Concentrated water-dispersible vitamin compositions

Abstract

A water-dispersible vitamin composition containing Vitamins A, D3 or E, or a precursor thereof, an alkyl lactate and an emulsifier and is substantially free of water.

Description

The present invention relates to new water-dispersible compositions to provide one or more of vitamins E, A and D₃ to an animal.

The present invention provides a water-dispersible liquid vitamin composition comprising:

a) from 40% to 90% by weight of a vitamin component selected from the group consisting of:

• • (i) one or more precursors of Vitamin A;
  • (ii) one or more precursors of Vitamin E;
  • (iii) a mixture of one or more precursors of Vitamin A and one or more precursors of Vitamin E;
  • (iv) a mixture of one or more precursors of Vitamin A and Vitamin D3;
  • (v) a mixture of one or more precursors of Vitamin E and Vitamin D3; and
  • (vi) a mixture of one or more precursors of Vitamin A, one or more precursors of Vitamin E, and Vitamin D3;

b) from 2% to 10% of a C1 to C6 alkyl lactate;

c) from 0% to 10% of a C2 to C6 mono-hydroxy alcohol;

d) from 5% to 50% of a one or more veterinarily acceptable emulsifiers; and

e) from 1% to 12% of an oil;

wherein the composition is substantially free of water.

Component a) of the composition may be from 45% to 85% by weight.

It should be understood that in the present specification and claims by the term “%” is meant percent by weight unless expressly noted otherwise.

In certain embodiments of the present invention, the liquid vitamin composition consists essentially of the ingredients listed in the foregoing description of the composition.

The vitamins are suitably provided as the oily derivative of the vitamin, such as the lower alkyl esters of the vitamin as a solution or suspension in an oil (e.g., a veterinarily acceptable oil). By the term lower alkyl is generally meant C1-C6 alkyl which is optionally substituted by one or more halogens. For example Vitamin A may be provided as retinyl propionate. Vitamin E may be provided as, e.g, (DL) alpha tocopheryl acetate. Suppliers of the vitamins or precursors thereof include Hoffman LaRoche Corporation, and Aventis Animal Nutrition. Such products are generally known to those skilled in the art.

The oil is, e.g., a fatty acid oil, such as a vegetable oil, as needed to provide-the compositions. Such oils are acceptable as food additives and known to the person skilled in the art. Such oils include soybean oil, corn oil, canola oil, peanut oil and the like. In the case of vitamin E, or its precursor dl-alpha tocopheryl acetate, the oil may be composed of or include a manufacturing by-product.

In another aspect of the invention, the composition may further comprise from 0 to 5% of a stabilizer. The stabilizer enhances vitamin stability and keeps the composition as a flowable liquid for an extended period of time, generally from 1 to 6 months. The stabilizer may also function as an anti-gelling agent and/or as an antifreeze. Suitable stabilizers include propylene glycol, sorbitol and glycerine. The preferred agent is propylene glycol.

In another aspect of the invention, the ratio of the alkyl lactate to the alcohol is from 1:1 to 3:1, preferably from 1.5:1 to 2.8:1.

Examples of mono-hydroxy alcohols include ethanol, n-propanol or isopropanol. The mono-hydroxy alcohol is preferably a normal alcohol. Propanol is highly preferred. In one embodiment of the present invention, the amount of mono-hydroxy alcohol may be from about 2% to 5%, or from about 3% to 5%.

Alkyl lactates that may be used according to the invention include methyl lactate, ethyl lactate, n-propyl lactate, iso-propyl lactate, n-butyl lactate, iso-butyl lactate, sec-butyl lactate, tert-butyl lactate, n-pentyl lactate, n-hexyl lactate and other isomeric forms thereof. The alcohol portion of the ester may be optionally substituted by one or more halogens. Ethyl lactate and butyl lactate are examples of preferred alkyl lactates that are used in the invention. All enantiomeric and diasteromeric forms of lactate esters are embraced by the present invention. Lactate esters of L(+) lactic acid are generally preferred.

By the term substantially free of water may be meant less than 1%, or less than 0.5% or less than 0.1% by weight water in the composition.

The emulsifiers according to the present invention may be of any type of emulsifiers, and are preferably non-ionic surfactants. Examples of non-ionic surfactants include polyethylene glycol esters and ethoxylated sorbitan fatty acid esters. Examples of these groups of surfactants include Polysorbate 80, Polysorbate 80K, PEG 400, Alkamuls® PSMO-20, Alkamuls® 400-MO, T-MAZ 80K, MAPEG® 400 Mo and the like and are generally known to those skilled in the art.

The compositions of the present invention may have viscosities that are from 1000 cP to 10000 cP at 0° C. and from 50 cP to 2000 cP at 10° C. It is understood that viscosities are measured by the Brookfield method known to those of skill in the art and specifically described in example 7 of this application.

Another aspect of the present invention is that the composition may be quickly dispersed in water. That is the composition adequately disperses into water within 2 minutes, preferably within 20 seconds, when added at a ratio of composition to water of from 1 g/kg to 100 g/kg, preferably from 3 to 10 g/kg. By the term “adequately disperses” is meant that the composition disperses into water and forms a finely dispersed emulsion (e.g., under the conditions specified in Example 6).

The composition may also comprise a fungicide. Any suitable fungicide acceptable in veterinary medicine may be used and in particular potassium sorbate is preferred. When a fungicide is used, it is present in the composition in trace amounts, for example, from about 0.05% to 0.3% by weight of the composition.

The concentration of each vitamin in the compositions may be varied to satisfy the specific requirements of the product desired. Generally, Vitamin A is present in an amount of from 800,000 to 1,200,000 IU/g, preferably from 800,000 to 1,000,000 IU/g. Vitamin D3 is generally present in an amount of from 50,000 to 1,200,000 IU/g, preferably from 100,000 to 1,000,000 IU/g. Vitamin E is generally present in an amount of from 100 to 1000 IU/g, preferably from 200 to 750 IU/g.

The specific products set forth in Table 1 are contemplated by the invention. Stock solutions or suspensions of vitamins and vitamin precursors are generally supplied at specific International potency units per/gram (IU/g). For example, the precursor to Vitamin A used in the formulations of Table 1 is retinyl propionate which is used as a 79% by weight solution of retinyl propionate dissolved in canola oil and stabilized with 1% by weight ethoxyquin. This means that the retinyl propionate/canola oil solution contains s about 2,200,000 IU/g of Vitamin A (pure all trans retinyl propionate has a theoretical potency of 2,780,000 IU/g). The Vitamin D3 concentrate used in Table 1 is an oily concentrate prepared from Vitamin D3 resin and contains about 10% by weight Vitamin D3 in vegetable oil. The Vitamin D3/vegetable oil concentrate is stabilized with BHA or BHT and has about 4,000,000 IU/g of Vitamin D3 (pure cholecalciferol, also known as Vitamin D3, has a theoretical potency of 40,000,000 IU/g). The Vitamin E precursor used in Table 1 is 94.5% by weight pure dl-alpha tocopheryl acetate in oil (945 IU/g).

TABLE 1
				% weight of
			Vitamin	Vitamin or
Product Name	Vitamin	Precursor	Potency (IU/g)	precursor	% weight oil
A1000	A	Retinyl propionate	1,000,000	38.7	10.3
AD3 1000/200	A	Retinyl propionate	1,000,000	38.7	10.3
	D3	None	200,000	0.52	4.68
AD3 1000/100	A	Retinyl propionate	1,000,000	38.7	10.3
	D3	None	100,000	2.34	0.26
AD3E 800/200/200	A	Retinyl propionate	800,000	31.0	8.2
	D3	None	200,000	0.52	4.68
	E	dl alpha tocopheryl	200	20.6	1.2
		acetate
E60	E	dl alpha tocopheryl	600	61.8	3.6
		acetate
E75	E	dl alpha tocopheryl	750	77.3	4.5
		acetate
E80	E	dl alpha tocopheryl	800	82.4	4.8
		acetate

EXAMPLE 1

A composition containing the ingredients in Table 2 was prepared by sequentially adding, under agitation, 94.5% by weight pure dl-alpha tocopheryl acetate in oil, emulsifier (PEG 400), ethyl lactate and propanol and mixing until homogeneous (generally two hours).

	TABLE 2
	Ingredient	Kilograms	PerCent
	94.5% pure dl-alpha tocopheryl	817.5	81.75
	acetate (in oil)
	PEG 400	82.5	8.25
	Ethyl Lactate	70	7.0
	Propanol	30	3.0
	Total	1000	100

EXAMPLE 2

A composition containing the ingredients in Table 3 was prepared by the procedure of Example 1 where products were added sequentially under agitation and mixed until homogeneous.

	TABLE 3
	Ingredient	Kilograms	PerCent
	Retinyl Propionate 79% in canola oil	489.5	48.95
	Polysorbate 80	61.0	6.10
	PEG 400 MO	339.5	33.95
	Ethoxyquin	30.0	3.00
	Ethyl Lactate	50.0	5.00
	Propanol	30.0	3.00
	Total	1000.0	100

EXAMPLE 3

A composition containing the ingredients in Table 4 was. prepared by the procedure of Example 1 where products were added sequentially under agitation and mixed until homogeneous.

	TABLE 4
	Ingredient	Kilograms	PerCent
	Retinyl Propionate 79% in canola oil	489.5	48.95
	Vitamin D3 10% in oil	26.3	2.63
	Polysorbate 80	30.0	3.00
	PEG 400 MO	274.2	27.42
	Ethyl Lactate	50.0	5.00
	BHT	30.0	3.00
	Propanol	100.0	10.00
	Total	1000.0	100

EXAMPLE 4

A composition containing the ingredients in Table 5 was prepared by the procedure of Example 1 where products were added sequentially under agitation and mixed until homogeneous.

	TABLE 5
	Ingredient	Kilograms	PerCent
	Vitamin D3 10% in oil	52.5	5.25
	94.5% pure dl-alpha tocopheryl	218.0	21.8
	acetate (in oil)
	Retinyl Propionate 79% in canola oil	391.6	39.16
	Polysorbate 80	37.9	3.79
	PEG 400 MO	188.0	18.80
	Ethoxyquin	30.0	3.00
	Ethyl Lactate	50.0	5.00
	Propanol	32.0	3.20
	Total	1000.0	100

The viscosity of the composition was 1300 cP at 0° C. and 500 cP at 100° C. The composition adequately dispersed into water at 100 g/kg water at 20 degrees C.

EXAMPLE 5

A composition containing the ingredients in Table 6 was prepared by the procedure of Example 1 where products are added sequentially under agitation and mixed until homogeneous

	TABLE 6
	Ingredient	Kilograms	PerCent
	Vitamin D3	52.5	5.25
	Retinyl Propionate	489.5	48.95
	Polysorbate 80	70.0	7.00
	PEG 400 MO	276.0	27.60
	Ethoxyquin	30.0	3.00
	Ethyl Lactate	50.0	5.00
	Propanol	32.0	3.20
	Total	1000.0	100

The viscosity of the composition was 800 cP at 0° C. and 350 cP at 10° C. The composition adequately dispersed into water at 100 g/kg water at 20 degrees C.

EXAMPLE 6

About 5 drops (approx 0.15 g) of composition is added to 50 ml of water (room temperature which is from about 15° C. to about 25° C.) in a 100 ml flat-bottomed beaker and stirred with rod manually for 20 seconds. The resulting emulsion is examined and rated according to the following scale.

1. No emulsion or dispersion; composition is separated from water
2. Partial emulsion/dispersion but large agglomerations of test
   product are observed
3. Almost complete emulsion/dispersion; small
   agglomerations are visible
4. Complete emulsion/dispersion; homogeneous liquid without
   any visible agglomerations

The compositions of Examples 1, 2, 3, 4 and 5 adequately dispersed into water at 100 g/kg water at 20° C.

EXAMPLE 7

Viscosity is measured with a Brookfield DV-II+Viscometer (Brookfield Engineering Labs, Middleboro, Mass.) using RV spindle #3. The composition to be tested was placed in a 200 ml flat-bottomed sample jar and chilled at −20° C. for several hours. The viscometer functions in combination with a microcomputer supplied by Brookfield and uses a timed-stop program whereby a viscosity reading was taken every 30 seconds. Viscosity, torque and temperature are measured until the sample warmed to about from 10° C. to 15° C.

The following viscosities were recorded:

Example	Temp. A	Viscosity A	Temp B	Viscosity B
1	0° C.	6000 cP	10° C.	1200 cP
2	0° C.	800 cP	10° C.	400 cP
3	0° C.	250 cP	10° C.	200 cP
4	0° C.	1300 cP	10° C.	500 cP
5	0° C.	800 cP	10° C.	350 cP

It should be understood that the preceeding is merely a detailed description of the embodiments of the invention and that numerous changes to the disclosed embodiments can be made in accordance with the disclosure herein without departing from the spirit or scope of the invention. The preceding description, therefore, is not meant to limit the scope of the invention. Rather, the scope of the invention is to be determined only by the appended claims and their equivalents.

Claims

1. A water-dispersible liquid vitamin composition comprising:

a) from 40% to 90% by weight of the composition of a vitamin component dissolved in from 1% to 12% by weight of the composition of an oil the vitamin component being selected from the group consisting of: (i) one or more precursors of Vitamin A; (ii) one or more precursors of Vitamin E; (iii) a mixture of one or more precursors of Vitamin A and one or more precursors of Vitamin E; (iv) a mixture of one or more precursors of Vitamin A and Vitamin D3; (v) a mixture of one or more precursors of Vitamin E and Vitamin D3; and (vi) a mire of one or more precursors of Vitamin A, one or more precursors of Vitamin E, and Vitamin D3,

b) from 2% to 10% of a C1 to C6 alkyl lactate;

c) from 0% to 10% of a C2 to C6 mono-hydroxy alcohol;

d) from 5% to 50% of a one or more veterinarily acceptable emulsifiers; and

wherein the composition is substantially free of water.

2. The composition of claim 1, wherein said composition further comprises from 0% to 5% of a stabilizer.

3. The composition of claim 1, wherein said composition comprises said alkyl lactate and said mono-hydroxy alcohol and the ratio of the alkyl lactate to the mono-hydroxy alcohol is from 1:1 to 3:1.

4. The composition of claim 3, wherein the ratio of b) to c) is from 1.5:1 to 2.8:1.

5. The composition of claim 1, wherein the C2-C6 mono-hydroxy alcohol is present in an amount from 2% to 5%.

6. The composition of claim 1, wherein the emulsifier is a non-ionic surfactant.

7. The composition of claim 1, wherein the viscosity of the composition is from 1000 cP to 10000 cP at 0 degrees C.

8. The composition of claim 1, wherein said composition adequately disperses into water within 2 minutes when added at a ratio of composition to water of from 1 g/kg to 100 g/kg.

9. The composition of claim 1, wherein said composition adequately disperses into water within 20 seconds when added at a ratio of composition to water of from 1 g/kg to 100 g/kg.

10. The composition of claim 1, wherein the C1 to C6 alkyl lactate is ethyl lactate.

11. The composition of claim 1, wherein the C2 to C6 mono-hydroxy alcohol is propanol.

12. A food additive comprising the composition of claim 1.

13. A water-dispersible liquid vitamin composition comprising.

a) from 40% to 90% by weight of the composition of a vitamin component dissolved in from 1% to 12% by weight of the composition of an oil, the vitamin component being selected from the group consisting of. (i) a precursor of vitamin A; (ii) a precursor of vitamin E; (iii) a mixture of a precursor of vitamin A and a precursor of vitamin E; (iv) a mixture of a precursor of vitamin A and a precursor of vitamin D3; (v) a mixture of a precursor of vitamin E and a precursor of vitamin D3; (vi) a mixture of a precursor of vitamin A, a precursor of vitamin E and a precursor of vitamin D3;

b) from 2% to 10% of C1 to C6 alkyl lactate;

c) from 0% to 10% of a C2 to C6 mono-hydroxy alcohol;

d) from 5% to 50% of one or more veterinarily acceptable emulsifiers;

wherein the composition is substantially free of water.

14. A water-dispersible liquid vitamin composition comprising.

a) from 40% to 90% by weight of the composition of a vitamin component dissolved in from 1% to 12% by weight of the composition of an oil, the vitamin component being selected from the group consisting of: (i) retinyl propionate; (ii) dl alpha tocopheryl acetate; (iii) a mixture of retinyl propionate and dl alpha tocopheryl acetate; (iv) a mixture of retinyl propionate and vitamin D3; (v) a mixture of dl alpha tocopheryl acetate and vitamin D3; and (vi) a mixture of retinyl propionate, dl alpha tocopheryl acetate and Vitamin D3;

b) from 2% to 10% of C1 to C6 alkyl lactate;

c) from 0% to 10% of a C2 to C6 mono-hydroxy alcohol;

d) from 5% to 50% of one or more veterinarily acceptable emulsifiers;

wherein the composition is substantially free of water.