Anti-inflammatory indole derivatives

Abstract

Therapeutic compounds of formula (I) wherein X is CH2 or SO2; R1 is an optionally substituted aryl or heteroaryl ring; R2 and R3 are various specified groups, R4 is a group NHCOR15, NHSO2R15 or OCONR16R17 where R15 is optionally substituted alkyl, optionally substituted aryl or optionally substituted heteroaryl and R16 and R17 are independently selected from hydrogen, optionally substituted alkyl, optionally substituted aryl and optionally substituted heteroaryl, with the proviso that at least one of R16 or R17 is other than hydrogen, or R16 and R17 together with the nitrogen atom to which they are attached from an optionally substituted heterocyclic ring which optionally contains further heteroatoms; and R5, R6 and R7 are independently selected from hydrogen, a functional group or an optionally substituted hydrocarbyl groups or optionally substituted heterocyclic groups; and further provided that when R4 is a group NHCOR15, R15 is substituted alkyl, optionally substituted aryl or optionally substituted heteroaryl; as well as pharmaceutical compositions containing them are described and claimed. These compounds and compositions are useful in the treatment of disease mediated by monocyte chemoattractant protein-1 or RANTES (Regulated Upon Activation, Normal T-cell Expressed and Secreted), such as inflammatory disease.

Description

The present invention relates to chemical compounds, to their production as well as to pharmaceutical compositions containing them as well as to their use in therapy, in particular of inflammatory disease.

MCP-1 is a member of the chemokine family of pro-inflammatory cytokines which mediate leukocyte chemotaxis and activation. MCP-1 is a C—C chemokine which is one of the most potent and selective T-cell and monocyte chemoattractant and activating agents known. MCP-1 has been implicated in the pathophysiology of a large number of inflammatory diseases including rheumatoid arthritis, glomerular nephritides, lung fibrosis, restenosis (International Patent Application WO 94/09128), alveolitis (Jones et al., 1992, J. Immunol., 149, 2147) and asthma. Other disease areas where MCP-1 is thought to play a part in their pathology are atherosclerosis (e.g. Koch et al., 1992, J. Clin. Invest., 90, 772-779), psoriasis (Deleuran et al., 1996, J. Dermatological Science, 13, 228-236), delayed-type hypersensitivity reactions of the skin, inflammatory bowel disease (Grimm et al., 1996, J. Leukocyle Biol., 59, 804-812), multiple sclerosis and brain trauma (Berman et al, 1996, J. Immunol., 156, 3017-3023). An MCP-1 inhibitor may also be useful to treat stroke, reperfusion injury, ischemia, myocardial infarction and transplant rejection.

MCP-1 acts through the MCP-1 receptor (also known as the CCR2 receptor). MCP-2 and MCP-3 may also act, at least in part, through the MCP-1 receptor. Therefore in this specification, when reference is made to “inhibition or antagonism of MCP-1” or “MCP-1 mediated effects” this includes inhibition or antagonism of MCP-2 and/or MCP-3 mediated effects when MCP-2 and/or MCP-3 are acting through the MCP-1 receptor.

Copending International Patent Application Nos. PCT/GB98/02340 and PCT/GB98/02341 describe and claim groups of compounds based upon the indole ring structure which arc inhibitors of MCP-1 and therefore have applications in therapy.

The use of certain indole derivatives as NMDA antagonists is described is U.S. Pat. No. 5,051,442, WO93 12780, EP-483881. Other indoles and their use as inhibitors of leukotriene biosynthesis is described in for example, EP-A-275-667.

The applicants have found a particular substitution on the indole ring produces advantageous results when used therapeutically as inhibitors of MCP-1.

According to the present invention there is provided a compound of formula (I)
X is CH₂ or SO₂

• R¹ is an optionally substituted aryl or heteroaryl ring;
• R² is carboxy, cyano, —C(O)CH₂OH, —CONHR⁸, —SO₂NHR⁹, tetrazol-5-yl, SO₃H, or a group of formula (VI)
  where R⁸ is selected from hydrogen, alkyl, aryl, cyano, hydroxy, —SO₂R¹² where R¹² is alkyl, aryl, heteroaryl, or haloalkyl, or R⁸is a group —(CHR¹³)_r—COOH where r is an integer of 1-3 and each R¹³ group is independently selected from hydrogen or alkyl; R⁹ is hydrogen, alkyl, optionally substituted aryl such as optionally substituted phenyl or optionally subtituted heteroaryl such as 5 or 6 membered heteroaryl groups, or a group COR¹⁴ where R¹³ is alkyl, aryl, heteroaryl or haloalkyl; R¹⁰ and R¹¹ are independently selected from hydrogen or alkyl, particularly C_1-4 alkyl;
• R³ is hydrogen, a functional group, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted alkoxy, optionally substituted aralkyl, optionally substituted aralkyloxy, optionally substituted cycloalkyl;

R⁴ is a group NHCOR¹⁵, NHSO₂R¹⁵ or OCONR¹⁶R¹⁷ where R¹⁵ is optionally substituted alkyl, optionally substituted aryl or optionally substituted heteroaryl and R¹⁶ and R¹⁷ are independently selected from hydrogen, optionally substituted alkyl, optionally substituted aryl and optionally substituted heteroaryl, with the proviso that at least one of R¹⁶ or R¹⁷ is other than hydrogen, or R¹⁶ and R¹⁷ together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring which optionally contains further heteroatoms; and

R⁵, R⁶ and R⁷ are independently selected from hydrogen, a functional group or an optionally substituted hydrocarbyl groups or optionally substituted heterocyclic groups.

Suitably, where R⁴ is a group NHCOR¹⁵, R¹⁵ is substituted alkyl, optionally substituted aryl or optionally substituted heteroaryl.

Compounds of formula (I) are inhibitors of monocyte chemoattractant protein-1. In addition, they appear to inhibit RANTES (Regulated upon Activation, Normal Tcell Expressed and Secreted), induced chemotaxis. RANTES is another chemokine from the same family as MCP-1, with a similar biological profile, but acting though the CCR1 receptor. As a result, these compounds can be used to treat disease mediated by these agents, in particular inflammatory disease.

In this specification the term ‘alkyl’ when used either alone or as a suffix includes straight chained, branched structures. These groups may contain up to 10, preferably up to 6 and more preferably up to 4 carbon atoms. Similarly the terms “alkenyl” and “alkynyl” refer to unsaturated straight or branched structures containing for example from 2 to 10, preferably from 2 to 6 carbon atoms. Cyclic moieties such as cycloalkyl, cycloalkenyl and cycloalkynyl are similar in nature but have at least 3 carbon atoms. Terms such as “alkoxy” comprise alkyl groups as is understood in the art.

The term “halo” includes fluoro, chloro, bromo and iodo. References to aryl groups include aromatic carbocylic groups such as phenyl and naphthyl. The term “heterocyclyl” includes aromatic or non-aromatic rings, for example containing from 4 to 20, suitably from 5 to 8 ring atoms, at least one of which is a heteroatom such as oxygen, sulphur or nitrogen. Examples of such groups include furyl, thienyl, pyrrolyl, pyrrolidinyl, imidazolyl, triazolyl, thiazolyl, tetrazolyl, oxazolyl, isoxazolyl, pyrazolyl, pyridyl, pyrimidinyl, pyrazinyl, pyridazinyl, triazinyl, quinolinyl, isoquinolinyl, quinoxalinyl, benzothiazolyl, benzoxazolyl, benzothienyl or benzofuryl.

“Heteroaryl” refers to those groups described above which have an aromatic character. The term “aralkyl” refers to aryl substituted alkyl groups such as benzyl.

Other expressions used in the specification include “hydrocarbyl” which refers to any structure comprising carbon and hydrogen atoms. For example, these may be alkyl, alkenyl, alkynyl, aryl, heterocyclyl, alkoxy, aralkyl, cycloalkyl, cycloalkenyl or cycloalkynyl.

The term “functional group” refers to reactive substituents. They may comprise electron-donating or electron-withdrawing. Examples of such groups include halo, cyano, nitro, C(O)_nR¹⁸, OR¹⁸, S(O)_nR¹⁸, NR¹⁹R²⁰, C(O)NR¹⁹R²⁰, OC(O)NR¹⁹R²⁰, —NR¹⁹C(O)_nR¹⁸, —NR¹⁸CONR¹⁹R²⁰, —N═CR¹⁹R²⁰, S(O)_mNR¹⁹R²⁰ or —NR¹⁹S(O)_mR¹⁸ where R¹⁸, R¹⁹ and R²⁰ are independently selected from hydrogen or optionally substituted hydrocarbyl, or R¹⁹ and R²⁰ together form an optionally substituted ring which optionally contains further heteroatoms such as S(O)_m, oxygen and nitrogen, n is an integer of 1 or 2, m is 1 or 2.

Suitable optional substituents for hydrocarbyl groups R¹⁸, R¹⁹ and R²⁰ include halo, perhaloalkyl such as trifluoromethyl, mercapto, hydroxy, carboxy, alkoxy, heteroaryl, heteroaryloxy, alkenyloxy, alkynyloxy, alkoxyalkoxy, aryloxy (where the aryl group may be substituted by halo, nitro, or hydroxy), cyano, nitro, amino, mono- or di-alkyl amino, oximino or S(O)_nR^x where n is as defined above and R^x is alkyl such as C_1-4 alkyl.

Suitable substituents for these hydrocarbyl or heterocylic groups include those listed above for R¹⁸, R¹⁹ and R²⁰.

Suitably R¹ is an optionally substituted phenyl, pyridyl, naphthyl, furyl or thienyl ring, and in particular is a substituted phenyl or pyridyl ring.

Suitable optional substitutents for R¹ in formula (I) include alkyl, alkenyl, alkynyl, halo, haloalkyl including perhaloalkyl such as trifluoromethyl, mercapto, alkoxy, haloalkoxy, alkenyloxy, alkynyloxy, hydroxyalkoxy, alkoxyalkoxy, alkanoyl, alkanoyloxy, cyano, nitro, amino, mono- or di-alkyl amino, oximino, sulphonamido, carbamoyl, mono or dialkylcarbamoyl or S(O)_mR²¹ where m is as defined above and R²¹ is hydrocarbyl.

Particular examples of substituents R⁵, R⁶ and R⁷, and where appropriate also R⁴ include hydrogen, hydroxy, halo, optionally substituted alkyl such as aralkyl, carboxyalkyl or the amide derivative thereof; alkoxy; aryloxy; aralkyloxy; or an amino group which is optionally substituted with alkyl, aryl or aralkyl. A specific functional group which is suitable for R⁴, R⁵, R⁶ and/or R⁷ is a group of sub-formula (IV).

Particular examples of groups R⁵, R⁶ and R⁷ are hydrogen, hydroxy, halo or alkoxy. In particular R⁶ and R⁷ are hydrogen. R⁵ may be hydrogen but in addition is suitably a small subsitutent such as hydroxy, halo or methoxy.

Particular substituents for R¹ include trifluoromethyl, C_1-4alkyl, halo, trifluoromethoxy, C_1-4alkoxy, C_1-4alkanoyl, C_1-4alkanoyloxy, nitro, carbamoyl, C_1-4alkoxycarbonyl, C_1-4alkylsulphanyl, C_1-4alkylsulphinyl, C_1-4alkylsulphonyl, sulphonamido, carbamoylC_1-4alkyl, N—(C_1-4alkyl)carbamoylC_1-4alkyl, N—(C_1-4alkyl)₂carbamoyl-C_1-4alkyl, hydroxyC_1-4alkyl or C_1-4alkoxyC_1-4alkyl.

Additionally or alternatively, two such substituents together may form a divalent radical of the formula —O(CH₂)_1-4O— attached to adjacent carbon atoms on the R¹ ring.

Preferred substitutents for R¹ are one or more non-polar substituents such as halo.

In particular, R¹ is substituted by one or more halo groups, in particular chlorine. A particular example of an R¹ group is 3,4-dichlorophenyl, 3-fluoro-4-chlorophenyl, 3-chloro-4-fluorophenyl or 2,3-dichloropyrid-5-yl.

Examples of groups R² include carboxy; cyano; tetrazol-5-yl; SO₃H; —CONHR⁸ where R⁸ is selected from cyano, hydroxy, —SO₂R¹² where R¹² is alkyl such as C_1-4alkyl, aryl such as phenyl, heteroaryl or trifluoromethyl, or R⁸ is a group —(CHR¹⁰)_r—COOH where r is an integer of 1-3 and each R¹⁰ group is independently selected from hydrogen or alkyl such as C_1-4 alkyl; or R² is a group —SO₂NHR⁹ where R⁹ is an optionally substituted phenyl or an optionally substituted 5 or 6 membered heteroaryl group, or a group COR¹⁴ where R¹⁴ is alkyl such as C_1-4alkyl, aryl such as phenyl, heteroaryl or trifluoromethyl, or R² is a group of formula (VI)
where R¹⁰ and R¹¹ are independently selected from hydrogen or alkyl, particularly C_1-4 alkyl.

Preferably R² is carboxy or a pharmaceutically acceptable salt or ester thereof.

Suitable groups R³ include hydrogen, fluoro, chloro, bromo, iodo, methyl, cyano, trifluoromethyl, hydroxymethyl, alkoxyalkyl such as C_1-4alkoxymethyl, methoxy, benzyloxy, carboxyalkoxy such as carboxymethoxy, methylsulphanyl, methylsulphinyl, methylsulphonyl or carboxyC_3-6cycloalkyl, —(CHR²²)_r—NR²³R²⁴ (where r is 0-2, each R²² is independently hydrogen or alkyl, in particular C_1-4alkyl, R²³ and R²⁴ are independently selected from H and C_1-4alkyl or R²³ and R²⁴ together with the nitrogen to which they are attached form a 5 or 6 membered ring optionally containing one further heteroatom selected from O, N, S, S(O) or SO₂. Suitably R²³ and R²⁴ together form a heterocylic ring such as morpholino or piperazinyl.

Other such groups R³ include optionally substituted aryl groups, such as optionally substituted phenyl or naphthyl group. Suitable substituents for phenyl groups R³ include one or more groups selected from chlorine, fluorine, methyl, trifluoromethyl, trifluoromethoxy, amino, formyl, phenyl, methoxy, phenoxy or phenyl.

R³ may comprise a range of substituents as listed above, in particular, hydrogen or a small substituent group such as C_1-4alkyl in particular methyl, or trifluoromethyl, and is preferably hydrogen.

Suitable optional substitutents for the group R¹⁵, R¹⁶ and R¹⁷ as they appear in the definition of R⁴, include functional groups as hereinbefore defined, as well as aryl or heterocyclyl groups, either of which may themselves be substituted by one or more functional groups or further aryl or heterocyclyl groups.

Particular examples of substituents for groups R¹⁵, R¹⁶ and R¹⁷ include one or more groups selected from halo such as chloro; hydroxy, cyano; amino, mono- or di-alkylamino; C_1-4 alkoxy; carboxy; sulphonamido; CONH₂; alkylamido where the alkyl moiety is optionally substituted for example with a functional groups such as carboxy; morpholino; pyridyl; pyrimidinyl; phenyl optionally substituted by halo such as chloro, hydroxy, alkoxy such as methoxy, carbamoyl, acyl such as acetyl, or hydroxyalkyl where the alkyl group suitably includes at least two carbon atoms, such as hydroxyethyl. Other examples of subsitutents for phenyl groups R¹⁵ is alkanoylamino group such as methoylamino.

Where R¹⁵, R¹⁶ and/or R¹⁷ is a heterocyclyl group, or where R¹⁶ and R¹⁷ together form an optionally substituted heterocyclic ring, these may be substituted by functional groups such as halo or hydroxy, or by alkyl groups such as methyl or ethyl, or alkenyl or alkynyl groups any of which may be subsituted, for example with hydroxy, as well as with further heteroaryl groups such as pyridyl. Particular examples of heterocyclic groups R¹⁵, R¹⁶ and/or R¹⁷ are optionally substituted thiophenyl, optionally substituted imidazolyl, optionally subtituted pyridyl.

Thus thiophenyl groups R¹⁵, R¹⁶ and/or R¹⁷ may comprise pyridyl-thiophenyl, whilst an example of a substituted imidazolyl group for R¹⁵, R¹⁶ and/or R¹⁷ is methylimidazolyl and halopyridyl in particular chloropyridyl is an example of a substituted pyridyl moiety for these groups.

Particular examples of R¹⁵ include alkyl in particular methyl optionally substituted by a functional groups or, in particular, a heterocyclyl group where the heterocyclyl group may be optionally substituted by a functional group such as halo or hydroxy or by an alkyl group such as methyl. Preferably, R¹⁵ is a substituted alkyl group. Where the substitutent is a functional group, it is preferably a group of formula NR¹⁹R²⁰ where R¹⁹ and R²⁰ are as defined above. Thus examples of substituted alkyl groups R¹⁵ include morpholinomethyl or alkyl such as methyl substituted with a substituted alkyl amino group wherein the substitutents include carboxy, alkanoyl, phenyl or alkyl sulphonyl.

Other examples of R¹⁵ are heterocylcyl groups which are optionally subsitituted for example by alkyl such as methyl, functional groups such as chloro or heterocycyl groups such as pyridyl.

Particular examples of R¹⁶ and R¹⁷ are alkyl such as methyl.

X is CH₂ or SO₂ and is preferably CH₂.

Suitable pharmaceutically acceptable salts of compounds of formula (I) include acid addition salts such as methanesulfonate, fumarate, hydrochloride, hydrobromide, citrate, maleate and salts formed with phosphoric and sulphuric acid. In another aspect suitable salts are base salts such as an alkali metal salt for example sodium, an alkaline earth metal salt for example calcium or magnesium, an organic amine salt for example triethylamine, morpholine, N-methylpiperidine, N-ethylpiperidine, procaine, dibenzylamine, N,N-dibenzylethylamine or amino acids for example lysine. There may be more than one cation or anion depending on the number of charged functions and the valency of the cations or anions. A preferred pharmaceutically acceptable salt is a sodium salt.

An in vivo hydrolysable ester of a compound of the formula (I) containing carboxy or hydroxy group is, for example, a pharmaceutically acceptable ester which is hydrolysed in the human or animal body to produce the parent acid or alcohol.

Suitable pharmaceutically acceptable esters for carboxy include alkyl esters, such as C_1-6 alkyl esters for example, ethyl esters, C_1-6alkoxymethyl esters for example methoxymethyl, C_1-6alkanoyloxymethyl esters for example pivaloyloxymethyl, phthalidyl esters, C_3-8cycloalkoxy-carbonyloxyC_1-6alkyl esters for example 1-cyclohexylcarbonyloxyethyl; 1,3-dioxolen-2-onylmethyl esters for example 5-methyl-1,3-dioxolen-2-onylmethyl; and C_1-6alkoxycarbonyloxyethyl esters for example 1-methoxycarbonyloxyethyl and may be formed at any carboxy group in the compounds of this invention.

Suitable pharmaceutically acceptable esters of compounds of formula (I) are in vivo hydrolysable ester of a compound of the formula (I) containing a hydroxy group includes inorganic esters such as phosphate esters and α-acyloxyalkyl ethers and related compounds which as a result of the in vivo hydrolysis of the ester breakdown to give the parent hydroxy group. Examples of α-acyloxyalkyl ethers include acetoxymethoxy and 2,2-dimethylpropionyloxymethoxy. A selection of in vivo hydrolysable ester forming groups for hydroxy include alkanoyl, benzoyl, phenylacetyl and substituted benzoyl and phenylacetyl, alkoxycarbonyl (to give alkyl carbonate esters), dialkylcarbamoyl and N-(dialkylaminoethyl)-N-alkylcarbamoyl (to give carbamates), dialkylaminoacetyl and carboxyacetyl.

Esters which are not in vivo hydrolysable are useful as intermediates in the production of the compounds of formula (I) and therefore these form a further aspect of the invention.

Thus examples of compounds of formula (I) include the following:

TABLE 1
Compd
No.	R3	R4	R5	R6	Ra	Rb
1	H		H	H	H	H
2	H		H	H	Cl	Cl
3	H		H	H	Cl	Cl
4	H		H	H	Cl	Cl
5	H		H	H	Cl	Cl
6	H		H	H	Cl	Cl
7	H		H	H	Cl	Cl
8	H	NHC(O)CH2NHCH2COOH	H	H	Cl	Cl
9	H		H	H	Cl	Cl
10	H	OC(O)N(CH3)2	H	H	Cl	Cl
11	H		H	H	Cl	Cl
12	H		H	H	Cl	Cl
13	H		H	H	Cl	Cl
14	H	NHC(O)CH2N(CH3)CH2COOH	H	H	Cl	Cl
15	H		H	H	Cl	Cl
where * indicates the point of attachment of the group to the indole ring.

Compounds of formula (I) are suitably prepared by methods such as those described in International Patent Application Nos. PCT/GB98/02340 and PCT/GB98/02341.

In particular compounds of formula (I) where R⁴ is NHCOR¹⁵ or NHSO₂R¹⁵ can be prepared by reacting a compound of formula (VII)
where X, R¹, R³, R⁵, R⁶ and R⁷ are as defined in relation to formula (I). R² is a group R² as defined in relation to formula (I) or a protected form thereof, with a compound of formula (VIII)
Z-R²²  (VIII)
where Z is a leaving group and R²² is a group COR^15′ or SO₂R^15′ where R^15′ is group R¹⁵ as defined in relation to formula (I) or a precursor thereof;
and thereafter if desired or necessary:

• (i) converting a precursor group R^15′ to a group R¹⁵ and/or converting a group R¹⁵ to a different such group;
• (ii) deprotecting a group R^2′ to a group R².
  • Suitable leaving groups Z include halo such as chloro.
    The reaction is suitably effected in an organic solvent such as dichloromethane or tetrahydrofuran in the presence of a base such as triethylamine or pyridine. Moderate temperatures, for example from 0° to 50° C. and conveniently ambient temperature, are employed in the reaction.

Compounds of formula (I) where R⁴ is a group OCONR¹⁶R¹⁷ may be prepared by a broadly similar method by reacting a compound of formula (VIIA)
where X, R^2′, R1, R³, R⁵, R⁶ and R⁷ are as defined in relation to formula (I), R² is a group R² as defined in relation to formula (I) or a protected form thereof, with a compound of formula (VIIIA)
Z-CONR¹⁶R¹⁷  (VIIIA)
where Z, R¹⁶ and R¹⁷ are as defined above.

Compounds of formula (VIIA) can be prepared by reacting a compound of formula (IX)
where R³, R⁵, R⁶ and R⁷ are as defined in relation to formula (I) and R^2′ is as defined in relation to formula (VII) and R⁴⁰ is a protecting group; with compound of formula (X)
R¹—X-Z¹  (X)
where R¹ and X are as defined in relation to formula (I) and Z¹ is a leaving group, and thereafter, removing the protecting group R⁴⁰.

Suitable leaving groups for Z include halide such as chloride, bromide or iodide, as well as mesylate or tosylate. The reaction is suitably effected in an organic solvent such as dimethylformamide (DMF) tetrahydrofuran (THF) or DCM in the presence of a base such as sodium hydride, sodium hydroxide, potassium carbonate. Optionally the reaction is effected in the presence of a suitable phase transfer catalyst. The choice of base and solvent is interdependent to a certain extent in that certain solvents are compatible with some bases only as is understood in the art. For example, sodium hydride may preferably be used with dimethylformamide or tetrahydrofuran and sodium hydroxide is preferably used with dichloromethane and a phase transfer catalyst.

The reaction can be carried out at moderate temperatures, for example from 0 to 50° C. and conveniently at about ambient temperature.

Preferably, R^2′ is an ester group in the compound of formula IX and this may be subsequently converted to an acid or to another ester or salt, by conventional methods later in the process. For example, when X is a group SO₂ and R² is a methyl ester of carboxy, it may be converted to the corresponding carboxylic acid by reaction with lithium iodide in dry pyridine or DMF.

Suitable protecting groups R⁴⁰ include acetyl or benzyl. The reaction conditions employed will be variable depending upon the nature of the protecting group R⁴⁰ and would be apparent to a skilled person. Acetyl groups may be removed by reaction with a strong base such as sodium methoxide, whereas benzyl groups may be removed by hydrogenation for example in the presence of a catalyst such as a palladium catalyst.

Compounds of formula (IX) may be prepared by cyclisation of a compound of formula (XII)
where R⁵, R⁶, R⁷ and R⁴⁰ are as defined above and R⁴² and R⁴³ represent a combination of moieties which can cyclise to form an appropriately substituted pyrrole ring. For example, one of R⁴² and R⁴³ can be a group of formula —CH═C(R⁴⁴)N₃ where R⁴⁴ is a group R² as defined above, or a protected form thereof, and the other may be hydrogen. Cyclisation to form a compound of formula (XII) may then be effected by heating for example under reflux in an organic solvent, in particular a high boiling aprotic solvent such as xylene or toluene.

Alternatively, one of R⁴² and R⁴³ may be nitro and the other may be a group of formula —CH₂C(O)R² where R^2′ is as defined above in relation to formula (VII). These compounds will cyclise in the presence of a catalyst such as palladium on carbon in the presence of hydrogen. The reaction may be effected at moderate temperatures for example of from 0 to 80° C., conveniently at about ambient temperature.

Thus examples of compounds of formula (XII) include compounds of formula (XIII) and (XIV)

Compounds of formula (XIII) where R³ is hydrogen may be prepared for example by reacting a compound of formula (XV)
with a compound of formula (XVI)
N₃CH₂R^2′  (XVI)
where R⁵, R⁶, R⁷, and R^2′ are as defined hereinbefore. The reaction may be effected in an organic solvent such as ethanol at low temperatures of from −20 to 0° C., suitably at about 0° C. The reaction is suitably effected in the presence of a base such as an alkoxide, in particular an ethoxide, for example potassium ethoxide.

Compounds of formula (XVI) are suitably prepared by reacting a compound of formula (XVII)
R₄₇CH₂R^2′  (XVII)
where R^2′ is defined above and R⁴⁷ is a leaving group such as halide and in particular bromide, with an azide salt, such as an alkali metal azide salt in particular sodium azide.

Compounds of formula (XIV) may be prepared by reacting a compound of formula (XVIII)
where R⁵, R⁶, R⁷, R³, R⁴⁰ and R^2′ are as defined above, with a compound of formula (XIX)
where R^2′ is as defined above and R⁴⁸ leaving group such as hydroxy. Examples of compounds of formula (XVI) are oxalates such as diethyloxalate. The reaction is suitably effected in the presence of a base such as sodium hydride in an organic solvent such as THF. Moderate temperatures of from 0° to 40° C. and conveniently ambient temperature is employed.

Compounds of formula (VII) are suitably prepared using a reaction analogous to that between compounds (IX) and (X), where in place of the compound of formula (IX), a compound of formula (IXA) is employed
where R^2′, R³, R⁵, R⁶ and R⁷ are as defined above. Such compounds may be obtained by reduction of the corresponding nitro compound of formula (XX)
where R^2′, R³, R⁵, R⁶ and R⁷ are as defined above.

Compounds of formula (X), (XVI), (XV), (XVII), (XVIII), (XIX) and (XX) are either known compounds or they may be prepared from known compounds by conventional literature methods.

According to a further aspect of the invention there is provided a compound of the formula (I) as defined herein, or a pharmaceutically acceptable salt or an in vivo hydrolysable ester thereof, for use in a method of treatment of the human or animal body by therapy. In particular, the compounds are used in methods of treatment of inflammatory disease.

According to a further aspect of the present invention there is provided a method for antagonising an MCP-1 mediated effect in a warm blooded animal, such as man, in need of such treatment, which comprises administering to said animal an effective amount of a compound of formula (I), or a pharmaceutically acceptable salt, or an in vivo hydrolysable ester thereof.

The invention also provides a pharmaceutical composition comprising a compound of formula (I) as defined herein, or a pharmaceutically acceptable salt, or an in vivo hydrolysable ester thereof, in combination with a pharmaceutically acceptable diluent or carrier.

The compositions of the invention may be in a form suitable for oral use (for example as tablets, lozenges, hard or soft capsules, aqueous or oily suspensions, emulsions, dispersible powders or granules, syrups or elixirs), for topical use (for example as creams, ointments, gels, or aqueous or oily solutions or suspensions), for administration by inhalation (for example as a finely divided powder or a liquid aerosol), for administration by insufflation (for example as a finely divided powder) or for parenteral administration (for example as a sterile aqueous or oily solution for intravenous, subcutaneous, intramuscular or intramuscular dosing or as a suppository for rectal dosing).

The compositions of the invention may be obtained by conventional procedures using conventional pharmaceutical excipients, well known in the art. Thus, compositions intended for oral use may contain, for example, one or more colouring, sweetening, flavouring and/or preservative agents.

Suitable pharmaceutically acceptable excipients for a tablet formulation include, for example, inert diluents such as lactose, sodium carbonate, calcium phosphate or calcium carbonate, granulating and disintegrating agents such as corn starch or algenic acid; binding agents such as starch; lubricating agents such as magnesium stearate, stearic acid or talc; preservative agents such as ethyl or propyl p-hydroxybenzoate, and anti-oxidants, such as ascorbic acid. Tablet formulations may be uncoated or coated either to modify their disintegration and the subsequent absorption of the active ingredient within the gastrointestinal track, or to improve their stability and/or appearance, in either case, using conventional coating agents and procedures well known in the art.

Compositions for oral use may be in the form of hard gelatin capsules in which the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules in which the active ingredient is mixed with water or an oil such as peanut oil, liquid paraffin, or olive oil.

Aqueous suspensions generally contain the active ingredient in finely powdered form together with one or more suspending agents, such as sodium carboxymethylcellulose, methylcellulose, hydroxypropylmethylcellulose, sodium alginate, polyvinyl-pyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents such as lecithin or condensation products of an alkylene oxide with fatty acids (for example polyoxyethylene stearate), or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol anhydrides, for example polyethylene sorbitan monooleate. The aqueous suspensions may also contain one or more preservatives (such as ethyl or propyl p-hydroxybenzoate, anti-oxidants (such as ascorbic acid), colouring agents, flavouring agents, and/or sweetening agents (such as sucrose, saccharine or aspartame).

Oily suspensions may be formulated by suspending the active ingredient in a vegetable oil (such as arachis oil, olive oil, sesame oil or coconut oil) or in a mineral oil (such as liquid paraffin). The oily suspensions may also contain a thickening agent such as beeswax, hard paraffin or cetyl alcohol. Sweetening agents such as those set out above, and flavouring agents may be added to provide a palatable oral preparation. These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid.

Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water generally contain the active ingredient together with a dispersing or wetting agent, suspending agent and one or more preservatives. Suitable dispersing or wetting agents and suspending agents are exemplified by those already mentioned above. Additional excipients such as sweetening, flavouring and colouring agents, may also be present.

The pharmaceutical compositions of the invention may also be in the form of oil-in-water emulsions. The oily phase may be a vegetable oil, such as olive oil or arachis oil, or a mineral oil, such as for example liquid paraffin or a mixture of any of these. Suitable emulsifying agents may be, for example, naturally-occurring gums such as gum acacia or gum tragacanth, naturally-occurring phosphatides such as soya bean, lecithin, an esters or partial esters derived from fatty acids and hexitol anhydrides (for example sorbitan monooleate) and condensation products of the said partial esters with ethylene oxide such as polyoxyethylene sorbitan monooleate. The emulsions may also contain sweetening, flavouring and preservative agents.

Syrups and elixirs may be formulated with sweetening agents such as glycerol, propylene glycol, sorbitol, aspartame or sucrose, and may also contain a demulcent, preservative, flavouring and/or colouring agent.

The pharmaceutical compositions may also be in the form of a sterile injectable aqueous or oily suspension, which may be formulated according to known procedures using one or more of the appropriate dispersing or wetting agents and suspending agents, which have been mentioned above. A sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally-acceptable diluent or solvent, for example a solution in 1,3-butanediol.

Suppository formulations may be prepared by mixing the active ingredient with a suitable non-irritating excipient which is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum to release the drug. Suitable excipients include, for example, cocoa butter and polyethylene glycols.

Topical formulations, such as creams, ointments, gels and aqueous or oily solutions or suspensions, may generally be obtained by formulating an active ingredient with a conventional, topically acceptable, vehicle or diluent using conventional procedure well known in the art.

Compositions for administration by insulation may be in the form of a finely divided powder containing particles of average diameter of, for example, 30μ or much less, the powder itself comprising either active ingredient alone or diluted with one or more physiologically acceptable carriers such as lactose. The powder for insufflation is then conveniently retained in a capsule containing, for example, 1 to 50 mg of active ingredient for use with a turbo-inhaler device, such as is used for insufflation of the known agent sodium cromoglycate.

Compositions for administration by inhalation may be in the form of a conventional pressurised aerosol arranged to dispense the active ingredient either as an aerosol containing finely divided solid or liquid droplets. Conventional aerosol propellants such as volatile fluorinated hydrocarbons or hydrocarbons may be used and the aerosol device is conveniently arranged to dispense a metered quantity of active ingredient.

For further information on Formulation the reader is referred to Chapter 25.2 in Volume 5 of Comprehensive Medicinal Chemistry (Corwin Hansch; Chairman of Editorial Board), Pergamon Press 1990.

The amount of active ingredient that is combined with one or more excipients to produce a single dosage form will necessarily vary depending upon the host treated and the particular route of administration. For example, a formulation intended for oral administration to humans will generally contain, for example, from 0.5 mg to 2 g of active agent compounded with an appropriate and convenient amount of excipients which may vary from about 5 to about 98 percent by weight of the total composition. Dosage unit forms will generally contain about 1 mg to about 500 mg of an active ingredient. For further information on Routes of Administration and Dosage Regimes the reader is referred to Chapter 25.3 in Volume 5 of Comprehensive Medicinal Chemistry (Corwin Hansch; Chairman of Editorial Board), Pergamon Press 1990.

The size of the dose for therapeutic or prophylactic purposes of a compound of the Formula I will naturally vary according to the nature and severity of the conditions, the age and sex of the animal or patient and the route of administration, according to well known principles of medicine. As mentioned above, compounds of the Formula I are useful in treating diseases or medical conditions which are due alone or in part to the effects of farnesylation of rats.

In using a compound of the Formula I for therapeutic or prophylactic purposes it will generally be administered so that a daily dose in the range, for example, 0.5 mg to 75 mg per kg body weight is received, given if required in divided doses. In general lower doses will be administered when a parenteral route is employed. Thus, for example, for intravenous administration, a dose in the range, for example, 0.5 mg to 30 mg per kg body weight will generally be used. Similarly, for administration by inhalation, a dose in the range, for example, 0.5 mg to 25 mg per kg body weight will be used. Oral administration is however preferred.

A further aspect of the invention comprises the use of a compound of formula (I) as defined above in the preparation of a medicament for the treatment of inflammatory disease.

The invention is further illustrated, but not limited by the following Examples in which the following general procedures were used unless stated otherwise.

Preparation 1

Ethyl N-(3,4-dichlorobenzyl)-4-nitroindole-2-carboxylate

Ethyl 4-nitroindole-2-carboxylate (26 g) [prepared according to S. M. Parmerter et. al J. Amer. Chem. Soc., 1958, 80, 4621], 3,4-dichlorobenzyl chloride (16 ml), potassium carbonate (17 g) and potassium iodide (2 g) in DMF (250 ml) were stirred at 60° C. for 2 hours. The reaction was concentrated in vacuo and the residue partitioned between water and dichloromethane. Iso-hexane was added to the combined organic extracts resulting in crystallisation of the product as yellow needles (39 g, 89%) NMR d (CD₃SOCD₃) 1.30 (t, 3H), 4.32 (q, 2H), 5.93 (s, 2H), 6.88 (dd, 1H), 7.18 (d, 1H), 7.52 (d, 1H), 7.56 (dd, 1H), 7.78 (s, 1H), 8.17 (m, 2H), M/z (+)395 (MH⁺), 393.

Preparation 2

Ethyl N-benzyl-4-aminoindole-2-carboxylate

A mixture of ethyl 4-nitroindole-2-carboxylate (8.2 g), anhydrous potassium carbonate (6.0 g) and benzyl bromide (4.3 ml) in DMF (100 ml) was stirred at 50-60° C. for 2 hours. The solvent was evaporated in vacuo and the residue partitioned between dichloromethane and water (250 ml each); the organic layer was separated, dried (MgSO₄) and evaporated to give a yellow solid (12 g), which was dissolved in a mixture of tetrahydrofuran/ethanol (200 ml, 1:1) and stirred while adding a solution of sodium dithionite (26 g) in water (50 ml). The mixture was stirred for 1 hour at 25° C. and partitioned between dichloromethane and water (200 ml each), the organic layer was washed with water (100 ml) and dried (MgSO₄). Combined organic extracts were concentrated in vacuo and the residue purified by column chromatography using dichloromethane as eluent to give a the product as a brown solid (1.4 g. 14%); NMR d (CD₃SOCD₃) 1.28 (t, 3H), 4.27 (q, 2H), 5.57 (s, 2H), 5.73 (s, 2H), 6.22 (d, 1H), 6.62 (d, 1H), 6.95-7.05 (m, 3H), 7.15-7.30 (m, 3H), 7.60 (s, 1H).

Preparation 3

Ethyl N-(3,4-dichlorobenzyl)-4-nitroindole-2-carboxylate

Sodium hydroxide (3M, 20 ml) was added to a vigorously stirred solution of ethyl 4-nitroindole-2-carboxylate (4 g), 3,4-dichlorobenzyl chloride (4.73 ml) and tetra-n-butylammonium hydrogensulphate (0.2 g) in dichloromethane (60 ml). The reaction was stirred for 48 hours then partitioned between 2M HCl and dichloromethane. Combined organic extracts were dried (MgSO₄) and concentrated in vacuo and the residue purified by column chromatography using iso-hexane: 20% ethyl acetate as eluent to give the product as a yellow crystalline solid (5.26 g, 78%); NMR δ (CD₃SOCD₃) 1.3 (t, 3H), 4.3 (q, 2H), 5.95 (s, 2H), 6.9 (m, 1H), 7.6 (m, 4H), 8.2 (t, 2H); M/z (+) 393.3 (M⁺).

Ethyl N-(3,4-dichlorobenzyl)-4-aminoindole-2-carboxylate

A solution of ethyl N-(3,4-dichlorobenzyl)-4-nitroindole-2-carboxylate (2.41 g) in tetrahydrofuran (100 ml) was stirred in the presence of titanium trichloride (1.5% aqueous solution. 50 ml) at room temperature overnight. The reaction was treated with 40% sodium hydroxide solution and extracted with 5% methanol in dichloromethane. Combined organic extracts were dried (MgSO₄) and concentrated in vacuo to Live the product as a brown solid (1.98 g, 89%); NMR d (CD₃SOCD₃) 1.3 (t, 3H), 4.2 (q, 2H), 5.7 (s, 4H), 6.2 (d, 1H), 6.6 (d, 1H), 7.0 (m, 2H), 7.25 (m, 1H), 7.5 (d, 1H), 7.6 (m, 1H); M/z (+) 363.3 (MH⁺).

Preparation 4

Ethyl 4-chloroacetamido-N-(3,4-dichlorobenzyl)indole-2-carboxylate

Ethyl 4-amino-N-(3,4-dichlorobenzyl)indole-2-carboxylate (2.03 g), chloroacetyl chloride (0.5 ml) and triethylamine (4.0 ml) were stirred in dichloromethane (50 ml) for 16 hours. The reaction was washed with water, dried (MgSO₄) and concentrated in vacuo. The residue was triturated with toluene to give the product as a pale grey solid (1.61 g, 65%); NMR d (CD₃SOCD₃) 1.28 (t, 3H), 4.30 (q, 2H), 4.40 (s, 2H), 5.81 (s, 2H), 6.88 (dd, 1H), 7.30 (m, 3H), 7.50 (d, 1H), 7.76 (s, 1H), 7.78 (d, 1H), 10.19 (brs, 1H); M/z (−) 439 (M⁺), 437.

EXAMPLE 1

Compound 2

Ethyl 4-chloroacetamido-N-(3,4-dichlorobenzyl)indole-2-carboxylate (0.15 g) and morpholine (2.0 ml) were dissolved in methoxyethanol (5.0 ml) and the reaction stirred for 72 hours. The reaction was then poured into water (100 ml) and the resulting solid filtered and dried in vacuo. The solid was dissolved in THF (2.5 ml) and methanol (2.5 ml), and to this was added NaOH (3M, 2.0 ml). The reaction was stirred for 16 hours, then concentrated. The residue was dissolved in water, and precipitated by dropwise addition of acetic acid. The resulting solid was filtered and dried in vacuo to give the title compound as a white solid (0.1 g, 63%, 2 steps); NMR d (CD₃SOCD₃) 2.58 (t, 4H), 3.29 (s, 2H), 3.65 (t, 4H), 5.82 (s, 2H), 6.90 (dd, 1H), 7.30 (m, 3H), 7.52 (m, 2H), 7.72 (d, 1H), 9.80 (s, 1H); M/z (−) 462 (M⁺), 460, 418.

EXAMPLE 2

The procedure described in Example 1 above was repeated using the appropriate amines. Thus were obtained the compounds described below.

Compound 3

49% yield, 2 steps; NMR d (CD₃SOCD₃) 2.27 (s, 3H), 2.54 (t, 4H), 2.62 (t, 4H), 3.22 (s, 2H), 5.84 (s, 2H), 6.95 (dd, 1H), 7.22 (m, 2H), 7.33 (s, 1H), 7.41 (s, 1H), 7.50 (d, 1H), 7.72 (d, 1H), 9.75 (s, 1H); M/z (−) 475 (M⁺), 473, 429, 109.

Compound 6

14% yield, 2 steps; M/z (−) 510 (M⁻), 508, 464.

EXAMPLE 3

Di-ester of Compound 8

Ethyl 4-chloroacetamido-N-(3,4-dichlorobenzyl)indole-2-carboxylate (0.4 g), glycine methyl ester hydrochloride (0.57 g) and triethylamine (1.25 ml) were dissolved in methoxyethanol (4.0 ml) and the reaction heated at 100° C. for 6 hours. The reaction was cooled and partitioned between water and ethyl acetate. Combined organic extracts were dried (MgSO₄) and concentrated and the residue purified by chromatography using toluene ethyl acetate (1:1) as eluent to give the product, ethyl 4-[(N-(methoxycarbonylmethyl)-glycyl)amino]-N-(3,4-dichlorobenzyl)indole-2-carboxylate, as a pale yellow solid (0.17 g, 38%); NMR d (CD₃SOCD₃) 1.28 (t, 3H), 3.44 (s, 2H), 3.50 (s, 2H), 3.63 (s, 3H), 4.28 (q, 2H), 5.82 (s, 2H), 6.88 (dd, 1H), 7.10-7.30 (m, 4H), 7.50 (d, 1H), 7.69 (s, 1H), 7.80 (dd, 1H), 10.00 (brs, 1H); M/z (+) 494, 492 (M⁺).

EXAMPLE 4

Di-ester of Compound 11

Methanesulphonyl chloride (0.1 ml) was added to stirred solution of ethyl 4-[(N-(methoxycarbonylmethyl)glycyl)amino]-N-(3,4-dichlorobenzyl)indole-2-carboxylate (0.33 g) and triethylamine (0.47 ml) in dichloromethane (4.0 ml) and the reaction stirred for 3 hours. The reaction was poured into water and extracted with ethyl acetate. Combined organic extracts were dried (MgSO₄) and concentrated and the residue triturated with ether, filtered and dried in vacuo to give the product as a white solid (0.24 g, 63%); NMR d (CD₃SOCD₃) 1.27 (t, 3H), 3.10 (s, 3 H), 3.67 (s, 3H), 4.20 (s, 2H), 4.28 (q+s, 2H+2H), 5.82 (s, 2H), 6.87 (dd, 1H), 7.28 (m, 3H), 7.50 (d, 1H), 7.80 (m, 2H), 10.00 (brs, 1H); M/z (+) 572, 570 (M⁺).

EXAMPLE 5

The procedure described in the Example 4 above was repeated using the appropriate acid chloride. Thus was obtained the compound described below.

Di-ester of Compound 12

64% yield; M/z (−) 534 (M⁺), 532.

EXAMPLE 6

Di-ester of Compound 14

Sarcosine ethyl ester hydrochloride (1.23 g) and potassium carbonate (1.11 g) were added to a solution of ethyl 4-chloroacetamido-N-(3,4-dichlorobenzyl)indole-2-carboxylate (700 mg) in acetone (25 ml), stirred and heated at 65° C. overnight. The reaction was partitioned between water (50 ml) and ethyl acetate (50 ml), extracted with ethyl acetate (2×50 ml), and dried (MgSO₄). The combined organic extracts were concentrated in vacuo, and the residue purified by column chromatography using 30% ethyl acetate:toluene as eluent, to afford the product as a yellow solid (768 mg, 92%); NMR d (CD₃SOCD₃) 1.21 (t, 3H), 1.28 (t, 3H), 2.45 (s, 3H), 3.42 (s, 2H), 3.53 (s, 2H), 4.16 (q, 2H), 4.30 (q, 2H), 5.81 (s, 2H), 6.88 (d, 1H), 7.27 (m, 2H), 7.52 (d, 1H), 7.67 (s, 1H), 7.84 (d, 1H), 9.95 (s, 1H), M/z (+) 520.3 (MH⁺)

EXAMPLE 7

The procedure described in Example 6 above was repeated using the appropriate amine. Thus was obtained the compound described below.

Diester of Compound 13

93% yield; NMR d (CD₃SOCD₃) 1.15 (t, 3H), 1.28 (t, 3H), 3.52 (s, 3H), 3.57 (s, 3H), 3.87 (s, 2H), 4.10 (q, 2H), 4.31 (q, 2H),5.83 (s, 2H), 6.90 (d, 1H), 7.15-7.44 (m, 8H), 7.53 (d, 1H), 7.67 (s, 1H), 7.83 (d, 1H); M/z (+) 596.5 (MH⁺).

EXAMPLE 8

Di-ester of Compound 15

A solution of methyl iodide (0.026 ml) in DMF (2 ml) was added to a solution of sodium hydride (15 mg, 60% in mineral oil) and ethyl 4-[(N-benzyl-N-ethoxycarbonylmethyl)glycyl]amino-N-(3,4-dichlorobenzyl)indole-2-carboxylate (the diester of Compound 13) ( 200 mg) in DMF (4 ml), and stirred under an atmosphere of argon at ambient temperature for 4 hours. The reaction was quenched with water (50 ml) and extracted with ethyl acetate (3×50 ml), and the combined organic extracts were dried (MgSO₄), and concentrated in vacuo to afford the product as a pale brown oil (93 mg, 45%); NMR d (CD₃SOCD₃) 1.05 (t, 3H), 1.30 (t, 3H), 3.21 (s, 2H), 3.28 (s, 3H), 3.41 (s, 2H), 3.70 (s, 2H), 3.93 (q, 2H), 4.30 (q, 2H), 5.84 (s, 2H), 6.90 (d, 1H), 7.01 (d, 1H), 7.07-7.40 (m, 8H), 7.48-7.64 (m, 2H); M/z (+) 610.5 (MH⁺).

EXAMPLE 9

Compound 8

Ethyl 4-[(N-(methoxycarbonylmethyl)glycyl)amino]-N-(3,4-dichlorobenzyl)indole-2-carboxylate (0.15 g) was dissolved in THF/methanol (1:1) (10 ml) and sodium hydroxide (2M, 2.5 ml) was added and the reaction stirred for 16 hours. The reaction was then concentrated in vacuo and the residue dissolved in water. The solution was acidified by dropwise addition of acetic acid, resulting in the precipitation of a white solid which was filtered, washed with water and dried in vacuo to give the desired end product as a white solid (108 mg, 79%); NMR d (CD₃SOCD₃) 3.40 (s, 2H), 3.64 (s, 2H), 5.82 (s, 2H), 6.92 (dd, 1H), 7.20-7.38 (m, 3H), 7.50 (d, 1H), 7.62 (s, 1H), 7.78 (d, 1H), 10.15 (brs, 1H).

EXAMPLE 10

The procedure described in Example 9 above was repeated using the appropriate ester. Thus were obtained the compounds described below.

Compound 11

79% yield; NMR d (CD₃SOCD₃) 3.10 (s, 3H), 4.02 (s, 2H), 4.20 (s, 2H), 5.83 (s, 2H), 6.88 (dd, 1H), 7.25 (m, 3H), 7.50 (d, 1H), 7.75 (s, 1H), 7.80 (d, 1H), 10.49 (brs, 1H); M/z (−) 528 (M⁺), 526, 360, 358, 289, 253, 217.

Compound 12

78% yield; NMR d (CD₃SOCD₃) 2.00 (d, 3H), 4.03 (s, 1H), 4.20 (s, 1H), 4.23 (s, 1H), 4.40 (s, 1H), 5.82 (s, 2H), 6.88 (m, 1H), 7.25 (m, 3H), 7.52 (dd, 1H), 7.76 (m, 2H), 10.13 (brs, 1H); M/z (−) 492 (M⁺), 490, 324, 253, 224.

Compound 14

60% yield; NMR d (CD₃SOCD₃) 2.46 (s, 31H), 3.38 (s, 2H), 3.42 (s, 2H), 5.88 (s, 2H), 6.92 (d, 1H), 7.20 (m, 2H), 7.31 (s, 1H), 7.50 (m, 2H), 7.82 (d, 1H), M/z (−) 462.2 (M−H⁺).

Compound 15

15% yield; NMR d (CD₃SOCD₃) 3.21 (s, 2H), 3.31 (s, 3H), 3.40 (s, 2H), 3.69 (s, 2H), 5.83 (s, 2H), 6.90 (d, 2H), 6.98 (d, 2H), 7.15 (m, 6H), 7.27 (t, 1H), 7.39 (s, 1H), 7.53 (m, 2H); M/z (−) 554.3 (M−H⁺).

Compound 13

25% yield; NMR d (CD₃SOCD₃) 3.44 (s, 2H), 3.46 (s, 2H), 3.85 (s, 2H), 5.91 (s, 2H), 6.87 (m, 1H), 7.13-7.36 (m, 6H), 7.40 (m, 2H), 7.53 (m, 2H), 7.78 (d, 1H), M/z (−) 538.2 (M−H⁺), 253.2.

EXAMPLE 11

N-Benzyl-4-(2-(pyrid-2-yl)thiophene-5-sulphonyl))aminoindole-2-carboxylic Acid (Compound 1)

To a solution of ethyl N-benzyl-4-aminoindole-2-carboxylate (140 mg) and pyridine (0.08 ml) in dichloromethane (10 ml) at 20° C. was added 2-(pyrid-2-yl)thiophene-5-sulphonyl chloride (140 mg) and thc reaction stirred for 2 hours. The mixture was washed with HCl (2M, 10 ml), the organic layer was concentrated in vacuo and the residue purified by chromatography on silica using ethyl acetate as eluent, to give a yellow solid which was dissolved in ethanol (50 ml) at 60° C. and treated with NaOH (2M, 4.0 ml) with stirring for 2 hours. The solvent was evaporated in vacuo, the residue dissolved in water (50 ml) and filtered. The clear yellow filtrate was acidified with 2N HCl and extracted with dichloromethane/methanol (9:1, 100 ml). The organic layer was dried (MgSO₄) and evaporated to give a pale brown solid, which was triturated with ether to give the product as an off white powder (150 mg, 63%, 2 steps); NMR d (CD₃SOCD₃) 5.87 (s, 2H), 6.9-7.1 (m, 9H), 7.30 (dd, 2H), 7.43 (d, 1H), 7.63 (d, 1H), 7.81 (dd, 1H), 7.96 (d, 1H), 8.50 (d, 1H); M/z (−) 488 (M−H⁺).

EXAMPLE 12

The procedure described in Example 11 above was repeated using the appropriate aminoindole and sulphonyl chloride. Thus were obtained the compounds described below.

4-(4-Acetylaminobenzenesulphonyl)amino-N-(3,4-dichlorobenzyl)indole-2-carboxylic Acid (Compound 4)

66% yield (2 steps); NMR d (CD₃SOCD₃) 2.00 (s, 3H), 5.75 (s, 2H), 6.80 (dd, 1H), 6.92 (d, 1H), 7.12 (dd, 1H), 7.22 (m, 2H), 7.48 (d, 1H), 7.56 (s, 1H,), 7.66 (s, 4H), 10.24 (brs, 1H), 10.45 (brs, 1H); M/z (−) 532 (M−H⁺), 530.

N-(3,4-Dichlorobenzyl)-4-(2-(pyrid-2-yl)thiophene-5-sulphonyl))aminoindole-2-carboxylic Acid (Compound 5)

69% yield (2 steps); NMR d (CD₃SOCD₃) 5.80 (s, 2H), 6.80 (dd, 1H), 7.0-7.5 (m, 8H), 7.68 (d, 1H), 7.83 (dd, 1H), 7.92 (d, 1H), 8.48 (dd, 1H); M/z (−) 558 (M−H⁺), 556.

N-(3,4-Dichlorobenzyl)-4-(1-methylimidazole-4-sulphonyl)aminoindole-2-carboxylic Acid (Compound 7)

66% yield (2 steps); NMR d (CD₃SOCD₃) 3.60 (s, 3H)) 5.78 (s, 2H), 6.86 (dd, 1H),7.04(1H, d), 7.15 (dd, 1H), 7.20 (d, 1H), 7.30 (d, 1H) 7.50 (d, 1H), 7.68 (m, 2H), 7.75 (s, 1H), 10.20 (brs, 1H); M/z (−) 479 (M−H⁺), 477.

N-(3,4-Dichlorobenzyl)-4-(2-chloropyridyl-5-sulphonyl)aminoindole-2carboxylic Acid

(Compound 9)

30% yield (2 steps); NMR d (CD₃SOCD₃) 5.85 (s, 2H), 6.83 (d, 1H), 6.93 (dd, 1H), 7.03 (dd, 1H), 7.15 (d, 1H), 7.20 (s, 1H), 7.26 (s, 1H), 7.46 (d, 1H), 7.60 (d, 1H), 8.05 (dd, 1H), 8.62 (d, 1H); M/z (−)512(M−H⁺),510, 508.

EXAMPLE 13

Methyl N-(3,4-dichlorobenzyl)-4-(dimethylcarbamyloxy)indole-2-carboxylate (Methyl Ester of Compound 10)

Dimethylcarbamyl chloride (83 mg) was added to a stirred solution of methyl N-(3,4-dichlorobenzyl)-4-hydroxyindole-2-carboxylate (150 mg), triethylamine (65 mg),and DMAP (5 mg) in dichloromethane. The reaction was stirred for 16 hours at room temperature under an atmosphere of nitrogen. The reaction was washed with hydrochloric acid (2M, 70 ml), saturated aqueous sodium hydrogencarbonate solution, water and saturated sodium chloride solution. Combined organic extracts were dried (MgSO₄), concentrated in vacuo and the residue purified by column chromatography using 60% ethyl acetate:iso-hexane as eluent to give the product as a colourless gum (132 mg, 74%); NMR d (CD₃SOCD₃) 2.94 (s, 3H), 3.12 (s, 3H), 3.81 (s, 3H), 5.82 (s, 2H), 6.91 (m, 2H), 7.21 (s, 1H), 7.27-7.36 (m, 2H), 7.46 (d, 1H), 7.52 (d, 1H); M/z (+) 421 (MH⁺).

EXAMPLE 14

N-(3,4-Dichlorobenzyl)-4-(dimethylcarbamyloxy)indole-2-carboxylic Acid (Compound 10)

Desesterifiation of the compound of Example 13 using the method described in Example 9 above yielded Compound 10.

93% yield; NMR d (CD₃SOCD₃) 2.94 (s, 3H), 3.11 (s, 3H), 5.91 (s, 2H), 6.82 (d, 1H), 6.94-7.03 (m, 2H), 7.18 (t, 1H), 7.29-7.39 (m, 2H), 7.50 (d, 1H); M/z (−) 405 (M−H⁺).

EXAMPLE 15

Biological Assays for hMCP-1 Antagonists

The following biological test methods, data and Examples serve to illustrate the present invention.

Abbreviations:

• ATCC American Type Culture Collection, Rockville, USA.
• BCA Bicinchroninic acid, (used, with copper sulphate, to assay protein)
• BSA Bovine Serum Albumin
• DMEM Dulbecco's modified Eagle's medium
• EGTA Ethylenebis(oxyethylenenitrilo)tetraacetic acid
• FCS Foetal calf serum
• HEPES (N-[2-Hydroxyethyl]piperazine-N′-[2-ethanesulphonic acid])
• HBSS Hank's Balanced Salt Solution
• hMCP-1 Human Monocyte Chemoattractant Protein-1
• PBS Phosphate buffered saline
• PCR Polymerase chain reaction

AMPLITAQ™, available from Perkin-Elmer Cetus, is used as the source of thermostable DNA polymerase.

Binding Buffer is 50 mM HEPES, 1 mM CaCl₂, 5 mM MgCl₂, 0.5% foetal calf serum, adjusted to pH 7.2 with 1 M NaOH.

Non-Essential Amino Acids (100× concentrate) is: L-Alanine, 890 mg/l; L-Asparagine, 1320 mg/l; L-Aspartic acid, 1330 mg/l; L-Glutamic acid, 1470 mg/l; Glycine, 750 mg/l; L-Proline, 1150 mg/l and; L-Serine, 1050 mg/l.

Hypoxanthinc and Thymidine Supplement (50× concentrate) is: hypoxanthine, 680 mg/l and; thymidine, 194 mg/l.

Penicillin-Streptomycin is: Penicillin G (sodium salt); 5000 units/ml. Streptomycin sulphate, 5000 μg/ml.

Human monocytic cell line THP-1 cells are available from ATCC, accession number ATCC TIB-202.

Hank's Balanced Salt Solution (HBSS) was obtained from Gibco; see Proc. Soc. Exp. Biol. Med., 1949, 71, 196.

Synthetic cell culture medium, RPMI 1640 was obtained from Gibco; it contains inorganic salts [Ca(NO₃)₂.4H₂O 100 mg/l; KCl 400 mg/l; MgSO₁.7H₂O 100 mg/l; NaCl 6000 mg/l; NaHCO₃ 2000 mg/l & Na₂HPO₄ (anhyd) 800 mg/l], D-Glucose 2000 mg/l, reduced glutathione 1 mg/l, amino acids and vitamins.

FURA-2/AM is 1-[2-(5-carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2′-amino-5′-methylphenoxy)-ethane-N,N,N′,N′-tetraacetic acid pentaacetoxymethyl ester and was obtained from Molecular Probes, Eugene, Oreg., USA.

Blood Sedimentation Buffer contains 8.5 g/l NaCl and 10 g/l hydroxyethyl cellulose.

Lysis Buffer is 0.15M NH₄Cl⁻, 10 mM KHCO₃, 1 mM EDTA

Whole Cell Binding Buffer is 50 mM HEPES, 1 mM CaCl₂, 5 mM MgCl₂, 0.5% BSA, 0.01% NaN₃, adjusted to pH 7.2 with 1M NaOH.

Wash buffer is 50 mM HEPES, 1 mM CaCl₂, 5 mM MgCl₂, 0.5% heat inactivated FCS, 0.5M NaCl adjusted to pH7.2 with 1M NaOH.

General molecular biology procedures can be followed from any of the methods described in “Molecular Cloning—A Laboratory Manual” Second Edition, Sambrook, Fritsch and Maniatis (Cold Spring Harbor Laboratory, 1989).

i) Cloning and Expression of hMCP-1 Receptor

The MCP-1 receptor B (CCR2B) cDNA was cloned by PCR from THP-1 cell RNA using suitable oligonucleotide primers based on the published MCP-1 receptor sequences (Charo et al., 1994, Proc. Natl. Acad. Sci. USA, 91, 2752). The resulting PCR products were cloned into vector PCR-II™ (InVitrogen, San Diego, Calif.). Error free CCR2B cDNA was subcloned as a Hind III-Not I fragment into the eukaryotic expression vector pCDNA3 (InVitrogen) to generate pCDNA3/CC-CKR2A and pCDNA3/CCR2B respectively.

Linearised pCDNA3/CCR2B DNA was transfected into CHO-K1 cells by calcium phosphate precipitation (Wigler et al., 1979, Cell, 16, 777). Transfected cells were selected by the addition of Geneticin Sulphate (G418, Gibco BRL) at 1 mg/ml, 24 hours after the cells had been transfected. Preparation of RNA and Northern blotting were carried out as described previously (Needham et al, 1995, Prot. Express. Purific., 6, 134). CHO-K1 clone 7 (CHO-CCR2B) was identified as the highest MCP-1 receptor B expressor.

ii) Preparation of Membrane Fragments

CHO-CCR2B cells were grown in DMEM supplemented with 10% foetal calf serum. 2 mM glutamine, 1× Non-Essential Amino Acids, 1× Hypoxanthine and Thymidine Supplement and Penicillin-Streptomycin (at 50 μg streptomycin/ml. Gibco BRL). Membrane fragments were prepared using cell lysis/differential centrifugation methods as described previously (Siciliano et al., 1990, J. Biol. Chem., 265, 19658). Protein concentration was estimated by BCA protein assay (Pierce, Rockford, Ill.) according to the manufacturer's instructions.

iii) Assay

¹²⁵I MCP-1 was prepared using Bolton and Hunter conjugation (Bolton et al., 1973, Biochem. J., 133, 529; Amersham International plc]. Equilibrium binding assays were carried out using the method of Ernst et al., 1994, J. Immunol., 152, 3541. Briefly, varying amounts of ¹²⁵I-labeled MCP-1 were added to 7 μg of purified CHO-CCR2B cell membranes in 100 μl of Binding Buffer. After 1 hour incubation at room temperature the binding reaction mixtures were filtered and washed 5 times through a plate washer (Brandel MLR-96T Cell Harvester) using ice cold Binding Buffer. Filter mats (Brandel GF/B) were pre-soaked for 60 minutes in 0.3% polyethylenimine prior to use. Following filtration individual filters were separated into 3.5 ml tubes (Sarstedt No. 55.484) and bound ¹²⁵I-labeled MCP-1 was determined (LKB 1277 Gammamaster). Cold competition studies were performed as above using 100 pM ¹²⁵I-labeled MCP-1 in the presence of varying concentrations of unlabelled MCP-1. Non-specific binding was determined by the inclusion of a 200-fold molar excess of unlabelled MCP-1 in the reaction.

Ligand binding studies with membrane fragments prepared from CHO-CCR2B cells showed that the CCR2B receptor was present at a concentration of 0.2 pmoles/mg of membrane protein and bound MCP-1 selectively and with high affinity (IC₅₀=110 pM, K_d=120 pM). Binding to these membranes was completely reversible and reached equilibrium after 45 minutes at room temperature, and there was a linear relationship between MCP-1 binding and CHO-CCR2B cell membrane concentration when using MCP-1 at concentrations between 100 pM and 500 pM.

Test compounds dissolved in DMSO (5 μl) were tested in competition with 100 pM labelled MCP-1 over a concentration range (0.01-50 μM) in duplicate using eight point dose-response curves and IC₅₀ concentrations were calculated.

Compounds tested of the present invention had IC₅₀ values of 50 μM or less in the hMCP-1 receptor binding assay described herein. For example Compound 2 in Table 1 showed IC₅₀ of 1.17 μM in hMCP-1.

b) MCP-1 Mediated Calcium Flux in THP-1 Cells

The human monocytic cell line THP-1 was grown in a synthetic cell culture medium RPMI 1640 supplemented with 10% foetal calf serum, 6 mM glutamine and Penicillin-Streptomycin (at 50 μg streptomycin/ml, Gibco BRL). THP-1 cells were washed in HBSS (lacking Ca²⁺ and Mg²⁺)+1 mg/ml BSA and resuspended in the same buffer at a density of 3×10⁶ cells/ml. The cells were then loaded with 1 mM FURA-2/AM for 30 min at 37° C., washed twice in HBSS, and resuspended at 1×10⁶ cells/ml. THP-1 cell suspension (0.9 ml) was added to a 5 ml disposable cuvette containing a magnetic stirrer bar and 2.1 ml of prewarmed (37° C.) HBSS containing 1 mg/ml BSA, 1 mM MgCl₂ and 2 mM CaCl₂. The cuvette was placed in a fluorescence spectrophotometer (Perkin Elmer, Norwalk, Conn.) and preincubated for 4 min at 37° C. with stirring. Fluorescence was recorded over 70 sec and cells were stimulated by addition of hMCP-1 to the cuvette after 10 sec. [Ca²⁺]i was measured by excitation at 340 nm and 380 nm alternately and subsequent measurement of the intensity of the fluorescence emission at 510 nm. The ratio of the intensities of the emitted fluorescent light following excitation at 340 nm and 380 nm, (R), was calculated and displayed to give and estimate of cytoplasmic [Ca²⁺] according to the equation:- $\left[{\mathrm{Ca}}^{2+}\right]i=K_d\frac{\left(R-R\min \right)}{\left(R\max -R\right)}\left(\mathrm{Sf2}/\mathrm{Sb2}\right)$
where the K_d for FURA-2 Ca²⁺ complex at 37° C. was taken to be 224 nm. R_max is the maximal fluorescence ratio determined after addition of 10 mM lonomycin, R_min is the minimal ratio determined by the subsequent addition of a Ca²⁺ free solution containing 5 mM EGTA, and Sf2/Sb2 is the ratio of fluorescence values at 380 nm excitation determined at R_min and R_max, respectively.

Stimulation of THP-1 cells with hMCP-1 induced a rapid, transient rise in [Ca ²⁺]_i in a specific and dose dependent manner. Dose response curves indicated an approximate EC₅₀ of 2 nm. Test compounds dissolved in DMSO (10 μl) were assayed for inhibition of calcium release by adding them to the cell suspension 10 sec prior to ligand addition and measuring the reduction in the transient rise in [Ca²⁺]i. Test compounds were also checked for lack of agonist activity by addition in place of hMCP-1.

c) hMCP-1 and RANTES Mediated Chemotaxis.

In vitro chemotaxis assays were performed using the human monocytic cell line THP-1. Cell migration through polycarbonate membranes was measured by enumerating those passing through either directly by Coulter counting or indirectly by use of a colourimetric viability assay measuring the cleavage of a tetrazolium salt by the mitochondrial respiratory chain (Scudiero D. A. et al. 1988, Cancer Res., 48, 4827-4833).

Chemoattractants were introduced into a 96-well microtitre plate which forms the lower well of a chemotaxis chamber fitted with a PVP-free 5 μm poresize polycarbonate adhesive framed filter membrane (NeuroProbe MB series, Cabin John, Md. 20818, USA) according to the manufacturer's instructions. The chemoattractant was diluted as appropriate in synthetic cell culture medium, RPMI 1640 (Gibco) or supplemented with 2 mM glutamine and 0.5% BSA, or alternatively with HBSS with Ca²⁻ and Mg²⁺ without Phenol Red (Gibco) plus 0.1% BSA. Each dilution was degassed under vacuum for 30 min and was placed (400 μl) in the lower wells of the chamber and THP-1 cells (5×10⁵ in 100 μl RPMI 1640+0.5% BSA) were incubated in each well of the upper chamber. For the inhibition of chemotaxis the chemoattractant was kept at a constant submaximal concentration determined previously (1 nM MCP-1) and added to the lower well together with the test compounds dissolved in DMSO (final DMSO concentration <0.05% v/v) at varying concentrations. The chamber was incubated for 2 h at 37° C. under 5% CO₂. The medium was removed from the upper wells which were then washed out with 200 μl physiological saline before opening the chamber, wiping dry the membrane surface and centrifuging the 96-well plate at 600 g for 5 min to harvest the cells. Supernatant (150 μl) was aspirated and 10 μl of cell proliferation reagent, WST-1, {4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-phenyl disulfonate} plus an electron coupling reagent (Boehringer Mannheim, Cat. no. 1644 807) was added back to the wells. The plate was incubated at 37° C. for 3 h and the absorbance of the soluble formazan product was read on a microtitre plate reader at 450 nm. The data was input into a spreadsheet, corrected for any random migration in the absence of chemoattractant and the average absorbance values, standard error of the mean, and significance tests were calculated. hMCP-1 induced concentration dependent cell migration with a characteristic biphasic response, maximal 0.5-1.0 nm.

In an alternative form of the above assay, fluorescently tagged cells can be used in order to assist in end point detection. In this case, the THP-1 cells used are fluorescently tagged by incubation in the presence of 5 mM Calcein AM (Glycine. N,N′-[[3′,6′-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-2′,7′-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]]-bis[(acetyloxy)methyl]ester: Molecular Probes) for 45 minutes in the dark. Cells are harvested by centrifugation and resuspended in HBSS (without Phenol Red) with Ca²⁺, Mg²⁺ and 0.1% BSA. 50 μl (2×105 cells) of the cell suspension are placed on the filter above each well and, as above, the unit is incubated at 37° C. for 2 hours under 5% CO₂. At the end of the incubation, cells are washed off the upper face of the filter with phosphate buffered saline, the filter removed from the plate and the number of cells attracted to either the underside of the filter or the lower well estimated by reading fluorescence at 485 nm excitation, 538 nm emission wavelengths (fmax, Molecular Devices). The data was input into a spreadsheet, corrected for any random migration in the absence of chemoattractant and the average fluorescence values, standard error of the mean, percentage inhibition and IC₅₀ of compounds under test and significance tests can be calculated. In addition to MCP-1 induced chemotaxis, this alternative form of the assay was also used to measure inhibition of RANTES (2 nM) induced chemotaxis.

d) Binding to Human Peripheral Blood Mononuclear Cells (PBMCs)

i) Preparation of Human PBMCs

Fresh human blood (200 ml) was obtained from volunteer donors, collected into sodium citrate anticoagulant to give a final concentration of 0.38%. The blood was mixed with Sedimentation Buffer and incubated at 37° C. for 20 minutes. The supernatant was collected and centrifuged at 1700 rpm for 5 minutes (Sorvall RT6000D). The pellet obtained was resuspended in 20 ml RPMI/BSA (1 mg/ml) and 4×5 mls of cells were carefully layered over 4×5 mls of Lymphoprepä (Nycomed) in 15 ml centrifuge tubes. Tubes were spun at 1700 rpm for 30 minutes (Sorvall RT6000D) and the resultant layer of cells was removed and transferred to 50 ml Falcon tubes. The cells were washed twice in Lysis Buffer to remove any remaining red blood cells followed by 2 washes in RPMI/BSA. Cells were resuspended in 5 mls of Binding Buffer. Cell number was measured on a Coulter counter and additional binding buffer was added to give a final concentration of 1.25×10⁷ PBMCs /ml.

ii) Assay

[¹²⁵I]MCP-1 was prepared using Bolton and Hunter conjugation (Bolton et al. 1973, Biochem. J., 133, 529; Amersham International plc]. Equilibrium binding assays were carried out using the method of Ernst et al., 1994, J. Immunol., 152, 3541. Briefly, 50 μl of ¹²⁵I-labeled MCP-1 (final concentration 100 pM) was added to 40 μl (5×10⁵ cells) of cell suspension in a 96 well plate. Compounds. diluted in Whole Cell Binding Buffer from a stock solution of 10 mM in DMSO were added in a final volume of 5 μl to maintain a constant DMSO concentration in the assay of 5%. Total binding was determined in the absence of compound. Non-specific binding was defined by the addition of 5 μl cold MCP-1 to give a final assay concentration of 100 nM. Assay wells were made up to a final volume of 100 μl with Whole Cell Binding Buffer and the plates sealed. Following incubation at 37° C. for 60 minutes the binding reaction mixtures were filtered and washed for 10 seconds using ice cold Wash Buffer using a plate washer (Brandel MLR-96T Cell Harvester). Filter mats (Brandel GF/B) were pre-soaked for 60 minutes in 0.3% polyethylenimine plus 0.2% BSA prior to use. Following filtration individual filters were separated into 3.5 ml tubes (Sarstedt No. 55.484) and bound ¹²⁵I-labeled MCP-1 was determined (LKB 1277 Gammamaster).

Test compound potency was determined by assay in duplicate using six point dose-response curves and IC₅₀ concentrations were determined.

Compound No. 13 in Table 1 showed 94% inhibition at 20 μm.

No physiologically unacceptable toxicity was observed at the effective dose for compounds tested of the present invention.

EXAMPLE 16

Pharmaceutical Compositions

The following Example illustrates, but is not intended to limit, pharmaceutical dosage forms of the invention as defined herein (the active ingredient being termed “Compound X”), for therapeutic or prophylactic use in humans:

(a)
Tablet I                         mg/tablet
Compound X.                      100
Lactose Ph. Eur                  182.75
Croscarmellose sodium            12.0
Maize starch paste (5% w/v paste) 2.25
Magnesium stearate               3.0
(b)
Tablet II                        mg/tablet
Compound X                       50
Lactose Ph. Eur                  223.75
Croscarmellose sodium            6.0
Maize starch                     15.0
Polyvinylpyrrolidone (5% w/v paste) 2.25
Magnesium stearate               3.0
(c)
Tablet III                       mg/tablet
Compound X                       1.0
Lactose Ph. Eur                  93.25
Croscarmellose sodium            4.0
Maize starch paste (5% w/v paste) 0.75
Magnesium stearate               1.0
(d)
Capsule                          mg/capsule
Compound X                       10
Lactose Ph. Eur                  488.5
Magnesium                        1.5
(e)
Injection I                      (50 mg/ml)
Compound X                       5.0% w/v
1M Sodium hydroxide solution     15.0% v/v
0.1M Hydrochloric acid           to adjust pH to 7.6
Polyethylene glycol 400          4.5% w/v
Water for injection              to 100%
(f)
Injection II                     (10 mg/ml)
Compound X                       1.0% w/v
Sodium phosphate BP              3.6% w/v
0.1M Sodium hydroxide solution   15.0% v/v
Water for injection              to 100%
(g)
Injection III                    (1 mg/ml. buffered to pH6)
Compound X                       0.1% w/v
Sodium phosphate BP              2.26% w/v
Citric acid                      0.38% wlv
Polyethylene glycol 400          3.5% w/v
Water for injection              to 100%
(h)
Aerosol I                        mg/ml
Compound X                       10.0
Sorbitan trioleate               13.5
Trichlorofluoromethane           910.0
Dichlorodifluoromethane          490.0
(i)
Aerosol II                       mg/ml
Compound X                       0.2
Sorbitan trioleate               0.27
Trichlorofluoromethane           70.0
Dichlorodifluoromethane          280.0
Dichlorotetrafluoroethane        1094.0
(j)
Aerosol III                      mg/ml
Compound X                       2.5
Sorbitan trioleate               3.38
Trichlorofluoromethane           67.5
Dichlorodifluoromethane          1086.0
Dichlorotetrafluoroethane        191.6
(k)
Aerosol IV                       mg/ml
Compound X                       2.5
Soya lecithin                    2.7
Trichlorofluoromethane           67.5
Dichlorodifluoromethane          1086.0
Dichlorotetrafluoroethane        191.6
(l)
Ointment                         ml
Compound X                       40 mg
Ethanol                          300 μl
Water                            300 μl
1-Dodecylazacycloheptan-2-one    50 μl
Propylene glycol                 to 1 ml
Note:
Compound X in the above formulation may comprise a compound illustrated in Examples. The above formulations may be obtained by conventional procedures well known in the pharmaceutical art. The tablets (a)-(c) may be enteric coated by conventional means, for
# example to provide a coating of cellulose acetate phthalate. The aerosol formulations (h)-(k) may be used in conjunction with standard, metered dose aerosol dispensers, and the suspending agents sorbitan trioleate and soya lecithin may be replaced by an alternative
# suspending agent such as sorbitan monooleate, sorbitan sesquioleate, polysorbate 80, polyglycerol oleate or oleic acid.

Claims

1. A compound of formula (I)

X is CH2 or SO2

R1 is an optionally substituted aryl or heteroaryl ring;

R2 is carboxy, cyano, —C(O)CH2OH, —CONHR8, —SO2NHR9, tetrazol-5-yl, SO3H, or a group of formula (VI)

where R8 is selected from hydrogen, alkyl, aryl, cyano, hydroxy, —SO2R12 where R12 is alkyl, aryl, heteroaryl, or haloalkyl, or R8 is a group —(CHR13)r—COOH where r is an integer of 1-3 and each R13 group is independently selected from hydrogen or alkyl; R9 is hydrogen, alkyl, optionally substituted aryl such as optionally substituted phenyl or optionally subtituted heteroaryl such as 5 or 6 membered heteroaryl groups, or a group COR14 where R14 is alkyl, aryl, heteroaryl or haloalkyl; R10 and R11 are independently selected from hydrogen or alkyl, particularly C1-4alkyl;

R3 is hydrogen a functional group, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted alkoxy, optionally substituted aralkyl, optionally substituted aralkyloxy, optionally substituted cycloalkyl;

R4 is a group NHCOR15, NHSO2R15 or OCONR16R17 where R15 is optionally substituted alkyl, optionally substituted aryl or optionally substituted heteroaryl and R16 and R17 are independently selected from hydrogen, optionally substituted alkyl, optionally substituted aryl and optionally substituted heteroaryl, with the proviso that at least one of R16 or R17 is other than hydrogen, or R16 and R17 together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring which optionally contains further heteroatoms; and

R5, R6 and R7 are independently selected from hydrogen, a functional group or an optionally substituted hydrocarbyl groups or optionally substituted heterocyclic groups; and further provided that when R4 is a group NHCOR15, R15 is substituted alkyl, optionally substituted aryl or optionally substituted heteroaryl.

2. A compound according to claim 1 wherein a group R15, R16 and R17 as they appear in the definition of R4, is substituted by at least one functional group, or an aryl or heterocyclyl groups, either of which may themselves be substituted by one or more functional groups or further aryl or heterocyclyl groups.

3. A compound according to any one of the preceding claims wherein R4 is a group NHCOR15 or NHSO2R15 and R15 is a substituted alkyl group or an optionally subsituted hetercyclyl or optionally substituted phenyl group.

4. A compound according to claim 3 wherein R15 is alkyl substituted by a group of formula NR19R20 where R19 and R20 are independently selected from hydrogen or optionally substituted hydrocarbyl, or R19 and R20 together form an optionally substituted ring which optionally contains further heteroatoms such as S(O)m, oxygen and nitrogen, n is an integer of 1 or 2, m is 1 or 2.

5. A compound according to any one of the preceding claims where R2 is carboxy.

6. A compound according to any one of the preceding claims wherein R1 is 3,4-dichlorophenyl, 3-fluoro-4-chlorophenyl, 3-chloro-4-fluorophenyl or 2,3-dichloropyrid-5-yl.

7. A compound according to any one of the preceding claims where X is CH2.

8. A process for preparing a compound according to claim 1 which process comprises either

(a) where R4 is NHCOR15or NHSO2R15, reacting a compound of formula (VII)

where X, R1, R3, R5, R6 and R7 are as defined in claim 1, and R2 is a group R2 as defined in relation to formula (I) or a protected form thereof, with a compound of formula (VIII)

Z-R22  (VIII)

where Z is a leaving group and R22 is a group COR15′ or SO2R15′ where R15′ is group R15 as defined in relation to formula (I) or a precursor thereof;

or (b) where R4 is a group OCONR16R17, reacting a compound of formula (VIIA)

where X, R2′, R1, R3, R5, R6 and R7 are as defined claim 1 and R2 is a group R2 as defined in claim 1 or a protected form thereof, with a compound of formula (VIIIA)

Z-CONR16R17  (VIIIA)

where Z, R16 and R17 are as defined above;

and thereafter if desired or necessary:

(i) converting a precursor group R15′ to a group R15 and/or converting a group R15 to a different such group;

(ii) deprotecting a group R2′ to a group R2.

9. A pharmaceutical composition comprising a compound according to any one of claims 1 to 7 in combination with a pharmaceutically acceptable carrier.

10. A compound according to any one of claims 1 to 7 for use in the preparation of a medicament for use in the treatment of disease mediated by monocyte chemoattractant protein-1 or RANTES Regulated upon Activation, Normal T-cell Expressed and Secreted), such as inflammatory disease.