I. BACKGROUND OF THE INVENTION The present invention pertains to a method for isolating a polynucleotide of interest that is present in the genome of a mycobacterium strain and/or is expressed by said mycobacterium strain and that is absent or altered in the genome of a different mycobacterium strain and/or is not expressed in said different mycobacterium strain, said method comprising the use of at least one clone belonging to a genomic DNA library of a given mycobaterium strain, said DNA library being cloned in a bacterial artificial chromosome (BAC). The invention concerns also polynucleotides identified by the above method, as well as detection methods for mycobacteria, particularly Mycobacterium tuberculosis, and kits using said polynucleotides as primers or probes. Finally, the invention deals with BAC-based mycobacterium DNA libraries used in the method according to the invention and particularly BAC-based Mycobacterium tuberculosis and Mycobacterium bovis BCG DNA libraries.
Radical measures are required to prevent the grim predictions of the World Health Organisation for the evolution of the global tuberculosis epidemic in the next century becoming a tragic reality. The powerful combination of genomics and bioinformatics is providing a wealth of information about the etiologic agent, Mycobacterium tuberculosis, that will facilitate the conception and development of new therapies. The start point for genome sequencing was the integrated map of the 4.4 Mb circular chromosome of the widely-used, virulent reference strain, M. tuberculosis H37Rv and appropriate cosmids were subjected to systematic shotgun sequence analysis at the Sanger Centre.
Cosmid clones (Balasubramanian et al., 1996; Pavelka et al., 1996) have played a crucial role in the M. tuberculosis H37Rv genome sequencing project. However, problems such as under-representation of certain regions of the chromosome, unstable inserts and the relatively small insert size complicated the production of a comprehensive set of canonical cosmids representing the entire genome.
II. SUMMARY OF THE INVENTION In order to avoid the numerous technical constraints encountered in the state of the art, as decribed hereabove, when using genomic mycobacterial DNA libraries constructed in cosmid clones, the inventors have attempted to realize genomic mycobacterial DNA libraries in an alternative type of vectors, namely Bacterial Artificial Chromosome (BAC) vectors.
The success of this approach depended on whether the resulting BAC clones could maintain large mycobacterial DNA inserts. There are various reports describing the successful construction of a BAC library for eucaryotic organisms (Cai et al., 1995; Kim et al., 1996; Misumi et al., 1997; Woo et al., 1994; Zimmer et al., 1997) where inserts up to 725 kb (Zimmer et al., 1997) were cloned and stably maintained in the E. coli host strain.
Here, it is shown that, surprisingly, the BAC system can also be used for mycobacterial DNA, as 70% of the clones contained inserts in the size of 25 to 104 kb.
This is the first time that bacterial, and specifically mycobacterial, DNA is cloned in such BAC vectors.
In an attempt to obtain complete coverage of the genome with a minimal overlapping set of clones, a Bacterial Artificial Chromosome (BAC) library of M. tuberculosis was constructed, using the vector pBeloBAC11 (Kim et al., 1996) which combines a simple phenotypic screen for recombinant clones with the stable propagation of large inserts (Shizuya et al., 1992). The BAC cloning system is based on the E. coli F-factor, whose replication is strictly controlled and thus ensures stable maintenance of large constructs (Willets et al., 1987). BACs have been widely used for cloning of DNA from various eucaryotic species (Cat et al., 1995; Kim et al., 1996; Misumi et al., 1997; Woo et al., 1994; Zimmer et al., 1997). In contrast, to our knowledge this report describes the first attempt to use the BAC system for cloning bacterial DNA.
A central advantage of the BAC cloning system over cosmid vectors used in prior art is that the F-plasmid is present in only one or a maximum of two copies per cell, reducing the potential for recombination between DNA fragments and, more importantly, avoiding the lethal overexpression of cloned bacterial genes. However, the presence of the BAC as just a single copy means that plasmid DNA has to be extracted from a large volume of culture to obtain sufficient DNA for sequencing and it is described here in the examples a simplified protocol to achieve this.
Further, the stability and fidelity of maintenance of the clones in the BAC library represent ideal characteristics for the identification of genomic differences possibly responsible for phenotypic variations in different mycobacterial species.
As it will be shown herein, BACs can be allied with conventional hybridization techniques for refined analyses of genomes and transcriptional activity from different mycobacterial species.
Having established a reliable procedure to screen for genomic polymorphisms, it is now possible to conduct these comparisons on a more systematic basis than in prior art using representative BACs throughout the chromosome and genomic DNA from a variety of mycobacterial species.
As another approach to display genomic polymorphisms, the inventors have also started to use selected H37Rv BACs for “molecular combing” experiments in combination with fluorescent in situ hybridization (Bensimon et al., 1994; Michalet et al., 1997). With such technique the one skilled in the art is enabled to explore the genome of mycobacteria in general and of M. tuberculosis in particular for further polymorphic regions.
The availability of BAC-based genomic mycobacterial DNA libraries constructed by the inventors have allowed them to design methods and means both useful to identify genomic regions of interest of pathogenic mycobacteria, such as Mycobacterium tuberculosis, that have no counterpart in the corresponding non-pathogenic strains, such as Mycobacterium bovis BCG, and useful to detect the presence of polynucleotides belonging to a specific mycobacterium strain in a biological sample.
By a biological sample according to the present invention, it is notably intended a biological fluid, such as plasma, blood, urine or saliva, or a tissue, such as a biopsy.
Thus, a first object of the invention consists of a method for isolating a polynucleotide of interest that is present in the genome of a mycobacterium strain and/or is expressed by said mycobacterium strain and that is absent or altered in the genome of a different mycobacterium strain and/or is not expressed in said different mycobacterium strain, said method comprising the use of at least one clone belonging to a genomic DNA library of a given mycobaterium strain, said DNA library being cloned in a bacterial artificial chromosome (BAC).
The invention is also directed to a polynucleotide of interest that has been isolated according to the above method and in partoular a polynucleotide containing one or several Open Reading Frames (ORFs), for example ORFs encoding either a polypeptide involved in the pathogenicity of a mycobacterium strain or ORFs encoding Polymorphic Glycine Rich Sequences (PGRS).
Such polynucleotides of interest may serve as probes or primers in order to detect the presence of a specific myobacterium strain in a biological sample or to detect the expression of specific genes in a particular mycobacterial strain of interest.
The BAC-based genomic mycobacterial DNA libraries generated by the present inventors are also part of the invention, as well as each of the recombinant BAC clones and the DNA insert contained in each of said recombinant BAC clones.
The invention also pertains to methods and kits for detecting a specific mycobacterium in a biological sample using either at least one recombinant BAC clone or at least one polynucleotide according to the invention, as well as to methods and kits to detect the expression of one or several specific genes of a given mycobacterial strain present in a biological sample.
III. BRIEF DESCRIPTION OF THE FIGURES In order to better understand the present invention, reference will be made to the appended figures which depicted specific embodiments to which the present invention is in no case limited in scope with.
FIG. 1: PCR-screening for unique BAC clones with specific primers for 2 selected genomic regions of the H37Rv chromosome, using 21 pools representating 2016 BACs (Panel A) and sets of 20 subpools from selected positive pools (Panel B).
FIG. 2: Pulsed-field gel electrophoresis gel of DraI-cleaved BAC clones used for estimating the insert sizes of BACs.
FIG. 3: Minimal overlapping BAC map of M. tuberculosis H37Rv superimposed on the integrated physical and genetic map established by Philipp et al. (18). Y- and I-numbers show pYUB328 (2) and pYUB412 (16) cosmids which were shotgun sequenced during the H37Rv genome sequencing project. Y-cosmids marked with * were shown in the integrated physical and genetic map (18). Rv numbers show the position of representative BAC clones relative to sequenced Y- and I-clones. Squared Rv numbers show BACs which were shotgun sequenced at the Sanger Centre.
FIG. 4: Ethidium bromide stained gel (Panel A) and corresponding Southern blot (Panel B) of EcoRI and PvuII digested Rv58 DNA hybridized with 32P labeled genomic DNA preparations from M. tuberculosis H37Rv, M. bovis ATCC 19210 and M. bovis BCG Pasteur.
FIG. 5: Organisation of the ORFs in the 12.7 kb genomic region present in M. tuberculosis H37Rv but not present in M. bovis ATCC 19210 and M. bovis BCG Pasteur. Arrows show the direction of transcription of the putative genes. Positions of EcoRI and PvuII restriction sites are shown. Vertical dashes represent stop codons. The 11 ORFs correspond to the ORFs MTCY277.28 to MTCY277.38/accession number Z79701-EMBL Nucleotide Sequence Data Library. The junction sequences flanking the polymorphic region are shown.
FIG. 6: Variation in the C-terminal part of a PE-PGRS open reading frame in M. tuberculosis strain H37Rv relative to M. bovis BCG strain Pasteur.
The numbers on the right side of the Figure denote the position of the end nucleotides, taking as the reference the M. tuberculosis genome.
FIG. 7: Polynucleotide sequence next to the HindIII cloning site in the BAC vector pBeloBAC11 (Kim et al., 1996) used to clone the inserts of the BAC-based myobacterial genomic DNA library according to the invention.
- NotI: location of the NotI restriction sites.
- Primer T7-BAC1: nucleotide region recognized by the T7-BAC 1 primer shown in Table 1.
- T7 promoter: location of the T7 promoter region on the pBeloBac11 vector.
- Primer T7-Belo2: nucleotide region recognized by the T7-Belo2 pimer shown in Table 1.
- Hind III: the HindIII cloning site used to clone the genomic inserts in the pBeloBAC11 vector.
- SP6-Mid primer: nucleotide region recognized by the SP6 Mid primer shown in Table 1.
- SP6-BAC1 primer: nucleotide region recognized by the SP6 BAC1 primer shown in Table 1.
- SP6 promoter location of the SP6 promoter region on the pBeloBac11 vector.
IV. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS As already mentioned hereinbefore, the present invention is directed to a method for isolating a polynucleotide of interest that is present in the genome of a mycobacterium strain and/or is expressed by said mycobacterium strain and that is absent or altered in the genome of a different mycobacterium strain and/or is not expressed in said different mycobacterium strain, said method comprising the use of at least one clone belonging to a genomic DNA library of a given mycobaterium strain, said DNA library being cloned in a bacterial artificial chromosome (BAC).
For this purpose, the inventors have constructed several BAC-based mycobacterial genomic DNA libraries that may be used in order to perform the above described method.
Because it is the first time that mycobacterial genomic DNA has been successfully cloned in BAC type vectors, and because these DNA libraries are then novel and nonobvious, an object of the present invention consists in a myobacterial genomic DNA library cloned in such a BAC type vector.
As an illustrative example, a BAC-based DNA library of Mycobacterium tuberculosis has been realized. Forty-seven cosmids chosen from the integrated map of the 4.4 Mb circular chromosome (Philipp et al., 1996a) were shotgun-sequenced during the initial phase of the H37Rv genome sequence project. The sequences of these clones were used as landmarks in the construction of a minimally overlapping BAC map. Comparison of the sequence data from the termini of 420 BAC clones allowed us to establish a minimal overlapping BAC map and to fill in the existing gaps between the sequence of cosmids. As well as using the BAC library for genomic mapping and sequencing, we also tested the system in comparative genomic experiments in order to uncover differences between two closely related mycobacterial species. As shown in a previous study (Philipp et al., 1996b), M. tuberculosis, M. bovis and M. bovis BCG, specifically BCG Pasteur strain, exhibit a high level of global genomic conservation, but certain polymorphic regions were also detected. Therefore, it was of great interest to find a reliable, easy and rapid way to exactly localize polymorphic regions in mycobacterial genomes using selected BAC clones. This approach was validated by determining the exact size and location of the polymorphisms in the genomic region of DraI fragment Z4 (Philipp et al., 1996b), taking advantage of the availability of an appropriate BAC clone covering the polymorphic region and the H37Rv genome sequence data. This region is located approximately 1.7 Mb from the origin of replication.
The Bacterial Artificial Chromosome (BAC) cloning system is capable of stably propagating large, complex DNA inserts in Escherichia coli. As part of the Mycobacterium tuberculosis H37Rv genome sequencing project, a BAC library was constructed in the pBeloBAC11 vector and used for genome mapping, confirmation of sequence assembly, and sequencing. The library contains about 5000 BAC clones, with inserts ranging in size from 25 to 104 kb, representing theoretically a 70 fold coverage of the M. tuberculosis genome (4.4 Mb). A total of 840 sequences from the T7 and SP6 termini of 420 BACs were determined and compared to those of a partial genomic database. These sequences showed excellent correlation between the estimated sizes and positions of the BAC clones and the sizes and positions of previously sequenced cosmids and the resulting contigs. Many BAC clones represent linking clones between sequenced cosmids, allowing fall-coverage of the H37Rv chromosome, and they are now being shotgun-sequenced in the framework of the H37Rv sequencing project. Also, no chimeric, deleted or rearranged BAC clones were detected, which was of major importance for the correct mapping and assembly of the H37Rv sequence. The minimal overlapping set contains 68 unique BAC clones and spans the whole H37Rv chromosome with the exception of a single gap of ˜150 kb. As a post-genomic application, the canonical BAC set was used in a comparative study to reveal chromosomal polymorphisms between M. tuberculosis, M. bovis and M. bovis BCG Pasteur, and a novel 12.7 kb segment present in M. tuberculosis but absent from M. bovis and M. bovis BCG was characterized. This region contains a set of genes whose products show low similarity to proteins involved in polysaccharide biosynthesis. The H37Rv BAC library therefore provides the one skilled in the art with a powerful tool both for the generation and confirmation of sequence data as well as for comparative genomics and a plurality of post-genomic applications.
The above described BAC-based Mycobacterium tuberculosis genomic DNA library is part of the present invention and has been deposited in the Collection Nationale de Cultures de Microorganismes (CNCM) on Nov. 19, 1997 under the accession number I-1945.
Another BAC-based DNA library has been constructed with the genomic DNA of Mycobacterium bovis BCG, Pasteur strain, and said DNA library has been deposited in the Collection Nationale de Cultures de Microorganismes (CNCM) on XX XX, 1998 under the accession number I-XXXX.
Thus, as a specific embodiment of the above described method for isolating a polynucleotide of interest said method makes use of at least one BAC-based DNA library that has beeen constructed from the genomic DNA of Mycobacterium tuberculosis, more specifically of the H37Rv strain and particularly of the DNA library deposited in the accession number I-1945.
In another specific embodiment of the above described method for isolating a polynucleotide of interest said method makes use of at least one BAC-based DNA library has beeen constructed from the genomic DNA of Mycobacterium bovis BCG, more specifically of the Pasteur strain and particularly of the DNA library deposited in the accession number I-XXXX.
In more details, the method according to the invention for isolating a polynucleotide of interest may comprise the following steps:
-
- a) Isolating at least one polynucleotide contained in a clone of a BAC-based DNA library of mycobacterial origin;
- b) Isolating:
- at least one genomic or cDNA polynucleotide from a mycobacterium, said mycobacterium belonging to a strain different from the strain used to construct the BAC-based DNA library of step a); or alternatively
- at least one polynucleotide contained in a clone of a BAC-based DNA library prepared from the genome of a mycobacterium that is different from the mycobacterium used to construct the BAC-based DNA library of step a);
- c) Hybridizing the at least one polynucleotide of step a) to the at least one polynucleotide of step b);
- d) Selecting the at least one polynucleotide of step a) that has not formed a hybrid complex with the at least one polynucleotide of step b);
- e) Characterizing the selected polynucleotide.
Following the above procedure, the at least one polynucleotide of step a) may be prepared as follows:
-
- 1) Digesting at least one recombinant BAC clone by an appropriate resctriction endonuclease in order to isolate the polynucleotide insert of interest from the vector genetic material;
- 2) Optionally amplifying the resulting polynucleotide insert;
- 3) Optionally digesting the polynucleotide insert of step 1) or step 2) with at least one restriction endonuclease.
The above method of the invention allows the one skilled in the art to perform comparative genomics between different strains or species of mycobacteria cells, for example between pathogenic strains or species and their non pathogenic strains or species counterparts, as it is the illustrative case for the genomic comparison between Mycobacterium tuberculosis and Mycobacterium bovis BCG that is described herein in the examples.
Restriction digests of a given clone of a BAC library according to the invention may be blotted to membranes, and then probed with radiolabeled DNA form another strain or another species of mycobacteria, allowing the one skilled in the art to identify, characterize and isolate a polynucleotide of interest that may be involved in important metabolical and/or physiological pathways of the mycobacterium under testing, such as a polynucleotide functionally involved in the pathogenicity of said given mycobacteria for its host organism.
More specifically, the inventors have shown in Example 6 that when restriction digests of a given clone of the BAC library identified by the CNCM accession number I-1945 are blotted to membranes and then probed with radiolabeled total genomic DNA from, for example, Mycobacterium bovis BCG Pasteur, it is observed that restriction fragments that fail to hybridize with the M. bovis BCG Pasteur DNA are absent from its genome, hence identifying polymorphic regions between M. bovis BCG Pasteur and M. tuberculosis H37Rv.
Thus, a further object of the present invention consists in a polynucleotide of interest that has been isolated according to the method described herein before.
In Example 6, a polynucleotide of approximately 12.7 kilobases has been isolated that is present in the genome of M. tuberculosis but is absent of the genome of M, bovis BCG. This polynucleotide of interest contains 11 ORFs that may be involved in polysaccharide biosynthesis. In particular, two of said ORFs are of particular interest, namely ORF6 (MTCY277.33; Rv1511) that encodes a protein that shares significant homology with bacterial GDP-D-mannose dehydratases, whereas the protein encoded by ORF7 (MTCY277.34; Rv1512) shares significant homology with a nucleotide sugar epimerase. As polysaccharide is a major constituent of the mycobacterial cell wall these deleted genes may cause the cell wall of M. bovis BCG to differ from that of M. tuberculosis, a fact that may have important consequences for both the immune response to M. bovis BCG and virulence. Detection of such a polysaccharide is of diagnostic interest and possibly useful in the design of tuberculosis vaccines.
Consequently, the polynucleotide of interest obtained following the method according to the invention may contain at least one ORF, said ORF preferably encoding all or part of a polypeptide involved in an important metabolical and/or physiological pathway of the mycobacteria under testing and more specifically all or part of a polypeptide that is involved in the pathogenicity of the mycobacteria under testing, such as for example Mycobacterium tuberculosis, and more generally mycobacteria belonging to the Mycobacterium tuberculosis complex.
The Mycobacterium tuberculosis complex has its usual meaning, i.e. the complex of mycobacteria causing tuberculosis which are Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium africanum, Mycobacterium microti and the vaccine strain Mycobacterium bovis BCG.
An illustrative polynucleotide of interest according to the present invention comprises all or part of the polynucleotide of approximately 12.7 kilobases that is present in the genome of M. tuberculosis but is absent from the genome of M. bovis BCG disclosed hereinbefore. This polynucleotide is contained in clone Rv58 of the BAC DNA library I-1945.
Generally, the invention also pertains to a purified polynucleotide comprising the DNA insert contained in a recombinant BAC vector belonging to a BAC-based mycobacterial genomic DNA library, such as for example the I-1945 BAC DNA library.
Advantageously, such a polynucleotide has been identified according to the method of the invention.
Such a polynucleotide of interest may be used as a probe or a primer useful for specifically detecting a given mycobacterium of interest, such as Mycobacterium tuberculosis or Mycobacterium bovis BCG.
More specifically, the invention then deals with a purified polynucleotide useful as probe or a primer comprising all or part of the nucleotide sequence SEQ ID No1:
ACCTGCGCTT GCAGAGATCA AATAGGGCGC ATGGGTCAGC 60
ATAGTACAGG TCGTCGCGCA
TCTTTGATGC ATCGGAATAA GATGTCAGGC AATTAAAAGA 120
GAAGCCACGG CGACTCGCGG
CATTCAGCAT GTCGAGCGTC GCTTCGATGT GAGCGCACCA 180
TTCCGTGTCC AACGATTTCA
GACGAACATT GAATATTCCA CTCGCGACGC TATAGTCCGC 240
CTCCCGATCT ATGCGCGCCG
CGCAGATGAA GTCTGCGTTC GCCCGACCTT CGAAACGTAG 300
TGCGGCCGCG CGCACCATTT
CGGGGGAGAC GTCGATGCCG GTGTAATCAG TTTTGAAGCC 360
ACGCGCATCT AGGTAGTCCA
GTAGAGCCCC ATAGCCACAG CCTAGATCGT TGATCGAAAA 420
TGGGTCCGCC GCATTGACAA
TGCGCACCAG CTGGTCAAAG CGCAACGCCT GCCCGGCTTC 480
GCCGTTCCAA TCGACGCCGC
GCGGGTGCCG TGTGCTTCGA GTTTCGATGC GTAGTAACGG 540
GCCACGTCAG CGAGCATGGT
CGTTGCGTCT TCCGCCATGA AGCTGCCTCA CGATTTGTGT 600
GTGTGGGCGT CGGTGCGTGG
GTCCGAGACT ATACCTTCAA CAGTTGCATG CCGAGGCTGC 660
GGCGGGCAAT GACCCAAAAA
CCCGCCGGCA CGGTTCGCCG AGCAAGGAAG CGTGGAGACG 720
ATAGATAATT TCACTGGCGA
CAGTACCTCA AATAGTCCGG AGCCTCGGCT CCGACGTTAA 780
AGAGCAGATC CAGAATCGAC
ACGGCGGGCT CGAACCCTCC CCACAATTGC TTATAATCGC 840
GGTAGCCGTC ATAATCGAAC
CAAGTTACCC GGATGCTAAG TTCGTCGAAC ACGCGCTCAT 900
CGACATACGA ACGGGCTGAG
GGGCCAGAGA CATATTCGGT CGCTGCGGCC TGTTGGCAGA 960
GGTTGGCCAG TCTCTCGGTC
TTGCCGTCGG CTAATTCGTA GTCCCACGAA TTTGCCAGTC 1020
GCGTGCTGAT ACCGAGATAA
CTGCAAATCG CATTCAATAG ACGCCTGTTG AGTAAGGAAA 1080
GATTCGTGTG CTGTTCTTCG
AGGTAAATCG GCGCGAGCCA GTCAGCGATC TCCGCAAAAT 1140
GAGCGGCCGC GCTGTAGTTG
AATTCTAGTG CCCGCCAGTG CGCTTTCGCC CAATCGGTGC 1200
CGTCGATCAG CGTCTCACGT
ATCTTTTGAT GGAAACGTCC CTTCACCTGG ACGGGAACAG 1260
TTATCCACTG TAACCCCTGG
CTCGTTTTGA TCCGATTTCT GTTTCGCCAA TCACGCTTGG 1320
TATATTGCAT GTCATCATAG
ATGATGAATT CATCGACGAA TGCAATCAGG TCAAAATATC 1380
CTCGCCAAGG TATGTAATTT
GATTGAACAA TCGCGACTTT CTTCAACGCG GTGTCTCCAA 1440
TTTAGAATAA CAAATACGTC
GCGCCCGCGA CAGCTCCGCT GGAGCGAGTT CAAGCGATTC 1500
TGCGACATAT TCAATATGGT
GCTCGGGAAG GCCAGGATGG GCCGCGACCC GGGGCGTCCG 1560
GTGCGCGATG AACGTCGCAT
CGTCTCCTGT GAGATAATTG CATCCGATCA TATAGGGCTG 1620
GCTGCGGCTA GGTTGCTGGC
AAAAAGATAT CGCGGCCGAT CCGTTTCTGG TTTTGTCTTG 1680
ATGATCAAAT CCGCTTCCGT
TCACGAGATC GATTCCTGGT CTTCCCCCAG CGTCGCGATG 1740
TCGATAGGTG TCGCGCTTTG
TTCGTACCCG CACTACGCGG CGGCGAGAAC CTCGCCACCG 1800
AATCGGGATT GGGGGGAGGA
TACCACTCGG TCGAGGCCCG TCACCGGCCT TCTAGCGGGT 1860
TGACCATCAG TGTTTGCAGG
GCCCTATCCC GGTATGGCGC ACCACGGGAT CGGCAGCGTT 1920
CCGGTTGCTG GCGTGGTACC
TCGTTGTGGC GCCGTGGTCC ATGTCGATTG AGTGCGTGGA 1980
TCAGTGTAAA CCGTTGCGCG
CCATGTTCTG TAGGCACTGG TTCGGGTTGT GGTTAGGCTG 2040
CACGGTTGGC AGGTTACCAA
CCACTGAGCC CCTGGGCGGA TGTGAGCTCG GACTCCGCCT 2100
ATGGGGTGTA ATTTTGGCAG
ATTGGGCCGG GTCCCCGTGG TGAGGACTCC TCAACCGGAT 2160
TGGGTAAGCA TGAGGTGGTG
CTGGCAGCGG TGTCCTGGTC GCTCTCCCGA GTAGGCCCGT 2220
TGTGACTGTC ATGTGGGCGA
GCGGGTTTGC GCGCGTAGGA GACGATGATT ACTACGCACG 2280
TGACCAACCA CAAGAACGGT
GCCCATGTCA CCGTGGTGAA AACGAGTGGC GTGGTACCGA 2340
CTACCCCTTT GGCTCCCAGC
TGTCCATAGA GCGGCACGTA GAACGGCTGG CCCGGGACCG 2400
CGACGTTGAC GATGCTCAGC
GCCACGGCCA AACTCACGCA GACGCCGACC GCGCGGCGGC 2460
GGTCTCCATG GGCTGCGAGT
TGGTCGAATA TCCCAGCACC AGGAGGCCCG TTGGGCTCTC 2520
GGGCTACCAG TGCAGCGATT
GGCAAGACGA AAACGAGATA GTAGAAGGCG ACGTCCGCGG 2580
GGGAGAAGGT GGCGGTGGCG
AGCAACACAA TCCCCACCAT GACAGGCGGG ATACGGCGTC 2640
CGAGCGCCAG CACGGCGACC
ACGACTATGA CTAGGACAGC AAACCCGATC TGCGTTCGCG 2700
GACCAGTGAG GAAACCCTCT
GGGATCTTGC CCGATTGATA GTTCTTGATG CTATCGGGGA 2760
TCAGCAGGAG TGCCTTGCCA
AAGGACACGT TCCGCGGGTC TCGAAGCCCT CCGAACGAAC 2820
TATTGAACTT GATGATGCCG
TGGATCGACT GTGCGATCGT CCCCGGGAAG CCTCGTGGCC 2880
ACAACAGAAA GGCTGCGATA
TTGGACACCA CCACGCCGGT GATCCCGATA CCAGCCCACC 2940
GCCATTGTCG AGCCGCCAAC
AACACCACGC CGAGAACGAC GAACTGCGGC TTTACCAGGA 3000
CGGCCAAGAT CACCGTGATG
GTGGCGAGGC CCCACCGCTG TCGGGACAAC GCCACGAAGT 3060
AAGCCAGCGC GATCGGTACC
ACGAACCCTG TCGAGTTGCC TCGATCGATG ACCCCCCACG 3120
CCGGGATGGC CGCGGCGCCC
AGTGTCACGA AGATGACCAC TCGCTCCAGA CCACGTGCCC 3180
CCCGGGCCGC CCAGATGGCG
GGAGATATGA CCGCCATCGT TAGGGCGACC AGGTAACAGA 3240
TCAGCCCCAA GCGCGGCGCA
CCCAGCCAAT GGCTGGGTAG TCCGAAAATC GCATACGGTA 3300
TGCGGGCGGG GGCCCATGCA
GCAACCGCGG TCGGCTGGTA ATCGGCGGGT AGCGAGATCA 3360
GGTAGTCCGC GGGATTGGGT
TGAATCCCGG CGGCGGCGAC CATGGCGTAG TCGCTGAAGC 3420
AGTGCCGACC GATATTCATG
CCCCAATCAA GCCAACAGTC CCCAGGGACT ACCAAAAGAG 3480
TGGAAAAGAC GTCGACCGCG
TACCACTGAC TGAGGGCGTA CGCCGTCGCC GCCGAAATCA 3540
CCGACGCCAG CAGGATGGTG
CCGAGCATGA GGGTGCGCTC GGATTGGGAG CCGATCGCCC 3600
AGAGCCGCTC CCGGCTCGCG
GTCACGGCAC CGCGCAACAC CTCCGGGGGT CGCTTCATCT 3660
GGATTCTCCT CGGTTCTGCG
CGAAACGGTA GCAGAGCGCC ATGGTTGCCA ACGCGGTCGC 3720
CGGGCAGTCT AGACCGGATC
TTCCTCCTGG CAACCGACAA CAGGACGTCG TTGCCGAAAG 3780
GGCGCTGGGC ACCGACATCT
AGGATGAACC CACAGCCACG CCCCGACGTT ATGCCATGGC 3840
GAAGAGCGAC CGGCAGGAGC
GGGAACCCAG TGAAGCGAGC GCTCATCACC GGAATCACAG 3900
GACCGGACGG CTCGTATCTC
GCTAAGCTCC CGCTGAAGGG ATATGTGCCC GCTGGTAGCC 3960
CGGCCGAGGT CTATTTCTGC
TGGGCGACAC GGAATTATCG CGAATTGTAT GGCTTGCTCG 4020
CGGTCAACAG CATCTGGTTC
AATCACGAAT CACCGCGTCA CGGCGAGACA TTCATGACTC 4080
GTAATCCTGC ACCATATCGC
GGTCGGCAAC GAGGCGCTGA TCGATGCGCA GACGCTGATG 4140
CGCCCGCCCA CCCGGATAGG
TATCAGTATT GGGGCGTTCC GGCCAGCGTA CGAGGCGTGA 4200
TCGACCGCGC AATGGGTGTT
TGCGTTGAGT AATAATCTGA ACCGTGTGAA CGCATGCATG 4260
GATGCATTCC TTGCCCGTAT
CCGCTCACAT GTTGATGCGC ACGCGCCAGA ATTGCGTTCA 4320
CTGTTCGATA CGATGGCGGC
CGAGGCCCGA TTTGCACGCG ACTGGCTGTC CGAGGACCTC 4380
GCGCGGTTGC CTGTCGGTGC
AGCATTGCTG GAAGTGGGCG GGGGGGTACT TCTGCTCAGC 4440
TGTCAACTGG CGGCGGAGGG
ATTTGACATC ACCGCCATCG AGCCGACGGG TGAAGGTTTT 4500
GGCAAGTTCA GACAGCTTGG
CGACATCGTG CTGGAATTGG CTGCAGCACG ACCCACCATC 4560
GCGCCATGCA AGGCGGAAGA
CTTTATTTCC GAGAAGCGGT TCGACTTCGC CTTCTCGCTG 4620
AATGTGATGG AGCACATCGA
CCTTCCGGAT GAGGCAGTCA GGCGGGTATC GGAAGTGCTG 4680
AAACCCGGGG CCAGTTACCA
CTTCCTGTGC CCGAATTACG TATTCCCGTA CGAACCGCAT 4740
TTCAATATCC CAACATTCTT
CACCAAAGAG CTGACATGCC GGGTGATGCG ACATCGCATC 4800
GAGGGCAATA CGGGCATGGA
TGACCCGAAG GGAGTCTGGC GTTCGCTCAA CTGGATTACG 4860
GTTCCCAAGG TGAAACGCTT
TGCGGCGAAG GATGCGACGC TGACCTTGCG CTTCCACCGT 4920
GCAATGTTGG TATGGATGCT
GGAACGCGCG CTGACGGATA AGGAATTCGC TGGTCGCCGG 4980
GCACAATGGA TGGTCGCTGC
TATTCGCTCG GCGGTGAAAT TGCGTGTGCA TCATCTGGCA 5040
GGCTATGTTC CCGCTACGCT
GCAGCCCATC ATGGATGTGC GGCTAACGAA GAGGTAATGA 5100
CATGGCGCAA GCGACATCGG
GCATTCGCGC GGCACTTTCG CAACCTGCTG TGTATGAGGC 5160
GTATCAGCGG ATTGCGGGCG
CTAAAAGCGG GCTTGCGTGG ATCACAACCG ACCCCATCCA 5220
GTCGTTGCCA GGCATGCGTA
CTCTCGACCT CGGTTGCTGG CCAGCGGTGA TACACAGCTC 5280
CCCGCCAGTG GACGTGACAT
GTACGAGAGA CGGCATGAGC GCGGAATGTG CGACCGTGCC 5340
GTCGAGATGA CCGACGTCGG
CGCTACGGCA GCCCCCACCG GACCTATCGC GCGGGGCAGC 5400
GTCGCTCGGG TCGGCGCGGC
GACCGCGTTG GCCGTTGCCT GCGTCTACAC GGTCATCTAT 5460
CTGGCGGCCC GCGACCTACC
CCCGGCTTGT TTTTCGATAT TCGCGGTGTT TTGGGGGGCG 5520
CTCGGCATTG CCACCGGCGC
CACCCACGGC CTCCTGCAAG AAACGACCCG CGAGGTCCGC 5580
TGGGTGCGCT CCACCCAAAT
AGTTGCGGGC CATCGTACCC ATCCGCTGCG GGTGGCCGGG 5640
ATGATTGGCA CCGTCGCGGC
CGTCGTAATT GCGGGTAGCT CACCGCTGTG GAGCCGACAG 5700
CTATTCGTCG AGGGGCGCTG
GCTGTCCGTG GGGCTACTCA GCGTTGGGGT GGCCGGGTTC 5760
TGCGCGCAGG CGACCCTGCT
GGGCGCGCTG GCCGGCGTCG ACCGGTGGAC ACAGTACGGG 5820
TCACTGATGG TGACCGACGC
GGTCATCCGG TTGGCGGTCG CCGCGGCAGC GGTTGTGATC 5880
GGATGGGGTC TGGCCGGGTA
CTTGTGGGCC GCCACCGCGG GAGCGGTGGC GTGGCTGCTC 5940
ATGCTGATGG CCTCGCCCAC
CGCGCGCAGC GCGGCCAGCC TGCTGACGCC CGGGGGAATC 6000
GCCACGTTCG TGCGCGGTGC
CGCTCATTCG ATAACCGCCG CGCGTGCCAG CGCGATTCTG 6060
GTAATGGGTT TCCCAGTGTT
GCTCAAAGTG ACCTCCGACC AGTTAGGGGC AAAGGGCGGA 6120
GCGGTCATCC TGGCTGTGAC
CTTGACGCGT GCGCCGCTTC TGGTCCCACT GAGCGCGATG 6180
CAAGGCAACC TGATCGCGCA
TTTCGTCGAC CGGCGCACCC AACGGCTTCG GGCGCTGATC 6240
GCACCGGCGC TGGTCGTCGG
CGGCATCGGT GCGGTCGGGA TGTTGGCCGC AGGGCTTACC 6300
GGTCCCTGGT TGCTGCGTGT
TGGATTCGGC CCCGACTACC AAACTGGCGG GGCGTTGCTG 6360
GCCTGGTTGA CGGCAGCGGC
GGTAGCTATC GCCATGCTGA CGCTGACCGG CGCCGCCGCG 6420
GTCGCGGCCG CACTGCACCG
GGCGTATTTG CTGGGCTGGG TCAGCGCGAC GGTGGCGTCG 6480
ACGCTGTTGC TGCTGCTGCC
GATGCCGCTG GAGACGCGCA CCGTGATCGC GCTGTTGTTC 6540
GGTCCAACGG TGGGAATCGC
CATCCATGTG GCCGCGTTGG CGCGGCGACC CGACTGATTT 6600
GTGCCCCAGG TCGACAAATC
ACGCCGTCTC GTCAGTGAGC ACTCCGTCCT CGGGTCCGAT 6660
CCTTCCAGGA GACGTTGCAA
CCTGATTTGG CTCAAATTGG TGCGCACCGA GGGTCGGGCA 6720
CATCGTAGGG TCGCAACAGT
CACATGTGTC ACTGCACCGG GCGACACCCG ATGTCCCGGC 6780
TCTCAGCGAC AGCTGTCTGA
CCTGTGGTTT TGTTCCCAAG TTGGTCGTGG CTGTGCGGGA 6840
TTGGAGGTGG CGTGGGGGTC
GCGTCGTATG GATTCTCCTC CTCGGTTCCG CGCGAAACGG 6900
CCGCAGGCGC AATGGTCACC
AACTTGGCCG CGGTGGAGTC TAGCCTCACA TTTTCCTGGT 6960
CGCCCCCGAC AACCAGGAGG
TCGCTGCAGA ACGGGCGTTC CCTACCCACA TCTACTATGA 7020
AGCGACAGCG GCGCCCCGCT
GTGATGGCTG AGCATGACCG ACAGAGGCGG GAAGACAGTG 7080
AAGCGAGCGC TCATCACCGG
AATCACCGGC CAGGACGGCT CGTATCTCGC CGAACTGCTG 7140
CTGGCCAAGG GGTATGAGGT
TCACGGGCTC ATCCGGCGCG CTTCGACGTT CAACACCTCG 7200
CGGATCGATC ACCTCTACGT
CGACCCGCAC CAACCGGGCG CGCGGCTGTT TCTGCACTAT 7260
GGTGACCTGA TCGACGGAAC
CCGGTTGGTG ACCCTGCTGA GCACCATCGA ACCCGACGAG 7320
GTGTACAACC TGGCGGCGCA
GTCACACGTG CGGGTGAGCT TCGACGAACC CGTGCACACC 7380
GGTGACACCA CCGGCATGGG
ATCCATGCGA CTGCTGGAAG CCGTTCGGCT CTCTCGGGTG 7440
CACTGCCGCT TCTATCAGGC
GTCCTCGTCG GAGATGTTCG GCGCCTCGCC GCCACCGCAG 7500
AACGAGCTGA CGCCGTTCTA
CCCGCGGTCA CCGTATGGCG CCGCCAAGGT CTATTCCTAC 7560
TGGGCGACCC GCAATTATCG
CGAAGCGTAC GGATTGTTCG CCGTTAACGG CATCTTGTTC 7620
AATCACGAAT CACCGCGGCG
CGGTGAGACG TTCGTGACCC GAAAGATCAC CAGGGCCGTG 7680
GCACGCATCA AGGCCGGTAT
CCAGTCCGAG GTCTATATGG GCAATCTGGA TGCGGTCCGC 7740
GACTGGGGGT ACGCGCCCGA
ATACGTCGAA GGCATGTGGC GGATGCTGCA GACCGACGAG 7800
CCCGACGACT TCGTTTTCGC
GACCGGGCGC GGTTTCACCG TGCGTGAGTT CGCGCGGGCC 7860
GCGTTCGAGC ATGCCGGTTT
GGACTGGCAG CAGTACGTGA AATTCGACCA ACGCTATCTG 7920
CGGCCCACCG AGGTGGATTC
GCTGATCGGC GACGCGACCA AGGCTGCCGA ATTGCTGGGC 7980
TGGAGGGCTT CGGTGCACAC
TGACGAGTTG GCTCGGATCA TGGTCGACGC GGACATGGCG 8040
GCGCTGGAGT GCGAAGGCAA
GCCGTGGATC GACAAGCCGA TGATCGCCGG CCGGACATGA 8100
ACGCGCACAC CTCGGTCGGC
CCGCTTGACC GCGCGGCCCG GGTCTACATC GCCGGGCATC 8160
GCGGCCTGGT CGGGTCCGCG
CTGCTACGCA CGTTTGCGGG CGCGGGGTTC ACCAACCTGC 8220
TGGTGCGGTC ACGCGCCGAG
CTTGATCTGA CGGATCGGGC CGCGACGTTC GACTTCGTTC 8280
TCGAGTCGAG GCCGCAGGTC
GTCATCGACG CGGCGGCCCG GGTCGGCGGC ATCCTGGCCA 8340
ACGACACCTA CCCGGCCGAT
TTCCTGTCGG AAAACCTCCA GATCCAGGTC AACCTGCTGG 8400
ATGCCGCCGT GGCGGCGCGG
GTGCCGCGGC TGCTGTTCCT GGGCTCGTCG TGCATCTACC 8460
CGAAACTCGC CCCGCAGCCG
ATCCCGGAGA GCGCGCTGCT CACCGGTCCG TTGGAGCCGA 8520
CCAACGACGC GTACGCGATC
GCCAAAATCG CCGGCATCCT TGCGGTCCAG GCGGTGCGCC 8580
GCCAACATGG CCTGCCGTGG
ATCTCGGCGA TGCCCACCAA CCTGTACGGG CCAGGCGACA 8640
ACTTTTCGCC GTCCGGCTCG
CATCTGCTGC CGGCACTCAT CCGCCGCTAT GACGAGGCCA 8700
AAGCCAGTGG CGCGCCCAAC
GTGACCAACT GGGGCACCGG CACGCCCCGA CGGGAGTTGC 8760
TGCACGTCGA CGACCTGGCG
AGCGCATGCC TGTATCTGCT GGAACATTTC CACGGGCCGA 8820
CCCATGTCAA CGTGGGAACC
GGCATCGACC ACACCATCGG CGAGATCGCC GAGATGGTCG 8880
CCTCGGCGGT AGGCTATAGC
GGCGAAACCC GCTGGGATCC AAGCAAACCG GACGGAACAC 8940
CACGCAAACT GCTGGATGTT
TCGGTGCTAC GGGAGGCGGG ATGGCGGCCT TCGATCGCGC 9000
TGCGCGACGG CATCGAGGCG
ACGGTGGCGT GGTATCGCGA GCACGCGGGA ACGGTTCGGC 9060
AATGAGGCTG GCCCGTCGCG
CTCGGAACAT CTTGCGTCGC AACGGCATCG ACGTGTCGCG 9120
CTACTTTGCC GAACTGGACT
GGGAACGCAA TTTCTTGCGC CAACTGCAAT CCCATCGGGT 9180
CAGTGCCGTG CTCGATGTCG
GGGCCAATTC GGGGCAGTAC GCCAGGGGTC TGCGCGGCGC 9240
GGGCTTCGCG GGCCGCATCG
TCTCGTTCGA GCCGCTGCCC GGGCCCTTTG CCGTCTTGCA 9300
GCGCAGCGCC TCCACGGACC
CGTTGTGGGA ATGCCGGCGC TGTGCGCTGG GCGATGTCGA 9360
TGGAACCATC TCGATCAACG
TCGCCGGCAA CGAGGGCGCC AGCAGTTCCG TCTTGCCGAT 9420
GTTGAAACGA CATCAGGACG
CCTTTCCACC AGCCAACTAC GTGGGCGCCC AACGGGTGCC 9480
GATACATCGA CTCGATTCCG
TGGCTGCAGA CGTTCTGCGG CCCAACGATA TTGCGTTCTT 9540
GAAGATCGAC GTTCAAGGAT
TCGAGAAGCA GGTGATCGCG GGTGGCGATT CAACGGTGCA 9600
CGACCGATGC GTCGGCATGC
AGCTCGAGCT GTCTTTCCAG CCGTTGTACG AGGGTGGCAT 9660
GCTCATCCGC GAGGCGCTCG
ATCTCGTGGA TTCGTTGGGC TTTACGCTCT CGGGATTGCA 9720
ACCCGGTTTC ACCGACCCCC
GCAACGGTCG AATGCTGCAG GCCGATGGCA TCTTCTTCCG 9780
GGGCAGCGAT TGACGCGCCG
GCGCGTCAAT CTATTTCGAC ATTCGCGTGA AGACGTTTTC 9840
CCAGAATCGA CTGTTGTAGG
CGTAGAACTC CCGGCCGCGT AGGTAGGCAT GTGATATTCG 9900
CCTTCCCCCG AACGGGTAGC
GGCGATGAAG GTCGCCCATG CGGCGCAGAT CACCGAAGAC 9960
CGCGCTTGGT TCCCGGTGCG
AGCCGACGCC CGTGGTGTCG AACTCGCACA GCACACACCG 10020
AATCGTGACC GGCTCGCATA
CCAGCGCGGC CCGCAATATG AATTCCTGGT CGGCGGCGAT 10080
CCCGAAATCA AGGTCGTAGC
CACCGATCTT GGCCACCAGC GATGATCCGA AGAACGATGC 10140
TTGATGCGGA ACAACCTGCT
TGCCGGCCAG GAATTTGCGC AGGCTGAAAG GTATCGGGCC 10200
GCGCACCCGA TCGAGCCCGA
CGAGACGATC CATCCCGAAG CCCCACAATT CGGACACCGG 10260
TCCCTTGCCG GATAGCGCCT
CCACGGCCTG GGOTACCACG TCGGGCCCGG AAAAACGATC 10320
GGCGGAGTGC AAGAACCACA
ACAGATCACC CGATGCGTGC GCGATGCCCT GGTTCATCGC 10380
GTCGTACCGC CCGCCGTCGG
GCTCGGACTG CCAATACGCG AAGCCTGGTT CACACCCGGA 10440
CAGGTATGCC ACCACGTCGT
CGCCGCTGCC ACCGTCGATT ACGATGTGCT CGATGCGTCC 10500
CCGGTAGCGT TGCGCCCGCA
CACTTTTCAC CGTGCGCTGC AACCCGTCGA GGTCGTTGAA 10560
CGAGATCGTT ATCACCGAGA
CGGTCGGAGC AGACGTCACC GAGTTCCCCT AGGTTGCTGG 10620
CGGCGATTGT GGATCACCGG
GTCTTGATAC CGATGAAGGT GCCTCGAAGA TTCGCCGCAT 10680
AGGAACCTCC GAGCAACGAC
TCGGCGATGC TTGGTTCCAA GTTGTCGTAC TCCTCCATCA 10740
CCAGGTCGAC GCCGACGTCT
TTGATGGCCT GAAGTAGGTG CTCGCGTTGA ATCCAGAATG 10800
ACCGGCGATT GTCCCAGGAC
GCCCATTTTG CGGTGTCGCG CTGGCCAAAC GAGCGGTCGT 10860
CGGAAAACTC GGTAAACCAC
CTACCGGGAA GTCCCTCATG TTCGGTGGGC GCCGAGAGCA 10920
TGAACTTCAC CGGCGCCGGC
CGCCGCAGCA ACCGATCGGT CAATTGTCGT GCCGTCGTGG 10980
GCAACCGGAG CCATTTATCG
CTCCGGTTGA TGATCGAGAA GTGCGTCTGG AGAATCAGCA 11040
GCTTGTTCGT TACCGACGAG
AGGGTTTCCA GGTATTGCTT CGGATTCTCC AGGTGGTAGA 11100
AGAGGCCGCA GCAGAAGACG
GTATCGAAGA GCCCGTGGTT GGCGATGTTG AGGGCGTTGT 11160
CGTGGACGAA CCGGAGATTC
GGCAGGTTGG TCTTCGATTT GATGTAGTTG CAGGCCGCCA 11220
TGTTCAGCTC GCGAACCTCG
ATCCCGAGGA CCTGAAATCC CATGCGCGCG AACCCGACCG 11280
CGTACCCGCC TTCCAAGCAG
CCGACATCGG CCAGGCGTAG GTGGCTCTTG TCCCCGGGAA 11340
AGACGGTTTC CAGAATCCCG
CGCGCCGAGA TGAACCAGGA CGATTCGTCT AACGTGCGCG 11400
AGGACTCCGG TATCGTCAAG
GTTCCGTCGT CGAGGCGAAC GTTGTGGGCG GTGAATTGTA 11460
CCGCGCCGGC CGAATGTTCC
TGTGCCATCA CTTGGTTAGC CCCTTCGGCT GGTCCTGGGT 11520
TTGTCGACAT GGTCAGGCTC
GACAGCCGCG TCGGAGCCGG GAGGGCCACA CATCCACGAG 11580
CCCCCTGCGG CTCGGCGTCG
CGGCGGCGAG CTTGCGCCAC TGGGTCTTGA GCCGCCGCGC 11640
GGGTGTCGCC CCGCGGTGCT
GCAGCGCCAG CATGGCGATC CGGGGATGGC GCGCGATGGT 11700
TTCCTGCAGC GCGGCGCGCC
CCTCCGGGCC TGGAACGTTG GCGATCTGGC GAAGGATCCA 11760
GTCGGCCATG ACGGCGATGA
GCTCCTCGCG CGCGGGGTCT CCCGGGAACA GGTCGAGCAT 11820
CGCGTCAAAC GTCGCCGCAT
GCCCCGGACC CTGCGTCAAC CAGAACTTTG GCGGGTCCAC 11880
CACCTGGTTG TGCCACATGC
CTTGGGCGTG GCGGCGATAC ACGGCCATGG TGTCGGGCAA 11940
CATGGCGATG TCGCCATGCA
CCGCGTGCCG GACGTGCAGA TACCAGTCCA GGGGCATGAC 12000
GTCGGCAGGA ATGTCGTCGT
AGCGCTCGAG GCGACGGTAC ACGGCCGAGT TGGTCTGGAT 12060
GAAGTTCATC AAGATCAACG
CATCCAGGCT CAAGTTGCCC CGCACCCGAA CCGGGGGGAA 12120
CTTCGAGTCC TTGGCATGGC
CGTCCTCCCA TATCACTCGG ACGGGATGGA AGCACACCGT 12180
CGTCTTGGGG TGCCGGTCGA
GGAATGCGAC CTGTTTGCTT AGCTTCAGCG GATCGATCCA 12240
GTAGTCGTCC GCCTCGCACA
ACGCGACGTA CTCGCCGCGA GCGGCCGACA GGGCGCCGGT 12300
CAGGTTCCCA TTGAGGCCGA
GGTTTTCGGT CCTGAAGATC GGCCGGAACA CGTGCGGGTA 12360
CCGCTCGGCG TACTCACGGA
TGATCGCCGG GGTGGCATCG GTCGACGCGT CGTCGGCGAC 12420
GATGATCTCC ACCGGGAAGT
CGGTTTGCTG GTCGAGAAAG CTGTCGAAGG CCTGACGGGC 12480
GTAGCCCGCC TGGTTGTGAG
TGGTCGAGAC GATGCTCACC TTGGGGCAAA GCTGGGGACT 12540
CACCGTCGGC CCTTTTCCTG
CGCGGCCGCA AGGGTATTGC GATGGCGAAC GTGAATCGCC 12600
TGTGCCCGCC GGCCGTCGGC
CGTCGTGGCC TGGTGGTCGG CGGACGTACG GCACACGCTG 12660
GCGAAGTATA GCGAGGGTGC
ACTGACGTTG GGCTCGAACC GCGTGGCGCG CGGTGTGGGC 12720
CCACCGTCTC GAGTCGGTGC
TGGTTGGCTC GC 12732
The location, on the Mycobacterium tuberculosis chromosome, of the above polynucleotide of sequence SEQ ID No1 has now been ascribed to begin, at its 5′end at nucleotide at position nt 1696015 and to end, at its 3′end, at nucleotide at position nt 1708746.
For diagnostic purposes, this 12.7 kb deletion should allow a rapid PCR screening of tubercle isolates to identify whether they are bovine or human strains. The primers listed in Table 1 are flanking the deleted region and give a 722 bp amplicon in M. bovis or M. bovis BCG strains, but a fragment of 13,453 bp in M. tuberculosis that is practically impossible to amplify under the same PCR conditions. More importantly, assuming that some of the gene products from this region represent proteins with antigenic properties, it could be possible to develop a test that can reliably distinguish between the immune response induced by vaccination with M. bovis BCG vaccine strains and infection with M. tuberculosis or that the products (e.g. polysaccharides) are specific immunogens.
The invention also provides for a purified polynucleotide useful as a probe or as a primer, said polynucleotide being chosen in the following group of polynucleotides:
- a) a polynucleotide comprising at least 8 consecutive nucleotides of the sequence SEQ ID No1.
- b) a polynucleotide whose sequence is fully complementary to the sequence of the polynucleotide defined in a);
- c) a polynucleotide that hybridizes under stringent hybridization conditions with the polynucleotide defined in a) or with the polynucleotide defined in b).
For the purpose of defining a polynucleotide or oligonucleotide hybridizing under stringent hybridization conditions, such as above, it is intended a polynucleotide that hybridizes with a reference polynucleotide under the following hybridization conditions:
The hybridization step is realized at 65° C. in the presence of 6×SSC buffer, 5× Denhardt's solution, 0.5% SDS and 100 μg/ml of salmon sperm DNA.
For technical information, 1×SSC corresponds to 0.15 M NaCl and 0.05M sodium citrate; 1× Denhardt's solution corresponds to 0.02% Ficoll, 0.02% polyvinylpyrrolidone and 0.02% bovine serum albumin.
The hybridization step is followed by four washing steps:
-
- two washings during 5 min, preferably at 65° C. in a 2×SSC and 0.1% SDS buffer;
- one washing during 30 min, preferably at 65° C. in a 2×SSC and 0.1% SDS buffer,
- one washing during 10 min, preferably at 65° C. in a 0.1×SSC and 0.1% SDS buffer
A first illustrative useful polynucleotide that is included in the polynucleotide of sequence SEQ ID No1 is the following polynucleotide of sequence SEQ ID No2 that corresponds to the Sp6 endsequence of SEQ ID No1:
ATACTCAAGC TTGCCGCAAT CGAAACCAAC CTGTTTGTGC 60
CGCAAGAAAT TACGCCGTGG
CCCGGCGCCG ATCAAGAAAC GCCCCGGCGC GCGGCGGTGT 120
CGTCGTATGG CATGACGGGC
ACCAATGTGC ACGCCATTGT CGAGCAGGCA CCGGTGCCAG 180
CCCCCGAATC CGGTGCACCA
GGCGACACCC CGGCCACACC CGGTATCGAC GGCGCGCTGC 240
TGTTCGCGCT GTCGGCCAGC
TCGCAGGACG CGCTGCGGCA AACCGCCGCG CGGCTGGCCG 289
ATTGGGTCT
A second illustrative useful polynucleotide that is included in the polynucleotide of sequence SEQ ID No1 is the following polynucleotide of sequence SEQ ID No3 that corresponds to the T7 endsequence of SEQ ID No1, located on the opposite strand:
TTGGCGGGTT GGCCACACAC CCGCCGGTGA CGGCGACGAT 60
GCTGGGCTGG TTGCGGCCCT
GCGCCACCGC GGCTTGCATG CTGGTTGGCT GTCTTGGGAC 120
GATCCCGAAA TAGTCCACGC
GGATCTGGTG ATTTTGCGGG CTACCCGCGA TTACCCCGCG 180
CGGCTCGACG AGTTTTTGGC
CTGGACTACC CGCGTGGCCA ATCTGCTGAA CTCGCGGCCG 240
GTGGTGGCCT GGAATGTCCA
CGCCGTTCAC CTACGTGACC TTGATGGGAT 278
CCGGGGGT
The polynucleotide of sequence SEQ ID No1 contains 11 ORFs, the respective locations of which, taking into account the orientation of each ORF on the chromosome, on the sequence of the Mycobacterium tuberculosis chromosome, is given hereafter:
-
- The location of ORF1 is comprised between nucleotide at position nt 1695944 and nucleotide at position nt1696441.
- The location of ORF2 is comprised between nucleotide at position nt 1696728 and nucleotide at position nt1697420.
- The location of ORF3 is comprised between nucleotide at position nt 1698096 and nucleotide at position nt1699892. ORF3 probably encodes a protein having the characteristics of a membrane protein.
- The location of ORF4 is comprised between nucleotide at position nt 1700210 and nucleotide at position nt1701088.
- The location of ORF5 is comprised between nucleotide at position nt 1701293 and nucleotide at position nt1702588. ORF5 encodes a protein having the characteristics of a membrane protein.
- The location of ORF6 is comprised between nucleotide at position nt 1703072 and nucleotide at position nt1704091. ORF6 encodes a protein having the characteristics of a GDP-D-mannose dehydratase.
- The location of ORF7 is comprised between nucleotide at position nt 1704091 and nucleotide at position nt1705056. ORF7 encodes a protein having the characteristics of a nucleotide sugar epimerase involved in colanic acid biosynthesis.
- The location of ORF8 is comprised between nucleotide at position nt 1705056 and nucleotide at position nt1705784.
- The location of ORF9 is comprised between nucleotide at position nt 1705808 and nucleotide at position nt1706593. ORF9 encodes a protein having the characteristics of colanic acid biosynthesis glycosyl transferase.
- The location of ORF10 is comprised between nucleotide at position nt 1706631 and nucleotide at position nt1707524.
The location of ORF11 is comprised between nucleotide at position nt 1707530 and nucleotide at position nt1708648. ORF11 encodes a protein similar to a spore coat polysaccharide biosynthesis.
A polynucleotide of interest obtained by the above-disclosed method according to the invention may also contain at least one ORF that encodes all or part of acidic, glycine-rich proteins, belonging to the PE and PPE families, whose genes are often clustered and based on multiple copies of the polymorphic repetitive sequences. The names PE and PPE derive from the fact that the motifs ProGlu (PE, positions 8, 9) and ProProGlu (PPE, positions 7 to 9) are found near the N-terminus in almost all cases. The PE protein family all have a highly conserved N-terminal domain of ˜110 amino acid residues, that is predicted to have a globular structure, followed by a C-terminal segment which varies in size, sequence and repeat copy number. Phylogenetic analysis separated the PE family into several groups, the larger of which is the highly repetitive PGRS class containing 55 members whereas the other groups share very limited sequence similarity in their C-terminal domains. The predicted molecular weights of the PE proteins vary considerably as a few members only contain the ˜110 amino acid N-terminal domain while the majority have C-terminal extensions ranging in size from 100 up to >1400 residues. A striking feature of the PGRS proteins is their exceptional glycine content (up to 50%) due to the presence of multiple tandem repetitions of GlyGlyAla or GlyGlyAsn motifs or variations thereof.
Like the PE family, the PPE protein family also has a conserved N-terminal domain that comprises ˜180 amino acid residues followed by C-terminal segments that vary considerably in sequence and length. These proteins fall into at least three groups, one of which constitutes the MPTR class characterised by the presence of multiple, tandem copies of the motif AsnXGlyXGlyAsnXGly. The second subgroup contains a characteristic, well-conserved motif around position 350 (GlyXXSerValProXXTrp), whereas the other group contains proteins that are unrelated except for the presence of the common 180-residue PPE domain. C-terminal extensions may range in size from 00 up to 3500 residues.
One member of the PGRS sub-family, the WHO antigen 22T (Abou-Zeid et al., 1991), a 55 kD protein capable of binding fibronectin, is produced during disease and elicits a variable antibody response suggesting either that individuals mount different immune responses or that this PGRS-protein may not be produced in this form by all strains of M. tuberculosis. In other words, at least some PE_PGRS coding sequences encode for proteins that are involved in the recognition of M. tuberculosis by the immune system of the infected host. Therefore, differences in the PGRS sequences could represent the principal source of antigenic variation in the otherwise genetically and antigenically homogeneous bacterium.
By performing the method of the invention using the M tuberculosis BAC based DNA library I-1945, the inventors have discovered the occurence of sequence differences between a given PGRS encoding ORF (ORF reference on the genomic sequence of M. tuberculosis Rv0746) of M. tuberculosis and its counterpart sequence in the genome of M. bovis BCG.
More precisely, the inventors have determined that one ORF contained in BAC vector No Rv418 of the M. tuberculosis BCG I-1945 DNA library carries both base additions and base deletions when compared with the corresponding ORF in the genome of M. bovis BCG that is contained in the BAC vector No X0175 of the M. bovis BCG I-XXXX DNA libary. The variations observed in the base sequences correspond to variations in the C-terminal part of the aminoacid sequence of the PGRS ORF translation product.
As shown in FIG. 6, an amino acid stretch of 9 residues in length is present in this M. tuberculosis PGRS (ORf reference Rv0746) and is absent from the ORF counterpart of M. bovis BCG, namely the following amino acid sequence:
NH2-GGAGGAGGSSAGGGGAGGAGGAGGWLLGD-COOH.
Furthermore, FIG. 6 shows also that an amino acid stretch of 45 residues in length is absent from this M. tuberculosis PGRS and is present in the ORF counterpart of M. bovis BCG, namely following amino acid sequence:
NH2-GAGGIGGIGGNANGGAGGNGGTGGQLWGSGGAGVEGGAALSVGDT-
COOH.
Similar observations were made with PPE ORF Rv0442, which showed a 5 codon deletion relative to a M. bovis amino acid sequence.
Given that the polymorphism associated with the PE-PGRS or PEE ORFS resulted in extensive antigenic variability or reduced antigen presentation, this would be of immense significance for vaccine design, for understanding protective immunity in tuberculosis and, possibly, explain the varied responses seen in different BCG vaccination programmes.
There are several striking parallels between the PGRS proteins and the Epstein-Barr virus-encoded nuclear antigens (EBNA). Both polypeptide families are glycine-rich, contain Gly-Ala repeats that represent more than one third of the molecule, and display variation in the length of the repeat region between different isolates. The Gly-Ala repeat region of EBNA1 has been shown to function as a cis-acting inhibitor of antigen processing and MHC class I-restricted antigen presentation (Levitskaya et al., 1995). The fact that MHC class I knock-out mice are extremely suscepible to M. tuberculosis underlines the importance of MHC class I antigen presentation in protection against tuberculosis. Therefore, it is possible that the PE/PPE protein family also play some role in inhibiting antigen presentation, allowing the bacillus to hide from the host's immune system.
As such the novel and nonobvious PGRS polynucleotide from M. bovis which is homolog to the M. tuberculosis ORF Rv0746, and which is contained in the BAC clone No X0175 (See Table 4 for SP6 and T7 endsequences of clone no X0175) of the I-XXXX M. bovis BCG BAC DNA library is part of the present invention, as it represents a starting material in order to define specific probes or primers useful for detection of antigenic variability in mycobacterial strains, possible inhibition of antigen processing as well as to differentiate M. tuberculosis from M. bovis BCG.
Thus, a further object of the invention consists in a polynucleotide comprising the following sequence SEQ ID No4:
CCGACCCAGA CACTGACCGG GCGACCGCTG ATCGGCAACG 60
GCACCCCCGG GGCGGTCCGC
AGCGGGGCCA CCGGGGCCCC CGGTGGGTGG CTGCTCGGCG 120
ACGGCGGGGC CGGCGGGTCC
GGCGCGGCGG GCTCGGGCGC GCCCGGCGGG GCGGGCGGGG 180
CTGCCGGGCT GTGGGGTACC
GGCGGGGCCG GCGGGATCGG CGGAGCCAGC ACCGTACTCG 240
GCGGCACCGG CGGGGGAGGC
GGGGTCGGTG GGCTGTGGGG CGCCGGTGGG GCCGGCGGGG 300
CCGGTGGAAC CGGCCTTGTT
GGTGGCGACG GCGGGGCCGG TGGGGCCGGC GGGACCGGCG 360
GACTGCTGGC CGGGCTGATC
GGTGCCGGCG GAGGTCACGG CGGGACCGGC GGGCTCAGCA 420
CTAATGGCGA CGGCGGGGTT
GGCGGGGCCG GCGGGAATGC CGGAATGCTC GCCGGGCCGG 480
GCGGCGCCGG CGGAGCCGGC
GGTGACGGCG AAAACCTGGA CACCGGTGGG GACGGCGGGG 540
CCGGCGGTAG CGCAGGGCTG
CTGTTCGGCA GCGGCGGCGC CGGCGGCGCC GGCGGATTTG 600
GTTTCCTCGG TGGGGACGGC
GGGGCCGGTG GCAACGCCGG GCTGCTGTTG TCCAGCGGCG 660
GGGCCGGCGG GTTCGGCGGG
TTCGGCACCG CCGGTGGGGT CGGTGGGGCC GGCGGCAATG 720
CCGGCTGGCT GGGCTTCGGC
GGGGCCGGGG GCATCGGCGG AATCGGCGGT AACGCTAACG 780
GGGGCGCCGG TGGGAACGGC
GGCACCGGCG GTCAGTTATG GGGTAGCGGC GGCGCCGGCG 840
TCGAAGGCGG CGCAGCCTTA
AGCGTCGGCG ACACCGGCGG GGCCGGTGGC GTCGGCGGCA 900
GCGCCGGGCT GATCGGCACC
GGCGGCAACG GCGGCAACGG CGGCACCGGC GCCAACGCCG 960
GCAGCCCCGG AACCGGCGGC
GCCGGCGGGT TGCTGCTGGG CCAAAACGGG CTCAACGGGT 1020
TGCCGTAGCC GGGCGGCACG
GCATGGCTTC CGGGCGTCAA CCACTCGCCG GTGATGCAGA 1080
TCGGCTGCGG AGCGGGCCGC
CAAAATGGGG GCCGCCGCGC CAGGTATCTC GGCGAAGATC 1140
CCCGGCGCTC GAGCGCTTTG
TCAGAGGCCC GTCGCGGGTC GTCGTGACGA CGGCTATCCG 1200
GGCGGTGCGG GTTTCGCGGC
GCGCCCTGTG CCCGGCACCG CCGCCCGTTT GTCGGCAACG 1260
CCGCCGCGAC CCGTGAGCCG
TCCAGCAGCT GGCGCCTGCG 1280
Polynucleotides of interest have been defined by the inventors as useful detection tools in order to differentiate M. tuberculosis from M. bovis BCG. Such polynucleotides are contained in the 45 aminoacid length coding sequence that is present in M. bovis BCG but absent from M. tuberculosis. This polynucleotide has a sequence beginning (5′end) at the nucleotide at position nt 729 of the sequence SEQ ID No4 and ending (3′end) at the nucleotide in position nt 863 of the sequence SEQ ID No4.
Thus, part of the present invention is also a polynucleotide which is chosen among the following group of polynucleotides:
a) A polynucleotide comprising at least 8 consecutive nucleotides the following nucleotide sequence SEQ ID No5:
GGGCATCGGC GGAATCGGCG GTAACGCTAA CGGGGGCGCC 60
GGTGGGAACG GCGGCACCGG
CGGTCAGTTA TGGGGTAGCG GCGGCGCCGG CGTCGAAGGC 120
GGCGCAGCCT TAAGCGTCGG
CGACACC 127
- b) A polynucleotide which sequence is fully complementary to the sequence of the polynucleotide defined in a);
- c) A polynucleotide that hybridizes under stringent hybridization conditions with the polynucleotide defined in a) or with the polynucleotide defined in b).
The stringent hybridization conditions for the purpose of defining the above disclosed polynucleotide are defined herein before in the specification.
The invention also provides for a BAC-based Mycobacterium tuberculosis strain H37Rv genomic DNA library that has been deposited in the Collection Nationale de Cultures de Microorganismes on Nov. 19, 1997 under the accession number I-1945.
A further object of the invention consists in a recombinant BAC vector which is choosen among the group consisting of the recombinant BAC vectors belonging to the BAC-based DNA library I-1945.
Generally, a recombinant BAC vector of interest may be choosen among the following set or group of BAC vectors contained in the BAC-based DNA library I-1945:
Rv101; Rv102; Rv103; Rv104; Rv105; Rv106; Rv107; Rv108; Rv109; Rv10; Rv110; Rv111; Rv112; Rv113; Rv114; Rv115; Rv116; Rv117; Rv118; Rv119; Rv11; Rv120; Rv121; Rv122; Rv123; Rv124; Rv126; Rv127; Rv128; Rv129; Rv130; Rv132; Rv134; Rv135; Rv136; Rv137; Rv138; Rv139; Rv13; Rv140; Rv141; Rv142; Rv143; Rv144; Rv145; Rv146; Rv147; Rv148; Rv149; Rv14; Rv150; Rv151; Rv152; Rv153; Rv154; Rv155; Rv156; Rv157; Rv159; Rv15; Rv160; Rv161; Rv162; Rv163; Rv164; Rv165; Rv166; Rv167; Rv169; Rv16; Rv170; Rv171; Rv172; Rv173; Rv174; Rv175; Rv176; Rv177; Rv178; Rv179; Rv17; Rv180; Rv181; Rv182; Rv183; Rv184; Rv185; Rv186; Rv187; Rv188; Rv18; Rv190; Rv191; Rv192; Rv193; Rv194; Rv195; Rv196; Rv19; Rv1; Rv201; Rv204; Rv205; Rv207; Rv209; Rv20; Rv214; Rv215; Rv217; Rv218; Rv219; Rv21; Rv220; Rv221; Rv222; Rv223; Rv224; Rv225; Rv226; Rv227; Rv228; Rv229; Rv22; Rv230; Rv231; Rv232; Rv233; Rv234; Rv235; Rv237; Rv240; Rv241; Rv243; Rv244; Rv245; Rv246; Rv247; Rv249; Rv24; Rv251; Rv252; Rv253; Rv254; Rv255; Rv257; Rv258; Rv259; Rv25; Rv260; Rv261; Rv262; Rv263; Rv264; Rv265; Rv266; Rv267; Rv268; Rv269; Rv26; Rv270; Rv271; Rv272; Rv273; Rv274; Rv275; Rv276; Rv277; Rv278; Rv279; Rv27; Rv280; Rv281; Rv282; Rv283; Rv284; Rv285; Rv286; Rv287; Rv288; Rv289; Rv28; Rv290; Rv291; Rv292; Rv293; Rv294; Rv295; Rv296; Rv29; Rv2; Rv301; Rv302; Rv303; Rv304; Rv306; Rv307; Rv308; Rv309; Rv30; Rv310; Rv311; Rv312; Rv313; Rv314; Rv315; Rv316; Rv317; Rv318; Rv319; Rv31; Rv32; Rv322; Rv327; Rv328; Rv329; Rv32; Rv330; Rv331; Rv333; Rv334; Rv335; Rv336; Rv337; Rv338; Rv339; Rv33; Rv340; Rv341; Rv343; Rv344; Rv346; Rv347; Rv348; Rv349; Rv34; Rv350; Rv351; Rv352; Rv353; Rv354; Rv355; Rv356; Rv357; Rv358; Rv359; Rv35; Rv360; Rv361; Rv363; Rv364; Rv365; Rv366; Rv367; Rv368; Rv369; Rv36; Rv370; Rv371; Rv373; Rv374; Rv375; Rv376; Rv377; Rv378; Rv379; Rv37; Rv381; Rv382; Rv383; Rv384; Rv385; Rv386; Rv387; Rv388; Rv389; Rv38; Rv390; Rv391; Rv392; Rv393; Rv396; Rv39; Rv3; Rv40; Rv412; Rv413; Rv414; Rv415; Rv416; Rv417; Rv418; Rv419; Rv41; Rv42; Rv43; Rv44; Rv45; Rv46; Rv47; Rv48; Rv49; Rv4; Rv50; Rv51; Rv52; Rv53; Rv54; Rv55; Rv56; Rv57; Rv58; Rv59; Rv5; Rv60; Rv61; Rv62; Rv63; Rv64; Rv65; Rv66; Rv67; Rv68; Rv69; Rv6; Rv70; Rv71; Rv72; Rv73; Rv74; Rv75; Rv76; Rv77; Rv78; Rv79; Rv7; Rv80; Rv81; Rv82; Rv83; Rv84; Rv85; Rv86; Rv87; Rv88; Rv89; Rv8; Rv90; Rv91; Rv92; Rv94; Rv95; Rv96; Rv9.
The end sequences of the polynucleotide inserts of each of the above clones corresponding respectively to the sequences adjacent to the T7 promoter and to the Sp6 promoter on the BAC vector are shown in Table 3.
It has been shown by the inventors that the minimal overlapping set of BAC vectors of the BAC-based DNA library I-1945 contains 68 unique BAC clones and practically spans almost the whole H37Rv chromosome with the exception of a single gap of approximately 150 kb.
More specifically, a recombinant BAC vector of interest is choosen among the following set or group of BAC vectors from the BAC-based DNA library I-1945, the location of which vector DNA inserts on the chromosome of M. tuberculosis is shown in FIG. 3:
Rv234; Rv351; Rv166; Rv35; Rv415; Rv404; Rv209; Rv272; Rv30; Rv228; Rv233; Rb38; Rv280; Rv177; Rv48; Rv374; Rv151; Rv238; Rv156; Rv92; Rv3; Rv403; Rv322; Rv243; Rv330; Rv285; Rv233; Rv219; Rv416; Rv67; Rv222; Rv149; Rv279; Rv87; Rv273; Rv266; Rv25; Rv136; Rv414; Rv13; Rv289; Rv60; Rv104; Rv5; Rv165; Rv215; Rv329; Rv240; Rv19; Rv74; Rv411; Rv167; Rv56; Rv80; Rv164; Rv59; Rv313; Rv265; Rv308; Rv220; Rv258; Rv339; Rv121; Rv419; Rv418; Rv45; Rv217; Rv134; Rv17; Rv103; Rv21; Rv22; Rv2; Rv270; Rv267; Rv174; Rv257; Rv44; Rv71; Rv7; Rv27; Rv191; Rv230; Rv128; Rv407; Rv106; Rv39; Rv255; Rv74; Rv355; Rv268; Rv58; Rv173; Rv264; Rv417; Rv401; Rv144; Rv302; Rv81; Rv163; Rv281; Rv221; Rv420; Rv175; Rv86; Rv412; Rv73; Rv269; Rv214; Rv287; Rv42; Rv143.
The polynucleotides disclosed in Table 3 may be used as probes in order to select a given clone of the BAC DNA library I-1945 for further use.
The invention also provides for a BAC-based Mycobacterium bovis strain Pasteur genomic DNA library that has been deposited in the Collection Nationale de Cultures de Microorganismes on XXXX XX, 1998 under the accession number I-XXXX.
A further object of the invention consists in a recombinant BAC vector which is chosen among the group consisting of the recombinant BAC vectors belonging to the BAC-based DNA library I-XXXX. This DNA library contains approximately 1600 clones. The average insert size is estimated to be ˜80 kb.
Generally, a recombinant BAC vector of interest may be chosen among the following set or group of BAC vectors contained in the BAC-based DNA library I-XXXX:
X0001; X0002; X0003; X0004; X0006; X0007; X0008; X0009; X0010; X0012; X0013; X0014; X0015; X0016; X0017; X0018; X0019; X0020; X0021; X0175.
The end sequences of the polynucleotide inserts of each of the above clones corresponding respectively to the sequences adjacent to the T7 promoter and to the Sp6 promoter on the BAC vector are shown in Table 4.
The polynucleotides disclosed in Table 4 may be used as probes in order to select a given clone of the BAC DNA library I-XXXX for further use.
Are also part of the invention the polynucleotide inserts that are contained in the above described BAC vectors, that are useful as primers or probes.
These polynucleotides and nucleic acid fragments may be used as primers for use in amplification reactions, or as nucleic probes.
PCR is described in the U.S. Pat. No. 4,683,202. The amplified fragments may be identified by an agarose or a polyacrylamide gel electrophoresis, or by a capillary electrophoresis or alternatively by a chromatography technique (gel filtration, hydrophobic chromatography or ion exchange chromatography). The specificity of the amplification may be ensured by a molecular hybridization using, for example, one of the initial primers as nucleic probes.
Amplified nucleotide fragments are used as probes in hybridization reactions in order to detect the presence of one polynucleotide according to the present invention or in order to detect mutations in the genome of the given mycobacterium of interest, specifically a mycobacterium belonging to the Mycobacterium tuberculosis complex and more specifically Mycobacterium tuberculosis and Mycobacterium bovis BCG.
Are also part of the present invention the amplified nucleic fragments (amplicons) defined herein above.
These probes and amplicons may be radioactively or non-radioactively labeled, using for example enzymes or fluorescent compounds.
Other techniques related to nucleic acid amplification may also be used and are generally preferred to the PCR technique.
The Strand Displacement Amplification (SDA) technique (Walker et al., 1992) is an isothermal amplification technique based on the ability of a restriction enzyme to cleave one of the strands at his recogntion site (which is under a hemiphosphorothioate form) and on the property of a DNA polymerase to initiate the synthesis of a new strand from the 3′OH end generated by the restriction enzyme and on the property of this DNA polymerase to displace the previously synthesized strand being localized downstream. The SDA method comprises two main steps:
- a) The synthesis, in the presence of dCTP-alpha-S, of DNA molecules that are flanked by the restriction sites that may be cleaved by an appropriate enzyme.
- b) The exponential amplification of these DNA molecules modified as such, by ezyme cleavage, strand displacement and copying of the displaced strands. The steps of cleavage, strand displacement and copy are repeated a sufficient number of times in order to obtain an accurate sensitivity of the assay.
The SDA technique was initially realized using the restriction endonuclease HincII but is now generally practised with an endonuclease from Bacillus stearothermophilus (BSOBI) and a fragment of a DNA polymerase which is devoid of any 5′→3′exonuclease activity isolated from Bacilllus cladotenax (exo-Bca) [=exo-minus-Bca]. Both enzymes are able to operate at 60° C. and the system is now optimized in order to allow the use of dUTP and the decontamination by UDG. When unsing this technique, as described by Spargo et al. in 1996, the doubling time of the target DNA is of 26 seconds and the amplification rate is of 1010 after an incubation time of 15 min at 60° C.
The SDA amplification technique is more easy to perform than PCR (a single thermostated waterbath device is necessary) and is faster thant the other amplification methods.
Thus, another object of the present invention consists in using the nucleic acid fragments according to the invention (primers) in a method of DNA or RNA amplification according to the SDA technique. For performing SDA, two pairs of primers are used: a pair of external primers (B1, B2) consisting of a sequence specific for the target polynucleotide of interest and a pair of internal primers (S1, S2) consisting of a fusion oligonucleotide carrying a site that is recognized by a restriction endonuclease, for exemple the enzyme BSOBI.
The operating conditions to perform SDA with such primers are described in Spargo et al. 1996.
The polynucleotides of the invention and their above described fragments, especially the primers according to the invention, are useful as technical means for performing different target nucleic acid amplification methods such as:
-
- TAS (Transcription-based Amplification System), described by Kwoh et al. in 1989;
- SR (Self-Sustained Sequence Replication), described by Guatelli et al. in 1990.
- NASBA (Nucleic acid Sequence Based Amplification), described by Kievitis et al. in 1991.
- TMA (Transcription Mediated Amplification).
The polynucleotides according to the invention are also useful as technical means for performing methods for amplification or modification of a nucleic acid used as a probe, such as:
-
- LCR (Ligase Chain Reaction), described by Landegren et al. in 1988 and improved by Barany et al. in 1991 who employ a thermostable ligase.
- RCR (Repair Chain Reaction) described by Segev et al. in 1992.
- CPR (Cycling Probe Reaction), described by Duck et al. in 1990.
- Q-beta replicase reaction, described by Miele et al. in 1983 and improved by Chu et al. in 1986, Lizardi et al. in 1988 and by Burg et al. and Stone et al. in 1996.
When the target polynucleotide to be detected is a RNA, for example a mRNA, a reverse transcriptase enzyme will be used before the amplification reaction in order to obtain a cDNA from the RNA contained in the biological sample. The generated cDNA is subsequently used as the nucleic acid target for the primers or the probes used in an amplification process or a detection process according to the present invention.
The non-labeled polynucleotides or oligonucleotides of the invention may be directly used as probes. Nevertheless, the polynucleotides or oligonucleotides are generally labeled with a radioactive element (32P, 35S, 3H, 125I) or by a non-isotopic molecule (for example, biotin, acetylaminofluorene, digoxigenin, 5-bromodesoxyuridin, fluorescein) in order to generate probes that are useful for numerous applications.
Examples of non-radioactive labeling of nucleic acid fragments are described in the french patent No FR-7810975 or by Urdea et al. or Sanchez-Pescador et al., 1988.
In the latter case, other labeling techniques may be also used such as those described in the french patents FR-2,422,956 and 2,518,755. The hybridization step may be performed in diffrent ways (Matthews et al., 1988). The more general method consists of immobilizing the nucleic acid that has been extracted from the biological sample onto a substrate (nitrocellulose, nylon, polystyrene) and then to incubate, in defined conditions, the target nucleic acid with the probe. Subsequently to the hybridization step, the excess amount of the specific probe is discarded and the hybrid molecules formed are detected by an appropriate method (radioactivity, fluorescence or enzyme activity measurement).
Advantageously, the probes according to the present invention may have structural characteristics such that they allow the signal amplification, such structural characteristics being, for example, branched DNA probes as those described by Urdea et al. in 1991 or in the European patent No EP-0225,807 (Chiron). In another advantageous embodiment of the probes according to the present invention, the latters may be used as capture probes, and are for this purpose immobilized on a substrate in order to capture the target nucleic acid contained in a biological sample. The captured target nucleic acid is subsequently detected with a second probe which recognizes a sequence of the target nucleic acid which is different from the sequence recognized by the capture probe.
The oligonucleotide probes according to the present invention may also be used in a detection device comprising a matrix library of probes immobilized on a substrate, the sequence of each probe of a given length being localized in a shift of one or several bases, one from the other, each probe of the matrix library thus being complementary to a distinct sequence of the target nucleic acid. Optionally, the substrate of the matrix may be a material able to act as an electron donor, the detection of the matrix poisitons in which an hybridization has occurred being subsequently determined by an electronic device. Such matrix libraries of probes and methods of specific detection of a targer nucleic acid is described in the European patent application No EP-0713,016 (Affymax technologies) and also in the U.S. Pat. No. 5,202,231 (Drmanac).
Since almost the whole length of a mycobacterial chromososme is covered by a BAC-based genomic DNA libraries according to the present invention (i.e. 97% of the M. tuberculosis chromosome is covered by the BAC library I-1945), these DNA libraries will play an important role in a plurality of post-genomic applications, such as in mycobacterial gene expression studies where the canonical set of BACs could be used as a matrix for hybridization studies. Probing such matrices with cDNA probes prepared from total mRNA will uncover genetic loci induced or repressed under different physiological conditions (Chuang et al., 1993; Trieselmann et al., 1992). As such, the H37Rv BAC library represents a fundamental resource for present and future genomics investigations.
The BAC vectors or the polynucleotide inserts contained therein may be directly used as probes, for example when immobilized on a substrate such as described herein before.
The BAC vectors or their polynucleotide inserts may be directly asdorbed on a nitrocellulose membrane, at predetermined locations on which one or several polynucleotides to be tested are then put to hybridize therewith.
Preferably, a collection of BAC vectors that spans the whole genome of the mycobacterium under testing will be immobilized, such as, for example, the set of 68 BAC vectors of the I-1945 DNA library that is described elsewhere in the specification and shown in FIG. 3.
The immobilization and hybridization steps may be performed as described in the present Materials and Methods Section.
As another illustrative embodiment of the use of the BAC vectors of the invention as polynucleotide probes, these vectors may be useful to perform a transcriptional activity analysis of mycobacteria growing in different environmental conditions, for example under conditions in which a stress response is expected, as it is the case at an elevated temperature, for example 40° C.
In this specific embodiment of the invention, Genescreen membranes may be used to immobilize the restriction endonuclease digests (HindIII digests for the BAC DNA library I-1945) of the BAC vectors by tranfer from a gel (Trieselmann et al., 1992).
Alternatively, the BAC vectors may be immobilized for dot blot experiments as follows. First, the DNA concentration of each BAC clone is determined by hybridization of blots of clone DNAs and of a BAC vector concentration standard with a BAC vector specific DNA probe. Hybridization is quantified by the Betascope 603 blot analyzer (Betagen Corp.), which colects beta particles directly from the blot with high efficiency. Then, 0.5 μg of each clone DNA is incubated in 0.25 M NaOH and 10 mM EDTA at 65° C. for 60 min to denature the DNA and degrade residual RNA contaminants. By using a manifold filtration system (21 by 21 wells), each clone DNA is blotted onto a GeneScreen Plus nylon membrane in the alkaline solution. After neutralization, the blots are baked at 85° C. for 2 h under vacuum. Positive and negative controls are added when necessary. In order to perform this procedure, it may be referred to the article of Chuang et al. (1993).
For RNA extractions, cells grown in a suitable volume of culture medium may, for example, be immediately mixed with an equal volume of crushed ice at −70° C. and spun at 4° C. in a 50 ml centrifugation tube. The cell pellet is then suspended in 0.6 ml of ice-cold buffer (10 mM KCl, 5 mM MgCl, 10 mM Tris; pH 7.4) and then immediately added to 0.6 ml of hot lysis buffer (0.4 M NaCl, 40 mM EDTA, 1% beta-mercaptoethanol 1% SDS, 20 mM Tris; pH 7.4) containing 100 μl of water saturated phenol. This mixture is incubated in a boiling water bath for 40 s. The debris are removed by centrifugation. The supernatant is extracted with phenol-chloroform five times, ethanol precipitated, and dried. The dried RNA pellet is dissolved in water before use.
Then labeled total cDNA may be prepared by the following method. The reaction mixture contains 15 μg of the previously prepared total RNA, 5 μg of pd(N6) (random hexamers from Pharmacia Inc.), 0.5 mM dATP, 0.5 mM dGTP and 0.5 mM DTTP, 5 μM dCTP, 100 μCi of [α-32P]dCTP (3,000 Ci/mmol), 50 mM Tris-HCl (pH 8.3), 6 mM MgCl2, 40 mM Kcl, 0.5 U of avian myeloblastosis virus reverse transcriptase (Life Science Inc.) in a total volume of 50 μl. The reaction is allowed to continue overnight at room temperature. EDTA and NaOH are then added to final concentrations of 50 mM and 0.25 M, respectively, and the mixture is incubated at 65° C. for 30 min to degrade the RNA templates. The cDNA is then ready to use after neutralization by adding Hcl and Tris buffer.
The hybridization step may be performed as described by Chuang et al. (1993) and briefly disclosed hereinafter. The DNA dot blot is hybridized to 32P-labeled total cDNA in a solution containing 0.1% polyvinylpyrrolidone, 0.1% Ficoll, 0.1% sodium Ppi, 0.1% bovine serum albumin, 0.5% SDS, 100 mM NaCl, and 0.1 mM sodium citrate, pH 7.2, at 65° C. for 2 days and then washed with a solution containing 0.1% SDS, 100 mM NaCl, and 10 mM Na-citrate, pH 7.2. The same dot blot is used for hybridization with both control and experimental cDNAs, with an alkaline probe stripping procedure (soaked twice in 0.25M NaOH-0.75 M NaCl at room temperature, 30 min each, neutralized, and completely dried at 65° C. for at least 30 min) between the two hybridizations. Quantification may be done with the Betascope 603 blot analyzer (Betagen Corp.).
As it flows from the above technical teachings, another object of the invention consists in a method for detecting the presence of mycobateria in a biological sample comprising the steps of:
- a) bringing into contact the recombinant BAC vector or a purified polynucleotide according to the invention with a biological sample.
- b) detecting the hybrid nucleic acid molecule formed between said purified polynucleotide and the nucleic acid molecules contained within the biological sample.
The invention further deals with a method for detecting the presence of mycobacteria in a biological sample comprising the steps of:
- a) Bringing into contact the recombinant BAC vector or a purified polynucleotide according to the invention that has been immobilized onto a substrate with a biological sample.
- b) Bringing into contact the hybrid nucleic acid molecule formed between said purified polynucleotide and the nucleic acid contained in the biological sample with a labeled recombinant BAC vector or a polynucleotide according to the invention, provided that said polynucleotide and polynucleotide of step a) have non-overlapping sequences.
Another object of the invention consists in a method for detecting the presence of mycobacteria in a biological sample comprising the steps of:
- a) Bringing into contact the nucleic acid molecules contained in the biological sample with a pair of primers according to the invention.
- b) Amplifying said nucleic acid molecules;
- d) detecting the nucleic acid fragments that have been amplified, for example by gel electrophoresis or with a labeled polynucleotide according to the invention.
In one specific embodiment of the above detection and/or amplification methods, said methods comprise an additional step wherein before step a), the nucleic acid molecules of the biological sample have been made available to a hybridization reaction.
In another specific embodiment of the above detection methods, said methods comprise an additional step, wherein, before the detection step, the nucleic acid molecules that are not hybridized with the immobilized purified polynucleotide are removed.
Also part of the invention is a kit for detecting mycobacteria in a biological sample comprising:
- a) A recombinant BAC vector or a purified polynucleotide according to the invention;
- b) Reagents necessary to perform a nucleic acid hybridization reaction.
The invention also pertains to a kit for detecting a mycobacteria in a biological sample comprising:
- a) A recombinant BAC vector or a purified polynucleotide according to the invention that is immobilized onto a substrate.
- b) Reagents necessary to perform a nucleic acid hybridization reaction.
- c) A purified polynucleotide according to the invention which is radioactively or non-radioactively labeled, provided that said polynucleotide and the polynucleotide of step a) have non-overlapping sequences.
Moreover, the invention provides for a kit for detecting mycobacteria in a biological sample comprising:
- a) A pair of purified primers according to the invention;
- b) Reagents necessary to perform a nucleic acid amplification reaction;
- c) Optionally, a purified polynucleotide according to the invention useful as a probe.
The invention embraces also a method for detecting the presence of a genomic DNA, a cDNA or a mRNA of mycobacteria in a biological sample, comprising the steps of:
- a) Bringing into contact the biological sample with a plurality of BAC vectors according to the invention or purified polynucleotides according to the invention, that are immobilized on a substrate;
- b) Detecting the hybrid complexes formed.
The invention also provides a kit for detecting the presence of genomic DNA, cDNA or mRNA of a mycobacterium in a biological sample, comprising:
- a) A substrate on which a plurality of BAC vectors according to the invention or purified polynucleotides according to the invention have been immobilized;
- b) Optionally, the reagents necessary to perform the hybridization reaction.
Additionally, the recombinant BAC vectors according to the invention and the polynucleotide inserts contained therein may be used for performing detection methods based on molecular combing. Said methods consist in methods for aligning macromolecules, especially DNA and are applied to processes for detecting, for measuring intramolecular distance, for separating and/or for assaying a macromolecule, especially DNA in a sample.
These molecular combing methods are simple methods, where the triple line S/A/B (meniscus) resulting form the contact between a solvent A and the surface S and a medium B is caused to move on the said surface S, the said macromolecules (i.e. DNA) having a part, especially an end, anchored on the surface S, the other part, especially the other end, being in solution in the solvent A. These methods are particularly fully described in the PCT Application No. PCT/FR 95/00165 files on Feb. 11, 1994 (Bensimon et al.).
When performing the molecular combing method with the recombinant BAC vectors according to the inventions or their polynucleotide inserts, the latters may be immobilized (anchored) on a suitable substrate and aligned as described in the PCT Application No. PCT/FR 95/00165, the whole teachings of this PCT Application being herien incorporated by reference. Then, polynucleotides to be tested, preferably under the form of radioactively or non radioactively labeled polynucleotides, that may consist of fragments of genomic DNA, cDNA etc. are brought into contact with the previously aligned polynucleotides according to the present invention and then their hybridization position on the aligned DNA molecules is determined using any suitable means including a microscope or a suitable camera device.
Thus, the present invention is also directed to a method for the detection of the presence of a polynucleotide of mycobacterial origin in a biological sample and/or for physical mapping of a polynucleotide on a genomic DNA, said method comprising:
- a) Aligning at least one polynucleotide contained in a recombinant BAC vector according to the invention on the surface of a substrate;
- b) Bringing into contact at least one polynucleotide to be tested with the substrate on which the at least one polynucleotide of step a) has been aligned;
- c) detecting the presence and/or the location of the tested polynucleotide on the at least one aligned polynucleotide of step a).
The invention finally provides for a kit for performing the above method, comprising:
- a) a substrate whose surface has at least one polynucleotide contained in a recombinant BAC vector according to the invention;
- b) optionally, reagents necessary for labeling DNA;
- c) optionnally, reagents necessary for performing a hybridization reaction.
In conclusion, it may be underlined that the alliance of such BAC-based approaches such as described in the present specification to the advances in comparative genomics by the availability of an increased number of complete genomes, and the rapid increase of well-characterized gene products in the public databases, will allow the one skilled in the art an exhaustive analysis of the mycobacterial genome.
Materials and Methods
1. DNA-preparation. Preparation of M. tuberculosis H37Rv DNA in agarose plugs was conducted as previously described (Canard et al., 1989; Philipp et al., 1996b). Plugs were stored in 0.2 M EDTA at 4° C. and washed 3 times in 0.1% Triton X-100 buffer prior to use.
2. BAC vector preparation. pBeloBAC11 was kindly provided by Dr. Shizuya, Department of Biology, California Institute of Technology (Pasadena, Calif.). The preparation followed the description of Woo et al., 1994 (Woo et al., 1994).
3. Partial digestion with HindIII. Partial digestion was carried out on plugs, each containing approximately 10 μg of high molecular weight DNA, after three one hour equilibration steps in 50 ml of HindIII 1× digestion buffer (Boehringer Mannheim, Mannheim, Germany) plus 0.1% Triton X-100. The buffer was then removed and replaced by 1 ml/plug of ice-cold HindIII enzyme buffer containing 20 units of HindIII (Boehringer). After two hours incubation on ice, the plugs were transferred to a 37° C. water bath for 30 minutes. Digestions were stopped by adding 500 μl of 50 mM EDTA (pH 8.0).
4. Size selection. The partially digested DNA was subjected to contour-clamped homogenous electric field (CHEF) electrophoresis on a 1% agarose gel using a BioRad DR III apparatus (BioRad, Hercules, Calif.) in 1×TAE buffer at 13° C., with a ramp from 3 to 15 seconds at 6 V/cm for 16 hours. Agarose slices from 25 to 75 kb, 75 to 120 kb and 120 to 180 kb were excised from the gel and stored in TE at 4° C.
5. Ligation and transformation. Agarose-slices containing fractions from 25 to 75 kb, 75 to 120 kb and 120 to 180 kb were melted at 65° C. for 10 minutes and digested with Gelase (Epicentre Technologies, Madison, Wis.), using 1 unit per 100 μl gel-slice. 25-100 ng of the size-selected DNA was then ligated to 10 ng of HindIII digested, dephosphorylated pBeloBAC11 in a 1:10 molar ratio using 10 units of T4 DNA ligase (New England Biolabs, Beverly, Mass.) at 16° C. for 20 hours. Ligation mixtures were heated at 65° C. for 15 minutes, then drop-dialysed against TE using Millipore VS 0.025 mM membranes (Millipore, Bedford, Mass.). Fresh electrocompetent E. coli DH10B cells (Sheng et al., 1995) were harvested from 200 ml of a mid-log (OD550=0.5) culture grown in SOB medium. Cells were washed three times in ice-cold water, and finally resuspended in ice-cold water to a cell density of 1011 cells/ml (OD550=150). 1 μl of the ligation-mix was used for electroporation of 30 μl of electrocompetent DH10B E. coli using a Eurogentec Easyject Plus electroporator (Eurogentec, Seraing, Belgium), with settings of 2.5 kV, 25 μF, and 99½, in 2 mm wide electroporation cuvettes. After electroporation, cells were resuspended in 600 μl of SOC medium, allowed to recover for 45 minutes at 37° C. with gentle shaking, and then plated on LB agar containing 12.5 μg/ml chloramphenicol (CM), 50 μg/ml X-gal, and 25 μg/ml IPTG. The plates were incubated overnight and recombinants (white colonies) were picked manually to 96 well plates. Each clone was inoculated 3 times (2×200 μl and 1×100 μl of 2YT/12.5 μg/ml CM per clone) and incubated overnight. One of the microtiter plates, containing 100 μl culture per well, was maintained as a master plate at −80° C. after 100 ml of 80% glycerol were added to each well while minipreps (Sambrook et al., 1989) were prepared from the remaining two plates to check for the presence of inserts. Clones containing inserts were then designated “Rv” clones, repicked from the master plate to a second set of plates for storage of the library at −80° C.
6. Preparation of DNA for sizing, direct sequencing and comparative genomics. A modified Birnboim and Doly protocol (Birnboim et al., 1979) was used for extraction of plasmid DNA for sequencing purposes. Each Rv clone was inoculated into a 50 ml Falcon polypropylene tube containing 40 ml of 2YT medium with 12.5 μg/ml of CM and grown overnight at 37° C. with shaking. Cells were harvested by centrifugation and stored at −20° C. The frozen pellet was resuspended in 4 ml of Solution A (50 mM glucose, 10 mM EDTA, 25 mM Tris, pH 8.0) and 4 ml of freshly prepared solution B (0.2 M NaOH, 0.2% SDS) was then added. The solution was gently mixed and kept at room temperature for 5 minutes before adding 4 ml of ice-cold solution C (3M Sodium Acetate, pH 4.7). Tubes were kept on ice for 15 min, and centrifuged at 10,000 rpm for 15 min. After isopropanol precipitation, the DNA pellet was dissolved in 600 μl RNase solution (15 mM Tris HCl pH 8.0, 10 μg/ml RNase A). After 30 minutes at 37° C. the DNA solution was extracted with chloroform:isoamylalcohol (24:1) and precipitated from the aqueous phase using isopropanol. The DNA pellet was then rinsed with 70% ethanol air-dried and dissolved in 30 μl distilled water. In general, DNA prepared by this method was clean and concentrated enough to give good quality results by automatic sequencing (at least 300 bp of sequence). For a few DNA preparations, an additional polyethylene glycol (PEG) precipitation step was necessary, which was performed as follows. The 30 μl of DNA solution were diluted to 64 μl, mixed gently and precipitated using 16 μl 4M NaCl and 80 μl of 13% PEG 8000. After 30 min on ice the tubes were centrifuged at 4° C., the pellet carefully rinsed with 70% ethanol, air-dried and diluted in 20 μl of distilled water.
7. Sizing of inserts. Insert sizes were determined by pulsed-field gel electrophoresis (PFGE) after cleavage with DraI (Promega). 100-200 ng of DNA was DraI-cleaved in 20 μl total reaction volume, following the manufacturer's recommendations, then loaded onto a 1% agarose gel and migrated using a pulse of 4 s for 15 h at 6.25 V/cm at 10° C. on an LKB-Pharmacia CHEF apparatus. Mid-range and low-range PFGE markers (New England Biolabs) were used as size standards. Insert sizes were estimated after ethidium bromide staining of gels.
8. Direct sequencing. For each sequencing reaction 7 μl BAC DNA (300-500 ng), 2 μl primer (2 μM), 8 μl reaction mix of the Taq DyeDeoxy Terminator cycle sequencing kit (Applied Biosystems) and 3 μl distilled water were used. After 26 cycles (96° C. for 30 sec; 56° C. for 15 sec; 60° C. for 4 min) in a thermocycler (MJ-research Inc., Watertown, Mass.) DNA was precipitated using 70 μl of 70% ethanol/0.5 mM MgCl2, centrifuged, rinsed with 70% ethanol, dried and dissolved in 2 μl of formamide/EDTA buffer. SP6 and T7 samples of 32 BAC clones were loaded onto 64 lane, 6% polyacrylamide gels and electrophoresis was performed on a Model 373A automatic DNA sequencer (Applied Biosystems) for 12 to 16 hours. The sequences of oligonucleotides used as primers are shown in Table 1.
9. DOP-PCR. As an alternate procedure we used partially degenerate oligonucleotides in combination with vector-specific (SP6 or T7) primers to amplify insert ends of BAC clones, following a previously published protocol for P1 clones (Liu et al., 1995). The degenerate primers Deg2, Deg3, Deg4, Deg6 (Table 1) gave the best results for selected amplification of insert termini. TABLE 1
Primers used for PCRs and sequencing
Vector specific Primers for DOP PCR- first
amplification step:
SP6-BAC1: AGT TAG CTC ACT CAT TAG GCA
T7-BAC1: GGA TGT GCT GCA AGG CGA TTA
Vector specific Primers (direct sequencing,
nested primer for second PCR step)
SP6 Mid: AAA CAG CTA TGA CCA TGA TTA CGC CAA
T7-Belo2: TCC TCT AGA GTC GAC CTG CAG GCA
Degenerate Primers:
Deg2: TCT AGA NNN NNN TCC GGC
Deg3: TCT AGA NNN NNN GGG CCC
Deg4: CGT TTA AAN NNN NWA GGC CG
Deg6: GGT ACT AGT NNN NNW TCC GGC
Primers used for the amplification of M. bovis
DNA in polymorphic chromosomal region of Rv58:
Primer 1: ACG ACC TCA TAT TCC GAA TCC C
Primer 2: GCA TCT GTT GAG TAC GCA CTT CC
10. Screening by pooled PCR. To identify particular clones in the library which could not be detected by random end-sequencing of the 400 BAC clones, PCR-screening of DNA pools was performed. Primers were designed for regions of the chromosome where no BAC coverage was appearent using cosmid-or H37Rv whole genome shotgun sequences. Primers were designed to amplify approximately 400-500 bp. Ninety-six-well plates containing 200 μl 2YT/12.5 μg/ml CM per well were inoculated with 5 μl of −80° C. glycerol stock cultures each from the master plates and incubated overnight. The 96 clones of each plate were pooled by taking 20 μl of culture from each well and this procedure was repeated for 31 plates. Pooled cultures were centrifuged, the pellets were resuspended in sterile water, boiled for 5 minutes, centrifuged and the supernatants kept for PCRs. As an initial screening step, the 31 pools of a total of 2976 BACs, representing about two thirds of the library were tested for the presence of a specific clone using appropriate PCR primers. PCR was performed using 10 μl of supernatant, 5 μl of assay buffer (100 mM b-mercaptoethanol, 600 mM Tris HCl (pH 8.8), 20 mM MgCl2, 170 mM (NH4)2SO4), 5 μl of Dimethylsulfoxide (DMSO), 5 μl of dNTPs (20 mM), 5 μl of water, 10 μl primer (2 μM), 10 μl inverse primer (2 μM) and 0.2 units of Taq DNA polymerase (Boehringer). 32 cycles of PCR (95° C. for 30 s, 55° C. for 1 min 30 s, 72° C. for 2 min) were performed after an initial denaturation at 95° C. for 1 min. An extension step at 72° C. for 5 min finished the PCR-If a pool of 96 clones yielded an appropriate PCR product (FIG. 1A), subpools were made to identify the specific clone. Subpools representative for lane A of a 96 well plate were made by pooling clones 1 to 12 from lane A into a separate tube. Subpools for lanes B to H were made in the same way. In addition, subpools of each of the 12 rows (containing 8 clones each) were made, so that for one 96 well plate, 20 subpools were obtained. PCR with these 20 subpools identified the specific clone (FIG. 1B, lower gel portion). If more than one specific clone was present among the 96 clones of one plate (FIG. 1B, upper gel portion), additional PCR reactions had to be performed with the possible candidates (data not shown).
11. Genomic comparisons. DNA from the BAC clone Rv58 was digested with the restriction endonucleases EcoRI and PvuII, and resolved by agarose gel electrophoresis at low voltage overnight (1.5 V/cm). DNA was transferred via the method of Southern to nitrocellulose membranes (Hybond C extra, Amersham) following standard protocols (Sambrook et al., 1989), then fixed to the membranes at 80° C. for 2 hours. The blot was hybridized with 32P labelled total genomic DNA from M. tuberculosis H37Rv, M. bovis type strain (ATCC 19210) or M. bovis BCG Pasteur. Hybridization was performed at 37° C. overnight in 50% formamide hybridization buffer as previously described (Philipp et al., 1996b). Results were interpreted from the autoradiograms.
12. Computer analysis. Sequence data from the automated sequencer ABI373A were transferred as binary data to a Digital Alpha 200 station or Sun SparcII station and analysed using TED, a sequence analysis program from the Staden software package (Dear et al., 1991). Proof-read sequences were compared using the BLAST programs (Altschul et al., 1990) to the M. tuberculosis H37Rv sequence databases of the Sanger Centre, containing the collected cosmid sequences (TB.dbs) and whole-genome shotgun reads (TB_shotgun_all.dbs) (http://www.sanger.ac.uk/). In addition, local databases containing 1520 cosmid endsequences and the accumulating BAC endsequences were used to determine the exact location of end-sequenced BACs on the physical and genetic map. MycDB (Bergh et al., 1994) and public databases (EMBL, Genbank) were also used to compare new sequences, but to a lesser extent. The organization of the open reading frames (ORFs) in the polymorphic region of clone Rv58 was determined using the DIANA software established at the Sanger Centre.
EXAMPLES Example 1 Construction of a pBeloBAC11 Library of M. tuberculosis H37Rv Partial HindIII fragments of H37Rv DNA in the size range of 25 to 180 kb were ligated into pBeloBAC11 and electroporated into strain E. coli DH10B. While cloning of fractions I (25 to 75 kb) and 11 (75 to 120 kb)-gave approximately 4×104 transformants (white colonies), cloning of fraction m (120 to 180 kb) repeatedly resulted in empty clones. Parallel cloning experiments using partial HindIII digests of human DNA resulted in stable inserts for all three fractions (data not shown), suggesting that the maximum size of large inserts in BAC clones is strongly dependent on the source of the DNA. Analysis of the clones for the presence of inserts revealed that 70% of the clones had an insert of the appropriate size while the remaining 30% of white colonies represented empty or lacZ′-mutated clones. Size determination of randomly selected, DraI-cleaved BACs via PFGE showed that the insert sizes ranged for the majority of the clones between 40 kb and 100 kb with an average size of 70 kb. Clones with inserts of appropriate size were designated with “Rv” numbers, recultured and stored at −80° C. for further use.
Example 2 Direct DNA Sequence Analysis of BACs To characterize the BAC clones, they were systematically subjected to insert termini sequencing. Two approaches, direct sequencing of BAC DNA and PCR with degenerate oligonucleotide primers (DOP), adapted to the high G+C content of mycobacterial DNA, were used. In a first screening phase, 50 BAC clones designated Rv1 to Rv50 were analysed using both methods in parallel. Except for two clones, where the sequences diverged significantly, the sequences obtained by the two methods only differed in length. Sequences obtained directly were on average about 350 bp long and for 95% of the clones both the SP6 and T7 endsequences were obtained at the first attempt. Sequences obtained by DOP-PCR were mostly shorter than 300 bp. For 40% of the BACs we obtained only very short amplicons of 50 to 100 base pairs from one end. In two cases the sequence obtained with the DOP-PCR differed from the sequences obtained by direct sequencing, and in these cases E. coli or vector sequences were amplified (data not shown). Taking the advantages and disadvantages of both methods into account, we decided to use direct termini sequencing for the systematic determination of the SP6 and T7 end-sequences.
Example 3 Representativity of the Library After having determined the end-sequences of 400 BACs a certain redundancy was seen; The majority of clones were represented at least 3 to 4 times. Maximum redundancy was seen in the vicinity of the unique rrn operon, as 2.5% of the clones carried identical fragments that bridge the cosmids Y50 and Y130 (FIG. 3, approximate position at 1440 kb). The majority of clones with identical inserts appeared as two variants, corresponding to both possible orientations of the HindIII fragment in pBeloBAC11. This suggests that the redundancy was not the result of amplification during library construction, but due to the limited number of possible combinations of partial HindIII fragments in the given size-range of 25 to 120 kb. To detect rare BAC clones, a pooled PCR protocol was used. Primers were designed on the basis of the existing cosmid sequences and used to screen 31 pools of 96 BAC clones. When positive PCR products of the correct size were obtained, smaller subpools (of 8 or 12 clones each) of the corresponding pool were subsequently used to identify the corresponding clone (FIG. 1). With this approach 20 additional BACs (Rv401-Rv420) were found for the regions where no BACs were found with the initial systematic sequencing approach. The endsequences of these BACs (Rv401-420) were determined by direct sequencing, which confirmed the predicted location of the clones on the chromosome. A 97% coverage of the genome of H37Rv with BAC clones was obtained. Only one region of ˜150 kb was apparently not represented in the BAC library as screening of all pools with several sets of specific primers did not reveal the corresponding clone. This was probably due to the fact that HindIII fragments of mycobacterial DNA larger than 110 kb are very difficult to establish in E. coli and that a HindIII fragment of ˜120 kb is present in this region of the chromosome (data not shown).
Example 4 Establishing a BAC Map Using all endsequence and shotgun-sequence data from the H37Rv genome sequencing project, most of the BAC clones could then be localized by sequence comparison on the integrated map of the chromosome of M. tuberculosis strain H37Rv (Philipp et al., 1996b) and an ordered physical map of the BAC-clones was established. PCR with primers from the termini sequences of selected BACs were used for chromosomal walking and confirmation of overlapping BACs (data not shown). The correct order of BACs on the map was also confirmed more recently, using 40,000 whole genome shotgun reads established at the Sanger Centre. In addition, pulsed-field gel electrophoresis of DraI digests of selected BACs was performed (FIG. 2) in order to see if the approximate fragment size and the presence or absence of DraI cleavage sites in the insert were consistent with the location of the BACs on the physical map (FIG. 3). Comparison of the sequence-based BAC-map with the physical and genetic map, established by PFGE and hybridization experiments (Philipp et al., 1996b), showed that the two maps were in good agreement. The positions of 8 genetic markers previously shown on the physical and genetic map were directly confirmed by BAC-endsequence data (Table 2, FIG. 3). The position of 43 from 47 Y-clones (91%) shown on the physical and genetic map, which were later shotgun sequenced, was confirmed by the BAC endsequences and shotgun sequence data. Four clones (Y63, Y180, Y251, and Y253) were located to different positions than previously thought and this was found to be due to book keeping errors or to chimeric inserts. Their present approximate location relative to the oriC is shown in FIG. 3: Y63 at 380 kb, Y63A at 2300 kb, Y180 at 2160 kb, Y251 at 100 kb, and Y253 at 2700 kb. A total of 48 BACs, covering regions of the chromosome, not represented by cosmids were then shotgun sequenced (Cole et al., 1997), and these are squared in FIG. 3. No chimeric BACs were found, which is consistent with the observations of other research groups for other BAC libraries (Cai et al., 1995; Zimmer et al; 1997). The absence of chimeric BACs was of particular importance for the correct assembly of the M. tuberculosis H37Rv sequence. The exact position of the BAC termini sequences on the chromosome will be available via the world wide web http://www.pasteur.fr/MycDB). TABLE 2
Identities of genetic markers previously shown on the integrated
and genetic map of H37Rv (Phlipp et al., 1996b) wich showed
perfect sequence homology with BAC ens. sequences.
BAC GenBank
end Description of Accession
Locus sequence genetic marker Organism n°
apa Rv163SP6 Secreted M. tuberculosis X80268
alanine-proline-
rich antigen
dnaJ, dnaK Rv164T7 DnaJ hsp M. leprae M95576
fop-A Rv136T7 Fibronectin M. tuberculosis M27616
binding protein
pa/A Rv401T7 DNA polymerase M. tuberculosis L11920
I
ponA Rv273T7 Penicillin binding M. leprae S82044
protein
pstC Rv103T7 Putative M. tuberculosis Z48057
phosphate
transport receptor
recA Rv415SP6 Homologous M. tuberculosis X58485
recombination
wag9 Rv35SP6 35-kDa antigen M. tuberculosis M69187
Example 5 Repetitive Endsequences Repetitive sequences can seriously confound mapping and sequence assembly. In the case of the BAC endsequences, no particular problems with repetitive sequences were observed. Although nine clones with one end in an IS1081 (Collins et al., 1991) sequence were identified, it was possible to correctly locate their position on the map using the sequence of the second terminus. Moreover, these BACs were used to determine the exact locations of IS1081 sequences on the map. Five copies of this insertion sequence, which harbors a HindIII cleavage site, were mapped on the previous physical and genetic map. In contrast, BAC endsequence data revealed an additional copy of IS1081 on the M. tuberculosis H37Rv chromosome. The additional copy was identified by six clones (Rv27, Rv118, Rv142, Rv160, Rv190, Rv371) which harbored an identical fragment linking Y50 to 1364 (FIG. 3, at ˜1380 kb). This copy of IS1081 was not found by previous hybridization experiments probably because it is located near another copy of IS1081, localized on the same DraI fragment Z7 and AsnI fragment U (FIG. 3, at ˜1140 kb). Furthermore, the position of a copy of IS1081 previously shown in DraI fragment Y1 (FIG. 3, at ˜1840 kb) had to be changed to the region of Y349 (FIG. 3, at ˜3340 kb) according to the endsequences of BAC Rv223. The positions of the four other IS1081 copies were confirmed by the sequence data and therefore remained unchanged. In total 6 copies of IS1081 were identified in the H37Rv genome in agreement with the findings of others (Collins et al., 1991).
In addition, a sequence of 1165 bp in length containing a HindIII site was found in two copies in the genome of H37Rv in different regions. The endsequences of BAC clones Rv48 and Rv374, covering cosmid Y164, as well as Rv419 and Rv45, that cover cosmid Y92, had perfect identity with the corresponding parts of this 1165 bp sequence (FIG. 3, at ˜3480 kb and ˜900 kb). Analysis of the sequence did not reveal any homology with insertion sequences or other repetitive elements. However, as each of the two locations showed appropriate BAC coverage, chimerism of the sequenced cosmids Y164 and Y92 can be ruled out as the probable cause.
Example 6 Using BAC Clones in Comparative Genomics The minimal overlapping set of BAC clones represents a powerful tool for comparative genomics. For example, with each BAC clone containing on average an insert of 70 kb, it should be possible to cover a 1 Mb section of the chromosome with 15 BAC clones. Restriction digests of overlapping clones can then be blotted to membranes, and probed with radiolabelled total genomic DNA from, for example, M. bovis BCG Pasteur. Restriction fragments that fail to hybridize with the M. bovis BCG Pasteur DNA must be absent from its genome, hence identifying polymorphic regions between M. bovis BCG Pasteur and M. tuberculosis H37Rv. The results of such an analysis with clone Rv58 (FIG. 3, at −1680 kb) are shown here. This clone covers a previously described polymorphic genomic region between M. tuberculosis and M. bovis BCG strains (Philipp et al., 1996a). EcoRI and PvuII digests from clone Rv58, fixed on nitrocellulose membranes, were hybridized with 32P-labelled total genomic DNA from M. tuberculosis H37Rv, M. bovis (ATCC 19120), and M. bovis BCG Pasteur. FIG. 4 presents the results of this analysis, where it is clear that several restriction fragments from clone Rv58 failed to hybridize with genomic DNA from either M. bovis or M. bovis BCG Pasteur. On the basis of the various missing restriction fragments, a restriction map of the polymorphic region was established and compared to the H37Rv sequence data. The localization of the polymorphism could therefore be estimated, and appropriate oligonucleotide primers (Table 1) were selected for the amplification and sequencing of the corresponding region in M. bovis. The alignment of M. bovis and M. tuberculosis H37Rv sequences showed that 12,732 bp were absent from the chromosomal region of the M. bovis type strain and M. bovis BCG Pasteur strain. The G+C content of the polymorphic region is 62.3 mol %, which is the same as the average genome G+C content of the M. tuberculosis genome, hence indicating that this region is not a prophage or other such insertion. Subsequent PCR studies revealed that this segment was also absent from the Danish, Russian, and Glaxo substrains of M. bovis BCG, suggesting that this polymorphism can be used to distinguish M. bovis from M. tuberculosis. Analysis of this sequence showed that 11 putative open reading frames (ORFs) are present in M. tuberculosis, corresponding to ORFs MTCY277.28 to MTCY277.38/accession number Z79701-EMBL Nucleotide Sequence Data Library (FIG. 5). FASTA searches against the protein and nucleic acid databases revealed that the genes of this region may be involved in polysaccharide biosynthesis. Among these putative genes, the highest score was seen with ORF 6 (MTCY277.33), whose putative product shows a 51.9% identity with GDP-D-Mannose dehydratase from Pseudomonas aeruginosa (accession number U18320—EMBL Nucleotide Sequence Data Library) in a 320 amino acid overlap. The novel M. bovis sequence of the polymorphic region was deposited under accession number AJ003103 in the EMBL Nucleotide Sequence Data Library.
As it appears from the teachings of the specification, the invention is not limited in scope to one or several of the above detailed embodiments; the present invention also embraces all the alternatives that can be performed by one skilled in the same technical field, without deviating from the subject or from the scope of the instant invention. TABLE 3
End sequences of the polynucleotide inserts cloned in the named recombinant
BAC vectors contained in the I-1945 M. tuberculosis H37Rv genomic DNA library.
RvXXXSP6 corresponds to the SP6 endsequence of the clone RvXXX;
RvXXXT7 corresponds to the T7 endsequence of the clone RvXXX.
RvXXXIS 1081 corresponds to a region located close to a copy of the IS1081
repetitive sequence (Insertion element)
The character << - >> denotes an uncertain base residue.
Clone Rv101
:::::::::::::::Rv101SP6.seq::::::::::::::
AATACTCAAGCTTGCCCAGCCGTCGATGACAAGAAATATGTCCGCAAAAGACTCAGCGGCCGACTTTGCTCGCAGCTGGCGGTACCGCGCCACCGATTCTATGCCG
TGGTCGCGGAAAAATGCCTCCCGAAATCGCACGGCCGACTCCAGTTCGGCGAGCATCCGCGATGCCAGCTGCGGCTGCGCCCTGCCGGCCACGGCACCCTCATGCG
GCAGTTCGTCCACCTGGGCCAGCGCCCCGCCGCCGAATTCCAAACAATAGAACTGCACCCGGCCCGCATCGTGGGTAACAGCCAACGGCATGATCAGCGTCGGCAG
CGCGGTTGACTTGCCCGTTTGCGGTGCACCTACGAACGCGACATTGCCTGCGGCCCCGGACAAGTCGATCGTGCGCGGCACCCGTGACTGCTCTAACGGGCGATTG
AAATTCCGAT
:::::::::::::::Rv101T7.seq:::::::::::::::
CCACCCGTGTAATTTGGGATGGGCAAAAAGGCGAAGCACCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGCGGTTAGGGCTTCTCGCGGGAAGGCCCGAAC
GTACGGCGTTTCAACACCTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGCTGGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGG
TAGTCGCCGCCCGGGCGGCTACACTCTGAAACGCGATGACCATCGATGTGTGGATGCAGCATCCCGACGCAACGGTTCCTACACCGCGATATGTTCGGCTCGCTGC
CCCGGTGGACCGGT
Clone Rv102
:::::::::::::::Rv102SP6.seq:::::::::::::::
AATACTCAAGCTTTCCGCCGATACCCGCCATGTCGCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGGATCCCAAAGTGCGGATGATCGGGCCGCC
TACGTCGTGGTGTACCTCGTCGGTAACAACGAAACCGAAGCGTATGACTCGGTCCACGCGGTGCGGCACATGGTGGACACCACACCGCCACCGCACGGGGTGAAGG
CCTATGTCACCGGTCCGGCAGCACTCAATGCCGACCAGGCCGAGGGCGGAGACAAAAGTATCGCTAAGGTCACCGCGATCACCAACATGGTGATCGCAGCAATGTT
GCTAGTGATCTATCGCTCCGTAATTACCGCGGTTCT
:::::::::::::::Rv102T7.seq:::::::::::::::
GTGCCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACACCTCGATGCTGCCGCCATGG
ACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTGGA
TGACGGCATCGCCACCGGAGCGACGGCCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCAGACGACATC
GTGGCGAGATTCGCCGGGTACGCCGATGAGGTGGT
Clone Rv103
:::::::::::::::Rv103SP6.seq:::::::::::::::
AATACTCAAGCTTTCGGCGGAAACGGACACACATTGAATATTGATGACAAAATAAAAATCATTGATGGTTTGAGTCACCAGGCCGATCAAGCCTTCGCCGAGCCAA
ATTCCAATCAAGAGGCCCAAGCCCGTACCAATCAGCCCGGCAACGAGGGATTCCGTCATTATCAGCCAAAATAACTGCTCTCGGGTTACACCCAAACAGCGCAATA
TGGCGAAAAACGGTCGCCGTTGCACGACATTAAATGTCACGGTATTGTAGATTAAAAAGATACCCAC
:::::::::::::::Rv103T7.seq:::::::::::::::
TGCTCCCGAAACCTGGGGGTGTGCCTGCTCTGTATGCACGGCATACGGACATCCTTCCCCTGAGACCCGCGGTCGAACCAGCCACGTGTCCATCATAGNGGGTCAA
CCCCGGCCAAGGGCGACGGCACGCCAAGTTCGCCGACCGTTAACCTAGTGCTGTTAGCTTCATTTGCTGCGATCAAAACAGCTGGTCGGCCGTTAGGAACTGAATT
GAAACTCAACCGATTTGGTGCCGCCGTAGGTGTCCTGGCTGCGGGTGCGCTGGTGTTGTCGGCGTGTGGTAACGACGACAATGTGACCGGGGGAGGTGCAACCACT
GGCCAGGCGTCGGCAAAGGTCGATTGCGGGGGGAAGAAGACACTCAAAGCCAGTGGGT
Clone Rv104
:::::::::::::::Rv104SP6.seq:::::::::::::::
ATACTCAAGCTTTGCCGACGAGCGGGCGATGTTGATGACGGGAAACCCCAGCGCACAACCGACGATTTTGGCGTAGCCGGCGGACGTCTGCTCGATTCCGATCACG
TCGGCGCTCGCATCGAGCATGGCGCCGGCGACGGCTAGCAGCGATCCGCCGTCGTCGAGAAGCACGACACGAGCCGTACGCCCGGCCGTAAGCCGCGCCCAGGATT
CGGCGAAAAACCGTTCTACGTGGCGGGTGTACTGGGTGTCGAATGATTCGTGGGGTGCGTAGGCGTCGCTGCAATCGTCGACATAGATGCCGTCGGGCCGCATCGC
GTCGACAACTCCGGGTGAGTGCAATAGCACTTGCCGATCACCGCGACGTTGCGCGGATGAGGCCGAACCCGAATA
:::::::::::::::Rv104T7.seq:::::::::::::::
TCCTATGTCCCTGCCGAGCANGTGATCGAACGCGGTGACAGATTTGTCTATCCTGGACCTGACGGTGAGGTCGAAGTTTTCCAGGAATTCGGCAAAATCGGTAAGA
GCCTGAAGAATTCGGTATCGCCGGACGAAATCTGCGACGCATACGGGGGCATATACGCTTCGGGTTTACGAGATGTCGATGGGGCCGCTGGAGGCTTCACGTCCAT
GGGCCACAAAGGATGTTGTCGGCGCGTACCGTTTTCTGCAGCGGGTGTGGCGCTTGGTCG
Clone Rv105
:::::::::::::::Rv105SP6.seq:::::::::::::::
ATACTCAAGCTTGATTCCGCCGAAACCGACCGTGAGCACCCCGCCAGCCACCACGCTCGGGTCGGGCGCCGGGCCCGGGCCGCCAGGCTGCTCCGCTCGGTGATGG
CACGCCACCGCGACACCACCCGGCTGCGCTACGTCTAACCATTCCAGGCGGAGCTACATCAGCTCGGCCGCCCAGTGTTCGGGGCCTCTTTCCAGGTCGAAGTCTA
TACCGATATGCGCATCCGCAGCCGCCACCCTGGAGAACAGAACGATGCCCTACTAATGCTTGTCTGGCGGGGCC
:::::::::::::::Rv105T7.seq:::::::::::::::
GGTACGCTTCGGTCGCAGTCTGCGAGTGATGCATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCACACCTTCAGTTGCTCACCGGAATCCAACCGGTA
GAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTCGGGGTGGTCGCGA
CGCGCATGGGCCACCATCGCATTCACCAGGTCTGCGCGAATCACCAGCACGTAGACGGTTCCTTTCCTAAGCAACACCGAAGTTTCAGGACCCGAATGCTCCGGGA
AACATGTCACGGTAGGTCGGTATTCCGGCTACCGGCTGA
Clone Rv106
:::::::::::::::Rv106SP6.seq:::::::::::::::
GGCGTCAACGGTGTCGGAACCCGCGTCAAGCAATTGGTAGGCCTGCAGTCTGTGAATCAGGCCGACGCTGTGGCCGCCGCGGC
:::::::::::::::Rv106T7.seq:::::::::::::::
GGCTNGCGTACCCGGTACCGGCCGCGGGCCTACCACGTGCCGGAACTGGAAGCGCAGTAAGCCCTCAACGCGCCACCGCTTTGGCCCGGGGGCCCGGCGTAGGCGC
ATCGGCGGTGGCCGTGGGGCGGCGCACTGCGACCTCACCAGCGGCTTTCGAGCTTTGTTCGATCAACCGGCCAGCATGGTCGANGATGCATTCGAGACCATATTCG
AAATTGGTTTCATCGGGGGCCCCGATCCGATGCCCCCTCCCAGTTGCGTGAGCAANCAGCGGAGTCNTCGCGGGATCGATGGCCACGGGGTGTTCAATGGCGGATG
GTCCGCTGCCCGCCGACTGGCTCTTGCGGGAGAACCGATCTAGCACCACCGATCCGCGCACGTNG
Clone Rv107
:::::::::::::::Rv107T7D4.seq:::::::::::::::
CGTAATNTCGCGCACANCCANGACTTCTGGGGGGATCNGCTGACAGTGGTNGGATCCCAAATTGCGGATGATCGGGCCGCCNACGTCGTTGTGTACCTCNTCNGTC
ACAACNAANCCGAANCGTATGACTCGGTCCACGCGGTGCGGCACATGGTGGACACCACACCGCCACCGCNCGGGGTGAAGGCCTATGTCACCGGTCCGGCAACACT
CAATGCCGACCAGGCCGANGCCGGACACNANAGTATCNCTAACGTCACCGCGATCACGAGCATGGTGATCGNNCAATGTTNCTANTGATCTATCGCTCCGTAATTA
CCGCGGTTCTCGTCTTGATCATGGTCGCANCGAACTCCGGCGCAATCCGCGGATTCATCGNCTTGCTCGCCGATCACATATTTTCAGCCTTTCACATTGCAACNAA
CCTGCTCGTCTCATGGNGATGCGGCGACACGGACTACCGATATCATGCTCGCCGTTACACAATCNCGCCACGCCGCGAAGACNGGAAACGCTTCTACACAATNTTC
NCGGGACGCCACTNAACTTGGTTCNGGTTTGACATTGCCGCGCATGTNTGCCCAGCTTTGCCGGCTCCCCTTA
Clone Rv108
:::::::::::::::Rv108T7D4.seq:::::::::::::::
TGAATTTCCCGATCCCACAATCTCGGTTCAGATACAGGTCGCCATACCCCTTACTTCGGCAACGCTGGGCGGATTGGGGCTGCNGCTGCAGCANACCATCGACGCC
ATCGAATTGCCGGCAATCTCGTTCAGCCAATCCATACCCATCGACATTCCGCCGATCGACATCCCGGCCTTCNCCCTTTAACGG
Clone Rv109
:::::::::::::::Rv109SP6.seq:::::::::::::::
AACAGCTATGACCATGNTTACGCCAAGCTATTTAGGTAACACTATANAATACTCAAGCTTTTACGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGCAGCGCA
NCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCCGCGGTCNGGTGCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACCCTGAAACCAGCTTCC
ATATCCCGCGACNAACNACNCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAGCGCGTTCTCCACCGACCGGGCCCGGGTGTGGGGTGTTTGGGC
GACCGGCAGCCAGGTGGTCCACACTGCCGACGGGCGCCGCGAGCCGTTCACCGACCAAGCCGCCGAACAAGTCCGCCCGATCGCATACTCCAACCGGTTGCGGTAC
TGCAGGTCAGCTGGCGTACCTCCTCNTCNCGCTCGGCGAAGTCTTGCTCCANCACGTCGCAGAACGGCAAGGAACACGTTCA
:::::::::::::::Rv109T7seq:::::::::::::::
GACCGNNCCATGTTTCCACAATGTGGTGCCAGTNCGGNGGCTACGTGCCATCNANACACTGGCGCAGGCTATCGCACCCGTTATCNGCTACGAACAAATCNCGGTA
TGCGTTCTTTANCATGAGTCGGCGACCGNCGATCATGGTCGACACCCACGACNGAAATACGCAGATCGCCNTCNAGCNTGTGTGCCGCGGATTATCANGACTGACC
TCCTGGCTGACCGGNNTGTNTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGNTCGTGGTCGGCTCGGATAGCGAAGTCAGCTAATTCTCGTGGCAGCTCGAAAGGG
TCCTGCCGGTGCCGGTCTTTGCGCAAACCATGCNCATGTTACGGTCCCTCGGGTGCGGCCTGGCGGCGGC
Clone Rv10
:::::::::::::::Rv10SP602.seq:::::::::::::::
GGGATGGGCGGGCCCGCTAAACTCTTCGTGTTCCACTAACTCCGGGAGGGNCAATCTCGGGCCGTTATGGCTCACGTCGCGTCGCCCTCCGACCGCGAACATTCGG
AGTTGGCAGCAACCTGGTAGCACCCTGGCCGG
:::::::::::::::Rv107TD4.seq:::::::::::::::
NCCGTCGTTGACAAGTAAATATGTCCGCAAAAGTCTCAGCGGCCGACTTTGCTCGCAGGTGGCGGTACCGCGCCACCGAGTCGATGCCGTGGTCGCGGAAGAATGC
CTCCCGAAATCGCACGGCCTTCCCNNTTTAAACGGA
Clone Rv110
:::::::::::::::Rv110SP6.seq:::::::::::::::
TTTAGGTGACACTATAGAATACTCAAGCTTTTGGTCTAGCCGGCCGAGCACGATACGGGTGTCATTGGCCACCGGCGGCGGCTGTCCGGGAAATGGCGGGTCCCCG
GTGGTTTTGCTGATGAGTGCTGAACCGTANTCGAAGTGGGCGGCGTCAGACTCCACCCANCCAGCAGGCAGCGCGAAGCTGAATCCTCCAACCGGGTTGTCNATCC
GGACAAGTTGGGGTGCGTTTGGGGCAATGACAGGTGGCNGCGGTGCGTTCGGGTCCGCCGGCCGGAAGTGCTGCGTTGGATCNCCCGCTGGGCATTGGGCNTTTTT
GCGGCGGCCGGTGGTNGGGGGGCAACAGGTNTCCCNGTGCGGGTGGCGCTCAACGGTCNACGGCGCAAGCCGCCGTTGTTGGTACCNGGGGCGCTGGCTCCGGATC
GCGTTGGCGGTCNCCGG
:::::::::::::::Rv110T7.seq:::::::::::::::
CTACACCATCGAATACGACGGCGTCGCCNACTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTAC
TTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGANAACCTGCCGCTGC
TAGAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCAACCAAACTTGAANGTGATTGTTAACCTGGGCTACNGCGACCCGGCCTATGG
TTATTCNACCTCNCCGCCCAATGTTGCGACTCCGTTCGGGTTGTTCCCANAAGTCNNCCCGGTCGTCATCGCCGAANCTCTCNTCCCGGGACCCACAGGGAATCNG
CNATTTCNCCTACAAATCANCCACCTCCA
Clone Rv111
:::::::::::::::Rv111T7.seq:::::::::::::::
GCATGATCGGCCACCTTTCGGGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCGCGGGTAATCGCCGACGGTGCCGGTTCGCGAGCCGAAGGT
GACGACTCTGATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCACCAACGGCGCGAGCTCAACGACGTCAATCNCGTTGTCGCTTTCTACGGTCACCGACCCTGGT
GACCGTATGGCNCCCG
Clone Rv112
:::::::::::::::Rv112SP6.seq:::::::::::::::
GACACTATAGAATACTCAAGCTTGCCAACCGCCAGCCTGCATCCGGCGGCGANCACTGCTCCGCCGACCNGTACGAACCAACCTGCGGTGCCCAGGCCATTGACGA
TGTGCTGGTCGGCGCCCGCGAGTCCGCGCACCATCAACGCCGCGGGCACCACCANGGCGGCCCCACCCTGCACGGCGACGATCATTCCGGCGCCGCTCACGGCGGG
CGGGGCTCGAACANGCACAGCATCAACGTNGTCACCCGGCCGTGACCGGCCCGCATCGTCACACCACCCAAGCCCATTGCCGTCCTCCTCAACNGGGCGACCCGGC
CCGCATCGTCACACGGNCTAAGGCCATTGCCGTCCTCCT
:::::::::::::::Rv112T7.seq:::::::::::::::
TCGGCGCCATCGGCACCTTCGAGGACCTGTATTTCGACGCCGTGGCCNACCTGAGGTTGGCGGTGGACNAAGTGTGCACCCGGTTGATTGGCTCGGCCTTGCCGGA
TGCCACCCNGCGCCTGGTGGTCGATCCGCNAANAGACAANTTGTGGTGGANGCTTCTGCTGCCTGCGACACCCACNACGTGGTGGCACCGGGCAGCTTTAGCTGGC
ATGTCCTGACCGCGCTGGCCGACNACTCCAGACNTTCCACNAANGGTCGCCNNCCCAATGTNCCGNANTGTCTCCGGNTCCCTTTACCNCCCAATGGGCNGNTTCC
ACNGGTTACGGGCCCCNTNCCGGCGGGTCTNCCTCCCAANCTACCAAATACGCCCGACNTTCCGGA
Clone Rv113
:::::::::::::::Rv113SP6.seq:::::::::::::::
ATACTCAAGCTTTTATGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCG
GGTGCAGGTGCTCGGGCAGCTCGGCCGCGAACAGCCCGGCTTGAACCCTGAAAACCNGCTTTCCATATCCCGCGACGAAAGAACGCCAGTTCCGCTACTTAAGCCC
TCCGCGAACCGTCCATGGACAACAGCGCGTTCTCCACCAACCGGGCCCGGGTGT
:::::::::::::::Rv113T7.seq:::::::::::::::
TCGGCTCAGGCCGCGCTGCTGGTAGAGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGCGCAGGCTATCG
CACCCGTTATCGGCTACGAAGCAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGCCGTCA
AGCATGTGTGCCGCGGATTATCAGGACTGACCTCCTGGCTGACCGGCATGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCGGCTCGGATAGCGA
GGTCAGCGAATTCTCGTGGCAGCTCGAAAGGGTCCTGCCGGTGCCGGTCTTTGCGCAAACAATAGCGCAGGTTACGGTCGCGCGGGGTGCGGCCTGGCGGCGGCC
Clone Rv114
:::::::::::::::Rv114SP6.seq:::::::::::::::
CAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTCGCGTCTACGCCGGCCCGGAGCATCCGCACAGCGCTCAGCAGCCGGTTCCGTACGANCTCAAGCAGGTG
GCGCAATGACCGAAACCACCCCAGCCCCGCAAACCCCGGCGGCCCCGGCCGGGCCCGCACAATCGTTCGTGTTGGAGCGGCCCATCCANACCGTTGGGCGCCGTAA
GGANGCCGTGGTACGAATGCGGCTGGTGCCCGGCACCGGCAAGTTCGACCTCAACGGCCGCAGCTTGGANGACTACTTCCCAAACAAGGTGCACCAGCAGTTGATC
AAGGCACCCCTGGTCACCGTGGATCGGGTGGAAAGTTTCGACATCTTTGCCCACCTGGGCGGCGGCGGCCGTCCGGTCAGGCCGGGCCTGCCCTGGGTATCGCCCG
GGCATTGATTCTGGTATCCCCNGAAGAACCG
:::::::::::::::Rv114T7.seq:::::::::::::::
CGGTTGGCCACCGCTTCTGCGGTGCCGCCGCCGTCGACAATGACCGTGTCGTCCTTGCTGACCACCACGCGTCGGGCCGAGCCCAGCACCTCCAAGCCCACCTCGC
GCAGCACCATGCCGGCGTCGGGGTTGACCACCTGGCCACCCGTCACCACCGCCAGGTCCTCAAGGAAACGCCTTACGGCGGTCACCGAMGTACGGCCCCTTGACCG
CGACCGCTTTCAACGTCTTGCGAATCGCGTTGACGACCAGCGTCGCCAACGCTTCGCCCTCCACGTCTTCAGCCACGATCAGTAGTGGCTTACCCGTTCCTGCAAC
CTTTTCCAGCAATGGCAACAGATCGGGAAGCGANCTGATCTTGTCTTGGTGCN
Clone Rv115
:::::::::::::::Rv115SP6.seq:::::::::::::::
CCAAGCTATTTAGGTGACACTATAGAATACTCAAGCTTTTGGCTGGGTCGCCTTCGAATTCNGCGTGCACCGCTATGGGTTGCANCAGCGGCTGGCGCCGCACACC
CCACTGGCCCGGGTGTTTTCGCCCCGAACCCGGATCATGGTGAGCGAAAAGGANATTCNCCTGTTCGATGCTGGATTCGCCACGCCAAGGCATCTANACGATTACT
CTCCNCGGGGTGGGAAAAGTGCCCAATCCCCCTCCCTCCAACTTTCCNAACAATCATTCCGGTTCCNCCNTCCGGTTGGNGGTAACCNNCCAATAAAACCCCTGCC
CG
:::::::::::::::Rv115T7.seq:::::::::::::::
GCCCGCNCATGGCCAATCCCCGAAGACATCATTGGCCAGTGGCCGGGCGCTAACAGGTTCCAGCCCCCCACCANTGCCGCTCGAACATGCGGTGCAACCCATTCGC
AGGCCGGCAGGGAAAGCACCGCGGAAGCCGCAAAGGGCTGCAGTTCCGCGCCCAATAATGTCGTCCGCAACCAGATGCGCTCNAAAACCNCNCCGGCAGTCAGCGC
ACCCGACGCGANGTCGAAAGACGTCNTCAGCGCGCCCACATGGGGTGCCAATCGGCACGGCAGGTATGCCGCGCGCAACCCGAGCGCGTGGTGCATGCCCACGGTC
CGCANGANGCGCANCACCCGCCAATGCCGAANCCCACGAAACATCGGGCGCATCCACCTTCAACC
Clone Rv116
:::::::::::::::Rv116SP6.seq:::::::::::::::
ATACTCAAGCTTGCCCAGCCGTCGATGACAAGAAATATGTCCGCAAAAGACTCAGCGGCCGACTTTGCTCGCAGCTGGCGGTACCGCGCCACCGAGTCGATGCCGT
GGTCGCGGAAGAATGCCTCCCGAATTCGCACGGCCAATTCCATTCCGGGAAGCATCCGCAATGCCAGCTGCGGTTGCCCCCTGCCGGCCACGGCACCCACTTGCGG
CATTGCGTCCACCTGGGCCAGCGCCCCGCCGCCAAATTCCAAACAATAAAAATTGCACCCGGC
:::::::::::::::Rv116T7.seq:::::::::::::::
CCACCCGTGTATTTTGGGATGGGCAAAAAGGCGAAGCACCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGAAGGCCCGAAC
GTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGGTAGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGG
TAGTCGCCGCCCGGGCGGCTACAGTCTGAAACGCGATGACCATCGATGTGTGGATGCAGCATCCGACGCAACGGTTCCTACACGGCGATATGTTCGCCTCCCTGCC
CCGT
Clone Rv117
:::::::::::::::Rv117SP602.seq:::::::::::::::
CTGCCCATGTTTGGGGACGCCCGACCAGCCGATGCTGGAGGCCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGAGCGGCTGCAACTGGGCGCGTTGGTGACC
GGCAATACCTACCGCAGCCNGACCCCTNTCNCAANAGGATNTTGTTCGCCGGACCCCNCTC
:::::::::::::::Rv117T7D4.seq:::::::::::::::
CCGACTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGA
CGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATC
GTGGGGAACCCACTGGCGAACCTGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGCGACCGCCTTT
Clone Rv118
:::::::::::::::Rv118SP6.seq:::::::::::::::
ATACTCAAGCTTTGTCACACCAAGTGTTTCGACCAGGCGCTCCATCCGGCGAGTGGATACTCCCAGCAGGTAGCAGGTCGCCACCACGCTGGTCAGTGGGCGTTCA
GCTCGCTTGCGGCGCTGCAGCAGCCATTCGGGGAAATACCTGCCCTGGCGCAGCTGGGGGATCCCAACTTCAATGGTTGCGGCACGGGTGTCAAATTCACGGTGGC
GGTAGCCGTTGCCCTAATTGGACCGCTCATCGCTGCTTTCGCGGTACCCCGCCCCGCACAGGGCTTCGGCTTCAGCCCCCATCAGGGCGGCAATAAACTTCAAGAG
CACC
:::::::::::::::Rv118T7.seq:::::::::::::::
GAGGCAGCTTCGCCGGCAATTCTACTAGCGAGAAGTCTGGCCCGATACGGATCTGACCGAAGTCGCTGCGGTGCAGCCCACCCTCATTGGCGATGGCGCCGACGAT
GGCGCCTGGACCGATCTTGTGCCGCTTGCCGACGGCGACGCGGTAGGTGGTCAAGTCCGGTCTACGCTTGGGCCTTTGCGGACGGTCCCGACGCTGGTCGCGGTTG
CGCCGCGAAAGCGGCGGGTCGGGTGCCATCAGGAATGCCTCACCGCCGCGGCACTGCACGGCCAGTGCCGCGGCGATGTCAGCCATCGGGACATCATGCTCGGGTT
CATACTCCTCGACCAGTCGGCGGAACAGCTCGATTCCCGGACCGCCCAGCGCATTGGTGATGGAATCGGCGAACTTGGCCACCCGCTGGGTGTTGACATCCTCGAC
GGTGGGCAATTGCGCCTCGGTAAGCTTTGCCGCGTAGCCTTTTCATC
Clone Rv119
:::::::::::::::Rv119SP6.seq:::::::::::::::
ATACTCAAGCTTCACTGACAAGGGACGAATTCGTCGGCCGCCTGTTCGACTGGGTGGTGGCCGAGCTGGTCGCCACCACTCAGGCCGCGGTCACGGCGGTACCGGC
GCGGGAGCAAACTCGCGCGGGCATGGCCAACTTCTTGCGGACCATCACCGCAGACGCCCGCTTCGGACCCCTGCTGTCCACCACACAGTTGGCCAACGCATTAATC
ACCCGCAAGCTTGCGGAATCCACCGCCCTGTTCGC
:::::::::::::::Rv119T7.seq:::::::::::::::
TCCATCACCCGATGTGGCNGGAGCACTGCCATGTCGATCTCAACTACCACCTCCGGCCGTGGCGGTTGCGCGCCCCGGGGGGTCCGCGCGAACTCGACGAGGCGGT
CGGAGAAATCGCCANCACCCCGCTGAACCGCGACCACCCGCTGTGGGAGATGTACTTCGTTGAGGGGCTTGCCAACCACCGGATGCGGTGGTTGCCAAAAATTCAC
CATGCGTTGGCTGACGGTGTTGCCTCGGCAAACATGATGGCACGGGGGATGGATCTGCCGCCGGGACCGGAGGTCGGCCGCTATGTGCCTGACCCCGCTCCTACCA
AGCGGCA
Clone Rv11
:::::::::::::::Rv11SP6.seq:::::::::::::::
AGCTTTGCAGTTGCTGAGTAATGTCGGCCAACGTCACCACAACCGCGATGAATTCAATCATGCCGCCCAGGGCGGCCAACCCAATGGTGGCCGCGAGCGGCAGCTC
GATCGCAGCGCGGAGGTTGCCGGCCGCCAGTTGATTCACGAACAGGGTGAGGTCATAGGCGGGCAGGATAGTGACGAAGGCAAGACCTCCATCTGCCGTCGGAAGA
AGTATCGAG
:::::::::::::::Rv11T7.seq:::::::::::::::
AGCTTCAGAACAGGCCTGTTGTGGGCGCACCCGGCTCGCCGAGTTCTGCACGCACCGCCTCAAGTGCGGCCCGCACCGCCGGCATCTCCCGGTCAGGCAGGGCCGC
GGCCCGCGCCGCAGCGACGGCGTGTTCGCGCAGTTCGCCGTCAATGATGCTGACCTGATCGGCCACCCGGGCGTTCTCGGCGTCGTCGCGTTCACTAATCGCGGTG
CTCAGCAGCGTCTCGACAGCCACCACCCGAGTGGCGACCAGCTGCTCCACCACGGACCGCAGCGATGCCCGTC
Clone Rv120
:::::::::::::::Rv120SP6.seq:::::::::::::::
ATACTCAAGCTTCAGTTCCTCCACGACGCGTTCCCAAATGAATTTCCCGATCCCACAATCTCGGTTCAGATACAGGTCGCCATACCCCTTACTTCGGCAACGCTGG
GCGGATTGGCCCTGCCGCTGCACCAAACCATCAACGCCTTCAAATTGCCGGCAATCTCGTTCAGCCAATCCAT
:::::::::::::::Rv120T7.seq:::::::::::::::
GCTCTACGCCGCCTACGGGTCGAACATGCATCCCGAGCAGATGCTCGAGCGCGCACCCCACTCGCCGATGGCCGGAACCGGCTGGTTACCCGGGTGGCGGCTGACG
TTCGGCGGCGAGGACATCNGCTGGGAAGGGGCGCTTGCCACCGTCGTCNAAGACCCAAATTCGAAGGTGTTCGTCGTGCTCTACGACATGACCCCGGCGGACGAGA
AGAACCTTGACCGGTGGGAAGGCTCCGAGTTCGGTATCCACCAGAAGATCCGATGCCGCGTGGAGCGCATTTCCTCGGACACCACAACGGGATCCCGTCCTCG
Clone Rv121
:::::::::::::::Rv121SP6.seq:::::::::::::::
ATACTCAAGCTTGCCAAAGAGACCTCGTCCACCAAGCAGGACGCGACCGTCGAGGTGGCGATCCGGCTTGGCGTCGACCCGCGTAAGGCAAACCAGATGGTTCGCG
GCACGGTCAACCTGCCCACACCGGCACTGGTTAAGAACTGCCCGCGTCGCGGTTTTCGCGGTTGGTGAAAAGGCCAATGCCTGCGTTTGCCGTGGGGGCGGATGTT
GTCGGGAGTGACAATCTGATCAAAAGGATTCAGGGCGGTTGGCTGGAATTCAATGCCGCAATCGCGACACCGG
:::::::::::::::Rv121T7.seq:::::::::::::::
CCACGGCGTGGATCAAGGTACCGGCCGGGATGTTGCGCAATGGCAGGTTGTTGCCCGGCTTGATGTCGGCGTTAGCGCCGGATTCCACCACATCCCCTTGCGAAAG
TCCGTTGGGTGCAATGATGTAGCGCTTCTCCCCATCGAGATAGTGGAGCAACGCAATCCGTGCGGTACGGTTCGGGTCGTACTCGATGTGCGCGACCTTGGCGTTG
ACACCATCTTTGTCATTGCGGCGAAAGTCGATCATCCGGTAAGCGCGCTTATGACCGCCGCCTTTGTGCCGGGTGGTAATCCGGCCATGCGCGTTGCGTCCACCGC
GACGTGCAGCGGGCGCACCAGCGACTTCTCCGGGGTTGACCGGGTNATCTC
Clone Rv122
:::::::::::::::Rv122SP6D2.seq:::::::::::::::
GCAGCATGACGGCGGTAGCGAACACCGCCGGATGCAGCGCAAGTAGCGTCGATGTGCTCACGGAATCGCCCCGGCACCGCGATCTCGANGATCACCAGTGCCACCC
CCTGCAGCGCNACACCGACGATTCCGTACACCGCCACGCCGATCAGGCCCTGGGCCATCTGATTGGAGCTGGCGTANATGGCGGCGATGGTGACGATGGCCAGCGC
CACATACATTGTGGCGGCCAGAACCACGGCGTTGGGGCGGCGGTCGATGAACACTAGGCGACGCAGATCGCCCGGGGTCAACAGGTTGACCATCAGAAAGCCTGCG
ACTAGCACGGCGGCGCCACTAGGAAGTACAAGAANGTGGCCACCACCCCATGCAGGATCGGGGTAAGGCTGATGGTCCCGAAATCGACTCCGGCCTAATACATGAC
TCTCTCCTTTGCGTCATCGCCTTACTTGTGCGCGGAA
Clone Rv
:::::::::::::::Rv123SP6D2.seq:::::::::::::::
GGGACACACCTCGATGCTGCCGCNATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACGCATCCCGTT
GACCGGCCGGANCNCTCTCTA
:::::::::::::::Rv123T7D4.seq:::::::::::::::
TGGGCGCCTCTTTCGGCCTTCCCNNTTTAAACGNAGCANGACATTCTGGGTATCGAGTTGTACTGGATGGTGTTGGCGATGTCGGTGATCCTGCTCCTGGCGGTGG
GATCCGACTACAATCTGCTGCTGATTTCCCGGTTGAAAGAGGAAATTGGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGAC
GGCTGCCGGCATGGTGTTCGCCGTTACCATGTCGTTGTTTGTGTTCAGCGATTTGCGAATTATTGGTCAGATCGGTACCACCATCGCCTTCCC
Clone Rv124
:::::::::::::::Rv124SP6D2.seq:::::::::::::::
CCGATCGGCGCCGCANCTGGTTGGTGTTNCGGATGAATCCGCAGCGAAAATGTAGCTGCGGTGGCGTGTCGTGACTCGTNGGCGTCGACGCTCGTGGCAGCCACCG
ANCGGTTGGTCCAGGATCTGGATGGGCAAAGTTGTGCGGCCCGGCCGGTGACGGCCGATGAGCTGACCGAGGTCGACAGCGCCGTGTTGGCTGACTTGGAACCGAC
ATGGAGTCGCCCCGGTTGGCGTCACCTCAAGCATTTCAATGGTTATGCGACCAGTTTTTGGGTTACGCCGTCAGACATCACGTCGGAGACTTGGATGAGCTGTGTC
TGCCAGATAGCCCCGAATCGGGACGACCGTGGTCACGGTGCGTCTGACCACTCGGGTCGGGTCGCCCGCGCTATCGGCATGGGTGCGTNATCACAGCGACACGCGC
CTGCCCAAGGANGTNCGGNCGGACC
:::::::::::::::Rv124T7D4.seq:::::::::::::::
CGGGTTGCGGATCCACGCGTGCGGGTTGTCAGCAGCTACGGCACTGAACCGCGGCCACAGCTCGCCGATCCGCTTTCGGTGGTTCTCGATCGACTCGCCGTAGGCG
ATGCGCAGCGCCTGCTCGAATATCGGGTACACGTAGGCCGGCCTTCCCNCTTTA
Clone Rv126
:::::::::::::::Rv126SP6.seq:::::::::::::::
CTTGATTTTGATCATCATGACGATCATCACCCTAATTTTGCTACCCGCACTGGTTATCGTGGGTACCGTCGTGCTTTCCATGGGCGCCTCTTTCGGGCTTTCGGTA
TTGGTCTGGCAGGACATTCTGGGTATCGATTTGTACTGGATGGTGTTGGCGATGTCGGTGATCCTGCTCCTGGCGGTGGGATCCGACTACAATCTGCTGCTGATTT
CCCGGTTGAAAAAGGAAATTGGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGACGGCTGCCGGCATGGTGT
:::::::::::::::Rv126T7.seq:::::::::::::::
GGGGATCCCTAGATCGACCTGCAGGCATGCAAGCTTGGCGTGTCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGCGGGACACACCTCGATGCTGCCGC
CATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATC
GTCGATGACGGCATCGCCACCGGAGCNACTGTCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCAGACG
ACATCGTGGCGAGATTCGNCGGGTACGCCGATGAGGTGGTGTGTTTGGCGACGCCGGCGTNGTTCTTCGCCGNCGGGCANGGTTACCGCAACTTCACCCAGACCTC
CGACGACGAGGTGGTGGCGTCTCCTGGATCGTGCTC
Clone Rv127
:::::::::::::::Rv127SP6.seq:::::::::::::::
AAGGCTGCAGGTCGAAGCGGNTGGTTACGACTCCCTGTGTGTGATGGACCAGTTCTACTATCTGCGTCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGAGCG
GCTGCAACTGGGCGCGTTGGTGACCGGCAATACCTACCGCAGCCCCGACCCTGCTGGCAAAGATNATCACCACGCTCGACGTGGTTAGCGCCGGTCGAGCGATCCT
CGGCATTGGAGCCGGCGGGTTTGAACTGGAACACCGCCAGCTCGGCTTCGAGTCCGGCACTTCCAGTGACCGGTTCAACCGGCTCGA
:::::::::::::::Rv127T7.seq:::::::::::::::
CTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCN
GCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTACAGCCACTGCGATCGGTGCCGATCGTGG
GGAACCCACTGGCGAACCTGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGGCGACCCGGCCTATGGTTATTCGACCTCGCCGNCCAATGTTGCGAC
TCCGTTCGGGTTGTTCCAGANGTCAGCCCGGTCGTCATCGCCGACGCTCTCGTCN
Clone Rv128
:::::::::::::::Rv128SP6.seq:::::::::::::::
CGGTCATAGCCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTCGGGGTGGTCGCGACGCGCATGGGCCACCATCGCATTCACCAGGTCTGCGCGAATCACCAGCA
CGTAGACGGTTCCTTTCCTAAGCAACACCGAAGTTTCACGACCCGAATGCTCCGGGAAACATGTCACGGTAGGTCGGTATTCCGGCTACCGGCTGAGCATTGAGCA
CGCCGGCCAGCACCGCACGAGCCAGGCAATCAGCCGCCGCCGCACCGATCGCGGTGACCAGCTGAGTCTCCGGAGACAATGCGGCCGGCACGCCGGNCTCCGGCGG
CACCGCTACNGCGCCCGGTGG
:::::::::::::::Rv128T7.seq:::::::::::::::
GTGATGGCACGCCACCGCGACACCACCCGGCTGCGCTACNTCGAGCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCCTCTTTCGAGGTCG
AGGTCGATACCGATTTGCGCATCCGCANCCGCNCCCTGGACGACAGAACCGTGCCCTACGAGTGCTTGTCGGGCGGGGCCAAAGAACAGCTTGGCATCCTGGCGCG
ATTGGCCGGCGCGGCGCTGGTCGCCAAGGACGACGCCGTTCCGGTGCTGATCGACGACGCGCTGGGGTTCACCGATCCGGAGCGACTATCAAGATGGGGGAGGTCT
CTGACACCATCGGCCCCNACGGACATGTGATCGTGCCGACGTGCAGTCCCACCCCG
Clone Rv129
:::::::::::::::Rv129SP6.seq:::::::::::::::
GCGAAAGTCCGTTGGGTGCAATGATGTAGCGCTTCTCCCCATCGAGATAGTGGAGCAACGCAATCCGTGCGGTACGGTTCGGGTCGTACTCGATGTGCGCGACCTT
GGCGTTGACACCATCTTTGTCATTGCGGCGAAAGTCGATCATCCGGTNNGCGCGCTTATGACCGCCGCCTTTGTGCCGGGTGGTAATCCGGCCATGCGCGTTGCGT
CCACCGCGACCGTGCAGCGGGCGCACCAGCGACTTCTCCGGGGTTGACCGGGTGATCTCGGCGAAATCAGATACGCTGGCGCCGCGACGACCAGGCGTCGTGGGCT
TGTNCTTGCGAATTGNCATGTCTAATCANGTCTTTCTCTCACGCTCTCGTCGCCGGGCTAGGCCGCATTGCCCTGCTCCTCCTCATCGCTTCGCTCTGCATCGTCC
CCGGGCTAAGCCCGTGCCCCGAAA
:::::::::::::::Rv129T7.seq:::::::::::::::
GATGGTTCGCGGCACGGTCAACCTGCCACACGGCACTGGTAAGACTGCCCGCGTCGCGGTATTCGCGGTTGGTGAAAAGGCCGATGCTGCCGTTGCCGCGGGGGCG
GATGTTGTCGGGAGTGACGATCTGATCGAGAGGATTCAGGGCGGCTGGCTGGAATTCGATGCCGCGATCGCGAACACCGGATCAGAATGGCCAAAGTCGGTCGCAT
CGCTCGGGTGCTGGGTCCGCGCGGCCTGATGCCCAACCCGAAAACCGGCACCGTCACCGCCGACTCCCCATGGCGTCCCGGATATCAAGGGCCGGCAAATCAACTT
CCCGGTTGATCAGCAAGGCAACCTGCCTCCNCCTCCGG
Clone Rv130
:::::::::::::::Rv130SP6.seq:::::::::::::::
ATACTCAAGCTTCGTCATAAGACCATGGTGCGCTTTCTTTCACCCGTCCAGAGTCGGGGGCATCCGCACCGGCTCGCATCGCATCATCCTCCCACGACGGGCCGCT
CATCAGCTTGGGCCATTTCAATGTACTTGATACCCCGCGCTGCGGGTAGGCCACTGCGACAATTCAAACACGGTGTCACACGGTGAATAGTGTCGAGATGGGCTCT
GATCAACCGTCGCAAACCCGGTTTCGCATCAATAGCGGAATCCCACCGGGTTGCATGGAGGCTGCTGACCTTGGAAAACAAAATTTTTTCATTACAACAAAACAAC
CGCCNCGGAAACTTTGCA
:::::::::::::::Rv130T7.seq:::::::::::::::
CGAATTCGGCGTGCACCGCTATGGGTTGCAGCAGCGGCTGGCGCCGCACACCCCACTGGCCCGGGTGTTTTCGCCCCGAAGCCGGATCATGGTGAGCGAAAAGGAG
ATTCGCCTGTTCGATGCTGGGATTCGCCACCGCGAGGCCATCGACCGATTACTCGCCACCGGGGTGCGAGAGGTGCCGCAGTCCCGCTCCGTCGACGTCTCCGACG
ATCCATCCGGCTTCCGCCGTCGGGTGGCGGTAGCCGTCGATGAAATCGCTGCCGGCCGCTACCTGCAAGGTGATTCTGTCCCGTTGTGTCGAAGTGCCTTTCGCGA
TCGACTTTCCGTTGACCTACCGGCTGGGCGTCGGCACAACACCCCGGTGAAGGTCGTTTTTGTTGCAGTTGGGCGGAATCCGTGCTCTGGGTTACAGCCCCGAACT
CGTCACGGCGGTGCGCGCCGACGGAGTTGTTATCACCGATCCGTTGGCCGTACCGCGCCTTGGGC
Clone Rv132
:::::::::::::::Rv132SP6.seq:::::::::::::::
TCAGACTCCACCCAGCCAGCAGGCAGCGCGAAGCTGAATCCTCCAACCGGGTTGTCGATCCGGACAGGTTGGGGTGCGTTTGGGGCAATGACAGGTGGCGGCGGTG
CGTTCGGGTCGGCCGGCGGAGGTGCTGCGTTGGATCGCCCGGCTGGCATTCNGCGTGTTGGCGCGGCCGGTGGTGGGGGGCAACACAGGTGTCGGCCGGTGCAGGG
TGGCGCTGCAGCGGTCGACGGCGGCGAAGCGGCCGTTGTGGGTACCGGGGGCGCTGGCTCCGGATCGGCGTTGGCGGTCGCGGGCACCGCAACGGTCACCAAGCTG
GCGCTGGCCATCGCCGCGATAGCCAGTGCCGCCAATCGTCCCTTGCGACGTGTCAAGTNGGGGTCCACCTGATGCATGGCCAAAGAACCTACCGTGTTAACGGCNC
AACNCAAGGACCGCGCCGGTCGCN
:::::::::::::::Rv132T7seq:::::::::::::::
TTTCCGCGGTACCCGCTCAACTTTGTGTCNACCCTCAACGGCATTGCCGGCACCTACTACGTNCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAG
CGGGTCCGCTGAACAATTCGGTCCGTCCCACGAAAGAACCAGTTTTNCNTCTTTCNCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGG
GGAACCCACTGGCGAACCTGTGTTTCAACCAACACTTAGAGTGTAATTGTAAACCTGGGCTAGGGGAAACCGGCTCTAGTTTTTCCACCNTCTCCGCCCCNTGTTT
CGAATACTCCGTTCGGGTTGTCCCCAAA
Clone Rv132
:::::::::::::::Rv134SP6.seq:::::::::::::::
GCTTCCGGCTCGTATGTTGTGTGGAATTGTGACCGGATACCAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTAGTTAGGTGACACTATACAATA
CTCAAGCTTGCCGGCTGGTGGGCCGACCACTTCGATGGCACGACCCGTGAACTGCTGCCCGGCCAATTCTTCTTGGTCGCCCGGACCGATGGACCGCGGCTGGGAT
TCCAGAAGGTGCCCGATCCCGCCCCTGGGAAAAACCGCGTGCACCTCTACTTCACGACCAACGAC
:::::::::::::::Rv134T7.seq:::::::::::::::
CCGATCGACTGATGCGCCGACAACCACGCCCCAACAACTGGAATGAACCGTCGTGACCATCATCAGCACGCGGTTGTAGGCGACTTGCGACATGTTCAACCCGCCG
TACTCGGACGGAATCTTCAAACCGAAACAGCCCAGCTCGGCCAGGCCTTTCACGTACTCGTCGGGGATCTGGGCACCACGCTCGAGGACGCTGCCGTCCACGGTGT
CTAGGAATTCCCGCAGTTTGACCAGAAACGCCTCGGTTCGGGCCTCCTCGGCGTCCGACGGCTTGGGAAATGGGTGTATGAGCCCTACGGGAAACCGGCCCACAAA
GAGTTCTTTGGCGAAGGACGGTTTATCCCAACCACTTTCGCGAGATTCCTCGGCAAGGGCCCGCGCTTGCTCCTCGGTGACCTGAGTTTGCTGTGCCATCGCCGCC
TCCTCCCTGA
Clone Rv135
:::::::::::::::Rv135SP6.seq:::::::::::::::
TGCATCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAAT
ACTCAAGCTTTTACGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCGGG
TGCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACGAACGACGCCAGTCCGCTACGTAACCCCTCCGCGACTG
TCCATGGACAACAGCGCGTTCTCCACCGACCGGGCCCGGGTGTGGGGTGTTTCGGCGACCGGCAGCCAGGTGGTCCACACTGCCGACGGGCGCCGCGAGCCGTTCA
CCGACCAGGCCGCCGAGCAAGTCCGCCCGATCGCATACTCC
:::::::::::::::Rv135T7.seq:::::::::::::::
GGGGGCGCTGCTGGTATAGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGCCAGGCTATCGCACCCGGTT
ATCGGCTACGAGCAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGCCGTCAAGCATGTGT
GCCGCGGATTATCAGGACTGACCTCCTGGCTGACCGGCATGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCGGCTCGGATAGGCAGGTCAGCGA
ATTCTCGTGGCAGCTCGAAAGGGTCCTGCCGGTGCCGGTCTTTGCGCAAACGATGGCGCAGGTTACGGTCGCGCGGGGTGCGGCCTGGCGGCGGCCAGAGCAGGAG
TTCACCGATGCGCAGCTAGTGGCGACAGCGTCAGCCAAC
Clone Rv136
:::::::::::::::Rv136SP6.seq:::::::::::::::
TGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAAT
ACTCAAGCTTCCGTACAGGTCGCCTCCAACACGGCGGGGAAGCGACACCAGCCTACCGAGCTTGGAGTCCAGGACGCCAGCGGCGGCGTCGGTCTGCGTCGTGGTG
CCGCCGGGGTGGCGTTGGCTGGCAACGATCTCCACCCAGCCGGTCGGGTTACCCACGATCTCGGCATAGACGCGGGCCGAGGCCGGTGCGATACCGTATTGCGTCA
ATTGGGACGCGGTTGTGCATTCGGCTAGCTCGGTTGCCACACCCGTCAGGGGTTCGACGTTGGCGGGTTCGGCGGGCCCCAGCACCGCTGTCACCATGCCCGCCAA
GCCGACCTGCGGCGCCACCAACT
:::::::::::::::Rv136T7.seq:::::::::::::::
CGGCATGACCACCGACAGGCCCGACTGGTCGTACCACTCGAACGCCGGGGTGTTGATGTCCCAGCCGCTGAAGTCGTCCTGCGCGCGCGGCCGTCGAAGCAGGTAC
AGGGCGGGCGAGTTGGCACCACCACTTTGGAATTGGACCTTGATGTCACGGCCCATCGACGGCGACGGCACCTGCAGGTACTCCACCGGCAAGCCCGGCCGGGAAA
ATGCCCCCGCGGTCGCCGTGCCACCGACGGCGCCGACCAGACCCGACACTAGGGCCGCGCCGACGGCCCGGACCACGAGTCGACGCGACATACCCGTGACGGCGCC
ACGAACCCTGTCAACAAGCTGCATTCTTGCTTCCCTCATCCTCATCTCAACGCATCCATGCATGTTTGGGCGCATCCTGAATTANGTCAGACTGCAGGCGCTGGGC
CGGCAGTGCTCGTGTATCAACCACAACTTCGGGCGT
Clone Rv137
:::::::::::::::Rv137SP6.seq:::::::::::::::
TTCCAACCCTAATTGGCTTTCGGCCCCATCCGTGAGGACGGGGTGCGGGTGCTCAACAACAACGTCGTCCGCGGGACACACCTCTATGCTGCGGCCATGGACGCGG
TCCAACGCAAGCAGCTGATCGAGCTACAACCCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGACGG
CATCGCCACCGGAGCGACGGCCAAGGCGGCGTGCCACGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCGGATCGGCCCAAACGACATCGTGGCG
AGATTCGCCGGGTACGCCGATGAGGTGGTGTGTCTGGCGACGCCGGCGTTGTTCTTCGCCCTCGGGCAGGGTTACCGCAACTTCAC
:::::::::::::::Rv137T7.seq:::::::::::::::
CAGGCATGCAAGCTTTCCGCCGATACCCGCCATGTCGCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGGATCCCAAAGTGCGGATGATCGGGCCG
CCTACGTCGTGGTGTACCTCGTCGGTAACAACGAAACCGAAGCGTATGACTCGGTCCACGCGGTGCGGCACATGGTGGACACCACACCGCCACCGCACGGGGTGAA
GGCCTATGTCACCGGTCCGGCAGCACTCAATGCCGACCAGCCGAGGCCGGAGACAAAAGTATCGCTAAGGTCACCGCCGATCAACNAGCATGGTGATCGCAGCAAT
GTTGCTAGTGATCTATCGCTCCGTAATTACCGCGGTTCTCGTCTTGATCATGGTCGGCATCGACTCGGCCAATCCGCGGATTCATCGCCTTGCTCGCCGAACACAA
CATTTTCACCTTTCACATTTGCACCAACCTGCTCTTCTCAT
Clone Rv138
:::::::::::::::Rv138SP6.seq:::::::::::::::
CACTACTCAAGCTCTCTCNTCATTACCACCCCTGTAATTTGGGATGGGCAAAAGGCGAAGCACCGCTTGGCCACNAACGCCGGGACGGGACAATCTCGGGCGGCTA
TGGCTTCTCCCGGAAAGGCCCCAACGTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATTGGCACCAACCTGNTACCACCCTGGCCGG
GCGATGATCTGCAGCGTCGCCGCGGGTAGTCCCCGCCCGGGCGGCTACAGTCTGAAACCCCGATGACCATCGATGTGTGGATGCAGCATCCGACGCAACGGTTCCT
ACACGGCGGATATGTTCTCCTCGCTGCGCCGGTGGACCGGTGGGTATATCCCCTGAAACCGACATCCCN
:::::::::::::::Rv138T7.seq:::::::::::::::
CAGGCATGCAAGCTTTCGTCAGTTCATTGCGCCAGCAGACCAACAAGAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGA
CGGCGCGAACGACGCCAGCGACCACATTCAGCAGATGGCCAGCGCGTGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGGCGTGATCGACATC
GTCACCGCCGCACCACTGCCCGGCCTCGGGTTCACGCAGCCGTTGCCGCCCGCAGCGGACGATCACATCGCCGCGATCGCCCTGTTCGGGAATCCCTCGGGCCGCG
CTGGCGGGCTGATGAGCGCCCTGACCCCTCAATTCGGGTCCAAGAACATCAACCTCTGCAACAACGGCGACCCATTTGTTCGGACGGCAACCGGTGGCAACGCACC
TAAGCTACTTGCCCGGGATGA
Clone Rv139
:::::::::::::::Rv139SP6.seq:::::::::::::::
GTTTATGCACTGGTTAGGTGTTTCCATGAGTTTCATTCTGAACATCCTTTAATCATTGCTTTGCGTTTTTTTATTAAATCTTGCAATTTACTGCAAAGCAACAACA
AAATCGCAAAGTCATCAAAAAACCGCAAAGTTGTTTAAAATAAGAGCAACACGTACACAAGGAGATAAGAAGAGCACATACCTCAGTCACTTATTATCACTAGCGC
CCGCCGCAGCCGTGTAACCGAGCATAGCGAGCGAACTGGCGAGGAAGCAAAGAAGAACTGTTCTGTCAGATAGCTCTTACGCTCAGCGCAAGAAGAAATATCCACC
GTGGGGAAAAACTCCAGGTAGAGGTAC
Clone Rv13
:::::::::::::::Rv13SP6.seq:::::::::::::::
ATACTCAAGCTTGGGTGTAGCCGATCACCGGAAGTCNCATGATCAGCCACGTTCCGCGCCGCCCGGCATACGGTGGTGTACCGATCTCCGCGTCATACACCCGCGG
GTAATCGCCGACGGTGCCGGTTCGCGAGCCGAA
:::::::::::::::Rv13T7.seq:::::::::::::::
AGCTTTATCGAAAGCGCGAACAGCTCGCGGCGGCCCACGACGTGCTGCGTCGGATTGCCGGCGGCGAGATCAATTCCAGGCAGCTCCCGGACAATGCGGCTCTGCT
GGCCCGCAACGAAGGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCCGATCGCACAGGTTGGCCCACAACCGGCCGCTTGATGCCCGGTCGGCAAG
CCCGGCAGTTGCCAAACCCATCGTGATCAGGCTCGGCTCGCGAGTTCGGCGAAGAAATGGTTCGCCTGATCACCTACCATCGGCCA
Clone Rv140
:::::::::::::::Rv140SP6.seq:::::::::::::::
TCAACACGCCGCCAGCCACCACGCGCGGGTCGGGCGCCGGGCCCGGGCCTCCAGGCTNCTCCGCTCGGTGATGGCACGCCACCGCGACACCACCCGGCTGCGCTAC
GTCGAGCCATACCGGGCGGAGCTACATCGGCCCGGCCGCCCAGTGTTCGGGCCCTCTCGCCCAGGTCGAGGTCGACACCGATTTGCGCATCCGCAGCCGCACCCTG
CGACGACAGAACCGCGGCCCTACCCACTGCTTGTCGGGCGGGGGCCAAAGAACCAGCTTGACATCCTGCCACAATTGGCCGGCGCCCG
:::::::::::::::Rv140T7.seq:::::::::::::::
CAGGCATGCAAGCTTCACGTCCGTACGGCTCGGGTACGCTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCAC
ACCTTCAGTTGCTCACCGGAATCCAACCGGTAGAAGGTCGGCCAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCCGGCCA
GCACTCCGCAGGCTTCGTCGGGGTGGTCGCGACGCGCATGGGCCACCATCGCATTCACCAGGTCTGCGCGAATCACCAGCACGTAGACGGTTCCTTTCCTAAGCAA
CAC
Clone Rv141
:::::::::::::::Rv141SP6.seq:::::::::::::::
AATATTCAAGCTTTCGGCGGAAACGGACNCCTTGCGAACATTGATAACAAAATAGAAATCATTGATGGTTTGAGTCACCAGGCCGATCAAGCCTTCGCCGAGCCAA
ATTCCAATCAAGAGGCCCAAGCCCGTACCAATCAGCCCGGCAACGAGGGATTCCGTCNTTATCAGCCNAAATAACTGCTCTCGGGTACCACCCAAACAGCGCAATA
TGGCGAAAAACGGTCGCCGTTGCACAACATTAAATGTCTCGGTATTGTTGATTAAAAAGATACCCACCACCAGGGCAATCCAACTGAGAGGGGTTAAATTGACCGT
AAAAACCTCCCGTCATCTGTTT
:::::::::::::::Rv141T7.seq:::::::::::::::
CAGGCATGCAAGCTTGCTGCATCTTCCTGTGACTGCTCCCGAAACCTGGGGGTGTGCCTGCTGTGTATGCACGGCATACGGACATCCTTCCCCTGATACCCGCGGT
CGAACCAGCCACGTGTCCATCATCAGGGGTCAACCCCGGCCAAGGGCGACGGCACGCCAAGTTCGCCGACCGTTAACCTAGTGCTGTTAGCTTCATTTGCTGCGAG
CAAAACAGCTGGTCGGCCGTTAGGAACTGAATTGAAACTCAACCGATTTGGTGCCGCCGTAAGTGTCCTGTCTGCGGGTGCGCTGGTGTTGTCCGCGTGTGGTAAC
GACGACAATGTGACCGGGGGAGGTGCAACCACTGGCCAGGCGTCCGCGAAAGTCCATTGCNGGGGGAAGAAGACAC
Clone Rv142
:::::::::::::::Rv142IS1081.seq:::::::::::::::
GAAAGTGCCCCAAGGTGTTGGTGAAACTCGCTGGACGGTCCCCAGGATGTTGGCAGCACATTCACCGGACATGACCGGAGCAAGACCGGACATCCTCCCATACCGT
CGTCGCCGTGTACATCCGTAGCCCGTCCTGGCAGGTGCTGGGTTGAACAAAATCAGCCCAACACCTGCCACGACGAAGAAGCGGGTTGCGCTGGCATGTCTTGTCG
GCTCGGCGATCGAATTCTACGAATTCCTTATCTACGGGACCGCTGCGGCGCTGGTGTTTCCCACCGTGTTCTTCCCACACCTGGATCCCACGGTGGCCGCCGTGGC
CTCCAAGGGGACATTTGCTGTGGCGTTCCTATCCCGGCCGTTCGGCGCGGCCGTCTTTGGATACTTTGGAGACCGCCTCGGCCGCCAGAAGACCCTGGTCGCCACA
CTGTTGATCATGGGCCTGGCAACCGTGACTGTTGGGCTGGTTCCACGACAGTGGCCATCGCGC
:::::::::::::::Rv142SP6.seq:::::::::::::::
ATATTCAAGCTTTGTCACACCAAGTGTTCCGACCAANCGCTCCATCCGGCGAGTGGATACTCCCAGCAGGTAGCAGGTCGCCACCACGCTGGTCAGTGCGCGTTCA
TCTCGCTTGCGGCGCTGCAGCAGCCAGTCCGGGAAATAGCTGCCCTGGCGCAGCTTGGGGATCGCGACGTCGATGGTTGCGGCACGGGTGTCGAAATCACGGTGGC
GGTAGCCGTTGCGCTGATTGGACCGCTCATCGCTGCGTTCGCGGTAGCCCNCCCCGCACAGGGCGTCGGCTTCAGCCCCCATCCAAGGCGGCGATGAACGTCGAGA
GCAGCCCGCGCAGCAAATCCGGGCTCGCCTGTGCGAGTTGGTCAGCCAGAAGCTGCTCGGTGTCATAAGATGAGAAGAGGTCAGTGCGTCCTTTCCTTCG
:::::::::::::::Rv142T7.seq:::::::::::::::
CAGGCATGCAAGCTTTTTGAGCGTCTCGCGGGGCAGCTTCGCCGGCAATTCTACTAGCGAGAAGTCTGGCCCGATACGGATCTGACCGAAGTCGCTGCGGTGCAGC
CCACCCTCATTGGCGATGGCGCCGACGATGGCGCCTGGACCGATCTTGTGCCGCTTGCCGACGGCGACGCGGTAGGTGGTCAAGTCCGGTCTACGCTTGGGGCTTT
GCGGACGGTCCCGACGCTGGTCGCGGTTGCGCCGCCAAAGCGGCGGGTCGGGTGCCATCATGAATGCCTCACCGCCGCCGCACTGCACGGGCAGTGCCCGGGCGAT
GTCAGCCATCGGGACATCATGCTCGCGTTCATACTCCTCGACCAGTCCGCGGAACAGCTCCATTCCCGGACCGCCCAACGC
Clone Rv143
:::::::::::::::Rv143SP6.seq:::::::::::::::
ATACTCAAGCTTTTGGCTGGGTCGCCTTCCAATTCAGCGTGCACCGCTATGGGTTGCAGCAGCGGCTGGCNCCGCACACCCCACTGGCCCGGGTGTTTTCGCCCCG
AACCCGGATCATGGTGAGCGAAAAGGAGATTCNCCTGTTCGATGCTGGGATTCGCCACCGCGAGGCCATCGACCGATTACTCGCCACCGGGGTGCGAGAGGTGCCG
CAGTCCCGCTCCGTCGACGTCTCCGACGATCCATCCGGCTTCCGCCGTCGGGTGGCGGTAGCCGTCGATGAAATCGCTGCCGGCCGCTACCACAAGGTGATTCTGT
CCCGTTGTGTCCAAGTGCCTTTCGCGATCGACTTTCCGTTGACCTACCGGCTGGGGCGTCGGCACAACAGCCCGGTGAGGTCGTTTTTGTTGCAGTTGGGCGGAAT
CCGTGCTCTGGGTTACAGCCCCGAACTCGTCACGGCGGTGCGCCGCCGAC
:::::::::::::::Rv143T7.seq:::::::::::::::
CAGGCATGCAAGCTTCAACCTATTGACGCATTGTGCGAACTGACGGCGCCCGCGCATGGCCAATCCGGAAGACCATCATTGGCCAGTGGCCGGGCGCTAACAGGTT
CCAGCCCCCCACCAGTGCCGCTCGAACATGCGGTGCAACCCATTCGCAGGCCGGCAGGGAAAGCACCGCGGAAGCCGCAAAGGGCTGCAGTTCCGCGCCCAATAGT
GTCGTCCGCAACCAGATGCGCTCGAAAACCGCCGCCGGCAGTCAGCGCACCCGACGCGAGGTCGAGAGACGTCGTCAGCGCGCCCACATGGGGTGCCAATCGGCAC
GGCAGGTAGGCCGCGCGCAACCCCAACGCGTGGTGCATGCCACGGTCCGCAGGAGGCCACCACCC
Clone Rv144
:::::::::::::::Rv144SP6.seq:::::::::::::::
ATACTCAAGCTTCCCGGCCGCAGGTGACGGCGCGGCCTAGCGCCACTTGATGCCGCACCCGATCGACGGNCGTTGGTCGGGGTTGACTGGGGGCCCGGCGAGCAGG
GCGTCAACCGCGGCCCGGACGTCGGCGGCCGTCACCGGTCGGCCATTGCCCGGGCGGGAGTCGTCGAGCTGACCACGGTAGACAAGTCGGCGCTGGCCGTCGAAGA
CAAACGTGTCGGGTGTGCAGGCCGCGGAGAAGGCGCMGGCGACGTCTCGGGTTTCGTCGTAGAGATACGGGAACGTCCAGCCGTGGCGGCGGGCCTCGGCGACCAT
CTGATCGGGCCCGTCCTGCGGGTAGGTGACCACGTCCTTACTGGAGATACCGACCATCGGGACCCTTTGATCGGCGAGGTCCCGGCCGACCGTGGCCAATCCGGCG
GCGACGTGTCGCCCGTACCGGCCAGTGGTTC
:::::::::::::::Rv144T7.seq:::::::::::::::
CAGGCATGCAAGCTTTANCANCATCAACCCCGCCCCGCACCAGCACCGACACGATGTCGATGCCATCGAGGTGAATGTCGAACTGGCNCAAACCATCTGGCGACCG
CGACCACCGGCAACATGGGTACCGGCGATTTCCGGTGCCAATGCCGACCCGACGGGCCGCTCTCACCGCAGGTGACCTCGATCACCGAGACCAGCCGGCCGTTATA
CTCACGCACCCCTACCGTGTCACGCCCAAAACGGCGCTGGTGGTCGATTGCCGGAGTGCACCCCGCACCCAGTGTCGTGCCCGGATCCGCCGACCAATCCCGCACC
CACGTCGCCAAACCCGAAATCACCGTGATGCCGTGGTAACTGACCACCGACAGTAACGTCACTACGGCCGCCACGCCGACGCCGAACCACCACGCACATGATGATC
GGCTG
Clone Rv145
:::::::::::::::Rv145SP6.seq:::::::::::::::
ATATTCAACCTTGCACACATTGACGATACCTTGGTCACGAGACCCCAAAAGCTGGCCTCCACCGCGCGCCGGGGACCACGGTCATACCTTGANNCNGCTTTCGATC
GTTGATGCTGCGTCTTGGTCCGCGGAAACCGCAGGCTGGCATATGCACGTGGGCGCACTGGCGATCTGCGATCCCCACCGATTCGCCCGAATACAGCTTTCAGCGG
CTCCCCAAGTTGATCATCGACCGGCTGCCGGATATCCCGCACTTGCGGTGGCGGGTCACCGGCGCCCCGCTCGGACTGGACCGGCCGTGGTTCGTCGAGGACCACG
AAC
:::::::::::::::Rv145T7.seq:::::::::::::::
CAGGCATGCAAGCTTCATGCCCGCGGCATGATAGCCACATGCACGCAATCGAACTCAGCGAAACCGGCGGGCCAGGCGTCTTACGCCACCTCACCAGCGCGCAACC
TCAACCCGGCCACGGAGACCTCCTGATC
Clone Rv146
:::::::::::::::Rv146SP6.seq:::::::::::::::
ATACTCAAGCTTGATTTTGATCATCATGATGATCATCACCCGAATTGTGGTAGCCGCAGTGGTTATCGTGGGTACCGTCGTGCTTTCCATGGGCGCCTCTTTCGGG
CTTTCCGTATTGGTCTGGCAGGACATTCTGGGTATCGAGTTGTACTGGATGGTGTTGGCGATGTCGGTGATCCTGCTCNTGGCGGTGGGATCCGACTACAATCTGC
TGCTGATTTCCCGGTTGAAAGAGGAAATTGGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGACGGCTGCCGGCATGGTGTT
CGCCGTTACCATGTCGTTGTTTGTGTTCAGCGATTTGCGAATTATTGGTCAGATCGGTACCACCATCGGCTGGGCTTGCTGTTCGACAGCCTCGTCGTGCCTCGT
TCATGAAACCGTCCATTGCTGCCCTGCTGGGACCTGGTTCTGGTGGCCGCTACGGGTGCGCCCGCGCCCGGCAGTCAAATCTTCCGCCG
:::::::::::::::Rv146T7.seq:::::::::::::::
CAGGCATGCAAGCTTGGCGTGCCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACACC
TCGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCG
GATCGCGGTGATCGTCGATGACGGCATCGCCACCGGAGCGACGGCCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAACTTGGTGCTGGCGGTCCCC
ATCGGCCCAGACGACATCGTGGCGAGA
Clone Rv147
:::::::::::::::Rv147SP6.seq:::::::::::::::
ATACTCAAGCTTTTACGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCG
GGTGCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACGAACGACGCCAGTCCGCTACGTAACCCCTCCGCGAC
TGTCCATGGACAACAGCGCGTTCTCCACCGACCGGGCCCGGGTGTGGGGTGTTTCGGCGACCGGCAGCCANGTGGTCCACACTGCCGAAG
:::::::::::::::Rv147T7.seq:::::::::::::::
TAGTCGCTGACCGGTGCAGGTTTCGACNATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGCGCAGGCTATCGCACCCGTTATCGGCTACGAGCAAA
TCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGCCGTCTANCNTGTGTGCCGCGGATTATCAGG
ACTGACCTCCTGGCTGACCGGCATGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCGGCTCGG
Clone Rv148
:::::::::::::::Rv148SP6.seq:::::::::::::::
ATACTCAAGCTTTCCGCCGATACCCGCCATGTCGCGCACATCCAGAACTTCTGGGGGGATCCGCTGACAGCGGCGGGATCCCAAAGTGCGGATGATCGGGCCGCCT
ACGTCGTGGTGTACCTCGTCGGTAACAACGAAACCGAAGCGTATGACTCGGTCCACGCGGTGCGGCACATGGTGGACACCACACCGCCACCGCACGGGGTGAAGGC
CTATGTCACCGGTCCGGCAGCACTCAATGCCGACCAGGCCGAGGCCGGAGACAAAAGTATCGCTAAGGTCACCGCGATCACGAGCATGGTGATCGCAGCAATGTTG
CTAGTGATCTATCGCCCCGTAATTACCGCGGTTCTCGTCTTGATCATGGTCGGCATCGACCTCGGCGCAATCCGCGGATTCNTCGCCTTGCTCGCCGACCACAACA
TTTTCAGCCTTTCAACATTTGCGACAACCTGCTCGTTCTCATGGCGATTGCNGCGAAC
:::::::::::::::Rv148T7.seq:::::::::::::::
CAGGCATGCAAGCTTGGCGTGCCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCTGGACACACC
TCGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGTTCCGCCGCGGGGCGTGACCGCATCCCGTTGACCGGGCG
GATCGCGGTGATCGTCGATGCGGCATCGCCACCGGAGCGACCGGCCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCG
ATCGGCCCAGACGACATCGTGGCGAGATTCGCCGGGTACGCCGATGAAGTGGTGTTGTTGGCGACCCGGCGTTGTT
Clone Rv149
:::::::::::::::Rv149SP6.seq:::::::::::::::
ATACTCAAGCTTTGGCATTGTGCACATTTTCCACCCGTGCTCTATTAATGCTGAGCCGCTAATTGTGACCCCAGTCGGGAAACACGCGGAGCACCAAATTCACCGC
AGCGGCCGGGGCGGTTCAACTCACCATGGATCGCTCTCGTCGTCTGGTGCTGGACAATCGTCGCTGTAGCGCGTCGCGAACACCTCAGCTTCTGCTGCCGCGGCTT
CTTCCGGCGATGGTAACCCCCAGGTTTCGCCCACGGTCTTACGTAGCAGTGCGACGCGGTGTTCATCTGCATCGACCTGTTGACTCATCCTGTCAAGGATGAAGGC
GTACTGGGCCGACTGCGCCTTCTGCCGCGCCAGGTCGGCAATCACCAGGATCTCAGAAACGAGCTGCGACTCACTCTTCCAGGCCACCCTGGCCGAAAGCTCGACA
TGGTCAATCCGGCCG
:::::::::::::::Rv149T7.seq:::::::::::::::
CAGGCATGCAAGCTTGCGGGCCGGAGTGGTTTCGACGGCCGCTCGCTTCTCGGCATCGGTTTGGGCTGTCACCAGCAGTTGGTAGTTCTTCACGTACTGTTGTTCG
AGCGTCGAGCCGCCGCGCGTGTCGAGGTCGCCGGACGCGTATCCCGCCAGGCCGGTCAGGGTGCCCTTCCAGTCCACGCCGCTGTGGTCGGCGAACCGCTTATCTT
CAATCGAGACGATCGCCAGCTTCATCGTGTTGGCGATCTTGTCCGAGGGCACCTCGAACCGGCGCTGCGAGTACAGCCACGCGATCGTGTTGCCCTTCGCGTCGAC
CATCGTCGATACCGCAGGCACTTGCCCCTC
Clone Rv14
:::::::::::::::Rv14SP6.seq:::::::::::::::
ATACTCAAGCTTCCCGGCGGCCAGTACCGAAAGCGCGAACAGCTGGCGGCAGCCCACGACGTGCTGCGTCGGATTGCCGGCGGCGAAATCAATTCCAGGCAGCTCC
CGGACAATGCGGCTCTGCTGGCCCGCAACGAAGGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCCGATCGCACAGGTTGGCCCACAACCGGCCGC
TTGATGCCCGGTCGGCAAGCCCGGCAGTTGCCAAACCCAGCGTGATCAGGCTCGGCTCGCGAGTTCGGCGAAGAAGTGGCTCGCCTGATCACCTACCATCGGCCAG
GATCTGCGTGTCATCACAACGCTCGCCAAGGAGGTTGTTGTGGTGCTATCGACGGCCTTTAGCCAGATGTTCGGAATCGACTATCCGATAGTGTCCGCGCCAATGG
ACTTGATCGCCG
:::::::::::::::Rv14T7.seq:::::::::::::::
AGCTTCGGTGTAGCCGATCACCGGAAGCCGCATGATCAGCCACGTTTCGCGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCGCGGGTAATCG
CCGACGGTGCCGGTTCGCGAGCCGAAGGTGACGACGCTGATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGCTCAACGACGTCAATCACGT
TGTCGCTTTCTACGGTCACCGACCCGGTGACCGTAGTCGCCCGGTGCGCTCGGCCGAGAAGTTGCACCGCCACCACCGCGACACCGTCTTGCACGCGGACGCCACC
CCCGGATCGGTTGTTGGCCAAGGTAATTGGGTCATTCCATTTGACGGGACGCCGACCCCGCAGCCCCAGTACCGCCCACGACCACGCCGGCTGACCCACCACTGTA
CGAACACCAAGGCGACGCCGA
Clone Rv15
:::::::::::::::Rv150SP6.seq:::::::::::::::
ATACTCAAGCTTCGGTGGCTTCGCCCGCCCTGCCGGGTGGACTTCATGACAACGCGGGGGCGATTACCCCCGCTACCGCCAGCAGCATGACGGCGGTACCTAACAC
CGCCCGGATGCCTCGCACGTGCCTCGATGTGCTCACGGAATCGCCCCGGCACCGCGATCTCGAGGATCACCAGCGTTACCCCCGGCAGCGCGACACCGACAATTCC
GTACACCGCCACGCCGATCCGGCCCTGGGCCAGCTGATTGGAGCTGGCG
:::::::::::::::Rv150T7.seq:::::::::::::::
CAGGCATGCAAGCTTCCACATGTACGGATCCACGAACATCCCGTTGAACTGACAGGTGCGGCCCGGCTCGATCAGGCCGGCCACTTGTTCTACGCGGTTACCGAAG
ATCTCTTCGGTGACCTGCCCGCCGCCGGCCAGCTCGGCCCAGTGCCCGGCGTTGGCCGCCGCGGCGACGATCTTGGCGTCCACGGTGGTCCGGGTCTTGCCCGCTA
GCACGATCCGCGAGTCGGCCGGTCACCCGGGT
Clone Rv151
:::::::::::::::Rv151SP6.seq:::::::::::::::
ATACTCAAGCTTTCCAAGTCCCAAGTGTCGATCATGGCCAAAGAGCTCGACAAAGCCGTAGAGGCGTTTCGGACCCGCCCGCTCGATGCCGGCCCGTATACCTTCC
TCGCCGCCGACGCCCTGGTGCTCAAGGTGCGCGAGGCAGGCCGCGTCGTCGGGGTGCACACCTTGATCGCCACCGGCGTCAACGCCGAGGGCTACCGAAAGATCCT
GGGCATCCAGGTCACCTCCGCCGAAGACGGGGCCGGCTGGCTGGCGTTCTTCCGCGACCTGGTCGCCCGCGGCCTGTCCGGGGTCGCGCTGGTCACCAGCGACGCC
CACGCCGGCCTGGTGGCCGCGATCGGGGCCACCCTGCCCGCAGCGGCCTGGCAGCGCT
:::::::::::::::Rv151T7.seq:::::::::::::::
CAGGCATGCAAGCTTCACACGTAGGCGCCGTCGATAAATGACTCCGCCGCGCTTCGCACATCCTCGTAGCGATCCTTGGCGAGCAGGTCAACCGGGCGCTGCCCGT
CGAGGAGCCGGTTTTTGGCGTGCAGCCACTGGCCGACACCTCGGGGGGTAAGCGAATCCGAGAGCAGGAGGACGAGGTCACGAAGCTGCGCCAGCCGGTCGTACCG
CTCAGGGCGGATGTCGCCGGTCCGCCACCCGCGTACCGCCCGATCGGACACCTGTATGACCGCGGCGACGTC
Clone Rv152
:::::::::::::::Rv152SP6.seq:::::::::::::::
CGCGGCGGCGCATTACCCCCGCTACCGTCAGCAGCTTGACGGCGGTAGCGAACACCGCCGGATGCAGCGCAGGTGCGTCTATGTGCACACGGAATCGCCCCGGCAC
CGCGATCTCGAGGATCACCAGTGCCCGCCCCCTG
:::::::::::::::Rv152T7.seq:::::::::::::::
GGGATCGAGGAACAGCGCGTTGAACTGATAGGTGCGGCCCGGCTCGAGCAGGCCGGCCATTTGTTCGATGCGGTTACCGAAGATCTCTTCGGTGACCTGCCCGCCG
CCGGCCAGCTCGGCCCAGTGCCCGGCGTTGGCCGCCGCGGCGACGATCTTGGCGTCCACGGTGGTCGGGGTCATGCCCGCGAGCAGGATCGGCGAGCGGCCGGTCA
GCCGGGTGAACTTCGTCGAGAGCTTGACCCTGCCGTCGGGGAGGCGAACCACGGTCGGTGCGTATCTCGACCAGGCCCGGGCAACCTCGGGGGTGGCGCCGACGGT
GAACAGGTTGCGCTGGCCACCGCGGGTAGCCGCCGGCACTATGCCGATGCCCAGGCCGCGGATCACCGGTGCGGTCAGTCGGGTCAGGATGTCGCCCGGCCCCAGG
TCGAAGATCCAGCGGGCGCCGGCCGCGTGGACACNGGTGATCTCGTCCACCATCGACTTTCTGATCA
Clone Rv153
:::::::::::::::Rv153SP6.seq:::::::::::::::
TAACTCAAGGCTTGCGTTGAGGCCCCAGGCCCATCGACGGTTTGGCGGCCTTAAATGCACTGAGGTCGTCAATTGACCCCACAGCGGAAATGCCGACTATTCGCAG
GCCTCCTTCGCCTTGGCTGCCGGAGAGGGGCTCCGCGGGAACCGCATGCAGGTATATGACCTCGGTTTCTCGGGTGCTACCGCGTGCCTTGTCGAGGATGAACTCG
GCGTTGGAATTGTCCAGCCGGCCCAATTCATCGAGCGCAGATTCGTACACATGGCCGGCGGCGACATACGCTTCACCGTGGATCTGCTCCACACGGACCGCCCTGT
CGGGATCCTGCTCACGGGTAAAGGAACTTACNTGGCNCTCGGTGCC
:::::::::::::::Rv153T7.seq:::::::::::::::
CCTTCTGCGCCACCCACACCGTCAACGCCCGCGAAGTCGACGTCGTCCAGGCCATCGGCGGCCTCACGGATGGATTCGGCGCGGACGTGGTGATCGACGCCGTCGG
CCGACCGGAAACCTACCAGCAGGCCTTCTACGCCCGCGATCTCGCCGGAACCGTTGTGCTGGTGGGTGTGCCGACGCCCGACATGCGCCTGGACATGCCGCTGGTC
GACTTCTTCTCTCACGGCGGTGCGCTGAAGTCGTCGTGGTACGGCGATTGCCTGCCCGAAAGCGACTTCCCCACGCTGATCGACCTTGACCTGCATGGCCGGCTGC
CGCTGCAGCGGTTCGTTTCCGAACGCATCGGGCTCGAAGACGTCGAGGAGGCGTTCCACAAGATGCATGGCGGCAAGGTATTGCGTTCGGTGGTGATGTTGTGATG
GCCGCCATCGAGCGCGTCTCACCCCACGG
Clone Rv154
:::::::::::::::Rv154SP6.seq:::::::::::::::
ATACTCAAGCTTGATTTTGATCATCATGATGATCATCACCCGAAGTGTGGTAGCCGCAGTGGTTATCGTGGGTACCGTCGTGCTTTCCATGGGCGCCTCTTTCGGG
CTTTCCGTATTGGTCTGGCAGGACATTCTGGGTATCGAGTTGTACTGGATGGTGTTGGCGATGTCGGTGATCCTGCTCCTGGCGGTGGGATCCGACTACAATCTGC
TGCTGATTTCCCGGTTGAAAAAAGAAATTGGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTTACCGCTGCCGGCATGGTGTT
CGCCGTTACCA
:::::::::::::::Rv154T7.seq:::::::::::::::
ATTGNCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACACCTCGATGCTGCCGCCATGGACGCGGTCGAACGCAAG
CAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGACGGCATCGCCACCG
GAGCGACGGCCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCAGACGACATCGTGGCGAGATTCGCCGG
GTACGCCGATGAGGTGGTGTGTTTGGCGACGCCGGCGTTGTTCTTCGCCGTCGGGCAGGGTTACCGCAACTTCACCCAGACCTCCGACGAAGAAGTGGTGGCGTTT
TCTGGATCGTGCTC
Clone Rv155
:::::::::::::::Rv155SP6.seq:::::::::::::::
ATACTCAAGCTTTTCCCGTCCGTCATCGCCCAAGCGCGTGAGGCCGAAGCGGCTGGTTACGACTCCCTGTTTGTGATGGACCACTTCTACCAACTGCCCATGTTGG
GGACGCCCGACCAGCCGATGCTGGAGGCCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGAGCGGCTGCAACTGGGCGCGTTGGTGACCGGCAATACCTACCG
CAGCCCGACCCTGCTGGCAAAGATCATCACCACGCTCGACGTGGTTAGCGCCGGTCGAGCGATCCTCGGCATTGGAGCCGGTTGGTTTGAGCTGGAACACCGCCAG
CTCGGCTTCGAGTTCGGCACTTTCAGTGACCGGTTCAACCGGCTCGAANAGGCGCTACAGATCCTCGAGCCAATGGTCAAGGGTGAGCGCCAACGTTTTTCGGCGA
TTGGTACCCACCGA
:::::::::::::::Rv155T7.seq:::::::::::::::
CGGCCACCGGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCGCGGTACCCGCTCAACTTTGTGTCGAC
CCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACG
ATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCAACCAAACT
TGAAGGTGATTGTTAACCTGGGCTACGGCGACCCGGCCTATGGTTATTCGACCTCGCCGCCCAATGTTGCGACTCCGTTCGGGTTGTTCCCAGAGGTCAGCCCGGT
CGTCATCGCCGACGCTCTCGTCGCCGGGACCAGCAGGGAATCGGCGATTTCGCCTACA
Rv156
:::::::::::::::Rv156SP6.seq:::::::::::::::
ATACTCAAGCTTGGGGTGGCGCTGTCGGTCGGTGTGCTTGGCGGCGTCGGTATCAACACCGCCCACGAAATGGGGCACAAGAAGGATTCGCTGGAGCGGTGGCTGT
CCAAAATCACCCTCGCCCAGACCTGCTACGGGCACTTCTACATCGAGCACAACCGTGGCCATCACNTCCGGGTGTCCACACCGGAGGACCCGGGGTCGGCGCGGTT
CGGCGAAACGTTGTGGGAGTTCCTGCCCCGCAGTGTTATCGGCGGCTTGCGCTCGGCCGTTCATTTGGAGGCCCAACGGCTGCGTCGGCTCGGCGTCAGCCCCTGG
AATCCCATGACGTATCTGCGCAACGACGTGCNCAACNCGTGGCTGATGTCNGTGGTGTTGTGGGGTGGGC
:::::::::::::::Rv156T7.seq:::::::::::::::
TCGCCACCGCACCGCGGCGAACGCTCAAAGGCACCTACTGGCACCAAGGCCCCACACGTCACCCTGTGACCTCCTGCGCCGACCCCGCCCGAGGTCCTGGCCGTTA
CCACCGAACGGGCGAGCCGGGAGTCTGGTACGCATCGAACAAAGAGCAAGGTGCATGGGCGGAGTTGTTCCGCCACTTCGTCGATGACGGGGTCGATCCATTCGAG
GTCCGTCGCCGCGTCGGTCGAGTGGCGGTCACACTCCAGGTACTCGACCTCACAGACGAGAGGACTCGATCCCATCTAGGTGTGGACGAAACAGATCTTCTGTCCG
ACGACTACACCACCACCCAGGCCATCGCCGCCGCCCGCGATGCCAACTTCGACGCCGTACTGGCCCCGGCGGCGGCGCTCCCCGGTTGTCAAACACTTTGCCGTGT
TCGTTCACGCACTGCCCAACATCGAGCCCGA
Clone Rv157
:::::::::::::::Rv157SP6.seq:::::::::::::::
ATGAAATAAGAAGAGCACATCCCTCAGTCGGTTATCATCACTAGCGCTCGCCGCACCCGTGTAACCGATCATAGCGAGCGAACTGGCGAGGAAGCAAAGAATATCT
GTTCTGTCAGATAGCTCTTACGCTCAGCGCAAGAAGAAATATCCCCCGCGGGAACAACTCCAGGTAGAGGTACACACGCGGATAGCCAATTCAGAGTAATAAACTG
TGACACTCACACCCTCATCAATGATGACGAACTACACCCCGATATCCGGTCACATGACGAAGGGAAAGAGAAGGATATCATCTGTGACAAACTGCCCTCAAATTTG
GCTTCCTTAA
Clone Rv159
:::::::::::::::Rv159SP6.seq:::::::::::::::
ATACTCAAGCTTGTCGAACTCCTTCTTGAATACCGGCCGGCCATCCACAGATGCCCGGAAGAACTTCCAGGTACCCATGGCGGCTGGATCAGGGGGCGGCACAGTT
GGTCTTGTCCTGCCTCGAGTGGCGTCGTTGTCCGGCTTGGACGGGGCTCCGACGGTACCGGAGGGCAGCGACAAAACACTTATGCACTTGGGCGACCCGCCGAGAC
GGTGCGACACCCATCCCGACGGCACAAGCTCAGCCGCGGCCGCTCTTGTTCTTCGTCGGATCGACATTCACCCACTTCTGACCGGGCTTGGGCGAAGGAAGCAGAA
:::::::::::::::Rv159T7.seq:::::::::::::::
GGTATAGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGCGCAGGCTATCGCACCCGTTATCGGCTACGAG
CAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCTGCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGCCGTCAAGCATGTGTGCCGCGGATTAT
CAGGACTGACCTCCTGGCTGACCGGCATGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCGGCTCGGATAGCGAGGTCAGCGAATTCTCGTGGCA
GCTCGAAAGGGTCCTGCCGGTGCCGGTCTTTGCGCAAACGATGGCGCAGGTTACGGTCGCGCGGGGTGCGGCCCTGGCGGCGGCCCA
Clone Rv15
:::::::::::::::Rv15SP6D2.seq:::::::::::::::
GACACTATATNATACTCAAGCTTCAGGTCAATGTGCGCCAAGCCCTGACGCTGGCCGACCAGGCCACCGCCGCCGGANCCCTNTCTAGA
:::::::::::::::Rv15T7.seq:::::::::::::::
CTGTAGCCACCTGTTGCCATCCCCGTCATGCCCGACTCTGGTCATCTCGGATCCGCTGACACCCCGCTAAGGCTGCTCCTCTCGGTGCATTACCTCACCGACCGCG
AACNCCCCCAGCTTTACGACTATCCGGATGACGGCACCTGGTTGCCGGCTAACTTCACCGTCAGCTTGGACGGCGGCGCTACCGTCGATGGCGCCAGGGGGGCGAT
GGCCGGGCCCGGCGACCGATTCGTCNTCANCCTGTCGCGTGAACTTGCCGACGTCATCGTGGTCGGTGTGGGCACCGTGCGCATTGAGGGCTACTCCGGCGTCCGG
ATGGGTGTCGTCAAGCGCCCGCACCGGCAGGCCCGA
Clone Rv160
:::::::::::::::Rv160SP6.seq:::::::::::::::
ATACTCAAGCTTCGCACGCTCGGCGCGCGCGGTACCGCCCAGGTCGCCCAACAGATCGTCGATGTTCGCGTCGTCCGCCTCGCGCACGTGGTCTGTCACCAGTCAA
CGTTAACGCCGCCGCACATGTCCTGCGGCCGGGCAAAAACGTGAAAAACGAGCGGGCGACTGCNATGTCATGACAGCGACGGCCGCCGATGGGCCCAGGGTCTGGC
AAATTCGATCTGTGCGGCCAGTGCCAGCAGCGTCGCCTCGTCATACGGCCGGCCGACGAGTTGAACCGACATGGGCAGGCCGTCGCCGTCGAAGTCCCACGGCACC
ACGGGCGCGGGCTGGCCGGTCAGATTCCAAAATTGAAAGTACGGAACCGCTGCACCACCAA
:::::::::::::::Rv160T7.seq:::::::::::::::
ATCGTTTCGACCAGGCGCTCCATCCGGCGAGTGGATACTCCCAGCAGGTAGCAGGTCGCCACCACGCTGGTCAGTGCGGGTTCAGCTGGCTTGCGGCGCTGCAGCA
GCCAGTCCGGGAAATAGCTGCCCTGGCGCAGCTTGGGGATCGCGACGTCGATGGTTGCGGCACGGGTGTCGAAATCACGGTGGCGGTAGCCGTTGCGCTGATTGGA
CCGCTCATCGCTGCGTTCGCGGTAGCCCGCCCCGCACAGGGCGTCGGCTTCAGCCCCCATCAAGGCGGCGATGAACGTCGAGAGCAGCCCGCGCAGCAGATCCGGG
CTCGCCTGTGCGAGTTGGTCAGCCAGAAGCTGCTCGGTGTCGATAAGATGANAAGAAGTCATTGCGTTATTTCCT
Clone Rv161
:::::::::::::::Rv161SP6.seq:::::::::::::::
ATACTCAAGCTTGGGTGTTGCCGATCACCGGAAGCCGCATGATCAGCCACGTTTCGCGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCGCGG
GTAATCGCCGACGGTGCCGGTTCGCGAGCCGAAGGTGACGACGCTGATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGCTCAACNACGTCA
ATCACGTTGTCGCTTTCTACGGTCACCGACCCGGTGACCGTAGTCGCCCGGTGCGCTCGGCCGAGAAGTTGCACCGCCACCACCGCGACAACGTCTTGCACGCGGA
CGCCACCCCCCGGAT
:::::::::::::::Rv161T7.seq:::::::::::::::
GCGCMAACAGCTCGCGGCAGCCCACGACGTGCTGCGTCGGATTGCCGGCGGCGAGATCAATTCCAGGCAGCTCCCGGACAATGCGGCTCTGCTGGCCCGCAACGAA
GGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCCGATCGCACAGGTTGGCCCACAACCGGCCGCTTGATGCCCGGTCGGCAAGCCCGGCAGTTGCC
AAACCCAGCGTGATCAGGCTCGGCTCGCGAGTTCGGCGAAAAAGTGGCTCGCCTGATCACCTACCATCGGCCAGGATCTGCGTGTCATCACGACGCTCGCCAAGGA
GGTTGTTGTGGTGCTATCGACGGCCTTTAGCCAGATGTTCGGAATCGACTATCCGATAGTGTCCGCGCCAATGGACTTGATCGCCGGCGGTGAGCTGGCTGCCGCN
GT
Clone Rv162
:::::::::::::::Rv162SP6.seq:::::::::::::::
ATACTCAAGCTTTCTCCGATACCCGCCATGTCGCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGGATCCCAAAGTGCGGATGATCGGGCCGCCTA
CGTCGTGGTGTACCTCGTCGGTAACAACGAAACCGAAGCGTATGACTCGGTGCACGCGGTGCGGCACATGGTGGACACCACACCGCCACCGCACGGGGTGAAGGCC
TATGTCACCGGTCCGGCAGCACTCAATGCCGACCAGGCCGAGGCCGGAAACAAAAGTATCGCTAAGGTCAGCGCGATCACGAACATGGTGATCGCAGCAATGTTGC
TAGTGATCTATCGCTCCG
:::::::::::::::Rv162T7.seq:::::::::::::::
CCATGAGCACCGCCAGCCGAGCACGAGGCCAAACTCCGCCGACGCAGGCCGGTTGGACTTGTCGTGCTGGACAAGGGGTTTAGCCGCCGAAGCAGTGACGTACATC
GGCGAAGAGCAGTTCGCCTGTCGACCGACGGCGCAAACCGTGAGGCTAGGGAAGCGAGGAGCACATGGCCGCCGACCCGCAATGTACACGCTGCAAGCAAACCATC
GAACCCGGATGGCTATACATCACCGCCCATCGCCGCCGGTCAAGCCGGGATCGTCGATGACGGCGCAGTACTGATTCACGTGCCCGGTGAATGCCGCAGCCGGGGA
GCACTTTCCGCCAAAACTAACCCGGTTGG
Clone Rv163
:::::::::::::::Rv163SP6.seq:::::::::::::::
CGGGTGTCATTGGGCCACCGGCGGCGGCTGTCCGGGAAATGGCGGGTCCCCGGTGGTTTTGCTGAGGAGTGCTGAACCGTAGTCGAAGTGGCGGCGTCAGACTCCA
CCCAGCCAGCAGGCAGCGCGAANCTGAATCCTCCAACCGGGTTGTCNATCCGGACAGGTTGGGGTGCGTTTGGGGCAATNACAGGTGGCGGCGGTGCGTTCGGGTC
GGCCGGCGGAGGTGCTGCNTTGGATCCCCGGCTGGGCATTCGGCNTGTTGGCGGCGGCCGGTGGTGGGGGGGGCAACACGTGTCNGCGGTGCGGGTGGCCCT
:::::::::::::::Rv163T7.seq:::::::::::::::
CCAAGATCTACACCATCGAATCGACGGCGTCGCCGACTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCCATTGCCGGCACCTACTACGTGCACTC
CAACTACTTCATCCTGACGCCGGAACAANTTGACGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTG
CCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGGANACCCACTGGCGAACCTGGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGGCGACCC
GGCCTATGGTTATTCGACCTCGCCGCCCAAATGTTG
Clone Rv164
:::::::::::::::Rv165SP6.seq:::::::::::::::
AGCTTCCCGAGTTCGGCTTTGGATCAAGACCCCAGTCCGCGGGCGCGATCCGGCBGCTCGGTGACTACATCAAGCCACAAATCGACGGCTTTCGGGGTGCCGATAC
CGATGACGTGGCGGATGTCGAGTGTTGAGTTCTCGGCGGGGCGGATGCTCACCTGGCGATCACCTGCCTCTCGTTGACGATCGATCGTCTATGCCGCCGTCTCTGC
GGGAACAGGCCNCCAGTACATCGCCACAGACGGGATCCACCCGCATTTCGGCTACGGTTGCTCGTTTCGGTGTTCGGACTAGTCGGTCCTGGTGACGTGCCGGTGA
TGCGGACCGGTCCTAGCACTGACCAATGGCCAAAATGCGGGC
:::::::::::::::Rv164T7.seq:::::::::::::::
CGGGGGGCCTCTTAATAGTGTAGGAAAGAAGCTCTACATATTCAGGAGGATTCACCATGGCTCGTGCGGTCGGGATCGACCTCGGGACCACCAACTCCGTCGTCTC
GGTTCTGGAAGGTGGCGACCCGGTCGTCGTCGCCAACTCCGAGGGCTCCAGGACCACCCCGTCAATTGTCGCGTTCGCCCGCAACGGTGAGGTGCTGGTCTGCCAG
CCCGCCAAGAACCAGGCAGTGACCAACGTCGATCGCACCGTGCGCTCGGTCAAGCGACACATGGGCAGCGACTGGTCCATAGAGATTGACGGCAAGAAATACACCG
CGCCGGAGATCAGCGCCCGCATTCTGATGAAGCTGAAGCGCGACGCCGAGGCCTACCTCGGTGAGGACATTACCGACGCGGTTATCACGACGCCCGCCTACTTCAA
TGACGCCCAGCGTCAGGCCACCAAGGACCCGGCCAGATCGCCGGTCTCACGTGCTGCGG
Clone Rv165
:::::::::::::::Rv165SP6.seq:::::::::::::::
ATACTCAAGCTTCATAACAGGCCTGTTGTGGGCGCACCCGGCTCGCCGAGTTCTGCACGCACCGCCTCAAGTGCGGCCCGCACCGCCGGCATCTCCCGGTCACGCA
GGGCCGCGGCCCGCGCCGCAGCGACGGCGTGTTCGCGCAGTTCGCCGTCAATGATGCTGACCTGATCGGCCACCCGGGCGTTCTCGGCGTCTTCGCGTTCACTAAT
CGCGGTGCTCAGCAGCGTCTCGACAGCCACCACCCGAGTGGCGACCAGCTGCTCCACCACGGACCGCAGCGATGCCGTCACCTCACCCGTCCAGCGGTCCACCACG
ACACGGTCGTGCACCAGCGCGCGGGCATTCACCACCCAGGCGGTCACCGCCAGGCCGATCGCCACACCCGCCACCATCCCCGATGCAGCCAGGCCGGGAGTAAGA
:::::::::::::::Rv165T7.seq:::::::::::::::
CTGGTGCTGGACGGAGCCTAGTACAACTTCCTCTCCAATGCTCTTGCCCCGATCGCGGCGACCAGGATGACCCAGGACATCCTGCCGCCCGAAGTACTGGAAAAGC
TCACACCCGAGTTCGTCGCACCGGTGGTGGCCTACCTGTGCACCGAGGAGTGTGCCGACAACCCATCGGTGTACGTCGTCAGTGGTGGTTAGGTGCAGCGAGTTGC
GCTGTTTGGCAACGACGGCGCCAACTTCGACAAACCGCCGTCNGTACAAGATGTTGCGGCGCGGTGGGCCGAGATCNCCGATCTGTCCGGTGCGAAAATTGCTGGA
TTCAAGTTGTAGAACTAAAT
Clone Rv166
:::::::::::::::Rv166SP6.seq:::::::::::::::
ATACTCAAGCTTTTCCGGCGTCGTCCACCTGACCCAAAAAGCGCAGGTGCGCCGCCAAACGGCCCGCCTGGCCGCGCAACTGGTCGGCGTCGCCGTGGCCGACAAT
CAGTAGCTGGACATCCGGAAACCGCTGCACCACCTTCGGCAGCGCGTCAAGCAAAAACGGCCATTCC
:::::::::::::::Rv166T.seq:::::::::::::::
TTTCAGATCTCATTTTTATGACATGACTGGAGATCTGTCTAGATTGCAGCTCCTGTGAGCGTGGGTACCGGATTCAAGCCGGTCGGTCACGCCGCGGTGGTACCGG
CTTTGCGGCAGTGCTCGGCCTCGAGTTCGGCGATCGCGCGCGAAGTGCGTTTCGCGCACCAAGATCGCGGCCTAATGGCCGGCGATGACCGCGATGACCAGCGCGA
TCCAGGAAAAACCGTTCCAACCAGTGCTGGGCGGCCATCCCCG
Clone Rv167
:::::::::::::::Rv167SP6.seq:::::::::::::::
ATACTCAAGCTTCCCGACCACAAGTTGAACAGCACCGATTTCGGCGAGCACTTCGTCAACTTCCAGGGTGCCCGCACCAAGTATTTCGACAAGTATTTCCGTCGGG
CCGCCGCCGCCGGCGCGCGGCAGGTGGTCATCCTGGCGGCGGGGCTGGACTCCCGCGCGTACCGGCTGCCTTGGCCCGACGGGACCACGGTTTTTGAGCTGGACCG
CCCGCAGGTCCTTGATTTCAAGCGCGAGGTGCTCGCCAGCCACGGTGCCCAACCGCGCGCCCTGCGCCCGCGA
:::::::::::::::Rv167T7.seq:::::::::::::::
GTGTGCTGTCAATTCAGAGCTGAGCCTGATGCACTCAACTTACTGAGCATGCTAACGCTGGTCGTGCGGGTCTTGTTCCCGCGTGTCGGCAGGGCACACGCTCGGG
GCGTAGCTGGGAGAGGCCCCGGTCAAGCCCGGAGAGCAGTGCTCAGTCCGCCAGCTTGACCGACTTTCGATGAGAACGCGCTTCTCGCCGTATTGAACTGGCGTGC
TGACGGTCGCTGAGCAGCGCTCGCCGAGTGCGGCCGCTGATTCTTTCATCGAGCCAGGAGGCGCATTCGTGTTCGGCCGCCTGCGGGTCGGCCCCATCGTCGACGC
GATCCGTCACCCACTCCTCGATCAGGTCTGCCTCATCGAACGGGCCAACGGTGCTGTCGGAGTATGTGTGCGTGGGCACGGCGAGCCGGGTGCTGTGGTACACCCA
CCGTTGCATGACCAAGTTGACGCCTGACTGGCTGAGCACCGCGATCCGCTCACAGGTCGGAACGTTGGTG
Clone Rv169
:::::::::::::::Rv169SP6.seq:::::::::::::::
ATACTCAAGCTTTTGGTCTAGCCGGCCGAGCCCGATACAGGTGTCATTGGCCACCGGCGGCGGCTGTCCGGGAAATGGCGGGTCCCCGGTGGTTTTGCTGAGGAGT
GCTGAACCGTATGCGAAGTGGGCGGCGTCAGACTCCACCCAGCCAGCAGGCAGCGCGAAACTGAATCCTCCAACCGGGTTGTCGATCCGGACAGGTTGGGGTGCGT
TTGGGGCAATGACAGGTGGCGGCGGTGCGTCCGGGTCGGCCGGCGGAAGTGCTGCGTTGGGATCGCCCGGCTGGGCATTCTGCGTGTTGGCGGCGGCCGGTGGTGG
GGGGGCAACAGGTGTCTCCGGTGCGGGTGGCGCTGCACC
:::::::::::::::Rv169T7.seq:::::::::::::::
GGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACG
CCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCA
GTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCAACCAAACTTGAAGGTG
ATTGTTAACCTGGGCTACGGCGACCCGGCCTATGGTTATTCGACCTCGCCGCCCAATGTTGCGACTCCGTTCGGGTTGTTCCCAGAGGTCAGCCCGGTCGTCATCG
CCGACGCTCTCGTCGCCGGGACCCAGCACGGAAT
Clone Rv16
:::::::::::::::Rv16SP6.seq:::::::::::::::
TTCTNTCTTCCCNNATTCGTNNNTCTCNTACTACCNGGGCCNCAAAACACCTTGGCNAACGCTCAAAGGCGNTACNGGCACCAAGGCCCCACACGTCACCCTGTGA
CCTCCTGCGCCGACCCCGCCCGAGGTCCTGGCCGTTACCACTGAACGGGCGAGCCGGGAGTCTGGTACGCATCGAACAAAGAGCAAGGTGCATGGGCGGAGTTGTT
CCGCCNCTTTTTTTATGACGGGGTCGATCCATTCGAGGTCCGTCGCCGCGTCGGTCGAGTGGCGGTCACACTCCAGGTACTCGACCTCNCAGACGAGAGGACTCGA
TCCCATCTANGTGTGGACNAAACAGATCTTCTGTCCGACGACTACACACCACCCAGGCCATCGCCGCCGCCCGCGATGCCAACTTCNACNCCGTNCTGGCCCCGGC
GGCGGCGCTCCCCGGTTGTCAAACACCTGCCGTGTTCGTTCACNCACTGCCCAACATCNAGCCCGANCNATCCNAGGTCCGTCCAACGCCTCCGCGGCTCNCCAAC
CTNCTCCCNCTGATCNTCCGCACCAAACACATGCCCGACTCCNTGCNCCNATTGCTTGNATCCCT
:::::::::::::::Rv16T7.seq:::::::::::::::
CCGCTATCGGTCGGTGTGCTTGGCGGCGTCGGTATCAACACCGCCCACGAAATGGGGCACAAGAAGGATTCGCTGGAGCGGTGGCTGTCCAAGATCACCCTCGCCC
AGACCTGCTACGGGCACTTCTACATCGAGCACAACCGTGGCCATCACGTCCGGGTGTCCACACCGGAGGACCCGGCGTCGGCGCGGTTCGGCGAAGAGTTGTGGGA
GTTCCTGCCCCGCAGTGTTATCGGCGGCTTGCGCTCGGCCGTTCATTTGGAGGCCCAACGGCTGCGTCGGCTCGGCGTCAGCCCCTGGAATCCCATGACGTATCTG
CGCAACGACGTGCTCAACGCGTGGCTGATGTCGGTGGTGTTGTGGGGTGGGCTGATCGCGGTCTTCGGCCCGGCGCTGATCCCGTTCGTCATCATCCAGGCAGTCT
TCGGCTTCAG
Clone Rv170
:::::::::::::::Rv170SP6.seq:::::::::::::::
ATACTCATGCTTGCCGAAGTTCCGATGGGTCGCGCCGGCGANCCCAGCGAAGTCGCTAGCGTGGCCGTGTTCTTGGCTTCGGATCTATCCTCGTACATGACCGGCA
CCGTGTTGGACGTGACTGGCGGCCGGTTCATATGACACCGAGATCATTGCCACGGTACGGCAATTCGTCAAGAAGGAAATCTTTCCCAATGCACCGGCCCTCGAAC
GTGGCAACAGCTACCCGCAAGAAATCGTCGATCGGCTGGGTGTTATTGGCTTGCTCGGTCGCCGGCTGCAAGGGTATCGACACCACCGAGTTCATTCTCCGGGCGT
GCC
:::::::::::::::Rv170T7.seq:::::::::::::::
GGCGTCAACGGTGTCGGCACCGGCGTCCTGCAGTTGGTAGGCCTGCAGTTTGTGCATCAGGCCGATGCCGCGGCCCTCGTGGCCACGCATGTACAGCACCACGCCG
CGCCCCTCACGGGCGACCATCGCCAGCGCGGCGTCCAGCTGAGGCCCGCAATCGCAGCGGCGTGACCCAAACACATCGCCGGTCAAGCACTCCGAATGCACCCGGA
CCAGCACGTCGTCACCGTCGGCGTTGGGCCCGGCGATCTCGCCGCGGACCAGCGCGACATGTTGCACGTCCTCGTAGATGCTGGTGTAGCCGATGGCGCGAATCTC
CCATGACGAGTCGGAATCCGCGCCTCGGCG
Clone Rv171
:::::::::::::::Rv171SP6.seq:::::::::::::::
ATACTCAAGCTTCGGCCTCGCTGCAGGAGTGGGAGCCGCAGGGCTGGAAATCCGAAAAACGAGCCCGGTGATCGCACTGTCGCCGATCGGGGCCGCACCTGGTGGT
GTTACCGATGAATCCGCACCCAAAATGTGGCTGCGGTGGCGTTTCTTGACTCCTTGGCGTCGACTCTTGTGGCAGCCACCGAGCGGTTGGTCCAGGATCTGGATGG
GCAAAGTTGTGCGGCCCGGCCGGTGACGGCCGATGAGCTGACCGAGGTCGACAGCGCCCGTGTTGGCTGACTTGGAACCGACATGGATCGCCCCGGTTGGCGTCAC
CTCAAGCATTTCAATGGTTAT
:::::::::::::::Rv171T7.seq:::::::::::::::
ATGCGTCACCCCGATGCGCCCAGATCGGGGCTTCGCAAATAAAGCACGAACAGGCGGGCAAAACGTCTATCTCGGAGCCGGAAGGGCAATCAGCCGACCGTCGACG
AACGACACCGGCGATAACCACTTAGGCGTTGAACGGCCGGCCCAAACATTACGCCTCCGTTGATAGGCTTTCGGTCTCTTCCCCGGTCATCCCAAGCACCTTTGCG
GCAAATTTGAACGCTTTCCTGTCCGGGCACCGGCCCCGGGCTTTGGGGTCCNTCCGA
Clone Rv172
:::::::::::::::Rv172SP6.seq:::::::::::::::
ATACTCAAGCTTCAATCGCGCCGCCACAATCCAAATATGCGTCTAGCGTCTCGATGAGCGTCGGTCCGGCATCGGCTAGGGGCCGCATCACGTCGGTATGCAGGGC
CACGATCGCCCAAGGCGTCGCCCATCAAGGGCGCGTTCGGGCAAAAATTCCCCTATCCAGCACGGGCCGCGGCGCTCCGCNCCAGCCGGCGACGGCGTTCATCCCG
GAGATCGCCTCGCTAGCGCTGCGGTGCGCCGCGGTCAGCATGGGCGCCGTGGGGCCGATGACCACCGGGGCGT
:::::::::::::::Rv172T7.seq:::::::::::::::
TTCGGCGGGTCTGTAGATTGCGGTCGGCCACCCCACAGGCACTCATGAACCGCAGCCCACGATCGATCTCGGTGG
Clone Rv173
:::::::::::::::Rv173SP6.seq:::::::::::::::
GCGCACCATCGCCAGTAGGTGCCCGTGGTCGGGCGCGTCGAGCCACCCGAGCGGAAACGCGAGTCCGAACAGCAACAGCAGGACGGGCGCAACCAGGGCGGTGACC
ATGCCCCCGGCGCTGAACATCAACCACAGGAAGGGCTCCGCCGAGCGTCCGCGCGACC
:::::::::::::::Rv173T7.seq:::::::::::::::
CATCGTCGAACTTCGGTCCGGGTTGNTAGNACCGCAGCACCAAACGCACCCACCGACCCCCACGCTTCACGCCAACCCTTTAGTTCATTGGCGTGAACAGCAGCGT
AGCCGGTTGCCCCGATATATGTGGAAAAATCGTTCGGACGTACAAAAAAAGTTCCTGACGCTGGCGTCAACTCGAAACTGCCTCGGAAGTCAATGATGATCCATCA
GTCAATATTAAAGTCG
Clone Rv174
:::::::::::::::Rv174SP6.seq:::::::::::::::
ATACTCAAGCTTGTCTGCTGCCTCAGCGTATGCATGCAACAGCGCATCGCGATCAACGATCAGGCGCGCCGATTTCGGGCCGCGGGCAGTGGCACTGGCCAGATGG
CCGTTTTTTTCGAGAAACTTCAACGCCTGAGCGCTGCTTCCCATCGAGAGACCGGTGGCCTCTACAACCGATGCGACAGTTGGACCGGCGATGTTCGCCAGCAGCG
CTTCACATACGGCAAGTNTGGCGCGG
:::::::::::::::Rv174T7.seq:::::::::::::::
TTGTCCAGGCGGGGAATCGGGCAGGGAGACGACACCTTCGTTCGGTTCGATCGTCGCGAACGGGTAGTTGGCCGCGACCACGTTGTTTCGGGTCAGCGCGTTGAAA
AGTGTCGACTTGCCGACGTTGGGCAGGCCCACGATCCCCAGGCTCAAGCTCACAGA
Clone Rv175
:::::::::::::::Rv175SP6.seq:::::::::::::::
ATACTCATGCTTGGCGCCTGGGTGGCAGCCCACCTGCCCACCACACGGACCGCGGTGCGGACGCGGCTGACGCGCCTGGTGGTCAGCATCGTGGCCGGTCTGCTGT
TGTATGCCAACTTCCCGCCGCGCAACTGCTGGTGGGCGGCGGTGGTTGCGCTCGCATTGCTGGCCTGGGTGCTGACCCNCCGCNCNACAACACCGGTGGGTGGGCT
GGGCTACGGCCTGCTATTCGGCCTGGTGTTCTACGTCTCGTTGTTGCCGTGGATCGGCGAGCTGGTGGGCCCCGGGCCCTGGTTGGCACTGGCGACGACGTNCGCG
CTGTTCCCCGGCATCTTCGGTCTGTTCGCCGTCGTGGTACCCTGTTGCCGGGTTGGCCC
:::::::::::::::Rv175T7.seq:::::::::::::::
CGCCAATTCACGATATCGTTAACCGATATCCCGAGCCGATAGCTGGCGGGCTCGGGTGGTGGCCAGCGGCGCTGCGACGAAAGGTGTGACCGTCATGAAACAGACA
CCACCGGCGGCCGTCGGCCGTCGTCACCTGCTCGAGATCTCAGCATCCGCAGCCGGTGTGATCGCGCTTTCGGCGTGTAGTGGGTCGCCGCCCGACCCCGGCAAAG
GCCGGCCCGACACAACCCCGGAACAGGAAGTCCCGGTCACCGCGCCCGAAGNACTTGATGCGCGAACNCGGAGTGCTCCAAACGCATCCTGCTGAT
Clone Rv176
:::::::::::::::Rv176SP6.seq:::::::::::::::
ATACTCAAGCTTGGGCACTGACTTCGGTACCCCCTCCGCCTTTGGCCAGCAGCAGCCACAGCGCGGTTCGCGGACCGAACGTGGACATCAATAGCCCGGAATCGGT
GTGTGCAAGTTGGTAAACGGTGTTGATCCCAAGCTTTGCCAGCCTTTTCGTAGTCTTGGGCCCCACACCCCACAGTGCTTCGACGGTACGGTCACCCATGATGGCC
ATCCAGTTGGCATCGGTGAGCTGATAAATGCCAGCTGGTTTCGCCAACCCGGTAGCGATCTTGGCGCGCTGCTTGTTGTCACTGATACCTATCGAGCAAGACAGCC
CGGTTTGCGACAAAATGACTTTTCGGATCTCTTCGGCGACTTCGATGGGGTCGTCGGGA
:::::::::::::::Rv176T7.seq:::::::::::::::
AAAGTCCTGTGCCGGTTCGCTAAACACCCGGCGGACACTCAGACGGTGCTGGTGGTGCGGCATGGCACCGCGGGCAGCAAAGCGCACTTCTCCGGGGGACGACAGC
AAGCGACCGCTAGACAAGAGGGGTCGTGCGCAGGCAGAAACGTTGGTACACAGCTGCTGGCGTTCGGCGCCACCGATGTTTATGCCGCCGACCGGGTGCGCTGCCA
CCAGACGATGGAGCCACTCGCCGCGGAACTGAACGTGACCATACACA
Clone Rv177
:::::::::::::::Rv177SP6.seq:::::::::::::::
ATACTCAAGCTTGGGTTCCACGCCCGCGCAGCCACGCCGTCACCTTTCCACGAGACCTCACCTGCCGATCCGAAATGGAATCGGCCGTGACGGAATTGGCGCACCG
AACACCCAACGAGGTGGTGGCTTCGTCGCGAACCGTCACCCGAGTCGCGGCCACCGTGCGCACGGCGACGTTCTACACCCGCACCAAGATCCGAAAGCTGCAAGCT
CCCAGCACCGATCCCGACGTCATCACCGCTGCCGCCCGGCACGTCCTTGACCTATTCGAGCTGGATCGGCCCGTCCGGTTGCTGGGAGTGCGGTTAGAACTGGCCT
AGAACCGGCGGGCACACCGCNCCTGGGCGGGGCGAATTCTTGACCGCNCCGGCC
:::::::::::::::Rv177T7.seq:::::::::::::::
CGCGGTTGGCGTAGTTGGACGGGTCGCCCTCCGAGGCCAATGATGACGATGACCACGCCGATCACGATGGCCACCGAGAGGGACAACAACAGAAAGCTGACGAATC
CCTCCTTGGCGGCCGGGGCTTTGTGGTCGCCGGTCGCGATGGGCGCGAATTTACGGCCCGCTCCCCCAGGCCGCCGCGAAGCAGGGTCCCCAGCCAGTTGGCGTAG
GCGGAATTAACGATCAGCGCCACCGCGATAACCTGCCATGCCTCGGGCATATCGATGTGCGGCCAGAACAGGCCGAAC
Clone Rv178
:::::::::::::::Rv178SP6.seq:::::::::::::::
CCAACAAGAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACGACGCCAGCGACCACATTCAGCAGATGGC
CAGCGCGTGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCGTGATCGACATCGTCACCGCCGCACCACTGCCCGGCCTCGGGTTCACGCAG
CCGTTGCCGCCCGCAGCGGACGATCACATCGCCGCGATCGCCCTGTTCGGGAATCCCTCGGGCCGCGCTGGCGGGCTGATGAGCGCCCTGACCCCTCAATTCGGGT
CCAAGACCATCANCCTCTGCAACAACGGCGACCCGATTTCTTCNGACGGCAACCGGTGGCGAGCGCACCTAGGCTACGTGCCCGGGATGACCAACCAGGCGGCGCG
TTTCGTCGCGAGCAGGATCTAACCGCGAGCCGCCCATAGATTCCCG
:::::::::::::::Rv178T7.seq:::::::::::::::
TAANACCCGTGTAATTTGGGATGGGCAAAAAGGCCAAGCACCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGAAGGCCCGA
ACGTACGGCGTTTCAACACGTCGCGTCNCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGGTAGCNCCCTGGCCGGGCGATGATCTGCAGCGTCGCCGCG
GGTAGTCGCCGCCCGGGCGGCTACAGTCTGAAACGCGATGACCATCGATCTCTGGATGCAGCATCCGACGCAACGGTTCCTACACGGCGATATGTTCGCCTCGCTG
CGCCGGTGGACCGGTGGGTCTATCCCGGAGACCGACNTCCCGATCGAAGCGACCGTCTCCTCGATGGACGCCGGCGGCGTCACCCTGGGTTTGCTCACCGCCTGGC
GTGGCCCCAA
Clone Rv179
:::::::::::::::Rv179SP6.seq:::::::::::::::
GTCCGCAAAAGACTCAGCGGCCGACTTTGCTCGCAGCTGGCGGTACCGCGCCACCGATTCGATGCCGTGGTCGCGGAAGAATGCCTCCCGAAATCGCACGGCCGAC
TCCAGTTCGGCGAGCATCCGCGATGCCAGCTGCGGCTGCGCCCTGCCGGCCACGGCACCCACATGCGGCAGTTCGTCCACCTGGGCCAGCGCCCCGCCGCCGAAGT
CCAAACAATAGAACTGCACCCGGCCCGCATCGTGGGTAGCAGCCAACGCCATGATCAGCGTCCGCAGCGCGGTTGACTTGCCCGTTTGCGGTGCACCTACGACCGC
GACATTGCCTGCGGCCCCGGACAAGTCGATCGTCAGCGGCACCCN
:::::::::::::::Rv179T7.seq:::::::::::::::
CGTGGCCACGAACGCCGGAAGGGACANTCTCGGGCGGCTAGGGCTTCTCGCGGGAAGGCCCGAACGTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAA
CATTCGGGGATGGCAGCAACCTGGCAGCTACCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGGTAGTCGCCGCCCGGGCGGCTACAGTCTGAAACGCGATGACC
ATCGATGTGTGGATGCATCATCCGACGCAACGGTTCCTACACGGCGATATGTTCNCCTCGCTGCGCCGGTGGACCGGTGGGTCTATCCC
Clone Rv17
:::::::::::::::Rv17SP6.seq:::::::::::::::
ATACTCAAGCTTTGCGGCGGGCGCCGAAATGTGAACGCACCAAACCCGCCCGCTGCGGGTCGGCGGGCCACTCGACCTCGAATTTCGCCGCCGTGACCATCCAGCC
CGACGGCAGTTGGGCACCCGGCCCCCCGGTCGCGGCATAACTGTTGGCGTCGCCGTCATAAAGCTCGAACAGCACCGAAACCGACTCCACCACCGGCCGGTGCGCC
TCAAAATCCACGCCGATCTCCACATACCGGGAAAACGTCGGTGTCCCATCGGGTTTCGGCTTGCCCGCCAGCTGCACACCACCGGTGGCCTCGGCCACCTTCGCGG
CCTGAGCGCAGCTACNCATCCTGACGATCATCACCCCGCCCCCGGCTCACGCTTGGCCTCCGTGACCGCACGCATCGCCCGGTTGCGCGCACCGCGACGCCCGTAC
AGCCGCGCGCAC
:::::::::::::::Rv17T7.seq:::::::::::::::
AGCTTGCCGGGACTGCGGAACAGAAGCGGCGGTTCCTACCGCGGTGTGCGGCCGGCGCGATATCGGCCTTTTTACTAACCGAACCCGATGTGGGCTCCGATCCGGC
GCGCATGGCATCGACGGCGACGCCGATCGATGACGGCCAGGCTTACGAGCTTGAGGGTGTGAAGTTGTGGACCACCAACGGTGTGGTAGCGGACCTGCTAGTGGTT
ATGGCGCGGGTACCGCGCAGTGAAGGGCACCGAGGGGGAATCAGCGCCTTTGTCGTCGAGGCTGATTCGCCCGGGATCACCGTGGAGCGGCGCAACAAGTTCATGG
GACTGCGTGGCATCNAAAACGGCGTGACCCGGCTTCATCGCGTCNGGGTGCCCAAAGACAACTTGATCGGCA
Clone Rv180
:::::::::::::::Rv180SP6.seq:::::::::::::::
CTCAAGCTTGGCGATGCGGGCTGGCCAAAACTGGCCGGGCGGGGGTTGGCTTGTTCAATCAAGGGTGGGTTGCCG
:::::::::::::::Rv180T7.seq:::::::::::::::
CCGAAGGCCCGTTCCCGGGCGTTCAGCAAGCGATCGTCGGTTGGCCCACTGCGGGTCGAATCTTGCGGCCGCGCCGGTCGTGGAACGCCCAGGTCACCCGGCGGCG
TACC
Clone Rv181
:::::::::::::::Rv181SP6.seq:::::::::::::::
ATACTCAAGCTTTTTTCTGCTCATGAAGGTTAGATGCCTGCTGCTTAAGTAATTCCTCTTTATCTGTAAAGGCTTTTTGAAGTGCATCACCTGACCGGGCAAATAG
TTCACCGGGGTGAGAAAAAAGAGCAACAACTGATTTAGGCAATTTGGCGGTGTTGATACAGCGGGTAATAATCTTACGTGAAATATTTTCCGCATCAGCCAGCGCA
GAAATATTTCCAGCAAATTCATTCTGCAATCGGCTTGCATAACGCTGACCACGTTCATAAGCACTTGTTGGGCGATAATCGTTACCCAATCTGGATAATGCAGCCA
TCTGCTCATCATCCAGCTCGCCAACCAGAACACGATAATCACTTTCGGTAAGTGCAGCAGCTTTACGACGGCGACTCCCATCGGCAATTTCTATGACACCAGATAC
TCTTCGACCGAACGCCGGTGTCGTTGACCA
Clone Rv182
:::::::::::::::Rv182SP6.seq:::::::::::::::
CTCAAGCTTGGTGCCGACATGGCCGGGCTGGAGCCCGCGTATGGCAAGGTTCCGCTCAATGTGGTTGTGATGCAGCAGGACTACGTTCGCCTCAATCAGCTCAAAC
GTCACCCCCGTGGCGTGCTGCGCAGCATGAAGGTCGGCGCCCGCACGATGTGGGCGAAGGCAACAGGTAAAAACCTGGTCGGCATGGGTCGAGCCCTCATTGGGCC
GTTGCGGATCGGGTTGCACCGCGCCGGAGTGCCGGTCGAACTCAACACCGCCTTCACCGATCTTTTCGTCAAAAATGGCGTCGTGTCCGGGGTATAC
:::::::::::::::Rv182T7.seq:::::::::::::::
CCGAAGCGTGGGAAATCCTGACCGAATACCGCGACGTGCTGGACACTTTGGCCGGCGAGCTGCTGGAAAAGGAGACCCTGCACCGACCCGAGCTGGAAAGCATCTT
CGCTGACGTCTAAAAGCGGCCGCGGCTCACCATGTTCGACGACTTCGGTGGCCGGATCCCGTCGGACAAACCGCCCATCAAGACACCCGGGGGAGATCGCGATCGA
AACGCGGCGAAACTTGGGCC
:::::::::::::::Rv183SP6.seq:::::::::::::::
CGACTCGACAAGCATTCTTGACAGTTGTTTTGGCTCGGCATGGTTAGCCAAGGTTCTGCGGTCCCACCAGATCATCTTGGTCCGGTAGCGCTCGTCCGGGTATGCT
GCCGCCGGGATTCTCGCTGCTATTACTCCCCCCGAAAAACGCCACCGGTCCAGCGCGTGGGCCGCCGCGGTCCCCATCACAAACTGAACCCCCAAGACCCCACATG
CTTAGCGGTAGGGCGCGCGCCAAGGCGGCAGCAATCGCATCACTGCGCTGCGCGTCACTATTAACCCACCCGGACTTCACTTCCACGACCCCGAATGGCGCCCGGT
CATTGATCATCTTGCGCACCGCGGATAATCCGGGATTGCCAGCCCATTCGACTACCGCATGCGAGTCATCGGCTGACCGCAGCGGTCCGATTACCCGAGCGCCCCG
ANTACATCTCCTCCAATATCAATGGGCGCAA
Clone Rv183
:::::::::::::::Rv183T7.seq:::::::::::::::
GCGGTNTAGCTTCCCGTCGTACCGGCGACCGCCAGCCGAGAAGCTCGTTTTCCCAGTGTTGCTGGGGATTCTCACGCTGCTGCTGAGTGCGTGCCAGACCGCTTCC
GCTTCGGGTTACAACGAGCCGCGGGGCTACGATCGTGCGACGCTGAAGTTGGTGTTCTCCATGGACTTGGGGATGTGCCTGAACCGGTTCACCTACGACTCCAAGC
TGGCGCCGTCTCGTCCGCAGGTCGTTGCTTGCGATAGCCGGGAGCCCCGGATCCGCAATGACGGATTCCATGCCAACGCTCCGAGTTGCATGCGGATCGACTACGA
ATTGATCACCCAGAACCATCGGGCGTATTACTGCCTGAAGTACCTGGTGCGGGTCGGATACTGCTATCCGGCGGTGACGACCCCCGGCAAGCCGCCATCCGTGCTG
CTGT
Clone Rv184
:::::::::::::::Rv184SP6.seq:::::::::::::::
CTCAAGCTTGGGCGTGACGGCCACCGGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCGCGGTACCCG
CTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCA
ATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAAAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAA
CCTGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGGCGACCCGGCCTATGGTTATTCC
:::::::::::::::Rv184T7.seq:::::::::::::::
CGGGTGTCATTGGCCACCGGCGGCGGCTGTCCGGGAAATGGCGGGTCCCCGGTGGTTTTGCTGAGGAGTGCTGAACCGTAGTCGAAGTGGGCGGCGTCAGACTCCA
CCCAGCCAGCAGGCAGCGCGAAGCTGAATCCTCCAACCGGGTTGTCGATCCGGACAGGTTGGGGTGCGTTTGGGGCAATGACAGGTGGCGGCGGTGCGTTCGGGTC
GGCCGGCGGAGGTGCTGCGTTGGGATCGCCCGGCTGGGCATTCGGCGTGTTGGCGGCGGCCGGTGGTGGGGGGGCAACANGTGTCGCCGGTGCGGGTGGCGCTGCA
Clone Rv185
:::::::::::::::Rv185SP6.seq:::::::::::::::
NCTTGATATTGGCGTCAACGGTGTCGGCACCCGGCGTCCTGCAGTTGGTAGGCCTGCAGTTTGTCGATCAGGCCGATGCCGGGCCCTCGTGGCCACGCATGTACAG
CACCACGCCGCGCCCCTCACGGGCGACCATCGCCAGCGCGGCGTCCAGCTGAGGCCCGCAATCGCAGCGGGGTGACCCAAACACATCGCCGGTCAAGCACTCCGAA
TGCACCCGGACCAGCACGTCGTCACCGTCGGCGTTGGGCCCGGCGATCTCGCCGCGGACCAGCGCGACATGTTCCACGTCCTCGTAGATGCTGGTGTAGCCGATGG
CGCGAAACTCCCCATGACGAGTCGGAATCCGCGCCTCGGCGACCCGCTCAATGTGCTTCTCGTGCTTGCGCCGCCATTCGATCAAGTCAGCAATGGTGATCAGCGC
CAGACCGTGCTCNTCGGCG
:::::::::::::::Rv185T7.seq:::::::::::::::
CATAAGGGCCGGCGTACCCGGTACCGGCCGCGGGCCTACCACGTGCCGGAACTGGAAGCGCAGTAAGCCCTCAACGCGCCACCGCTTTGGCCCGCGCGCCCGGCGT
AGGCGCATCGGCGGTGGCCGTGGGGCGGCGCACTGCGACCTCACCAGCGGCTTTCGAGCTTTGTTCGATCAACCGGCCAGCATGGTCGAGGATGCATTCGAGACCA
TATTCGAAATTGGTTTCATCGGGGGCCCCGATCCGATGCCCCCTCCCAGTTGCGTGAGCAAGCAGCGGAGTCGTCGCGGGATCGATGGCCACGGGGTGTTCAATGG
CGGATGGTCCGCTGCCCGCCGACTGGCTCTTGCGGGAGAGCCGATCTAGCACCACCGATCCGCGCACGTGGACCGAAACCGCCGAGTAGATGTCGAAAGCGT
Clone Rv186
:::::::::::::::Rv186SP6.seq:::::::::::::::
CGTCCTTTTCCCCAAGATAGAAAGGCAGGAGAGTGTCTTCTGCATGAATATGAAGATCTGGTACCCATCCGTGATACATTGAGGCTGTTCCCTGGGGGTCGTTACC
TTCCACNAGCAAAACACGTAGCCCCTTCAGAGCCNNATCCTGAGCAANATGAACAGAAACTGAGGTTTTGTAAACGCCACCTTTATGGGCAGCAACCCCGATCACC
GGTGGAAATACGTCTTCAGCACGTCGCAATCGCGTACCAAACACATCACGCATATGATTAATTTGTTCAATTGTATAACCAACACGTTGCTCAACCCGTCCTCGAA
TTTCCATATCCGGGTGCG
Clone Rv187
:::::::::::::::Rv187SP6.seq:::::::::::::::
CTCAAGCTTCATGTCCGTACGGCTCGGGTACGCTTCCGTCGCAGTGTGCGAGTGATAAATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCACACCTTC
AGTTGCTCACCGGAATCCAACCGGTAGAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCCGGCCAGCACTC
CGCAGGCTTCGTCGGGGTGGTCGCGACACGCATGGGCCACCATCGCATTCAC
:::::::::::::::Rv187T7.seq:::::::::::::::
NCGCCGCCAGCCACCACGCGCGGGTCGGGCGCCGGGCCCGGGCCGCCAGGCTGCTCCGCTCGGTGATGGCACGCCACCGCGACACCACCCGGCTGCGCTACGTCGA
GCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCCTCTTTCGAGGTCGAGGTCGATACCGATTTGCGCATCCGCAGCCGCACCCTGGACGAC
AGAACCGTGCCCTACGANTGCTTGTCGGGCGGGGCCAAAGAACAGCTTGGCATCCTGGCGCGATTGGCCGGCGCGGCGCTGGTCTCCAAAGAAGACGCCCTTCCGG
TGCTGAT
Clone Rv188
:::::::::::::::Rv188SP6.seq:::::::::::::::
CGCCACGTTCATGGGCAACAACCCCGATCACCGGTGGAAATACGTCTTCAGCACGTCGCAATCGCGTACCAAACACATCACGCATATGATTAATTCGTCCAATTGT
ATAACCAACACGTTGCTCAACCCGTCCTCGAATTTCCATATCCGGGTGCGGTAGTCGCCCTGCTTTCTCGGCATCTCTGATAGCCTGAGAAGAAACCCCAACTAAA
TCCGCTGCTTCNCCTATTCTCCAGCGCCGGG
Clone Rv189
:::::::::::::::Rv189SP6.seq:::::::::::::::
ATACTCAAGCTTCAACCGATTGACGCATTGTGCGAACTGACGGCGCCCGCGCATGGCCAATCCGGAAGACCATCATTGGCCAGTGGGGGGGCGCTAACAGGTTCCA
GCCCCCCACCAGTGCCGCTCGAACATGCGGTGCAACCCATTCGCAGGCCGGCAGGGAAAGCACCGCGGAAGCCGCAAAGGGCTGCAGTTCCGCGCCCAATAGTGTC
GTCCGCAACCAGATGCGCTCGAAAACCGCGCCGGCAGTCAGCGCACCCGACGCGAGGTCGAGAGACGTCGTCAGCGCGCCCACATGGGGTGCCAATCGGCACGGCA
GGTAGGCCGCGCGCAACCCGAACGCGTGGTGCATGCCCACGGTCCGCAGGAGGCGCAGCACCCGCCAATGCCGAAGCCCACGAAACATCGGGCGCATCCACGCTTC
AACCTC
Clone Rv18
:::::::::::::::Rv18T7.seq:::::::::::::::
AGCTTTTGGCAGGGTCTCCTTCGAATTCGGCGTGCACCGCTATGGGTTGCAGCAGCGGCTGGCGCCGCACACCCCACTGGCCCCGGGTGTTTTGCCCCGAACCCGG
ATCATGGTGAGCGAAAAGGAGATTCGCCTGTTCGATGCTGGGATTCGCCACCGCGAGGCCATCGACCGATTACTCGCCACCGGGGTGCGAGAGGTGCCGCAGTCCC
GCTCCGTCGACGTCTCCGACGATCCATCCGGCTTCCGCCGTCGGGTGGCGGTAGCCGTCGATGAAATCGCTGCCGGCCGCTACCACAAGGTGATTCTGTCCCGTTG
TGTCGAAGTGCCTTTCGCGATCGACTTTCCGTTGACCTACCGGCTGGGGCGTCGGCACAACACCCCGGTGAGGTCGTTTTTGTTGCAGTTGGGCGGAATCCGTGCT
CTGGGTTACAGCCCGAATCGTCAC
Clone Rv190
:::::::::::::::Rv190SP6.seq:::::::::::::::
ATACTCAAGCTTTGTCACACCAACTGTTTCCACCAGGCGCTCCATCCGGCGAGTGGATACTCCCAGCAGGTAGCAGGTCGCCACCACGCTGGTCAGTGCGCGTTCA
GCTCGCTTGCGGCGCTGCAGCAGCCAGTCCGGGAAATAGCTGCCCTGGCGCAGCTTGGGGATCGCGACTTCTATGGTTGCGGCACGGGTGTCGAAATCACGGTGGC
GGTAGCCGTTGCGCTGATTGGACCGCTCATCGCTGCGTTCGCGGTAGCCCGCCGCGCACAGGGCGTCGGCTTCAGCCCCCATCAAGGCGGCGATGAACGTCGAGAG
CAGCCCGCGCAGCAGATCCGGGCTCGCCTGTGCGAGTTGGTCAGCCAGAACCTGCTCGGTGT
:::::::::::::::Rv190T7.seq:::::::::::::::
CCTTAAGCCCCGCAGGGCCCGGCACGCGCGGTACCGCCCAGGTCGCCCCAACAGTCGTCGATGTTCGCGTCGTCCGCCTCGCGCACGTGGTCTGTCACCAGTCAAC
GTTAACGCCGCCGCACATGTCCTGCGGCCGGGCAAAAACGTGAAAAACGAGCGGGCGACTGCAATGTCATGACACCGACGGCCGCCGATGGGCCCAGGGTCTGGCA
GATTCGATCTGTGCGGCCAGTGCCAGCAGCGTCGCCTCGTCATACGGCCGGCCGACGAGTTGAACCGACATGGGCAGGCCGTCGCCGTCGAAGTCCCACGGCACCA
CGGCCGCGGGCTGGCCGGTCAGATTCCAGACTTGAAAGTACGGAACCCGTGCACCACCAGCAGCAACGTCGAAACTGCACCCCGGCGTTGGTAGGCGCCGATGCCG
GGACGGGCCGGTCGCGGCGCCTGGCGTCACAACTACGTCGACATCGTCGAAGATCGACTGGATCGGCTGCTCACACCACTCGGCGGCCGCAGGCCGCCATCCGCCG
TC
Clone Rv191
:::::::::::::::Rv191SP6.seq:::::::::::::::
AGCTTTTTGAGCGTCGCGCGGGGCAGCTTCGCCGGCAATTCTACTAGCGAGAAGTCTGGCCCGATACGGATCTGACCGAAGTCGCTGCGGTGCAGCCCACCCTCAT
TGGCGATGGCGCCGACGATGGCGCCTGGACCGATCTTGTGCCGCTTGCCGACGGCGACGCGGTGGGTGGTCAAGTCCGGTCTACGCTTGGGCCTTTGCGGACGGTC
CCGACGCTGGTCGCGGTTGCGCCGCGAAAGCGGCGGGTCGGGTGCCATCAGGAATGCCTCACCGCCGCGGCACTGCACGGCCAGTGCCGCGGCGATGTCAGCCATC
GGGACATCATGCTCGCGTTCATACTCCTCGACCAGTCGGCGGAACAGCTCGATTCCCGGACCGCCCAGCGCATTGGTGATGGAATCGGCGAACTTGGCCACCCGCT
GGGTGTTGACATCCTCGACGGTGGGCAATTGCCCCCGGTAACGTTTGCCGCCT
:::::::::::::::Rv191T7.seq:::::::::::::::
CGGTCCGACCCTGTTCGACGGCTACCTGAATCAACCCGATGCCACCGCCGCGGCGTTCGACGCCGACAGCTGGTACCGCACCGGCGACGTCGCGGTGGTCGACGGC
AGTGGGATGCACCGCATCGTGGGACGCGAGTCGGTCGACTTGATCAAGTCGGGTGGATACCGGGTCGGCGCCGGTGAAATTGAAACGGTGCTGCTCGGGCATCCGG
ACGTGGCGGAGGCGGCAGTCGTCGGGGTGCCCGACGATGATCTAGGCCAGCGGATCGTTGCCTACGTAGTCGGCTCAGCGAATGTCGATGCGGACGGGCTTATCAA
CTTTGTTGCCCAACAACTTTCGGTGCACAAGCGCCCGCGCGAGGTGCGTATCGTANATGCGCTGCCGCGCAACGCCTTGGGGAAAGTGCTCCAGAACATTGCTGTC
AGAAGCTGANCTACGCGAATTATCGTGTTACGCTGGA
Clone Rv192
:::::::::::::::Rv192SP6.seq:::::::::::::::
ATACTCAAGCTTGCCGAAGTTCCGATGGGTCGCGCCGGCGAGCCCAGCGAAGTCGCTACCGTGGCCGTGTTCTTGGCTTCGGATCTATCCTCGTTCATGACCGGCA
CCGTGTTGGACGTGACTGGCGGCCGGTCCATATGACACCGAGATCATTGCCACGGTACGGCAATTCGTCAAGAAGGAAATCTTTCCCAATGCACCGGCCCTCGAAC
GTGGCAACAGCTACCCGCAAGAAATCGTCGATCGGCTGGGTGTTATTGGCTTGCTCGGTCGCCGGCTGCAAGGGTATCGACACCACCGAGTTCATTCTCGGGCGTG
CCGGCGCATTCGAGCTGGCGGTGCGCGCTGCCCAGCACCGTCATAGGTACTTGACGATGGTCCACGTCGGACGAGCGCCTCCACGTCGCTGCCGAACGGTATGCAT
GGCGGCTACGATTCTC
:::::::::::::::Rv192T7.seq:::::::::::::::
CGGTGTCGGCACCGGCGTCCTGCAGTTGGTAGGCCTGCAGTTTGTGCATCAGGCCGATGCCGCGGCCCTCGTGGCCACGCATGTACAGCACCACGCCGGGCCCCTC
ACGGGCGACCATCGCCAGCGCGGCGTCCAGCTGAGGCCCGCAATCGCAGCGGCGTGACCCAAACACATCGCCGGTCAAGCACTCCGAATGCACCCGGACCAGCACG
TCGTCACCGTCGGCGTTGGGCCCGGCGATCTCGCCGCGGACCAGCGCGACATGTTCCACGTCCTCGTAGATGCTGGTGAGCCCGATGGCGCGAAACTCCCCATGAC
GAGTCGGAATCCGCGCCTCGGCGACCCGCTCAATGTGCTTCTCGTGCTTGCGCCGCCATTCGATCAAGTCAGCAATGGTGATCAGCGCCAGACCGTGCTCATCGGC
GAACACCGCAATTCATCGGTGTTGCGCCATCGAGCCCTCATCTTTTGGCTGACGATCTCGCAAATCGCCCCCGCGGGTTGCAGCCGGCAT
Clone Rv193
:::::::::::::::Rv193SP6.seq:::::::::::::::
ATACTCAAGCTTTGGGTGAAAGCCGATCACCGGAAGCCGCATGATCAGCCACGTTTCGCGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCGC
GGGTAATCGCCGACGGTGCCGGTTCGCGAGCCGAAGGTGACGACGCTGATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGCTCAACGACGT
CAATCACGTTGTCGCTTTCTACGGTCACCGACCCGGTGACCGTNCTCGCCCGGTGCGCTCGGCCGATAAGTTGCACCGCCACCACCGCGACACCGTCTTGCACGCG
GACCCACCCCCGGATCCGTTGTTGGCC
:::::::::::::::Rv193T7.seq:::::::::::::::
AGCTTGCTGGCATCCGCTCCAGTAGCGCCCCGCGCGTGGCTTCCAGCGCCCGCAGATGCTCCATGAGCCGGCCGGTCGAGTCGGCGCCGGCGTTCACCGCCACCCG
CCAGGAGCTGGCGGCCAGCATCTCCGCCTTCACGCATTGCGCGATCACAGAGAGAATATACGTCTCATATTCGTTGGAGGTCGTCGCAGGCAATCGGTCGATGACG
GATTTGATGGCATCGAGCTGTGCTTCGGCGTAGCCCTCCAGCACGTCGGTATCGCTGTGGCGGTCCACGACGACCGCACCGGCGCGGCGGACAGCCGTCGGGTTGG
ACGMTGTGCGGCGATCAGTCCGGCCAGCTCCGCCTCGGGATCAGCGGC
Clone Rv194
:::::::::::::::Rv194SP6.seq:::::::::::::::
ATACTCAAGCTTGCTGCAGCTTCCTATGACTGCTCCCGAAACCTGGGGGTGTGCCTGCTGTGTATGCACGGCATACGGACATCCTTCCCCTGAGACCCGCGGTCGA
ACCAGCCACGTGTCCATCATCAGGGGTCAACCCGGGCCAAGGGCGACGGCACGCCAAGTTCGCCGACCGTTAACCTAGTGCTGTTAGCTTCATTTGCTGCGAGCAA
AACAGCTGGTCGGCCGTTAGGAACTGAATTGAAACTCAACCGATTTGGTGCCGCCGTAGGTGTCCTGGCTGCGGGTGCGCTGGTGTTGTCCGCGTGTGGTAACNAC
NACAATGTGACCGGGGGAGGTGCAACCACTGGCCAGGCGTCGGCGAAGGTCGATTGCGGGGGGAAGAAGAACTCAAAGCCAGTGGGTCGACGCGCAGGCCAACGC
:::::::::::::::Rv194T7.seq:::::::::::::::
AGCTTGACGCGGAGACGGACACATTGCGAACATTGATGACAAAATAGAAATCATTGATGGTTTGAGTCACCAGGCCGATCAAGCCTTCGCCGAGCCAAATTGCAAT
CAAGAGGCCCAAGCCCGTACCAATCAGCCCGGCAACGAGGGATTCCGTCATTATCAGCCAAAATAACTGCTCTCGGGTTACACCCAAACAGCGCAATATGGCGAAA
AACGGTCGCCGTTGCACGACATTAAATGTCACGGTATTGTAGATTAAAAAGATACCCACCAACAAGGCAATCAAACTGAGAGCGGTTAAATTGACCGTAAAAGCGT
CCGTCATCTGTTTGACGGTGTCCCGTTGGGTATCCGACGTTTCCATACGCACACCGGCCGGCAGTCTTTGTTGGATGCGTGTTGCAGTGGCCTCATCTTTGATGAT
CAAATCGATGTGGCTCAGTGTTCCGGGCA
Clone Rv195
:::::::::::::::Rv195SP6.seq:::::::::::::::
ATACTCAAGCTTCGGCTCAGGCGGCGCTGCTGGTAAAGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGC
GCAGGCTATCGCACCCGTTATCGGCTACGAGCAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAG
ATCGCCGTCAAGCATGTGTGCCGCGGATTATCAGGACTGACCTCCTGGCTGACCGGCATGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCCGCT
CGGATAGCGAGGTCAGCGAATTCNCNTTGGCAGTCCAAAGGGTCCTGCCGGTGCCGGTCTTTGCGCAAACNAAGGCNCAGGTTA
:::::::::::::::Rv195T7.seq:::::::::::::::
TGATCGCGCATCACCTGCTTCATAAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCGGGTGCAGGTGCTCGGGCA
GCTCGGCCGCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACGAACGACGCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAGCG
CGTTCTCCACCGACCGGGCCCGGGTGTGGGGTGTTTCGGCGACCGGCAGCCAGGTGGTCCACACTGCCGACGGGCGCCGCGAGCCGTTCACCGACCAGGCCGCCGA
GCAAGTCCGCCCGATCGCATACTCCAACCGGTTGCGGTACTGCAGGTTCAGCTGGCGTACTCCTCGTCGCGCTCGGCGAGGTCTTGCTCCAGCACGTCGCANACGG
CAG
Clone Rv196
:::::::::::::::Rv196SP6.seq:::::::::::::::
CAAAGCGCGAACTGCTCGCGGCAGCCCACGACGTGCTGCGTCGGATTGCCGGCGGCGAAATCAATTCCAGGCAGCTCCCGGACAATGCGGCTCTGCTGGCCCGCAA
CGAAGGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCCGATCGCACAGGTTGGCCCACAACCGGCCGCTTGATGCCCGGTCGGCAAGCCCGGCAGT
TGCCAAACCCAGCGTGATCAGGCTCGGCTCGCGAGTTCCGGGAAGAAGTGGCTCCGCCTGATCACCTACCATCCGCCAGGATCTGCGTGTCTTCACCACGCCCGCC
AAGGAGGTTGTTGTGGTGCTATCGACCGN
:::::::::::::::Rv196T7.seq:::::::::::::::
CCGGAAGCCGCATGATCAGCCAAGTTTCGCGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCGCGGGTAATCGCCGACGGTGCCGGTTCGCGA
GCCGAAGGTGACGACGCTGATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGCTCAACGACGTCAATCACGTTGTCGCTTTCTACGGTCACC
GACCCGGTGACCGTNGTCGCCCGGTGCGCTCGGCCGAAAANTTGCACCGCCACCACCGCGAAACCGTCTTGCACNCCGGAAGCCACCCCCGATCCGTTGTTGGGCC
AGGTTATTGGGT
Clone Rv19
:::::::::::::::Rv19SP6.seq:::::::::::::::
CCGGAACCGCCGACGGCACGGTATAACGCCTCCGCATATGGGTCGACAACCAGCGGGTCGGACTTCTGGGCTTCTAGCGTTCGCGCNGTCGCGACAAACAGCGCGG
TCGAACCGACACTCGTTGTGATGTCCTAGCTATCACGTTCGGTACGCACCCAATCGAGTCTAGCGCGGGTAGNTCAGCCCCGATCTCCANGCTCCGCCGAGCCAGG
CGC
:::::::::::::::Rv19T7.seq:::::::::::::::
CTGGTTTATGTCCCGTTGAAGTTCCATCACCCGATGTGGCGGGAGCACTGCCAGGTCGATCTCAACTACCACATCCGGCCGTGGCGGTTGCGCGGGCCGGGGGGTC
GGCGCGAACTCGACGAGGCGGTCGGAGAAATCGCCAGCACCCCGCTGAACCGCGACCACCCGCTGTGGGAGATGTACTTCGTTGAGGGGCTTGCCAACCACCGGAT
CGCGGTGGTTGCC
Clone Rv1
:::::::::::::::Rv1SP6D2.seq:::::::::::::::
CCGAGCAGTTGGGAATCGCTCTGCANCAAACCAATATTCTGCGCGACGTCGCGCGACGAGCTGGACCGATTAGGCGTACGCCTCCGNCTGGACGACACCGGGGCAC
TCGATGACCCCGACGCCTACGCTCGCAGGATATTGTTCGCCGGACCCCTCTCTAG
:::::::::::::::Rv1T7.seq:::::::::::::::
TATATAATACTCAAGCTTGCCGACGCCAACGCTCGCGCGATGTTGTTAGCCCGACCCGGCTCTTACATGGCACCGGTGCCCCACACGTCAGCCTGTGACGTCCTGC
ACCGCGACTCTTTACATAGAATGTGGATTGCCGGATTGGGGATGTCCGGCATCGCTCAATCTGTAGTCCGCGTTGTCCCGCGAGGGCCATGTGGATGGGGGGAAGG
ATCCGTGGCGTCCGGGATCACCATGGGG
Clone Rv201
:::::::::::::::Rv201SP6.seq:::::::::::::::
ATACTCAAGCTTGCCGAAGTTCCGATGGGTCGCGCCGGCGAGCCCAACGAAATCGCTAGCGTGGCCGTGTTCTTGGCTTCGGATCTATCCTCGTACATGACCGGCA
CCGTGTTGGACGTGACTGGCGGCCGGTTCATATGACACCGAGATCATTGCCACGGTACGGAAATTCGTCCAGAAGGAAATCTTTCCCAATGCACCGGCCCTCGAAC
GTGGCAACAGCTACCCGCAAGAAATCGTCAATCGGCTGGGTGTTATTGGCTTGCTCGGTCGCCGGCTGCGAGGGTTTCTACACCACCGAGTTCATTCTCGGGCGTG
CCGGCGCATTCGAACTGGCGGTGCGCGCTG
:::::::::::::::Rv201T7.seq:::::::::::::::
GCACCGGCGTCCTGCAGTTGGTAGGCCTGCAGTTTGTGCATCAGGCCGATGCCGCGGCCCTCGTGGCCACGCATGTACAGCACCACGCCGCGCCCCTCACGGGCGA
CCATCGCCAGCGCGGCGTCCAGCTGAGGCCCGCAATCGCAGCGGCGTGACCCAAACACATCGCCGGTCAAGCACTCCGAATGCACCCGGACCAGCACGTCTTCACC
GTCGGCGTTGGGCCCGGCGATCTCGCCGCGGACCAACGCGACATGTTCCACGTCCTCGTACATGCTGGTGTAGCCGATGGCGCGAAACTCCCCANGACAAGTCGGA
ATCCGCGCCTCGGCGAACCGCTCAATGTGCCTCTCGTGCTTGCGCCGCCATTC
Clone Rv204
:::::::::::::::Rv204SP6.seq:::::::::::::::
TGGTCCGTGTGCGCATACCAATACAACGCGCCGGGCACCTGACGCGGCGGCCGCAACCAATCGGTGGCCATCGCCATCTTCTGCTACCCGGTCAACGGACGCACCT
TCTCCTGGCCGACGTAGTGCGCCCACCCGCCGCCGTTGCGTCCCATCGATCCGGTCAAC
Clone Rv205
:::::::::::::::Rv205SP6.seq:::::::::::::::
GGCGTGTTGGCCACCGGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCGCGGTACCCGCTCAACTTTG
TGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTCGG
TCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAAAGCCACTGGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCA
ACCAAACTTGAAGGTGATTGTTTACCTGGGCTACGGCGACCCGGCCTATGGTTATTCGACCTCCCCGCCCAA
:::::::::::::::Rv205T7.seq:::::::::::::::
CGTCCGTGNCCCCTCAANCGCGTGNNGCCGAAGCGGCTGGTTACGACTCCCTGTTTGTGATGGACACTTCTACCAACTGCCCATGTTGGGGACGCCCGACCAGCCG
ATGCTGGAGGCCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGANCGGCTGCAACTGGGCGCGTTGGTGACCGGCAATACCTACCGCAGCCCGACCCTGCTGG
CAAAGATCATCACCACGCTCGACGTGGTTAGCGCCGGTCGAGCGATCCTCGGCATTGGAGCCGGTTGGTTTGAGCTGGAAACACCGCCAGCTCGGCTTCGAGTTCG
GCACTTTCAGTGACCGGTTCAACCGGCTCGAAGAGGCGCTACAGATCCTCCAGCCAATGGTCAAGGGTGAGCGCCCAACGTTTTTGGGCGATTGGTACACCACCGA
ATC
Clone Rv207
:::::::::::::::Rv207SP6.seq:::::::::::::::
CCGCTTCCGTGTAACCGAGCANNGCGAGCGANCTGGCGAGGAAGCAAAGAAGAACTGTTCTGTCAGATAGCTCTTACGCTCAGCGCAAGAAGAAATATCCACCGTG
GGAAAAACTCCAGGTAGAGGTACACACGCGGATAGCCAATTCAGAGTAATAAACTGTGATAATCAACCCTCATCAATGATGACGAACTATCCCCCGATATCAGGTC
ACATGACGAAGGGAAAGAGAAGGAAATCAACTGTGACAAACTGCCCTCAAATTTGGCTTCCTTAAAAATTACAGTTCAAAAAGTATGAGAAAATCCATGCAGGCTG
AAGGAAACAGCAAAACTGTGACAAATTACCCTCAGTAGGTCAGAACAAATGTGACGAACCNCCCTCAAATCTGTGACAGATAACCCTCAGACTATCCTGTCGTCAT
GGAAGTGATATCGCGGAAGGAAAATACGATNTGAGTCGTCTGGCGGCCTTTCTTTTTCTCAATGTATGAGAGCG
Clone Rv209
:::::::::::::::Rv209SP6.seq:::::::::::::::
TGACACCCAACAGAGGGCACTTAAGATGGCAATGCGGCCGCCTACCTGCACGTTTTCGCGATGTCAGAGGATGCCGAGGGAGAACAATGCGAGCACGGCCGCTGAC
NTTGCTCACCGCTTTGGCGGCGGTGACATTGGTGGTGGTTGCGGGCTGCNAGGCCCGANTCNAGGCCGAAGCATATAGCGCGGCCGACCGCATTTCGTCTCGACCG
CAAGCGCGACCTCAGCCGCAGCCGGTGGAGCTACTGCTGCGCGCCATCACGCC
:::::::::::::::Rv209T7.seq:::::::::::::::
ACGGGCGACGCTGAGGTGGGCCCGCGGCTATTCATGCTGTCGTCCACGTCCAGCGACGCACTGCGCCAGACGGCCCGCCAACTAGCCACCTGGGTGGAAGAACACC
AGGACTGCGTGGCGGCCTCGGATCTGGCCTACACGCTGGCGCGTGGCCGCGCGCACCGGCCGGTGCGCACCGCGGTGGTTGCCGCCAACCTGCCGGAGCTCGTCGA
GGGTTTGCGCGAGGTGGCCGACGGTGACCCCTCTATGACGCGGCGGTGGGACACTGTGATCTAAGACCGGTCTGGGTCTTCTCCGGGCAAGGGTCTCAGTGGGCGG
CGATGGGCACCCAATTGCTCGCCAGCGAACCAGTGTTCGCGGCCACCATCG
Clone Rv20
:::::::::::::::Rv205SP6.seq:::::::::::::::
ATACTCAAGCTTCGCGAGATCCGGATGGCACTCACGCTGGACAAGACCTTCACAAAATCTGAAATCCTGACCCGATACTTGAACCTGGTCTCGTTCGGCAATAACT
CGTTCGGCGTGCAGGACGCGGCGCAAACGTACTTCGGCATCAACGCGTCCGACCTGAATTGGCAGCAAGCGGCGCTGCTGGCCGGCATGGTGCAATCGACCAGCAC
GCTCAACCCGTACACCAACCCCGACGGCGCGCTGGCCCGGCGGAACGTGGTCCTCGACACCATGATCNAAAACTTCCCGGGGAGGCGGAGGCGTTGCGTGCCGCCC
AGGGCGAACCGCTGGGGGTTCTGCCGCAGCCCAATGATTGCCGCGCGGCTGCATCGCGGGCGGCGACCGCCATTCTTCTGCGAATACGTCCAGGAGTACTGTCTCG
GGGC
:::::::::::::::Rv20T7.seq:::::::::::::::
AGCTTATGTGGCCGCCCACCTACCTTATCTAGCCTAGCTAACTAAATCCAGTGCCGACAGTGCGCGGCTGGCCACCCAGCATGAGGTTATGACCACGGCATATGCC
AGCGCGCTGGCGGCGATGCCGACGCTGACCGAGTTGGCCGCTAATCACACCAGCCATGCGGTGTTGCTGGGAACGAATTTCTTTGGAATCAATACGATCCCGATCG
CGCTCAATGAGGCCGACTATGCGCGGATGTGGATTCAGGCGGCCACCACGATGAGTATCTATGAGGGCACCTCCGATGCGGCGCTGGCGTCNGCACCGCAAACCAC
ACCGGCTCCGGTACTGTTCAACGGCGGTGCTGGCGTTTGCCAGCGCCTGCCGGCGATCTC
Clone Rv214
:::::::::::::::Rv214SP6.seq:::::::::::::::
ATACTCAAGCTTGCCACCCATGCCGAGCAAGGTCGACTCAGCGATGACGAATTGTTCTTCTTCGCGGTGTTGCTGCTGGTTGCGGGCTATGAGAGCACTGCTCATA
TGATTAGCACNTTGTTTCTGACGCTGGCCGACTATCCAGATCAGCTGACACTCCTTGCGCAGCAACCAGACCTGATCCCGTCGGCGATCGAGGAGCACCTCCGCTT
TATATCGCAATCCAAAACATCTGCCGCACAACGCGCGTCGACTATTCGGTCGGTCAAGCGGTCATCCCGGGA
:::::::::::::::Rv214T7.seq:::::::::::::::
CCGGGGTAGAACGATGCGATCTGGGCCATGTCGACATCGGTGGTACAGGTAAACCGCGCCGTGTGCGCGGTCTCGGAGATCAGAACGTGGTCGCAGTTGACACCGC
GGGCTTTCAGCCAGTCGCGATAATCGGCGAAGTCGGCGCCTGCCGCCCCAACTAGCGCGACCTCGCCACCTAGCACACCGATGGCGAAGGCCATGTTTCCGGCCAC
GCCGCCGCGGTGCATCATCAACTC
Clone Rv215
:::::::::::::::Rv215SP6.seq:::::::::::::::
ATACTCAAGCTTGGCGGCAACGCCACTACCGGGCTCACCAGGTCCTGTGCCGCCACCGCCGGCGCCGAAAGCACCATCAGGTCGTAGTTGTCTGGACGTTCGACAC
CGTAAGCGAACACAATGCCGCCGCCCATGCTGTGCCCGAGCACGATGCGCTTGCACCCGGGATATTCCCGGGTGGCGATCCCAACGAGGGTGTCGAAGTCAGCGGT
GTATCTGAGATGTCTCTCACTATCATCCGTTTGGCACCCGAGCGGGCATGCCCGCGGGGGGTCAAC
:::::::::::::::Rv215T7.seq:::::::::::::::
GTCGACGGCATCAAGGTCCGCAGTGATGGTGTTCATCTCACCCAGGAAGGCGTGAAGTGGCTGATACCGTGGCTTGAGGATTCGGTGCGGGTCGCCAGTTAATCCG
CCGTGTGCTCCGGATGAGCGCGACGGTAACCCTGGAATTGTGCTGTGTGCTGGCTGTGTCGTTGTGATGAGCCTGTCTAAGTGGTGCGTAACCGTTTGACGAGCCG
CGGCCTCGCTGCAAACATTGAAGCCCGCACGTCTGGGTTTGTATTTACACAACGAGGGCGCTCCCCGATCTGGCGCGCGCAACGAGGTGCNCACTATCCATTCGAG
GTGAACTGGACTCCTTGATGCTCATGCCGGTGCGGTTTTGTC
Clone Rv217
:::::::::::::::Rv217SP6.seq:::::::::::::::
ATACTCAAGCTTGCGTTCGATGAAGTAGTCGTCGGTCAGCGCCGCCTCTTCGAGCTCCTTGGCGATGCCCAGCAAGGAGTCATCGCCGCCGAGCTTGGCCAGGATC
TTGTCGGCCTGTTCCTTGACGATGCGGGCCCGCGGATCGTAGTTCTTGTAGACACGATGACCGAAACCCATCAATTTGACCCCGGCCTCGCGGTTCTTGACCTTGC
GTTACAAACTCGCTGACGTCGTCGCCGCTGTCGCGAATGCCCTC
:::::::::::::::Rv217T7.seq:::::::::::::::
NGTCAAGCCGAGCATGCGCGAGGNAACGACGAACCCAACAAGCCATGGTGGTTGGCGCCGTCGAGAGGTCGGCGGTCGCCACAACGGGAAGATCGCCTTGAGCGTC
GCTCGACCGCCGCCTCGAGTTGGGTCATAACGAAGTAGCTGATGCCGATCATGTCGACGTTTCCGTCGCATCAGCGTGCAGCGGCGACCCACTCGACGAGGTCTCG
GTGCCGCCGCGGCCAGGGCACCAGCAGTGACGATTCCAGGCGCCGTCGGG
Clone Rv218
:::::::::::::::Rv218SP6.seq:::::::::::::::
CGATAATCGCTTCCGGTAAGTGCAGCAGCTTTACGACGGCGACTCCCATCGGCAATTTCTATGACACCAGATACTCTTCGACCGAACGCCGGTGTCTGTTGACCAG
TCAGTAGAAAAGAAGGGATGAGATCTCCCCGTGCGTCCTCAGTAAGCAGCTCCTGGTCGCGTTCATTACCTGACCATACCCGAGAGGTCTTCTCAACACTATCACC
CCGGAGCACTTCTAGAGTAAACTTCCCATCCCGACCACATATAGGCTAAGGTAATGGGCATTACCGCGAGCCATTACTCCTACGCGCGCAATTAACGAATCCACCA
TCGGGGCCGCTGGTGTCN
Clone Rv219
:::::::::::::::Rv219SP6.seq:::::::::::::::
NAATAGTCAAGCTTTCTCGTGATTACCACCCGTGTAATTTGGGATGGGCAAAAAGGCGAATCACCGCGTGGCCACAAACGCCGGGAGGGACAATCTCGGGCGGCTA
GGGCTTCTCGCGGGAAGGCCCGAACGTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGGTATCACCCTGGCCGGG
CAATGATCTGCAGCGTCGCCGCGGGTAGTGNCCGCCCGGGCGGCTAC
:::::::::::::::Rv219T7.seq:::::::::::::::
CCAACTAGAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACGACGCCAGCGACCACATTCAGCAGATGGC
CAGCGCGTGCCGGGCCACGATGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCGTGATCGACATCGTCACCGCCGCACCACTGCCCGGTCTCGGGTTCACGCAG
CCGTTGCCGCCCGCAGCGGACGATCACATCGCCGCGATCGCCCTGTTCGGGAATCCCTCGGGGCCGCGCTGGCGGGCTGATGATCGCCCTGACCCCTCAATTCGGG
TCCAAGA
Clone Rv21
:::::::::::::::Rv21SP6.seq:::::::::::::::
ATACTCAAGCTTGCTGCAGCTTCCTGTGACTGCTCCCGAAACCTGGGGGTGTGCCTGCTGTGTATGCACGGCATACGGACATCCTTCCCCTGAGACCCGCGGTCGA
ACCAGCCACGTGTCCATCATCAGGGGTCAACCCCGGCCAAGGGCGACGGCACGCCAAGTTCGCCGACCGTTAACCTAGTGCTGTTAGCTTCATTTGCTGCGAGCAA
AACAGCTGGTCGGCCGTTAGGAACTGAATTGAAACTCAACCGATTTGGTGCCGCCCGTAAGTGTCCTGGCTGCCGGTGCGCTGGTGTT
:::::::::::::::Rv21T7.seq:::::::::::::::
AGCTTGCGCGGCGTGGCGATCGCGGTTCAAGGCGCGCTCTTCGAGCACAACGAGCGAAGACAGCTCGGCGACGGAGCCTTTATCGACATCCGTTCGGGCTGGCTGA
CCGGCGGCGAAGAACTGCTGGACGCGTTGTTGTCGACGGTGCCGTGGCGAGCCGAGCGCCGTCAGATGTACGACCGGGTGGTCGATGTGCCGCGGCTGGTGAGTTT
TCACGACCTGACCATCGAAGATCCGCCGCATCCGCAGCTGGCGCGGATGCGCC
Clone Rv220
:::::::::::::::Rv220SP6.seq:::::::::::::::
AATACTCAAGCTTGCGCACGACCAGGACGTCGAGTGGCGCTTGCAGTGACTTGGCGACCTCAAAGGCCACCGGTACCCCGCCGCGCGGCAAGCCAAGGACNACNAC
GGCCTTGCCGGATAGCTGCGCCAGGCGTTGCGCCAACTGGCGTCCAGCGTCGCCACTGATCGTCAAAGAGCTTCATCTGCCGAGTGTGCGCCATCTCATGGCTCCA
AATATGGAATTAGGTCCCTGGGCCGACTGACGACAGTCCCTCAGCGACCGGATTGCGCATCCCGCCTTGTACGCTGCTCCGCAAATCCCGGGCTTGCGTCCGCGGA
AGCGAACTCGGCGGCGCTACGGTGGTGGCTCAACTTCGGCCGTGC
:::::::::::::::Rv220T7.seq:::::::::::::::
GGTTGGTGCGGTCCACCTTCGCGGCGGCGGCGCGATATGCCTTGCTGGTCTTGCTCATTTGATATCCAATCTATGGGTCGTGGTTACTCAGCGGGCCGAAGCTGGC
CCTCCCACGGGTAGGGCCCTATTCGACGGTGATGCCCATCGACCGAGCGGTACCGGCGATGATCTTGGCCGCAGCGTCGACGTCGTTGGCGTTGAGGTCCGTCTTC
TTGGTCTCGGCGATTTCGCGGACTTGATCCCAGGTGACTTTGGCGACCTTGGTCTTGTGCGGCTCCGCCGAACCCTTCGCCACACCAGCGGCCTTAAGCAGCAGCT
TGGCGGCGGGCGGCGTCTTCAGCGTGAAAGTGAAGCTACGGTCTTCATAAACGGTGATCTCCACCGGGATGACGTTGCCGCGCTGGTTCTCCGTCGCGGCGTTGTA
CGCCTTGCAGAACTCCATGATGTTGACCCGTGCTGACCGAACGCGGGGCCCACTGGCGGGGC
Clone Rv221
:::::::::::::::Rv221SP6.seq:::::::::::::::
ATACTCAAGCTTTTCGACCCGCAAGCCGGCGGTGCCCCTCCTCGTTCCGCTGCCCGGTCTTCTCGATCGGTTCGGGGTCGCCGCGCTAGGCCCAATTGCCCGGCTC
CTCCTCGGGCCGTTCCACAACCCGCATCGTCGCCGGGCTAGGTTCAAGCCATGCCGGTAAACCCCAGGACGCCAGTGCTGATCGGCTATGGACAGGTCAACCACCG
AGGCGACATCGACGCCNAAAATCAGTCCATCGAACCCGTCGACCTGATGGCCNCCGCGGCCCGGAAAGCCGCCGAGTCCACCGTGCTCGAAGCGGTGGATTCCATC
CGTGTGGTGCACATGCTGTCGGCGCATTACCGGAATTCCCGGGCGTCTCCTCGGC
:::::::::::::::Rv221T7.seq:::::::::::::::
NCCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCGGGTGCAGGTGCTCGGGCAGCTCGGCCGCGA
CAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACGAACGACGCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAGCGCGTTCTCCACCG
ACCGGGGCCGGGTGTTGGGGTGTTCGGCAACGGCAACCAAGTTGGTCCACACTGCCGACGGGGGCCGCAAATCCGTTCACCGAACCAGGCCGCCNAAACAATTCCG
CCCGATCCCATAT
Clone Rv222
:::::::::::::::Rv222SP6.seq:::::::::::::::
ATACTCAAGCTTGTCGGGATCAATCTCGAGGGCATCCACGCACGAAAAGTAAACTCTATCAAGCTTTTTGACGACACCCACGGACGCCCCATATATGTTCGGGTGG
GCAAGAACGGTCCCTACCTGGAACGTTTGGTGGCCGGCGACACCGGTGAGCCCACGCCGCAGCGGGCCAACCTCAGCGACTCGATTACCCCGGACGAACTGACTCT
ACAGGTGGCCGAAGAGCTCTTTGCCACACCGCAACAGGGACGGACTTTGGGCTTGGACCCAGAAACCGGCCACGAAATCTTTGCCAGGGGAAGGCCGGTTTGGGCC
TTATGTTACCTATATCCTGCCGGAACCTGCGGCTGATGCGGCCGCGGCCGCTCAGGGAN
:::::::::::::::Rv221T7.seq:::::::::::::::
AGCAGCTAGCCGCGCTCGCCGCGCTGGTCGGTGCGTGCATGCTCGCAGCCGGATGCACCAACGTGGTCGACGGGACCGCCGTGGCTGCCGACAAATCCGGACCACT
GCATCAGGATCCGATACCGGTTTCAGCGCTTGAAGGGCTGCTTCTCGACTTGAGCCAGATCAATGCCGCGCTGGGTGCGACATCGATGAAGGTGTGGTTCAACGCC
AAGGCAATGTGGGACTGGAGCAAGAGCGTGGCCGACAAGAATTGCCTGGGCTATCGACGGTCCAGCACAGGAAAAGGTCTATGCCGGCACCGGGTGGACCGCTATG
CGCGGCCAACGGCTGGATGACAGCATCGATGACTCCAAGAAACGCGACCACTACGCCATTCAAGCGGTCGTCGGCTTCCCGACCGCACATGATGCCGAAGAATTCT
ACAGCTCCTCCG
Clone Rv223
:::::::::::::::Rv223IS1081N1400.seq:::::::::::::::
CGCGACTGGCTCCCCGGNCGGCTGCTCGGGTCCGCCGATAGAGACCGGGATGTCGCCCGACGACGGGCAGCCGGGTTGCGTGGGACGGGGCGGGGGTCGGGCAGCC
CAAGCAACGGGCTAGTCCCCGAATCCTACGGAGCCGTCACCTACGCCTACGTAATAGTAGCTATCAATAACAGTTGACATACGCAACGATCTGTGAGATCAATATT
GCCTGACGCATGTCAAGACAGGCGTCAAGACAGGTGTCAATAATTCGCTCCGCTGGTGACGGTAACCGGTCGTGCGGGTGTGTGACGCCTAAGGAAGGAGTGTGGG
TGGTGACGCTGAGAGTGGTTCCTGAGGGTTTGGCGGCCGCCAGTGCGGCGGTGGAGGCGTTGACCGCACGGCTGGCCGCCGCACACGCTGGCGCGGCGCCGGCGAT
TACGGCGGTGGTGGCGCCCGCGGCGGATCCGGTGTCGTTGCAGAATGCGGTGGGGTTTAGCGCCTTAAGTAGCCAGCATGCCGCGATCGCCGGCGAAAGGGTCCAA
GAACTGGGT
:::::::::::::::Rv223SP6.seq:::::::::::::::
ATACTCAAGCTTATTGAACCGCGGGTCGCAGGCAAAGTGGACCTCATAACGACTCGGGTCCAGCGACCGCGCCAACACGAACGGCCGGACGACGTGGGCCAGGGTC
GCGGCCTCCCCTACAAACAGGATCCGTTGCCTGCGAACGACAGGCTCCGGTGCGGCGTTGGGCGCCGTGCTCGTCCCAGCGTCCGGTCCCGGGTCGCCGGCGACGC
TTGTTTCCTCCATACTCGCCCCCTAATCTCGAGGCAGCCCGTACCCGCAGGCAACCTCCCAAAAATGCAATCCCGCAAAATGCAATGCGTCNAGCTATTTCTCACA
CCGACCGCTAGTTGCGGATCAGAAATCCGTTGGGCGCGGAAGTCCAGCCGAATTTGTTCTCCCGCTCCGCATCATGCTTGTAATCGTTTGGAAATTCATCCTCATA
TGCCTCGATCGCTTCATAGGGTCCAGGCCCAAACCCGGGCAGGACTGGGTGGCCGTTGATGTTGGAATCCTCCACTACTAGGTATTCACCGGC
:::::::::::::::Rv223T7.seq:::::::::::::::
GTCTCGATCATGGCCAAAGAGCTCGACGAAGCCGTAGAGGCGTTTCGGACCCGCCCGCTCGATGCCGGCCCGTATACCTTCCTCGCCGCCGACGCCCTGGTGCTCA
AGGTGCGCGAGGCAGGCCGCGTCGTCGGGGTGCACACCTTGATCGCCACCGGCGTCAACGCCGAGGGCTACCGAGAGATCCTGGGCATCCAGGTCACCTCCGCCGA
GGACGGGGCCGGCTGGCTGGCGTTCTTCCGCGACCTGGTCGCCCGCGGCCTGTCCGGGGTCGCGCTGGTCACCGGCGACGCCCACGCCGGCCTGGTGGCCGCGATC
GGCGCCACCCTGCCCGCAGCGGCCTGGCAGCGCTGCAGAACCCACTACGCAGCCAATCTGATGGCAGCCACCCCGAAGCCCTCCTGGCCGTGGGTGCGCACCCTGC
TGCACTCCATCTACGACCAGCCCGACGCCGAATCAGTTGTTGCCAATATGATCGGGTTCTCGAC
Clone Rv224
:::::::::::::::Rv224SP6.seq:::::::::::::::
ATACTCAAGCTTTCGTCAGTTCATGGCGCCAGCAGACCAACAAGAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGG
CGCGAACGACGCCAGCGACCACATTCAGCAAATGGCCAGCGCGTGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCGTGATCAAGATCTTC
ACCGCCGCACCACTGCCCGGCCTCGGGTTCACGCATCCGTTTGGCCGCCGCC
:::::::::::::::Rv224T7.seq:::::::::::::::
GCCCCGTGTAATTTGGGATGGGCAAAAAGCGAAGCACCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGAAGGCCCGAACGT
ACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGGTAGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGGTA
GTCTCCGCCCGGGCCGC
Clone Rv225
:::::::::::::::Rv225SP6.seq:::::::::::::::
ATACTCAAGCTTCCTTTGACCGAACGCGTCCACCGCACCGTGAGATTGGTGGCGCCATTCGTCGTGGTGTAGCTGCTGTTGGCGGCGTCGCCGTATTGTGCGGGCC
AGCCTTGTGCGGGGGCCGCTTCTACCCACAAGTCGGCACTTCCGCAACCGCCCAGCTCGACCGCGAATTACGGCGGCCGCAACGGCCGCCGGAAGGCGTCACGCAA
TCGCTTATCCTTTCCAGGTTCCCAAATCCTCCGCTTACTTGGGTCCTTCATCGG
:::::::::::::::Rv225T7.seq:::::::::::::::
GGCAGCGGCGACAACCGGAACGTCCGCACGGTGCTCAATCACGGGTGCACGGTGTGCATCAGAATGGCGGGGGTTCGTTGTCGCGGTGAGGCGTTCGGCGAGGAGG
TAGTGTCTACCCCTTGCCCGCGGGTTCGTGCGGACTGAAAGGGATTTCATTGGGAACCCACGGCTGCGTATCGCAGGGCCTCGGTGACGTCTGCTTCCTCNAGCTC
AGGAAGTTCGGCGAGAATCTCGGTGGATGTTATTTGGTCCGCCTAC
Clone Rv226
:::::::::::::::Rv226SP6.seq:::::::::::::::
ATACTCAAGCTTTCTCGGCTTCTCTGATAGCCTGAGAAGAAACCCCAAGTTAATCCGCTGCTTCACCTATTCTCCAGCGCCGGGTTATTTTCCTCGCTTCCGGGCT
GTCATCATTAAACTGTGCAATGGCGATAGCCTTCGTCATTTCATGACCAGCGTTTATGCACTGGTTAAGTGTTTCCATGAGTTTCATTCTGAACATCCTTTATTCA
TTGTTTTGCGTT
Clone Rv227
:::::::::::::::Rv227SP6.seq:::::::::::::::
ATACTCAAGCTTGGTGACCGGCACCGCGATACGTTGCGGCAGGCATCTGGGCTGGCGGTGGTTCGCCGCTCCGAAGCCGTCGAACACCATCGCCAGCGCGGCTTCC
ACATCAACGACCATTTCGGCCAGCTTGCGGCGCATCAGCGGCTTGTCGATGAGCGCCCCACCGAATGCCCGCCGCTGCCCGGCGTATCACATCGATTCGACCATCG
CGCGGCGCGCGTTGCCGAGGGCGAACGAGGCGGTGCCCAACCGCAATCTGTTTGGTCAGCTCCCTCATGCGGGTTGATTCCTTGCCGTCCGGACGGGCCCGCGTCA
TGCGCTCGGTTCGCC
:::::::::::::::Rv227T6.seq:::::::::::::::
CCGTTGCGCAGCGTGAGCCGATAGTTGACATCCGGCTCGGTGAAGGTGAAATCGATGGCCAGGTCGAGGTCCCATGCGCGTGGGCCATTGATGCTGATCGCCAGGA
CGTCAAAGATTTGGTCCGGCGTCAGCTGGGCGAAAAACGTGGGCGCCGGGACTTGCCCGGAGCTGCCCGGGTTCCCGTCGCGCAGCTCGGCGGCCCCGGTCAGAAA
GAAATTGCGCCAGGTCGCACACTCCGCGCCGTAGGCCAGCTGCTCCAGGGTGTCGGCATAGAGCCCGCGGGCCGCAGCGTGCTCGCTGTCGGCGAACACCGCATGG
TCGAGAAGCGTTGCCGCCCAACGGGAAATCACCTGCGTCGAAAGCTTCGCGGGCCAGCTCCAGCACTCGGTCGATGCCACCCAACGCGT
Clone Rv228
:::::::::::::::Rv228SP6.seq:::::::::::::::
ATACTCAAGCTTGCGGATGTTACCCCTGACAGCGTGAACTATGTCNAAACACACGGCACCGGAACGGTGTTGGGGGACCCCATCGAGTTCGAGTCGCTGGCGGCCA
CTTATGGCCTGGGTAAAGGCCAGGGCGAGAGCCCGTGCGCATTGGGGTCGGTCAAAACCAACATCGGCCACCTGGAGGCGGCCGCCGGTGTGGCTGGATTCATCAA
GGCGGTGCTGGCGGTGCAACGTGGGCACATTCCCCGCAACTTGCACTTCACCCGGTGGAACCCGGCCATCAACACGTCGGCGACGCGGCTGTTCGTGCCGACCGAA
AGCGCCCCGTGGCCGGCGGCTGCCGGTCCACGCAGGGCTGCGGTGTCATCGTTCGGCCTCAGCGGGACCAA
:::::::::::::::Rv228T7.seq:::::::::::::::
CCGGTAACCAGATCAGCTCGTCGACCTCACTGCCGGGGGTGAATTCCCCACCGGTGCTGCGCGCTGCCCAGTAGTGCACCTTCTTGACGCCTCGAAAAGGGGAGTC
GGTCGGGTAGGTCACCGTCAGGAGCCGCCTACCCAGGTTGGCGCGGTGACCGGTCTCCTCGAGTATCTCCCGCACCGCCCCCACCGGTGCGGTCTCGGCCGGATCC
ACTTTGCCCTTGGGCAGCGACCAGTCGTCGTAACGGGGGCGGTGAATGACAGCGATCTCGACCGGCCCTTCCGAATCGGCACTGCCGGGTCGCCAGAACACCGCAC
CGGCGGCGTACACAATCCGGCCCGCCGAGCGCCGGCGGGCGGACGANTTCTGGATCGACACCTCAACTCCTGCAGGTCAATTCGGCCAAGCTGCTCGCGGTCGTGG
ATGTGGTC
Clone Rv229
:::::::::::::::Rv229SP6.seq:::::::::::::::
ATACTCAAGCTTGATGCCGCCGAAACCGAGCGTGAGCACGCCGCCACCCACCACGCGCGGGTCGGGCGCCGGGCCCGGGCCGCCAGGCTGCTCCGCTCGGTGATGG
CACGCCACCGCGACACCACCCGGCTGCGCTACGTCGAGCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCCTCTTTCGAGGTCGAGGTCTA
TACCGATTTGCGCATCCGCAGCCGCACCCTGGTCGTCTCGTACCGTGCCCTACCTCTGCTTGTCGGGCGGGGCCA
:::::::::::::::Rv229T7.seq:::::::::::::::
TCCGTACGGCCCGGGTACGCTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCACACCTTCAGTTGCTCACCGG
AATCCAACCGGTAGAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTC
GGGGTGGTCGCGACGCGCATGGGCCACCATCCATCCACCAGGTCTGCGCGAATCACCCGC
Clone Rv22
:::::::::::::::Rv22SP6.seq:::::::::::::::
GGACACATTGCGAACATTGATGACAAAATAGAAATCATTGATGGTTTGAGTCACCAGGCCGATCAAGCCTTCGCCGAGCCAAATTCCAATCAAGAGGCCCAAGCCC
GTACCAATCAGCCCGGCAACGAGGGATTCCGTCATTATCAGCCAAAATAACTGCTCTCGGGTTACACCCAAACAGCGCAATATGGCGAAAAACGGTCGCCGTTGCA
CGACATTAAATGTCACGGTATTGTAAATTAAAAAGATACCCACCAACAAGGCAATCAAACTGAGAGCGGTTAAATTGACCGTAAAAGCGTCCGTCATCTGTTTGAC
GGTGTCCCGTTGGGTNTCCGACGTTTCCATACGCACACCGGCCGGCAGTCTTTGTTGGATGCGTGTTGCAGTGGCCTCATCTTTGATGATCA
:::::::::::::::Rv22T7.seq:::::::::::::::
GCCTGGCCCAGGTGAAGGCCGACCTCGACGCCAAAGCCGCTGATCCGGCACATGAGTCGGTGGACTGGGACTTGAAGTCGCTGCGATGGGCGTGGAACCGAGCCAA
AGATGACGTGGCGCCGTGGTGGGCCGAGAATTCCAAGGAGTGCTACTCGTCGGGGTTGGCCGATCTGGCCCAGGGCCTGGCTAATTGGAAAGCTGGCAAGAACGGG
ACCCGCAAAGGCCGGCGGGTGGGCTTCCCGCGATTCAAATCCGGGCGGCGTGATCCTGGCAGGGTGCGGTTCACCACCGGCACCATGCGCATAGAGGATGACCGGC
GCACGATCACGGTCCCGGTGATCGGGCCGCTGCGGGCCAAGGAGAACACCCGCCGGGTGCAACGCCACCTCGTGAGCGGGCGCGCGCAGATCCTGAACATGACCTT
GTCGCAGCGGTGGGG
Clone Rv230
:::::::::::::::Rv230SP6.seq:::::::::::::::
TAACTCAAGCTTCAAGTCCGCNGTCCGACCCTGTTCGACGGCTACCTGAATCAACCCGATGCCCCGCCGCGGCGTTCGACCCGACAGCTGGTACCGCACCGGCGAC
GTCGCGGTGGTCGACGGCAGTGGGATGCACCGCATCGTGGGACGCGAGTCGGTCGACTTGATCAAGTCGGGTGGATACCGGGTCGGCGCCGGTGAAATTGAAACGG
TGCTGCTCGGGCATCCGGACGTGGCGGANGCGGCAGTCGTCGGGGTGCTCGACTATTATCTAGGCCAGCGGATCGTTGCCTACGTAGTCGGCTCAGCGAATGTCGA
TGCGGACGGGCTTATCAACTTTGTTGCCCAACAACTTT
:::::::::::::::Rv230T7.seq:::::::::::::::
CCATGTCGCCCCAACATATCGTCGATGTTCGCGTCGTCCGCCTCGCGCACGTGGTCTGTCACCAGTCAACGTTAACGCCGCCGCACATGTCCTGCGGCCGGGCAAA
ACGTGAAAAACGAGCGGGCGACTGCAATGTCATGACACCGACGCCGCCGATGGGCCCAGGGTCTGGCAGATTCGATCTGTGCGGCCAGTGCCAGCAGCGTCGCCTC
GTCATACGGCCGGCCGACGAGTTGAACCGACATGGGCATGCCGTCGCCGTCGAAGTCCCACCGCACCACGGCCGCGGGCTGGCCGGTCAGATTCCANACTTGAAAG
TACTGAAGCCGCTGCACCACCAG
Clone Rv231
:::::::::::::::Rv231SP6.seq:::::::::::::::
CGAAAGCGTGAAACAGCTCGCGGCAGCCCCCGACGTGCTGCGTCGGATAGCCGGCGGGCGAAGATCAATTCCAGGCAGCTCCCGGACAATGCGGCTCTGCTGGCCC
GCAACGAAGGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCCGATCGCACAGGTTGGCCCACAACCGGCCGCTTGATGCCCGGTCGGCAAGCCCGG
CAGTTGCCAAACCCAGCGTGATCNTGCTCNGCTCTNTANTTCGGCGAAGAAGTGGCTCGCCTGATCACCTACCATCGGCCAGGATCTGCGTGTCATCACAACGCTC
GCCAAGGAGGTTGTTGTG
:::::::::::::::Rv231T7.seq:::::::::::::::
TCCGCCACGCTTCGCGCCGCCCGGCATACGGCGCGTACCGATCTCCCGCGTCATACACCGCGGGTAATCGCCGACGGTGCGGTTCGGGAGCCGAAGGTGACGACGC
TGATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGCTCAACGACGTCAATCACGTTGTCGCTTTCTACGGTCACCGACCCGGTGACCGTAGT
CGCCCGGTGCGCTCGGCCGAGAAGCTGCACCGCCACCACCGCGACACCGTCTTGCACGCGGACCCACCCCGGATCGGTTGTTGGCCAAGGTAATTGGGTCATTCCA
TTTGACGGGACGCCGACCC
Clone Rv232
:::::::::::::::Rv232SP6.seq:::::::::::::::
CATTCTTTAACAGTTGTTTTGGGCTCGGCATGGTTAGCCAACGTTCTGCGGTCCACCATATCATCTTGGTCCGGTAGCGCTCGTCCGGGGTATGCTGCCGCCGGGA
TTCTCGCTGCTATTACTCCCCCCGAAGAACCGCCACCGGTCCAGCGCGTGGGCCGNCGCGGTCCCATCACAAACTGAACCCCCAACAGGGACATGCTTATCGGTAG
GGCGCGCGCCAAGGCGGCAGCAATCGCATCACTGCGCTCTGCGCGTCACTATTAACCCACCCGGACTTCACTTCCACCACCCCGAATGGCGCCCGGTCATTGATCA
TCTGGCGCACCGCGGATAA
:::::::::::::::Rv232T7.seq:::::::::::::::
CGGTGTCCTGCAGTTGGTAGGCCTGCAGTTTGTGCATCATGCCGATGCCGCGGCCTCGTGGCCACGCATGTACAGCACCACGCCGCGCCCCTCACGGGCGAACATC
GCCAGCGCGGCGTCCAGCTGAAGCCCGCAATCGCAGCGGCGTGACCAAACACATCGCCGGTCAAGCACTCCGAATGCACCGGACCAGCACGTCGTCACCGTCGGCG
TTGGGCCCGGCGATCTCGCCGCGGACCATGCGCGACATGTTCCACGTCCTCGTANATGCTGGTGTAGCCGATGGCGCGAAACTCCCCATGACGAGTCGGAATCCGC
GCCTCGGCGACCCGCTCAATGTGCT
Clone Rv233
:::::::::::::::Rv233SP6.seq:::::::::::::::
CGGCATCTGGCGGCTGAACCTGTTCTTGGGCAACATGCCGAGGATCGCCTCTTCCACCACGCGGTCGGGGTGGCGTTGCATTACCTCACCGATGGTGCGCTTGTGC
AGGCCGCCGGGATACCCCGAGTGCCGGTAAACCATCTTGTGCTGCAGTTTGTCGCCGCTGATGGCGACCTTGTCGGCGTTGATCACGATNACNAATCACCGCCANC
GACATTGGGGGCGAACGTCGGCTCGTGCTTGCCGCGCAGCAGGCTGGCCGCCGCGACGCAAGGCGCCAACCACCACGTCCGTGGCGTCGATGACGTACCACCATCG
CGTGGTGTCACCCGCCTTGGGC
:::::::::::::::Rv233T7.seq:::::::::::::::
GCGGCAAAAATTGAAGCACTCNTGGCCACTNCCGCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGAAGGCCCGAACGTACTGCGTTTCAACACGTCG
CGTCGCCCTCCGACCGCGAACATTCTGGGATGGCAGCAACCTGTTAGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCGCGGGTAGTCGCCCCCGGGCGGCTAC
AGTCTGAAACGCGATGACCATCGATGTGTGGACGCCGCATCCGACNCAACGGTTCCTACACTGTGATATGTTCGCCTCGCTGCGCCGGTGGACGGTGGGTCTATCC
CGGA
Clone Rv234
:::::::::::::::Rv234SP6.seq:::::::::::::::
CGCGTTGAACTGAAGGGGTGCCGCCCGGCTCGAGCAGGCAAGCCATTTGTTCGATGCGGTTACCGAAGATCTCTTCGGTGACTGCCCGCCGCCGGCCAGCTCGGCT
CAGTGTCCGGCGTTGGTCGCCGCGGCGACAATCTTGGCGTCCACGGTGGTCGGGGTCATGCCCGCGAGCAGGATTGGCGAGCGGNCGGTCAGCCGGGTGAACTTCG
TCAAGAGCTGACGCTGCGGTTGGGGAGGCGAATCATGGTCGGTGCGTAGCCTCGACTAGGCCCGGG
:::::::::::::::Rv234T7.seq:::::::::::::::
TGACAACGCGGCGGCGATTACCCCGCTACCGCAGCAGCATGACGCGGTAGCGAACACCGCCGGATGCAGCGCAGGTGCGTCGATGTGCTCACGGAATCGCCCCGGC
ACCGCGATCTCGAGGATCACCAGTGCCACCCCCTGCAGCGCGACACCGACGATTCCGTACACCGCCACGCCGATCAGGCCCTGGGCCAGCTGATTGGAGCTGGCGT
ATATGGCGGCGATGGTGACGATGGTCATCGCCTCTTACATTGTGGCGGCCAGAACCACGGCGTTGGGGCGGCGGTCGATGAACACTAGGCGACCANATCCCCGGGG
TCAACAGGTTGACCATCC
Clone Rv235
:::::::::::::::Rv235SP6.seq:::::::::::::::
CGCGGACATCCCGAACGAGGACACGCGACCGCTTCGGTGTGTGATCTATCAGGGCTCGCACCACGCGCAACCGCTTCCGGCTACCTAGACGCGGT
:::::::::::::::Rv235T7.seq:::::::::::::::
GCATGCGGGTGATGCCGTTCTCAGTGCGCAACAGCGTTCGACGCGGCATACCCAGCCGCACATGCCGTGCACGCCCGAGCCGGGGCGGGAATCT
Clone Rv237
:::::::::::::::Rv237SP6.seq:::::::::::::::
CTCAAGCTTCAGNCCNTCTAAGCGGTCTGCGCGGCGATCGCAAAGATCGCCCTTTGCCGGCGTTGGGGGCTTCTGCTCGGGGGTGTTGTACACCTTCTCGAACACC
TCGGCACCGACACCACCACCGTCGGCTTGAACACCGCCAACATCGGCAGCANATCTTGATGTCCTGGTGAATCCACGGTGACTTTGGAGTGGAAGGCGGCCATACT
GATCGCGCGCGCCACCACATGAGCTAGCGGCAGGAAAACCAGCAGCCGCTCACCCTTGCGCAGCAGCGTCGGGTGATATGCCTGGCGCCC
:::::::::::::::Rv237T7.seq:::::::::::::::
AGTCGAANGTCAGTCCGGTCTCCTCTCCGACTACGGCCAAGAACTGGGGCGACGGTGTCAGTGCAGAACAGCGGAAACTGGTGGCGCCCTAGGCGAGCGAACGCTC
ACAAACGGCGGTGACCGCTTCTGGTCGTGCACCATCGAGCCGTGCCCAGCCCGGCCGCGTGCCGTCAGCCGCATCCACTGGATGCCCTTCTCGGCGGTTTCAATCA
NGTACAGGCGACGTTCGCCACCATCGTGCCGGGGCACGTTAGCGAGAAACGCCGACTTCACCGATTGCCTCGGTGATGxxxxx
Clone Rv23
:::::::::::::::Rv23T7.seq:::::::::::::::
AGCTTCGCGGCGTGGCGATCGCGGTTCAAGGCGCGCTCTTCGAGCACAACGAGCGAAGACAGCTCGGCGACGGAGCCTTTATCGACATCCGTTCGGGCTGGCTGAC
CGGCGGCGAAGAACTGCTGGACGCGTTGTTGTCGACGGTGCCGTGGCGAGCCGAGCGCCGTCAGATGTNCGACCGGGTGGTCGATGTGCCGCGGCTGGTGAGTTTT
CACGAGGTGACCATCGAAGATCCGCCGCATCCGCAGCTGGCGCGGATGCGCCGGCGGCTCAACGACATCTACGGCGGCGAACTGGGTGAGCCCTTCACCACCGCCG
GGCTGTGCTACTACCGCGACGGCTCTGACAGGGTCGCCTGGCATGGCGACACCATTGGTCGCGGCAGCACTGAGGACACTATGGTGGCGATCGTCAGCCTCGGCGC
CACCCGCGTCTTCGCGCTGCGGCCGCGTGG
Clone Rv240
:::::::::::::::Rv240SP6.seq:::::::::::::::
AGCTTCAGCTGATACTCGACCAGCCCCACTCGGGCCAATACGTGAATGTCTAGCATCTTCACCCGTTCACGGGCTANTCGAGTAGTAGACATTGATTAGCCTGAAC
GTACCTCCGACGCCAGCTGACGAACGGGTATGCGGATGGATTTCGTGGTGTCGCGCCCGAAGGTCAATTCGTTACGGATGTATCTCGGGGCCGGATCGGGGCCGAT
GTTGGCGGCCGCGGCGGCCTGGGACGGACTATCCGACGAACTGGCGGTGGCGGCGTCGTGGTTTGGGTCGGTGACCTCGGGGCCTGGCGGATGCGGCGTGCGCGGC
CCGCGGCGGTTGCGATGGCNCGCGCGGT
:::::::::::::::Rv240T7.seq:::::::::::::::
CTGGTCATGGACGTTGCTCCGGTAGTGGCTCACTGCCGATCCTCCTCGTTGAGAGTGCCACCTCAGGGTTGGGTAGGGTTGGGTACTCGAAACCAAGTTACCCACC
AGTAACACCGTCAAAATATATCCGTTGCATAGGTCAATGCAAGTTGATGTGAGCTACATTGCACCAACTAACTAACCAACCGGTTGGGTTAGCGGTGATCCTGGCC
GTGTCGGTCCTCTCACCTGCGGTGATAGCGATCAAATGAAGAATATGCGGAGTCTAGGGCGGCAGCGCCTGGCANCGTAGATCATCGGCTCACGCGGATGCGGCCT
CTTGGTACGGACATGCGCGCG
Clone Rv241
:::::::::::::::Rv241SP6.seq:::::::::::::::
CTCGTGAGTAGCACCCCTGTAATTTGGGATCGGCAAAAAGGCGAATCACCGCGTGGCCACGACACGCCGGGAGGGACNATCTCGGGCGGCTAGGGCTTCTCGCGGG
AAGGCCCGAACGTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGG
:::::::::::::::Rv241T7.seq:::::::::::::::
GGATCAACTACCGGCCAACGGTGATTCTTGGGCGCCGCTGACGCGCAACGACCCAGCGACACATTCAGCAGATTGGCCAGCGCGTGCCGGGCCACGATGTTGGTGC
TCGGCGGCTACTCCCATGGTGCGGCNCGTGATCGACATCGTCACCGCCGCACCACTGCCGGCCTCGGGTTCACGCAGCCGTTGCCGCCCGCAGCGGACGATCACAT
C
Clone Rv243
:::::::::::::::Rv243SP6.seq:::::::::::::::
AGGACCGTCAGCACGGCGACGTGCTACTCGCCGAGCAGTGGGAATCGCTCTGCAGCAAACCATTACTCTGCGCGACGTTCGAGATGACCTTCTGAATGGACGGATC
TACCTGCCGCGCGACGACCTGGACCGCGTATGCGTCCGCCTCCGCCTGGACGACACCGGGGCACTCTATGACCCCGACGGACGGCTCGCGGTACTGCTGCGGTTCA
CCGCCGAGCCCCGCACGGTACGCGTCGGGACTGCGCTGAGTCCANCCTCGACGCCGTAGCGCTGCTGCTGTGCGGCCATGTCTGGCATCTACCGCCGTCGCTCCCT
TGA
:::::::::::::::Rv243T7.seq:::::::::::::::
CGACTCTGTTGGCCACTGCGGGTCGATCTTGCGGCCGCCCCGGTCGTGGAACGCCCAGGTCACCCGGCGGCGCACCGCGGTCAGCGCGTCGTTGGCCAGCGTGGTC
ACATGGAAGTGGTCGACGACGAGCTTGGCGTTGGGCAGCAGCCCGGGCGTGCGGATCGCCGAGGCGTATGCAGCGGCGGGGTCGATGGCCACCGTACTGGATGCTC
TCCCGGAACTGCGGTGTGCGCGCTTGCAGCCATGCCAGCACCGCCGCGCCGCCGCGGCCTTCATGCTGCCCATAAACCCTGATACCGGCCAGGTCGACNAACCNGT
ATCCCACGGTCAACCC
Clone Rv244
:::::::::::::::Rv244SP6.seq:::::::::::::::
CACACGGACGGCGGTGCGGACGCAGCTGACGCGCATGGTGGTCAGCATCGCGGCCGGTCTGCTGTTGTATGCCTACTTCGCGCCGCGCAAATGCTGGTGGGCGGCG
GTGGTGGCGCTCGCATGGCTGGGCTGGGTGCTGACCCAACTCTCGAACCACACCCGGTGGGTGGGCTGGGCTATGGCCTGCCATATCGGCCTGGTGTTCTACN
:::::::::::::::Rv244T7.seq:::::::::::::::
CCGATATCCGAGCCGATAGCTGGCGGGCTCGGGTGGTNGCCAGCGGCGCTGCGACGAAAGTGTGACCGTCATGAAACAGACACCACCGGCGGCCGTCGGCCGTCGT
CACCTGCTCGAGATCTCAGCATCCGCAGCCGGTGTGATCGCGCTTTCGGCGTGTAGTGGGTCGCCGCCCGAGCCCGGCAAACGCCGGCCCGACACAACCCCGGAAC
AGGAAGTCCGGTCACCGCGCC
Clone Rv245
:::::::::::::::Rv245SP6.seq:::::::::::::::
GCTTCAGGACAAATTGNATCCCTATGCACCCGTTGTCACGCCGATGAGTGAAGACTGCACCGCAATCGCCGGAATCCGGCAAACCCTGCACAAGCGAAATCAACCG
GAGGCTGACAAGGCAACGTCGGTGATCCGTACCGCCTGGTTGGACAAACGGCAGAAGGCGCCTCGTCCGGTCCATCTACGCCGAGCACACTGGTGATAGCGCCATC
GGCATCGGTGCGGCCACGGTGGAGACGAACGTCCGCNGGCGTCTGGGTCAGTAACCCGCCGACCAGTTCTCGGGCAAGCTGGTCAACATCGGGCGCCACGTCTCCA
AC
:::::::::::::::Rv245T7.seq:::::::::::::::
GTTTGGCGGCCTTATTGCACTGAGGTCGTCAATTGACCCACAGCGGAAATGCCGACTATTCGCAGGCCTCCTTGGCCTTGGCTGCCGGAGATGGGCTCCGCGGGAA
CCGCATGCAGGTATATGACCTCGGTTTCTCGGGTGCTACCGCGTGCCTTGTCGAGGATGAACTCGGCGTTGGAATTGTCCAGCCGGCCCAATTCATCGAGCGCAGA
TTCGTACACATGGCCGGCGGCGACATACCTTCACCGTGGATCTGCTCCACACGGACCGCCCTGTCGGGATCTGCTCACGGGTAAAGGAATTA
Clone Rv246
:::::::::::::::Rv246SP6.seq:::::::::::::::
GCGCACTCCTCCTTATCGCTCCGCTCTGCATCGTCGCGGCGCGGTCAGGTGCAAACGCCTTCGGGGGTGGGGGTCCTGCGGAGCACACCGGATACGGAGCGCAACG
CGTCGCGTTGTGCGGGCAAACAAGTGTGCAGGNNCCAATGCCATGTCCAGCAGCTTATCAGTGTCGAACGTGCGAACGTCGCGCCTTCGCCGGTGCCTGAATCTCT
ACAAG
:::::::::::::::Rv246T7.seq:::::::::::::::
CGCTGAAAGCCACCATTCGCCGGGTCGGCGCCGGGCTCGGGCCGCCAGGCTGCTCCGCTCGGTGATGGCACGCCACCGCGACACCACCCGGCTGCGCTACGTCGAG
CCATACCGGGCGGAGCTACATCGGCTCGGCCGCCTAGTGTTCGGGNCCTCTTTCGAGGTCGAGGTCGA
Clone Rv247
:::::::::::::::Rv247SP6.seq:::::::::::::::
TGTAATTTGGGATGGGCAAAAAGCAAANCACCGCGTGGCCACAAACGCGGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGAAGCCCGAAACGTACGGCG
TTTCAACACGTCGCGTCGCCTCCGACGCCGAAATTCGGG
:::::::::::::::Rv247T7.seq:::::::::::::::
CTTGGGCAACATGCTGAGGATCGCCTTTTCACCACGCGGTCGGGGTGGCGTTGCATTAGCTCACCGATGGTGCGCTTGTTGCAGGCCGCCGGGATACCCGAGTGCC
GGTAAACCATCTTGTGCTGCAGTTTGTCCCGCTGATGGCGACCTTGTCGCGTTGATCACGATGACGAAGTCACCGCCATCGACATTGGGGGCGAACTCGGCTTGTG
CTTG
Clone Rv249
:::::::::::::::Rv249SP6.seq:::::::::::::::
GCATGCTTCATTATCTAATCTCCAGCCGTGGTTTAATCAGACGATCGAAAATTCATGCAGACGGTCCCAAATAGAAAGACATTCTCCAGGCACCAGTTGAAGAGGT
TGATCAATGGTCTGTTCAAAAACAAGTTCTCATCCGGATTGAACTTTACCAACTTCATCCGTTTCATGTACAACATTTTTAGAANCATGCTTC
Clone Rv24
:::::::::::::::Rv24SP6.seq:::::::::::::::
ATACTCAAGCCTGATGCCGCCGAAACCGAGCGTGAGCACGCCGCCAGCCACCACGCGCGGGTCGGGCGCCGGGCCCGGGCCGCCAGGCTGCTCCGCTCGGTGATGG
CACGCCACCGCGACACCACCCGGCTGCGCTACGTCTATCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCCATTGTTCNGGCCCTCTTTCGAGGTCGAGGTCTA
TACCGATTTGCGCATCCG
:::::::::::::::Rv24T7.seq:::::::::::::::
TCCGTACTGGTCGGGTACGCTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCACACCTTCAGTTGCTCACCGG
AATCCAACCGGTAGAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAACCTCGGGTCCG
Clone Rv251
:::::::::::::::Rv251SP6.seq:::::::::::::::
GTTCTCGCACGATTTCGGATTAGCGGGATGGTCTCAATTGGGTATGCGGGGAAGGCGCTGACATTCGCCGCGATTAGCTGTTTGATGGACCGGGGGTGATTTTTGA
TCACGGAAATGGGTGTTTATNCAGGTCGCACGCTTTCATCCGGGGCGGAACG
:::::::::::::::Rv251T7.seq:::::::::::::::
GGGTGTGCCTGCTGTGTATGCACGGCATACGGACATCCTTCCCCTGAAGACCCGCGGTCGAACAGCCACGTGTCCATCATCANGGGGTCAACCCCGGCCAAGGGCG
ACGGCACGCCAAGTTCGCCGACCGTTAACCTAGTGCTGTTAGCTTCATTTGCTGCGAGCAAAACAGCTGGTCGGNCGTTAGGAATGAATTGAAACTCAACCGATTT
GGTGCCGCCGTAGGTGTCCTGGCTG
Clone Rv252
:::::::::::::::Rv252T7.seq:::::::::::::::
ACTACCCGGCCAACGGTGATNTCTTGGCCGCCGCTGACNGCGCGAACGACGCCAGCGACCACATTCAGCAGATGGCCAGCGCGTGCCGGGCCACGANGTTGGTGCT
CGGCGGCTACTCCCANGGTGCGGNCGTGATCGACATCNTCACCGCCGCACCACTGCCCGGCCTCGGGTTCACCAGCCGTTGCCGCCCGCAGCGGACGATCACATCG
CTTTTATTTNNTNTTCNGGAATCCCTCGGGCCGCGCTGGCGGGCTGATGA
Clone Rv253
:::::::::::::::Rv253SP6.seq:::::::::::::::
ACGTCGGGANACTGTTCGCGTTCATCCTCGTCTCGGCGGATTGGTCTGCTGCGCCGGACCGACCGATCTTCAGCGGGGGGTCACGCTCCGTGGGGTGCCGTTACTT
CCGATCGCCCAGTGTGCGCGTGCTGTGGCTGATGCTGAACCTCACCGCGTTGANTTGGATCGGTTCGGGATCTGGCTGGTGGCCGGAACGCNATTTATGTCGCTAC
GGGCGCCGGC
:::::::::::::::Rv253T7.seq:::::::::::::::
GCTCAAAGGCACTACTGGCACCAAGGCCCACACGTCACCTGTGACTCCTGCGCCGACCCGCCCGAGGTCTGGCCGTTACACCGAACGGGCGAGCCGGGAGTTGGTA
CCATCGAACAAGACAAGGTGCATGGGCGGAGTTGTTCCGCCACTTCGTCGATGACGGGTC
Clone Rv254
:::::::::::::::Rv254SP6.seq:::::::::::::::
CGATACCGGCTGCTTACCGAGACATCCACCATGCCACCCGAATCACCGCACGCGCCGAAATCGCACAACAGCTTGACGCCTTGCAGGTTCCGCGATTGGAATTGCC
GACGGTCTCTGACGGCGTCGACCTTGGCAGCCTCTACGAGCTCTCGGAATCACTTGCCCAGCAGGGGGTTCGATGAGTGTCACACCGAAGACCTCGATATGGGCGC
AATCCTGGCCGACACATCCAACCGGGTGGTTGTGTGCTGCGGCGCCGGTGGGGTCNGCAANACACTACCGCGGCCGCGCTGGCGTTGCGCGCGGCCGAATATGGCC
GCACTGTGGTCG
:::::::::::::::Rv254T7.seq:::::::::::::::
CGTCGTCGTCGTGGTATGCGATAGCCATCCCGTCGGGCTACTCGCCATCACCGATCAGCTTCGCCCCGAAGCCGCCGCGGCGATTTCCGCTGCGACCAAACTGACC
GGGGCCAAACCGGTATTGCTTACCGGCGACAACCGGGCCACCGCCGATCGGCTCGGTGTACANGTTGGCATCGACGACGTACGGGCCGGGCTACTGCCGACGACAA
NGTCGCAGCCGTGCNGCNGCTGCAAGCTGGAGGTGCCAGATTGACCGTGGTCGGTGACGGTATCAACGACCTCCGGCCTTAGCGGCCGCGCATGTCGCATCGCCAT
GGGCAGCGCCCGAC
Clone Rv255
:::::::::::::::Rv255SP6.seq:::::::::::::::
GCACGCAATCGAAGTCACCCAAACCGGGCGGGCCAGGCGTCTNACGCCACGTCNACCAGCCGCAACCTCAACCCGGCCACGGCGAGCTCCTGATCAAGGCCGAGGC
CATCGGTGTCTACTTCATCGACACCTACTTCCGCTCCGGGCAATATCCGCGCGAACTCCCGTTCGTCATCTGCTCCGAAGTATGCGGCACGGTGGANGCCGTCGGC
CAGGGGTTAC
:::::::::::::::Rv255T7.seq:::::::::::::::
TCGACTGTGTGGCCACAGATCACGCCCCGCATGCCGAGCACGAGAAATGCGTCGAATTCGCCGCGGGCCGGCCGGCATGCTCGGGTTGCAGACGGCATTGTCGGTG
GTGGTGCATACAATGGTGGCGCCGGCTTGTTGANTTNGGCGCGATATCGCGCGGGTGATGAGTGANAACCGGCGTGCA
Clone Rv257
:::::::::::::::Rv257SP6.seq:::::::::::::::
GAACCTGACACCCTGGTCACGGGTGAGCACGGACTTGATTTCTTCNCTATTGGTCGGCGCTGTTGAGCACACCACGCCGCTGACGGCCGTCGCGTCCTCGCTGTGC
TCGGTCTGGTGGAGCGCGCTGCCCGCGGCCNAACATCNTAAATCAAGCGTATTCGTCAACAGATATCATCAATGTCGGCGCTGGACTATTCAAATCATCGATATAC
TGGTGACCTGGTCCTTCGCCATCGATCAATGGCGATAGTCACGCAAATCGTCACGGACATCGTCGGCGTCCCAGCTGGCCCGTGCCAACAGATGCTGCAACCCATC
GGGGTGGTATCACCGCGGTGCTCGGCGATGGTCCACAATTCTTGCGGTCCAAGCCCNAAACATCCCGGGCATGAATTCACCGGCATGCGCN
:::::::::::::::Rv257T.seq:::::::::::::::
CTATCGTACCCGCGCCGGTCACCTTCTGGATATCGCCGGCCTGGTCAAGGGGGCGTCCGAGGGAGCCGGGCTGGGTNACAAGTTCCTGGCTCATATCCGCGAATGC
GACGCCATTTGTCAGGTGGTGCGGGTGTTCGTCGACGACGACGTGACTCATGTCACCGGACGGGTCGATCCCCAGTCCGACATTGAGGTCGTCGAGACCGAGCTGA
TCCTGGCAGATCTGCAAACCCTGGAGCGGGCCACGGGCCGGCTGGAGAATGAAGCGCGCACCAACAAGGCGCGCAAGCCGGTCTACGAAGCGGCACTGCGTGCCCA
GCANGTGCTCGACGCCGGGCAAGACGCTGTTCGCCGCGGGGGTGGATGCCGCCGCGTTGCGCGACTGAAACTGCTGACCACCAAGCCCTTCCTGT
Clone Rv258
:::::::::::::::Rv258SP6.seq:::::::::::::::
TACTCAAGCTTCAGGCCGCCACGTCCGCCGTCCGTCGGCGACGTGACCTCGAGCGCCGAGTTCGACTCGACATCGCCGCCGGCGCATGCCGACATGAACGCGGCAC
TCACCGCAAGCCCGTCGGACGTCAGGTCGATCGACTCCGCTTCAAGCACCGGATCGTCCGGGCAACTCGCGGCCTCGGCCTGTGCGAACGGCACACCCGTCGTGGC
GGCNCCCCGCGCGGAACTGGGCTCATCACGGTCGTTGCGAGCCGGTCGCGTCACCGCGTACCGACGCCGTC
:::::::::::::::Rv258T7.seq:::::::::::::::
CCGACATCGAGTGGGCTCGCAGTGACTTGGCGACCTCCAAGCCACCGGTACCCGCCGCGCGGCAAGCCAAGGACGACGACGGCCTTGCCGGATAGCTGCGCCAGGC
GTTGCGCCAACTGGCGTCCAGCGTCGCCACGATCGTCAAAGAGCTTCATCTGCCGAGTGTGTCGCCATCTCATGGCTCCAAATATGGAATTAGGTCCCTGGGCCGA
CTGACGACAGTCCCTCAGCGACCGGATTGCGCATCCCGCCTTGTACGCTACTCCGCAAATCCCGGGCTTGCGTCCGCGGAAGCGAACTCGGCGGCGCTACGTGGTG
GTTCACTTCGGCCGTGCGCACTCGGATCGACGGGCCGATGGTGGCCGGGCCCGCGCGCTTCTTGGTCATCCGATTGAGT
Clone Rv259
:::::::::::::::Rv259SP6.seq:::::::::::::::
ATACTCAAGCTTGTCGCGGTAAACCGCACGCAGGGCGGTGGGTGCGGTGTCAAAGACACCCACACTTCTTTGCGGTTCGGTGATCTCGACACCGGCCGCGAGCCGA
CCACCATGCGCGCGTAGATCGGCGATCAGCGCGTCGGCTATCGCCTGGGTGCCGCCCACCGGAATCGGCCAGCCGACCGAATGGGCCAGCGTTGCCAGCATCAGTC
CGGCGCCGGCCGACACCAGTGACGGCAACGGTGAAATCGCGTGGGCGGCAACGCCGGTGAACAACGCGCGGGCATCCTCGCCCGCCAACGACCGCCACCGAGGGTG
CCTGGGCCATCATCCGCAGCCCGA
:::::::::::::::Rv259T7.seq:::::::::::::::
TGGACTCATAACGATCGGGTCAGCGACGCGCCAACACGAACGGCCGGACGAGTGGGCCAGGGTCGCGCCTCCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAG
GCTCCGGTGCGGCGTTGGGCGCCGTGCTCGTCCCAGCGTCCGGTCCCGGGTCGCCGGCGACGCTTGTTTCCTCCATACTCGCCCCCTAATCTCGAGGCAGCCCGTA
CCCGCAGGCAACCTCCCAAAAATGCAATCCCCCAAAATGCAATGCGTCGAGCTATTTCTCACACCGACCGCTAGTTGCGGATCAGAAATCCGTTGGGCGCGGAAGT
CCAGCCGAATTTGTTCTCCCGCTCCGCATCATGCTTGTAATCGTTTGGAAATCATCCTCATATGCCTCGATCGCTTCATAGGTCAAGGCCAAACCCGGCAGGATGG
GTGGCC
Clone Rv25
:::::::::::::::Rv25SP6.seq:::::::::::::::
CTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTG
ACACTATAGAATACTCAAGCTTAGTGGTTGCGCACGTAAATTCGTCAGGTGACCGATCCCCTGCTGTCTCACTCGCCTCACAGCGACCACCACGGCTGGCGCTCAA
GGCGGGCACGTGCGGAGCAGATGAGGAATGTGCGACGTCTTGATGCAGCCTGTCAGAACACCGAGACCCTCGACGAACTTACGATCGAAACCGCTTAGGCCAACCG
GTGACGGGGGTGTCTTTCCGCGGCTAGGGCGCCTTATCGTCCGAAGGCCGTGGGTGGTGATCGCCTTCTGGGTCGCGCTTGCGGGTCTGCTTGCGCCGACGGTGCC
GTCCCTGGACCGATCTCCCAGCGGCATCCAGTGGCGATTCTGCCATCGG
:::::::::::::::Rv25T7.seq:::::::::::::::
CAGGCATGCAAGCTTGCGATGTATCAACACGCCGTTGCGCAGCGTGAGCCGATAGTTGACATCCGGCTCGGTGAAGGTGAAATCGATGGCCAGGTCGAGGTCCCAT
GCGCGTGGGCCATTGATGCTGATCGCCAGGACGTCAAAGATTTGGTCCGGCGTCAGCTGGGCGAAAAACGTGGGCGCCGGGACTTGCCCGGAGCTGCCCGGGTTCC
CGTCGCGCAGCTCGGCGGCCCCGGTCAGAAAGAAATTGCGCCAGGTCGCACACTCCGCGCCGTAGGCCAGCTGCTCCACGGTGTCGGCATATAGCCCGCCGGCCGC
AGCGTGCTCGCTGTCGGCGAACACCGCATGGTCGAGAAGCGTTGCCGCCCAACGGAAATCACTGCGTCAAAGCTTCGCCGGGCCACTCCAGCACTCCGTC
Clone Rv260
:::::::::::::::Rv260SP6.seq:::::::::::::::
ATACTCAAGCTTGACCGACGCTGATCGCACCGCACGCGGGAACCTCAAGGGCACTACTGGCACAAGGGCCCACACGTCAACCTGTTAACTCCTGCGCCGACCCCGG
CCGAAGTCCTTGGCGTTAACACCGAACGGGCCAACCCGGGAATTTGGGTTCCATCAAAACAAATAGCAGGTGCCTGGGCGGAGTGTTC
:::::::::::::::Rv260T7.seq:::::::::::::::
GTCGTCGTGTGCTGGGGCGTCCGTATCAGCACGCCCACGAAATGGGGCACAAGAAGGATTCCTGGAACGGTGGCTGTCCAAGATCACCCTCGCCCAAAACTGCTAC
GGGCACTTCTACATCGAGCACAACCGTGGCCATCACGTCCGCGGTGTCCACACCGGGAGG
Clone Rv261
:::::::::::::::Rv261SP6w.seq:::::::::::::::
ATATGCCTTGCTGAGCTTTTCGGATCGCAGCGAGTCGTACCCGCGCCGGTCACCTTCGTGGATATCGCCGGCCTGGTCAAGGGGGCGTCCGAGGGAGCCGGGCTGG
GTAACAAGTTCCTGGCTCATATCCGCGAATGCGACGCCATTTGTCAGGTGGTGCGGGTGTTCGTCGACAACGACGTGACTCATGTCACCGGACGGGTCGATCCCCA
GTCCGACATTGAGGTCGTCGAGACCGAGCTGATCCTGGCAGATCTGCAAGCCCTGGAGCGGGCCACGGGGCGGCTNGAA
:::::::::::::::Rv261T7.seq:::::::::::::::
GACACCCTGGTCACGGGTGAGCAGGACTCGATTTCTTCGCTATTGGTCGGCGCTGTTGAGGCACAGCACGCCGCTGAGGCCGTCGCGTCCTCGCTGTGCTCGGTCT
GGTGGAGCGCGCTGCCCGCGGCCGAACATCGTAAATCAAGCGTATTCGTCAACAGATATCATCAATGTCGGCGCTGGACTATTCAAATCATCGATATACTGGTGAC
CTGGTCCTTCGGCCATCGATCAATGGCGATAGTCACGCAGATCGTCACGGCATCGTCTGCGTCCCAGCTGGCCCGTGCCAACAGATGCTGCACCCCATCGGGGTGG
TATCNCCGCGGTGCTCGGCGATGGTCCAACAATTCTTGCGGTCCAAGCCCGAAACCATCCGGCCATGAGTTCACCGGCATGGCGCAACGGCTGGTGCCGGGCAAAA
CGCGGCGCGATCGAATTC
Clone Rv262
:::::::::::::::Rv262SP6.seq:::::::::::::::
TGTAGAAGGTGGGTCCCGTCCAACTTCGCGGCGGCGGCGCGATATGCCTTGCTGGTCTTGCTCATTTGATATCCAATCTATGGGTCGTGGTTACTCAACGGGCCGA
AGCTGGCCCTCCCACGGGTAGGGTCCTATTCGACGGTGATGTCC
:::::::::::::::Rv262T7.seq:::::::::::::::
CCCGAATCCGGTGGCCGGCAGGGGGCCTGGCGACGTGGACACCTTCTAACTTGTCTTTACCGGTCACTGTTGCACCCCAACACCTTTAACGACGTGGACGGACGTT
ACATCGGATTCGACGGTGTCATCCACAGCGTTGCCATTGGGCACACCCACTACGCCAATTTCTCCGACTGGGACACCTACCGCAGCCTCGCCCCACTGCAGGGACT
GTTGTTCCCGCAACGGGCCATCGACATGATCCAGTCGTTGGTGACCGACGCGGAGCAGACTGGTGCGTATCCGCGTTGGGCGCTGGCGAAATTCCGCCACCGGCAT
GAT
Clone Rv263
:::::::::::::::Rv263SP6.seq:::::::::::::::
TTGAGATGCTGGTCGGGATGCCGATGGTTGGAACATGGTCCCCTGGCGTCGAATACGCGCGAGCGCATGAGCTCACCGGTTCGGAACAACGTATCGAAGAACTCGC
ACTGCTGGCAGATGGTATCTCCGATGTGGTTGTAATTTGTATCCCAACTCTAACTGTGCTATCGGATCTGCGTGAATA
:::::::::::::::Rv263T7.seq:::::::::::::::
CGTAATCACGATCCCGCTGAGACACTTGACCTTACGGCCGAAGTGACTTCGCTGCTGCTATGCCGACACCCGATTTCCATACGCTGCTGTACACGACGGCCGGGCC
GGTGGCCTCCATCACGCTCAACCGCCCGGAACAGCTCAACACCATCGTCCCGCCCATGCCCGACGAGATCGAGGCCGCTATCGGGTTGGTCGAACGCGACCAGGAC
ATCAAGGTCATCNTNCTGCGCGGTGGCGGGCGCGCCTTCTCCGGCGG
Clone Rv264
:::::::::::::::Rv264SP6.seq:::::::::::::::
CAAGCTTAAGCTGGTTCCGGCCACTCCATGAGCCGTAGTGCAATGGTTCGTGCACGGCGAGGCCGAACTTGCCATAAACATCCCTGACGAAAGTCTCCGGCAAGCC
GATTGCTTCTTCGGGCCGCTTCTTGTGGATTGTCCGATAACCCGGTCCCTCATGCTGGAAGTTGTGCGCACTCTTTCCTTCCGCGATGTGGGCTAACGACTCGTCA
TTGAGCAAGAAGTACGTGCACAGGCATCGTCCGCCGGGCTTCAGCACGCGGGAGATCTCGTCCAGATAGTGCTCCACGTCCGGNGGGAAACATGTGGGTGAACACC
GAGGTNAGAAACACCNCATCCAACGACGCATCCGGGATATGGAAAGCGAAA
:::::::::::::::Rv264T7.seq:::::::::::::::
TATGGTCTTCGTCGACCAGTACGTCGTAGGCGCCATGAGCCAGCGACTGAAGCCGCGCCATGCCTGCACGGCCCGCTCATCCAGCGAGGCGGCCATCTCCCGCAGA
TAGCCTGCCGCCTCGGCGCGCACGCTGTCCGGATCGCGTCCGAGCTCGTCGGCCAGCGCACGCAGCCGCTCGTCATACCATCGGGCATCCAGCAGTTGGGTAACCT
CAACGGGGTCGGTCGCTAGCGGCGTCATTGATTCAGCAACAATACCGATGCGCTGCAGCAACTTTCGCAGTCCGATGCGGCCCACCTCCCGTGCAGTCACTGGCTA
GCCCCCGTCATGCCGGTTGTGTCGATGGCACGGCAGCGGGCTCGTAAACCTGCGGTCTCAGCTCGCTGG
Clone Rv265
:::::::::::::::Rv265SP6.seq:::::::::::::::
GCTTAGCGGTCTTGCTCGAACCGACATTGCGTGCCACTCATGAGCGGGTGGCGGTCGCGGTGCTTACACATCT
:::::::::::::::Rv265T7.seq:::::::::::::::
GTATCTGGCGCCTCTCGAATATCCTTGAACGTCCCGCGGTGCCACCCAGATAGATCGCAGCGCCCTGCAATGGAGTTCCCTTTATGGCCTCTCTAGCCTCCCGCTT
GATCGGCTCGACCCGAGAGATGCCCTCGGGCGTTGCGGGATCTCCCTCCA
Clone Rv266
:::::::::::::::Rv266SP6.seq:::::::::::::::
CTTCACGCCGATCCGCGACCGCGAACGCGACGGTGACGGTGGCGACAAGGTTCGGTTGGTCGCCGCGGCGCTGGGCGATATCAGCTCACCCGGTTTCGAAGGTGTT
CGGCGACCGGACGGTGCTGCAGACATTCTTGAGCGTCCTCGACCGGCCCGATTCGGCCTTCAACATCGTGACGCCGTATTTCGGCGGTACCGCTCGGCGCCGAGTC
GAAGGCGGCCTGAGCTAAAGCCGGGCATTGCGCGAGTGGTAAACAAGTTCGGTGACTTCGGTTGACCGACTCGACGGGCTCGATCTGGGCGCGCTGGACCGGTATC
TGCGTTCGCTGGGGATCGGGCCNACCGCNANTTGCGTTGCGANCTGATTCCGGTGGAGCTCCAATCTGACTTCCGG
:::::::::::::::Rv266T7.seq:::::::::::::::
GCAGCTACCGACCCTAGCGACGAGTGTGTTCGCAGCGTCGAATGTGAACGTTCGGCGTGATTCGGCGCGCGGGTTCCCGCTCTCAGCGCACGTTCGGCGCCGAGGN
GGCTAGTCCCTGGTTAAGCAATGTCTCGGTCGCCGCCAGCAGCGCGCATGTCGCCAACCCGTCNACCGCGTTGCGCATGTCCGGTACCGACGGAAACGACGGCGCG
ATCCGGATGTTCTTGTCGTCCGGATCCTTTCGATACGGGAACGACCCCCCGCCTCGGTCACCGCGATACCAACGTCCTTAGCCAANGCTACNGTCCGGCGCGCGGT
CCCGGGCAACACGTCGAAGCTGATGAANTAACCACCCTTGGGCTCGGTCCAAGANGCGATCTTGGACTCCTTAACCGCTGATNCAA
Clone Rv267
:::::::::::::::Rv267IS1081N60.seq:::::::::::::::
TCCCCATCGGCGCCGGACCGTTTGAAAGTCCAAGCACGGGTGGGATGGAATCGACGACAGTTGAGCGCCGTCGGTGGCCGTGGTCAGCAGCTGTTCGCGAACGCAC
CAGGTCACATCCCTTCGACATCTCACCGACGTGGCACGGGCGACATCAACAGGAAGATTGACGAATCCCTCGCAGGCGCGGCACGTCCGCAGGCCAACGCCAACTA
CGGGGCCACCAGCGATCCTCCGCTCACGCACCAGCCCAAGCCAGGCTCANCCACCCAAGTCGGCCCGCGCTCTCCCTGGCCCCCTGGTCTCCGGGGCCTTGTTAAA
CAACTACCGGAAGTCCACCAATCCTCGCTGCATCTCGACACCGTCCGCCTCACTCCCTTCCTCCCGCCCCTCTCCACACNACACACCTCTTGCATTAAGGTCACGG
AGCGGTCACTTTTCGTCGGACGAAATTCGCAATCCGGCCGCTCGCCGCCAGAGAT
:::::::::::::::Rv267SP6.seq:::::::::::::::
CGGAAAGTGGATACTCCCAGCAGGTAGCAGGTCGCCACCACGCTGGTCAGTGCGCGTTCAGCTCGCTTGCGGCGCTGCAGCAGCCAGTCCGGGAAATAGCTGCCCT
GGCGCAGCTTGGGGATCGCGACGTCGATGGTTGCGGCACGGGTGTCGCAAATCACGGTGGCGGTAGCCGTTGCGCTGATTGGACCGCTCATCGCTGCGTTCGCGGT
AGCCCGCCCCGCACAGGGCGTCGGCTTCAGCCCCCATCAGGCGGCGA
:::::::::::::::Rv267T7.seq:::::::::::::::
GGCCGAGTCCAGCACTTCGCACTATGTGCAGACCAAANACCCGGTGGTCGCCGCGCTGCGGCAGCGGCTGGCAACGGCGCCGGTGATCACCGAGTGGTGCGNAGTT
GCCGACCGGCAGTTCGCCGCGGGCTTACTACGAGAAGGGCCTGCGCGACGTCATCAGGTATCACGTGTCGATGACGTCGAGCGTTAACTTCCCCGACCAGACGGCG
ACCTCGCCGATGGACCCCGCGTTGTACCTGGTGTGGGCGCAAGCTAACGCCGCCGCANGCTATCGGTACTCGGTCGAAGCGCAGCCGGGGTCGCAAGCGCTAGCGG
GCAAGGTCGCGACGATCTCGGTCACCTGGACCAACTACGGCGCTGCTGCCGCCACCGAATAGTGNGTGCCCGGCTACCGGCTGGTGGATTCCACGGGACATGTGGT
TCGGACCTGCCGGCAGCGGTGGAACTGAAGANGCTGGTCT
Clone Rv268
:::::::::::::::Rv268SP6.seq:::::::::::::::
AGCTTCAAGGACATCGTCATCGCGACCAAAACCGCGAGCTAGGTCGGCATCCGGGAAGCATCGCGACACCGTGGCGCCGAGCGCCGCTGCCGGCAGGCCGATTAGG
CGGGCAGATTAGCCCGCCCGCGGCTCCCGGCTCCGATTACGGCGCCCCGAATGGCGTCACCGGCTGGTAACCACGCTTGCGCGCCTGGCGGCGGCCTGCCGGATCA
GGTGGTATATGCCGACAAAGCCTGCGTGATCGGTCATCACCAACGGTGACAGCAGCCGGTTGTGCACCATCGCNAACGCCACCCCGGTCTCCGGGTCTGTCAN
:::::::::::::::Rv268T7.seq:::::::::::::::
GCTCGCGGTCCAGCAGCAGACGTGTCTGACCCCGACGCCCGGCCGCCGGTACCGAAACCGGATCGGCCCGCCGATGGCCGCGGCCACGGCGTCTGCCTTACCCGGC
CCGGATACCAGCAGCCACACCTCGCGGGAACGCTGAATCGCCGGCAGGGTCAAGGTGATTCGGCGTGGCGGCGGTTTCGCGAATCGTCCACCGCCACCACCATGCG
GGTGCTCTCGAAGACGCGGGGCTGTGCGGGAACAGCGAGTTAATGTGGCCCTCGGGCCCCATGCCCAGCAGGTGGACGTCGAAATTCGGCCCGGGTCACCTGGTGC
GGCACTGGCGGCC
Clone Rv269
:::::::::::::::Rv269SP6.seq:::::::::::::::
AGCTTGTCGATCGTCCGGCAGCGTCCGGCGAGTCAAGTCGAAGCCAGTCCGGTCTCCTCTCCGACTACGGCCAAGAACTGGGCGACGGTGTCAGTGCATACCAGCG
GANACTGGTGGCGCCCTAGGCGAGCGACCGCCTCACAAACGGCGGTGACCGCGTTCTGGTCGTGCACCATCGAGCCGTGCCCATCCCGGCCGCGTGCCGTCAGCCG
CATCCACTGGATGCCCTTCTCGGCGGTTTCAATCAGGTACAGGCGACGTTCGCCANCATCGTGCCGGGGCANGG
:::::::::::::::Rv269T7.seq:::::::::::::::
TTGGTGATCATCGNCCCAACGACCCCGAGGCGATGTTCTTGCACACCGAGGAGTGTCGCAAGCTGGGGCTGGCCTTCGCCGCCGATCCGTCTCAGCAGCTGGCGAA
GCTGTCGGGGTGAGGAAATTCGCAGGCTCGTCAACGGTGCTGCTTACTTGTTCACCAACGACTACTAATGGGATCTGCTGCTGTCCAAGACCGGCTGGTCAGANGC
CGATGTGATGGCGCAGATCGACCTGCGGGTGACCACATTGGGTCCTAAGGGTGTCGATTTGGTAGAACCTGACGCACCACCATCCACGTCGGCGTTGGTCCCCGAA
ACAGCCAGACCGA
Clone Rv26
:::::::::::::::Rv26SP6.seq:::::::::::::::
GGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTCAAG
CTTGATTTTGATCATCATGATGATCATCACCCGAAGTGTGGTAGCCGCAGTGGTTATCGTGGGTACCGTCGTGCTTTCCATGGGCGCCTCTTTGGGGCTTTGCGTA
TTGGTCTGGCAGGACATTCTGGGTATCGAGTTGTACTGGATGGTGTTGGCGATGTCGGTGATCCTGCTCCTGGCGGTGGGATCCGACTACAATCTGCTGCTGATTT
CCCGGTTGAAAGAGGAAATTGGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGACGGCTGCCGGCATGGTGTTCGCCGTTAC
CATGTCGTTGTTTGTGTTCAGCGATTTGCGAATT
:::::::::::::::Rv26T7.seq:::::::::::::::
CAGGCATGCAAGCTTGGCGTGCCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACGACGAGGTCGTCCGCGGGACACACC
TCGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCG
GATCGCGGTGATCGTCGATGACGGCATCGCCACCGGAGCGACGGCCAAGGCGGCGTGCCAGGTCNCCCGGGCGCACG
Clone Rv270
:::::::::::::::Rv270SP6.seq:::::::::::::::
GGCATCTTGGCCGCCATGTTAGCCACACTGCCACCGGCTATAGAAGCGATGCGCACCGTCCTGCCAGCACATTGCGGCGCTCCTCCCTGGAAAGCAAGATAACCAA
GCTCATGCCGTGGTTGTGGGTGGCGTGGTTTGGTTTGGGTAACTTTGG
:::::::::::::::Rv270T7.seq:::::::::::::::
TCGGCTAATAATCGTCGACGCCGGCCTCCTCTGCAATCGCCTTGGCGGTCGCCGGGTTGTCACCGGTGATCATCACGGTGCGGATGCTCATTCGGCGCATTTCGTC
GAATCGTTCCCGTATGCCCACCTTGACGATGTCCTTCAGATGGACGACGCCGATGGCCCGCGCGCTGCTGTTATCGGTCCATTCCGCAACGACTAGGGGTGTCCCC
CGCCGGAGCTGATGCCGTCGACAATGGCACCCACCTCCTCGGTGGGGTGGGCACCGTGATCGCGAACCCACTTCATCACCGCAGCCGCGGCACCTTGCGGATTCGA
CGGATG
Clone Rv271
:::::::::::::::Rv271SP6.seq:::::::::::::::
CTCAAGCTTGGAGGCGTGGCGATCGCGGTCCAAGGCGCGCTCTCCGAGCACAACGAGCGAAGACNGCTCGGCGACGGAGCCTTTATCGACNTCCGTTCGGGCTGGC
TGACGGCGGCNAAATAATGCTGGACTCGTTGTTGTCGACGGTGCCGTGGCGAGCCGAGCGCCGTCAGATGTACGACCGGGTGGTCTATGTGCCGCGGTTGGTGAGT
TTCCACGACCTGACCATCGAAGATCCGCCGCATCCGCTGCTGGCGCGGATGCGCCGGTGGCTCAACTAATTCTACGGCGGCGAACTGGGTNATCCCTTCNCCACCG
TCGG
:::::::::::::::Rv271T7.seq:::::::::::::::
CCTAGGTCAACCGTACCGTCATCGGATCGGGGTCGACCGCACAGATGGACTGGAGCTTCGGCGAGGTCATCGCCTATGCCTCGCGGGGGGTGACGCTGACCCCGGG
TGACGTGTTCGGCTCGGGCACGGTGCCCACCTGCACGCTCGTCGAAGCACCTCAGGCCACCGGAAATCATTCCCGGGCTGGCTGCACGACTGCGACGTGGTCACCC
TCCAGGTCGAAGGGCTGGGCGAGACGATGCAGCCGTCCGGACGAGCGGCACTCCTTTTCCGTTGGCTCTTTCGGCCGAATCCGGACGCCGAACCCGACCGGCGCGG
GGTCAACCCGGCACCGACGCGGGTGCCGTTTACCCGCGGGCTGCACAAATCCCGACGGGTATGGGCTTTGACCTGCCGACGGGGGA
Clone Rv272
:::::::::::::::Rv272SP6.seq:::::::::::::::
AGCTTGGCGTGACACCAACACAGGGCACTTAAGATGGCAATGCGCCGCCTACCTGCACGTTTTCGCGATGTCAGAGGATGCCGAGGGGAGAACAATGCGAGCACGG
CCGCTGACGTTGCTCACCGCTTTGGCGGCGGTGACATTGGTGGTGGTTGCGGGCTGCGAGGCCCGAGTCTAGGCCGAAGCATATAGCGCGGCCGACCGCATTTCGT
CTCGACCGCAAGCGCGACCTCAGCCGCAGCCGGTGGAGCTACTGCTGCGCGCCATCACGCCGCCTAGGGCTCCGGCGGCGTCGCCGAACGTCGGGTTTGGCGAACT
GCCTACCCGGGTCCGGCAGGCAACCGAT
:::::::::::::::Rv272T7.seq:::::::::::::::
TCATGCCGTTGGACCGACCATCGGAGTTAGTTGCCGAACCGCGGGACCACCGCAAGCACCCGGTCCTGGTCGCGCACCGCGTCGGCCAACCGCTTGAGCACCACCA
CGCCGCAGCCCTCGCCGCGCACGAATCCATCCGCGTTGGCGTCGAAGCTGTTGCATCGGCCGGTCGGTGACAGCGCCGACCACTTGGACAGCGCGATGGCGGTGAA
CGGTGACAAGGTGAGCTGCACCCCGCCCGCCAATGCCACGTCGGTTTCACGCAGGCGAAGCTCTGACACGCCAAGTGAATTGCCACCAGCGACGACGAACAAGCGG
TATCTACGGCGATGG
Clone Rv273
:::::::::::::::Rv273SP6.seq:::::::::::::::
GGGTCGACTTTCTGCAAGGCGAGGCTACACCGTCGTCGTCGTGGTATGCGATAGCCATCCCGTCGGGCTACTCGCCATCACCGATCAGCTTCGCCCCGAAGCCGCC
GTGGTGATTTCCGCTGCGACCAAACTGAACGGGGCCAAACCGGTATTGCTTACCGGCGACAACCGGGCCACCGCCGATCGGCTCGGTGTTCAGGTTGGCAT
:::::::::::::::Rv273T7.seq:::::::::::::::
AATCCGAAATCCTGACCGATACTTGAACCTGGTCTCGTTCGGCAATAACTCGTCGGCGTGCAGGACGCGGCGCAAACGTACTTCGGCATCAACGCGTCCGACCTGA
ATTGGCAGCAAGCGGCGCTGCTGGCCGGCATGGTGCAATCTAACAGCACGCTCTTCCCGTACACCAACCCCGACGGCGCGCTGGCCCGGGCGGAACGTGGTCCTCG
ACACCATGATCGAAAAACCTTCCCGGGGAGGCGGATGC
Clone Rv274
:::::::::::::::Rv274SP6.seq:::::::::::::::
TTCCGAATTTCGGGTCCNGGTCATATGACCCTCATGGAAGAAGAAGCGGCCGCCCCGCGCCCGTGCGACGGCGAATGAAAACCCTCACCCAGGCCGCATTGAACGC
CGACAAGACGGTGGAGCAGGTCGAAGACGTCCTGGACGGTCTGGGTAAGACCATGGCCGAGCTGAACAGCTCGCTGTCACAGCTGAACAGCACCGTGGAGCGCTTG
GAGGACGGTCTGGACCATCTCGAAGGTACCCTGCACAGCCTGGACGATCTCGCGAAACGGCTCATCGTGTTGGTCGAGCCGGTGGAAGCCATCGTCGATCGGATCG
ACTACATCGTGAGCCTCGGCGAAACGGTGATGTCACCGCTGTCGGTC
:::::::::::::::Rv274T7.seq:::::::::::::::
NCTCGATCTTGGGGTACGTTCGATGAGGCTGCTGACCAACAACCCGGCCAAGCGGGTGGGACTGGATGGATACGGATTGCACATCATCGAGCGCGTGCCGCTGCCG
GTGCGGGCCAACGCGGAAGAACATCCGTTACCTGATGACCAAGCGTGACAAATTGGGGCACGACTTGGCTGGGTTGGACGATTTTCACGAATCCGTGCATCTGCCC
GGAGAATTCGGCGGTGCCTTGTGAAGGTGGCGCCGGGGTGCCGGATCTGCCGTCGCTGGATCGTCTGGTGTGCGGCTGGCGATTGTCGCCAGCAGCTGGCACGGAA
AGATCTGCGACGCGCTGTTGGACGGCGCCCGCAAGTGGCCGCCGGTGTGGCCTCGATGACCGACTGTGGTCTCGGGTGCTCCGCGCGATCGATAT
Clone Rv275
:::::::::::::::Rv275SP6.seq:::::::::::::::
TCATCCCGACCAAAACGCGAGCTAGGTCGGCATCCGGGAAGCATCGCGACACCGTGGCGCCGAGCGCGCTGCCGGCAGGCCGATTAGGCGGGCATATTATCCCGCC
GCGGCTCCCGGCTCCGAGTACGGCGCCCCGAATGGCGTCACCGGCTGGTAACCGCTCTTGCGCGCCTGGGCGGCGGCCTGCCGGATCAGGTGGTAGATGCCNACAA
AGCCTGCGTGATCGGTCATCACCAACGGTGACAGCAGCCGGTTGTGCACCAAGCGCGAACGCCACCCCGGTCTCCGGGTCTGTCCAACCGATCGACCGCCCAAGCC
CACATGAACAAACCCCGGCATCACGTTGCCGATCGGCATACCGTGA
:::::::::::::::Rv275T7.seq:::::::::::::::
TTGGCGGGTTGGCCCAGCAGCCCGCCGGTGACGGCGACGATGCTGGGCTGGTTGCGGCCCTGCGCCACCGCGGCTTGCATGCTGGTTGGCTGTCTTGGGACGATCC
CGAAATAGTCCACGCGGATCTGGTGATTTTGCGGGCTACCCGCGATTACCCCGCGCGGCTCGACGAGTTTTTGGCCTGGACTACCCGCGTGGCCAATCTGCTGAAC
TCGCGGCCGGTGGTGGCCTGGAATGTCGAGCGCCGTTACCTACGTGACCTGATGGATCGGGGGGTGCCGACCGTGCCCGGCGATGTGTATGTGCCGGGANAGCCGG
TCCGGTTGCCACGCAAAGGCCATGTCTTCGTCGGTCCGACCATCGGTACCGGGACACGGCGCTGTATTGCCCGGTTCGCTGCCGAGTTCGTCGCGCAACTGCACGC
NGGCGGGCCAGCGGTGCTCGTTCANCCCGGAGGTTCCGGTGACGATGATCGTGTTGGTCTCCCT
Clone Rv276
:::::::::::::::Rv276SP6.seq:::::::::::::::
GTAGGAGAGAACAAAGACCGTCGATAGGACACGTGTTACGCCGGTAGCTGTCATTGGTATGGGGTGCCGCTGCCGGGGGGCATCTACTCACCCGATCGGTTGTGGG
AGGCGTTGCTGCGGGGCGACAATCTGGTCACCGAGATCCCCGCCGACCGCTGGGACATCTACGAGTACTACGACCCCGAACCCGGCGTGCCCGGACGCACCGACTG
CAAATGGGGCGCGTACCTCGATAACGTCGGCGACTTTGATCCCGAGTTCTTCGGGATCGGGGAGAAAGAAACGATAGCGATCGATCCGCAGCACCGCTTGTTGCTG
GAAACCTCCTGGGAAGCCATGGAACACGGCGGGCTAACACCGAACCATATGCCTCCCGACAMGGGTTTTCGTGGGGTT
:::::::::::::::Rv276T7.seq:::::::::::::::
CGAACTGAGCCCATAGAAAGGCAGCGACTAATTCGCTGGGCAAATAGGAAGACCCTTTGTCCTGCCACGTATATTTGTCGACCTCGTTGCGAAGGAAGCGGCTGCG
ATTGGTGCCCTTTTCCCTGGAGAATCTCTGCCCGGAGCAGGAAGTCTTATGAGTTGACAAGCAGGGGCGCCGCCTTCGCCGGAAATCACATTCTTGGTCTCGTGAA
ATGAGAGCGCTCCCAGGTCGCCGATGCTGCCGAGCGCCCGCCCACGATACGACGCCATCGCGCCTTGGGCCGCGTCTTCGACCACCGCCAGGTTGTGGTGCGTGGC
GATCTTCATGATCGCGTCCATCTCGCAGGCCACCCGGCATAGTGAACGGGGACCATGGCCTCGGTTCGCGGGTGAA
Clone Rv277
:::::::::::::::Rv277SP6.seq:::::::::::::::
CTTAGACGCCACCTCCGGGCCGAGCTCCACGGGGTGGATAAGTACGGCCGGATGTGGCCGCAATGGGAAGTTGTTGCCCGCTTGACTGTCCGGGTTAACGCCGGAT
TCCACCACATCCCCTTGCGAAAGGCCGTTGGGTT
:::::::::::::::Rv277T7.seq:::::::::::::::
GATCGCGATCGTCGATGTGGCCATCCGGCTTGGCGTCGACCCGCGTAAGGCAGACCAGATGGTTCGCGGCACGGTCAACCTGCCACACGCACTGGTAAGACTGCCC
GCGTCGCGGTATTCGCGGTTGGTGAAAAGGCCGATGCTGCCGTTGCCGCGGGGGCTGATGCTGTCGGATCGACGATCTGATCGAGAGGATCAGGGCGGCTGGCTGG
AATTCGATGCCGCGATCGCGATACCGGATT
Clone Rv278
:::::::::::::::Rv278SP6.seq:::::::::::::::
AGCTTACGCCGCTTTCGCTTCNGATTTGGGACGCCGCATCGAAAGCGCAGTTGGAAGCGCGGCGCCCGGCTGGTCGAGCTGCTCAAGCAGCCGCAATCCCAGCCCA
TGCCCGTTGAGGAGCAAGTGGTTTCGATCTTCCTGGGCACCGGCGGTCACCTGGACTCGGTGCCCGTCAAGGATGTCGGCGGTTCGAAACCGAATTACTGGACCAC
ATGCGGGC
:::::::::::::::Rv278T7.seq:::::::::::::::
CGACGGGACCTCGTCGCATCTTCCATAGCCCGCCACACCTTCAGTTGCTCACCGGAATCCAACCGGTATAAGGTCGGCGAAGCGCTCGGCATTGGTCATCGGGATA
TGCCGCTCGGGACGGTCAGATGCCCTCGGGTCCNGCCAGCACTCCTCAGGCTTCGTCGGGGTGGTCGCGACCGCATGGGCCACATCGCATTCACCAGGTCTGCGCG
AATCACCAGCACGTANACGGTTCCTTTCCTAAGCAACACCGAAATTTCAGGACCCGAATGCTCCGGGAAAACATGTCACGGTAAGTCCGGTATTCCGGGTACCGGT
TGAGCATTGA
Clone Rv279
:::::::::::::::Rv279SP6.seq:::::::::::::::
CGGCATCGGTTTGGGCTGTCACCAGCAGTTGGTACTTCTTCACTACTGTTGTTCGAGCGTCGAGCCGCCGCGCGTGTCGAGGTCGCCGGACGCGTACCCGCCAGGC
CGGTCAGGGTGCCCTTCCAGTCCACGCNGCTGTGGTCGGCTAACCGCTTATCTTCAATCGAGACNATCGCCAGCTTCATCGTGTTGGCGATCTTGTCCGAGGGCAC
CTCGAACCGGCGCTGCGANTACAGCCACGCGATCGTGTTGCCCTTCGCGTCGACCATCGTCGATACCGCAGGCACTTGCCCCTCGAGCAGCTGGGCCGATCCGTTG
GCAACGACCTCAGAGGCACGATTGGACATCAGCCCTAGCCCGCCTGCG
:::::::::::::::Rv279T7.seq:::::::::::::::
CCGTCGANGCCGCCGACTTGGCTTGACCGACACCAACATGGCCTGAGGGTGTTCAACAAGACCGTGGCCGACGGGCTGAACATCACCATGAGCGGCATGAGCCACG
CCACCGAGTTCATCATGTTGATCGCCGAAAACCATTGGCGGGTAGCGGAAGAACGGTCGAGGTGCTCTACACCCGAGTATTCGAAGTCGAAAGGCCAACCGTGCTC
AACGGCGTCAACATCATTTTCGACGGGTTTCTGCGAGGGAGGATGCCACGATGAACTGGATCCAGGTGCTGTTGATCGCGTCGATCATCGGGTTGCTGTTCTACCT
GTTGCGGTCGCGCCGAAGCGCGCGGTCCGTGCCTGGGTCAAGGTGGGCTATGTCTTGTTCGTGCTCCCGGCATCTATGCCGTGCTGAGA
Clone Rv27
:::::::::::::::Rv27SP6.seq:::::::::::::::
TTACACGNCCTGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGAC
ACTATAGAATACTCAAGCTTTTTGAGCGTCGCGCGGGGCAGCTTCGCCGGCAATTCTACTAGCGAGAAGTCTGGCCCGATNCGGATCTGACCGAAGTCGCTGCGGT
GCAGCCCACCCTCATTGGCGATGGCGCCGACNATGGCGCCTGGACCGATCTTGTGCCGCTTGCCGACGGNGACGCGGTANGTGGTCAAGTCCGGTCTACNCTTGGG
CCTTTGCGGACGGTCCCGACGCTGGTCGCGGTTGCGCGGCGGAAAGCGGCGGGTCGGGTGCCATCAGGAATGCCTCACCGCCGCGGCACTGNACGGCCAGTGCCGC
GGCGATGTCNGCCATCGGGACATCATGCTCGCGTTCATACTCCTCGACC
:::::::::::::::Rv27T.seq:::::::::::::::
CAGGCATGCAAGCTTTGTCACACCAAGTGTTTCGACCAGGCGCTCCATCCGGCGAGTGGATACTCCCAGCAGGTAGCAGGTCGCCACCACGCTGGTCAGTGCGCGT
TCAGCTCGCTTGCGGCGCTGCAGCAGCCAGTCCGGGAAATAGCTGCCCTGGCGCAGCTTGGGGATCGCGACGTCGATGGTTGCGGCACGGGTGTCGAAATCACGGT
GGCGGTAGCCGTTGCGCTGATTGGACCGCTCATCGCTGCGTTCGCGGTAGCCCGCCCCGCACAGGGCGTCGGCTTCAGCCCCCATCAAGGCGG
Clone Rv280
:::::::::::::::Rv280SP6.seq:::::::::::::::
AGCTTAGCCAGTTTTTCTACTCTTGGGCCCACACCCACAGTGCTTCGACGGTACGGTCACCCATGATGGCCATCCAGTTGGCATCGGTGAGCTGATAAATGCCAGC
TGGTTTCGCCAACCCGGTAGCGATCTTGGCGCGCTGCTTGTTGTCACTGATACCTATCGAGCAAGACAGCCCGGTTTGCGACAAGATGACTTTTCGGATCTCTTCG
GCGACTTCGATGGGGTCGTCGGGAGTCCCGGGCGCCACCGCGAGGTAAGCCTCGTCCCAGCCCCATACCTCGACCGGGTATCCCAGGTCGCGCAATAACGCCACCA
CCTCCTCGGACGCCGCGTTGTAGGCGGCTGGGTTCGACGGCAAGAAGTGGCCTCAGGGCATCGTCGGCGCGGTCCCAACGGCNTGCCGGCGCGCACACCGTAGGCG
CGGGGCTC
:::::::::::::::Rv280T7.seq:::::::::::::::
CCGGCGGAACTCAGACGTGCTGGTGGTGCGGCATGGCACCGCGGGCAGCAAAGCGCACTTCTCGGGGGACGACAGCAAGCGACCGCTAGACAAGAGGGGTCGTGCG
CAGGCAGAAGCGTTGGTACCACAGCTGCTGGCGTTCGGCGCCACCGATGTTTATGCCGCCGACCGGGTGCGCTGCCACCAGACGATGGAGCCACTCGCCGCGGAAC
TGAACGTGACCATACACAACGAGCCCACCCTGACCGAAGAGTCCTACGCCAACAACCCCAAACGCGGCCGACACCGAGTGCTGCAGATCGTCGAGCAAGTAGGCAC
ACCCGTGATCTGCACGCAGGGCAAGGTCATTCCCGATCTGATCACGTGGTGGTGCGAGCGCGACCGTGTGCCCCCGACAGTCCCGCAATCGCAAAGGCAGCACGTT
GGTGT
Clone Rv281
:::::::::::::::Rv281SP6.seq:::::::::::::::
GTATGGTCAGCTGTCCATCCGGCGCTGTCGGCCGAGCTGCCAGATCTCGTCAGCCGTAACCGGTTGCGGGATCCACGCGTGCGGGTTGTCTAC
:::::::::::::::Rv281T7.seq:::::::::::::::
CCGACTTTCCGCGGGTACCCGCTCAACTTTGTGTCNACCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGA
CGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATC
GTGGGGACCCACTGGCGAACCTGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGCGACCCGGCCTATGGTTATTCGACCTCGGCCGCCCAATGTTGC
GACTCCGTTCGGTTGTTCCAGAANGTCAGCCCG
Clone Rv282
:::::::::::::::Rv282SP6.seq:::::::::::::::
GCACCGATGTCGGCGAGCACTTCGTCAACTTCCAGGGGTGCCCGCACCAAGTATTTCGACGAGTATTTCCGTCGGGCCGCCGCCGCCGGTGCGCGGCAGGTGGTCA
TCCTGGCGGCGGGGCTGGGACTCGCGCGCGTACCGGCTGCCTCGGC
:::::::::::::::Rv282T7.seq:::::::::::::::
TGCACCCAACTTACTGAGCATGCTAACGCTGGTCGTGCGGGTCTTGTTCCCGCGTGTCGGCAGGGCACACGCTCGGGGCGTAGCTGGGAGAGGCCCCGGTCAAGCC
CGGAGAGCAGTGCTCAGTCCGCCAGCTTGACCGACTTTCGATGAGAACGCGCTTCTCGCCGTATTGAACTGGCGTGCTGACGGTCGCTGAGCAGCGCTCGCCGAGT
GCGGCCGCTGATTCTTTCATCGAGCCAGGACGCGCATTCGTGTTCGGCCGC
Clone Rv283
:::::::::::::::Rv283SP6.seq:::::::::::::::
AGCTTACGGCCGGTCGACGCGACGAGTGGTTCATGACACCACAAACCGTCAACGCCTACTACAACCCGGGGATGAACGAAATCGTCTTCCCGCAGCGATTTTACAG
CCACCATTTTTCGATCCGCAGGCCGACGAGGCCGCCAACTACGGCGGGATCGGGGCGCGTGATCGGGCACGATGATCGGGCACGGTTTCGACGATAGGGCGCCAAA
TACGANGGCGACGCAATCTGGTCNATTGGTGGATCGA
:::::::::::::::Rv283T7.seq:::::::::::::::
ATGCGTCACGTCACCACAATCGCGAGGACCCAATCATGCCGCCCAGGGCGGCCAACCCAATGGTGGCCGCGAAGCGGCAGCTCGATCGCAGCGCCGGAGGTGCCGG
CCGCCAGTTGATTCACGAACAGGGTGAGGTCATAGGCGGGCAGGATAGTGACGAACGCAAGACCTATATCTGCCGTCGGAGTAAGAATCGAGTAGCCGGTCGACCA
ACGGAAGCGAAAGTGTCCGCGATGTTGATGAGCGTCGCCGGTTGTGGCGGCGGTGGC
Clone Rv284
:::::::::::::::Rv284SP6.seq:::::::::::::::
AGCTTCACCAGCGTGCCGATGCTGTTCGCNACACCTCCCTACTATGCGCAATTCGCCGACACGGGTGGCATCAACACGGGCGATAAGGTGGACATCGCTGGGGTGA
ACGTCGGGCTGGTGCGCTCGCTGGCAATCCGCGGCAACCGCGTGTTGATCGGATTCTCGTTGCCCGGCAAGACAATCGGGATGCAAAGCCGGGCAGCAATTGGCAC
CGACACCATTCTTGGCCGTAAGAACCTGGAAATCGAACCCCGCGGTTCGGAGCCGTTGAAACCCAACGGTTTCCTGCCGTTGGCGCAGAACACTACGCCATACCAA
ATCTATGACGCGTTCGTC
:::::::::::::::Rv284T7.seq:::::::::::::::
CTGCCGCGGTGGCGGTCAGCGCCTGGCAAGTCACCGCACCGCCGTCCGGTTCATCGGCAGGCTCCCCCGAAAAGGGCCCTGGCAACAGAAGGTGATCAATGAGCTC
CCGCAGACCTTCGCCGATCTGGGACCGACATACGTGAAGTTCGGCCAGATCATCGCGTCCAGCCCGGGAGCATTCGGTGAGTCGCTGTCGCGGGGAATTCCGCGGC
CTGCTCGACCGGGTGCCGCCCGCAAAAACCGACGAGGTGCACAAGCTCTTCGTCGAGGAACTCGGCGACGAGCCGGCCCGGCTGTTCGCCTCCTTCGAGGAAGAAC
CGTTCGCGTCTGCGTCCATCGCCCAAGTGCACTACGCGACCTGCGCAGCGGCGAAGAAGTGTGGTCAACATCCACGGCCGGGCATCCGCCGCCGCGTTT
Clone Rv285
:::::::::::::::Rv285SP6.seq:::::::::::::::
GATCGTGCCGGCCCCCCGGCGGCAGTAGCAGATCAGCTCGTCGAAATCGCGGCAACCAGTCCAGTCGATTTCCATACGGGCGCCGTCAATCAACTCTGCGAAGATC
GCGATCGGCACCGGAAACCGGCGAGCCGCGTCAGCCAGCGCAACCAGCACCGGGATCGGATGAATCATCAATATTATCAAGTGATTTCCTGATGGCATCGAGCTCG
GTGATCTTGGTCTCGGGGGCCAGCTCGCCGTCGGCGACGTGCGTCGATCCGGCGGCCGAGCGCATAGCCGCAAATAGTGCCGCTCGCTTTTCGCGCGGCAAGAGTC
GGATGCCGTAATATANGTTTCTGGCGGCCGTGCGCGTGATCNACTCGGTGATTCGATACGCCTGTTCATCTCGGTCATGCCGTCCTC
:::::::::::::::Rv285T7.seq:::::::::::::::
GGTGGCGCAATGACCGAAACCACCCCAGCCCCGCAAACCCCGGCGGCCCCGGCCGGGCCCGCACAATCGTTCGTGTTGGAGCGGCCCATCCAGACCGTTGGGCGCC
GTAAGGAGGCCGTGGTACGAGTGCGGCTGGTGCCCGGCACCGGCAAGTTCGACCTCAACGGCCGCAGCTTGGAGGACTACTTCCCAAACAAGGTGCACCAGCAGTT
GATCAAGGCACCCCTGGTCACCGTGGATCGGGTGGAAAGTTTCGACATCTTTGCCCACCTGGGCGGCGGCGGCCCGTCGGGTCATGGCCGGCGCGCTGCGCCTGGG
TATCGCCCGGGCATTGATTCTNGTATCGCCGGATGACCGGCCCGCGCTGAATAANGCCGGCTTCTTGACCGTGATCCACGCGCCACCGAACGCAAA
Clone Rv286
:::::::::::::::Rv286SP6.seq:::::::::::::::
CACAATAGATTACTCAAGCTTCGAACCAGCGGCCTTATCACGTATCCCCGCTGAGACCTTGACCCTTAGGGCCGAAGTGACTTCGCTGCTGCTATGCCGACACCCG
ATTTCCAGACGCTGCTGTTACACGACGGCCGGGCCGGTGGCCACCATCACGCTCAACCGCCCGGAACAGCTCAACACCATCGTCCCGCCCATGCCCGACGAGATCG
AGGCCGCTATCGGGTTGGCCGAGCGCGACCAGGACATCAAGGTCATCGTGCTGCGCGGTGCCGGCCGCGCCTTCTCCGGCGGTTACAACTTCGGCGGCGGGTTCCA
ACATTGGGGGCAT
:::::::::::::::Rv286T7.seq:::::::::::::::
TCAGGACGCTTATGGTTGGCAGATGGTCGCCCTGGCGTCGAATACGCGCGAGCGCATGAGCTCACCGGTTCGGAACAACGTATCGAAGAACGTCGCACTGCTGGCA
GATGGTATCTCCGATGTGGTTGTAATTTGTATCCCAACTCTAACTGTGCTATCGGATCAGCGTGAATATCGAGATATTGCGAATGCGATGACAGGCCGCCATTCGG
TTTATTCGCTTACGCTTCCCGGGTTCGATTCGTCTGATGCACTGCCGCAAAACGCGGATATGATTGTTGAAACCGTATCTAACGCAATTATTGATGTGGTAGGCGG
CAGCTGCCGTTTTGTGCTGTCGGGCTATTCATCGGGTGGGGGTGTTTGGCTATGCCCTCTGCTCCCAT
Clone Rv287
:::::::::::::::Rv287SP6.seq:::::::::::::::
CGCAGCTGTCGCCGATCTGGTCCGGAATACCAGCTCCAGGTTCTGAGTGGAAGATGAGTGCGGGCCATCGAAGTGTTGTCAATGTACTCCAGGATGTCAGGTGCCG
GCCGCTGGCGAGGATCTTGGGCACCGCCGCCATGACTTGGTCGAAGTCGGCGAACGGGGCGAGCACGCTGGCGTCGTGGTC
:::::::::::::::Rv287T7.seq:::::::::::::::
GTAGTTCGTTCATCCAAACACAGTGCGGTACCGGCTCAAGCGGATCACCGACTTCACCGGGCGCGATCCCACCCAGCCACGCGATGCCTATGTCCTTCGGGTGGCG
GCCACCGTGGGTCAACTCAACTATCCGACGCCGCACTGAAGCATCGACAGCAATGCCGTGTCATAGATTCCCTCGCCGGTCAGAGGGGGTCCAGCAGGGGCCCCGG
AAAAGATACCAGGGGCGCCGTCGGACCGA
Clone Rv288
:::::::::::::::Rv288SP6.seq:::::::::::::::
TCCGCTCGCTTCTCCGAGAGGTTGAGTGCCAACGCTCTGCCGATGCCCGAAGCCGGCCCCGGTGATGACGGCGACCTTGCCTTCGAATGAGCTCATTTGACTACTC
CCCGTGGTTGTCCCTGCGATTGGTGGAGGTGGCCGCGCAGCCTTGCCCCGAGGTCGGCGATCGCGTCTCGGGCTTCGGGGAGCAGACTGACCTGCAGATGGAAGTC
GTGCCACATGCCCGCGAACCGGCGATGCTCGATGCTTGTTTTCGAAGCGGCGCAGGCGGTTTCGATCTTGTCCGCGTCAACACNGATCGGATCGTCGCCCGCGGTC
TGCATGACGAATGGGCG
:::::::::::::::Rv288T7.seq:::::::::::::::
ATGGGAGGCCACCGATTACCATCTTGCACACACCGATTCCGGGCTATTGATGTCCACGTTCGGTCCGCGAACCGCGCTGTGGCTGCTGCTGGCCAAAGGCGGAGGC
GATACCGAAGTCAGTGCCCAAGCTTGGGTTCCACGCTCGCGCAGCCACGCCGTCACCTTTCCACGAGACCTCACCTGCCGATCCGAAATGGAATCGGCCGTGACGG
AATTGGCGCAGCGAACACTCAACGAGGTGGTGGCTTCGTCGCGAACCGTCACCCGAGTCGCGGTCACCGTGCGCACGGCGACGTTCTACACCCGCACCAAGATCCG
AAAGCTGCAAGCTCCCAGCACCGATCCCGACGTCATCACCGCTGCCGCCCGGCACGTTCTTGAACCTATTCGAGCTGGAATCGGCCGTCCGGTTGCTGGGAATTGC
NGTTAAGAACTGGGCCT
Clone Rv289
:::::::::::::::Rv289SP6.seq:::::::::::::::
GCTTTGCGCGCTTCTCCGAGAGGTTGGAGTGCCAACGCTCTGCCGATGCCCGAGCCGGCCCCGGTGATGACGGCGACCTTGCCTTCGAATGAGCTCATTTGACTAC
TCCCCGTGGTTGTCCCTGCGATTGGTGGAGGTGGCCGCGCAGCCTTGCCCCGAGGTCGGCGATCGCGTCGCGGGCTTCGGGGAGCAAACTGACCTGCAGATGGAAG
TCGTGCCACATGCCCGCGAACCGGCGATGCTCGATGCTTGTTTTCGAAGCGGCGCAGGCGGTTCGATCTTGTCCGCGTCAACGCAGATCGGATCGTCGCCCGCGGG
TCTGCATGAAGAAT
:::::::::::::::Rv289T7.seq:::::::::::::::
CTCACGCAGCCACGCCGTCACCTTTCCACGAAGACCTCACCTGCCGATCCGAAATGGAATCGGCCGTGACGGAAATTGGCGCAGCGAAACACTCAACGAGGTGGTG
GCTTCGTCGCGAACCGTCACCCGAGTCGCGGTCACCGTGCGCACGGCGACGTTCTACACCCGCACCAACATCCGAAAGCTGCAAGCTCCCAGCACCGATCCCGACG
TCATCACCGCTGCCGCCCGGCACGTTCTTGACCTATTCGAGCTGGATCGGCCCGTCCGGTTGCTGGGAGTGCGGTTAGAAACTGGCCTAGAAACCGGCGGGCACAC
CGCACCTGGGCGGGGN
Clone Rv28
:::::::::::::::Rv28SP6.seq:::::::::::::::
TGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAAT
ACTCAAGCTTGATGCCGCCGAAACCGAGCGTGAGCACGCCGCCAGCCACCACNCGCGGGTCGGGCGCCGGGCCCGGGTCGCCANGCTGCTCCGCTCGGTGATGGCA
CGCCACCGCGACACCACCCGGCTGCGCTACGTCGAGCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCCTCTTTCGAAGTCGAAGTCGATA
CCGATTGCGCATCCGCNGCCGCA
:::::::::::::::Rv28T7.seq:::::::::::::::
CAGGCATGCAAGCTTCACGTCCGTACGGCTCGGGTACGCTTCGGTCGCAGTGTGCAGTGATAGATGACGACCGGGAACCTCGTCTGCATCTTCCATAGCCCGCCAC
ACCTTCAGTTGCTCACCGGAATCCAACCGGTAGAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAACCCTCGGGTCCGGCCA
GCACTCCGCAGGCTTCGTCGGGGTGGTCGCGACGCGCATGGGCCACC
Clone Rv290
:::::::::::::::Rv290SP6.seq:::::::::::::::
GCTTGTCTATCGTCCCGGCCAGGTCCGGCCAGTCAAGGTCGAAGGCCAGTCCGGTCTCCTCTCCGACTACGGCCAAGAACTGGGCGACGGTGTCAGTGCAGACCAG
CGGAAACTGGTGGCGCCCTAGGCGAGCGACCGCCTCACAAACGGCGGTGACCGCGTTCTGGTCGTGCACCATCGAGCCGTGCCCAGCCCGGCCGCGTGCCGTCAGC
CGCATCCACTGGATGCCCTTCTCGGCGGTTTCAATCAGGTACAGGCGACGTTCGCCACCATCGTGCCGGGGCACGGTTAGCGAGAAACCGCCGACTTCACGATTGC
CTCGGTGATGCCGTCGAAACAGATCGGGCCT
:::::::::::::::Rv290T7.seq:::::::::::::::
GCGCGCCATGTTGAGGTTGTCCGACGGTGACGACGGTGAACCACAACTGTTTGACCTGTCCGCACACACCGTGTGGATCGGCGAGCGGACCCGACAAATCGATGGC
GCGCACATCGCGTTTGCCCAGGTGATTGCTAATCCGGTCGGGGTCAAGTTGGGCCCCAACATGACCCCGGAACTGGCCGTGGAGTACGTCGAGCGGCTCGACCCGC
ACAATAAGCCGGGCCGGCTGACTTGGTGAGCAGGATGGGCAACCACAAGGTCCGCGATCTGTTGCCACCGATCGTGGAGAACGTCCATGCCACCGGGCATCAGGTC
ATCGGC
Clone Rv291
:::::::::::::::Rv291SP6.seq:::::::::::::::
TTGCCTTCCATGCCGAGCAAGGTCGACTCAGCGATGACGAATTGTTCTTCTTCGCGGGTGTTGCTGCTGGTTGCGGGCTATGAGAGCACTGCTCATATGATTAGCA
CATTGTTTCTGACGCTGGCCGACTATCCAGATCAGCTGACACTCCTTGCGCAGCAACCAGACCTGATCCCGCCGGCGATCGAGGA
:::::::::::::::Rv291T7.seq:::::::::::::::
CGACGCTGGGCCCAACTGCGACCACCAGGTCCTGGTATGGCAGGACATGGCCGGGTTCAGCGGCGCCAATACCG
Clone Rv292
:::::::::::::::Rv292SP6.seq:::::::::::::::
TAACGACTCGGGTCCAGCGACCGCGCCAACACNAACGGCCGGACNACGTGGGCCAGGGTCGCGGCCTCCCCTACAAACAGGATCCGTTGCCTGCGAACGACAGGCT
CCGGTGCGGCGTTGGGCGCCGTGCTCGTCCCAGCGTCCGGTCCCGGGTCGCCGGCGACGCTTGTTTCCTCCATACTCGCCCCCTAATCTCGAGGCAGCCCGTACCC
GCAGGCAACCTCCCAAAAATGCAATCCCCCAAAATGCAATGCGTCNAGCTATTTCTCACACCGACCGCTAGTTGCGGATCANAAATCCGTTGGGCGCGGA
:::::::::::::::Rv292T7.seq:::::::::::::::
CNTGGCGGTGGGTGCGGTGTCGAACACGACCACACTTCTTTGCGGTTCGGTGATCTCGACACCGGCCGCGAGCCGACCACCATGCGCGCGTAGATCGGCGATCAGC
GCGTCGGCTATCGCCTGGGTGCCGCCCACCGGAATCGGCCAGCCGACCGAATGGGCCAGCGTTGCCATCATCAGTCCGGCGCCGGCCGACACCAGTGACGGCAACG
GTGAAATCNCGTGGGCGGCAACGCCGGTGAACAACGCGCGGGCATCCTCGCCCGCCAGCGACCGCCAGGCAGGGGTGCCCTGGGCCAGCATCCGCAGCCCGAGACN
CAGGACCGANCCCAGTG
Clone Rv293
:::::::::::::::Rv293SP6.seq:::::::::::::::
GCTTTTCNGATCGCAGCGAGTCGTACCCGCGCCGGTCACCTTCGTGGATATCGCCGGCCTGGTCAAGGGGGCGTCCGAGGGAGCCGGGCTGGGTAACAAGTTCCTG
GCTCATATCCGCGAATGCNACGCCATTTGTCAGGTGGTGCGGGTGTTCGTCAACAACNACTTGACTCATGTCACCGGACGGGTCGATCCCCANTCCGACATTGAGG
TCGTCGANACCGAGCTGATCCTGGCANATCTGCAAACCCTGGAGCGGGCCACGGGCCGGCTGGAGAAGGAANCGCGCACCAACAAGGCGCGCAAGCCGGTCTACGA
CGCGGCACTGCGTGCCCAGCAGGTGCTCGACGCCGGCAANACGCTGTTCGCCGCGGGGGTGGATGCCG
:::::::::::::::Rv293T7.seq:::::::::::::::
GTCGTACGCCATTNGTCGGTGTGCGCATACCAGTACGACGCGCCGGGCACCTGACGCGGCGGCCGCGACCAGTCGGTGGCCATCGCCATCGTCTGCCACCCGGTCA
ACGGACGCACCTTCTCCTGGCCGACGTAGTGCGCCCACCCGCCGCCGTTGCGTCCCATCNATCCGGTCAACATGAGCAGCGCCAACACCGAGCGGTACATGACATC
GCTGTGGAACCAGTGACAGATTCCGCCGCCCATGATGATCATCGACCGTCCTCCGGATTCGGTCGCGTTGCGGGCGAAATTCCTTGGCAAACCGGATTGCCTGCGC
GGCCGGCACACCGGTGATCGACTCCTGCCAGGCCGGGGTGTTCTGCTGGGTTCGGTCGTGGTACCGGT
Clone Rv294
:::::::::::::::Rv294SP6.seq:::::::::::::::
GCGAGGCGGTATCGCTTCCCGTCGTACCGGCGACCGCCAGCCGAGAAGCTCGTTTTCCCAGTGTTGCTGGGGATTCTCACGCTGCTGCTGANTGCGTGCCANACCG
CTTCCGCTTCGGGTTACAACGAGCCGCGGGGCTACGATCGTGCGACGCTGAANTTGGTGTTCTCCATGGACTTGGGGATGTGCCTGAACCGGTTCACCTACNACTC
CAAGCTGGCGCCGTCTCGTCCGCAGGTCGTTGCTTGCGATAGCCGGGAGGCCCGGATCCGCAATGACGGATTCCATGCCAACGCTCCGAGTTGCATGCGGATCGAA
TACNAATTGATCACCCA
:::::::::::::::Rv294T7.seq:::::::::::::::
TGGGTCTTGCCGGCGAGCCCAGCGAAGTCGCTAGCGTGGCCGTGTTTCTTGGCTTCGGATCTATCCTCGTTACATGACCGGCACCGTGTTGGACGTGACTGGCGGC
CGGTTCATATGACACCGAGATCATTGCCACGGTACGGCAATTCGTCAAGAAGGAAATCTTTCCCNATGCACCGGCCCTCGAACGTGGCAACAGCTACCCGCAAGAA
ATCGTCGATCGGCTGGGTGTTATGGCTTGCTCGGTCGCCGGCTGCAAGGGTATCGACACCACCGAGTTCATTCTCGGGCGTGCCGGCCGCATTCGAGCTGCCGGTG
CGCGCTGCCCAGCACCGTCATAAGTACTTGANGATGGTCAAACGTCGGACGAACCGCCACCACGTCGCTGCCGAACGG
Clone Rv295
:::::::::::::::Rv295SP6.seq:::::::::::::::
TAGATGCCCAAGCTTGCCNTTANAGACCTCGTCGACCAAGCACGGACGCGACCGTCGAAGGTGGCGAATCCGGGCTTGGCGTCNACCCGCGTAAGGCAGACCAGAT
GGTTCGCGGCACGGTCAACCTGCCACACGGCACTGGTAAGACTGCCCGCGTCGCGGTATTCGCGGTTGGTGAAAAGGCCGATGCTGCCGTTGCCGCGGGGGCGGAT
GTTGTCGGGAGTGACGATCTGATCGAGAGGATTCAGGGCGGCTGGCTGGA
:::::::::::::::Rv295T7.seq:::::::::::::::
TCTCCACGGCGTGGATCAAGGTACCGGCCGGGATGTTGCGCAATGGCAGGTTGTTGCCCGGCTTGATGTCTGCGTTAGCGCCGGATTCCACCACATCCCCTTGCGA
AAAGTCCGTTGGGTGCAATGATGTAGCGCTTCTCCCCATCGAGATAGTGGAGCAACGCAATCCGTGCGGTACGGTTCGGGTCGTACTCGATGTGCGCGACCTTGGC
GTTGACACCATCTTTGTCATTGCGGCGAAAGTCGATCATCCGGTAAGCGCGCTTATGACCGCCGCCTTTGTGCCGGGTNGGTAATCCGGCC
Clone Rv296
:::::::::::::::Rv296SP6.seq:::::::::::::::
GCCCGGTTCGATCGGGCATGTCCGCAGTCGTCGTTACCGGAGGCGGTCGTGGCCGCGCTAATCGGCGTCGGCGCCGACAAGATGTGGGATATCCGCAATCGGGGCG
TCATCCCTGCGGGCGCGCTCCCCCGCGTCCGAGCCTTCGTCGACGCAATCGAGGCAAGTCACGACGCGGATGAGGGGCAGCAGTGAATTACAGCGAGGTCGAGCTG
TTGAGTCGCGCTCATCAACTGTTCGCCGGAAACAGTCGGCGACCGGGGTTGGATGCGGGCACCACACCCTACGGGGGATCTGCTGTCTCGGGCTGCCGACCTGAAT
GTNGGTGCGGGCANCGCCGGTATCNACTCCCGTGGAACACAGCCGGGGC
:::::::::::::::Rv296T7.seq:::::::::::::::
CTCGGCGTGGATATCGGTGTAGCCGGCGCCGGTGAANGTCGGCTCCTTACGTCCACTCGACAACAGCTCATAGCGATCCAACCAGTANGCAACCGCCTTCAGCAGT
ACAACCGCGCCGGCGAACACTGCGAGTTGAACGCGAGCTGCCTGGGTCAGCATGCCTCTGCCGGTTGTCAGCCGAAGGCCGCCGAACAGGTAATGCGTCAACAGGC
TCGCTAGAAACGCCAGAACCACGGCCACGAACAGCCAGTTCAGCACCGACCGGTAGAACGGCAGATCGAAGACGAAAAAACCCAATGTCATAGCCGAATTCGGGGT
CCACGATGCCAAAGGTGCCCCCCGTGTACAACAACTGAACTTCACCCA
Clone Rv29
:::::::::::::::Rv29SP6.seq:::::::::::::::
TCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTC
AAGCTTCACGTCCGTACGGCTCGGGTACGCTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCACACCTTCAGT
TGCTCACCGGAATCCAACCGGTAGAAGGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCCGGCCAGCACTCCGC
AGGCTTCGTCGGGGTGGTCGCGACGCGCATGGGCCACCATCGCATTCACCAGGTCTGCGCGAATCACCAGCACGTAGACGGTTCCTTTCCTAAGCAACACCGAAGT
TTCAGGACCGAATGCTCCGGGAAACATGTCA
:::::::::::::::Rv29T7.seq:::::::::::::::
CAGGCATGCAAGCTTGATGCCGCCGAAACCGAGCGTGAGCACGCCGCCAGCCACCACGCCCGGGTCGGGCGCCGGGCCCGGGCCGCCAGGCTGCTCCGCTCGGTGA
TGGCACGCCACCGCGACACCACCCGGCTGCGCTACGTCGAGCCATACCGGGCGGAGCTCCATCCGCTCGGCCGCCAGTGTCCGGGCCCTC
Clone Rv2
:::::::::::::::Rv2SP6.seq:::::::::::::::
CCTGCATCCGGCTCGTATGTTGTGTGGAATTGTGANCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGA
ATACTCAAGCTTCCAATCCCCCTGCCCTGATACGCGTCGGCAACCGTGAACGCGATCTCGGCGACCGTCGGATCGGTTTCATCCCGCACAAAACGCGCGTCGGCTA
CGGGGTCGCTTCCGTCGGTCACCACCCAGACGAAGTGGTCGACGTAGTCGACTTCCGACAGGTAGTGCATCAACGCCGGACTGGGAACACNAGCCGACTGAACCCG
TCGATACAGCGTCTCNCCGGAGAACTGGATGTGTCCGTGCACGGTCCGCTCGCGGTCACCGGGCAGCACGGGGCGTAACATCAGTTGAGTCCCGTCGGCAAGCCGT
ACCGGAATCGGGGAGACGA
:::::::::::::::Rv2T7.seq:::::::::::::::
CAAGATGATCGCCGGTGCCACCCCGATCCGTGCCTCGGTCAGCGCGAACGTGCTTTCCGGTCCGGCGACCACCATGTCGCACGCACCGACCAGGCCGAACCCGCCG
GCCCGCACATGCCCGTTGATGGCGCCGACCACCGGCAGCGGCGACTCGACGATGGCGCGCAACAGCGCCGTCATTTCCCGCGCCCGCGCCACCGCCATCCGGTACG
GATCACCACCACCACCGCCGGCCTCGCTGAGGTCCGCGCCGGCGCAGAACGTTCCGCCGGTATGCCCCAGCACGACCAGCCGCACCGCCGGATCTGCTTCGGCCGC
ACTCAGCCCTTGATGTAGTTGGCTGACCAGCGTGCTCGACAGCGCGTTGCGGTTGTGCGGAGAGTTCAGTGTCAGCCTGGCGAAGGGGCCGCCGCAGGCGGCCGGG
CCAGCGTAGTCGACGGGGCTG
Clone Rv301
:::::::::::::::Rv301SP6.seq:::::::::::::::
CTCAAGCTTCGATCGACAGTACTCCCGCCTTGGGTCTGGTCTTCGAGCTGGTCGGTCATGGTCGGACCTGCTGGTAGTGGGGATCTAACGCAACATGGTCGGGATT
CATCATGGTGTACCCGTGATACCCATTCGCAGCTGCCGGTGAAACCCCGCGATGCCGGGATTTCCAGCCGACTAGGATGTCTAGCCGGCCAGCCGCTGCCGCCCGG
ACTTCGGGATGTTCGGTATACCACCGATCGGCAATCTTGCNTATCCGCCGATGCTCGAACGCTAGCCACCCCAAACCAACCACTGTGACNACAATC
:::::::::::::::Rv301T7.seq:::::::::::::::
TGAATTTCCCGATCCCACAATCTCGGTTCAGATACAGGTCGCCATACCCCTTACTTCGGCAACGCTGGGCGGATTGGCCCTGCCGCTGCAGCAGACCATCGACGCC
ATCGAATTGCCGGCAATCTCGTTCAGCCAATCCATACCCATCGACATTCCGCCGATCGACATCCCGGCCTCCACTATCAACGGAATTTCGATGTCGGAGGTCGTGC
CGATCGATGTGTCCGTCGACATTCCGG
Clone Rv302
:::::::::::::::Rv302SP6.seq:::::::::::::::
TACTCAAGCTTGAACGCTGCGAGCGAGCCCATGTAGAGCGTTTGGTACCAAACCGATCGGTGGGCCAACTTGCCATGGGCTCACAGCGGCTATCGCGAGCGTGTAG
CCGATCATCGGCCAGGCGACGGTGGCCTGAGCGGCAGGGGTTGCCTTATCCATCCTCTTGCGGCATGGTTGCCGCAGGGAGTGCCGGTAAGTCTGGTCGGCAACCT
GGCCCGCTGCGGGTTGGGTTCGGATTCCCTCGGCTAGTAAGGTGCTCGCCTGGTGTTACAACGAATCGCTAGACAGCTCTTATCGGGAGTGGCCGTCGCGATCGTT
GCGCTGCCGCTGGCGATCGCGTTCGGCNTTACCGCCACCGGAACGTCCCAAGGTGCGCTCATCGGGCTCTACGGCGCCATCTTCGCCGGATTCTTCCCNGCCGTGT
TCGGTGG
:::::::::::::::Rv302T7.seq:::::::::::::::
GCGGTGTCTGAACTTCGCCCGTTCCCTCCAGCGCATTGAGCTTCAGCCCGACCGGCAGGTAGGGAGTCGGCATGCGGTCCTTCGCCCCGAGCCCGCTGGCTAAATA
GCCACCCCCGAGCGCGGTCACGGTCTTTGCACCGGGACGACGGCATACCGGCAGCGCGAACATCGCCGCGGGCTGCAGCGTGAACGTCGAATACGAGTCGAACAGT
GTCGGCGCGTAAAAACCCGAGCCGGCGGTCGCTTCGGTAATCAACGGCTCCTGCGCAACCAGCTGCAANTCNCCGGTGCCACCGGCGTTGACAATCTTGATNTCGG
CGACCTCGCGCACCAN
Clone Rv303
:::::::::::::::Rv303SP6.seq:::::::::::::::
TACTCAGCTTCGGCTCAGGTGGTGCTGCTGGTAAAGTTCNCTGAACGGTGCAAGGTTTCGACAATGTGGTGCCGGTTCGGCGGGTACTGCCATCGAGACACTGGCG
AGGCTATCGCACCCGTTATCGGCTACAAACAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGAT
CGCCGTCAAGCNTGTGTGCCGCGGATTATCAGGACTGACCTCCTGGCTGACCGGCNTGTTTGGTCNCGATGCCTGGCGCCCGGCCGGCGT
:::::::::::::::Rv303T7.seq:::::::::::::::
CATCACCTGGTTCATGAAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCGGGTGCAGGTGCTCGGGCAGCTCGGC
CGCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACGAACGACGCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACANCGCGTTCTC
CACCGACCGGGCCCGGGTGTGGGGTGTT
Clone Rv304
:::::::::::::::Rv304SP6.seq:::::::::::::::
CTCAAGCTTCCCGGCGGCCAGTACCGAAAGCGCGAACAGCTCGCGGCAGCCCACAACNTGCTGCGTCGGATTGCCGGCGGCGANATCAATTCCAGGCAGCTCCCGG
ACAATGCGGCTCTGCTGGCCCGCAACGAAGGACTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCCGATCGCACAGGTTGGCCCACAACCGGCCGCTTG
ATGCCCGGTCGGCAAGCCCCGGCAGTTGCCAAACCCAGCGTGATCAGGCTCGGCTCGCGAGTTCGGCGAAGAAGTGGCTCGCCTGATCACCTACCATCGGCCAGGT
CTGCGTGTCATCACNACGCTCGCCAAGGAAGGTTGTTGTGGTGCT
:::::::::::::::Rv304T7.seq:::::::::::::::
GCCACGTTTCGCGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCGCGGGTAATCGCCGACGGTGCCGGTTCGCGAGCCGAAGGTGACGACGCT
GATTGAATCGAGTTCCAGGTCCAGCGGGTGGCGCAGCAACGGCGCGAGCTCAACGACGTCAATCACGTTGTCGCTTTCTACGGTCACCGACCCGGTGACCGTAGTC
GCCCGGTGCGCTCGGCCGAGAAGTTGCACCGCCACCACCGCGACACCGTCTTGCACGCGGACGCCACCCCCCGGATCGGTTGTTGGCCAAGGTAATTGGGTCATCC
ATTTGACGGGACGCCGACCCCGCAGCCCCAGTACCGCCCACGACCACGCCGGCTGACCCCACCACTGTACGAACACCAAGGCGACGCCGACCA
Clone Rv306
:::::::::::::::Rv306SP6.seq:::::::::::::::
CTCAAGCTTGATGCCGCCTAAACCGAAGCGTGAGCACGCCGCCACCCACCACGCGCGGGTCGGGCGCGGGGCCCGGCGCCGCAGGCTGCTCCGCTCGGTGATGGCA
CGCCACCGCGACACCACCCGGCTGCGCTACGTCAAGCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCCTCTTTCGAGGTCNAGGTCNATA
CCGATTTGCGCATCCGCAGCCGCACCCTGGACGACAGAACCGTGCCCTACGAGTGCTTGTCGGGCGGGGCCAAAGAACANCTTGGCATCCTGGCGCGATTGGCCGG
CGCGGTCCTGGTC
:::::::::::::::Rv306T7.seq:::::::::::::::
CTCGGGTACGCTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCGCCACACCTTCAGTTGCTCACCGGAATCCAACC
GGTANAANGTCGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCCGGCCAGCACTCCGCAGGCTTCGTCGGGGTGGTC
GCGACNCGCATGGGCCACCATCGCATTCACCAGGTCTGCGCG
Clone Rv307
:::::::::::::::Rv307SP6.seq:::::::::::::::
CTCAAGCTTCAATTCCTCCACGACGCGTTCCCAAATGAATTTCCCGATCCCACAATCTCGGTTCAGATACAGGTCGCCATACCCCTTACTTCGGCAACGCTGGGCG
GATTGGCCCTGCCGCTGCAGCAAACCATCGACGCCATCGAATTGCCGGCAATCTCGTTCAGCCAATCCATACCCATCGACATTCCGCCGATCGACATCCCGGCCTC
CACTATCAACGGAATTTCGATGTCGGAGGTCGTGCCGATCGATNTNTCCGTCNACATTCCGGNGGTCACCATCACCGGCACCAGNATCGACCCGATTCCGCTGAAC
TTCGACGTTCTCAGCAGCGCCGGAACCA
:::::::::::::::Rv307T7.seq:::::::::::::::
TTAACCCCCGTGGCCTCTACGCCGCCTNCGGGTCGAACATGCATCCCGAGCANATGCTCGAGCGCGCACCCCACTCGCCGATGGCCGGAACCGGCTGGTTACCCGG
GTGGCGGCTGACGTTCGGCGGCGAGGACATCGGCTGGGAAGGGGCGCTTGCCACCGTCGTCGAAGACCCAGATTCGAAGGTGTTCGTCGTGCTCTACGACATGACC
CCGGCGGACGAGAAGAACCTTGACCGGTGGGAAGGCTCCGAGTTCGGCATCCACCANAAGATCCGATGCCGCGTT
Clone Rv308
:::::::::::::::Rv308SP6.seq:::::::::::::::
CTCAAGCTTGATTTTGATCATCATGGATGATCATCACCCGAAGTGTGGTAGCCGCAGTGGTTATCGTGGGTACCGTCGTGCTTTCCATGGGCGCCTCTTTCGGGCT
TTCCGTATTGGTCTGGCAGGACATTCTGGGTATCGAGTTGTACTGGATGGTGTTGGCGATGTCGGTGATCCTGCTCCTGGCGGTGGGATCCGACTACAATCTGCTG
CTGATTTCCCGGTTGAAAAANGAAATTGGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGACGGCTGCCGGCATGGTGTTCG
CCGTTACCATGTCGTTGTTTGTGTTCAGCGATTTGCGAATTATTGGTCAGAT
:::::::::::::::Rv308T7.seq:::::::::::::::
CGNCCAACCCGAATTGGTTTTCGGCGCCNTCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACACCTCGATGCTGCCGCCATGGACGCG
GTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCNCNGGCGTTACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGACG
GCATCGCCACCGGAGCGACGGCCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCANACGACATCGTGGC
GAGATTCGCCGG
Clone Rv309
:::::::::::::::Rv309SP6.seq:::::::::::::::
CGTGACTGCCACCGGGGCCACTCCGCAGAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCGCGGTACCCGCTCAACTTTGTG
TCNACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACNCAGCGGTTCCNCTGACCAATACGGTCGGTC
CCACGATGACCCANTACTACNTCATTCGCACGGANAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCAACC
AAACTTGAAGGTGATTGTTAACCTGGGG
:::::::::::::::Rv309T7.seq:::::::::::::::
TCGCTCAAGCGCNTGAGGCCGAANCGGCTGGTTACGACTCCCTGTTTCTGATGGACCACTTCTACCAACTGCCCATGTTGGGGACGCCCGACCAGCCGATGCTGGA
GGCCTACACGGGCCCTTGGTGCGCTGGCCACGCGACCGAGCGGCTGCAACTGGGCGCGTTGGTGACCGGCAATACCTACCGCAGCCCGACCCTGCTGGCAAAGATC
ATCACCACGCTCGACGTGGTTAGCGCCGGTCGAGCGATCCTCGGCATTGGAGCCGGTTGGTTTGAGCTGGAACACCGCCAGCTCGGCTTCGAGTTCGGCACTTTCA
GTGACCGGTTCAACCGGCTCGAAAAGGCGCTACANAT
Clone Rv30
:::::::::::::::Rv30SP6.seq:::::::::::::::
ATACTCAAGCTTCCGCTGGGGCCTGTTCAACCATGGCGATCCCGTTGGTCCCGGACATCCCGAACGAGGACACCGCGACCCNCTTCGGTGTGTGATCATTACCGTT
GGGCCACTGCGTAACCGCTTGCGGCACAAAGAGCCCGGTCTCGACGTCGGAAAAGCTCATCGGGCACCCGATTGAAATGCAGCAGCGGCGGCACCACCCGTGCCGC
AGTGACAGAATTGCCTTGATCAGCCCGACGGTCCCCGCCGATGCCGTGCTGTGCCCCATGTTGCTCTTGGCCCGATCCAAGCGCGCAGGGGGTGCCGCGCCATACA
CCCGCGCCAGGCTGCGGTACTCAATCGGGTCGCCGATTGGCGTACCGGTGCCGTGCGCCTCCACCACACCGACCGTTTCGGGCTG
:::::::::::::::Rv30T7PEG.seq:::::::::::::::
CAACAGCGTTCCAGCGGCATACCACCGCACATGCCGTGCACCCGGCGCCGGGCGGAGTCGCCGCATAACACANGTACACCTTGGGAATCGGTGTGCGCCAGGGATT
CNACCGCGGGGTGGGGCCGGCGATCGCGCGCCAGGTCGAGTTGGCGCCGACCGTGATNTCACCGCGACGTAGTTGGCGTTGTGGTCCGCCATCCGCGCGGCGGGGC
ACGGCGCGGGCCGCCACCACGATGTCACGGAAGCCGGGGGCGAACGCTCGACGACCTGGTTACCGTCTCNGTCGCNTCNANCGTGGACCCGACNGCACGTGGGCAT
ATGTCCANAACGGACGNGGCCGGTTTCNTCGATGCNGCCGGGGTCCGCGACNTGCGGACNCNCNGNCACACCATCCGCCAGTCCGCGTGGCGTCCCGCCGCGACTC
TGCCTCGGCCGCGCCA
Clone Rv310
:::::::::::::::Rv310SP6.seq:::::::::::::::
CTCAAGCTTTGNCGACGATCGGGCGATGTCGATGANAGGAAACCCCAGCGCACAACCGACNATTTTGGCGTAGCCGGCGGACNTCTGCTCGATTCCGATCACGTCG
GCGCTCGCATCGAGCATGGCGCCGGCGACGGCTAGCAGCGATCCGCCGTCGTCGAGGAACACGACACGAGCCGTACGCCCGGCCGTAAGCCGCGCCCAGGATTCGG
CGAAAAACCGTTCTACGTGGCGGGTGTACTGGGTGTCCAATGATTCGTGGGGTGCGTAGGCGTCGCTGCAATCGTCGACATAAATGCCGTCGGCCCGCATCGCGTC
AACAACTCCCGGGTGAGTGGAATANCACTTGCCGA
:::::::::::::::Rv310T7.seq:::::::::::::::
TCCAACGCGGTGACAGATTTGTCTATCCTGGACCTGACGGTGAGGTCGAAGTTTTCCAGGAATTCGGCAAAATCGGTAAGAGCCTGAAGAATTCGGTATCGCCGGA
CGAAATCTGCGACGCATACGGGGCAGATACGCTTCGGGTTTACGAGATGTCGATGGGGCCGCTGGAGGCTTCACGTCCATGGGCCACAAAGGATGTTGTCGGCGCG
TACCGTTTTCTGCAGCGGGTGTGGCGCTTGGTCGTCGACGAGCACACCGGCGAAACTCGGGTGGCTGACGGCGTGGAACTCGACATCGATACGCTACGGGCGTTGC
ACCGCACCATCGTCGGCGTGTC
Clone Rv311
:::::::::::::::Rv311SP6.seq:::::::::::::::
CTCGTCCTTGACTACGCCCAGTATCGAAANCCTCCTGTGCCGGTNCGCTAAACACCCGGCGGACACTCANACGGTGCTGGTGGTGCGGCATGGCACCGCGGGCAGC
AAAGCGCACTTCTCCGGGGACGACAGCAAGCGACCGCTAGACAAGAGGGGTCGTGCGCAGGCAGAAGCGTTGGTACCACAGCTGCTGGCGTTCGGCGCCACCGATG
TTTATGCCGCCGACCGGGTGCGCTGCCACCABACBATGGAGCCACTCGCCGCGGAACTGAACGTGACCATACACAACGAGCCCBCCCTGACCGAAGAGTCCTACGC
CAACAACCCCAAACGCGGCCGACACCGAGTGCTGCAGATCTTCG
:::::::::::::::Rv311T7.seq:::::::::::::::
GTATCGCCTCCNCCTTTGGCCACCAGCAGCCACAGCGCGGTTCGCGGACCGAACGTGGACATCAATAGCCCGGAATCGGTGTGTGCAAGTTGGTAAACGGTGTTGA
TCCCAAGCTTTGCCAGCCTTTTCGTAGTCTTGGGCCCCACACCCCACAGTGCTTCGACGGTACGGTCACCCATGATGGCCATCCAGTTGGCATCGGTGAGCTGATA
GATGCCAGCTGGTTTCGCCAACCCGGTAGCGATCTTGGCGCGCTGCTTGTTGTCACTGATACCTATCGAGCAAGACAGCCCGGTTTGCGACAAGATGACTTTTCGG
ATCTCTTCNGCGAACTTCCAATGGGGGTCTCCGGANT
Clone Rv312
:::::::::::::::Rv312SP6.seq:::::::::::::::
CTCAAGCTTTTGGTCTAGCCGGCCGAGCACGATACGGGTGTCCTTGGCCACCGGCGGCGGCTGTCCGGGAAATGGCGGGTCCCCGGTGGTTTTGCTGANGANTGCT
GAACCGTAGTCGAAGTGGGCGGCGTCAGACTCCACCCAGCCAGCAGGCAGCGCGAAGCTGAATCCTCCAACCGGGTTGTCGATCCGGACAGGTTGGGTGCGTTTTG
GGGCAATGACAGGTGGCGGCGGTGCGTTCGGGTCGGCCGGCGGAGGTGCTGCGTTGGGATCNCCCGGCTGGGCATTCGGCNTTNTTGGCGGCGGCCGGTGGTGGGG
GGCAACANGTGTCCCGGTGCGGGTGGCGCTGC
:::::::::::::::Rv312T7.seq:::::::::::::::
ATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCCGCGGTACCCGCTCAACTTTCTCTCGACCCTCAACGCCATTGCGGCACCTA
CTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGC
ACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCT
ACGGCGACCCGGCCTATGGTTATTCGACCTCGCCGCC
Clone Rv313
:::::::::::::::Rv313SP6.seq:::::::::::::::
CTCAAGCTTGCAATGCGGGTCGGGATGCCCATGGTTGGAANATGGTCGCCCTGGCGTCNAATACGCGCGAGCGCATGAGCTCACCGGTTCGGAACAACGTATCGAA
AAACGTCGCACTGCTGGCAGATGGTATCTCCGATGTGGTTGTAATTTGTATCCCAACTCTAACTGTGCTATCGGATCAGCGTGAATATCGANATATTGCGAATGCG
ATGACAGGCCGCCATTCGGTTTATTCGCTTACGCTTCCCGGGTTCGATTCGTCTGATGCACTGCCGCAAAACGCGGATATGATTGTTGAAACCGTATCTAACGCAA
TTATTGATGTGGTAGGCGGCAGCTGCCGTTTTGTGCTGTCGG
:::::::::::::::Rv313T7.seq:::::::::::::::
CAAATACACGCCGGACGCACAGGCGGACATCGCCATCCCGAGCACACCCAAAACGGGATACAGGATGGAGGCCAACGCCACGGCCGCGCCCAGGATCACCAACCAC
ACCGGCTTGGTCAGCTTGTCGGCGGCGGTATAGGCATCGGGCCGCTGCAACGCAGCATGCACAAACGCGTACACCGCTGTCACCAAGACGGCGAGCAGCAATACCA
GCATGACGGTACCCACGAGGTGGCTCACGCATTCAGACTATGCGGTTTGCATCCAACACG
Clone Rv314
:::::::::::::::Rv314SP6.seq:::::::::::::::
CTCGTCCTTCGGCCTCGCTGCAGGAGTGGGAGCCGCAGGGCTGGAAATCCGAAAAACGAGCCGGTGATCGCACTGTCGCCGATCGGCGCCGCACCTGGTTGGTGTT
ACGGATGAATCCGCAGCGAAATGTGGCTGCGGTGGCGTGTCGTGACTCGTTGGCGTCGACGCTGGTGGCAGCCACCGAGCGGTTGGTCCAGGATCTGGATGGGCAA
AGTTGTGCGGCCCGGCCGGTGACGGCCGATGAGCTGACCGAGGTCGACAGCGCCGTGTTGGCTGACTTGGAACCGACATGGAGTCGCCCCGGTT
:::::::::::::::Rv314T7.seq:::::::::::::::
GTCTAGNCCGCCGAACACGATACGGGTGTCATTGGCCACCGGCGGCGGCTGTCCGGGAAATGCGCGGGTCCCCGGTGGTTTGCTGAAGANTGCTGAACCGTAGTCG
AAGTGGGCGGCGTCAGACTCCACCCAGCCAGCAGGCAGCGCGAAGCTGAATCCTCCAACCGGGTTGTCGATCCGGACAGGTTGGGGTGCGTTTGGGGCAATGACAG
GTGGCGGCGGTGCGTTCGGGTCGGCCGGCGGAAGTGCTGCGTTGGATCGCCCGGCTGGGCATTCGGCGTGTTGGCGGCGGCCGGTGG
Clone Rv315
:::::::::::::::Rv315SP6.seq:::::::::::::::
ATCAAGCTTGAGATTGGGCGTCAACGGGTGTCGGCACCGGCGTCCTGCAGTTGGTAGGCCTGCAGTTTGTGCATCAGGCCGATGCCGCGGCCCCTCGTGCCACGCA
TGTACANCACCACGCCGCGCCCCTCACGGGCGACCATCGCCAGCGCGGCGTCCAGCTGAGGCCCCGCAATCGCAGCGGCGTGACCAAACACATCGCCGGTCAAGCA
CTCCGAATGCACCCGGACCAGCACGTCG
TCACCGTCGGCGTTGGGCCCGGCGATCTCGCCGCGGACCAGCGCGACATGTTCCACGTCCTGTAAATGCTGGTGTANCCGATGGCGCGAAACTCCCCCATGACAAN
TCGGAATCCCGCGCCTCGGCGACCCCGCTCAATGTTGCTTCTCNTGCTTG
:::::::::::::::Rv315T7.seq:::::::::::::::
TCGACNAGCATTCTTGACNGTTGTTTTGGCTCGGCATGGTTAGCCAAGGTTCTGCGGTCCCACCAGATCATCTTGGTCCGGTAGCGCTCGTCCGGGTATGCTGCCG
CCGGGATTCTCGCTGCTATTACTCCCCCCGAAGAACGCCACCGGTCCAGCGCGTGGCCGCCGCGGTCCCCATCACAAACTGAACCCCCAACAGGGGGACATGCTTA
GCGGTAGGGCGCGCGCCAAGGCGGCAGCAATCGCATCACTGCGCTGCGCGTCACTATTAACCCAGCCGGACTTCACTTCCACGACCCCCGAATGGCGCCGGTCATT
GATCATCTTGCGCACCCGCGGATAATCCGGGAT
TG
Clone Rv316
:::::::::::::::Rv316SP6.seq:::::::::::::::
ACCGGGGCCACTCCGCACAATCTGTACCCGACCAANATCTACACCATCGAATACGACGGCGTCGCCGACTTTCCGGCGGTACCCGCTCAACTTTGTTCNACCCTCA
ACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCNGCGGTTCCGCTGACCAATACGGTCGGTCCCACNATGAC
CCANTACTACATCATTCGCACGGANAACCTGCCGCTGCTAAAGCCACTGCGATCGGTGCCGATCGTGGGAACCCACTGGCGAACCTGGTTCAACCCAAACTTGAAG
GTNATTGTTNACCTGGGCTACGGCGANCCGGCCTNTGGTTATTCCACCTCNCCGCCCAATGTTTGCNACTCCCGTTCGGGGTTGTTCCCNAAGGTCAAACCC
:::::::::::::::Rv316T7.seq:::::::::::::::
CGCTCAAGCGCNTGAGGCCGAANCGGCTGGTTACGACTCCCTGTTTGTGATGGACCACTTCTACCAACTGCCCATGTTGGGGACGCCCGACCAGCCGATGCTGGAG
GCCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGAGCGGCTGCAACTGGGCGCGTTGGTGACCGGCAATACCTACCGCAGCCCGACCCTGCTGGCAAAGATCA
TCACCACGCTCGACGTGGTTAGCGCCGGTCGAGCGATCCTCGGCATTGGAGCGGTTGGTTTGANCTGGAACACCGCCAGCTCGGCTTCGAGTTCCGGCACTTTCAG
TGACCGGTTC
Clone Rv317
:::::::::::::::Rv317SP6.seq:::::::::::::::
CTCAAGCTTGCGTTCGATGAAGTAGTCGTCGGTCAGCGCCGCCTCTTCGAGCTCCTTGGCGATGCCCAGCAAGGAGTCATCGCCGCCGAGCTTGGCCAGGATCTTG
TCGGCCTGTTCCTTGACGATGCGGGCCCGCGGATCGTAGTTCTTGTAGACACGATGACCGAAACCCATCAATTTGACCCCGGCCTCGCGGTTCTTGACCTTGCGTA
CAAACTCGCTGACGTCGTCGCCGCTGTCGCGAATGCCCTCGAGCATCTCCAGGACAGCCTGATTGGCGCCGCCATGAAGCCGGACCCCATAGTGCGTTGATGCC
:::::::::::::::Rv317T7.seq:::::::::::::::
GGTCAGGCCCGAGCAGGCGCGAGGAACGACGAACCCAACAAGCCATGGTGGTTGGCGCCGTCGAGAGGTCGGCGGTCGCACAACGGGAAGATCGCCTTGAGCGTCG
CTCGACCGCCGCCTCGAGTTGGGTCATAACGAAGTAGCTGATGCCGATCATGTCGACGTTTCCGTCGCATCAGCGTGCAGCGGCGACCCACTCNACCGAGGTCTCG
TGCCGCCGCGGCCAGGGCACCAGCAGTGACGAGTCCAGGCCGCCGTCGGGCCAAGCAGTCGCGGTGCCANCCGTGGTGGGTCCGGCGATGGTTGGTGTGCTCATTT
CGGGAACGCCA
Clone Rv318
:::::::::::::::Rv318SP6.seq:::::::::::::::
CTCGAAGCTTTAACAGCATCAACCCCGCCCCGCACCAGCACCGACACNATGTCGATGCCATCGAAGGTGAATGTCGAACTGGCGCAAACCATCGCGACCGCGACCA
CCGGCAACATGGGTACCGGCGATTTCCGGTGCCAATGCCGACCCGACGGGCCGCTCTCACCGCAGGTGACCTCGATCACCGAGACCANCCGGCCGTTNTNNTCACG
CACCCCTACCGTGTCACGCCCAAAACGGCGCTGGTGGTCGATTGCCGGAGTGCACCCCNCACCCCAGTGTGCGTCCCCGGATCC
:::::::::::::::Rv318T7.seq:::::::::::::::
TGATGCCGCACCCGATCGACGGTCGTTGGTCGGGGTTGACTGGCGCCCGGCGAAGCAGGGCGTCGACCGCGGCCCGGACGTCGGCGGCCGTCACCGGGTCGGCCAT
TGCCCGGGCGGGAGTCGTCGAGCTGACCACGGTAGACAAGTCGGCGCTGGCCGTCGAAGACNAACGTGCGGGTGTGCAGGCCGCGGAGAAGGCGCCGGCGACNTTC
TTGGGTTTCGTCGTANAGATACGGGAACGTCCAGCCGTGGCGGCGGGCCTCGGCGACCATCTGATCGGCCCGTCC
Clone Rv319
:::::::::::::::Rv319SP6.seq:::::::::::::::
TTTCGGGCGAGGCGGTATANCTTCCCNTCGTACCGGCGACCGCCAGCCGANAAGCTCGTTTTCCCAGTGTTGCTGGGGATTCTCACGCTGCTGCTGANTGCGTGCC
AAACCGCTTCCGCTTCGGGTTACAACGAGCCGCGGGGCTACNATCGTGCGACGCTGAAGTTGGTGTTCTCCATGGACTTGGGGATGTGCCTGAACCGGTTCACCTA
CNACTCCAAGCTGGCGCCGTCTCGTCCGCAGGTCGTTGCTTGCGATAGCCGGGAGGCCCGGATCCGCAATGACGGATTCCNTGCCANCGCTCCGAGTTGCNTGCGG
ATCGACTACNAATTGATCACCCANAACCATCGGGCGTNTTACTGCCTGAAGTACCTGGTGCGGGTCGGATACTGCTATCCGGCGGTGACAACCCCGGCAAGC
:::::::::::::::Rv319T7.seq:::::::::::::::
GTTTTGGCTCGGCATGGTTAGCCAAGGTTCTGCGGTCCCACCAGTCATCTTGGTCCGGTAGCGCTCGTCCGGGATATGCTGCCGCCGGGATTCTCGCTGCTATTAC
TCCCCCCCGAAGAACGCCACCGGTCCAGCGCGTGGGCCGCCGCGGTCCCCATCACAAACTGAACCCCCCAACAGGACATGCTTAGCGGTAGGCGCGCGCCAAGGCG
GCAGCAATCGCATCACTGCGCTGCGCGTCACTATTAACCCACCCGGACTTCACTTCCACGACCCCGAATGGCGCCCGGTCATTGATCATCTTGCGCACCGCGGATA
ATCCGGGATTGCCAGCCCATTCNACTACCGCATGCGAGTCATCGGCTGACCGCAGCGGTC
Clone Rv31
:::::::::::::::Rv31SP6.seq:::::::::::::::
TCGCCTAGGCGGGCTTCCCCTTCCGTCCGAGCNGTCAGAAGCTCCTATGACAATGCACTACCCGAGACNATCAACGGCCTATGCAATACCNAGCTGATCAAACCCG
GCAAGCCCTGGCGGTCCATCGAGGATGTCGAGTTGGCCACCGCGCGCTGGGTCGACTGGTTCAACCATCGCCGCCTCTACCGGTACTGCGGCGACATCCCGCCGGT
CTAACTCGACGCCGCCTCACTACGCTCAACGCCAGAGACCANCCGCCGGCTGACGTCTCAGATCAGAGAGTCTCCGGACTCACCGGGGCGGTTCATCCCCACTGTC
GATAGCGTCTGTGGATAACTTTGTCTGCA
:::::::::::::::Rv31T7.seq:::::::::::::::
GCGCGTNGAACTGATAGGTGCGGCCCGGCTCGAGCANGCCGGCCATTTGTTCGATGCGGTTACCGAAGATCTCTTCGGTGACCTGCCGCCCGCCGGCCAGCTCGGC
CCAGTGCCCGGCGTTGGCCGCCGCGGCGACAATCTTGGCGTCCACGGTGGTCTGGGTCA
Clone Rv321
:::::::::::::::Rv321SP6.seq:::::::::::::::
CTCAAGCTTCAATACAGAGTTATAAACTGTGATAATCAACCCTCATCAATGATGACNAACTAACCCCCGATATCAGGTCACATGACGAAGGGAAAGAGAAGGAAAT
CAACTGTGACAAACTGCCCTCAAATTTGGCTTCCTTAAAAATTACAGTTCAAAAAGTATGAGAAAATCCATGCAGGCTGAAGGAAACAGCAATAACTGTGACAAAT
TACCCTCAGTAGGTCAGAACAAATGTGACGAACCACCCTCAAATCTGTGACAGATACCCTCAGCTATCCTGTCGTCATGGAAGTGATATCGCCGGAAAGGAAAAT
Clone RV322
:::::::::::::::Rv322SP6.seq:::::::::::::::
CTCAAGCTTCGATCGACATTACTCCCGCCTTGGGTCTGGTCTCCGAGCTGGTCGGTCATGGTCGGACCTGCTGGTAGTGGGGATCTAACGCAACATGGTCGGGATT
CATCATGGTGTACCCGTGATACCCATTCGCAGCTGCCGGTGAACCCCCGCGATGCCGGGATTTCCAGCCGCACTAGGATGTCTAGCCGGCCAGCCGCTGCCGCCGG
ACTTCGGGATGTTCGGTATACCANCGATCGGCAATCTTGCGTATCCGCCGATGCTCGAACGCTANCCACGCCAAACCAACCACTGTGACNACAATCGCCACCACAC
CAAAGGTCATGCCCTCGGCGTGATGTCCGGTGCCGAAAGCCGCAAGAGCTCCGACGCCGCC
:::::::::::::::Rv322T7.seq:::::::::::::::
CATTCCCAATTGAATTTCCCNATCCCACAATCTCGGTTCAGATACAGGTCGCCATACCCCTTACTTCGGCAACGCTGGGCGGATTGGCCCTGCCGCTGCAGCANAC
CATCGACGCCATCGAATTGCCGGCAATCTCGTTCAGCCAATCCATACCCATCGACATTCCGCCGATCGACATCCCGGCCTCCACTATCAACGGAATTTCGATGTCG
GAGGTCGTGCCGATCGATGTGTCCGTCGACATTCCGGCGGTCACCATCACCGGCACCAGGATCGACCCGATTCCGCTGAACTTCGACGTTCTCAGCAGCGCCGGAC
CCATCAACATCTCGATCATCGACATTCCGGCGCTGCCGGGCTTTGGCAACTCGACCGAGCTGCCGTCGTCGGGCTTCTTCAACACCGGCGGCGGTGGCGGCT
Clone Rv327
:::::::::::::::Rv327SP6.seq:::::::::::::::
CTCAAGCTTTCGGCGGAGACGGACANNTTGCGAACATTGATGACAAAATAGAAATCATTGATGGTTTGAGTCACCAGGCCGATCAAGCCTTCGCCGAGCCAAATTC
CAATCAAGAGGCCCAAGCCCGTACCAATCAGCCCGGCAACGAGGGATTCCGTCATTATCAGCCAAAATAACTGCTCTCGGGTTACACCCAAACAGCGCAATATGGC
GAAAAACGGTCGCCGTTGCAGCACATTAAATGTCACGGTATTG
:::::::::::::::Rv327T7.seq:::::::::::::::
AGCTTAACTGCTCCCTAATACCTGGGGCTGTGCCTGCGGTGTATGCACGGCATACGGACATCCNTCCCCTGAGACCCNCGGTCTAATCAGCCACGTGTCCACCATC
AGGGGTCAACCCCGGCCAAGGGCGACGGCACCCCAAGTTCGCCGACCGTTAACCTATTGCTGTGAGCTTCATTTGCTGCGAGCAAAACAGTTGGTCGGCCGTTAGG
AACTGAATTGACACTCAACCGATTTGGTGCCNCCGTAGGTGTCCTGGCTGCGGGTGCGCTGGTGTTGTCCGCGTGTGGTAACGACCACAATGTGACCGGGGGAGGT
GCAACCACTGGCCACGCGTCCGCGAATGCTATTGCGGGGG
Clone Rv328
:::::::::::::::Rv328SP6.seq:::::::::::::::
CTCAAGCTTGGGGTGGCGCTGTCGGTCGGTGTGCTTGGCGGCGTCGGTATCAACACCGCCCACGAAATGGGCACAAGAAGGATTCGCTGGAGCGGGTGGCTGTCCA
AAATCACCCTCGCCCAGACCTGCTACGGGCACTTCTACATCGAGCACAACCGTGGCCATCACGTCCGGGTGTCCACACCGGAGGACCCGGCGTCGGCGCGGTTCGG
CGAAACGTTGTGGGAGTTCCTGCCCCGCAGTGTTATCGGCGGCTTGCGCTCGGCCGTTCATTTGGAGGCCCAACGGCTGCGTCGGCTCGGCGTCAGCCCCCT
:::::::::::::::Rv328T7.seq:::::::::::::::
GCACCAAGGCCCCACACGTCACCCTGTGACCTCCTGCGCCGACCCGCCCCGAGGTCCTGGCCGTTACAACCTGAACGGGCGAGCCCGGAGTCTGGTACGCATCGAA
CAAAGAGCAAGGTGCATGGGCGGAGTTGTTCCGCCACTTCGTCGATGACGGGGTCNATCCATTCGAGGTCCGTCGCCGCGTCGGTCGAGTGGCGGTCACACTCCAG
GTACTCGACCTCACAGACGAGAGGACTCGATCCCATCTAGGTGTGGACGAAAACAGTCTTCTGTCCGA
Clone Rv329
:::::::::::::::Rv329ST6.seq:::::::::::::::
TCGCCTCCGCATATGGGTCGACGCCAAGCGGGTCCGGATTTTCTGGGCTTCATCGCTCGCGCCGTCGCGACAAACAGCGCGGTCGACCGACACTCGTTGTGATGTC
CCAGCTATCACCTTCGGTACGCACCCATCGACCCTACNCGGCTATCTCAGCCGCGATCTCCAGGCTCCGCCGACGGAGGTGCATCCCGGTCCGGATCCCCACTAAC
CCGGCACCATTGGCGTCN
:::::::::::::::Rv329T7.seq:::::::::::::::
GTCCTCGAGTGCCGCCGTCGNCACNCCCAGCGCCCGCGCGGCCACTTGGATGCGACCCGTTTCAAGTCCCTTCATCATCTGCGAAAAGCCTTGACCCATGGCTCCG
CCCAGGATCGCCGAGACCGGCACCCGGAGGTTGTCGAACGACAGCTCGCAGGATTCGACGCCCTTGTAACCCAACTTCGGCAAGTCCCGCGACACCGTGAGTCCCG
GCCCGGGTTCGACGAGCACGATCGACATGCCTTGGTGCCGCGGTGTGGCGTTCGGGTCGG
Clone Rv32
:::::::::::::::Rv32SP6.seq:::::::::::::::
GGCATACCAATGTGGACTTCTGCTCACCCACGATATCCGTGGTCTGATCCGCTGCTGCGGCGGGCTGCNACCTGCNTTCTCNGCGGCACCCGTNACTACATGCNCG
CGCCGCACGCATACGTCGCGGCGGGACCCACTCCNACTGGTCGACGGTGCTGGCCGCGTGTCCGCANGTCCCNAACCCGGCCGCACCCGACGAAACCGGCCGCGTC
CGTTCTGGAGGCCAACGCTCATGTGCCGTCGGGTCCATGCTCGACGCCATCGAGACCGTAACCAGCGTCCTCGAGCGGTCGCCTCCGGCTTCCGTGACATCTTCGT
GGCTGCTCGCGCCGTGCCGCCGCGCGGATGGTCGACCACAACGCCAACCACCTCGGCGGTGACATCACCGTCCGCGCCACTCGACCTGCGCGCCGATCGCGGCCC
:::::::::::::::Rv32T7.seq:::::::::::::::
GTGAGCAGACCTACGCCNCCTGGTTGCGCCAACTCGGTACCGATCATGGCGCGCNGCCTGTCGTCACCGATACCCAGCGAACAAGACAGCCCGGTCCGCGACAAGA
TGACTTTCCCGATCTCTTCGGCGACTTCCATGGGGTCGTCCGGAAGTCCCGGGCGCCACCGCGAGGTAACCCTCGTCTCAGTCCCATACGCGACCGGGTATCACGT
CGCGCAACAACGCCACCACCTCCCAGACGCCNCGTTGTACGCGGCTGGGTTCCACNGCAATAAGTGGCCTCANGGCATCGTCCGGCGGCGGTCCNCAAACGCA
Clone Rv330
:::::::::::::::Rv330SP6.seq:::::::::::::::
CTCAAGCTTGAGGTTAACTTTGAACGGATCGAGCTGGACGTTCGAGACGGTGATCGGGCCGAACCTGAATTGTCCGGTAATGCCCAACGCAAAAAGCAGGGTGGTG
GCCGGGGCGGTGAAACCGGCGTCGGCGGCACCGTCGAAATCTATGTGGATTGCCGGAATGGGATGTCCGGCACACGGCGAAACCGTAGTTCGCTTGTCCCGTGAGC
CCAGGTGGATGGGGGAAAGATCCTGGTGTCCGGGATAATAATGGGCCGATGCCGCCGGTTGAAGCTCCACTGGATCGGGAATTCCGGAATCTTGATCCGACGCTTC
AGGCCGAACAGGCCCCTC
:::::::::::::::Rv330T7.seq:::::::::::::::
CGGCGACGTCGCGATACGCCGAGCAGTTGGGAATCGCTCTGCAGCAAACCAATATTCTGCGCGACGTTCGAGAGGACTTTTTGAATGGACGGATCTACCTGCCGCG
CGACGAGCTGGACCGATTAGGCGTACGCCTCCGCCTGGACGACACCGGGGCACTCGATGACCCCGACGGACGGCTCGCGGCNCTGCTGCGGTTCAGTGCCGACCGC
GCCGCAGACTGGTNTTCGCTGGGACTGCGGCTGATTCCACACCTCGACCGCCGCAGCGCTGCCTGCTGTGCGGCCATGTCTGGCATCTACCGCCGTCAGCTCGCCT
TGATCAGAGCATCGCCGGCGGTCGTCTA
Clone Rv331
:::::::::::::::Rv331SP6.seq:::::::::::::::
CTATAAAATACTCAAGCTTGATGCCGCCGAAACCGAGCGTGAGCACGCCGCCAGGCACCAGGCGCGGGTCCGGGGCCGGCCCCCCCCCGGCCCCCTGCTCCGCTCG
GTGATGGCACGCCACCGCGACACCACCCGGNTGCGCTACGTCNAGCCATACCGGGCGGAGCTACATCGGCTCGGCCGCCCAGTGTTCGGGCCTCTTTCGAAGGTCN
AGGTCNATACCGATTTGCGCATCCGCAGCCGCACCCTGAACNACANAACCGTGCCCTACTATTGCTTGTCNGGCGGGGCCAAAAAACAGCTTGGCATCCTGGCCCN
ATTGGCCGGCGCGG
:::::::::::::::Rv331T7.seq:::::::::::::::
CTTCGGTCGCAGTGTGCGAGTGATAGATGACGACCGGGACCTCGTCGGCATCTTCCATAGCCCCGCACACCTTCAGTTGCTCACCGGAATCCAACCGGTAGAAGGT
CGGCGAGCGCTCGGCATTGGTCATCGGGATATGCCGCTCGGGACGGTCAGAGCCCTCGGGTCGGCCAGCACTCCGCAGCGCTTCGTCGGGGTGGTCCCCGAGCCCA
TGGCCACCATCGCATTCACCAGGTCTGCGCGAATCNCCANCACGTANACNGTTCCTTTCCTAA
Clone Rv333
:::::::::::::::Rv333SP6.seq:::::::::::::::
CTGGCACCAAGGCCCCACACGTCACCCTGTGACCTCCTGCGCCGACCCCGCCCCGAGGTCCTGGCCGTTACCACCGAACGGGCGAGCCGGAGTCTGGTNCGCATCG
AACAAANAGCAAGGTGCATGGGCGGAGTTGTTCCGCCACTTCGTCGATGACGGGGTCNATCCATTCGAGGTCCGTCGCCGCGTCGGTCNAGTGGCGGTCACACTCC
AGTACTCGACCTCACAGACNAAAGGACTCNATCCCATCTAGGTGTGGACNAAACAGTCTTCTGTCCGACNACTACACCACCACCCAGGCCATCGCCGCCGCCCGGG
CGATGCCAACTTCGACGCCGTACTGGCCCCGGCCGGGGGGTCCCCGGTTGTCAAACACTTGCCGTGTTCNTTCACGCNCTGCCCCACATCCAACCCCCAACG
Clone Rv334
:::::::::::::::Rv334T7.seq:::::::::::::::
GTTCTTGGCCCATGCGGAGGTATCGCCGTTTCCACCACGCGGTCGGGGTGGCGTTGCATTAGCTCACCGATGGTGCGCTTGTGCAGGCCGCCGGGATACCCCCGAG
TGCCGGTAAACCATCTTGTGCTGC
Clone Rv335
:::::::::::::::Rv335SP6.seq:::::::::::::::
CAATACTCAAGCTTGGCGTGCCGTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACNACNACGTCGTCCGCGGGACACACCT
CGATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCCGGAACGTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGG
ATC
:::::::::::::::Rv335T7.seq:::::::::::::::
CNTCATGATGATCATCACCCGAAGTGTGGTAGCCGCAGTGGTTATCGTGGGTACCGTCGTGCTTTCCATGGCGCCTCTTTCGGGCTTTCCGTATTGGCTCGGGGAG
CACATTCTGGGTATCGAGTTGTACTGGATGGTGTTGGCGATGTCGGTGATCCTGCTCCTGGCGGTGGGATCCGACTACAATCTGCTGCTGATTCCCCGGTTGAAAG
AGGAAATTGGGCCGGATTGAACACCGGAATTATCCGTGCCATGGCTGGTACCGGGGGAGTGGTGACGGCTGCCGGCATGGTGTTCGCCGTTAGCATGTCGTTGTTT
TGTGTTCAGCGATTTGCGAATTATTGGTCAGATCGGTACCAC
Clone Rv336
:::::::::::::::Rv336SP6.seq:::::::::::::::
ATACTCAAGCTTTTACGGTGATCGCNCATCACCTGGTTCATGAACTGGAAGCAGCCCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCG
GGTGCAGGTGCTCGGGCAGCTCGGCCGCNACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGANNAACGACGCCAGTCCGCTACGTNACCCCTCCCGGAC
TGTCCATGGAGAACAGCGCGTTCTCCACCGACCGGGCCCGGGTGTGGGGTNTT
:::::::::::::::Rv336T7.seq:::::::::::::::
GCTGGTAGAGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGCGCAGGCTATCGCACCCGTTATCGGCTAC
GAGCAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGGCGTCAAGCATGTGTGCCCCCAAT
TATCAGGACTGACCTCCGGCTGACCGGCATGTTTGGTCGCGATGCCTGGGCG
Clone Rv337
:::::::::::::::Rv337SP6.seq:::::::::::::::
GCTTTCCGCCGATACCCGCCATGTCNCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGGATCCCAAGTGCGGATGATCGGGCCCCCGTACGTCGTG
GTGTACCTCGTCGGTAACAACGAAACCGAAGCGTATGACTCGGTCCACGCGGTGCGGCACATGGTGGACACCACACCGGCCACCGCACGGGGTGAGGGCTATGTCA
CCGGTCCGGCANCACTCAATGCCGACCAGGCCGAGGCCGGANACAAAAANTATCGCTAAGGTCACCGGATCACNAGCATGGTGATCGCAGCAATGTTGCTAGTGAT
CTACGCTCCGTAATTA
:::::::::::::::Rv3337T7.seq:::::::::::::::
CTTCCAACCCGAATTGGCTTTCGGCGCCATCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACAACCTCGATGCTGCCGCCATGGACCG
GTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGAGC
GCATCGCCACCGGAGCGACGGCCAAGGCGGCGTGCCANGTCGCCCGGCGCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCCCA
Clone Rv338
:::::::::::::::Rv338SP6.seq:::::::::::::::
TACTCAAGCTTCGCGAGATCCGGATGGCACTCACGCTGGACAAGACCTTCACAAAATCTGAAATCCTGACCCGATACTTGAACCTGGTCTCGTTCGGCAATAACTC
GTTCGGCGTGCAGGACGCGGCGCAAACGTNCTTCGGCATCAACGCGTCCGANCTGAATTGGCAGCAAGCGGCGCTGCTGGCCGGCATGGTGCAATCNACCAGCACG
CTCAACCCGTA
:::::::::::::::Rv338T7.seq:::::::::::::::
CCCACGACTTTCTCCTCGATCAGTTGGATTTGTACGAAGAGGCAACGAAAGCAGTGATCCTCGGGATGGTCGACGCCTACATCGACCCCCGTTCGCCGCCGCACAG
CCTGCTAGATGCGCTGGGCGAGCAGGTCCCACAGTTCGCCGCTAAGGCACGGCGTCTGTTCCCGTCCGGATCGCCATTCGGCCTCGGCGTCCTGCTCCCATTGGAT
CAATAGGGCTGGCAGCTCCGTCGGCAGGGGCCTACGCCTCACCCCGTCACG
Clone Rv339
:::::::::::::::Rv339SP6.seq:::::::::::::::
CTCAAGCTTATGCGCGCCGGCCGAGGTCTGCTCACGGCAACCCCTGAAGTTTAGGGGACNACCTACTCAGCGCAAAATTTCGCTAATGTGAGTCCGGCCCACCAGG
GGNANATCACCCATGTCGATCATGATCTACCCCGGATACCGGATTGGCGGTAGCGCCCACGATCGTCNAAATNTCCGCCTGAATCATCGGATAGCTGATCGGGGGT
CACGCGTTTTGANTTCACCGCGAACAGCCGCCAGCCGGCCCCGCANCGANCCGATCTCNTGCCCGGCCATGGGCCCCCAATCTTNTCG
:::::::::::::::Rv339T7.seq:::::::::::::::
GTGTGTGGTGGAACCCATCTGAGCAGTGTGCCAAACCGGGGCAGACAGCTCCCAATTGACGTGAGCCCCGCTCACTTGCTGGGTAAGCGTC
Clone Rv33
:::::::::::::::Rv33SP6.seq:::::::::::::::
CTTTACACTTCCTGCATCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTG
ACACTATAGAATACTCAAGCTTGGCGTGACGGCCACCGGGCCACTCCGCACCATCTGTACCGACCCAAGATCTAC
:::::::::::::::Rv33T7.seq:::::::::::::::
CAGGCATGCAAGCTTTAGCTGCCCGAATGCGTCACCCCGATGCGCCCAGATCGGGGCTTCGCAGATAAAGCACGAACAGGCGGGCAAAACGTCNATCTCGGAGCCG
GAAGGGCAATCAGCCGACCGTCGACGAACGACACCGGCGAGACCACTTAGGCAGTGACGGCCGGCCCGAACATTACGCGCTCGTTGATTAGGCGTTCGGTCTCGTC
CGCGGTCATGCCGAGCAGCTTGCGGCAGATCTGAACGCTGTCCTGTCCGGGCAGCGGCGCCGGGCGTTGGGTGCCTGCCCGAATGTGACGAAACCCGGAGCCGACC
CGTCTCGGCGGGCCGCGGACGGCGATCCGC
Clone Rv340
:::::::::::::::Rv340SP6.seq:::::::::::::::
CNCAAGCTTGCGGATGTTACCCCTGACAGCCTGAACTATGTCNAAAACACACGGCACCCGGAACGGTGTTGGGGACCCCATCGANTTCGAGTCGCTGCCGGCACTT
ATGGCCTGGGTAAAGGCCAGGCNANAGCCCGTGCGCATTGGGGTCGGTCAAAACCAACATCGGCCACCTGGAGGCGGCCGCCGGTGTGGCTGGATNCATCAAGCGC
GGTGCTGGCGGTGCAACGTGGGCACATTCCCCGCAACTTGCACTTCACCCGGTGGAACCCGGCCATCNACGCGTCGGCNACGCGGCTGTTCGTGCCNACCNAAAAC
CCCCCGTGGCCGGCGGC
:::::::::::::::Rv340T7.seq:::::::::::::::
GGAACCGGTAACCAGATCAGCTCGTCGACCTCACTGCCGGGGGTGAATTCCCCACCGGTGCTGCGCGTGCCCAGTAGTGCACCTTCTTGACGCCTCGAAAAGGGGG
AGTCGGTCGGGTAGGTCACCGTCAGGAGCCGCCTACCCAGGTTGGCGCNATAGCCGGTCTCCTCGAGTATCTCCCGCACCGCCCCCAGCCGTGCGGTCTCACCCAN
ATCCACTTTGCCCTTGGCAGCGACCAGTCGTCGTANCNGGGGCGGTGAATGACAACGATCTCGACCGGCCCTTCCN
Clone Rv341
:::::::::::::::Rv341SP6.seq:::::::::::::::
TACTCAAGCTTCAGAACAGGCCTGTTGTGGGCNCACCCGGCTCGCCGAGTTCTGCACGCACCGGCCTCAAGTGCGGCCCGCACCGCCGGCATCTCCCGGTCACCAG
GGCCGGGCCCGCGCCGCAGCGACGGGCGTGTTCGCGCAGTTCGCCGTCAATGATGCTGACCTGATCGGCCACCCGGGCGTTCTCGGCGTCGTCGCGTTCACTAATC
GCGGTGCTCAGCAGCGTCTCGACAGCCACCACCCGAGTGGCGACCAGCTGC
:::::::::::::::Rv341T7.seq:::::::::::::::
TAATGTCTTGCCAACGTCACCACAATCGCGATGAATTCAATCATGCCGCCCAGGGCGGCCAACCCAATGGTGGCCGCGAGCGGCAGCTCGATCGCAGCGGGGAGGT
TGCCGGCCGCCAGTTGATTCACGAACAGGGTGAGGTCATAGGCGGGCAGGATAGTGACGAAGGCAAGACCTATATCTGCCGTCGGAAGAAGAATCGAGTAGCCGGT
CGACACAACGGAAGCGAAAGTGTCCGCGATGTTGATGAGCGTGCCGGTTGTGGCGGCGGTGGCGGC
Clone Rv343
:::::::::::::::Rv343SP6.seq:::::::::::::::
TACTCAAGCTTTCGTCAGTTCATCGCGCCAGCAGCCAACAAGAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGCC
GCGAACGACGCCAGCGACCACATTCAGCANATGGCCAGCGCGTGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCGTGATCGACATCGTCA
CCGCCGCACCACTGCCGGCCTCGGGTTCACGCAGCCGTTGCCGCCCGCAGCGGANNATCACATCGCCGCGATCGCCCTGTTC
:::::::::::::::Rv343T7.seq:::::::::::::::
CCACCCGTGTAATTTGGGATGGGCNAAAAGGCNAAGCACCGCGTGGCCACGAACGCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGGAAGGCCGAAC
GTACGGCGTTTCAACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGGTAGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCCGCGG
TAGTCGCCGCCCGGGCGGCTACAGTCTGAAACGCGATGACCATCGATGTGTGGATGCAGCATCCGACG
Clone Rv344
:::::::::::::::Rv344SP6.seq:::::::::::::::
TCAAGCTTTAGCTGCCCGAATCCGTCANCCCGATGCNCCCAGATCGGGGCTTCGCANATAAAGCACNAACAGGCGGGCAAAACGTCNATCTCGGAGCCGGAAGGGC
AATCANCCGACCGTCNACAAACGACACCGGCGANACCACTTAGGCAGTGACGGCCGGCCCCGAACATTACNCGCTCGTTGATTAGGCGTTCGGTCTCGTCCGCCTC
ATGCCGAGCAGCTTGCGGCANATCTGAACGCTGTCCTGCCGGGCAGCGGCGCCGGGCGTTGGGGTGCCTGCCGGAATGTGACNAAACGGAGCCGACCCCCTCTCGG
CG
:::::::::::::::Rv344T7.seq:::::::::::::::
CCGGGGCCACTCCGCACAATCNGTACCNNACCAANATCTACACCATCGGAATACGACGGCGTCGCCGANTTTGCGGGTACCCGCTCAACTTTGTGTCGACCCTCAA
CGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGAACAAATNGACGCNTCGGTTCCGCTGACCAATACGGTCGGTCCC
Clone Rv346
:::::::::::::::Rv346SP6.seq:::::::::::::::
NCTGGCCTTTGGTCCACACTAANACAATACTCAAGCTTCCCGGCCGCAGAGCCGCCAACTCACGATATCGTTAACCGATATCCCGAGCCGATAGCTGCCGGCTCGG
GTGGTGGCCAGCGGCGCTGCGACNAAAGGTGTGACCGTCATGAACAGACACCACCGGCCGGCCGTCGGCCGTCGTCACCTGCTCGANATCTCAGCATCCGCAGCCG
GTGTGATCGCGCTTTCGGCGTGTNGTGGGTCNCCGCCCGAGCCCGGCAAGGCCGGCCCCGACACAACCCCGGAAC
:::::::::::::::Rv346T7.seq:::::::::::::::
CATCTGCCCACCACACGGACCGCGGTGCGGACGCGGCTGACGCGCCTGGTGGTCAGCATCGTGGCCGGTCTGCTGTTTGTATGCCAGCTTCCCGCGCGCAACTGCT
GGTGGGCGGCGGTGGTTGCGCTCGCATTGCTGGCCTGGGTGCTGACCCACCGCGCGACGACACCCGGTGGGTGGCTGGGCTACGGCCTGCTATTCGGGCTGGTGTT
CTACGTCTCGTTGTTGCCGTGGATCGGCGAAGCTGGTGGCCCCGGGCCCTGGTTGGCACT
Clone Rv 347
:::::::::::::::Rv347SP6.seq:::::::::::::::
GACAATACTCAAGCTTGACTGGCCACCCACCGGCATGACCACCCGACAGGCCCGACTGGTCGTACCACTCGAACGCCGGGGTGTTGATGTCCCAGCCGTGAANTCG
TCCTGCGCGCGCAGGCCGTCNAACAGGTACAGGGCGGGCGAATTGGCACCACCACTTTGGAATTGGACCTTGATGTCACGGCCCATCGACGGCGACGGCACCTGCA
GGTACTCCACCGGCAAGCCCGGCCGGGAAAATGCCCCGCGGTCNCCGTGCCACCGACGGCGCCGANCAAACCCGACACTAGGGCCGCGCCNACGGCCCCCGACCAC
NANTCNACGCGACATACCCGTGACGGCGCCACNAACCCTGTCAACA
:::::::::::::::Rv347T.seq:::::::::::::::
CCTCCAACTCGGCGGGGAAGCGACNCCAGCCTACCGAGCTTGGAGTCCANGACGCCAGCGGCGGCGTCGGTCGCGTCGTGGTGCCGCCGGCGGTGGCGTTGGCTGG
CAACGATCTCCACCCAGCCGGTCGGGTTACCCACGATCTCGGCATANACGCGGGCCGAGGCCCGGTGCGATACCGTATTGCGTCAATTGGGACCGGTTGTGCATTC
GCTAGCTCGGTTGCCACACCCGTCAGGGGTTCGACGTTGGCGGGTTCGGCGGCCCCCANCACCGCTGTCACCATGCCCGCCAAGCCGACCTGCGGCGCCCACCAAC
TGCAGCACCANCATGTCGCCGTCGCGCGCCGCGATCACATGG
Clone Rv348
:::::::::::::::Rv348SP6.seq:::::::::::::::
CTCAAGCTTTTTGAGCGTCGCGCGGGGCANCTTCGCCGGCAATTCTACTANCGAGAANTTCTGGCCCGATACGGATCTGACCGAANTTCGCTGCGGTGCANCCACC
TCATTGGCGATGGCGCCGACNATGGCGCCTGGACCGATCTTGTGCCGCTTGCCGACGGCGACGCGGTAGGTGGTCAAGTCCGGTCTACGCTTGGGCCTTTGCGGAC
GGTCCCGACGCTGGTCGCGGTTGCGCCGCNAAAGCGGCGGGTCGGGTGCCATCAGGAATGCCTCNCCGCCGCGGCACTGCACGGCCAGTGCCGCGGCGA
:::::::::::::::Rv348T7.seq:::::::::::::::
CNCCAGCTTGATTGGTCTGGTTGCATTGGCCAGCTGCGCGAGCCTGGCTCACTTCAACTACGACGACCGCAAAACAATTGCCGCCTTCGGATCCGAGTTCGGTTGG
TACGCGGCAATGGAGCACCATTTCTCGGTGAATCAGACTATTCCTGAGTACTTGATCATCCACTCTGCACACCGACCTGCGAACCCCGCGCCCCTTGCCGACCTGG
AGCAGCTGGCGCAACGTGTGAGCCAGTCCCAGGCGTTGCCATGGTTCGCGGTGTGACCCGGCCAAACGGGGAAAACC
Clone Rv349
:::::::::::::::Rv349SP6.seq:::::::::::::::
CAATACTCAAGCTTGACTGGGCCCGCACTTCGGCGCCACCCACACCGTCAACGCCCGCGAAGTCNACGTCGTCCAGGCCATCGGCGGCCTCACGGATGGATTCGGG
CGCGGACGTGGTGATCGACGCCGTCGGCCGACCGGAAACCTACCAGCAGGCCTTCTACGCCCGCGATCTCGCCGGAACCGTTGTGCTGGTGGGTGTTCCNACGCCC
GACATGCGCCTGGACATGCCGCTGGTCNACTTCTTCTCTCACGG
:::::::::::::::Rv349T7.seq:::::::::::::::
TCGACGGTTTGGCGGCCTTAAATGCACTGAGGTCGTCATTGACCCCACAGCGGAAATGCCGACTATTCGCAGGCCTCCTTCGGCTTGGCTGCCGGAGAGGGCGCTC
CGCGGGAACCGCATGCAGGTATATGACCTCGGTTTCTCGGGTGCTACCGCGTGCCTTGNTANGATNANCTCGGCGTTGGAATTGTCCAGCCGGCCCCAATTGATCG
AGCGCANATTCGTACACNTGGCCGGCGGCGACATACGCTTCACCGTGGATCTGTCTCCACACGGACCGCCCTGCGGGATCCTGCTCACGGGTAANGGAACTTACGT
GGCACTCGG
Clone Rv34
:::::::::::::::Rv34SP6.seq:::::::::::::::
GACCACGCCAGGCTAATCACGTGACGCTACCGAATACCTNCCTAGTGGTGCAGGCTCCCGCTGGAAATGGCCCTGTACCAACTCGCGCACCGGTGCCAG
:::::::::::::::Rv34T7.seq:::::::::::::::
CGGCACCCGACCCCTTTGAGCCGTCCGCCGTGGCCGCGGTGGAACTGCCGACGAGGGACTGATCGTGCTGGGCAAATTGGTCGATGGCACGCTGGCCCCCCGATCT
GAAGGTCN
Clone Rv350
:::::::::::::::Rv350SP6.seq:::::::::::::::
CTCAAGCTTGCCGTTACCCCGACTTCCGGAGGGACACCATGAGCACCGCCAGCCGAGCACGAGGCCAAACTCCGCCGACGCAGGCCGGTTGGACTTGTCGTGCTGG
ACAAGGGGTTTAGCCGCCGAAGCAGTGACGTACATGGGCGAAAAGCAGTTCGCCTGTCGACCGACGGNGCNNACCGTGAGGCTAGGGAAGCGAGGAGCACATGGCC
GCCGACCCGCAATGTACACGCTGCAAGCAAACCATCGAACCCCGGATGGCTATNCNTCACCGCCCATCGCCGCGGT
:::::::::::::::Rv350T7.seq:::::::::::::::
CATGTCGCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGGATCCCAAGTGCGGATGATCGGGCCCGCCTACGTCGTGGTGTACCTCGTCGGTAACA
ACGAAACCGAAGCGTATGACTCGGTCCACGCGGTGCGGCACATGGTGGAACACCACACCGCCACCGCACGGGGTGAAGGCCTATGTCACCGGTCCGCAGCACTCAA
TGCCGACCAGGCCGAGGCCCGGAGACAAAGTATCGCTAAGGTCACCGCGATCACGAGCATGGTGATCGCAGCAATG
Clone Rv351
:::::::::::::::Rv351SP6.seq:::::::::::::::
ATACTCAAGCTTCGGTACGGTGGCGGGCCGTGCTGCTGGCCGCGGTCGCGGCGTGCGCGGCCTGCGGTCTCGTTACNAGCTCGCGCTGCTGACACTGGCGCGCNAG
CCTGAACGGCGGCGGGATCGTGGCCACCTCCCTGATCGTCGCGGGCTACATAGCCGCGCTGGAGCAGGCGCCTTGCTGATCAAGCCGCTACTTGCACACGCGGCCC
ATCGCGTTCATCGCCGTGGAGGCGGTGCTGGGCATCATCGGCG
:::::::::::::::Rv351T7.seq:::::::::::::::
TGTCAAGTCCTTTCAGATCTCNTTTTTATGACATGACTGGAGATCTGTCTAGATTGCAGCTCCTGTGAGCGTGGGTACCGGATTCAAGCCGGTCGGTCACGCCCCG
GTGGTACCGGCTTTGCGGCAGTGCTCGGCCTCGAGTTCGGCGATCGCGCGCGAAGTGCGTTCGCGCAGCAAGATCGCGGCCGTAATGCCGGCGATGACCGCGATGA
CCAGCGCGATCCAGGAGAACCGTTCCAACCAGTGCTGGGCGGCCATCCCGGCGAAGTAGACCAGTGCAGTGGTGCC
Clone Rv352
:::::::::::::::Rv352SP6.seq:::::::::::::::
CAATACTCAAGCTTCAAAACAGGCCTGTTGTGGGCGCACCCGGCTCGCCGAGTTCTGCACGCACCGCCTCANTGCGGCCCGCACCGCCGGCATCTCCCCGGTCACG
CAGGGCCGCGGCCCGCGCCGCANCGACGGNGTGTTCGCGCAGTTCGCCGTCAATGATGCTGACCTGATCGGCCACCCGGGCGTTCTCGGGCGTCGTCCGTTCACTA
ATCGCGGTGCTC
:::::::::::::::Rv352T7.seq:::::::::::::::
TACGCTGGCGCTGGAGGGAGCCANNTACAACATCCACGCCAATGCTCTTGCCCCGATCGCGGCGACCAGGATGACCCAGGACATCCTGCGCCCCGAAGTACTGGAA
AAGCTCACACCCGAGTTCGTCGCACCGGTGGTGGCCTACCTGTGCACCCGAGGAGTGTGCGACAACGCATCGGTGTACGCGTCGGTGGTGGCACAGGTGCAGCGAG
TTGCGCTGTTTGGCAACGACGGCGCCAACTTCGACAAACCGCCGTCGGTACAAGATGTTGCGGCGCGGTGGGCCCGAGATCACGATCTGTCCGGTGCGAAAATTGC
TG
Clone Rv353
:::::::::::::::Rv353SP6.seq:::::::::::::::
GCTTTTCCGTCCGTCNNCGCTCAACCGCGTGAGGCCGAAGCGGNTGGTTACCGACTCCCTGTTTGTGATGGACCACTTCTACCAACTGCCCATGTTGGGGACNCCC
GACCAGCCGATGCTGGAGGCCTACACGGCCCTTGGTGCGCTGGCCACGGCGACCGANCGGCTGCNNTGGGCGCGTTGGTGACCGGCAATACCTACCGCAGCCCCGA
CCCTGCTGGCAANATCATCACCACGCTCGACGTGGTTAGCGCCGGTCGAGCGATCCTCGGCATTGGAGCCGGTTGGTTGANCTGGAACA
:::::::::::::::Rv353T7.seq:::::::::::::::
CNGCTTTTTAATGGCCTTGACNTGGGCGNGCCGGCCACCGGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCGCCGACT
TTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGC
GGTTCCGCTGACCAATACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAGAGCCACTGCGATCGGTGCCGATCGTGGGG
AACCCACTGGCGAACCTGGTTCAACCAAACTTGAAGGTGATTGTTAACCTGGGCTACGGCGACCCGGCCTATG
Clone Rv354
:::::::::::::::Rv354SP6.seq:::::::::::::::
CTCAAGCTTGCCGGGAGGGTGCATGGCCGACTCGGATTTACCCACCANGGGGCGCCAACGCGGTGTCCGCGCCGTCNAGCTGAACGTTGCTGCCCGCCTGGAGAAC
CTGGCGCTGCTGCGCACCCTGGTCGGCGCCATCGGCACCTTCGAGGACCTGGATTTCGACGCCGTGGCCGACCTGAGGTTGGCGGTGGACGAAGGTGCACCGCGGT
TGATTCGCTCGGCCTTGCCGGATGCCACCCTGCGCCTGGTGGTCGATCCGCGAAAANACGAANTTGTGGTGGAGGCTTCTGCTGCCTGCGACACCCACNACGTGGT
GGCACCGGGCAGCTTTAGCTGGCAT
:::::::::::::::Rv354T7.seq:::::::::::::::
CCGACGCCGTCGTGGCCACCAACACCGCGACCAGCACCGTGACCCGGACCGGGGTGCCGCGCGAACCGGTCTTGGCCAATTGCCGCGGCACCAAGCCGTCGGCGCC
CATGGCGAACAGCACGCGGCATTGCCCGAGCATCAACACCATCACCACCGTGGTAAGCCCGGCCAGCGCCGACGGAGATGATGCCGCTGGGCCCAGTACACCCCCG
TTGGCCTGGAACGCGGTGGCCAGTTTGCCGGCCGCGGCCCGGTACGGTCCGCAGTTGGGTGTATGGAACCATGCCCGACAGCACCACCG
Clone Rv355
:::::::::::::::Rv355SP6.seq:::::::::::::::
TTNACTGGCCTTTGGTCCACACTAGACAATACTCAAGCTTCCAGGACATCGTCATCGCGACCAAAACCGCGAGCTAGGTCGGCATCCGGGAAGCATCGCACCGGG
TGGCGCCGAGCGCCGCTGCCGGCAGGCCGATTAGGCGGGCAATTAGCCGCCGCGGCTCCCGGCTCCGANTACGGCGCCCCCGAATGGCGTCACCCGCTGGTAACC
ACGCTTGCGCGCCTGGCGGCGGCCTGCCGGATCAGGTGGTAAATGCCGACA
:::::::::::::::Rv355T7.seq:::::::::::::::
NGACGTCTTCCATCCGCGCGTCGTTTTGGCGGGTTGGCCACAGCAGCCCGCCGGTGACGGCGACGATGCTGGGCTGGTTGCGGCCCTGCGGCACCGCGGCTTGCAT
GCTGTTGGCTGTCTTGGGACGATCCCCCGAAATAGTCCACGCGGATCTGGTGATTTTGCGGGCTACCGCGATTACCCCGCGCGGCTCGACGAGTTTTGGCCCTGGA
CTACCCGCGTGGCCAATCTGCTGAACTCGCGGCCGGTGGTGGCCTGGAATGCGAGCGCCGTTACCTA
Clone Rv356
:::::::::::::::Rv356SP6.seq:::::::::::::::
CTTCCTCCTGAGTACCNCCCGTNTACTTTGGGATGGGTAAAAAGGCGAATCNCCGTTTGGTCACGAACGCCGGGAGGGACAATCTCGGGCGCCCGTGGGTCTCCGC
GGGAANGCCCGAATGTACGGTGTCTCGACACTTCCCNTCCCCTCCG
:::::::::::::::Rv356T7.seq:::::::::::::::
GAGCATCGGGACNTACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACGACGCCNGCGACCACATTCAGCAGATGGCCAGCGCG
TGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCNTGATCNACATCGTCACCGCCGCACCACTGCCCGCCGCCTCGGGTCACGAGCCGTTGC
CGCCCNCAGCGGACGATCACNTCGCCGCGATCGCC
Clone Rv357
:::::::::::::::Rv357SP6.seq:::::::::::::::
TACTCATGANCATCCTTTAATCANNGCTTTGCGTTTTTTTATTAAATCTTGCAATTTACTGCAAAGCAACAACAAAATCGCAAAGTCATCAAAAAACCGCAAAGTT
GTTTAAAATAAGAGCANCACTACAAAAGGAGATAAGAAGAGCACATACCTCAGCACTTATTATCACTAGCGCTCGCCGCAGCCGTGTAACCGAGCATAGGGGAGCG
AACTGGCGAGGAAGCAAAGAAGAACTGTTCTGTCAGATAGCTCTTACGCNCA
Clone Rv358
:::::::::::::::Rv358SP6.seq:::::::::::::::
CTCAAGCTTCAGGTCAATGTGCNCCAAGCCCTGACGCTGGCCGACCAGGCCACCGCCGCCGGANACNCTGCCAAGGCCACCGAATACAACAACGGCCGCCGAGGGT
TCGCANCCCAGCTGGTGACCGCCGAGCANANCGTCAAAAACCTCAAGACGCTGCATGACCAGGCGCTTANCNCCGCANCTCAGGGCCAAGAAGGCGTCAAACGAAA
TGCGATGGTGCTGCACCANAAGATCGCCGAGCGAACCAAGCTGCTCAGCCNG
:::::::::::::::Rv358T7.seq:::::::::::::::
CATGGTGGCACTGTAGCGACGTGCTGCAATCAAGGTCATGCCCGACTCTGGTCAGCTCGGANCCGCTGACACCCCGCTAAGGCTGCTCAGCTCGGTGCATTACCTC
ACCGACGGCGAACTCCCCCAGCTTTACGACTATCCGGATGACGGCAGCTGGTTGCGGGCGAACTTCATCATCAGCTTGGACGGCGGCGCTACCGTCGATGGCACCA
GCGGGGCGATGGCCGGGCCCGGCGACCGATTCGTCTTCAACCTGTTGCGTGAACTTGCCGACGTCATCGTGGTCGGCGTGGGCACCGTGGCATTGAGGCGCTACTC
CGGCGTCCGGATGGGTGTCGTCCAGCGCCAGCAC
Clone Rv359
:::::::::::::::Rv359SP6.seq:::::::::::::::
TACTCAAGCTTGCGGGTGATCGCCTTGGTCAACGGCACCGTGATCGGATCGGGGTCNACCGCACAAATGGACTGGAGCTTCGGCGCAANCATCGCCTATGCCTCGC
GGGGGGTGACGCTGACCCCGGTGACNTGTTCGGCTCGGGCACGGTGCCCACCTGCACGCTCGTCTATCACCTCGGCCACCGGAATCATTCCCGGGCTGG
:::::::::::::::Rv359T7.seq:::::::::::::::
GTTGGNGCCTCGTCGGCGAACAGTTCTCGCACGATTTCCGGATTAGCGGGACTGGTCACCAGTTGGGTATGCGGGAAGGCGCTGACGTTCGGCCGGATTAGCTGTT
TGATGGACGCGGTGGTGATGTTCTGATCACGGAACTGGCTGTAATAGCCCAGGGTCGCCACGTTTCATCCGGCCCCGGACCCCGGCGCACCGAGCGTGTCGCGCAG
GTATGCGACGTGATTTTCGCTGAAGTCCCCGTACCCGGAGAACT
Clone Rv35
:::::::::::::::Rv35SP6.seq:::::::::::::::
TGCTTCCGGCTCGTATGTTGTGTGGAATTGTGANCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAAT
ACTCAAGCTCCAGGTCAATGTGCGCCAAGCCCTGACGCTGGCCGACCAGGCCACCGCCGCCGGAGACGCTGCCTTTGTCACCGAATACAACAACGCCCGCCGAGCG
TTCGCAGCCCAGCTGGTGACCGCCGAGCAGAGCGTCGAAGACCTCAAGACGCTGCATGACCAGGCGCTTAGCGCCGCAGCTCAGGCCAAGAATGCCGTCGAACGAA
ATGCGATGGTGCTGCGGCATAAGATCGCCGAGCGAACCAAGCTGCTCAGGCCAGCTCGAGCAGGCGAAGATGCACGAGCA
:::::::::::::::Rv35T7.seq:::::::::::::::
CAGGCATGCAAGCTTCGGAGGCAGACCCGTGCATGGTGGCACTGTAGCGACGTGCTGCAATCAAGGTCATGCCGACTCTGGTCAGCTCGGAGCCGCTGACACCCCC
GCTAAGGCTGCTCAGCTCGGTGCATTACCTCACCGACGGCGAACTCCCCCAGCTTTACGACTATCCGGATGACGGCACCTGGTTGCGGGCGAACTTCATCAGCAGC
TTGGACGGCGGCGCTACCGTCGATGGCACCAGCGGGGCGATGGCCGGGCCCGGCGACCGATTCGTCTTCAACCTGTTGCGTGAACTTGCCGACGTCATCGTGGTCG
GCGTGGGCACCGTGCGCATTGAAGGCTACTCCGGCGTCCGGATGGGTGCTGTCCATCGGCCA
Clone Rv360
:::::::::::::::Rv360SP6.seq:::::::::::::::
TACTCAAGCTTGGGGTGGCGCTGTCGGTCGGTGTGCTTGGCGGCGTCGGTATCAACACCGCCCACGAAATGGGGCACAAGAAGGATTCGCTGGAGCGGTGGCTGTC
CAAAATCACCCTCGCCCANACCTGCTACGGGCACTTCTACATCGAGCACAACCGTGGCCATCACGTCGGGGTGCCACACCGGAGGACCCCCGGCGTCGGGGGGTTC
GGCANAACGTTGTGGGANTTCCTGCCCCGCANTGTTATCGGCGGCTTGCGCT
:::::::::::::::Rv360T7.seq:::::::::::::::
GGCCATCGCCACCGCNCCGCGGCGAACGCTCAAAGGCACCTACTGGCACCAAGGCCCCACACGTCACCCTGTGACCTCCTGGCCGACCCCCGCCCGAGGTCCTGGC
CGTTACCACCGAACCGGGCGAGCCGGGAGTCTGGTACGCATCGAACAAAGAGCAAGGTGCATGGGCGGAGTTGTTCCGCCACTTCGTCGATGACGGGTCGATCCAT
TCGAGGTCCGTCGCCGCGTCGGTCGAGTGGCGGTCACACTCCANGTACTCGACCTCACAGACGAGAGGACTCGATCCCATCTAGCGTGTGGACGAAACAGTCTTCT
GTCCGACGACTACACCACCACCCAGGCCATCGC
Clone Rv361
:::::::::::::::Rv361SP6.seq:::::::::::::::
GCTTGCGGGTGATCGCCTTGGTCACGGCACCGTGATCGGATCGGGGTCNACCGCNCAGATGGACTGGANCTTCGGCGAANTCNTCGGCCTATGCCTCGCGGGGGGT
GACCCTGACCCGGGTGACNTGTTCGGCTCGGGCACGGTGCCCACCTGCACGCTCGTCAAGCACCTCNGGCCACCGGAATCATTCCCCGGGCTGGCTGCACNACGGC
GACNTGGTCNCCCTCCAGGTCGAAGGGCTGGGCNAAACAANGCAGACCGTCCGGACANCGGCACTCCTTTTTCCGTTGGCTCTTCGGCCGAATCCGGACGCCNAAC
CCGACCGGCG
:::::::::::::::Rv361T7.seq:::::::::::::::
GTTCTCGCACGATTTCCGGATTAGCGGGACTGGTCACCAGTTGGGTATGCGGGAAGGCGCTGACGTTCGCCGCGATTAGCTGTTTGATGGACGCGGTGGTGATGTN
CTGATCACGGAACTGGCTGTAATANCCCAGGGTCGCCNCGCTTTCATCCGGGCCCGGACCCGGCGCACCGAAGCGTGTCGCGCAGGTATGCGACGTGATTTCGCTG
AAGTCCCCGTACCCGGAGAACTCGAACACGCTGAGGCGCTCGTCACCGTCGTNNCGGCGACCAAGCGCGGCGAGCAACTGCGCAAAATCGTTAAGANAGGTCGAAT
CGTTGAAATTCGGCACCACCTGCACC
Clone Rv363
:::::::::::::::Rv363SP6.seq:::::::::::::::
CACAAGACAATACTCAAGCTTCAGGTCAATGTGCNCCAAGCCCTGACGCTGGCCGACCAGGCCACCGCCGCCGGANACGCTGCCAAGGCCACCGAATACAACAACG
CCGCCGAGGCGTTCGCAGCCCAGCTGGTGACCGCCGAGCANANCGTCNAAAACCTCAAGACGCTGCATGACCAGGCGCTTANCGCCNCAGCTCAGGCCAAGAAGGC
CGTCGAACGAAATGCGATGGTGCTGCAGCANAANATCGCCGANCGAACCAAGCTGCTCAGCCAGCTCGAGCAG
:::::::::::::::Rv363T7.seq:::::::::::::::
CCACCGTGCATGGTGGCACTGTAGCGACGTGCTGCAATCAAGGTCATGCCCGACTCTGGTCAGCTCGGAGCCGCTGACACCCCCGCTAAGGCTGCTCAGCTCGGTG
CATTACCTCACCGACGGCGAACTCCCCCCAGCTTTACGACTATCCGGATGACGGCACCTGGTTGCGGGCGAACTTCATCAGCAGCTTGGACGGCGGCCTACCGTCG
ATGGCACCAGCGGGGCGATGGCCGGCCCGGCGACCGATTCGTCCTTCAACCTGTTGCGTGAACGGTGCC
Clone Rv364
:::::::::::::::Rv364T7.seq:::::::::::::::
GCTTTCCGCCGATACCNCCATGTCCCGCACATCCAGGACTTCTGGGGGGATCCGCTGACAGCGGCGGGCATCCCAAAGTGCGGATGATCGGGCCCGCCTAGTCGTG
GTGTACCTCGNCGGTAACAACGAAACCGAANCGTATGACTCNGTCCACGCGGTG
:::::::::::::::Rv364T7.seq:::::::::::::::
CAACCCGANTTGGCTTTCGGCGCCNTCGGTGAGGACGGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACACCTCGATGCTGCCGCCATGGACGCGGTCG
AACGCAAGCAGCTGATCGATCTACNACGCCGNGNGGAACGCTTCNGCCGCGGGCGTGACCGCNTCCCGTT
Clone Rv365
:::::::::::::::Rv365SP6.seq:::::::::::::::
GGGATGGGCAAAAAGGCGAAGCACCGCGTGGCCACGAACGCCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGGAAGGCCGAACGTACGGCGTTCAAC
ACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGGTAGCACCCTGGCCGGGCGATGATCTGCCAGCGTCCCCGCGGGTAGTCGCCGCCCGG
GCGG
:::::::::::::::Rv365T7.seq:::::::::::::::
CAGCAGACCAACAAGAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACGACGCCAGCGACCACATTCAGC
AGATGGCCAGCGCGTGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCACGGTT
Clone Rv366
:::::::::::::::Rv366SP6.seq:::::::::::::::
CTCAAGCTTGACTGGCCACCCACCGGCATGACCACCGACAGGCCCGACTGGTCGTACCACTCGAACGCCGGGGTGTTTGA
:::::::::::::::Rv366T7.seq:::::::::::::::
TTTGGTGCCCGGAATGGCGAGTCCCATTTANTCGCTGATTTGTTTGAACAGCGACGAAACCGGTGTTGAAAATGTCGCCTGGGTCGGGATTCCCTCTCCAAGCAAG
AGTAACTGGCCCCCAAATAAGTTACTCGTCGTCTTGCAAAGACCGCTACCCCGATGCCATTATGTGTTCCTTACGCTCNNNNTTCCGGTGCGCCCATCATTATCTG
CACCTTTGCACTGCACATTGAGCTTAGCAGCGCTCG
Clone Rv367
:::::::::::::::Rv367T7.seq:::::::::::::::
GAATTNGCTTTCGGCGCCATCGGCCCAGGACCGCGTGCGGGTGCTCAACGACGACGTCGTCCGCGGGACACACCTCGATGCTGCCGCCATGGACGCGGTCGAACGC
AAGCAGCTGATCGAGCTACAACGCCGCGCGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCNGTGATCGTCGATGACGGCATCGCCA
CCGGAGCGACGGCCAAGGCGGCGTGCCAGGTCGCCGGGCCCACGGTGCGGACAAGGTGGTGCTGGCGGTCCCGATCGGCCAGCGACATCGTGCCGCCGAGATTCCG
CGGGTACGCCGATGAAGTGGTGT
Clone Rv368
:::::::::::::::Rv368SP6.seq:::::::::::::::
TAAAGCTTTCGTCAGTTCATNGNGCCCCCGGACCAACAAAAGCATCGGGACATACGGAGTCAACTACCCGGCCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCN
AACGACGCCAGCGACCACATTCAGCAGATGGCCAGCGCGTCCGGGCCACACGAGGTTGGTGCTCGCGGCTACTCCCAGGGTGCGGCCGTGATCNACATCGTCACCG
CCGCACCACTGCCCGGCCTCGGGTTCACGCAGCCGTTGCCGCCCGCAGCGGACGATCACNTCGCCGCGATCGCCTGTTCGGGAATCCCTCGGGCCGCGCTGCCGGG
GCTGATGAGCGCCCTGACCCCTCAATTCGGGTCCAANACCATCNACCTCTGCAACAACGGCGACCCCGATTGTTCGGACGGCAACCGGTGGCGANCGCACCT
:::::::::::::::Rv368T7.seq:::::::::::::::
CCGGGAGGGACCATCNCGGGCGGCTNCGGCTTCTCTCCGGAAGGTTCTANNGTNNGCGTTCNACNCTTCCCGTCGCCCTGCGACCGCCCGAACATTCGGGGGTATG
CNNGCANCCTGTNAGCATCCNGGCCGGGGC
Clone Rv369
:::::::::::::::Rv369SP6.seq:::::::::::::::
CTCAAGCTTCCGCATCAGATCGCTATAGAACCGGTGCGCGTCCCACCGAGTGGCTGGTCGCCTTCCAGCACGATCGTTACCGCGTTATCGGAATCAAACTCNCCCG
AACACCTGACCAACGCGCTTGATCGCCTGAATCGATGCGGCGTCGCTGGGGCTCATCGATACCGAGTGTGCTTTTCCGACCACTTCCAGTTGCGGTACGGCGAGAT
TGACAAAGGCGGTGAAGCCCAGCCAGAGCAGGACGATCACCNCCGCAAACCCGGCGGATTTGCCCG
:::::::::::::::Rv369T7.seq:::::::::::::::
GCTTGGCAGCCTGCGGCTGGGCGCCCTNGAGCTCTTCGATCTGGATCTCCGGACTCGAGATGCTCACTTGCCCGGCCGTGGACGTACCCATTGCGGCCGGGACCCC
AGCGCCCCCCAGGTGACCAGCGAGTTGGGCTGCACGCTGACCGGCCCGTCGGGTCGACGCCGGTAACGGTCAGCAGCTCCGANGTCCNCTGATCCCGACCGCAGCT
GCCAATGCGCGCTGGCAGCCGACGTGGATGTGCCGGGGCCTAGATCGCGGGGCAGCAGCGAGACCCGCGTCACCGACGGTCATCACCTTGCCGAGTTTAGGCCTGC
CGCAN
Clone Rv36
:::::::::::::::Rv36SP6.seq:::::::::::::::
GCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTNCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATCTAGGTGACACTATAGAATA
CTCAAGCTTGAGCCTATCGGGCTATCAGCTGGTTGATGTCCCG
:::::::::::::::Rv36T7.seq:::::::::::::::
CAGGCATGCAAGCTTGTCGTCTATCACATCCGACCACCAACGCCCGACGGCTCGGCAGAACGCCTCCGCATATGGGTCGACGACCAGCGGGTCGGACTTCTGGGGC
TGCCAGCGCTCGCGCCGTCGCGACAAACAGCGCGGTCGAACCGACACTCCTTGTGATGTCCCACCTATCACCTCGGTACGCACCCAATCGACGCTAGCGGGCCTAG
CTCAGCCCCGATCTTCCAGAGCTCCGCCCCG
Clone Rv370
:::::::::::::::Rv370SP6.seq:::::::::::::::
GCTTTTTGAGCGTCGCGCGGGGCGGCTTCCCCGGCAATTCTACTAGCGAGAAGCTCGGCCCGATACGGATCTGACCGAAGTCGCTGCGGTGCAGCCCACCCGCATT
GGCGATGGCGCCGACNATGGCGCCTGGACCGATCTTGTGCCGCTTGCCGACGGCGACGCGGTAGGTGGCAATTCCGGTCTACGCTTGGGCCTTTGCGGGACGGTCC
CGACGCTGGTCGCGGTTG
:::::::::::::::Rv370T7.seq:::::::::::::::
CGANCCTGTTCGACGGCTACCTGAATCACCCCGATNCCACCGCCGCGGCGTTCGACGCCGACAGCTGGTACCGCACCGGCGACGTCGGGGTGGTCGACGGCAGTGG
GATGCACCGCATCGTGGGACGCGAGTCGGTCGACTTGATCAATCGGGTGGATACCGGGTCGGCGCCGGTGAAATTGAAACGGTGCTGCTCGGGCCCATCCGACGTG
GCGGAGGCGGCAGTCGTCGGGGT
Clone Rv371
:::::::::::::::Rv371SP6.seq:::::::::::::::
NAAGCTTTGTCACACCCAAGTGTTTCNACCAGNCGCTCCATCCGGCGAAGTGGATACTCCCAGCAGGTAGCAGGTCGCCACCACGCTGGTCAGTGCCGTTCAGCTC
GCTTGCGGCGCTGCAGCAGCCAGTCCGGGAAATAGCTGCCCTGGCG
:::::::::::::::Rv371T7.seq:::::::::::::::
CCGCTGGNCGGCGGCGCTGGGCTGCGGTAACAATTACCACAACACTTTTCGGTAGCCGAACAGCGGCGCGTACCAGCGAAATGGCACAGCCACCGCAGTCGCCGAC
ATCCCGCGAAGATGGCAGTTTTCGTGCGGTCGAGCGGCGAAGGCCTAGCGTCATTGTTGCCTGGCAAGGTTGCTGGCCCCGG
Clone Rv373
:::::::::::::::Rv373SP6.seq:::::::::::::::
CTCAAGCTTCTTCTGCCCCTTGCCGTTNCGGATNACATCCCGCAGCGACTCGGCTTCGGCGTCGATGTCGAAGTTCTCGATCAGCTTCTGGATCGACTCCGGCGGG
ATGGCACCGGTGAAGCTACTCGCCGTAGCGGTCGACNAGTTCGCGGTAGAAGTTTTCGTCNACNATCAGCTGCTTGGGCGGGANCTTGGTGAAAGTGCTCCAATGT
CCTCCAACCGGTCCAGCTCACGCTGCGCGGTCACGGATCTGGCGCATCTCGCGCTCGCCGCCGTCGCGAACTTGCGCCGCCATCGGGCTTGGGGCC
:::::::::::::::Rv373T7.seq:::::::::::::::
GTTCACACCTACCTACTATGCCNCAATTCNCCGACACGGGTGGCATCAACACGGGCGATATAAGGTGGAAATCGCTGGGTGAACGTCGGGCTGGTGCGCTCGCTGC
AATCCGCGGCAACCGCGTGTTGATCGGATTCTCGTTGCCCGGCAAGACAATCGGGATGCAAAGCCGGGCAGCAATTCNCNCCNACACCATTCTTGGCCGTAAGAAC
CTGGAGATCGAACCCCGCGGTTCGGAGCCGTTGAAACCCAACGGTTTCCTGCCGTTGGCGCANACCACTACGCCATACCAAATC
Clone Rv374
:::::::::::::::Rv374SP6.seq:::::::::::::::
CTCAAGCTTTACGCCGACGCCGGCCTACACAACACCAAGGAAACGATTGCCTACTGCCGAATCGGGAACGGTCCTCGCACACCTGGTTCGTGTTGCGGGAAATTAC
TCGGACACCAAAACGTCAAGAACTACGACGGCAGTTGGACAGAATACGGCTCCCTGGTGGGCGCCCCGATCGAGTTGGGAAGCTGATATGTGCTCTGGACCC
:::::::::::::::Rv374T.seq:::::::::::::::
TCCCNCATGGGATAACGGGTTTAGATTTCNACAACGGCACCGTGTTTCTCAACAAGCCGGTCATCAGCTGGGCCGGCGACAACGGTATCTACTTCACCCGCTTCCG
CCCGTACAAGAAAAACCACTAGGCCACCATCGAGTCCAAGAACAACCACCTGGTCCGCAAGTACGCGTTCTACTACCGCTATGACACCGCCGAGGAACGCGGCGTG
CTCAACCGGATGTGGAAGCTGGTCAACGACCGCCTCAACTACCTCACCCCGACCATCAAACCGATC
Clone Rv375
:::::::::::::::Rv375SP6.seq:::::::::::::::
CTCAAGCTTGGGTGTTGCCGATCACCGGAAGCCNCATGATCAGCCAGTTTCGCGCCGCCCGGCATACGGCGGCGTACCGATCTCCGCGTCATACACCCCGGCGGTA
ATCGCCGACGGTGCCGGTTCGCGAGCCGAAGGTGACAACGCTGATTGAATCNAGTTCCANGTCCAGCGGGT
:::::::::::::::Rv375T7.seq:::::::::::::::
TNAACAGCTCGCGGCAGCCCACGACCTGCTGCGTCGGATTGCCGGCGGCGAGATCAATTCCAGGCAGCTCCCGGACAATGCGGCTCTGCTGGCCCCGCAACGANGA
CTCGAGGTCACCCCGGTGCCCGGGGTCGTGGTGCACCTGCGATCGCACAGGTTGGCCCACAACCGGCCGCTTGATGNNNNGTCGGCAAGCCCCGGCAGTTNGCCAA
CCCAGCGTGATCANGCTCGGCTCGCGAGTTCGGCGAAANAAGTGGCTCGCCTGATCACCTACCATCGGCCANGATCTGCTGTCA
Clone Rv376
:::::::::::::::Rv376SP6.seq:::::::::::::::
GCCANACCGGCTTGGCGTCGACTCCCGTTCNGCACATCATACGGTCCCCGGTACTGTCCAACTGCGCCGGTGCGCTAGCCAAACGTCACGACTCTCAGTGTCCCAG
TTCGTGATCCGGCCGGTGGCGCCGCTGCGGCGGGGCTNATNTACTTCGGACTNATTATCTCATCCAAAGGACACGGGGCCGGTGGCTGGAATCCCATGGTGCCGAT
CGGCCACACAN
:::::::::::::::Rv376T7.seq:::::::::::::::
CCGACCTGGTATCTTCCGATAGCCGCGTTGATATCCGGTCTGATCTCCTGCCCTTAACGCCCGATCTCAGCAGGTCCCCATGCAAAGATCCGAAGGTGTCCCNGAT
CTAGGGGTCCTCGTCCTCCAGATGATGGAGCAAGTCGGCCC
Clone Rv377
:::::::::::::::Rv377SP6.seq:::::::::::::::
CTCAAGCTTCGGCTCAGGCGGCGCTGCCGGTAACGTCGCTGACCGGTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCAAGACACTGGCGCA
GGCTATCGCACCCGTTATCGGCTACAAACAAATCGCGGTATGC
:::::::::::::::Rv377T7.seq:::::::::::::::
CATCACCTGNTTCATGAACTGGAAGCACCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCAGCCTCTCGTCGGCGGTCGGGTGCAGGTGCTCGGGCAGCTCGGCC
GCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACGAACGACGCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAGCGCGTTCTCC
ACCGACCGGGCCCGGGTGTGGGGTGT
Clone Rv378
:::::::::::::::Rv378SP6.seq:::::::::::::::
AGCTTAGCTTCCCGCCCCGGCAATAGGGCTCCAGCTCATCCGGTGTGACCAGATAGGGGCCCAGGTGATACCGCTGTCTTTGCCCTTGGCCTGTCCGATGCGCCAG
CTGGCCCTCCAGCATCTGCAGGTCCCGTGCGGACCAGTCGTTGAAATGGTATAGCCGATGATCGACCG
:::::::::::::::Rv378T7.seq:::::::::::::::
CCNGAACAGAAGCGGNGGTTCCTACCGCGGTGTGCGGCCGGCGCGATATCGGGCCTTTTTACTACCGAACCCGATGTGGGCTCCGATCCGGCGCCCATGGCATCGA
CGGCGACGCCGATCGATGACCGCCAGGCTTACCACCTT
Clone Rv379
:::::::::::::::Rv379SP6.seq:::::::::::::::
CTCAAGCTTGCGCGACTCGACAAGCATTCTTGACAGTTGTTTTGGCTCGGCATGGTTAGCCAAGGTTCTGCGGTCCCACCAGATCATCTTGGTCCGGTAGCGCTCG
TCCGGGTATGCTGCGCCGGGATTCTCGCTGCTATTACTCCCCCCCGAAGAACGCCACCGGTCCAGCGC
:::::::::::::::Rv379T7.seq:::::::::::::::
GCNAGGCGGTATAGCTTCCCGTCGTACCGGCGACCGCCAGCCGAGAAGCTCGTTTTCCCAGTGTTGCTGGGGATTCTCACGCTGCTGCTGAGTGCGTGCCAGACCG
CTTCCGCTTCGGGTTACAACGAGCCGCGGGGCTACGATCGTGCGACGCTGAAGTTGGTGTTCTCCATGGACTTGGGGATGT
Clone Rv37
:::::::::::::::Rv37SP7.seq:::::::::::::::
GTGTGGAACCGTGAGCCGGATAACAATTTCACACAGGAAACAGCTNTGACCTTGATTACGCCAGCTATTTAGGTGAGGCTATATTAATACTCAAGATTGCGGTCGA
GCACATCGGCCCAAGAACCGCCGAAGGCACGGCGGAACGCCTGCGGCACATGGGGCGACGACCAGCGGGTCGGACTTCTGGGCTGTCCAGCCGGATCGCGCCGTCG
CGA
:::::::::::::::Rv37T7.seq:::::::::::::::
CACTGTCAGACATATGCGCCGCTCCTCCTCATCGCTGCGCTCGGCATCGTCGCCGGCGGTCATGGCGTCACCCTACCCAAGCCGAAACGCGAAACGAGAACGTGTT
CCATTATTAGGGTGTGAGCACCAATACCAGATGCTCACCAGGAACTCACGCAGCACCGGGACGGATGTCAGCCACCACGCCCATCTGGGGTGGTAGCGGGGGAAAT
ACGGCTAACGCGGCTCCGGTGCCGGCAGCCCAGCGCAGCCCTCGGCGGCGGACACGGCAAACAACGACGACCCATAGTTGTTCTTTGCCGGATGGCCCCGTGTTGC
GGACATATCGGGCGGCGGCGCGGGCGCCGCCGAGGTAGTGGCTGAGGCCCATCTCGTGCCCCGCCGAATGGCCCCAGCCAAACCGTGTA
Clone Rv381
:::::::::::::::Rv381SP6.seq:::::::::::::::
CTCAAGCTTTTACGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCGCAACATGAGCCANCCTCTCGTCGGCGGTCGGGT
GCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACCCTGAAACCAGCTTCCATATCCCGCGACNAACGAC
:::::::::::::::Rv381T7.seq:::::::::::::::
CTCAGAAGCCGCTAGCTGGTAGAGTCGCTGACCGGTGCACGTGGCGNCAATGTGCGCTGCCGGTTCGCG
Clone Rv382
:::::::::::::::Rv382SP6.seq:::::::::::::::
CTCAAGCTTGCGCTCATCAAGCGCGAACAGCAGGGCGGTCGGCTGGTCGCCATGACGGGTGACGGGACCAATGACGCACCCCGCGCTCGCGCAAGCCGATGTCGGG
TGGCNATNAATACCGGCACCCAGGCGGCCCGGGAAGCCGGCAACATGGTCNATCTCCACTCC
:::::::::::::::Rv382T7.seq:::::::::::::::
ACTTCTATTTCGACTGGTGTGCTGTGGCGCGATCCGACTGCCGGCGTGGTCAAGGCCGGCCAGTTGTGGGATNCCACAGGCAC
Clone Rv383
:::::::::::::::Rv383SP6.seq:::::::::::::::
GCTTGTCGTATTCCGTGGCACTGTCAGACATATGCGCCGCTCCTCCTCATCGCTGCGCTCGGCATCGTCGCCGGCGGTCATGGCGTCACCCTACCCAAGCCGAACG
CGAAACGAGAACGTGTTCCATTATTAGGGTGTGAGCACCAATACCGATTGCTCACCAGGAACTCAC
:::::::::::::::Rv383T7.seq:::::::::::::::
CGATATTCGTCGGCCGCGTTGTCTCGACTGGGTCGCGT
Clone Rv384
:::::::::::::::Rv384SP6.seq:::::::::::::::
GACCTCGGCCACCAAGCCGGACGCGACCGTCGAGGTGGCGATCCGGCTTGGCGTCGACCCGCGTAAGGCAGACCACATGGTCCGCGGCACGGCCANCCTGCCACAC
GGCACTGGTAAGACTGCCCGCGTCGCGGCN
:::::::::::::::RV384T7.seq:::::::::::::::
CCGGAAGTCTAGGGGACGACCTACTCAGCGCAAAATGTCGCTAATGTGAGTCCGCCCCACCAGGGCAGATCAACCCATGTCGATGATGACCTACCCGGATAGGGGA
TTGGCGGT
Clone Rv385
:::::::::::::::Rv385SP6.seq:::::::::::::::
AGCTTCAGTTCCTCCACGACGCGTTCCCAAATGATTTCCCGATCCCACAATCTCGGTTCAGATACAGGTCGCCATAGCCCTTACTTCGGNAACGCGTGGCCCGATT
GGCCCTGCCGCTG
:::::::::::::::Rv385T7.seq:::::::::::::::
CCGCCTACGGGTCGAACATGCATCCCGAGCCGATGCTCGAGCCGCGCACCCCACTCGCCGATGGCCGGAACCGGCTGGTTACCCGGGTGCCGGCTGACC
Clone Rv386
:::::::::::::::Rv386SP6.seq:::::::::::::::
GCGGCTGGTTACGACTCCCTGTTTGTGATGGACCACTTCTACCAACTGCCCATGTTGGGACGCCCCGACCAGCCGATGCTGGAGGCCCTACACGGCCTTGGTGCGC
TGGCCACGGCGACCGAGCGGCTGCAACTGGGCGCNTTGGTNACCGGCAATACCTACCGCAGCCCGACCTGCTGGCAAAGATCATCACCACGCTCGACGTGGGTTAG
CGCCGGTCGAGCGATCCTCGGCATTGGAGCCGGTTGGTTTGAGCTGGAACACCGCCAGCTCGGCTTCGAGTTCGGCACTTTCAGTGACCGGTTCAN
:::::::::::::::Rv386T7.seq:::::::::::::::
GCCTTTCCGCACAATCTGTACCCCAGGACCNTCTAAAAATCGAATACGACGGCGTCGCCGACTTTGCGGGGTACCCGCTCAACTTTGTGTCGACCCTCAAGGCCCA
TTGCCGGCACCTACTACGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCANTNNTGTCGGTCCCACGATGACCCAGTA
CTACATCATTCGCACGGAGAACCTGCCGCTGCTAGAGCCCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTCAACCAAACTTGAAGGTGATT
GTTAACCTGG
Clone Rv387
:::::::::::::::Rv387T7.seq:::::::::::::::
GCAGCCAACAAGATGCATCGGGATCATACGCCGTCAACTACCCGGCCAACGGTGATTTCTTGGCGGCCGCCCCAC
Clone Rv388
:::::::::::::::Rv388SP6.seq:::::::::::::::
CTCAAGCTTGCCAAAGAGACCTCGTCCACCAAGCNGGACGCGACCGTCNAGGTGGCGATCCGGCTTGGCGTCCACCCGCGTAAGGCANACCANATGGTCGGCGGCA
CGGTCAACCTGCCACACGGCACTGGTAANACTGCCCGCGTCGCGGTATTCGCGGTTGGTGAAAAGGCCGATGCTGCCGTTGCCGCGGGGGGGGATGTTGTCGGGAG
TGACAATCTGATCGANAGGATTCAGGCGGCTGGCTGGAATTCGATGCCGCGATCGCGACACCGGGATCAGATGGCCAAAGTCGGGTCNCATCGCTCGGGTGCTGGT
C
:::::::::::::::Rv388T7.seq:::::::::::::::
CCACGGCGTGGATCAAGGTACCGGCCGGATGTTGCGCAATGGCAGGTTGTTGCCCGGCTTGATGTCGGCGTTAGCGCCGGATTCCACCACATCCCCCTTGCGAAAG
TCCGTTGGGTGCAATGATGTAGCGCTTCTCCCCATCGAGATAGTGGAGCAAACGCAATCCGTGCGGTACGGTTCGGGTCNTACTCGATGTGCGCGACCTGGCGTTG
ACACCATCTTTGTCATTGCGGCGAAAGTCGATCATCCGGTAAGCGCGCTTATGACCGCCCGCCTTTGTGCCGGGTGGTAATCCGGCCATGCGCGTTGCGTC
Clone Rv389
:::::::::::::::Rv389SP6.seq:::::::::::::::
GGCGGCTGCGTCGGCGAGATGATCGCCCGGTGCCACCCCGATCCGTGCCTCGGTCAGCGCCAACGTGCTTCCGGTCCGGCGACCACCATGTCGCATGCGCCCCGAC
:::::::::::::::Rv389T7.seq:::::::::::::::
GCAATCGCCTTGGCGGTCGCCGGTTGTCACCGGTGATCATCNCGGNGCGGATGCTCATNCGGCGCATTTCGTCNAATCGTTCCGTATGCCCACCTTGACCCGATGT
CTTCATATGGACCACGCCGATGGCCCNCGCGCTNCTG
Clone Rv38
:::::::::::::::Rv38SP6.seq:::::::::::::::
CCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTCA
AGCTTCCACATCGGTATGCCAAAGCATTGCGCCGCTATCGATTTCGCGCTGGCATCGCCAAGGTGGACTTCTTGCTCAGCGACGAGATCCCGTGGTCGGATCCGCG
GCTGCGGCGGGCTGCGACCCTGCATCTCGGCGGCACCCGTGACCAGATGGCGCGCGCCGAGGCAGACGTCGCGGCGGGACGCCACGCCGACTGGCCGATGGTGCTG
GCCGCGTGTCCGCACGTCGCCGACCCCGGCCGCATCGACGAAACCGGCCGCCGTCCGTTCTGGACCTATGCCCACGTGCCGTCGGGGTCCACGCTCGACGCGACCG
AGACCGT
:::::::::::::::Rv387T7.seq:::::::::::::::
CGCGTCCACCGCAGCGTGAGATTGGTGGCGCCATTCGTCGTGGTGTAGCTGCTGTTGGCGGGCGTCGCCGTATTGTGCGGGCCAGCCTTGTGCGGGGGCCGTTCTA
CCCACGAGTCGGCACTTCCGCAACCGCCCAGCTCGACCCGCGATTACGGCGGCCGCAACGGCCGCCGGAAGGCGTCTCGCAAGCGCCTTATCCTTTCGCAGGTTCC
AGATCCTTCCGCTACGTGGTCGCTCATCGGCGGCCCGGCCGAATGAGTACAGGTGAGGGTAACCGCTACAAATGAAGTTGGTCAGTGCTGGCCCCAACTGTGTAAT
GGTTGCCCGGCTCGGGTCACCACGTACATTCTGGCAAGGCGGGCGAGATTCGGTTCCTCGCGTCCTTGGCCGGTGGCGGTTCCCGGTTGTCCGTGGGCGTGTCGTG
TACGTGGTGTAAGTGTCGTGAACTCCTCAGTTTGGGCT
Clone Rv390
:::::::::::::::Rv390SP6.seq:::::::::::::::
CTCAAGCTTGCGCTGGATCTGGCGGCTGAGCCTGTTCTTGGCAACATGCCGAGGGATCGCCTTTTCCACCACGCGGTCGGGGTGCGTTGCATTAGCTCACCCCGAT
GGTGCGCTTGTGCAGGCCGCCGGGATACCCCGAGTGCCGGTAAACCATCTTGTGCTGCAGTTTGTCGCCGCTGATGGCGACCTTGTCGGCGTTGATCACANATGAC
AAGTCACCGCCATCGACATTGGGGGCGAACGTCGGCTTGTGCTGCCGCGCAGCAGGTTGCCGGCCCGCGACGGCAGGGGGGGAANCACCACGTC
:::::::::::::::Rv390T7.seq:::::::::::::::
TTTGGGATGCGGCAAAAAGGCGAAGCNCCGCGTGGCCACAACGCCGGGAGGGACAATCTCGGGCGGCTAGGGCTTCTCGCGGGAAGGCCCCGAACGTACGGCGTTC
AACACGTCGCGTCGCCCTCCGACCGCGAACATTCGGGGATGGCAGCAACCTGGTAGCACCCTGGCCGGGCGATGATCTGCAGCGTCGCCCGCGGGTAGTCGCCCCC
GGCGGCTACAGTCTGAAACGCGATGACCATCGATGTGTGGATGCAGCATCCGACGCAACGGTTCCTACACGGCGATATGTTCGCCTCGCTGCGCCGGTGGACCGGC
TGGGTCTATCCCGGA
Clone Rv391
:::::::::::::::Rv391SP6.seq:::::::::::::::
CTCAAGCTTCGTCATAAGACCATGGTGCGCTTTCTTTCACCCGTCCANAGTCGGGGGCATCCGCACCGGCTCGCATCGCATCATCGCTCCCACGAGCGGGGCTCAT
CAGCTTGGGCCATTTCAATGTACTTGATACCCCGCGCTGCGGGTAGGCCACTGCNACAATTCAAACACGGTGTCACACGGTGAATANTGTCNANATGGGCTCTGAT
CAACCGTCNCAAACCCGGTTC
:::::::::::::::Rv391T7.seq:::::::::::::::
GAATTCTGCGTGCACCGCTATGGGTTGCAGCAGCGGCTGGCGCCGCACACCCCACTGGCCCGGGTGTTTCGCCCCGAACCCGGATCATGGTGAGCCGAAAAGGCGA
TTCGCCTGTTCGATGCTGGGATTCGCCACCGCGAGGCCATCGACCGATTACTCGCCACCGGGGTGCGAGAGGTGCCGCATGCCCCTCCCGTCGACGTCTCCGACGA
TCCATCCGGCTTCCGCCGTCGGGTGGCGGTAGCCGTCGATGAAATCGCTGCCGGCCGCTACCACAAGGTGATTCTGTCCCGTTGTGTCGAAGTGCCTTTCCCGATC
GACTTTCCGTTGACCTACCGGCTGGGGCGTCTGCACAACACCCCGGTGAGGTCGTTTTGTTGCAGTTGGGCGGAATCCGTGCTCTGGGTTACACCCCCCCAACTCG
TCNCGGCGGTGCGCGC
Clone Rv392
:::::::::::::::Rv392SP6.seq:::::::::::::::
GCAGTTGGGAATCGCTCTGCAGCAAACCANTATTCTGCGCGACGTTCGAGAGGACTNTTTGAATGGACGGATCTACCTGCGCGCCGACGAGCTGGACCGATTAGGC
GTACNCCTCCGCCTGGACGACTCCGGGGCACTCGATGACCCCGACGGACGGCTCGCCGGCACTGCTGCGGTTCANTGCCNACCGCGCCCGANACTGGTATTCCTGG
GACTGCGGCTGATTCCACACCTCGACCGCCGCAGCGCTGCCTGCTGTGCGGCCATGTCTGGCATCTACCGCCGTCAGCTCGCCTGATCAGACCATCGCCCCCCCCT
CGTCTACCATCGGCGAATCTCTCTGTTCGGGACTGAANAANAGCCCAAGTGGCGGCGGCAGCACTGGNACTCTTCGGTAACCTGCNGACCGGATTGGACCGCTACC
G
:::::::::::::::Rv392T7.seq:::::::::::::::
TTGATCTGGACGTCTGAGCGGTGATCGGNCCGAACCTGAATTGTCCGGTAATGCCCAGCGCAGAAAGCANGGTGGTGGCCGGGGCGGTGAANCCGGCGTCGGCGCG
CACCGTCGAAGTCGATGTGGATTGCCGGAATGGGGATGTCCGGCACGGCGAAGCCGTAGTTCGCTTGTCCCGTGAGGCCCANGTGGATGGGGGGAAGGATCGTGGT
GTCCGGGATGATAATGGGGCCCGATGCCGCCGGTTGAAGTCCAGTGGATCGGGAATTCGGGAATCGTGATGCCGACGTTCAGGCCGAACAGGCCCTCCAAGTGCCT
CGCCACNAGATGCCGTTGCTGAAGTTGCCCGACATGAGGCGCCGGTGTCCACATTGGCCCGAATTGGCGACGCCGGTGTTGGC
Clone Rv393
:::::::::::::::Rv393SP6.seq:::::::::::::::
CACGTAGGCGCCGTCCATAAATNACTCCGCCGCGCTTCGCACNTCCTCGTANCGATCCTTGGCGAGCAGGTCANCCGGCGCTGCCCGTCNAGGACCCCCTTTTTTG
GCGTGCAGCCACTGGCCGACACCTCGGGGGTAAGCGAATCCGAGAGCAGGAGGACNAGGTCACGAANCTGCGCCAGCCCCGGTCGTACCGTCAGGGGGGTGTCCGC
CGGTCCGCCACCCCGCGTACCGCCCGATCGGACACCTGTATGACCGCGGCGACNTCGACCTGGGTGACGCCGAAGGGTTTCAGGGCATCNACNATCTCGGCTGGTC
GACCGCCCGGTCCAGGGTGACCGCCATCGTGGTTCCTCCGCAACTTCCGGTTCTACTACCGTAAACGCTACCG
:::::::::::::::Rv393T7.seq:::::::::::::::
CGGGGAACGGTCCTCGCACACCTGGTTCGTGTTGCGGGAATTACTCGGACANCAAAACGTCAAGAACTACGACGGCAGTNGGACAGAANACGGCTCCCTGGTGGGC
GCCCCGATCGAGTTGGAAGCTGATATGTGCTCTGGACCCAAGCAAGGACTGACATTGCCGGCCAGCGTCGACCTGGAAAAAAGAAACGGTGATCACCGGCCGCGTA
GTGGACGGTGACGGCCAGGCCGTGGGCGGCGCGTTTCGTGCGGCTGCTGGGACNCCTCCGACGAGTTCACCGCCGGGAAGGTCGTCGCGTCGGCCACGGGCGAATT
TCCGGTTCTTTCGCCGCGCCCCCGGATCCTGGGACCGCNGGCGCGCGCTTGTT
Clone Rv396
:::::::::::::::Rv396SP6.seq:::::::::::::::
CTCAAGCTTTGTCCGACAAGCGTTCCCGGGCGGTCAGCAAGCGAACGTCGGTTGGCCCACTGCGGGTCGATATTGCCGCCAGGGA
:::::::::::::::Rv396T7.seq:::::::::::::::
CGTCAGCACGGCGACGTCGCGNTACGCCGAGCAGTTACACAATCGCTCTGCAGCAAACCAATATTCTGCGCGCACGTTCGAGAGGACTTCTTGATTGGACTG
Clone Rv39
:::::::::::::::Rv39SP6.seq:::::::::::::::
CTGCATCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAA
TACTCAAGCTTCGCGCAGCGGCGGGTTGACCCGGTTCACGCCGTCATAGCTGGCCAATCTGGCCATCGTCGATCANCATGTGGTGGGGGGTGACCTCGCGGTGATC
GAAATACCCTGGTCCTTATCCCATTTCAGGATTTCGACGGTGCCCGCGGCCGACGCGTGACAGTGTGCACCCGGGCGCCGGCGTCACGGGCCAGCAAGGCGGTCGC
GGGACGATCGATTCCTCGGCGGCCCGGGGCCATCCCGGCCAGCCCCAGCCGCGCCGCCATGGGTCCCTCGTGCGCGCGACGGCGCCGACCGTCAGCCGCGCTCCTC
GGCGTGCTGGGCGATCAGCACGCCCAAACCGGTG
:::::::::::::::Rv39T7.seq:::::::::::::::
CCGACGCGCACTACGTGCTGGTGTCCACCCGCGACCCGCACCGGGCACGAGCTACGCAGCTACCGCATCGTCGATGGCGCTGTCACCGAGGAACCTGTCAATGTGT
CGAGCAGTACTGAACCGTTCCGAGAAAGGCCAGCATGAACGTCACCGTATCCATTCCGACCATCCTGCGGCCCCCACACCGGCGGCCAGAAGAGTGTCTGGCCAGC
GGCGATACCTTGGGTGCCGTCATCAGCGACCTGGAGGCCAGCTATTCGGGCATTTCCGAGCGCCTGATGGACCCGTCTTCCCCAGGTAAGTTGCACCGCTTCGTGA
ACATCTACGTCAACGACGAAGACGTGCGGTTCTCCGGCGGCTTGGCCACCGCGATCGCTGACGGTGACTCGGTCACCATCCTCCCCCGCCGTGCCGGTGGGTGAGC
GGACACATGACACGATACGACTCACTGTTGCATGCCTTG
Clone Rv3
:::::::::::::::Rv3SP6.seq:::::::::::::::
TGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGCCATCGATTACGCCAAGCTATTTAGGTGACACTATAGAAT
ACTCAAGCTTGCCGGGAGGGTGCATGGCCGACTCGGATTTACCCACCAAGGGGCGCCAACGCGGTGTCCGCGCCGTCGAGCTGAACGTTGCTGCCCGCCTGGAGAA
CCTGGCGCTGCTGCGCACCCTGGTCGGCGCCATCGGCACCTTCGAGGACCTGGATTTCGACGCCGTGGCCGACCTGAGGTTGGCGGTGGACGANGTGTGCACCCGG
TTGATTCGCTCGGCCTTGCCGGATGCCACCCTGCGCCTGGTGGTCGATCCGCGAAAAGACGAAGTTGTGGTGGAGGCTTCTGCTGCCTGCGACACCCACGACGTGG
TGGCACGGGCAGCTTTAGCTGGCATTCCT
:::::::::::::::Rv37T.seq:::::::::::::::
GGAAACACCGNCGCCGTCGTGGCCACCAACACCGCGACCAGCACCGTGACCCGGACCGGGGTGCCGCGCGAACCGGTCTTGGCCAATTGCCGCGGCACCAAGCCGT
CGCGCGCCATGGCGAACAGCACGCGGCATTGCCCGAGCATCAACACCATCACCACCGTGGTAAGCCCGGCCAGCGCGCCGACGGAGATGATGCCGCTGGCCCAGTA
CACCCCGTTGGCCTGGAACGCGGTGGCCAGATTTGCCGGCCCGCGGCCCGGTACGGTCCGCAGTTGGGTGTATGGAACCATGCCCGACAGCACCACCGATACCGCG
ACGTAGAGAAGGGTCACGACCCCAGCGACGCGAGAATCCCTCGAGGGACGTCTCGTTGAGGACGCTTGGTCTCCTCGGCCATGGTGGCCACGATGTCAAACCCCGA
TAAACGCGAAGAACACGATCGATGCCCGGCCAGCACGCCGTA
Clone Rv40
:::::::::::::::Rv40SP6.seq:::::::::::::::
CCTGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACNCTATAGA
ATACTCAAGCTTGTCCTCGGGCGTTGGCCTCGGCCAAGAAATCGTCGACGCCGGCCTCCTGTGCAATCGCCTTGGCGGTCGCCGGGTTGTCACGGTGATCATCACG
GTGCGGATGCTCATTCGGCGCATTTCGTCGAAGCGTTCCCGTATGCCCACCTTGACGATGTCCTTCAGATGGACGACGCCCGATGGCCCCGCGCTGCTGTTATCGG
TCCATTCCGCAACGACTAGGGGTGTCCCCCGCCGGAGCTGATGCCGTCGACAATGGCACCCAACCTCCTCAGTGGGGTGGCCACCGTGATCGCAAAACCACTTCAT
CACCGCAGCCGCGGCACCTTTGCGGATCCGAACGGATGCGCTC
:::::::::::::::Rv40T7.seq:::::::::::::::
TTCGTTCGATGGCGCCGCCCCGGCTACGGTTTGACCTGTGGGTGTCGAATTGGGGTCAAATTCCGAGGTCGGCGCGTAAGAGTGGTCATCCTGCAGGGGGGCCGGG
GCCGAACTGCGCCGGCTCACACCGCGCAACACCGACCAGCTGCTGTTCGACGGCCTGCCCTGGGTATCCCGCGCGCATCAGGAGCACGACGAATTCGCCGAGCTGC
TGGCTTCCCGCGGTGCGGAAGTGCTGTTGCTGTCGGACCTGTTGACTGAGGCACTACATCACAGCGGGGCCGCCCGCATGCAGGGGATCGCCGCTGCCGTCGACGC
ACCGCGCTGGGACTGCCGCTGGCGCAAGAACTTTCGGCCCTACCTGCGTATCTCGACCCAAGCANGTTGGCGCATGTGCTGACGCCGGCATGACTTCAACGAACTC
CCNTCCGACACGCCGAACGAAGTGTCGTTGGTGTTGCGTATGC
Clone Rv412
:::::::::::::::Rv412SP6.seq:::::::::::::::
GCGGCGAGTGTGGTGGGTGCCGAACACGAATCCAACGACGCACTGGCGGAGAGATACCACTTGCTGTACTGGAAGCAGTGCTGATGATCTCCCGTGGAATGTGCCC
TCGCCGCCGTCTATCGAAAACAGTGGCATGCTGCG
:::::::::::::::Rv412T7.seq:::::::::::::::
CAACCGCGCTCGGCGCGTCTGGGCCTTCCGCCGGCTCCGCCGACAATTCTATCTCTGGATCAGCGGGGCTCTCCGGGCCGGCCTCCGCGAACTCAACAGGCCGCGC
CTTCCGGCCGAAACATTCCCTAGCCATATATGATCGCACCTCGATACACGATCTGGCGGCAACACCGCAAAGCGTCCGACGGGCCCCAACCTCCGCAATTCAGTAT
CCGGG
Clone Rv413
:::::::::::::::Rv413SP6.seq:::::::::::::::
GAAGGTCGGCGAAGGTGTGGCTGGNTGCCGATCACGAATCCAATGATGCAGTGGTCGGAAGATATTAGCCACTTGCTGTTCTGGAGACAGGTGCTGATGATCTCCC
GTGGAATCTCCCTCGACTCCGTCTATCGAAATCGTGAACA
:::::::::::::::Rv413T7.seq:::::::::::::::
TCCTGCGCTCTGGGCCATTCTCGGGTCTGCCGACAATTCTATCTCTGGATCTGTGGGGCTCTCTTGGCCGGCCTCNGCGATCTCTCTTCANGGCGCGCCTTCCCGG
AAACATTCCCTATCCATATATGATCGCACCTCTATACACCGTTGGCGGCAACACCGCAAAGTGTCTGTCG
Clone Rv414
:::::::::::::::Rv414SP6.seq:::::::::::::::
AGCTTTACGCTGGCGTATCAGCGTTGGGGCCGCTGCCATTTCGGTCGCCCAACGCGTTGCCAGCTCCCTGCGCTGTCAGGGGCTTGCGGCAAACTGGCCACCCCCA
ACAAACTTGGCTGAGCTTGATC
:::::::::::::::Rv414T7.seq:::::::::::::::
CTCTATCTGGCGTCACATTCGCAATCTTTAGATTGCAGATATCGATAAAATCACCCGCGCGACAAGACCGCCCATGTCATCCTTCGATGTTATTTCGCCGGCCTGG
GGAAAGCGCAACGACGTTGCCTACACGTTCCGCCGT
Clone Rv415
:::::::::::::::Rv415SP6.seq:::::::::::::::
AGCTTTNCCTTGCATCTGCACCCCGATCCACGTCAGCCACGTCGGCGTTCTCCACCAAGAAGTTGCGGGCATTCTCCTTGCCCTGCCGAGCTGCTCGCCCTCCGTA
GGTGAACCAGGCACCCGACTTGCGGATGAGGCCCTGATCCACACCCATGTCGATCAGCGAGCCCTCCCTGCTGATCCCTGCCGTAGAGGATGTCGAACTCGGCCCC
TGCTTGAAGGGGGGCGAACAGTTGTGCACGACAACCCTTCGGCGACGAGGGTGTGCAGTTCCTCGACCTCGAGGTCGAACGTTCGTGCCCCGCCGTTGGCAGCCCA
CTTCTCGGATCACGGAATAGCGGANTTCTTCCGCCAGCATGTCGTGCAGGAATTTGTCATCCAGGGCATCCGCGAGCGCCTGCACGCCG
:::::::::::::::Rv415T7.seq:::::::::::::::
ACTGTCNAGGGAATGCTTCGCAGCATCTACCTGCAGTCGCTTGTGCATAAGCGGACGGCCCNAACCTGTTCGTGTTCCGGGGACACCAGACGGGGGGAGCAGCAGT
ACGGCGAAAGGTTTGAGCGGAAAGGAGTTGCGCAAATCGGGCGCCCCCAACACCCGTCCGCAAGACGCGGTCAACGAACCTGTTCAGGGATCAGGGTCAGGCGACT
ACCTGCACTGAGAACAAGCGATCTGCTGATCTGCCAGAAGATGGACATGAATGTCCACGGCAAGCCTGATGGCCTGCCGCTCTTCCGGGAATGTTTGGC
Clone Rv416
:::::::::::::::Rv416SP6.seq:::::::::::::::
TGAATTATGATCCCGACACAACTGCATCANTTTAGCCGCGTCGNGATGCTATCCGCCGACGGTTTGGANCNGGTCCGTGTCGTTCGTGTTGATCTCACCCGAAGTT
CTGTCCGCCGCCGCCGGGGATCTAGCGAACGTGGGATCGACAATCAGCGCCGCCAACAAGGCGGCAGCGGCTGCGACCACGCAGGTGCTGGCGGGGGGGGCGGATN
AGGTGTCAGCGCGCATCGCGGCGCTGTTTGGTATGTACGGCCTGNAATATCCGGCGATCAGTGCGCAAGTTGCCGCGTATCACCANCAGTGCCGTGCAG
:::::::::::::::Rv416T7.seq:::::::::::::::
AACGGGGACCNCAAGAAACCATTCAANAACGAGGGGTCGTCACCAACGTCGAAACCGACGGTTGCCAGCCGGCCCACGATATTGCGTGCTCGAGGGTCCGCTGTAC
CCTCACCGAACGTGAGTCCCACACCGCGGAGGCGGGCGACTCTGGCGTCGTTAGCAGCCCGAGCTCAAGGTGTCCCGCACCACTGTCGGAATGCTTTTAACCGACC
GGATCAGCTCTCCGCCGATCTACGTGAACGAGTGCTTGCCACGGCCAAGCGACTGGGCTATGCCGGACCGGATCGGTGGCGCGATCGTTGCGGACCCCGCCCCGCC
GGTGCGGT
Clone Rv417
:::::::::::::::Rv417SP6.seq:::::::::::::::
AGCTTTGGAGCCNCNCCGANCCNCCGGTACGCCCCGCCACCGCCGTACCCGGCACCCGACCCCTTTGAGCCGTTCGCCGTGGCCGCGGTGGANCTGGCGACGAGGG
GACGATCGTGCTGGCAAAGTGGTCGATGGCACGCTGGCCGCCGATCTGAAGGTCGGCATGGAGATGGAGCTGACGACCATGCCGCTGTTCGCCGACNACGACGGGG
TGTGCAGCGCATCGTCTACGCGTGGCGGATCCCATCGCGCGCCGGCGACNATGCANAGCGCANCGATGCTGAGGAGCGGGGGGATGAGGATGAGCCCCCCGGGCAC
CCGTTTACMTCCTGGGTGCCGGTATGCACCCGTGGGGAAATGGGTAATGACTTC
:::::::::::::::Rv417T7.seq:::::::::::::::
TTCTCNCATCGTTCGTACTNNGATGGACGCTGCTGCCCCGAGGCGATCCTGGCCAACCGGCCTCTCGCCCCCCCGACCTTCGGGGGGAACCTGAACTTCTTTTTTC
CGATGGGCGCTTGGGCCAAACGTACCGGGGCTATCTTCATTCGGCGTCAGACGAAGATATTCCCGTCTACCGCTTCGTATTACGTGCTTACGCCCCCCCAGCTGGT
GCAAAACCATGTCAACCTCACCTGGTCGATCGAAGGGGTCGGACCAGAACGGGCAAGCTACGGCCACCGGTGTTCGGGATCCTGCGTTACATCACCGATGCGCGTC
GACGAAATCGACGGTCCCGAAGTGTATTGGTCCCCACCTCGATCGTGTACGAACAGCTGCACGAAGTGGAAGCCATGACCACCGAAGCCTATGGCCGGTGAA
Clone Rv418
:::::::::::::::Rv418SP6.seq:::::::::::::::
TTCTTCCGGGTACCGCTGATCGGCGGCACCATCACGCACCCCGGTGCAGGGCGAGGCGGCCCGCCGGTGTGGTGTTGCTACGGCCGGCCAGCCCGTACCGGTGTGA
TCGCCGGTGGTGCGGCCCGCGCGGTGCTGGAATGTGCGGGGTGCACGACATCTTGGCCAAGTCGCTGGGCAGTGACAACGCGATCAATGTGGTGCACGCCACCCGT
GGCCGCGCTCAAGCTGCTGCACCGTCCGGAGGAGGTGGCCGGCGCGCCGCGGTTTGCCAATAGAAGACGTCCCCCCCGGCCGGGATGCTG
:::::::::::::::Rv418T7.seq:::::::::::::::
GTCGAAAGTGACCATCTCTACCTTGAGTGCCATACCGCCCCGACCCTATGCCTCGGATAGCTCGGCGGAAAGAAACGCTTGCAGTGCCGCCCGAATAGGCCTACGT
CGTGAGCGCCCATCAACTCTCGCGCGGAGTGCATCGCCAGCTGGGCGGCGCCGACGTCGACCGTGGGATTCCGGTGCGCGCCGCGGCCAACGGCCCCGATCGTCGA
CCCGCACCGGCAGATCGGCGCGATGTTCGTAACGCTGCATAGGCACTCCCGCGCGCTGGCAGGCCAGTTGCGAAACGCCCCCCGCGGTGCCTTCCGTCGGTTGGCT
TTACCGCAAATTTGGGGTTGCCCCT
Clone Rv419
:::::::::::::::Rv419SP6.seq:::::::::::::::
AAAGCCACGGAAACGATTGCCTACTGCCGAATCGGGGAACGGTCCTCGCACACCTGGTTCGTGTTGCGGGAATTACTCGGACACCAAAACGTCAAGAACTACGACG
GCAGTTGGACAGAATACGGCTCCCTGGTGGGCGCCCCGATCGAGTTGGGAAACTGATATGTGCTCTGGACCCAAGCAAGGACTGACATTGCCGGCCAGCGTCTACC
TGGAAAA
:::::::::::::::Rv419T7.seq:::::::::::::::
TTTCGCCACCGCNAGGTCGTGCGCGTTCCAGAAAAGCGTGGTTCGGCCGGGCGCGAGGATTGGACGGTCCAANCTGACCAGCCGGTCCGGCCACCCGTTAGGCAGA
TCGCGGTGTGTATATGTTCGGCCCTCGGCATAAACGCCATTGCTGCGGTGAAAATCGGACATCTCGCCGATTGCCAGTCTACATGATCCGCCTTGTCCCCCCCCCG
GTCGTTGACAAACGCGATGTCNGCCTCCTGGGAAGCGGTGGC
Clone Rv41
:::::::::::::::Rv41SP6.seq:::::::::::::::
TCGCCAAGTGGATTCGTGCTCACCNACGAGATCCGTGGTCGGATCCGCNGCTGCGCGGGCTGCGACCCTGCATCTCGGCGGCACCCGTGACCAAATGGCCGCGCCG
CGAAGCAGACGTCTCGGCGGGAGCGGACGCCGACTGGGCGATGGTGCTGGCCGCGTGTCCCNGTCNCCCGACCCCCCCCATCNACCAAAACCGGGGGGGGGTCCCG
TTCTGGACCTATCCCACGTGCCNTCGGGGTCCACGCTCGACGCGACCGANAACGTAACCAGCGTCCTCGANCGGTCGCCCCCCCCCTTCCGTGACATGCTGGTGGC
GGCCGCGCCGT
:::::::::::::::Rv41T7.seq:::::::::::::::
GTACCGTCACCATGATCGCCCCCCATCGGCATCGGTGAGCTGATAGATCCCAGCCGGTTTCGCCAACCCCCGAGCGATCTTGGCGGCTGCTNGTNGTCGNTGANAC
NTAGCCACCAACAGAGCCCGGTGTGCGACAAGNGACTGATCGGATCTCTCCGGACACNTTCGAGGGGGGTCNTCAGGAGNCCGGGCGCCACCCCGAGGTAACCTCC
GCCCAGCCTCACACCGCGACCGGGTATCNCAAGTCGCGCAATANCCCACCACCTCCTCGGACCCCCACGTTGTATGCGGCTGGT
Clone Rv42
:::::::::::::::Rv42SP6.seq:::::::::::::::
ATACTCAAGCTTAGACCTCACTGATGTGGCGGGACGCGGGAGATAACCGCGGTTCGAGCCGTTCAACAGTGGTGTTCCCACACCAGTTGTTGCCTTTGCCCGAAGT
AAAGCGATTCGATTTGCTCGAAAAGAGGGCTGGCTGCTCGTGAGGGACATCCATGGCCGATACCTCAGCGATCTCAACGGTCAAGCGACTGCATGTTTGGCGCAAG
GTATCGCTAAGCATAGGTTCGTGACGGATTTGACAGCAAGAGCTTTCCAAAGATTGCTGTCCACATANTGATCGCATCTCTACACCTCTTCGCCGGTCGCTGTCAA
GAGCCATTCGAATCAGTTATCTCGCTCGTGCTTGGAAAAAATTTCCAGCCTGCGTTGGACAAACCGCGTCGCCAAAGCGGT
:::::::::::::::Rv42T7.seq:::::::::::::::
AGCTTCCCGAGAAACAGTGCATTCCCAAGCAGCCCGTTGTCACGCCGATGAGTGAAGAGTGCACGCAATCGCCGGAATCCGGCAAAGCCCCTGCACAAGCGAAATC
AACCCGGAGGCTGACAAGGCAACGTCGGTGATCCGTACCGCCTGGTTGGACAAACGGCAGAAGGCGGCCTCGTCCGGTCCATCTACGCCGAGCACACTGGTGATAG
CGCGCATCGGCATCGGTGCGGCCAGCCTGGAGACGACGTCCGCGGGCGTCTGGGTCAGTAACCCCCCCACCAGTTCTCGGGGGCAAGCTCCTCGAACCATCGGGGA
CGTCTCCAACGCGCCACGCGCCATACCTGGTGCCAGTTGCTTGCGCATCCGGGTGTGCGGCCGGATCGGACGTCGCAGAAACGCAGCCACCCCCCCGTGAGAGTGA
CCCACGGCGCTGGACACGTGTCTGGTTAC
Clone Rv43
:::::::::::::::Rv43SP6.seq:::::::::::::::
CGGCCGGGATGTGCGCAATGGCAGGTTGTCGCCCGGCTTGATGTCGGCGTTAGCGCCGGATTCCACCACATCCCTGCGAAAGTCCGTTGGCGGTGCAATGATGTAN
CGCTTCTCCCCATCGAGATAGTGGAGCAACGCAATCCGTGCGGTACGGTTCGGGTCGTACTCGATGTGCGCGACCTTGGCGTTGACACCATCTTTGTCATGGGGGC
GAAAGTCGATCATCCGGTAAGCGCGCTTATGACCGCCGCCTTTGTGCCNGGTGGTAATCCGGCCATGCGCGTTGCGTCCACCGCGACCGTGCAGCCCCCCACCCAG
CGACNTCTCCGGGGTTGACCGGGTGATCTCGGCGAAATCAGATACGCTGGCGCCGCGACGACCAGGCGTCGTGGCTTGTACTTGCGAAATTGCCATGGCTCTTTCA
GGTCTTTCTCTCACCTCTCGTCGCCGGGCTAGGGCGCATTGCCTGCTCCT
:::::::::::::::Rv43T7.seq:::::::::::::::
TAGCGGTGTAACCAACTCCCGGTCACCACCCGCAAACCTCTTGCGGCAACAGCACCGTCGACGCGTCAACCGGGCTGCCCGGAATCCTGTGGATGGGGCATCGAGT
GCATGGTCACGACGTCCCCGACGCGGCCGGTGGCAACGACAAGTGGCCCCGATGCACCACAAATGACGGCCCGCACACCGGTGGGGACGGCCAGCACGAGAGCCTG
TCGCCGAAGTCGACGCTAATGCCGTAGGCATTGGCCGTCACAACAGCGCGACGCCCCCGCGTACCACCGAGTCCAGGNGGTTGGCGGTCTCCTCGGCCAACCAGGC
GTGAACCCGGCGGATCCGAATGCGCAAGACCCGTGGGC
Clone Rv44
:::::::::::::::Rv44-2NDSP6.seq:::::::::::::::
CCATTGGTCGGTGTGCGCATACCANTACNACGCGCCCGGGCACCTGACGCCGGCGGCCGCAACCATTCGGTGGCCATCGCCATCGTCTGCCACCCGGTCACCACGC
ACCTTCTCCTGGCCGACCTAGTGCCGCCCACCGCCGCCGTTGCGTCCCATCGATCCGGTCAACATGAGCAGCGCCAACACCGAGCGGTACATGACATCTGCTGTGG
AACCAGTGACANATTCCGCCGCCCATGATGATCNTCGACCGTCCTCCGGATTCGGTC
:::::::::::::::Rv44-2NDT7.seq:::::::::::::::
GCCGGCCTGGTCAAAGGGGCGTCCGAAGGANCCGGGCTGGGTAACAAGTTCCTGGCTCATATCCGCGAATGCGACGCCATTTGTCAGGTGGTGCGGGTGTTCGTCG
ACGACNACGTGACTCATGTCACCGGACGGGTCGATCCCCAGTCCGACATTGAGGTCGTCGAGACCCGAGCTGATCCTGGCAGATCTGCAAACCCTGGAGCGCGGAC
GGGCCGGCTGGAGAANGAAGCNGCACCAACAGGCGCGCAAGCCGGTCTACGACCCCGGC
Clone Rv45
:::::::::::::::Rv45SP6.seq:::::::::::::::
GATCCACTGACCACGATGACATATCGAAATGCTCGACGATTCCGATGGCGATCAAGGCCACGATGCCCTGGCGTTGGGCGGTATCTGGTGGATGGTGTACCCCCGG
GTAGGTTCCCGTGATCGTGTCGACCCAGTCCACGCGATGGGCGGCGAGGTCGTCGGGCACCGCATCAGGGGGTNTGGGGGAGTGGGGGTCGAGTTGGGGGGGGGGG
AGCTCTCCCCCGGTAGAACTCTCACCGTTGGTCGCCGCGATCTTCTCTANCGTCGCCGCGTGGTCAGGAGGTAANCAGCTCACCGGTTCGGGCCCCGTCGTCCCCC
GGGCATGAACGCATCTGCGAATCCGGGCTGGGATGCGAACAACGGACCTGTGCCG
:::::::::::::::Rv45T7.seq:::::::::::::::
TCTACTGCCGAATCGGGGAACGGTCCTCGCCCACCNGGTTCGTGTTGCCGGAATTACTCAGGCACCGGAAACGTCGAGAACTACGAGCGGAGTTGGACANAATACC
GCTCCCNGGTGGGCGCCCCCATCGANTTGGGAAGCNGAAATGTGCTCTGGACCCCACCCAAGAATGACATTGCCGGCCGCCCTCCAACTGGAAATAGAANCNGTGA
TCACCCGCCGCGTTCTTGGAAGGAATGGCATGCCCTGGGCCGGCGTTCCTTCCGCTGCCGACTCCTCCCACCAATTCACGCCGAAGGGCGTCCGTCTGC
Clone Rv46
:::::::::::::::Rv46SP6.seq:::::::::::::::
ATACTCAAGCTTCTGTCACCGAAATCCCGCATGGGATAACGGGTTTAGATTTCGACAACGGGACCGTGTTTCTCAACAAGCCGTCATCAGCTGGGCCCGCGGACAA
CGGTATCTACTTCACCCGCTTTCGCCCGT
:::::::::::::::Rv46T7.seq:::::::::::::::
CTGGCTCAAGCGCTCGGCGCGCAGGTGAACTCGGACCGGCTCGACGTCGCCGAACGCAAGCCCTGCTGGCCCACGGACCCGTCGTCATATGGGGGGAGGGGGGGGG
TGCACAAGTCTACAACAACGCCGGCATCGCGTACAACGGCAACGTCGACAAGTCGGAGTTCAAGGACATCGAGCCGCATCATGCACGTCGACGTTGGGCGGTCCTC
CACCGGGCCC
Clone Rv47
:::::::::::::::Rv47SP6.seq:::::::::::::::
CCGCCCTCCGCATTATGGGTCAAGAACCATCGGGTCGGACTTCTGGGCTTCCAACGCTCGCGCCGTCCCN
:::::::::::::::Rv47T7.seq:::::::::::::::
CCGTGGCACTGTCAGCATATGCGCCGCTCCTCCTCATCGCTGCGCTCGGCATGCTGCCCCCCCCTCATGGCGTCACCCTACCCCAGCCGAACCGGAAAACGAGAAC
GTGTTCCATTATTAGGGTGTGAGCACCAATACCAGATTGCTCACCAGGAACTCACGGCAGCACCGGGACCGGATGTCGGGCCAGAGCCCATCTCCCCTGGTAGGGG
GAAATACCGCTAACGCGGCTCCGGTGCCG
Clone Rv48
:::::::::::::::Rv48SP6.seq:::::::::::::::
TACTCAAGCTTGTCCAAATATCGAAGCGTCGGGTCGCGAGGCTCGGTCGGCAGCTCCAGCAAAACCCGCTCCACCCCTAGATGCCCGGTATCCTCAAGGTCTTTAT
CCGCCGCTTCACCCCACTGGCACACGGTCACCGGCACGTCGCCCCCGGCCATGGCGCAACCGCTGAAGCGGACCCCGACAGCCCCTGCCCTGATGGACTGATCGGG
GATCCACCCCGGCATTGAGCCGGGCTATCCGCGGGAAGTTCGCCGGTCCCCCCGCCCACATACAGCGGAGGATAGGGCTTGTCACCGGCTCGGCAGCAGTAGTCCC
GGATCGAAGTCCACATATGTCCCATGGAATTCCGCCTGCTCCTGCGTTCAGATCTCGATTATCGCGCGCAACCGCTCATCGATCACACTGCCCCCACCCCCAGGGT
CCACACCATGGTTGGCGACTTCTTCGCGCAACCAGCCACACCCACGCCGAAACGAAACCGTCCCTGCG
:::::::::::::::Rv48T7.seq:::::::::::::::
CAGCCATGCAAGCTTGGCCAACTCCTCATCGGACTTGAAGGTGCCGTCCTCGTTGGCGGCCCTGCTCCACGGCAGTTGATGGCACCAGGAATGTGTCCGGGGGGGG
TGGCTTTGTTCCTGCGGCAGGTGCGCGGGGGCCAGGATCTTGCCGAGAACTCGTCGGGAGAGCGCAGTCGATGAGGTTCTTGAGCTTGATGGCCCCCCCCCCACCT
CGTCGCGGAATGCCCGAATCGTGTTATCCGGCGGGANGCGGTGTAGGAAGTCACCGGCCGGCTGACCGGGTCGCTGGACAGGCCGGCTCCGTCGAGCTCC
Clone Rv49
:::::::::::::::Rv49SP6.seq:::::::::::::::
ATACTCAAGCTTCAAAACAGGCCTGTTGTGGGCGACCCGGCTCGCCGAGTTCTCGACGCAACCCCCTCAACTGCCCCCCACCCCCCCCCATCTCCCCGGTCACGCA
GGGGCCGCGGCCCGCGCCGCAGCGACGGCGTGTTCGCGCAGTTCGCCGTCAATGATGCTGACCTGATCGGGCCACCCCCCCTCTCCCCCTCGTCCCGTTCACTAAT
CGCCGGTGCTCAGCAGCGTCTCGACAGCCACCACCCGAGTGGAGACCAGATGCNCCACCACGGACCCGCAGCGATGCCGATCACCTCACCCCGTCC
:::::::::::::::Rv49T7.seq:::::::::::::::
CAGGCATGCAAGCTTTGCAGTTGCTGAGTAATGTCGGCCAACGTCACCACAATCGCGATGAATTCAATCATGCCGCCCAGGCGGGCCAACCCAATGGTGGCCGGGA
GCGGCAGCTCGATCGCAGCGCGGAGGTTGCCGGCCGCCAGTTGATTCACGAACAGGGTGAGGTCATAGGCGGGCAGGATAGTGACGAAGGCAAGACCTAGATCTGC
CGTCGGAAGAAGAATCGAGTATCCGGTCGACACAACGGAAGCGAAAGTGCCGCGATGTTGATGAGCGTCGCCGGTTGTGGCGGCGGTGGCGGCGGTAGCACCGGTC
CGCACATACCGCGGGCAACGCGGGCATCCGAATTTGGGGCAGGGTGTTCAAGGCGGCTGGCAACTCACCATGAATCT
Clone Rv4
:::::::::::::::Rv4SP6.seq:::::::::::::::
CCGGCTCGTATGTTGTGTGGAATTGTGACCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACACTATAGAATACTCA
AGCTTGGCCGCAGGGCCGAGTCGATTGGTCGCGGTCGCCTCGACAGTTAGCTTATGCAATGCTAACTTCGGGGCAAGTTCAGGCGGATCGGCCGATGGCGGGGCGT
AGGTGAAGGAGACAGCGGAGGCGTGGAGCGTGATGACATTGGCATGGTGGCCGCTTCCCCCGTCGCGTCTCGGGTAAATGGCAAGGTAGCGCTGAGCTCGGGGGGT
CGATTTGCCACCTGCTGCCGTGCCTGGGCATCGCGGTTTACCAGCGTAAACGTCCGCCGGACCTGGCTGCCGCCCGGTCTGGTTTCGCCGGCGTGACCCCCGGTCG
CCATGACAGTGCGACCCTGNACCGGGCTGGCC
:::::::::::::::Rv4T7.seq:::::::::::::::
GTGTGCTGTCAATTCAGAGCTGAGCCTGATGCACTCAACTTACTGAGCATGCTAACGCTGGTCGTGCGGGTCTTGTTCCCGCGTGTCGGCAGGGCACACGCTCGGG
GCGTAGCTGGGAGAGGCCCCGGTCAAGCCCGAGAGCAGTGCTCAGTCCGCCAGCTTGACCGACTTTCGATGAGAACGCGTTCTCGCCGTATTGAAGGGGGCGTTCG
TGAGCCTGCGCGAGCAGCGCTCGCCGAGTGCGGCCGCTGATTCTTTCATCGAGCCAGGAGGCGCATTCGTGCTCGGGGGTGGGGGTGGGGGGGGGATCGTCGACGC
GATCCGTCACCCACTCCTCGATCAGGTCTGCCTCATCGAACGGGCCAACGGTGCTGTCGGAGTAAGTGTGCGTGGGCACCGAGCCCCTGCTGTGGTACACCCCCAC
CGTTGCATGAACAA
Clone Rv50
:::::::::::::::Rv50SP6.seq:::::::::::::::
ATACTCAAGCTTCACCAGGCGCCCGGCGGGCGGGGGGGGAAGCCAGGCAGCCCCCTCGGGGGGTCGGGGGGTTGGGGGGGTCCCCCCCAGCAGTTCGATCTCTGGA
TCGGCGGGGCTCTCCGGGCCGGCCTCGGCGACCTCAGCGGCCCGCGCTTCCCCCCCAACCAATTCCTAAGGGATAGATAACCCCCTCAAATGCAGGTTGGGGGGGG
CAACCCGG
:::::::::::::::Rv50T7.seq:::::::::::::::
AGCTTCCGTCACGACCCGCCCTCGCCGGTGCCGGCGCCATCGGTCATCGGATCTCATGACGACGTCACGTAGGGCCCCCTAGCGGGGGGGGGGGGGGTCAACTGGC
GAGGCGGCGGCGACGTGACTGAGCTGGCCGAGCTGGACCGGTTCACCCCGAACTACCGTTCTCGTCGACGACTTTCAGCAGGGGGGTTGCAGCCCCCTGGAAGGCG
CCGGCCACGGTGTTGCTGGTGTGCGCGCCGACCGGCGCTGGCAAGACGGTGGTCG
Clone Rv51
:::::::::::::::Rv51SP6.seq:::::::::::::::
ATACTCAAGCTTGCCGGGACCGCGGAACAGAACCGGCGGTTCCTACCGCGGTGTGCGGCCGGCGGATATCGGGCTCCCCCATAACCGAACCCCGATGTGGCGCTCC
:::::::::::::::Rv51T7.seq:::::::::::::::
ACGTTGGCTCTGCCGGAACGTATTTCCAGCGGCACGCATTCGGCGTGGGTGCCGGGCGGGATTGCGTCGCTGGGATCACCAGCAGTCCCCCCCCCCCCCTCCCCTC
GGGCTATGAATTGCACCGAGCCGGAAAATCCNCAC
Clone Rv52
:::::::::::::::Rv52SP6.seq:::::::::::::::
ATACTCAAGCTTGTCGTATTCCGTGGCACTGTCAGACATATGCGCCGCTCCTCCTCATCGCTGCGCTCGGCATCGTCGCCGGCGGTCATGGCGTCACCCTACCCAA
GCCGAACGCGAAACGAGAACGTGTTCCATTATTAGGGTGTGAGCCAAATACCGATTGCTGAGGGGGGTCAGGGGGGGTTGTGCCCCCCCCCCCCCACCCCCCCCCC
CCATCTGGGGTGGTAGCGGGGGA
:::::::::::::::Rv52T7.seq:::::::::::::::
CGTTGGTAGCCCGATATGCATAGTGTATCTTACTGAACATGATTTTCCATTATGGAGCCCGGGTGCCCCACCCCAGGTGCCCCCTCAGCCCCCCCCCCCCCCCACT
GACCAGGTGTTGCGGGCGAACATCGGCCCCGGCTTCGGATTCCGGTCCGGGTACCGGGCGACCCACCGCTTCGAAGGTA
Clone Rv53
:::::::::::::::Rv53SP6.seq:::::::::::::::
ATACTCAAGCTTGGCCAACTCCTCATCGGACTTGAAGGTGCCGTCCTCGTTGGCGGCCCTGCTCCACGGGCAGTTGATGGCACCCGGGTTGTTCCCGGGGCGGTGG
CTTTTGTTCCTGCGGCAGGTGCGCGGGGGGCCATGATCTTGCCGGAAAACTCGTCGGAGAGCGCACGTCGATGAGGCTTCTTGACGTTGATGGCCCAGGACCTCGT
CGCGGAATGCCCGAATCGTGTTATCCGCGGGGAGGCGGTGTATGAGGTCACCGGCCGGCTGACCGGGTCCCTGGACAGCGGGCGTCCGTCCAGCTCCCCACTTCTT
GCGGGCGCCGTCCAACNACTTGACTTCTCCTGG
:::::::::::::::Rv53T7.seq:::::::::::::::
ATATCTTAAGCGTCGGGTCCCGAGGCTCGGTCGGCAGCTCCAGCAAAACCGCTCCACCCCTAGATGCCGGTATCCCTCAAGGTCTTTAGCGGGGTTCACCCCCCCA
CTGGCACACCGGTCACCGGCACGTCGCCCCGGCATGGCGCAACCGCTGAAGCGCAACCCCGACAGCCCCCCTGCGGTGATGGACTGATGGGTCCCCCCCCCCCTTG
AGCCGGGCTATCCGCGGGAAGTTCGCCGGTCCCCCGCCCACATACAGCGGAGGATAGGCTTTGTCACCGGCTTCGGCCAGCAGTAGATCGGATCGAAGTCCCACAT
ATGTCCCATGGAATTCCGCCTGCTCCTGCGTCCAGATCTCGATTATCGCGCGCAACCGCTCATCGATCACACGTCCGCCACCCGAAATCCCACCCCACATGGTGGC
GACTTCTTCGCGCA
Clone Rv54
:::::::::::::::Rv54SP6.seq:::::::::::::::
ATACTCAAGCTTGTCGCGGTAAACCCGCGAGCAGGGCGGTGGGTGCGGTGCAAAACAACCACACTTCTTTGCGGGTCGGTGATCTGCAGGGGGAGGGGGGGGGGGA
CCACCATGCGCGCTAAATCGGCGATCAGCGCGTCGGCTATCGCCTGGTGCCGCCCACCGGAATCGGCCAGCCGACCGAATGGCCAGCGTTGCCAGCCCCATCAGTC
CGGCGCCGGCCGACACCAGTGACGGCAACGGTGAAATCGCGTGGGCGGCAACGCCGGTGAACAACGCGCGGGGCATCCTGGCCCCCAGCGACCCCCAGGGTTTGGT
GCCCTGGGGCCAGCATCCGCAGCCCGAGACGCAGGACCGAGCCCAGTGCAGTAGGCAAAGACCGCTTGTCGGAGACATGAACTCCACGACCGT
:::::::::::::::Rv54T7.seq:::::::::::::::
AGCTTATTGAACCGCGGGTCGCAGGCAAAGTGGACCTCATAACGACTCGGGTCCAGCGACCGCGCCCAACACGAACGGCCGGAACGACGTGGGGAGGGGTGCCCCT
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CTCCATACTCGCCCCTAATCTCGAGGCAGCCCGTACCCGCAGGCAACCTCCAAAAAATGCAATCCCCAAAATTCAATGCGTCGAGCTATTTCTCACACCGACCCCG
CTAGTTGCGGATCAGAAATCCGTTGGGCGCGGAAGTCCAGCCGATTTGTTCTCCCGCTCCGCATCATGCTTGTAATCGTTGGAAATTCATCCTCATATGCCCCTGC
ATCGCTTCATAGGGTCCAGGCCAAACCGGGCA
Clone Rv55
:::::::::::::::Rv55SP6.seq:::::::::::::::
CTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGGGAAGCTATTTAGTGACACTATAGAATAC
TCAAGCTTGGCCACTCCGCGGTGTGTGGTGGACCCATCTGAGCATGTGCCAAAACCGGGGCAGACAGTCCCAATTGACGTGAGCCCCCCCTCACTTGCTGGGGTAG
CGTCG
:::::::::::::::Rv55T7.seq:::::::::::::::
TAGCGCCCCTCCCGGGCCGGAGCTCCACGGCGTGGATCAAGGTACCGGCCCGGGATGTTGCCATGGAGTTGTTGCCCCTTGATGTCCCCTTACCCCCCCCCCCCCA
TTCCACCACATCCCCTTGCGAAAGTCCGTTGGGTGCAATGATGTAGCGCTTCTCCCATCGAGATAGTGGAGCAACGCAATCCGTCCCCTACCCCTTCGGGGTCTAC
TCCCCCGATGTGCCGCACCTTGGCGTTGACACCATCTTGTCATTGCGGCAGGGGGATCATCCGGGTAAAATTATGAGGGGGGGTTGTGCCCCTATTAACCCCCCCC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
ACCAAGCGTCGTGGGCTTGTTCTTGCGAAATTGCATGTCTAATCAGGTCTTTCTC
Clone Rv56
:::::::::::::::Rv 56SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TTCCGGGTTGACAAGCAGGCCAACCTGCACTTCTC
:::::::::::::::Rv56T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TTGTACTTGCGAATTGCCATGTCTAATCAGGTCTTTCTC
Clone Rv57
:::::::::::::::Rv57SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
AAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
:::::::::::::::Rv57T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CACCACCACCTCCGCCGGCCCTCGCTGAGGTCC
Clone Rv58
:::::::::::::::Rv58SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CGCGCTGCTGTTCGCGCTGTCGGCCAGCTCGCAGGACGCGCTGCGGCAAACCGCCGCGCCCGTGGGATTGGGTCT
:::::::::::::::Rv58T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CTCGCGGCCGGTGGTGGCCTGGAATGTCCANCGCCGTTCACCTACGTGACCTGATGGATCCGGGGGGNT
Clone Rv59
:::::::::::::::Rv59SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
ACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
:::::::::::::::Rv59T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CGTGACGGGTACTTGGCGCGATAACGTGGTG
Clone Rv5
:::::::::::::::Rv5SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GTCGCGGGCGCCACCGAGGTCGTTGACCACGA
:::::::::::::::Rv5T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TCCGATCCCCGCGGCTTCAGG
Clone Rv60
:::::::::::::::Rv60SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GATGCCGACCGAAATACGACCCACATGGCTCTTGTTGCNCAGTGTTGGCGGCATCAAATACCCTCAGTGCCGTCCGAC
:::::::::::::::Rv60T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TCGCGCACTTCGCGATCGCGTGACTGGTCCTCGCGATGCCCCTGCGTAGC
Clone Rv61
:::::::::::::::Rv61SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
:::::::::::::::Rv61T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
ATAGCTCGACCGGATCGGAGAGAATCTGGTTCTCGT
Clone Rv62
:::::::::::::::Rv62SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCGAACGGTTGCCAGCCCCGGC
:::::::::::::::Rv62T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
ACCGATCTTTTCGTCGAAAATGGCGGTCGTGTCGGGGGTATACGTCCGACATCCCCAGCCCCTCCCGCTGAGGTGA
Clone Rv63
:::::::::::::::Rv63SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GGGCGGGCAACCGTCCGCTCAAGTCGCTTTCCGATCTGCTCA
:::::::::::::::Rv63T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
AATCAGAAACCTGGCGCGCGACACAGCGTGTACTTGC
Clone Rv64
:::::::::::::::Rv64ZP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CGGGAGTTGCTCGTCGGCCGACCGACCCGGCCAGCCGGGGCAAAACCGGGGAGATTCAGGGGTGGGTAGGGGTCTGGGGGGGGTGG
:::::::::::::::Rv64T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TCTCTCGATGCGGTCTCCACCACCGAACAG
Clone Rv65
:::::::::::::::Rv65SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GGTCCACTCACGGTACCGGGTATTTTATCGTCGACGAGGGGCACATGGT
:::::::::::::::Rv65T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GCAGCCCAATGAGTTGCCGCGCGGGCTGCATCGCGGCCGGACGAGG
Clone Rv66
:::::::::::::::Rv66SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
ATTGCTCGTCGGCCGACCGACCGGCCCAGCCGGGGCGGCAAAACCCCGGAAGA
:::::::::::::::Rv66T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CAATCGCGGCTCACTTGNGCGATNGTCNAACAACCAACGCGNCCTGGTCACCCTCAAAGAAAATGGGTGT
Clone Rv67
:::::::::::::::Rv67SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCGGCGGGGCT
:::::::::::::::Rv67T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GCTGAGCCGCAGCTGATCCGGGCTCGCCGCGGCGTGATCCTGGCCTGTGGTGGTTTCGAGCATAACGAGCAGATGCGAAT
Clone Rv68
:::::::::::::::Rv68SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CATTTCAGCCCCCACACCCCCAGACTTGCTCTCGATGNCCCCG
:::::::::::::::Rv68T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TTTGGACCGAACGGGATTCATACTCCGCAGGCCGGGGCCCCTCCGAAAATCGGCACATGTCCTTTGATCGTTCGCAACAN
Clone Rv69
:::::::::::::::Rv69T7D3.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CGATCGTCACAAAAAATCTCCGTCCT
Clone Rv6
:::::::::::::::Rv6SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CGGTAGGCACCCCAATCGACCCCTACCCCGGCTATCTCACCCCCGATCTCCAGGCTCCGCCGATCCATGCGCATCCGGTCCGGATCCC
:::::::::::::::Rv6T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TTGTTCTTTTGCCGGATGGCCGTGTTTGCTGACATATCGGCGCGCCGCCCCCCCCCCCC
Clone Rv70
:::::::::::::::Rv70SP6D2.seq:::::::::::::::
NCTACGCTGCTGAATGTTGTGCGCCGGAGGANCTCAAGACCCACGCGGTTGTACGCCGGACNTGGCGACTGGTCAGGGGGGA
:::::::::::::::Rv70T7D3.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
C
Clone Rv71
:::::::::::::::Rv71SP6.seq:::::::::::::::
CTAAGCTTTCGGGTCCGCCGCCACTAGTACCGCGTTGCCGGCCCCCCGCCGACCTAGAATGTTCCGCCCCATTGCCGTTCCTCCCCGCCCCCCGGTT
:::::::::::::::Rv71T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TGTTCCGCATACGATT
Clone Rv72
:::::::::::::::Rv72SP6D2.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
:::::::::::::::Rv72TD3.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TTGCGCTGGG
Clone Rv73
:::::::::::::::Rv73SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GCCACAAACGCGCCCCCGGTATAC
:::::::::::::::Rv73T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CGCACCGCGATCGGTCTGTCGCGGGCATGTCCAAACAACTGAAATCCCGTCAACATGGTCGAC
Clone Rv74
:::::::::::::::Rv74SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
ACGTACCTCCGACGGCCAGCTGACGAACGGGTTTTGACGGA
:::::::::::::::Rv74T7D3.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GGNACACTNCGGTTCACGTTAATCANCATCGCGANTTNCTNCGTNTTCGATTANTTCTGCTAACGNNTCNNNNGTCCCCTGGGGTAGAG
Clone Rv75
:::::::::::::::Rv74SP6D2.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
:::::::::::::::Rv75T7D3.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
ACTGGTCTAGCCACACCGGGGAGAATNCNCGACCCGGNGCTATCGACCCNATCACGGCTTGTCGNAAMGATAGNCAGCC
Clone Rv76
:::::::::::::::Rv76SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GGCGCCCCGCGAGTTCTAGCACAGCAACGCCGCGGCCACCACAGGGGGCG
:::::::::::::::Rv76T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCC
Clone Rv77
:::::::::::::::Rv77SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TTGGCGCCCCAACGCCCCCGGCAGCATCGACGTGGGTAGAGCTGGATGCCGCCAGCCACGGGGGGTGGGACAACCCCCCGAGCTGCGGGGGACCCCCCCG
:::::::::::::::Rv77T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
ACCCCGAAGGGCCGCTGGCCCGGCGGAACCTTGTCCTCCA
Clone Rv78
:::::::::::::::Rv78SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TGNTNGGATCNGCCGACGTGCANCTGAANGANTATCCCCGGGCTGTNCCACNAGGTGTTCAACGAACCGGANCGCAACCAAGTG
:::::::::::::::Rv78T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TGACA
Clone Rv79
:::::::::::::::Rv79SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GCCGTCCTTGAAGAAACCGGTGGCGGNGGCAACCC
:::::::::::::::Rv79T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
AACGGCACCANGCGCAACCGAAGGGCCAATCCGACATCGG
Clone Rv7
:::::::::::::::Rv7SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCTGTTGGCCGACAATCTCGGTGACTTCGCGGTCAAGTCACCCGGGCCGTTGCGGGTGTCCGCAGCCTTGTCCAGCCCCCTCGCTTACTCCA
:::::::::::::::Rv7T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TTCAGCCATCGGGGACATCATGCTCGCTTCATACTCCTCGACCAGTCGGCGGAACAGCTCGATTCCCGGAACGCCCACGCATGGTG
Clone Rv80
:::::::::::::::Rv80SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TCATCACCACCATCGCTAGC
:::::::::::::::Rv80T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TTCGCCGCCGACG
Clone 81
:::::::::::::::Rv81SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
:::::::::::::::Rv61T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCACAACCCGTCGCGATCCAGCTCAGCCACGATCTCCACAATGCGCCCATCAATCGCATTGCGCTGAACGGGCAACTCCGCCAACTCCTCCAA
Clone Rv82
:::::::::::::::Rv82SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GTTATTTTCCTCGCTTCCGGGCTGTCATCATTAAACTGTGCAA
Clone Rv83
:::::::::::::::Rv83SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GANTTCTCCNGGGTTGAACCGGTNATCT
:::::::::::::::Rv83T7.seq:::::::::::::::
TGTGTGTGGTGGTAACCCATCTGAGCAGTGTGCCAAACCGGGGCAGCCAGCTCCCCAATTGACGTGAGCCCCGCTCACTTGCTGGGTAAGCGTCG
Clone Rv84
:::::::::::::::Rv84SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCGACNAACGGCACTCCTTTTTTCCNTTTGCTCTTTC
:::::::::::::::Rv84T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GCCGTGGAAATATCAGCAATGATA
Clone Rv85
:::::::::::::::Rv85SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
AACACTCCGGCGGCGGCAGCGTGATCAACATCAGCTCCACCATGGGCCGGCTGGCGGCGGGGGGTTTC
:::::::::::::::Rv85T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
AGATCGCGCTGACCACACTCAACGCCCGGACGGCTGTCCCTACCGGCGATCCAACCGGAGT
Clone Rv86
:::::::::::::::Rv86SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCGAAATGCTGCGCAACATGCTCTAC
:::::::::::::::Rv86T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CGGGTCCGGCAGCNAACCCGCCCGCGCCGATACCCGCTGCCCCGCGTGCGTGATTGACCGCCGCGCGCACGCTGGCCANGGATCAAACGGGCT
Clone Rv87
:::::::::::::::Rv87SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TAGAATCTTTCCGCGGGCGGGTGAATCTCTGCNGGATCGGGGCNGGCGC
:::::::::::::::Rv87T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GATGGGCAGTCGCCGCTCGGCATCGTCAGCCTCGCCGC
Clone Rv88
:::::::::::::::Rv88SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
AGGGCTTCGTGCGGACCATCAACATCAAGTTCCAAGACGCCTACAACGCCNGTGGCGGCCCACAACCGCTGTTCGACTTCCCGG
:::::::::::::::Rv88T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TCCAGCACTCGGTCGATG
Clone Rv89
:::::::::::::::Rv89SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GATATTCNGCCNTCCCANCTCGAGCCTGACNCTNGATATCGTCGANNCTCACCATCNCGATCNGCTGTGCCGGTNTTGCTCGGACTN
:::::::::::::::Rv89T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
ATCGGCANACTCNCGTCGATGTGCCGCGGCGCAACCATCCCCCACAATGATCNGGTGCGTCTGATCAGGCN
Clone Rv8
:::::::::::::::Rv8SP6D.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TTGTGCGACCCTCAACGCCATTGCCGGC
:::::::::::::::Rv8T7D4.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GACACCGGCGAGACCACTTAGGCAGTGACGGCCT
Clone Rv90
:::::::::::::::Rv90SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TCGCCNATGGCTCCNGCGNNTCGCNCAACGTNTGGTTTGGTNANCTGCCTACCTGGTCNT
:::::::::::::::Rv90T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GTACCNTCATGTCGGTCTGCGCNCGATATTGACGATCGTGTTCCCACGANNNNNNGANCCTCATCACGCCGGTTCGAGTGCCG
Clone Rv91
:::::::::::::::Rv91T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
AANCGGCGCTNANNANATNCGCGNGAATGNCCGTCCACNANTGCATGG
:::::::::::::::Rv91T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TGCCAGCTCGACCTGACGCTGGAAATCG
Clone Rv92
:::::::::::::::Rv92SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GANCCCGAGCNATNCNANGTCCGTCAATC
:::::::::::::::Rv92T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CACATAAGACGCAAACTGCTCTATTGTCAANTCCCANCGTGGTGTGGCNCATGAAGATGTTTGG
Clone Rv94
:::::::::::::::Rv94SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GTTGGCANTNACCTCNGAGCCCCATTGGACATCANCCCANCCCGCCTGCGAACGGGAACGTCAGCNCNCTGGCGACAACCTGGCCAACAN
:::::::::::::::Rv94T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GCATGGCCGTCTGGGGTCANCTCNACNATTTCANCCGTTGACCCGGGGTCACA
Clone Rv95
:::::::::::::::Rv95SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GGCTCTACGCGCCATCTTCCCCGGATTCTTCGCGGCNGNGTTNCCGNGGGACCCCCGGACTGTGACNGGCCAACGGGCTCATCATCG
:::::::::::::::Rv95T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
TGTCTGCGANCTCGCGCACCAGCTCGACGGCCCGGGCA
Clone Rv96
:::::::::::::::Rv96SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GTAAGCAAAATACGT
Clone Rv9
:::::::::::::::Rv9SP6.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CGCCGCACCCGACCGCGG
:::::::::::::::Rv9T7.seq:::::::::::::::
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
CCCCTACAAACAGGATCCGTTGCCTGCGAGCGACAGGCTCCGGTGCGGCGTTGGCGCCCGTGCTCGTCCCAGCGTCCGGTCCCCCTCGGGGGACGCCCCTTGTTTC
GGCCGGCGCCGGCTGGTCGGCAAGGAAGACCCGTTCCGGTGCTGAT
TABLE 4
End sequences of the polynucleotide inserts cloned in the named
recombinant BAG vectors contained in the I-XXXX M. bovis strain
Pasteur genomic DNA libraiy.
RvXXXSP6 corresponds to the SP6 endsequence of the clone RvXXX;
RvXXXT7 corresponds to the T7 endsequence of the clone RvXXX.
RvXXXIS 1081 corresponds to a region located close to a copy of the IS1081
repetitive sequence (Insertion element)
The character << - >> denotes an uncertain base residue.
Clone X0001
::::::::::::::::X0001SP6.seq::::::::::::::::
AAG-
TCGGGTTTCCACACGCGCGGTTTGACCCTAGTCATATGTAATCATGTGTACCATGTGCGGGGCGCTTTTCGACGGC
CGCGAAGGACCGGA-ATTTCCTGTGATTTCACTGCATGCGTACCATCTGGCACAATTGAGCA-TTGTCT-TCGCGG
TGGTCGG-CGGGTTGCGTGCCGCCTGCTGCGA-ATGCACCA-TAAGCCCGAACCCACCGGCTTGGTGACCACCCGC
ACGCTGCGTGTGGGGGGTAACCACTCCGCGACCCCAAGGATGGTCATTTCCAATGAACCGGCTGGACTTCGTCC
A—A
::::::::::::::::X001T7.seq::::::::::::::::
GTCGCGGTTCGATCGACCCGATCTTCACCTCGTAACCTCGATGCTTAGCAGGATCCAGCTTGACCGCGTTTGGCT
CTACCCACTCTTTGAGTGGCGCCGTCGCCTGTGCCCCATCGGTGTTCATGACGAACGCTTCGAAAGACTTCCTCT
TGTGAGCCGGAATGTCTGCGTAAAGAAGTTCCATGTCCGGGAAGTAGACCCGGTCGCCCTCCACGTGGTACTCC
TTCGAGGTCCGCTTCTCGCCGGATCCGATAAACACCGGCCCCAGGCACCGCAGCGTGAGTTCGAACGGCTTCAG
GTAGGTGTTCATGCGGCGGACTCCGGGAGTGCGAGAAATAGCGGTCGCGCGTAGCTGTAGACCGGATGGTTTCC
GCCCAGGCTGACGTCGAAGATGCCTCCTTGGAAGGGGCGCGA
Clone X00002
::::::::::::::::X0002SP6.seq::::::::::::::::
AACTCAAGTTTTTACGGTGATCGCGCATCACCTGGTTCATGAACTGGAAGCAGCGCAGCGCTTCCTTTTCGGCCG
CAACATGAGCCAGCCTCTCGTCGGCGGTCGGGTGCAGGTGCTCGGGCAGCTCGGCCGCGACAGCCGCCTGACC
CTGAAACCAGCTTCCATATCCCGCGAC-
AACGACGCCAGTCCGCTACGTAACCCCTCCGCGACTGTCCATGGACAACAGCGCGTTTCTCCACCGACCGGGCCC
GGGTGT
::::::::::::::::X0002T7.seq::::::::::::::::
GTGCAGGTTTCGACAATGTGGTGCCGGTTCGGCGGCTACGTGCCATCGAGACACTGGCGCA-GCTATCGCACCC
GTTATCGGCTGCGAGCAAATCGCGGTATGCGTTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCA
CGACGGAAAGACGCAGATCGCCGTCAAGCATGTGTGCCGCGGATTATCAGGACTGACCTCCTGGCTGACCGGCA
TGTTTGGTCGCGATGCCTGGCGCCCGGCCGGCGTGGTCGTGGTCGGCTCGGATAGCGAGGTCAGCGAATTCTCG
TGGCAGCTCGAAAGGGTCCTGCCGGTGCCGGT
Clone X0003
::::::::::::::::X0003SP6.seq::::::::::::::::
TTCGAGTCATGCGCCCGCCTCGACCACGAA-ATGCACGTCG-GGTTCGATCGACCCGATCTTCACCTCGTAACCTC
GATGCTTAGCAGGATCCAGCTTGACCGCGTTTGGCTCTACCCACTCTTTGAGTGGCGCCGTCGCCTGTGCCCCAT
CGGTGTTCATGACGAACGCTTCGAAAGACTTCCTCTTGTGAGCCGGAATGTCTGCGTAAAGAAGTTCCATGTCC
GGGAAGTAGACCCGGTCGCCCTCCACGTGGTACTCCTTCGAGGTCCGCTTCTC
::::::::::::::::X0003T7.seq::::::::::::::::
GTCATGTGTACCATTTGCGGGCGCTTTTCGACGGCCGCGAAACACCGGAGATTTCCTGTGATTTCACTGCATGCG
TACCGTCTGGCACAATTGAGCAGTTGTCTGTCGCGGTGGTCGGCCGGGTTGCGTGCCGCCTGCTGCGAGATGCA
CCAATAAGCCCGAACCCACCGGCTTGGTGACCACCGCACCGCTGCGTGTGGGGGGTAACCACGCCGCGACCCCA
AGGATGGTCATTTCCAATGAACCGGCTGGACTTC-TCAACAA
Clone X0004
::::::::::::::::X0004T7.seq::::::::::::::::
AACAGCGCGGTTGAACTGATAGGTGCGGCCCGGCTCGAGCAGGCCGGGCCATTGTTCGATGCGGTTACCGAAA
GATCTCTTCGGTGACCTGCCCGCCGCCGGCCAGCTCGGCCCAGTGCCCGGCGTTGGCCGCCGCGGCGACGATCT
TGGCGTCCACGGTGGTCGGGG
Clone X0006
::::::::::::::::X0006T7.seq::::::::::::::::
GCATCTGGGCTGGCGGTGGTTCGCCGCTCCGAAGCCGTCGAACACCATCGCCAGCGCGGCTTCCACATCAACGA
CCATTTCGGCCAGCTTGCGGCGCATCAGCGGCTTGTCGATGAGCGCCCCACCGAATGCCCGCCGCTGCCCGGCG
TA-CACAGCGATTCGACCAGCGCGCGGCGCGCGTTGCCGAGGGCGAACGAAGCGGTGCCCAACCGCAATCTGTT
GGTCAGCTCCATCATGCGGGTGAGTCCCTTGCCG
Clone X0007
::::::::::::::::X0007SP6.seq::::::::::::::::
ATCGGTTTCCAGCAACAGCCGATCGACGGCTTCGCCCA-GGCCGCTCCCGGGCGACCCGACCATTGCGTGCGCC
GCGTAACGCCATCACGGATGACGCGCAGTTCGTCGCTGTCTAGCTCCACCATCGCCTGCACACCGGCGGCCAG-
ACCCATTGGCCGTCGCACTCGTA-
AGCAGGTAATCCTCGTCGACGGACTCGGTAACCACCGCCGCCAGCTCCGCTGCCAGGTCGGCGGGGTTGACACC
GGCGGGCATCGGGATGGACGACGACGCGGTGCTGACGGCGCCTGTC
::::::::::::::::X007T7.seq::::::::::::::::
AGCGGTTTCCCA-GCGGGATGTGCTGTGAGCGCCGCACCACCAGCGCCGACGCTAAGGATGGAACGCACGGCAT
CTTCTGACGCGTAACCGCGTTGTGATCGCGAGCTGAGGAGACGGTATGGGGGAGGGTTCTCGGAGGCCATCTGG
GATGTTGATGTCTGTCGATCTTGAGCCGGTGCAACTCGTCGGCCCGGACGGTACGCCGACGGCCGAACGCCGCT
ACCACCGTGACCTTCCTGAGGAAACGCTGCGTTGGCTCTACGAGATGATGGTGGTCACCCGCGAGCTGGATACC
GAATTCGTCAATCTGCACG
Clone X0008
::::::::::::::::X0008SP6.seq::::::::::::::::
CAAGCTTCCACAGGTAGGGATCGAGGAACAGCGCGTTGAACTGATAGGTGCGGCCCGGCTCGAGCAGGCCGGC
CATTTGTTCGATGCGGTTACCGAAAATCTCTTCGGTGACCTGCCCGCCGCCGGCCAGCTCGGCCCAGTGCCCGG
CGTTGGCCGCCGCGGCAACGATCTTGGCGTCCACGGTGGTCGGGGTCATGCCCGCGAGCAGGATCGGCGAGCG
GCCGGTCAGCCGGGTGAACTTCGTCGAAAGCTTGACCCTGCCGTCGGGGAGGCGAACCACGGTCGGTGCGTAN
CTCCACCAAGCCCGGGCAACCTCGGGGGTGGCGCC
::::::::::::::::X0008T7.seq::::::::::::::::
TGGACCTCATGACAACGCGGCGGCGATTACCCCCGCTACCGCCAGCAGCATGACGGCGGTAGCGAACACCGCC
GGATGCAGCGCAGGTGCGTCGATGTGCTCACGGAATCGCCCCGGCACCGCGATCTCGAGGATCACCAGTGCCAC
CCCCTGCAGCGCGACACCGACGATTCCGTACACCGCCACGCCGATCAGGCCCTGGGCCAGCTGGCGTATATGGC
GGCGATGGTGACGATGGCCAGCGCCACATACATTGTGGCGGCCAGAACCACGGCGTTGGGGCGGCGGTCGATG
AACACTAGGCGACGCAGATCGCCCGGGGTCAACAGGTTGACCATCAGAAAGCCTGCGA
Clone X0009
::::::::::::::::X0009SP6.seq::::::::::::::::
TTTGGTGCGGCCGGCAATCAACTTC-
GCTC-CAGCGGTTTCCCAGGCGGGATGTGCTGTGAGCGCCGCACCACCAGCGCCGACGCTAAGGATGGAACGCA
CGGCATCTTCTGACGCGTAACCGCGTTGTGATCGCGAGCTGAGGAGACGGTATGGGGGAGGGTTCTCGGAGGC
CATCTGGGATGTTGATGTCTGTCGATCTTGAGCCGGTGCAACTCGTCGGCCCGGACGGTACGCCGACGGCCGAA
CGCCGCTACCACCGTGACCTTCCTGAGGAAACGCTGCGTTGGCTCTACGATATGATGGTGGTCACCCG
::::::::::::::::X0009T7.seq::::::::::::::::
CGCCCAGGGCCGCTCCCGGGCGACCCGACCATTGCTGTCGCCGCGTAACGCCATCACGGATGACGCGCAGTTCG
TCGCTGTCTAGCTCCACCATCGCCTGCACACCGGCGGCCAGGACCCATTGGCCGTCGCACTCGTAGAGCAGGTA
ATCCTCGTCGACGGACTCGGTAACCACCGCCGCCAGCTCCGCTGCCAGGTCGGCGGGGTTGACACCGGCGGGC
ATCGGGATGGACGACGACGCGGTGCTGACGGCGCCTGTCGCGACGCTGAGCTCGGACACAGCTAGTAAATGTA
GCCTAACCTACTTAATGGGTCGCAGCCCCCCGGGGTCGTCGCATGTCCAACGTTGCTCGACTGGAAGAAAATGC
TCGTCGGGGAGCAAATGGCACC
Clone X0010
::::::::::::::::X0010SP6.seq::::::::::::::::
AATACTCAATCTTGATCGGTTTCCAGCAACAGCCGATCGACGGCTTCGCCCAGGGCCGCTCCCGGGCGACCCGA
CCATTGCTGTCGCCGCGTAACGCCATCACGGATGACGCGCAGTTCGTCGCTGTCTAGCTCCACCATCGCCTGCA
CACCGGCGGCCAGGACCCATTGGCCGTCGCACTCGTAGAGCAGGTAATCCTCGTCGACGGACTCGGTAACCACC
GCCGCCAGCTCCGCTGCCAGGTCGGCGGGGTTGACACCGGCGGGCATCGGGATGGACGACGACGCGGTGCTGA
CGGCGCCTGTCGCGACTCTGAGCTCGG
::::::::::::::::X0010T7.seq::::::::::::::::
GGATGTGCTGTGAGCGCCGCACCACCAGCGCCGACGCTAAGGATGGAACGCACGGCATCTTCTGACGCGTAACC
GCGTTGTGATCGCGAGCTGAGGAGACGGTATGGGGGAGGGTTCTCGGAGGCCATCTGGGATGTTGATGTCTGTC
GATCTTGAGCCGGTGCAACGCGTCGGCCCGGACGGTACGCCGACGGCCGAACGCCGCTACCACCGTGACCTTCC
TGAGGAAACGCTGCGTTGGCTCTACGAGATGATGGTGGTCACCCGCGAGCTGGATACCGAATTCGTCAATCTGC
AGCGCCAGGGGGAAGCTGGCGTTGTACACGCCCTGTCGCGGGCAGGAAGCCGCGCAGGTGGGTGCGGCGGCTT
GCCTACGCAAAACCGACTGGTTGTTCCCC
Clone X0012
::::::::::::::::X0012SP6.seq::::::::::::::::
ATCACGACAACAGCGACGGTGTGTCGGATCAGCGGCCCCCGTTGCCGGGCAATGTTGAGGCGTTTCTGCGTCTG
GTTGAGGCCGGCTGGGAC-
CCGAGGTGGCTCGTCGGCCACATGGGCAGCACACCACCGTGGTGATGCATCTAGACGTGCAGGACCGTGCCGCT
GGCCTGCA
::::::::::::::::X0012T7.seq::::::::::::::::
GCGGCTACGTGCCATCGAGACACTGGCGCAGGCTATCGCACCCGTTATCGGCTGCGAGCAAATCGCGGTATGCG
TTCTTGAGCATGAGTCGGCGACCGTCGTCATGGTCGACACCCACGACGGAAAGACGCAGATCGCCGTCAAGCAT
GTGTGCCGCGGATTATCAGGACTGACCTCCTGGCTGACCGGCATGTTTGGTCGCGATGCCTG
Clone X00013
::::::::::::::::X0013T7.seq::::::::::::::::
TACAAGCGGCACCTCGCCGGTGAACTGACCGTTCGCACGCTGCGCACCGCCGCCGGGCGCGTGCTCGGCGCGC
CGGCGGCCCCCGAGGCCTGAGAGGGGAACCAACCATGCAGGTGAACATGACGGTAAACGGCGAGCCCGTCACC
GCCGAGGTCGAACCCCGGATGCTGCTGGTCCATTTTCTCCGTGATCAGCTGCGGCTCACCGGAACTCACTGGGG
CTGTGATACCAGCAACTGCGGGACATGCGTGGTGGAGGTCGACGGCGTGCCGGTGAAATCCTGCACGATGCTCG
CCGTGATGGCCTCCGGGC
Clone X0014
::::::::::::::::X0014T7.seq::::::::::::::::
AGCGGCTGGTTACGACTCCCTGTTTGTGATGGACCACTTCTACCAACTGCCCATGTTGGGGACGCCCG-
CC-TCCGATGCTGGAAGCCTACACTGCCCTTGGTGCGCTGGCC-C-GCGACCGAGCGGCTGCAACTGGGCGC-
TTGGTGACC-GCAATACCTACCGCACCCC-ACCCTGCTGG-CAAA-
ATCATCACCACGCTCGACTTGGTTAGCGCCGGTCGA-CGATCCTCGGCATTGGAACCGGTTGGTTT-
Clone X0015
::::::::::::::::X0015SP6.seq::::::::::::::::
ACGCGCGCCGATCATATCTGCTATGGATGTACAATTCAGCTCTTGCTGTTATACCAGTATATGGTGTACTATTTG
ATCTATGCTGACGTGTGAGATGCGGGAATCGGCCCTGGCTCGACTCGGCCGGGCTCTGGCTGATCCGACGCGGT
GCCGGATTCTGGTGGCGTTGCTGGATGGCGTTTGCTATCCCGGCCAGCTAGCTGCGCACCTCGGGTTGACCCGA
TCGAATGTGTCCAACCATCTGTCGTGTTTGCGGGGCTGCGGGCTGGTA-
TCCCAACCTATGAGGGCCGGCAGGTTCGGTAT
::::::::::::::::X0015T7.seq::::::::::::::::
CCGCGCTGCTGCTGACGTCGGTCGAACGTGCGACACGTCTGCGAATACCGGCCGAACGCTGGGTTTATCCACAG
GCTGGCACCGACGCCCACGACACACCGGCCGTCGCCGACCGCCACCGACTGCATCGGTCGACGGCCATTCGGAT
CGCCGGTGCCCGGGCGCTGGAACTGGCTGGGCTGGGGCTCGATGACATCGAATACGTCGACCTGTATTCGTGCT
TTCCCTCCGCTGTCCAAGTCGCCGCAATCGAACTCGGCCTGGACACCGACGATCCTGCCCGCCCGCTGACCGTC
ACCGGGGGCCTGACCTTCGCCGGCGGGCCGTGGAGCAATTACGTCACGCACTCCAT
Clone X0016
::::::::::::::::X0016SP6.seq::::::::::::::::
CAGGCGTGCAATGACCTGCACTGCGCCGGA-A-TCCCTAACCCACTAAACCGGGGCCGCTCACAAGCCGTGCAGC
TCGGTCAGCGTCAGGTGCGCGACCAGGAA-
TAAATGAGCAGACCCGTGCCGTCAACGATGGTGGCGATCATCGGCCCCGAAACGATGGCCGGGTC-
ATGCGCAACTTCTTCAGCAGCGGCGGAAGGACGGCA-CCACCAGCGAC-ACCACACCACGAT
::::::::::::::::X0016T7.seq::::::::::::::::
GCGAA-CACTTCGTCAACTTCCAGGGCTGCCCGCACCAAGTATTTCGACGAGTATTTCCGTCGGGCCGCCGCCGC
CGGCGCGCGGCAGGTGGTCATCCTGGCGGCGGGGCTGGACTCGCGCGCGTACCGGCTGCCTTGGCCCGACGGG
ACCACGGTTTTTGAGCTGGACCGCCCGCAGGTCCTTGATTTCAAGCGCGAGGTGCTCGCCAGCCACGGTGCCCA
ACCGCGCGCCCTGCGCCGCGAGATCGCCGTCGACCTGCGTGACGATTGGCCACAAGCCTTGCGGGACAGTGGTT
TCGATGCGGCTGCACCGTCGGCATGGATTGCCGAAGGGCT
Clone X0017
::::::::::::::::X0017SP6.seq::::::::::::::::
TTGGGC-TTGCCC-CAAATA-GGCCCCAATCAAAAGCCGAGCAGGTGGAACCTA-CGATTCGCCTC-TCGT-TGTGC
ACCCGAGCCATCGCACGCGCGGGGAATTCCCGGAT-TC-CCGTATTCTCCGGCGGCCGGGCTAACCCATCCCA-GCC
GAACGGTTGGCTC-TGCCGTGGGTCCCGTGTTGGCCGATCGGGGCGTCACCGGGGTGCTCGGGTGCGG-
TGACCATGGC-AACTGCCCC-ATGGGCCGACCCTGGTGCAGATAAACCTG
::::::::::::::::X0017T7.seq::::::::::::::::
TGGTGGAGGTCCCCACCAA-ACCCGGCCGTAACTCTGCTCACGGAAATGCGG-CAGGCCGCGCGTAGCACGTGGT
ATCCGCCATAAAGGTGCACCTTAAGCACGGCGTCCCAATTCTCGAACGACATCTTGTGGAAGGTGCCGTCGCGC
AAGATCCCGGCGTTGCTCACCACACCGTGCACGGCGCCGAATTCGTCAAGCGCGGTCTTGATGATGTTCGCTGC
GCCGTCCTCGGTGGCGACGCTGTCGGTA-TTGGCGACCGCCCGGCCCCCCTTGTCGCGAAATCTCGGCGACGAC
CTCATCGGCCATCGCCGAACCGGGCGCCCG
Clone X0018
::::::::::::::::X0018SP6.seq::::::::::::::::
GCCGGCCAAACTGGCCGGCGGGGTTGCTGTC-TCAAGGTGGGTTCCGCCACCAA-ACC-
CACTCAAGGATCGCAAGGAAAGC-
TCAAGGATGCGGTCGCGGCCGCCAAGGCCGCGGTCAAGGAGGGCATCGTCCCTGGTGGGGGA-CCTCCCTCATC
CACCAGGCCCGCAAGGCGCTGACCGAACTGC-TGCGTC-C-GACCGGTGACAA-GTCCTCGGTGTCCACGTGT-CTC
CGAAGCCCTTGCCGCTCCGTTGTTCTGGATC-CC-CCAAC-CTGGCTTGGACGGCTC-
GTGGTGGTCAACAAGGTCAGCGAGCTACCCGCCGGGCATGGGCTGAACGTGA
Clone X0018
::::::::::::::::X0018T7.seq::::::::::::::::
CGACCT-
AATTGTCCTGTAATGCCCAGCTCACCAA-GCATGGCTGGTGGCCGGGGCGGTGAAGCCGGCGTCTGCGGCACCG
TCCAACTC-ATGTGGAT-GCCGGAATGGGGATGTCCGG-ACGGCGAATCCGTA-
TTCGCTTGTCCCGTGAGGCCCAGGTGGATGGGGGGAAGGATC-TGGTGTCCGGGATGAT-
ATGGGGCCGATGCCGCCGGTTGAAGTCCACTGGATCGGGAATTCGGGAATCGTGAT-
CCGACGTTCAGGCCGAAC
Clone X0019
::::::::::::::::X0019SP6.seq::::::::::::::::
CTAACGGAATGAAAGCCCTGGTGGCCGT-TCGGCGGTGGCCGTCGTCGCACTGCTCGGTGTATCTTCCGCCCAAG
CTGATCCCGAGGCGGATCCCGGCGCAGGTGAGGCCAACTATGGTGGCCCCCCAAGTTCCCCACGTCTTGTCGAT
CACACCGAATGGG-CGCA-
TGGGGAATTCTGCCCAGCCTCCGGGTCTACCCGTCCCAAGTTGGGCGTACA-CCTCCCGCCGCCTCGGGATGGCC
GCTGCCGACCCGGCCTGGGCC-AGGTTCTCGCGCTGTCACCGGAAGCCGACACTGCCGGC
::::::::::::::::X0019T7.seq::::::::::::::::
CCGCGGGACAC-CCTC-ATGCTGCCGCCATGGACGCGGTCGAACGCAAGCAGCTGATCGAGCTACAACGCCGCG
CGGAACGCTTCCGCCGCGGGCGTGACCGCATCCCGTTGACCGGGCGGATCGCGGTGATCGTCGATGACGGCATC
GCCACCGGAGCGACGGCCAAGGCGGCGTGCCAGGTCGCCCGGGCGCACGGTGCGGACAAGGTGGTGCTGGCG
GTCCCGATCGGCCCA-ACGACATCGTGGCGAAGATTCGCCGGGTACGCCGATGATGTGGTGTGTTTGGCGACGC
CGGCGTTGT
Clone X0020
::::::::::::::::X0020T7.seq::::::::::::::::
CTCTGGGACCGGCCACGGTGCC-CCGGCGTTCCCGGACGTGCTGCGCCAGGTGTCCGGCGGCCGCGTGCATGGT
GTTCCCGGATCGGCCGCTGGCCAGAGCCCACCGGTGAATCTGGCGCCTGGCCGACCACCGTGCGCCGTAGGCTT
GCGATCGTGCAGCGCTGGCGTGGCCAGGACGAGATCCCGACGGATTGGGGCAGATGCGTGCTCACCATCGGGG
TATTTGACGGCGTGCACCGCGGGCACGCCGAACTGATCGCGCACGCGGTCAAAGGCGGC
Clone X0021
::::::::::::::::X0021SP6.seq::::::::::::::::
AATACTCAAGCTTTCGTCAGTTCATTGCGCCAGCAGACCAACAA-AGCATCGGGACATACGGA-TCAACTACCCG
GCCAACGGTGATTTCTTGGCCGCCGCTGACGGCGCGAACGACGCCAGCGACCAC-TTCAGCAAATGGCCA-CGCG
TGCCGGGCCACGAGGTTGGTGCTCGGCGGCTACTCCCAGGGTGCGGCCGTGATC-ACATC-TCACCGCCGCACCA
CTGCCCGGCCTCGGGTTCACGCAGCCGTTGCCGCCCGCAGCGGAC-
ATCACATCGCCGCGATCGCCCTGTTCGGGAATCCCTC-GGCCGCGCTGGCGGGCTGATTAAC
::::::::::::::::X0021T7.seq::::::::::::::::
TGCCGCGGATTTGGCTGGCTGCCCAATATTCAGAATCGGGCCTTTCTTTTTGCGCGACAATAAGGTCACAGTAAA
CCCTCGTTTTGTGAGATGCGGGGCGGGCCGGGCGAA-TCGACCTCGAGTGAATGGATCTCGAGTGAATGGACAG
GGCATCGCCTACGAGTCGCATCCCCATCCAACAGACCGGTGCTCTTGCATCGGACCCTGAAGGTCCCGCACGGA
GGGTGTGGTTGCCGGCGCGGGGTCACGGTGCGGTAGCGACGTAGTGTTTGAACGAATTTCTTGATGCTCCAACC
TGTTTGGTGTTCAATCCAGTTCT
Clone X0175
.....X0175SP6.....
AA-CTTGCGCGCTCGGCCGGGTC-AGCATCCAGCTGCTCGGCAAGGAGGCCAGCTAC-C-TCGCTGCGTATGCCCA
GCGGTGAGATCCGCCGGGTC-ACGTCCGCTGCCGCGCGACCGTCGGCGAAGTGGGCAATGCCGAGCAGGCAAAC
ATCAACTGGGGCAAGGCCGGTCGGATGCGGTGGAAGGGCAAGCGCCCGTCGGTCCGGGGCGTGGTGAT-AACC
CGGTC-ACCACCCGCACGGCGGTGGTGAGGGTAAAACCTCCGGCGGCCGTCACCCGGTTAGCCCGTGGGGCAA
.....X0175T7.....
A-TCGAAAGTGACCATCTCTACCTTGAGTGCCATACCGCCCGACCCTATGCCTCGGATAGCTCGGCGGAAAGAAA
CGCTTGCAGTGCCGCCGAATAGGCGGCTACGTCGTGAGCGCCCATCAACTCTCGCGCGGAGTGCATCGCCAGCT
GGGCGGCGCCGACGTCGACCGTGGGGATTCCGGTGCGCGCCGCGGCCAACGGCCCGATCGTCGACCCGCACGG
CAGATCGGCGCGATGTTCGTAACGCTGCATAGGCACTCCCGCGCGCTGGCAGGCCAGTGCGAACGCCGCCGCG
GTGCGTCCG
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