Method of hormone treatment for patients with symptoms consistent with multiple sclerosis

Abstract

A method for patients having symptoms consistent with multiple sclerosis comprising administering a regimen of doses of human growth hormone of less than 0.5 mg/day. In one aspect, the method includes replenishing one or more of melatonin, thymus, thyroid, adrenal and sex hormones to predetermined levels in conjunction with the human growth hormone.

Description

BACKGROUND OF THE INVENTION

[0001] 1. Field of the Invention

[0002] The invention relates to hormone therapy as a treatment for patients with symptoms consistent with multiple sclerosis.

[0003] 2. Background

[0004] It is known that the levels of a variety of hormones drop substantially with age. These include human growth hormone, sex hormones, pineal, adrenal, thyroid, and thymus hormones. Hormonal replenishment methods employed as anti-aging treatments have been developed. An example of such method is described in U.S. Pat. No. 8,855,920. The utility of this method in connection with certain degenerative diseases, often associated with hormonal deficiencies due to aging, is also known in the art.

[0005] In contrast, Multiple Sclerosis (MS) is not a disease associated with degenerative conditions associated with old age. Rather, MS strikes mainly young adults and is the most common neurologic cause of disability in that age group. Overall, MS affects approximately 300,000 Americans.

[0006] MS is thought to be an autoimmune illness, wherein the immune system mistakenly recognizes normal brain and spinal tissue as “foreign” and attacks them, resulting in inflammation and damage. Myelin, the insulative material surrounding axons in nerve cells, are particularly affected by MS. In patients, the inflammation disrupts myelin, sometimes destroying the axons thus impeding the connections between nerve cells. The region of demyelination and inflammation within the brain or spinal cord is called a “plaque” and ranges anywhere from millimeters to more than a centimeter in diameter.

[0007] Actual MS symptoms appear to reflect the number and severity of plaques as well as their location at important sites within the nervous system. Mild sensory symptoms, e.g., tingling, burning, itching, may result. Typically, however, no diagnosis of MS is made until severe symptoms develop, such as weakness, paralysis or numbness of limbs, loss of vision, imbalance, chronic pain or chronic fatigue. The long term consequences of MS can be very debilitating. In some studies, 50% of patients were disabled within ten years and fewer than two thirds were able to walk after thirty years.

[0008] In common diagnosis, MS is found by excluding all other potential conditions from consideration. Formerly diagnostic chemical tests of cerebro-spinal fluid determined the presence of MS. This, however, has given way in recent years to magnetic resonance imaging (MRI) scans of the brain as the preferred diagnostic method. Brain MRI scans are abnormal in the vast majority of MS victims. Areas of abnormal brightness, suggesting increased water content, appear on “T2 weighted” scans; dark areas on “T1 weighted” scans suggest focal areas of tissue destruction; and abnormal brightness after a dye is injected for enhancement into the patient's bloodstream suggest that inflammation has damaged the blood-brain barrier. Again, as these symptoms are present also in other neurologic conditions, the diagnosis of MS is not made from these symptoms alone. This diagnosis results by testing done over time.

[0009] Various drugs, including natural immune substances such as beta-interferon, have proved effective in treating the physical symptoms of MS and reducing their frequency. Recently studies with animals employing induced experimental autoimmune encephalomyelitis (EAE), an animal model of MS, has shown that growth factor hormones, including Insulin-like Growth Factor hormones (IGF) may promote myelin regeneration, reducing and sometimes eliminating inflammatory lesions and reducing other clinical deficits in EAE subjects. In one study adult female mice having induced EAE were injected subcutaneously with 0.6 mg of IGF over a ten day period. Another group of EAE-induced mice were given placebos. Those treated with IGF-I exhibited reduced deficits and reduced numbers and sizes of inflammatory, demyelinating and demyelinated lesions.

SUMMARY

[0010] The invention relates to a hormone replenishment method employed as a treatment for subjects with symptoms consistent with Multiple Sclerosis (MS). The method includes, in one aspect, administering a regimen of human growth hormone (HGH) of less than 0.5 mg per day.

BRIEF DESCRIPTION OF THE DRAWINGS

[0011] FIG. 1 illustrates the growth hormone decline in advancing years.

[0012] FIG. 2 illustrates the free testosterone levels in males throughout life.

[0013] FIG. 3 illustrates the levels of estrogen and progesterone in females before and after menopause.

[0014] FIG. 4 illustrates the melatonin levels produced throughout life.

[0015] FIG. 5 illustrates the DHEA levels for males and females over a life span.

[0016] FIG. 6 illustrates the thyroid hormone levels versus age.

[0017] FIG. 7 illustrates the thymus hormone levels versus age.

[0018] FIG. 8 presents the peak IGF-I levels in males before and after administration of IGF-I.

[0019] FIG. 9 presents the peak IGF-I levels in females before and after administration of IGF-I.

DETAILED DESCRIPTION

[0020] The invention relates to a method useful in treating symptoms consistent with MS. In one embodiment, the method includes administering a regimen of doses of HGH of less than 0.5 mg per day. In another embodiment, the method includes determining that the level of human growth hormone and at least two supplemental hormones are below optimal or predetermined physiological levels for an adult human, and establishing a regimen for the replenishment of the level of the deficient hormones to optimal or predetermined physiological levels. The supplemental hormones include the sex hormones, namely testosterone, progesterone, and estrogen, the melatonin hormone, the adrenal hormones, namely DHEA and pregnenolone, the thyroid hormone, and the thymus hormone.

[0021] One of the hormones that declines sharply with age is human growth hormone (HGH, GH, or somatotropin). FIG. 1 illustrates the growth hormone decline in years for an ordinary human.

[0022] HGH is a protein hormone secreted by the somatotropic cells of the anterior lobe of the pituitary gland. HGH secretes in a pulsatile manner throughout a 24-hour period. The pulsatile diurnal output of growth hormone is modulated by a pair of inner synergistic hypothalamus hormones, the growth hormone releasing hormone (GHRH) and growth hormone inhibiting hormone (GHIH) or somatostatin. GHRH and GHIH are synthesized in the hypothalamus and transported along with other messenger hormones to the pituitary gland by means of a short specialized portal vein network. GHRH is essentially a series of short pulses, clocked at about once a minute uniformly throughout a 24-hour day. GHIH, on the other hand, is a “gatekeeper” that is normally abundant, but occasionally low, allowing pulses of growth hormone to be released from the pituitary gland into the bloodstream. The base line level of growth hormone, as far as serum concentration is concerned, is ordinarily at or below detectable limits from hour to hour.

[0023] The major secretion of HGH occurs at night, one to two hours after the onset of deep REM sleep. Peek secretion levels are between 10-50 mg/ml.

[0024] It is known that physiological roles are probably due both to direct actions of HGH and indirect actions mediated by the peptide hormones known as somatomedins. Somatomedins are stimulated predominantly by the action of HGH and include Insulin-like Growth Factor-I (also known as IGF-I and somatomedin-C) and IGF-II. The major site of somatomedin secretions is the liver, but there is also some production at peripheral sites.

[0025] Proper human growth from infancy is contingent upon adequate growth hormone secretion. Growth hormone appears to affect the growth of virtually every organ and tissue in the body. In normal development, HGH and the somatomedins are responsible for many manifestations of normal growth. Growth hormone deficiency during the childhood growing period is manifested by profound short stature. This deficiency has been treated by human growth hormone supplements for many years. However, the scarcity of the source material for natural HGH (i.e., pituitary glands of cadavers) has limited investigations into other possible applications for HGH. Recently, bioengineered HGH or recombinant HGH has been developed with identical characteristics as the natural HGH and removed the previous investigation limitations.

[0026] In 1990, a group of researchers published a report that showed that the declining activity of the IGF-I access with advancing age may contribute to the decrease in lean body mass and the increase of mass of adipose tissue that occur with aging. “Effects Of Human Growth Hormone In Men Over 60 Years Old”, Rudman, D., M.D., et al., The New England Journal of Medicine, Vol. 323, No. 1, Jul. 5, 1990. Subsequent studies have shown that growth hormone increases bone mass in osteoporosis, reverses declining cardiac function, reverses declining pulmonary function, reverses the decline in immune function associated with aging, increases lean muscle mass, decreases the percentage of body fat, and increase the capacity for exercise. See Powrie, J. et al., “Growth Hormone Replacement Therapy For Growth Hormone-Deficient Adults”, Drugs Vol. 49, No. 5, pages 656-63, 1995; Rosen, T., et al., “Consequences Of Growth Hormone Deficiency In Adults And The Benefits And Risks Of Recombinant Human Growth Hormone Treatment”, Horm. Rees., Vol. 43, pages 93-99, 1995; and Hoffman, A. R., “Growth Hormone Therapy In The Elderly: Implications For The Aging Brain”, Psychoneuro-endocrinology, Vol. 17, No. 4, pages 327-33, 1992 (concluding that it is possible that chronic physiological GH and/or IGH-I replacement therapy might reverse or prevent some of the inevitable sequelae of aging).

[0027] Optimal or predetermined physiological levels for the hormones included in replenishment as an embodiment of the method of the invention are set forth in Table I. These levels are set based upon the average peak levels for the hormones which are present in adult humans at natural ages of optimal health, vigor and potency- usually in the second and third decades of the average life span.

[0028] Table I shows that the HGH is measured by the level of IGF-I. Serum levels of HGH are difficult to measure. IGF-I is directly dependent upon the secretion of HGH by the pituitary gland, so therefore there is a direct linkage between increased secretion of HGH and increased production of IGF-I. Table II presents the effects of the low dose at high frequency human growth hormone administration (low dose-high frequency) contemplated by the invention on IGF-I (somatomedin C) blood levels. FIGS. 8 and 9 present the peak IGF-I levels in males and females, respectively, before and after administration of IGF-I. FIGS. 8 and 9 show mean, minimum and maximum, and 25, 50, and 75 percentile for 204 males and 91 females, respectively. Table III shows the data presented in FIG. 8. Table IV shows the data presented in FIG. 9. 1 TABLE I METHOD OF TARGET LEVEL HORMONE ADMINISTRATION (&mgr;g/ml blood) HGH injection IGF-I = 350 free testosterone gel female =  3 male =  40 progesterone capsule 10-25 estrogen capsule 100-200 melatonin capsule 18-69 (at 3:00 am) DHEA capsule female = 400 male = 600 thyroid tablet T3 = 150-180 pregnenolone capsule 100-200 thymus hormone Subligual powder N/A

[0029] 2 TABLE II Effects of Human Growth Hormone Administration (low dose-high frequency) on Somatomedin C Blood Levels Somatomedin C. Blood Levels (mg/ml) Before hGH After hGH Increase Mean 233.8 384.5 61.0% STDEV 62.3 50.8 SEM 9.1 7.4 No. Patients 47.0 47.0 MAX 366.0 574.0 MIN 132.0 301.0 P < 0001

[0030] 3 TABLE III Males IFG MALES MALES MALES MALES PEAK LEVELS BEFORE AFTER CHANGE DAYS SAMPLE SIZE BEFORE 204 MEAN MEAN MEAN MEAN AFTER 204 192.3 266.7  74.4 118.5 MEDIAN MEDIAN MEDIAN MEDIAN 183 272.5  75.5  82 STDEV STDEV STDEV STDEV  61.7  75.1  73.2 110.3 MIN MIN MIN MIN  21  63 135  6 25%ile 25%ile 25%ile 25%ile 147 217  24  40 50%ile 50%ile 50%ile 50%ile 183 273  76  82 75%ile 75%ile 75%ile 75%ile 231 325 125 146 MAX MAX MAX MAX 361 396 258 639

[0031] 4 TABLE IV Females FE- FE- FE- FE- IFG MALES MALES MALES MALES PEAK LEVELS BEFORE AFTER CHANGE DAYS SAMPLE SIZE BEFORE 91 MEAN MEAN MEAN MEAN AFTER 91 162.2 236.3    74.0 133.4 MEDIAN MEDIAN MEDIAN MEDIAN 154 240    72 101 STDEV STDEV STDEV STDEV  61.5  75.1    73.6 113.8 SEM SEM SEM SEM  6.4  5.9    4.8  13.2 MIN MIN MIN MIN  41  52  −93  26 25%ile 25%ile 25%ile 25%ile 111 177    26  49 50%ile 50%ile 50%ile 50%ile 164 240    72 101 75%ile 75%ile 75%ile 75%ile 205 294   128 173 MAX MAX MAX MAX 311 378   231 534

[0032] The method of determining the level of the select group of hormones may be accomplished by a simple blood test. A patients' blood is evaluated for the hormone levels of the select group of hormones and those levels are compared to the optimal or predetermined physiological levels set forth in Table I. Based on the comparison of a patient's hormone levels with the optimal or predetermined physiological levels, a regimen is established for the patient for the replenishment of the level of deficient hormones to optimal physiological levels. It is further contemplated, that after the initial evaluation and the establishment of the regimen, a patient is monitored every 30 days, by a similar blood test, until the patient attains the optimal or predetermined physiological level, and the dosages of hormone supplementation are adjusted accordingly. Once the target levels are established, the regimen directs that the patient continue to follow the established dosage of supplemental hormones indefinitely to maintain the optimal or predetermined physiological levels. Periodic blood tests are subsequently administered to assure that the optimal or predetermined physiological levels are maintained.

[0033] The optimal or predetermined physiological level of HGH and the supplemental hormones are set forth in Table I. These levels recognize the importance of each hormone to the body and are based on studies that indicate the period of life when the individual hormones are at their peak in the human body—generally in the second and third decade of an average human life span.

[0034] The method of the invention also contemplates that the HGH is administered in keeping with the way the pituitary secretes HGH naturally. The low dose-high frequency method mimics the pituitary gland. The pituitary secretes HGH 24 hours a day, with the peaks during the first two hours of sleep. The method contemplates that the patients take lower doses more frequently to try to mimic the natural rhythm of the body as much as possible. The invention contemplates that the patients take HGH injections at least twice daily. A preferred regimen calls for subcutaneous injections of doses less than 0.5 mg per day (approximately 1-3 I.U.), administered twice daily. Typical doses for this regimen range from 4 units per week to 8 units per week.

[0035] Because the invention contemplates that human growth hormone is administered in lower doses that mimic the natural rhythm of the body, the invention contemplates that the patient experiences none of the adverse side effects reported in earlier studies when higher pharmacological doses were given to patients more intermittently, e.g., 3 days per week.

[0036] Aside from the administration of HGH the invention contemplates therapeutic application of supplemental hormones. The supplemental hormones include the sex hormones, namely testosterone, progesterone, and estrogen, the melatonin hormone, the adrenal hormones, namely DHEA and pregnenolone, the thyroid hormone, and the thymus hormone. Dosage for administration of the supplemental hormones varies with the particular hormone.

[0037] The optimal or predetermined physiological level goal in testosterone replacement therapy in men is to maintain a total testosterone level at about 900-1200 &mgr;g/ml and a free testosterone level of about 30-40 &mgr;g/ml throughout one's lifetime. FIG. 2 illustrates the free testosterone level in males throughout life and shows the level decreasing with advancing age. In women, the levels of testosterone to maintain are much less, specifically women should maintain a free testosterone level of about 3 &mgr;g/ml.

[0038] Possible side effects which can occur during testosterone replacement include some weight gain due to accumulation of lean body mass, fluid retention, and among patients taking supraphysiologic doses of androgens: decreased testicular size, azoospermia, and acne.

[0039] FIG. 3 illustrates the levels of estrogen and progesterone in females before and after menopause. As noted above, candidates for supplemental estrogen or progesterone should replenish these hormones concurrently to avoid the risk of side effects. These hormones may be taken in capsule form. The optimal or predetermined target physiological level of progesterone is 10-25 &mgr;g/ml blood, and of estrogen is 100-200 &mgr;g/ml blood.

[0040] FIG. 4 illustrates the melatonin levels produced throughout life. The optimal or predetermined target physiological level of melatonin is 18-69 &mgr;g/ml determined by the average level of melatonin produced by a healthy young adult (i.e., 20-25 years). Further, melatonin supplementation has been shown to be completely harmless to the body and cause no side effects. As noted above, the amount of the adrenal hormone DHEA peaks in the bloodstream of an individual at about 25 years of age. In one embodiment, FIG. 5 illustrates the DHEA levels for males and females over a typical life span. It is the peak physiological level that, in one embodiment, is the goal of the DHEA replenishment therapy. The optimal or predetermined physiological level of DHEA in a female is 400 &mgr;g/ml blood and in a male is 600 &mgr;g/ml blood.

[0041] The serum level of pregnenolone for normal adults ranges between 50 to 350 &mgr;g/ml of blood. If a person's level is low and falls outside of this range, replacement is recommended. Depending on the degree to which a patient may be deficient, the dose is approximately 100-200 milligrams (“mg”) each day. The older or less healthy a person is, the more likely he or she will feel a dramatic effect from pregnenolone. A blood test is required to determine each individual's need for pregnenolone replacement, and to assess the proper dosage for therapy.

[0042] Maintaining sufficient thyroid hormone levels is also beneficial. FIG. 6 illustrates thyroid hormone levels versus age. Supplementation with thyroid hormone, particularly T-3 to 150-180 &mgr;g/ml of blood is contemplated by the invention.

[0043] FIG. 7 illustrates thymus hormone level versus age. Laboratory results indicate that the presence of thymus hormone in circulation can have an effect on a variety of other organ systems. All four thymic preparations (i.e., thymosin, thymulin, THF, and thymopoietin) are commercially available. Such a replacement therapy is totally harmless. The Food and Drug Administration has not regulated thymic hormones because they are not yet considered drugs or pharmaceuticals. Thymus hormone is in the same status and classification as DHEA and melatonin hormones which are also available over the counter.

[0044] By maintaining the optimal or predetermined physiological levels of human growth hormone and the supplemental hormones described above, the treatment is effective for reducing symptoms commonly associated with MS. As noted in the Background of the Invention section, diagnosis of MS is by exclusion, i.e., all other alternative diagnoses of a demyelinating condition must be ruled out before a diagnosis of MS may be established. The following case studies of patients with demyelinating conditions demonstrated from testing are illustrative of the effectiveness of the invention in patients with symptoms consistent with MS:

[0045] Case 1: (43 year old male).

[0046] An MRI of the brain during the first exam in 1995 revealed multiple white matter signal abnormalities as well as subtle diffuse signal abnormalities consistent with MS. Soon after this test, the patient was placed on a hormone replenishment regimen, featuring twice daily subcutaneous doses of human growth hormone in the amount of 0.5 mg. per dose. Testosterone, melatonin, DHEA, thyroid, pregnenolone, and thymus hormone were also given in the manner previously described to optimal levels.

[0047] A later exam in 1998 noted significant diminishment of lesions, including the actual disappearance from the MRI scan of lesions. The examining physician noted that the previously noted large left middle cerebellar peduncle lesion was very subtle on the current film and was initially interpreted as normal. There was also a small lesion in the anterior limb of the internal capsule seen on the 1995 study which is not currently visualized. Finally, the examining physician noted that there was a right posterior frontal deep white matter lesion that was slightly smaller than previously noted in 1995 with the remaining lesions noted from the 1995 examination remaining essentially unchanged. Brain evoked response studies also indicate improvement in speed of neurotransmission after this treatment. For example, visual evoked responses may reveal optic nerve involvement by MS. Studies of the patient's visual evoked responses before and after hormone replenishment therapy revealed faster conduction speed after the therapy, indicating improvement in the optic nerve. The patient also regained complete motor strength and sensory disturbances disappeared.

[0048] Case 2: (Female 60 years of age):

[0049] Brain MRI scan taken in 1993 revealed multiple punctate foci of increased signal intensity on T2 weighted images in the corona radiata and basal ganglia and thalami, compatible with the presence of multiple lacunar infarcts (areas of necrotic tissue). The test also revealed lesions in the deep cerebral structures involving white matter and a lesion in the right side pons was observed to be approximately 1.0 cm in size. The conclusion by the examining physician was “thought extensive widespread infarcts could account for all the above described findings, the patient's age and sex makes this somewhat less likely and a demyeliting process, such as multiple sclerosis must be considered. Certainly this diagnosis could account for all the abnormal findings seen.”

[0050] In January of 1995, with no observed changes in her condition, the patient initiated hormone replenishment treatment including subcutaneous injection twice daily of 0.5 mg Human Growth Hormone, as well as supplemental hormones such as progesterone, estrogen, DHEA, melatonin and pregnenolone in doses previously described.

[0051] In October 1995 a further brain MRI showed the pontine lesion to decrease in size to approximately 0.4 cm (surrounded by a crescentric margin of subtle enhancement). With respect to other white matter foci, none showed abnormal enhancement. The examining physician concluded that the degree of enhancement had subsided considerably, as had the size of the individual pontine lesion. She also fully regained her motor strength and recovered from sensory disturbances.

[0052] The above case histories indicate that hormone replenishment and supplementation is effective at reducing the symptoms associated with MS. HGH administration, given in a low dose, high frequency regimen is believed particularly to be effective at reducing the symptoms of MS.

[0053] In the foregoing specification, the invention has been described with reference to specific exemplary embodiments thereof. It will, however, be evident that various modifications and changes may be made thereto without departing from the broader spirit and scope of the invention as set forth in the appended claims. The specification and drawings are, accordingly, to be regarded in an illustrative rather than a restrictive sense.

Claims

1. A method comprising:

in a human subject having symptoms consistent with multiple sclerosis, administering a regimen of doses of human growth hormone of less than 0.5 mg. per day.

2. The method of

claim 1, wherein administering the regimen of human growth hormone comprises subcutaneous injections of doses of human growth hormone.

3. The method of

claim 2, wherein the regimen for injections comprises twice daily.

4. The method of

claim 1, further comprising prior to administering a regimen of human growth hormone measuring the level of human growth hormone in a human subject's blood to determine that the level of human growth hormone is below a predetermined physiological level for an adult human.

5. The method of

claim 4, further comprising measuring a level of supplemental hormones selected from the group consisting of sex hormone, melatonin hormone, adrenal hormone, thyroid hormone and thymus hormone to determine that at least one of the hormones is below a predetermined level for an adult human; and replenishing the level of the at least one supplemental hormone to a predetermined physiological level.

6. A method comprising:

measuring a sample of a human subject's blood, the human subject having symptoms consistent with multiple sclerosis, to determine that the level of human growth hormone is below a predetermined physiological level for an adult human; and

replenishing the level of human growth hormone to the predetermined physiological level.

7. The method of

claim 6, wherein replenishing the level of human growth hormone comprises subcutaneous injections of doses of human growth hormone.

8. The method of

claim 7, wherein a replenishment regimen comprises injections twice daily.

9. The method of

claim 6, further comprising measuring a level of supplemental hormones in the human subject's blood, the supplemental hormones selected from the group consisting of sex hormone, melatonin hormone, adrenal hormone, thyroid hormone and thymus hormone to determine that at least one of the hormones is below a predetermined level for an adult human; and

replenishing the level of the at least one supplemental hormone to a predetermined physiological level.

10. A kit for treating symptoms associated with multiple sclerosis comprising human growth hormone and at least one of the supplemental hormones selected from the group consisting of sex hormone, melatonin hormone, adrenal hormone, thyroid hormone, and thymus hormone, the kit for establishing a regimen for the replenishment of the human growth hormone and the at least one of the supplemental hormones to predetermined physiological levels.

11. The kit of

claim 10, wherein the amount of human growth hormone is provided in intravenous unit form in doses of less than 0.5 mg per day.

12. The kit of

claim 11, wherein the amount of human growth hormone is administered twice daily.

13. The kit of

claim 10, wherein the sex hormone comprises at least one of testosterone, progesterone, and estrogen.