgcac cgtgtgcaga aggacgtaag gcaactcatt 110866 ctcgttggac catgctgaca tgcctgcacg gtctttgtaa ctatgtacgg ctattcagtc 110926 tatgttcacg ttcataactg taggctctct cgtttcctta taagggttcc tgcgacggta 110986 gaagagttcc gaacatcttt acgtccgaca cggtgagtaa aagccagctc atttgtcaca 111046 tgttctcaac ggcaatgccc tggaggccgg ccgcgcgcta gccccagatt aaaaatgtag 111106 attttttgct taggtctacc ctgcccaata atctgctcgg cggtttatta acgaatcttg 111166 cttttcctgc agcgtctaaa cgaaactgaa gagttgtgcc aatccctttc ggtcattttg 111226 aaacaccaac agtgtcacaa aaaactgcag gggctctacg tcaatttgta ttacgtgctg 111286 aattctgtca gtaatggcgg tagctctcgc aaggtaagat aatggcagca tcgcgcattc 111346 tgtactttga agagactatc taacgcgcag cacagtatct ccgagacatc ttttgtctcc 111406 gtgctcacgc ggtccttatt ggtcgcatga cgagtctaac attgtatctc ctatttatta 111466 caggcaccgt gccctataac agtggaggat tcgtatttga tgaagtcgtt cctgaatgac 111526 ctggcgaacg ccctacagag acgttacgtc cgtcagagca ggcaatacta gacaatctag 111586 tattttgatt gcgattttta tatcggcgct tgcaatagta ataatgctgc cggccagcat 111646 tacaagcttc cgtggatctt atgtatcaaa atgaacgaat taaatattca agtgcattac 111706 ttaacacctc gttccgcctc ctcccgatat tcgtacttgc ccccattctc gtcccttttc 111766 taatatcact ccatggccgc acgggtagaa tgttcagcaa agctaaagtc aacggggcta 111826 gatcatttcc ccggtctttt tctacaagtc gtcggtggct gacaagctcc gactagcaaa 111886 agaatgagca cgcaaccaga agtaaattgt acaaaaagtt ttaataagtt aagttaataa 111946 cggaaaccac ggttaccacc aagggtcagt gacagtggga tgcggtagag ggtagttagt 112006 catgcgtcta tggcgttgac gcgaccgcct atcctctgaa gagtctaatc tgggtacaag 112066 ggttctagtt tcccccctcc ctgaggagac ggttagcctt gggcgcctgg tgcgcgtgtg 112126 tccggtgcat ctgggtgact ctggcgttgc tccccgcgga gcactccagc aacaacacat 112186 tggggaccga ttgcggggag tgtgataatg ggaatagtcg tcaccaagta gcaatgcgag 112246 aggggcggga tttctggcac gcataagcgc tagtccttcc acgtgaagcg ggttccgacg 112306 cgcccgcgcc cctggcgcat ccgggtaata atcggagatg atgcggagaa cttcctgtgg 112366 aattttgctg ggatcggacc aggacgctgc gagagggagg gcgggaatta ccgtgtcccc 112426 cccccacgca gcgacggccc taaaggaagc agaagtgcct cccacaatat cagcacaggg 112486 agtgcgcacg cggtagtccc ggccacggta cacgcggtcc cgaatcggga ggcgctggcg 112546 gccgcgccgc tttccacctc cggcgcgctc acgatcttct ttctcgttct tctcgtcccc 112606 gtcttcttca gatgagctgg agtctgaaga agacgacgac gaggagcatg aggaagagga 112666 agacgacgaa gaagaatgca tagtggaact ggactcggaa aggtccggac atggcggcat 112726 cgccccggcg cccacatggg gctgggccat gtacggatac aagacggctg aagcttttcc 112786 gatttcggag tggctccacc cggcatccat ggcacgagca acgtcgcgta atctcctgag 112846 agttacgtga gtgaggaagg ggtgcttaac gggaggacac ttctctctgt ctcctgtacc 112906 ctcatcgtcg ttgtcggtct tcccaacgct gaggggccat gagacagtga gtcgcctgtc 112966 cattactacg tgacctacat tgagcccacc ccgttccaac cgttcggccg ccggaccggt 113026 aaacagtttt acaaggtaag cgtgcgacgg ggggctagtc cgggcataga caacacccct 113086 ccgcccggta ggtatgcgcg gacccgagcg ggaatcctcg tccccgggat ccctagcatc 113146 tacgaccacg catgcgcgcc cagatgtact gagagcgcgc tcaagcatcc attctaccgc 113206 cccggcgtat ccaaggtctg tcgtacagag aatggccacg ctgcggcccg ggtctttctc 113266 gtcgggaggg atatagggat actccccgcg tgtccacgat tctggccaac aggcaccgcc 113326 cggcatgcag gcacgtccgc agagggccgc gtaggcgtac gcgcgttcta aaaattcgcc 113386 cggggcacgt tctgagacaa gaatgatgag gtcatagatt ttgtcagccg gatgtcgcaa 113446 cgtccaggaa agccgctcct ctaagacgca gcgcccccct ctgttcacgg gtgaaatcct 113506 cttgcaaaac tcccccaggg ctactgggtc cctcgcaaaa gcaggtagcc aatctgcggg 113566 tccggtcgcg gaatagcttc ccgccggacc gctgctcaac ccgcgcaaat agttcgcgag 113626 ccgctccaaa gcaggacgcg cgcgatgggc gggcacgtat ggctcccctg cggagagcgc 113686 aacgcccaag tttttgccat ggggacggta aacatcccct tggttcggga tgaaaccctc 113746 cggtgcgaac aggatggtag ggctgattgg ggtagaatga aacagggcct cacctatctc 113806 ggaaatgtga aaggacgggg attgtggttc ttgcggttct tctcggggcg gacatggtgg 113866 accctcggga gttatggggc atggcgaaag gaggaggttg tctctatctg tttctggaac 113926 cgctggaggt aatgaatctg gtaagccctg gaggccacat gtttttttag tcgcgttgcc 113986 ttctgggatc gggtccgcgt gatcttcaga tgactttctt ttcagacctt tacgggtctc 114046 aaagtcatca tctggagggc ggtcgggttc gggcccgtac ggtgacacag ataatggctc 114106 ctgttcatcg cacggtggag ctctgggcca ggggttggcg ggagattctt gggcagccga 114166 aggctctgct ccgttgccgc cgccaggact cccgtagagt aggaggtggg ccgcgcgatc 114226 gggagtgcca ggaggtaggt cgtaccggca agtgctccgc cggccgcctc gtcttttgcg 114286 cctgtcatgg tggtgggtgt catcatctgc gggacaatcg tccgtgacgt gctcgctgcc 114346 gggcgcggaa tcatctccta gcatggtagc gatcatcctc caaccagagg ccgcgtaggc 114406 aattgaaggc cgcacgtcac cctcgtcgga gcagccaagc acgcccacgg cagcttccat 114466 ggcttctaca agaagagtga catcatcggt agatggcagc ggcagggacc ctatgacgag 114526 gcgactccat agcgaagccg cgcagtagcg catggcgcgc tcccacgtaa agactgtttt 114586 gggcgccctc gagagtgctt gatacagatc cgggcgacgc ttggcggggt ttattgtaac 114646 tggtgggcag tctatagcag taagcgccgc catgagatct gcggcagcaa caatgcaagt 114706 tctagtatgg cgcaatagcg ccgaagagga aagtttttcc ggggttgccg caggtggctc 114766 tttcgctgcg gcttccagct ccctcgtggc acgcgctatt cgcctggcgc caacgagtac 114826 agggtggtcc ccagaagcag acgccgccgt aggatccacg gccgctcctg gaaatacaaa 114886 cgggacaaac gctctcctaa gagccgccaa caggtaagtt ttctgcggat ggtcatagcg 114946 gcggcacacg cgtacgagct cggtaaggtg cggcagagct aatgacacac ggccatcacg 115006 catggcgtgc gctacgtgcg ggaggcacgt cggcaccgca gaacgagaca atcctccctc 115066 ggaagaaccc ggaccgcgga gagcgcacag ttcgcagagc cgtttgtcac gtgggctcag 115126 cttggggccg ccatacccat acgaaacgcg cgacaggggc tggaccatga actcctccac 115186 aaggcgctct aactgttcca ctggcatccc gtactcttcg agatacacgg aagctgattt 115246 cggattggca tggaattcgg ccgcggcctc ccgaacctgc gggtcatcga ccaaagactg 115306 tctatagcca cttccatgat atacttgtcc tctactgggt ggggacggga taatagcttc 115366 tggaacaaaa acgcgagcct cggaactatg gaggtcgacg gccaacgctc gtgagagtac 115426 attcagggta atgccgcggc ctaggtctgg gagctccgta ggcgcggtta ctgactgaac 115486 cgacccgtct gtaccgtccg tttcgaccca ctccctcaaa actcgccaga gtggctctag 115546 cgcgacatcg ggacattctc caggtagcac taattgggca gagtcccggt cggggctttc 115606 ggcggatttt tcacttcccg cgctttccag ccttcgggcg agacgtcccc tcctccctga 115666 cagcgagaat gaggaagagg aggaagagga ggaagaggta aggtggctcg ggcccatctt 115726 cttcgaaaat tccctcttcc ctcgcttggg gagacggtct ttgctggtcc tctgcggcga 115786 agggacgggg cgccggttgc tcaaagatcg cgccacggcg cgtcgcgaac gagatctggc 115846 cactctctct gacttcctca ccacttcttg acgcctcaca gcggttgttt tctctactgc 115906 aggtaatgga cacgggcatt caacgtgctg ctgctccatg ggagtttcgc agggatgtgc 115966 gagcgagtcg atgaccgtat aaactccggg ctgatcggta tccacgttaa tatctagtgc 116026 cacgacatca ctggtagcgt tcggacacgg ttctatagag tttatgtttt cgggatccat 116086 gtcgtttaac atcgctataa ggtcgcggag agacatttct cgcccgtcca cgtcttgggc 116146 cgcgtcttgg gccgcattct cagcgatgac agagtgaaag agctcgtcct cgctggaaaa 116206 aagaggtggc gtgctgtacg aaaagctatc agtagtggtc tcacaggtag gtgcgtttgg 116266 agggttcggg atgggagaac ccaaagaatc gtagcgccca ctcttatcgg cgcgcaaaaa 116326 ggtaaataga tctgagggcg ggccagttga gaatcccgac tcatcaacaa ttggagcggc 116386 caaattactc ggcgtcgagg aatcagagga catgatagaa tgctagtgga aggactagcg 116446 atttccagaa tcctgcagaa caagacccga gtgacaaggt tacgtaagtt ttgtgcagaa 116506 gttggaagaa acgtgaagct ttctggtggg gagtagtaac agaaaaaata aagatgcgcg 116566 actctcgaaa cctcccgttt ctcgcgttgg cctgtttatt tccgtccccg caatgcagag 116626 cacaataaag ctccgcaacc ataggcacaa ttttggaaca gcaatacatg ctaaagaagt 116686 ggattatcgt tttgtgcgct tcatgttcat ccataacagc agcccatact cttgactgta 116746 tgggggaatt attcgaagcc cacagtcata tttttgcacg ataccccatg caaaaggata 116806 tttcgtggtc ccctcaaata atgacagccc acctaatccc accccaaaat ttataacaga 116866 atattggttt catgacttac gttttgcgaa gaggtccttt ccacgctggg taggtttcag 116926 caaaggtgtt gtgaagaaag aactttggag agtcaacccc tcggtcttgt gcagttggtt 116986 tgggtatggc atttgtattg aggcatgata taattcagaa ccaatggcgc ccatgcggcc 117046 tgcatatcgt tagcgagcgt atttccagtt ccggtgtgcc atagccaatg catgccatgc 117106 gcccagcata ccattaccgt accaggaact tccggcattt tttatgagcc aacataataa 117166 acaaacctga gcctactgta gaacagatga cgaagataac aaaacaatac attgcttgtg 117226 tccacaggtg cgtgcaattc ggtgccgggg tgtgtgccta ctctagaaag gtcacgcgtg 117286 ttttgttggc attgatatta cccgagaaac acgctcgctt gcacgttatt ggccacaccg 117346 ggaagcagtg aagtttattt tgaatgccaa ggaaagcaca tgggtcacgc ttcctgggag 117406 tgccaaggaa gttgtgacag aaaaaagatc cccatatggg agagttgaca tgtgtaaata 117466 tcaccgtgag cacttgtgtt acttgtgtgt tctaatctct agcaattttc cacgaaagat 117526 tattatcttc tagccacccc tctaacccag cctaaataaa aaattcagac aacgcaaaag 117586 cttggttcca tgttccacgc gagtagttcg cgggaacaca taattacttt attgagacat 117646 ttgccgctcg ggatgcacga atcgcaaatt ttgaatctcg ggatgcgttc tgtatttgca 117706 gggcactttt cttcttttga gagatatggc gagaaggtcc atgctcggga atatagttcg 117766 caatctggtt tgcgatttac aaacggtgaa gaaagaactt tggagagtca acccctcggt 117826 cttgtgcagt tggtttgggt atggcatttg tattgaggca tgatataatt cagatccaat 117886 ggcgcccatg cggcctgcat atcgttagcg agcgtatttc cagttccggt gtgccatagc 117946 caatgcatgc catgcgccca gcataccatt accgtaccag gaacttccgg cattttttat 118006 gagccaacat aataaacaaa cctgagccta ctgtagaaca gatgacgaag ataacaaaac 118066 aatacattgc ttgtgtccac aggtgcgtgc aattcggtgc cggggtgtgt gcctactcta 118126 gaaaggtcac gcgtgttttg ttggcattga tattacccga gaaacacgct cgcttgcacg 118186 ttattggcca caccgggaag cagtgaagtt tattttgaat gccaaggaaa gcacatgggt 118246 cacgcttcct gggagtgcca aggaagttgt gacagaaaaa agatccccat atgggagagt 118306 tgacatgtgt aaatatcacc gtgagcactt gtgttacttg tgtgttctaa tctctagcaa 118366 ttttccacga aagattatta tcttctagcc acccctctaa cccagcctaa ataaaaaatt 118426 cagacaacgc aaaagcttgg ttccatgttc cacgcgagta gttcgcggga acacataatt 118486 actttattga gacatttgcc gctcgggatg cacgaatcgc aaattttgaa tctcgggatg 118546 cgttctgtat ttgcagggca cttttcttct tttgagagat atggcgagaa ggtccatgct 118606 cgggaatata gttcgcaatc tggtttgcga tttacaaacg gtgaagaaag aactttggag 118666 agtcaacccc tcggtcttgt gcagttggtt tgggtatggc atttgtattg aggcatgata 118726 taattcagaa ccaatggcgc ccatgcggcc tgcatatcgt tagcgagcgt atttccagtt 118786 ccggtgtgcc atagccaatg catgccatgc gcccagcata ccattaccgt accaggaact 118846 tccggcattt tttatgagcc aacataataa acaaacctga gcctactgta gaacagatga 118906 cgaagataac aaaacaatac attgcttgtg tccacaggtg cgtgcaattc ggtgccgggg 118966 tgtgtgccta ctctagaaag gtcacgcgtg ttttgttggc attgatatta cccgagaaac 119026 acgctcgctt gcacgttatt ggccacaccg ggaagcagtg aagtttattt tgaatgccaa 119086 ggaaagcaca tgggtcacgc ttcctgggag tgccaaggaa gttgtgacag aaaaaagatc 119146 cccatatggg agagttgaca tgtgtaaata tcaccgtgag cacttgtgtt acttgtgtgt 119206 tctaatctct agcaattttc cacgaaagat tattatcttc tagccacccc tctaacccag 119266 cctaaataaa aaattcagac aacgcaaaag cttggttcca tgttccacgc gagtagttcg 119326 cgggaacaca taattacttt attgagacat ttgccgctcg ggatgcacga atcgcaaatt 119386 ttgaatctcg ggatgcgttc tgtatttgca gggcactttt cttcttttga gagatatggc 119446 gagaaggtcc atgctcggga atatagttcg caatctggtt tgcgatttac aaacggtgaa 119506 gaaagaactt tggagagtca acccctcggt cttgtgcagt tggtttgggt atggcatttg 119566 tattgaggca tgatataatt cagatccaat ggcgcccatg cggcctgcat atcgttagcg 119626 agcgtatttc cagttccggt gtgccatagc caatgcatgc catgcgccca gcataccatt 119686 accgtaccag gaacttccgg cattttttat gagccaacat aataaacaaa cctgagccta 119746 ctgtagaaca gatgacgaag ataacaaaac aatacattgc ttgtgtccac aggtgcgtgc 119806 aattcggtgc cggggtgtgt gcctactcta gaaaggtcac gcgtgttttg ttggcattga 119866 tattacccga gaaacacgct cgcttgcacg ttattggcca caccgggaag cagtgaagtt 119926 tattttgaat gccaaggaaa gcacatgggt cacgcttcct gggagtgcca aggaagttgt 119986 gacagaaaaa agatccccat atgggagagt tgacatgtgt aaatatcacc gtgagcactt 120046 gtgttacttg tgtgttctaa tctctagcaa ttttccacga aagattatta tcttctagcc 120106 acccctctaa cccagcctaa ataaaaaatt cagacaacgc aaaagcttgg ttccatgttc 120166 cacgcgagta gttcgcggga acacataatt actttattga gacatttgcc gctcgggatg 120226 cacgaatcgc aaattttgaa tctcgggatg cgttctgtat ttgcagggca cttttcttct 120286 tttgagagat atggcgagaa ggtccatgct cgggaatata gttcgcaatc tggtttgcga 120346 tttacaaacg ttcctgtaat tttcttacgc ggatctggaa gtaatcgcag gagcctctcc 120406 gcaggagact tgccgattcg ccggtgaatc gcaaatacac ttccagatat atatgtcggg 120466 gggtcaaggc actgtctcta caggtttctt ttaggaaacg cacctatgtc ggattgcccg 120526 tgggactaaa accagttatc tgcgcgtgga agcagccgca ggcttagcag atgcgctcga 120586 aacaaagcac gagttgcaat agccggtata atatcaaggt acgggctata tacatgcgcg 120646 taatacatcc atactggatc tacccagtaa tcgtgttaac tgacggtgtt acaacgcagt 120706 cgtagcagta atatgaaagt atgcaatatg tatgcaataa gcaagggcaa cgaagccgat 120766 aacctgcacg tcggtgggcc caaacaactc agcggcccta ttaaagagca aataaagtag 120826 tgagcccggc acatgccgaa cggctcaagt aaaaacaaaa attcataaag tgcaaacccg 120886 cctatggcgg ttcagtgtta actagctctt cacgacaatc gactttgttg ggtccttatt 120946 ttctgggtca ggtaaactcg aacatgagct ttcgtagagt atttttaacc cgcgatgtct 121006 tggagagtat cggtcacgca gcctccggat gagttcatcc cgccgaatat cacttcccga 121066 tcacattcag ggtcgggggt cgaggggggt gctcgacccg ggtcgtgtgt atcgtgagga 121126 caagcctctc cgatcggcga cccatcccca tctgggacag tgcatggctt taaacggagc 121186 ctgcgtcggc gtggtctgga ggcatcggcg gggggatttt cgggttgctc atgtgagaag 121246 acaatgaccc atcccattgt gcccccagta tgaaatcttc tcccctcttc ctggggtaat 121306 ccgagcagga cccgatggtc ccgctatatg cggggacagc gacctggatc gttcttttat 121366 gcagtgcccg aagatgcaag tcacagctca ttggctggaa gggggatgca ggaccctcgg 121426 tgcgaacgtg acgtaggtgg gtgccaccca catcatgtaa attaggtcac ggggtaggat 121486 tccggctccg cgattggtta tgaggcccct aaagcaaaca ttcgcactcc agtatttaat 121546 atattaggtt ttccctaata tattaaatac tggagtgcga atgtttgctt taggggcctc 121606 ataaccaatc gcggagccgg aatcctaccc cgtgacctaa tttacatgat gtgggtggca 121666 cccacctacg tcacgttcgc accgagggtc caacattttc ctaaaatggc aaagcgggac 121726 tggggtccgt gggtaaattt tctaattttt tcccacggtc tgtcgcaaga gcgtattctc 121786 caagatagaa tgctgttaga ttttcccgaa ttttgtcgag gtcacgcgtt ttgtaatgga 121846 atatggcgcg agaatattga gtttaaattt tgtccgcgcg ccaaacttga agtgatatta 121906 ggacatagtc aaccaaccga catacgttga accatttgcg ggcagcgctg agggaagacg 121966 agatgtatgg acaacacaac gaccggagaa gagggcgtaa agaacatcac ggtcttgctc 122026 tacggtgggg ggtaacaaca atttaataaa taaaaagttt ctttaatata agagcggtat 122086 agcagattta ataaaacacc ttttttgaaa aaatattgac ctgtctcgtt gtagattctt 122146 cgcatccgcg ttccgtcatt attccaagat tttgcgtttc attccaattc aatcaatcct 122206 agaaggcaaa agaaaacata acacgcaaat ataaggatcg gtattttgac attttaaatg 122266 tgtttttaag gaaaagggta gaactttggg gaggagttgg tacgtggtag tcacaatttg 122326 acagacaggt agacagacaa cgaattacaa gcggcctagg gtaatagcgc tgagcccatg 122386 atgtgtccgt gcatatgaga cgactaataa ggtagtcacg taaacccccg tcaatcacac 122446 gcgaaagaaa gaaagaaagg aaaaggcctc aaagcaaatt ttgtagctta aaaaagaggg 122506 ggccctaaag aagaagcaac atcgtctcgg gctgtgtttc cgacgttggg gtgcattatg 122566 cgattttact accagtgttt ggtgtggtgt ggtaagggga aatgagacga gaatttttcg 122626 cgtgcgctcg aggacagagc tacgcccaac cgtgagcaaa ttgcgcggcg cttccttgtt 122686 agcgtggcgt cacgtataaa tgcacagaag cttcgtcagt aacgaagcgc ctctgccaga 122746 gttattaaca ttaggactcg agagagtctc gagaagagac ttctcaaaga gagtgccgct 122806 ttgatctcca gatcacttca cagcccatta agacttttcc tttttgacac acgagtaggg 122866 cgatattttg tgcttatccg agaacggccg caaaccacgg cctactcgaa ccgcgcattc 122926 cctgcggaaa gacaacacga gtaaataaga cccagtgcgc aagatatgag ccacagtcct 122986 ttgtcgagtg atccctctcc cttgccagaa gagacctatc cgtcgccccg cggaaagtca 123046 ccagagcagc ttccctggga tcccgaagag ctctcccaga agtttttctt ttcggacgta 123106 tcggaggacg aagaaccggc acgcgggagg agctggagcg acccggagtc ggaggaagag 123166 cagcctgggt gccggggagt ggacttgggc gaggaggaca cgggacacag ctccaccgag 123226 tcagagccca cgcaatctga cttagacttt attgacgaca gctctccggc gccgccgcca 123286 tttgctatcc cccgcgtccg tgcgttattg cggtgcgcgg caccccgcaa agacccacgg 123346 aaggcttcgg ccgccagggc gggtaggcgc actcttaaaa gacggaggtt gtcattttct 123406 tcttcctctg acgaggaatc cgaggagaga agtaaaaaag aagaagcggc ctcgacccct 123466 gcacggcgac gcaaggccga ggcctcgacg agcagataga ggagacgcgg ggcagaacct 123526 ccccctccct cccacccccc tactctggac atttattgcc cgctcgatcc attctcatcc 123586 agaacttctt tcccgctcag ccttcacgca gaagcggacg cgcgcccctt tgcgaccgcc 123646 ggacatcccg ccgccccccc ccccttcacg cccggcgcaa tccgtagccg tccaactcgg 123706 cccagcacaa ccgcagtaga ccgcccggac cgctctcctc tagacacatc cctaaatgga 123766 aaacatgctc gacgggtgct acccgctggc gctgatggac agcgatcaca ttactgcgca 123826 cgcggtacct cgtggcgagc gcaggcggca aggtgccgct gtcgcctcgt cggagtcggc 123886 cgactcggta gacccgtgca ttcggatcgc ctcgcggctc tggcgcgagt tagtcgagat 123946 atcgtccgaa ctcaaggacg gttacggaga gttcacgtca gcgagagacc gccgcaacgc 124006 gctgattgct gccaacgaac ggctacgttc ggcttttctg ggggccagcc gggcgacgcg 124066 cggcctaggt ttgaggccgc ggtgggcgtc gacggagagc gtcgccaact cccccactga 124126 cccgaataac ggcaacgggt tgggagaatt agaggaggca atggaaggga tcgagggcga 124186 tttctggctc gactctctgg acggtgaccg cttcgaggac gagagccgta ccatgcagag 124246 cgagaatatg cgtttcgtga tcgagaaaga actgttatcc tggctgtccc gacacctgcc 124306 ggccgacctc gcgtccgccg agcgagagac ctcccggtct ctcctggcgg ccgggcactg 124366 gtgctgcttg tggcaccctc ggccgtgccg cgaagcgtgt ttgtacgact cgatttacgt 124426 gcagagtctt ttctgcgtcg ggacggggag agtcccgcaa tcggagatgc gccgtcgcga 124486 atacctggcc gccttgcgcg ccggcgcggc tgccgccaac tctcccgaag tgagcgcctc 124546 gatctttgcg agggacgctg gaatcgcgct ggcgctggcg cggcgccgtt gacgggagaa 124606 tgacgccctc tagcggcttc cttacctccg cgtccctgac aacctcgcgg gtttttacac 124666 tgtcctccgt ccactctccc ccctcaccca ctccgcggca gcgaaacaca accccccccc 124726 tccccagaaa cgagcgacac gcgagcgctg cgaaataaat aaagtaatat tattgtgtgt 124786 ttttcacgtt gttgcaatcg agaggccgtt tgtctgtctg tgtctgtgcg gagctaggct 124846 ttcccgggcg gccccgttcc accgttcggt taggccggtg gcgacgggac atagagaaag 124906 atagagcgcg cgccctggcg gcgagagggt gttgcggggg taaatgggac cctgagctca 124966 ccattttggc gggggattgc acgggtaaca aaaagctctc tcgcacataa tgatttccct 125026 taaacagtgg ctgtaaaagc tttcttcgac tgggacgcgc acgtccggag acatgatctt 125086 atcggtagct acacagttca tgaggtgggc cacgaacgcg cggatcgagt tttgggaacc 125146 ttcggggagg tcttccggga gggtgaagtt tgacagaggc agcgctatca ccaggaggct 125206 ccgcaccatc tccatgccta tccttatcgc cgcgagtccg gcggccggcg cgctgctctg 125266 gttattccag tgcgcggacc gcgagtgcgc ccctccccgg gctctgatat agagcaccgg 125326 cagctcgacg gcggcggaga aaaaagaaag aatgtccggc ccaatgactg gaactttggg 125386 cacgtctctt atttcccacg cggcggcccg gggaatctgc ttgccccaga ccttgctttc 125446 caactccccg ttcggccccc caactaactc cgacagcgcg gtccacagtc ctaccgccgc 125506 tgcgacggcg cgcttagccg cgggcgctat tcgcgggtcg tgcgccgtga tatcttcggc 125566 gacctgcaga ctgcccagcc tttccttccc ttcaaaatac gcgcgggcgg cctgtacgat 125626 caccgcggcc agatcgggcc aaaagaaaat atcgcaactc tgcgacgccc gccagaatct 125686 ccctccgggc aggtccgtgc ccctaaaggc cgccgagaaa gctaagtcca aatgtgacgt 125746 cggaggtctc gacatggtcg ccaaccctcc aaatgctacc cgccggccca cgcaacgcgg 125806 gcttttataa agatggcgcg cgagacaata acacttactc atccgcgtac gcgtttatta 125866 ttgtcaatat ttgtgtggtt attattactg ctaccgccct tgtttctgca aggccctcgc 125926 cgcggcccag gccactattc cggcagcggc cgccgacgcg gcgagcgtcg ccgctaacgt 125986 cggcgccgcg gggagcgggg tttcttcgac ttaaatagac tcccgagaaa aaattttggc 126046 tgccgttcgc catcatccga gtcggaaaca cagtatgcgg ccgagttagg ttttactttt 126106 aaaaacttta ccgtgctgta cggccagggc gttctcaggc tcgaaggggc aagagttgtc 126166 cagactgatg ggtgactcag agacagcgtt gtcttgtctc cgtttaccaa aaatatttcc 126226 actcctctct caaaattttt acctccggtt tcggtaatta ggaaagtttt tggcgcaggg 126286 aggtttaaag ctgccatgca tatgtcagcg gtacccagca cccacaaatg gaactctttt 126346 gcggcatacg cgccagatga caaatggtaa aaccctgcgt ccaagccgct ccactcggga 126406 cttactccag gcgggtcgcc cccctcaccg aaccgaatca cgggtctgca catcctggga 126466 agggaaaaca gctccccgga aacttcgtac agagatgccg ggcgcacgat taccgataat 126526 gtactcggac gatcgtaact cgccatagtt ttcactgcgt gaaccaattc tttccatcca 126586 gaatccgaga gctcaaatct agaattaggt agtttgtagt gcgaatcgac cgcagaaact 126646 atagtcactt ttacaggcgc catcgccgct cagactccac cccgctatga tgtcagaaat 126706 ataacgctct tattctagca gagtcaggcc aatatataca gcttagagaa gatgcggttt 126766 cggcgcatct gttcacgctc tagggcagaa aaacgaagaa gaacaaccga gaatccgctt 126826 acctcaaaac gcgtttgcgt attggatagt ttctcacgga caatgtcatt gcgcccctat 126886 gcagaaattt tgccgaccgc ggaaggcgtc gagcgcctcg ccgaacttgt tagcgtgaca 126946 atgacagaac gcgcggaacc tgtgacagag aatacagctg taaacagtat ccccccggct 127006 aacgagaacg ggcagaactt cgcatatgca ggcgatgggc cctcgactac tgaaaaagtt 127066 gacggctcgc atacagactt cgatgaagca tcgagcgact acgccggccc tgtcccgctc 127126 gcgcaaacta gattgaagca ttcggatgaa tttcttcagc acttccgagt tttagacgat 127186 ttggtggagg gggcttacgg gtttatctgc gacgtccgtc gctacaccga ggaagagcaa 127246 cgtcgaagag gggttaacag tactaaccag gggaaatcaa aatgtaagcg cctgatagct 127306 aaatatgtga aaaatggaac aagggcggcc tctcagctgg aaaatgaaat tttggttctc 127366 gggcgcctaa atcacgagaa tgttctcaag atccaggaaa tccttcggta cccggataat 127426 acgtacatgt taacgcagag gtatcagttc gacttgtaca gctacatgta cgatgaagcg 127486 ttcgactgga aagacagtcc aatgcttaaa cagactagac gcatcatgaa gcagctcatg 127546 tcagcggtct cgtatatcca ttcaaagaaa ctgattcaca gggacatcaa actcgaaaat 127606 attttcttaa actgcgacgg caagacagtg ctgggcgact ttggaactgt cacgcctttt 127666 gaaaatgagc gggagccctt cgaatatgga tgggtgggga ccgtggctac taactctccc 127726 gagatactcg ccagggattc gtactgtgaa attacagaca tttggagctg cggagtagta 127786 ttgctggaaa tggtaagcca tgaattttgc ccgatcggcg atggcggggg aaatccgcac 127846 cagcaattgc tgaaagttat cgactctctc tcagtttgtg atgaagagtt cccagacccc 127906 ccgtgtaatc tgtacaatta tttgcattat gcgagcatcg atcgcgccgg acatacggtc 127966 ccgtcgctca tacggaacct ccaccttccg gcggatgtgg aataccctct agttaaaatg 128026 cttacttttg actggcgttt gagacccagc gcggccgaag tattggcaat gccactgttt 128086 tcggctgaag aggaacggac cataacaatt attcatggaa aacataaacc catccgaccc 128146 gaaatccgtg cgcgggtgcc acggtccatg agtgaaggtt aataataaag gacggagata 128206 gagaactgaa gcgtcagatt tttttaaaaa aataaatgat cgagaactta tgatttgtct 128266 ttcttgaatg accttgcccc atcgattaac gaaaagacct ttcgcgcgtc gattctgctc 128326 ggtctttgtg atacattata gtgagactaa actcgaccga tataacaaga caatgttact 128386 ctatagaccg gactcaacca tgcggcatag cggaggcgac gcaaatcaca gagggataag 128446 gccgaggcgg aaatctattg gagcgtttag cgcgcgcgaa aagactggaa aacgaaatgc 128506 gctgacggaa agcagctcct cctccgacat gctagatccg ttttccacgg ataaggaatt 128566 tggcggtaag tggacggtag acggacctgc cgacattact gccgaggtcc tttctcaggc 128626 atgggacgtt ctccaattag tgaagcatga agatgcggag gaggagagag tgacttatga 128686 gtccaaaccg accccgatac agccgttcaa tgcctggccg gacgggccga gttggaacgc 128746 gcaggatttt actcgagcgc caatagttta tccctctgcg gaggtattgg acgcagaggc 128806 gttgaaagta ggggcattcg ttagccgagt tttacaatgt gtaccgttca cgcgatcaaa 128866 gaaaagcgtt acggtgcggg atgcgcagtc gtttttgggg gactcgttct ggagaataat 128926 gcagaacgtt tacacggttg tcttacgaca gcacataact cgactcaggc acccttccag 128986 caaaagcatt gttaactgca acgaccctct atggtacgcc tacgcgaatc aatttcactg 129046 gagaggaatg cgcgtgccgt cgcttaaatt agcctctccc ccggaggaga atattcaaca 129106 cgacccaatg gccgccgttt ttagaaacgc gggggctggt ctgttcctgt ggcctgccat 129166 gcgcgcagcc tttgaagagc gcgacaagcg actgttaaga gcatgcctgt cttcactcga 129226 tatcatggac gcagccgtcc tcgcgtcgtt tccattttac tggcgcggcg tccaagacac 129286 ctcgcgcttc gagcctgcgc tgggctgttt gtcagagtac tttgcactag tggtgttact 129346 ggccgagacg gtcttagcga ccatgttcga ccacgcactg gtattcatga gggcgctggc 129406 agacggcaat ttcgatgact atgacgaaac tagatatata gaccccgtta aaaacgagta 129466 cctgaacgga gccgagggta ctctgttacg gggcatagtg gcctccaaca ccgctctggc 129526 ggtggtttgc gcaaacacct attcgacgat aagaaaactc ccgtccgtgg caactagcgc 129586 gtgcaatgtt gcctacagga ccgaaacgct gaaagcgagg cgccctggca tgagcgacat 129646 ataccggata ttacaaaaag agtttttctt ttacattgcg tggctccaga gggttgcaac 129706 acacgcaaat ttctgtttaa acattctgaa gagaagcgtg gatacggggg ccccgccatt 129766 tttgttcagg gccagctcgg agaagcggct gcagcagtta aataaaatgc tctgccccct 129826 tctcgtgccg attcaatatg aagacttttc gaaggccatg gggtctgagc tcaagaggga 129886 aaagttagag acattcgtta aagctatttc cagcgacagg gacccgaggg ggtccttaag 129946 atttctcatt tcggaccatg caagggaaat tattgcagac ggagtacggt ttaagccggt 130006 gatagacgag ccggttcggg cttcagttgc gctgagtacc gctgccgctg ggaaagtgaa 130066 agcgcgacgc ttaacctcag ttcgcgcgcc cgtaccgggc gcaggcgccg tttccgcgcg 130126 ccggaaatcg gaaatatgat aaaaatgctt ggcatttgcg ggcgaagagg cgtgatctga 130186 agggctccac aatgacgtaa ctgagctacg catccctata aagtgtaccc gctgaccgct 130246 agcccataca gtgttacagg aggggagaga gacaacttca gctcgaagtc tgaagagaca 130306 tcatgagcgg cttcagtaac ataggatcga ttgccaccgt ttccctagta tgctcgcttt 130366 tgtgcgcatc tgtattaggg gcgccggtac tggacgggct cgagtcgagc cctttcccgt 130426 tcgggggcaa aattatagcc caggcgtgca accgcaccac gattgaggtg acggtcccgt 130486 ggagcgacta ctctggtcgc accgaaggag tgtcagtcga ggtgaaatgg ttctacggga 130546 atagtaatcc cgaaagcttc gtgttcgggg tggatagcga aacgggcagt ggacacgagg 130606 acctgtctac gtgctgggct ctaatccata atctgaacgc gtctgtgtgc agggcgtctg 130666 acgccgggat acctgatttc gacaagcagt gcgaaaaagt gcagagaaga ctgcgctccg 130726 gggtggaact tggtagttac gtgtctggca atggatccct ggtgctgtac ccagggatgt 130786 acgatgccgg catctacgcc taccagctct cagtgggtgg gaagggatat accgggtctg 130846 tttatctaga cgtcggacca aaccccggat gccacgacca gtatgggtac acctattaca 130906 gcctggccga cgaggcgtca gacttatcat cttatgacgt agcctcgccc gaactcgacg 130966 gtcctatgga ggaagattat tccaattgtc tagacatgcc cccgctacgc ccatggacaa 131026 ccgtttgttc gcatgacgtc gaggagcagg aaaacgccac ggacgagctt tacctatggg 131086 acgaggaatg cgccggtccg ctggacgagt acgtcgacga aaggtcagag acgatgccca 131146 ggatggttgt cttttcaccg ccctctacgc tccagcagta gccacccgag agtgtttttt 131206 gtgagcgccc acgcaacata cctaactgct tcatttctga tcaattattg cgtattgaat 131266 aaataaacag tacaaaagca tcaggtgtgg tttgcgtgtc tgtgctaaac catggcgtgt 131326 gcgggtgaaa ccgtaaatta cgtgataata aatagcatag gagttggcgt gcagcgtatt 131386 tcgccgagag atggggacaa tgttagtgtt gcgccttttc ctacttgcag tagcggacgc 131446 ggcgttgccg accggcagat tctgccgagt ttggaaggtg cctccgggag gaaccatcca 131506 agagaacctg gcggtgctcg cggaatcgcc ggtcacggga cacgcgacat atccgccgcc 131566 tgaaggcgcc gtcagctttc agatttttgc ggacacccct actttgcgca ttcgctacgg 131626 cgctacggag gacgaacttg cactggagcg cgggacgtcc gcctcagacg cggacaacgt 131686 gacattttcg ctgtcatatc gcccgcgccc agaaattcac ggagcatact tcaccatagg 131746 ggtattcgct actggccaga gcacggaaag cagctattcg gtcatcagtc gggtcttagt 131806 taacgcctct ctggaacggt ccgtgcgcct ggaaacgccg tgcgatgaaa attttttgca 131866 gaacgagcct acatggggct cgaagcgttg gttaggcccc ccgtcgcctt atgtgcgaga 131926 taacgatgtc gccgtgttga caaaagcgca gtacattggg gagtgctact ccaactcggc 131986 ggcccagacg gggctcacgt ctctcaacat gacctttttc tattcgccta aaagaatagt 132046 aaacgtcacg tggacaaccg gcggcccctc cccctcgcgc ataacggtat actcgtcgcg 132106 ggagaacggg cagcccgtgt tgaggaacgt ttctgacggg ttcttggtta agtacactcc 132166 cgacattgac ggccgggcca tgataaacgt tattgccaat tattcgccgg cggactccgg 132226 cagcgtcctc gcgtttacgg cctttaggga aggaaaactc ccatccgcga ttcaactgca 132286 ccggatagat atgtccggga ctgagccgcc ggggactgaa acgaccttcg actgtcaaaa 132346 aatgatagaa accccgtacc gagcgctcgg gagcaatgtt cccagggacg actctatccg 132406 tccgggggcc actctgcctc cgttcgatac cgcagcacct gatttcgata caggtacttc 132466 cccgaccccc actaccgtgc cagagccagc cattactaca ctcataccgc gcagcactag 132526 cgatatggga ttcttctcca cggcacgtgc taccggatca gaaactcttt cggtacccgt 132586 ccaggaaacg gatagaactc tttcgacaac tcctcttacc cttccactga ctcccggtga 132646 gtcagaaaat acactgtttc ctacgaccgc gccggggatt tctaccgaga ccccgagcgc 132706 ggcacatgaa actacacaga cccagagtgc agaaacggtg gtctttactc agagtccgag 132766 taccgagtcg gaaaccgcgc ggtcccagag tcaggaaccg tggtatttta ctcagactcc 132826 gagtactgaa caggcggctc ttactcagac gcagatcgca gaaacggagg cgttgtttac 132886 tcagactccg agtgctgaac agatgacttt tactcagact ccgggtgcag aaaccgaggc 132946 acctgcccag accccgagca cgatacccga gatatttact cagtctcgta gcacgccccc 133006 cgaaaccgct cgcgctccga gcgcggcgcc ggaggttttt acacagagtt cgagtacggt 133066 aacggaggtg tttactcaga ccccgagcac ggtaccgaaa actactctga gttcgagtac 133126 tgaaccggcg atttttactc ggactcagag cgcgggaact gaggccttta ctcagacttc 133186 gagtgccgag ccggacacta tgcgaactca gagtactgaa acacactttt tcactcaggc 133246 cccgagtacg gtaccgaaag ctactcagac tccgagtaca gagccggagg tgttgactca 133306 gagtccgagt accgaacctg tgcctttcac ccggactctg ggcgcagagc cggaaattac 133366 tcagaccccg agcgcggcac cggaggttta tactcggagt tcgagtacga tgccagaaac 133426 tgcacagagc acacccctgg cctcgcaaaa ccctaccagt tcgggaaccg ggacgcataa 133486 tactgaaccg aggacttatc cagtgcaaac gacaccacat acccagaaac tctacacaga 133546 aaataagact ttatcgtttc ctactgttgt ttcagaattc catgagatgt cgacggcaga 133606 gtcgcagacg cccctattgg acgtcaaaat tgtagaggtg aagttttcaa acgatggcga 133666 agtaacggcg acttgcgttt ccaccgtcaa atctccctat agggtagaaa ctaattggaa 133726 agtagacctc gtagatgtaa tggatgaaat ttctgggaac agtcccgccg gggtttttaa 133786 cagtaatgag aaatggcaga aacagctgta ctacagagta accgatggaa gaacatcggt 133846 ccagctaatg tgcctgtcgt gcacgagcca ttctccggaa ccttactgtc ttttcgacac 133906 gtctcttata gcgagggaaa aagatatcgc gccagagtta tactttacct ctgatccgca 133966 aacggcatac tgcacaataa ctctgccgtc cggcgttgtt ccgagattcg aatggagcct 134026 taataatgtt tcactgccgg aatatttgac ggccacgacc gttgtttcgc ataccgctgg 134086 ccaaagtaca gtgtggaaga gcagcgcgag agcaggcgag gcgtggattt ctggccgggg 134146 aggcaatata tacgaatgca ccgtcctcat ctcagacggc actcgcgtta ctacgcgaaa 134206 ggagaggtgc ttaacaaaca catggattgc ggtggaaaac ggtgctgctc aggcgcagct 134266 gtattcactc ttttctggac ttgtgtcagg attatgcggg agcatatctg ctttgtacgc 134326 aacgctatgg accgccattt atttttgagg aatgcttttt ggactatcgt actgctttct 134386 tccttcgcta gccagagcac cgccgccgtc acgtacgact acattttagg ccgtcgcgcg 134446 ctcgacgcgc taaccatacc ggcggttggc ccgtataaca gatacctcac tagggtatca 134506 agaggctgcg acgttgtcga gctcaacccg atttctaacg tggacgacat gatatcggcg 134566 gccaaagaaa aagagaaggg gggccctttc gaggcctccg tcgtctggtt ctacgtgatt 134626 aagggcgacg acggcgagga caagtactgt ccaatctata gaaaagagta cagggaatgt 134686 ggcgacgtac aactgctatc tgaatgcgcc gttcaatctg cacagatgtg ggcagtggac 134746 tatgttccta gcacccttgt atcgcgaaat ggcgcgggac tgactatatt ctcccccact 134806 gctgcgctct ctggccaata cttgctgacc ctgaaaatcg ggagatttgc gcaaacagct 134866 ctcgtaactc tagaagttaa cgatcgctgt ttaaagatcg ggtcgcagct taacttttta 134926 ccgtcgaaat gctggacaac agaacagtat cagactggat ttcaaggcga acacctttat 134986 ccgatcgcag acaccaatac acgacacgcg gacgacgtat atcggggata cgaagatatt 135046 ctgcagcgct ggaataattt gctgaggaaa aagaatccta gcgcgccaga ccctcgtcca 135106 gatagcgtcc cgcaagaaat tcccgctgta accaagaaag cggaagggcg caccccggac 135166 gcagaaagca gcgaaaagaa ggcccctcca gaagactcgg aggacgacat gcaggcagag 135226 gcttctggag aaaatcctgc cgccctcccc gaagacgacg aagtccccga ggacaccgag 135286 cacgatgatc caaactcgga tcctgactat tacaatgaca tgcccgccgt gatcccggtg 135346 gaggagacta ctaaaagttc taatgccgtc tccatgccca tattcgcggc gttcgtagcc 135406 tgcgcggtcg cgctcgtggg gctactggtt tggagcatcg taaaatgcgc gcgtagctaa 135466 tcgagcctag aataggtggt ttcttcctac atgccacgcc tcacgctcat aatataaatc 135526 acatggaata gcataccaat gcctattcat tgggacgttc gaaaagcatg gcatcgctac 135586 ttggaactct ggctctcctt gccgcgacgc tcgcaccctt cggcgcgatg ggaatcgtga 135646 tcactggaaa tcacgtctcc gccaggattg acgacgatca catcgtgatc gtcgcgcctc 135706 gccccgaagc tacaattcaa ctgcagctat ttttcatgcc tggccagaga ccccacaaac 135766 cctactcagg aaccgtccgc gtcgcgtttc ggtctgatat aacaaaccag tgctaccagg 135826 aacttagcga ggagcgcttt gaaaattgca ctcatcgatc gtcttctgtt tttgtcggct 135886 gtaaagtgac cgagtacacg ttctccgcct cgaacagact aaccggacct ccacacccgt 135946 ttaagctcac tatacgaaat cctcgtccga acgacagcgg gatgttctac gtaattgttc 136006 ggctagacga caccaaagaa cccattgacg tcttcgcgat ccaactatcg gtgtatcaat 136066 tcgcgaacac cgccgcgact cgcggactct attccaaggc ttcgtgtcgc accttcggat 136126 tacctaccgt ccaacttgag gcctatctca ggaccgagga aagttggcgc aactggcaag 136186 cgtacgttgc cacggaggcc acgacgacca gcgccgaggc gacaaccccg acgcccgtca 136246 ctgcaaccag cgcctccgaa cttgaagcgg aacactttac ctttccctgg ctagaaaatg 136306 gcgtggatca ttacgaaccg acacccgcaa acgaaaattc aaacgttact gtccgtctcg 136366 ggacaatgag ccctacgcta attggggtaa ccgtggctgc cgtcgtgagc gcaacgatcg 136426 gcctcgtcat tgtaatttcc atcgtcacca gaaacatgtg caccccgcac cgaaaattag 136486 acacggtctc gcaagacgac gaagaacgtt cccaaactag aagggaatcg cgaaaatttg 136546 gacccatggt tgcgtgcgaa ataaacaagg gggctgacca ggatagtgaa cttgtggaac 136606 tggttgcgat tgttaacccg tctgcgctaa gctcgcccga ctcaataaaa atgtgattaa 136666 gtctgaatgt ggctctccaa tcatttcgat tctctaatct cccaatcctc tcaaaagggg 136726 cagtatcgga cacggactgg gaggggcgta cacgatagtt atatggtaca gcagaggcct 136786 ctgaacactt aggaggagaa ttcagccggg gagagcccct gttgagtagg cttgggagca 136846 tattgcagga tgaacatgtt agtgatagtt ctcgcctctt gtcttgcgcg cctaactttt 136906 gcgacgcgac acgtcctctt tttggaaggc actcaggctg tcctcgggga agatgatccc 136966 agaaacgttc cggaagggac tgtaatcaaa tggacaaaag tcctgcggaa cgcgtgcaag 137026 atgaaggcgg ccgatgtctg ctcttcgcct aactattgct ttcatgattt aatttacgac 137086 ggaggaaaga aagactgccc gcccgcggga cccctgtctg caaacctggt aattttacta 137146 aagcgcggcg aaagcttcgt cgtgctgggt tctgggctac acaacagcaa tataactaat 137206 atcatgtgga cagagtacgg aggcctgctc tttgatcctg taactcgttc ggacgaggga 137266 atctattttc gacggatctc tcagccagat ctggccatgg aaactacatc gtacaacgtc 137326 agcgttcttt cgcacgtaga cgagaaggct ccagcaccgc acgaggtgga gatagacacc 137386 atcaagccgt cagaggccca cgcgcacgtg gaattacaaa tgctgccgtt tcatgaactc 137446 aacgacaaca gccccaccta tgtgacccct gttcttagag tcttcccacc gaccgagcac 137506 gtaaaattta acgttacgta ttcgtggtat gggtttgatg tcaaagagga gtgcgaagaa 137566 gtgaaactgt tcgagccgtg cgtataccat cctacagacg gcaaatgtca gtttcccgca 137626 accaaccaga gatgcctcat aggatctgtc ttgatggcgg aattcttggg cgcggcctct 137686 ttgctggatt gttcccgcga tactctagaa gactgccacg aaaatcgcgt gccgaaccta 137746 cggttcgatt cgcgactctc cgagtcacgc gcaggcctgg tgatcagtcc tcttatagcc 137806 atccccaaag ttttgattat agtcgtttcc gacggagaca ttttgggatg gagctacacg 137866 gtgctcggga aacgtaacag tccgcgcgta gtagtcgaaa cgcacatgcc ctcgaaggtc 137926 ccgatgaaca aagtagtaat tggcagtccc ggaccaatgg acgaaacggg taactataaa 137986 atgtacttcg tcgtcgcggg ggtggccgcg acgtgcgtaa ttcttacatg cgctctgctt 138046 gtggggaaaa agaagtgccc cgcgcaccaa atgggtactt tttccaagac cgaaccattg 138106 tacgcgccgc tccccaaaaa cgagtttgag gccggcgggc ttacggacga tgaggaagtg 138166 atttatgacg aagtatacga acccctattt cgcggctact gtaagcagga attccgcgaa 138226 gatgtgaata cctttttcgg tgcggtcgtg gagggagaaa gggccttaaa ctttaaatcc 138286 gccatcgcat caatggcaga tcgcatcctg gcaaataaaa gcggcagaag gaatatggat 138346 agctattagt tggtcatgcc ttttaagacc agaggggccg aagacgcggc cgcgggcaag 138406 aacaggttta agaaatcgag aaatcgggaa atcttaccga ccagactgcg tggcaccggt 138466 aagaaaactg ccggattgtc caattatacc cagcctattc cctggaaccc taaattctgc 138526 agcgcgcgcg gggaatctga caaccacgcg tgtaaagaca ctttttatcg caggacgtgc 138586 tgcgcatcgc gctctaccgt ttccagtcaa cccgattccc cccacacacc catgcctact 138646 gagtatgggc gcgtgccctc cgcaaagcgc aaaaaactat catcttcaga ctgcgagggc 138706 gcgcaccaac ccctagtatc ctgtaaactt ccggattctc aagcagcacc ggcgcgaacc 138766 tatagttctg cgcaaagata tactgttgac gaggtttcgt cgccaactcc gccaggcgtc 138826 gacgctgttg cggacttaga aacgcgcgcg gaacttcctg gcgctacgac ggaacaaacg 138886 gaaagtaaaa ataagctccc caaccaacaa tcgcgcctga agccgaaacc cacaaacgag 138946 cacgtcggag gggagcggtg cccctccgaa ggcacggtcg aggcgccatc gctcggcatc 139006 ctctcgcgcg tcggggcagc gatagcaaac gagctggctc gtatgcggag ggcgtgtctt 139066 ccgctcgccg cgtcggcggc cgctgccgga atagtggcct gggccgcggc gagggccttg 139126 cagaaacaag ggcggtagca gtaataataa ccacacaaat attgacaata ataaacgcgt 139186 acgcggatga gtaagtgtta ttgtctcgcg cgccatcttt ataaaagccc gcgttgcgtg 139246 ggccggcggg tagcatttgg agggttggcg accatgtcga gacctccgac gtcacatttg 139306 gacttagctt tctcggcggc ctttaggggc acggacctgc ccggagggag attctggcgg 139366 gcgtcgcaga gttgcgatat tttcttttgg cccgatctgg ccgcggtgat cgtacaggcc 139426 gcccgcgcgt attttgaagg gaaggaaagg ctgggcagtc tgcaggtcgc cgaagatatc 139486 acggcgcacg acccgcgaat agcgcccgcg gctaagcgcg ccgtcgcagc ggcggtagga 139546 ctgtggaccg cgctgtcgga gttagttggg gggccgaacg gggagttgga aagcaaggtc 139606 tggggcaagc agattccccg ggccgccgcg tgggaaataa gagacgtgcc caaagttcca 139666 gtcattgggc cggacattct ttcttttttc tccgccgccg tcgagctgcc ggtgctctat 139726 atcagagccc ggggaggggc gcactcgcgg tccgcgcact ggaataacca gagcagcgcg 139786 ccggccgccg gactcgcggc gataaggata ggcatggaga tggtgcggag cctcctggtg 139846 atagcgctgc ctctgtcaaa cttcaccctc ccggaagacc tccccgaagg ttcccaaaac 139906 tcgatccgcg cgttcgtggc ccacctcatg aactgtgtag ctaccgataa gatcatgtct 139966 ccggacgtgc gcgtcccagt cgaagaaagc ttttacagcc actgtttaag ggaaatcatt 140026 atgtgcgaga gagctttttg ttacccgtgc aatcccccgc caaaatggtg agctcagggt 140086 cccatttacc cccgcaacac cctctcgccg ccagggcgcg cgctctatct ttctctatgt 140146 cccgtcgcca ccggcctaac cgaacggtgg aacggggccg cccgggaaag cctagctccg 140206 cacagacaca gacagacaaa cggcctctcg attgcaacaa cgtgaaaaac acacaataat 140266 attactttat ttatttcgca gcgctcgcgt gtcgctcgtt tctggggagg gggggggttg 140326 tgtttcgctg ccgcggagtg ggtgaggggg gagagtggac ggaggacagt gtaaaaaccc 140386 gcgaggttgt cagggacgcg gaggtaagga agccgctaga gggcgtcatt ctcccgtcaa 140446 cggcgccgcg ccagcgccag cgcgattcca gcgtccctcg caaagatcga ggcgctcact 140506 tcgggagagt tggcggcagc cgcgccggcg cgcaaggcgg ccaggtattc gcgacggcgc 140566 atctccgatt gcgggactct ccccgtcccg acgcagaaaa gactctgcac gtaaatcgag 140626 tcgtacaaac acgcttcgcg gcacggccga gggtgccaca agcagcacca gtgcccggcc 140686 gccaggagag accgggaggt ctctcgctcg gcggacgcga ggtcggccgg caggtgtcgg 140746 gacagccagg ataacagttc tttctcgatc acgaaacgca tattctcgct ctgcatggta 140806 cggctctcgt cctcgaagcg gtcaccgtcc agagagtcga gccagaaatc gccctcgatc 140866 ccttccattg cctcctctaa ttctcccaac ccgttgccgt tattcgggtc agtgggggag 140926 ttggcgacgc tctccgtcga cgcccaccgc ggcctcaaac ctaggccgcg cgtcgcccgg 140986 ctggccccca gaaaagccga acgtagccgt tcgttggcag caatcagcgc gttgcggcgg 141046 tctctcgctg acgtgaactc tccgtaaccg tccttgagtt cggacgatat ctcgactaac 141106 tcgcgccaga gccgcgaggc gatccgaatg cacgggtcta ccgagtcggc cgactccgac 141166 gaggcgacag cggcaccttg ccgcctgcgc tcgccacgag gtaccgcgtg cgcagtaatg 141226 tgatcgctgt ccatcagcgc cagcgggtag cacccgtcga gcatgttttc catttaggga 141286 tgtgtctaga ggagagcggt ccgggcggtc tactgcggtt gtgctgggcc gagttggacg 141346 gctacggatt gcgccgggcg tgaagggggg gggggcggcg ggatgtccgg cggtcgcaaa 141406 ggggcgcgcg tccgcttctg cgtgaaggct gagcgggaaa gaagttctgg atgagaatgg 141466 atcgagcggg caataaatgt ccagagtagg ggggtgggag ggagggggag gttctgcccc 141526 gcgtctcctc tatctgctcg tcgaggcctc ggccttgcgt cgccgtgcag gggtcgaggc 141586 cgcttcttct tttttacttc tctcctcgga ttcctcgtca gaggaagaag aaaatgacaa 141646 cctccgtctt ttaagagtgc gcctacccgc cctggcggcc gaagccttcc gtgggtcttt 141706 gcggggtgcc gcgcaccgca ataacgcacg gacgcggggg atagcaaatg gcggcggcgc 141766 cggagagctg tcgtcaataa agtctaagtc agattgcgtg ggctctgact cggtggagct 141826 gtgtcccgtg tcctcctcgc ccaagtccac tccccggcac ccaggctgct cttcctccga 141886 ctccgggtcg ctccagctcc tcccgcgtgc cggttcttcg tcctccgata cgtccgaaaa 141946 gaaaaacttc tgggagagct cttcgggatc ccagggaagc tgctctggtg actttccgcg 142006 gggcgacgga taggtctctt ctggcaaggg agagggatca ctcgacaaag gactgtggct 142066 catatcttgc gcactgggtc ttatttactc gtgttgtctt tccgcaggga atgcgcggtt 142126 cgagtaggcc gtggtttgcg gccgttctcg gataagcaca aaatatcgcc ctactcgtgt 142186 gtcaaaaagg aaaagtctta atgggctgtg aagtgatctg gagatcaaag cggcactctc 142246 tttgagaagt ctcttctcga gactctctcg agtcctaatg ttaataactc tggcagaggc 142306 gcttcgttac tgacgaagct tctgtgcatt tatacgtgac gccacgctaa caaggaagcg 142366 ccgcgcaatt tgctcacggt tgggcgtagc tctgtcctcg agcgcacgcg aaaaattctc 142426 gtctcatttc cccttaccac accacaccaa acactggtag taaaatcgca taatgcaccc 142486 caacgtcgga aacacagccc gagacgatgt tgcttcttct ttagggcccc ctctttttta 142546 agctacaaaa tttgctttga ggccttttcc tttctttctt tctttcgcgt gtgattgacg 142606 ggggtttacg tgactacctt attagtcgtc tcatatgcac ggacacatca tgggctcagc 142666 gctattaccc taggccgctt gtaattcgtt gtctgtctac ctgtctgtca aattgtgact 142726 accacgtacc aactcctccc caaagttcta cccttttcct taaaaacaca tttaaaatgt 142786 caaaataccg atccttatat ttgcgtgtta tgttttcttt tgccttctag gattgattga 142846 attggaatga aacgcaaaat cttggaataa tgacggaacg cggatgcgaa gaatctacaa 142906 cgagacaggt caatattttt tcaaaaaagg tgttttatta aatctgctat accgctctta 142966 tattaaagaa actttttatt tattaaattg ttgttacccc ccaccgtaga gcaagaccgt 143026 gatgttcttt acgccctctt ctccggtcgt tgtgttgtcc atacatctcg tcttccctca 143086 gcgctgcccg caaatggttc aacgtatgtc ggttggttga ctatgtccta atatcacttc 143146 aagtttggcg cgcggacaaa atttaaactc aatattctcg cgccatattc cattacaaaa 143206 cgcgtgacct cgacaaaatt cgggaaaatc taacagcatt ctatcttgga gaatacgctc 143266 ttgcgacaga ccgtgggaaa aaattagaaa atttacccac ggaccccagt cccgctttgc 143326 cattttagga aaatgttgga ccctcggtgc gaacgtgacg taggtgggtg ccacccacat 143386 catgtaaatt aggtcacggg gtaggattcc ggctccgcga ttggttatga ggcccctaaa 143446 gcaaacattc gcactccagt atttaatata ttagggaaaa cctaatatat taaatactgg 143506 agtgcgaatg tttgctttag gggcctcata accaatcgcg gagccggaat cctaccccgt 143566 gacctaattt acatgatgtg ggtggcaccc acctacgtca cgttcgcacc gagggtcctg 143626 catccccctt ccagccaatg agctgtgact tgcatcttcg ggcactgcat aaaagaacga 143686 tccaggtcgc tgtccccgca tatagcggga ccatcgggtc ctgctcggat taccccagga 143746 agaggggaga agatttcata ctgggggcac aatgggatgg gtcattgtct tctcacatga 143806 gcaacccgaa aatccccccg ccgatgcctc cagaccacgc cgacgcaggc tccgtttaaa 143866 gccatgcact gtcccagatg gggatgggtc gccgatcgga gaggcttgtc ctcacgatac 143926 acacgacccg ggtcgagcac ccccctcgac ccccgaccct gaatgtgatc gggaagtgat 143986 attcggcggg atgaactcat ccggaggctg cgtgaccgat actctccaag acatcgcggg 144046 ttaaaaatac tctacgaaag ctcatgttcg agtttacctg acccagaaaa taaggaccca 144106 acaaagtcga ttgtcgtgaa gagctagtta acactgaacc gccataggcg ggtttgcact 144166 ttatgaattt ttgtttttac ttgagccgtt cggcatgtgc cgggctcact actttatttg 144226 ctctttaata gggccgctga gttgtttggg cccaccgacg tgcaggttat cggcttcgtt 144286 gcccttgctt attgcataca tattgcatac tttcatatta ctgctacgac tgcgttgtaa 144346 caccgtcagt taacacgatt actgggtaga tccagtatgg atgtattacg cgcatgtata 144406 tagcccgtac cttgatatta taccggctat tgcaactcgt gctttgtttc gagcgcatct 144466 gctaagcctg cggctgcttc cacgcgcaga taactggttt tagtcccacg ggcaatccga 144526 cataggtgcg tttcctaaaa gaaacctgta gagacagtgc cttgaccccc cgacatatat 144586 atctggaagt gtatttgcga ttcaccggcg aatcggcaag tctcctgcgg agaggctcct 144646 gcgattactt ccagatccgc gtaagaaaat tacaggaacg tttgtaaatc gcaaaccaga 144706 ttgcgaacta tattcccgag catggacctt ctcgccatat ctctcaaaag aagaaaagtg 144766 ccctgcaaat acagaacgca tcccgagatt caaaatttgc gattcgtgca tcccgagcgg 144826 caaatgtctc aataaagtaa ttatgtgttc ccgcgaacta ctcgcgtgga acatggaacc 144886 aagcttttgc gttgtctgaa ttttttattt aggctgggtt agaggggtgg ctagaagata 144946 ataatctttc gtggaaaatt gctagagatt agaacacaca agtaacacaa gtgctcacgg 145006 tgatatttac acatgtcaac tctcccatat ggggatcttt tttctgtcac aacttccttg 145066 gcactcccag gaagcgtgac ccatgtgctt tccttggcat tcaaaataaa cttcactgct 145126 tcccggtgtg gccaataacg tgcaagcgag cgtgtttctc gggtaatatc aatgccaaca 145186 aaacacgcgt gacctttcta gagtaggcac acaccccggc accgaattgc acgcacctgt 145246 ggacacaagc aatgtattgt tttgttatct tcgtcatctg ttctacagta ggctcaggtt 145306 tgtttattat gttggctcat aaaaaatgcc ggaagttcct ggtacggtaa tggtatgctg 145366 ggcgcatggc atgcattggc tatggcacac cggaactgga aatacgctcg ctaacgatat 145426 gcaggccgca tgggcgccat tggatctgaa ttatatcatg cctcaataca aatgccatac 145486 ccaaaccaac tgcacaagac cgaggggttg actctccaaa gttctttctt caccgtttgt 145546 aaatcgcaaa ccagattgcg aactatattc ccgagcatgg accttctcgc catatctctc 145606 aaaagaagaa aagtgccctg caaatacaga acgcatcccg agattcaaaa tttgcgattc 145666 gtgcatcccg agcggcaaat gtctcaataa agtaattatg tgttcccgcg aactactcgc 145726 gtggaacatg gaaccaagct tttgcgttgt ctgaattttt tatttaggct gggttagagg 145786 ggtggctaga agataataat ctttcgtgga aaattgctag agattagaac acacaagtaa 145846 cacaagtgct cacggtgata tttacacatg tcaactctcc catatgggga tcttttttct 145906 gtcacaactt ccttggcact cccaggaagc gtgacccatg tgctttcctt ggcattcaaa 145966 ataaacttca ctgcttcccg gtgtggccaa taacgtgcaa gcgagcgtgt ttctcgggta 146026 atatcaatgc caacaaaaca cgcgtgacct ttctagagta ggcacacacc ccggcaccga 146086 attgcacgca cctgtggaca caagcaatgt attgttttgt tatcttcgtc atctgttcta 146146 cagtaggctc aggtttgttt attatgttgg ctcataaaaa atgccggaag ttcctggtac 146206 ggtaatggta tgctgggcgc atggcatgca ttggctatgg cacaccggaa ctggaaatac 146266 gctcgctaac gatatgcagg ccgcatgggc gccattggtt ctgaattata tcatgcctca 146326 atacaaatgc catacccaaa ccaactgcac aagaccgagg ggttgactct ccaaagttct 146386 ttcttcaccg tttgtaaatc gcaaaccaga ttgcgaacta tattcccgag catggacctt 146446 ctcgccatat ctctcaaaag aagaaaagtg ccctgcaaat acagaacgca tcccgagatt 146506 caaaatttgc gattcgtgca tcccgagcgg caaatgtctc aataaagtaa ttatgtgttc 146566 ccgcgaacta ctcgcgtgga acatggaacc aagcttttgc gttgtctgaa ttttttattt 146626 aggctgggtt agaggggtgg ctagaagata ataatctttc gtggaaaatt gctagagatt 146686 agaacacaca agtaacacaa gtgctcacgg tgatatttac acatgtcaac tctcccatat 146746 ggggatcttt tttctgtcac aacttccttg gcactcccag gaagcgtgac ccatgtgctt 146806 tccttggcat tcaaaataaa cttcactgct tcccggtgtg gccaataacg tgcaagcgag 146866 cgtgtttctc gggtaatatc aatgccaaca aaacacgcgt gacctttcta gagtaggcac 146926 acaccccggc accgaattgc acgcacctgt ggacacaagc aatgtattgt tttgttatct 146986 tcgtcatctg ttctacagta ggctcaggtt tgtttattat gttggctcat aaaaaatgcc 147046 ggaagttcct ggtacggtaa tggtatgctg ggcgcatggc atgcattggc tatggcacac 147106 cggaactgga aatacgctcg ctaacgatat gcaggccgca tgggcgccat tggatctgaa 147166 ttatatcatg cctcaataca aatgccatac ccaaaccaac tgcacaagac cgaggggttg 147226 actctccaaa gttctttctt caccgtttgt aaatcgcaaa ccagattgcg aactatattc 147286 ccgagcatgg accttctcgc catatctctc aaaagaagaa aagtgccctg caaatacaga 147346 acgcatcccg agattcaaaa tttgcgattc gtgcatcccg agcggcaaat gtctcaataa 147406 agtaattatg tgttcccgcg aactactcgc gtggaacatg gaaccaagct tttgcgttgt 147466 ctgaattttt tatttaggct gggttagagg ggtggctaga agataataat ctttcgtgga 147526 aaattgctag agattagaac acacaagtaa cacaagtgct cacggtgata tttacacatg 147586 tcaactctcc catatgggga tcttttttct gtcacaactt ccttggcact cccaggaagc 147646 gtgacccatg tgctttcctt ggcattcaaa ataaacttca ctgcttcccg gtgtggccaa 147706 taacgtgcaa gcgagcgtgt ttctcgggta atatcaatgc caacaaaaca cgcgtgacct 147766 ttctagagta ggcacacacc ccggcaccga attgcacgca cctgtggaca caagcaatgt 147826 attgttttgt tatcttcgtc atctgttcta cagtaggctc aggtttgttt attatgttgg 147886 ctcataaaaa atgccggaag ttcctggtac ggtaatggta tgctgggcgc atggcatgca 147946 ttggctatgg cacaccggaa ctggaaatac gctcgctaac gatatgcagg ccgcatgggc 148006 gccattggtt ctgaattata tcatgcctca atacaaatgc catacccaaa ccaactgcac 148066 aagaccgagg ggttgactct ccaaagttct ttcttcacaa cacctttgct gaaacctacc 148126 cagcgtggaa aggacctctt cgcaaaacgt aagtcatgaa accaatattc tgttataaat 148186 tttggggtgg gattaggtgg gctgtcatta tttgagggga ccacgaaata tccttttgca 148246 tggggtatcg tgcaaaaata tgactgtggg cttcgaataa ttcccccata cagtcaagag 148306 tatgggctgc tgttatggat gaacatgaag cgcacaaaac gataatccac ttctttagca 148366 tgtattgctg ttccaaaatt gtgcctatgg ttgcggagct ttattgtgct ctgcattgcg 148426 gggacggaaa taaacaggcc aacgcgagaa acgggaggtt tcgagagtcg cgcatcttta 148486 ttttttctgt tactactccc caccagaaag cttcacgttt cttccaactt ctgcacaaaa 148546 cttacgtaac cttgtcactc gggtcttgtt ctgcaggatt ctggaaatcg ctagtccttc 148606 cactagcatt ctatcatgtc ctctgattcc tcgacgccga gtaatttggc cgctccaatt 148666 gttgatgagt cgggattctc aactggcccg ccctcagatc tatttacctt tttgcgcgcc 148726 gataagagtg ggcgctacga ttctttgggt tctcccatcc cgaaccctcc aaacgcacct 148786 acctgtgaga ccactactga tagcttttcg tacagcacgc cacctctttt ttccagcgag 148846 gacgagctct ttcactctgt catcgctgag aatgcggccc aagacgcggc ccaagacgtg 148906 gacgggcgag aaatgtctct ccgcgacctt atagcgatgt taaacgacat ggatcccgaa 148966 aacataaact ctatagaacc gtgtccgaac gctaccagtg atgtcgtggc actagatatt 149026 aacgtggata ccgatcagcc cggagtttat acggtcatcg actcgctcgc acatccctgc 149086 gaaactccca tggagcagca gcacgttgaa tgcccgtgtc cattacctgc agtagagaaa 149146 acaaccgctg tgaggcgtca agaagtggtg aggaagtcag agagagtggc cagatctcgt 149206 tcgcgacgcg ccgtggcgcg atctttgagc aaccggcgcc ccgtcccttc gccgcagagg 149266 accagcaaag accgtctccc caagcgaggg aagagggaat tttcgaagaa gatgggcccg 149326 agccacctta cctcttcctc ctcttcctcc tcttcctcat tctcgctgtc agggaggagg 149386 ggacgtctcg cccgaaggct ggaaagcgcg ggaagtgaaa aatccgccga aagccccgac 149446 cgggactctg cccaattagt gctacctgga gaatgtcccg atgtcgcgct agagccactc 149506 tggcgagttt tgagggagtg ggtcgaaacg gacggtacag acgggtcggt tcagtcagta 149566 accgcgccta cggagctccc agacctaggc cgcggcatta ccctgaatgt actctcacga 149626 gcgttggccg tcgacctcca tagttccgag gctcgcgttt ttgttccaga agctattatc 149686 ccgtccccac ccagtagagg acaagtatat catggaagtg gctatagaca gtctttggtc 149746 gatgacccgc aggttcggga ggccgcggcc gaattccatg ccaatccgaa atcagcttcc 149806 gtgtatctcg aagagtacgg gatgccagtg gaacagttag agcgccttgt ggaggagttc 149866 atggtccagc ccctgtcgcg cgtttcgtat gggtatggcg gccccaagct gagcccacgt 149926 gacaaacggc tctgcgaact gtgcgctctc cgcggtccgg gttcttccga gggaggattg 149986 tctcgttctg cggtgccgac gtgcctcccg cacgtagcgc acgccatgcg tgatggccgt 150046 gtgtcattag ctctgccgca ccttaccgag ctcgtacgcg tgtgccgccg ctatgaccat 150106 ccgcagaaaa cttacctgtt ggcggctctt aggagagcgt ttgtcccgtt tgtatttcca 150166 ggagcggccg tggatcctac ggcggcgtct gcttctgggg accaccctgt actcgttggc 150226 gccaggcgaa tagcgcgtgc cacgagggag ctggaagccg cagcgaaaga gccacctgcg 150286 gcaaccccgg aaaaactttc ctcttcggcg ctattgcgcc atactagaac ttgcattgtt 150346 gctgccgcag atctcatggc ggcgcttact gctatagact gcccaccagt tacaataaac 150406 cccgccaagc gtcgcccgga tctgtatcaa gcactctcga gggcgcccaa aacagtcttt 150466 acgtgggagc gcgccatgcg ctactgcgcg gcttcgctat ggagtcgcct cgtcataggg 150526 tccctgccgc tgccatctac cgatgatgtc actcttcttg tagaagccat ggaagctgcc 150586 gtgggcgtgc ttggctgctc cgacgagggt gacgtgcggc cttcaattgc ctacgcggcc 150646 tctggttgga ggatgatcgc taccatgcta ggagatgatt ccgcgcccgg cagcgagcac 150706 gtcacggacg attgtcccgc agatgatgac acccaccacc atgacaggcg caaaagacga 150766 ggcggccggc ggagcacttg ccggtacgac ctacctcctg gcactcccga tcgcgcggcc 150826 cacctcctac tctacgggag tcctggcggc ggcaacggag cagagccttc ggctgcccaa 150886 gaatctcccg ccaacccctg gcccagagct ccaccgtgcg atgaacagga gccattatct 150946 gtgtcaccgt acgggcccga acccgaccgc cctccagatg atgactttga gacccgtaaa 151006 ggtctgaaaa gaaagtcatc tgaagatcac gcggacccga tcccagaagg caacgcgact 151066 aaaaaaacat gtggcctcca gggcttacca gattcattac ctccagcggt tccagaaaca 151126 gatagagaca acctcctcct ttcgccatgc cccataactc ccgagggtcc accatgtccg 151186 ccccgagaag aaccgcaaga accacaatcc ccgtcctttc acatttccga gataggtgag 151246 gccctgtttc attctacccc aatcagccct accatcctgt tcgcaccgga gggtttcatc 151306 ccgaaccaag gggatgttta ccgtccccat ggcaaaaact tgggcgttgc gctctccgca 151366 ggggagccat acgtgcccgc ccatcgcgcg cgtcctgctt tggagcggct cgcgaactat 151426 ttgcgcgggt tgagcagcgg tccggcggga agctattccg cgaccggacc cgcagattgg 151486 ctacctgctt ttgcgaggga cccagtagcc ctgggggagt tttgcaagag gatttcaccc 151546 gtgaacagag gggggcgctg cgtcttagag gagcggcttt cctggacgtt gcgacatccg 151606 gctgacaaaa tctatgacct catcattctt gtctcagaac gtgccccggg cgaattttta 151666 gaacgcgcgt acgcctacgc ggccctctgc ggacgtgcct gcatgccggg cggtgcctgt 151726 tggccagaat cgtggacacg cggggagtat ccctatatcc ctcccgacga gaaagacccg 151786 ggccgcagcg tggccattct ctgtacgaca gaccttggat acgccggggc ggtagaatgg 151846 atgcttgagc gcgctctcag tacatctggg cgcgcatgcg tggtcgtaga tgctagggat 151906 cccggggacg aggattcccg ctcgggtccg cgcataccta ccgggcggag gggtgttgtc 151966 tatgcccgga ctagcccccc gtcgcacgct taccttgtaa aactgtttac cggtccggcg 152026 gccgaacggt tggaacgggg tgggctcaat gtaggtcacg tagtaatgga caggcgactc 152086 actgtctcat ggcccctcag cgttgggaag accgacaacg acgatgaggg tacaggagac 152146 agagagaagt gtcctcccgt taagcacccc ttcctcactc acgtaactct caggagatta 152206 cgcgacgttg ctcgtgccat ggatgccggg tggagccact ccgaaatcgg aaaagcttca 152266 gccgtcttgt atccgtacat ggcccagccc catgtgggcg ccggggcgat gccgccatgt 152326 ccggaccttt ccgagtccag ttccactatg cattcttctt cgtcgtcttc ctcttcctca 152386 tgctcctcgt cgtcgtcttc ttcagactcc agctcatctg aagaagacgg ggacgagaag 152446 aacgagaaag aagatcgtga gcgcgccgga ggtggaaagc ggcgcggccg ccagcgcctc 152506 ccgattcggg accgcgtgta ccgtggccgg gactaccgcg tgcgcactcc ctgtgctgat 152566 attgtgggag gcacttctgc ttcctttagg gccgtcgctg cgtggggggg ggacacggta 152626 attcccgccc tccctctcgc agcgtcctgg tccgatccca gcaaaattcc acaggaagtt 152686 ctccgcatca tctccgatta ttacccggat gcgccagggg cgcgggcgcg tcggaacccg 152746 cttcacgtgg aaggactagc gcttatgcgt gccagaaatc ccgcccctct cgcattgcta 152806 cttggtgacg actattccca ttatcacact ccccgcaatc ggtccccaat gtgttgttgc 152866 tggagtgctc cgcggggagc aacgccagag tcacccagat gcaccggaca cacgcgcacc 152926 aggcgcccaa ggctaaccgt ctcctcaggg aggggggaaa ctagaaccct tgtacccaga 152986 ttagactctt cagaggatag gcggtcgcgt caacgccata gacgcatgac taactaccct 153046 ctaccgcatc ccactgtcac tgacccttgg tggtaaccgt ggtttccgtt attaacttaa 153106 cttattaaaa ctttttgtac aatttacttc tggttgcgtg ctcattcttt tgctagtcgg 153166 agcttgtcag ccaccgacga cttgtagaaa aagaccgggg aaatgatcta gccccgttga 153226 ctttagcttt gctgaacatt ctacccgtgc ggccatggag tgatattaga aaagggacga 153286 gaatgggggc aagtacgaat atcgggagga ggcggaacga ggtgttaagt aatgcacttg 153346 aatatttaat tcgttcattt tgatacataa gatccacgga agcttgtaat gctggccggc 153406 agcattatta ctattgcaag cgccgatata aaaatcgcaa tcaaaatact agattgtcta 153466 gtattgcctg ctctgacgga cgtaacgtct ctgtagggcg ttcgccaggt cattcaggaa 153526 cgacttcatc aaatacgaat cctccactgt tatagggcac ggtgcctgta ataaatagga 153586 gatacaatgt tagactcgtc atgcgaccaa taaggaccgc gtgagcacgg agacaaaaga 153646 tgtctcggag atactgtgct gcgcgttaga tagtctcttc aaagtacaga atgcgcgatg 153706 ctgccattat cttaccttgc gagagctacc gccattactg acagaattca gcacgtaata 153766 caaattgacg tagagcccct gcagtttttt gtgacactgt tggtgtttca aaatgaccga 153826 aagggattgg cacaactctt cagtttcgtt tagacgctgc aggaaaagca agattcgtta 153886 ataaaccgcc gagcagatta ttgggcaggg tagacctaag caaaaaatct acatttttaa 153946 tctggggcta gcgcgcggcc ggcctccagg gcattgccgt tgagaacatg tgacaaatga 154006 gctggctttt actcaccgtg tcggacgtaa agatgttcgg aactcttcta ccgtcgcagg 154066 aacccttata aggaaacgag agagcctaca gttatgaacg tgaacataga ctgaatagcc 154126 gtacatagtt acaaagaccg tgcaggcatg tcagcatggt ccaacgagaa tgagttgcct 154186 tacgtccttc tgcacacggt gcagtattct gatgctctcc gccagtaact tcaagggtgc 154246 cgcgcgtata ctgcgagcat ccccgactcc aagtagtaac accagtaacg ccagtgcggc 154306 ggacaatgga ggcttcgact tgccaggcat tcccaccgcg cgcaaccgcc gccgcgaaac 154366 ttctccccta gttctccctc tgggttcttc cctaaggata cactgccacc atttatatag 154426 ctttacgtgg cgggaaagcc ctagccccca ctatacccgg aagcggctac actagagccc 154486 ttgcaagagc agaaaatttt cccgccgtgg gttgcgcgtg aacaacttac cagaacgaga 154546 atttttgaaa cctctgagac tcacagcacg tgcttacgaa taccagaaac tgggagcacg 154606 gtgcttccgc gacactctac aaagataagt gcgggcacag ttaggtgcta ctgcgcttag 154666 ctaagtgctt aacctgagta gcacctaatt gcggccttag gccaaggtgt cgttacccgg 154726 atagctttca catcgatagc gcgtattacc ttcggcctga caactgtcag ctacccaacc 154786 gctctgtctt ctttcaatcc ttgactatta taactcgcgg taaccggccg tggccacata 154846 gtgaggcggt tatacggaat aagacccccc cccccggggc cttcacgttc gtgtccgttc 154906 ttgccagtga gcgttgctgg aaatcgaaaa ttccagtgtg gcgcttattg tgcaagaacc 154966 acgggcgtga cctgtatgac tactgagcaa agcacctcgc gctgccgcga aacttacaaa 155026 cggggcgaac tgcgtgacga ttctgtgatc gcgcttcgag gtccagtcgg agcccaccgg 155086 tgcccacctg aaccttttca gctccggaaa aggctaaact ggaaaatcta gtagaactag 155146 tttcacggct gcccgcgctg accgtttgct ctccacgggt ggaagaactt gggggggggg 155206 gttggcggac gctggcgttt gatgtcgggc cgtcaagtat ctcgcaaccc cagaacctgt 155266 ccagcatcta atagtagggg tcggacatga aaccacaagg gattggaagg gcggggagca 155326 ctcaggggac tgggagggga cgggtcggag aaaaccgcgg accaccagtg cacacaccct 155386 gcatccttgg ctgctcatat atagcccccc cccgcaaaac acccccccgg gtttattgcc 155446 gacgacgggg ctgtggagc                                              155465

Claims

1. A method of producing a live attenuated infectious laryngotracheitis virus (ILTV), the method comprising serially passaging the ILTV in culture for at least 15 serial passages at a temperature of at least 25° C. and no greater than 35° C.

2. The method of claim 1 wherein ILTV is passaged for at least 30 serial passages.

3. The method of claim 1 wherein the ILTV is passaged in culture at a temperature of from 28° C. to 31° C.

4. The method of claim 2 wherein the ILTV is passaged in culture at a temperature of from 28° C. to 31° C.

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Patent History
Patent number: 10201604
Type: Grant
Filed: Oct 29, 2014
Date of Patent: Feb 12, 2019
Patent Publication Number: 20160263210
Assignee: UNIVERSITY OF DELAWARE (Newark, DE)
Inventors: Jack Gelb, Jr. (Landenberg, PA), Brian S. Ladman (Landenberg, PA), Miguel Ruano (Newark, DE)
Primary Examiner: Stacy B Chen
Application Number: 15/032,338