Dispensing device and method for forming material
A dispensing device and method for forming a least partially solid or gel-like material from a liquid. At least one liquid issuing from an outlet is subjected to an electric field causing the liquid to form at least one electrically charged jet which, after formation, forms a fiber (F) or breaks up into fiber fragments (FF) or particles (D). The thus formed at least partially solid or gel-like material may be directly deposited, by virtue of the energy in the electrical field, onto a surface area, for example an area of skin enabling, for example, formation of a dressing for a wound or burn which is of high specific area and extremely absorbent. A biologically active ingredient such as a pharmaceutical ingredient or biological matter such as DNA may be incorporated into the fibers (F), fragments (FF) or particles (D). Fibrils, particles or microcapsules incorporating a biologically active ingredient may be supplied for oral or nasal administration to an animal such as a human being.
This invention relates to methods and devices for forming material. In one example, this invention relates to methods and devices for applying material to a surface, for example to an internal or external surface of an animal, for example for applying material to skin for use, for example, in the care or treatment of wounds or burns.
Various forms of aerosol devices for allowing material to be sprayed onto a surface such as the human skin are known, including aerosol devices for spraying wound care products onto wounds or burns. One such product is Savlon Dry (trade mark) which has been marketed in the UK by Zyma Healthcare and Ciba Geigy plc. Such products require the use of a gas propellant and in recent years the choice of gas propellants has become more limited because of the desire to avoid environmentally unfriendly compounds such as a chlorofluorocarbons or hydrocarbons. Also because small droplets and powder particles tend to be carried away from the target by the gas flow created when the propellant gas hits and is deflected by the target surface, such gas propelled sprays are generally designed to spray relatively large droplets or powder particles in order to achieve sufficient inertia to deposit the spray on its target surface. Such gas propelled products may run if sprayed too freely, especially where the spray produces large droplets. In addition, the packaging costs for such devices are high.
GB-A-1569707 describes a dispensing device for producing a spray or cloud of liquid droplets intended primarily for crop spraying. The process described in GB-A-1569707 produces liquid droplets by applying an electric field to a liquid emerging from an outlet in the vicinity of the surface so that the liquid becomes sufficiently charged that the net electric charge in the liquid as the liquid emerges into free space counteracts the surface tension forces of the liquid and the repulsive forces generated by the like electrical charges cause the liquid to be comminuted to produce a cone or jet which breaks into liquid droplets. The droplets produced by this device are charged close to their Rayleigh Limit and thus in use migrate quickly toward conductive surfaces of lower or zero potential. This technique of comminuting liquid is generally known as electrohydrodynamic comminution.
In one aspect, the present invention provides a method and/or a device for forming solid, partially solid or gel-like matter such as fibres, fibrils or fibre fragments or segments, droplets or particles by an electrohydrodynamic process. The thus formed matter may incorporate or have a core of a different material which may be for example a biologically active ingredient or material. The formed matter may be applied to a surface or area such as, for example, the surface of the skin or a wound or burn or to a cavity, for example a body cavity. The body cavity may be the respiratory system of an animal such as a human being, where the electrohydrodynamic process produces matter that does not block the respiratory system.
Where the resulting matter or material is to be applied or supplied to a cavity or concave surface, then desirably the matter is at least partially electrically discharged before application or supply.
In another aspect, the present invention provides a method or device for forming a mat or web by electrohydrodynamically forming electrically charged fibres and/or fibrils in the vicinity of a surface or substrate. The present invention also provides a mat or web formed using an electrohydrodynamic process.
In an aspect, the present invention provides a method or device for applying material to a surface by supplying to an electrohydrodynamic site located in the vicinity of the surface liquid which is electrohydrodynamically processed at the site in such a manner so as to form matter comprising at least partially solid or gel-like fibres, fibre fragments or fibrils or particles which are charged and are electrostatically attracted to the said surface enabling a mat or web of randomly distributed fibres and/or fibrils and/or particles to be formed on the surface. The location at which the matter is deposited on the surface can be at least partially controlled by effecting relative movement between the surface and the matter.
In another aspect, the present invention provides a method of applying material to an exposed surface of an animal, for example to the skin or to a wound or burn or area exposed by a surgical procedure, which comprises producing material comprising at least one of electrically charged fibres, fibre fragments or fibrils or droplets or particles in the vicinity of the said surface area by an electrohydrodynamic process, so that the material deposits on the said area.
In another aspect, the present invention provides a method of forming fibre fragments or fibrils by supplying liquid to an electrohydrodynamic site and deliberately perturbing the cone or jet issuing from the comminution site to cause the resulting fibre to break up into fragments. The break up of the fibre may be promoted by pulsing the voltage used for the electrohydrodynamic process. The length of the fibrils may be controlled by adjusting the frequency of the pulses.
In another aspect, the present invention provides a method of forming at least partly solid droplets or particles by supplying liquid to an electrohydrodynamic comminution site.
In an example, the present invention provides a method of depositing fibres on a surface, for example to form a dressing for a surface area of an animal for example an area of skin, a wound or burn or for other therapeutic or cosmetic reasons, which comprises supplying liquid comprising polylactic acid having a molecular weight in the region of 144000, dissolved 10% by mass in acetone at approximately 10 millilitres per hour to an electrohydrodynamic comminution site located at about 5 to 10 cm above the surface.
In another example, the present invention provides a method of depositing fibres on a surface, for example to form a dressing for a surface area of an animal for example an area of skin, a wound or burn or for other therapeutic or cosmetic reasons, which comprises subjecting liquid comprising a biocompatible polymer which may be bioresorbable or biodegradable polymer such as polylactic acid, polygylcolic acid, polyvinyl alcohol or polyhydroxybutyric acid to an electrohydrodynamic process in the vicinity of said area.
In an embodiment, the deposition process may be repeated one or more times to provide a number of layers of material comprising at least one of fibres, fibrils, droplets or particles on the surface. The polarity to which the material is charged may be reversed between deposition of different layers so as facilitate attraction between the layers.
The liquid used to produce the electrohydrodynamically formed matter may comprise a biologically active ingredient or component. Where the electrohydrodynamically formed material comprises fibrils, the fibrils may actually stick into the skin of soft tissue enabling delivery of the active component to a location beneath the outer layer of skin or soft tissue.
The liquid used may comprise a solution, suspension, microsuspension, emulsion, microemulsion, gel or even a melt which may contain an active component or components. Alternatively or additionally, the active component may be provided as a coating or a core of the fibre, fibril or particle. For example microcapsules, fibres or fibrils of a bioresorbable or biodegradable polymer may be formed which contain a biologically active ingredient. Material from the core of a fibre or fibril may be released from the ends of the fibre or fibril. Material from the core of a fibre, fibril or microcapsule may be released through the coating if the coating is permeable to the material contained within it or may be released as a result of the outer coating being breached, for example by chemical or enzymic attack which causes the outer coating to dissolve or degrade, by bioresorption or biodegradation of the coating, or as a result of temperature changes or application of pressure which causes the outer coating to rupture. The timing of the release may be controlled, for a given polymer, by controlling the thickness of the coating surrounding the core.
Possible biologically active components for topical application are pharmaceutical compounds such as analgesics, antiseptics, antibiotics, antifungals, antibacterials, antiparasitics, debridement agents such as proteolytic enzymes, biological products such as cells, and cytokines for stimulating cytokinetic activity to promote essential cell activities, for example, to stimulate dendritic growth, growth factors such as fibroblast growth factor (FGF), epithelial growth factor (EGF), transforming growth factor (TGF) and others that may be used to promote or otherwise control the sequence of events essential to natural tissue repair, DNA or other genetic material for gene therapy, cells, peptides or polypeptides, insulin, adjuvants, immune suppressants or stimulants, surface binding or surface recognising agents such as surface protein A, and surfactants. Where more than one layer of fibres, fibrils or droplets is deposited, then different active ingredients may be provided in different layers.
Fibres, fibre fragments or particles of biological material such as fibrin or collagen may be formed using a method embodying the invention. Also electret polymers may be used to act as nuclei or otherwise initiate interactive cellular and/or molecular events in tissue repair.
A number of electrohydrodynamic processing sites may be provided enabling different types of electrohydrodynamically formed matter to be deposited at the same time.
The deposited material may be used alone or in combination with a conventional bandage or dressing. As another possibility, where the material contains, for example, a therapeutic agent, the material may be deposited onto a conventional dressing to be applied to the skin.
In another aspect, the present invention provides a method or device for supplying comminuted material to the respiratory system of an animal, which comprises electrohydrodynamically comminuting liquid so as to produce a plurality of at least partially solid or gel-like fibrils or particles and supplying the fibrils or particles orally or nasally to the animal. The comminuted material is preferably at least partially electrically discharged before supply to the animal especially if it is to be delivered to the upper or lower reaches of the lungs rather than simply to the nasal or oral passages.
The fibrils or particles may comprise biologically active material, for example the fibrils or particles may comprise DNA encapsulated in or complexed with a lipid for transfecting cells or may, for example, contain or encapsulate matter such as peptides, polypeptides and other large biomolecules such as insulin or growth factor, and/or active pharmaceutical components for enabling delivery of the active component into the blood stream via the lung. This should provide a quicker route to the bloodstream than that provided by normal oral ingestion and avoids the need for injection of components which cannot be taken orally because of the gastric enzymes and acids present in the digestive system. Microcapsules or fibrils for oral ingestion of appropriate active components enabling slow release of those components may also be produced by electrohydrodynamic means by providing the active component as the core of the capsule or fibril.
Embodiments of the present invention will now be described, by way of example, with reference to the accompanying drawings, in which:
Referring now to the drawings,
In use of the apparatus, a surface area 7 such as an area of the skin of an animal, for example an area of skin of a human being, is positioned a few centimetres, for example from 5 to 10 cm, below the outlet 4 as shown schematically in
As described in the aforementioned GB-A-1569707 and an article entitled “Electrodynamic Crop Spraying” by R. A. Coffee published in Outlook on Agriculture Volume 10 No. 7 1981, liquid issuing from the outlet 4 is subject to an intense electrical field which establishes a standing wave along the surface of the liquid producing cusps or cones which emit jets of charged liquid.
The small perturbations which inevitably occur in the liquid jet cause the jet to become unstable and the net electrical charge in the liquid provides a repulsive force which acts against the surface tension forces in the liquid. This would normally be expected, as described in GB-A-1569707, to cause the liquid to break up into droplets which, because both they and the outlet 4 are similarly charged, are propelled away from the outlet 4 and each other so providing a spray or cloud of liquid droplets. The present inventors have, however, found that by selecting the liquid and controlling the conditions of the electrohydrodynamic process, the jet of liquid, rather than breaking up into liquid droplets, forms a solid or gel-like fibre or forms fibre fragments (fibrils) or non-liquid droplets or particles. In use of the apparatus shown in
Relative movement may be effected between the nozzle 4 and the surface 7, in this example the surface 7 may be moved, to enable coverage of a large area.
In order to form the solid or gel-like droplets shown in
To form the fibrils or fibre fragments shown in
In order to form the solid or gel-like fibre F shown in
The apparatus 1 shown in
The apparatus 1a shown in
The pump chamber 10 may comprise any suitable form of pump, which provides a continuous substantially constant flow rate, for example an electrically operable pump such as a piezoelectric, or diaphragm pump or an electrohydrodynamic pump as described in EP-A-0029301 or EP-A-0102713 or an electroosmotic pump as described in WO94/12285 or a mechanically-operable pump such as syringe pump operated or primed by a spring biassing arrangement operable by a user.
In use of the apparatus 1a shown in
Various different forms of outlet or nozzle 4 may be used in the apparatus shown in
The nozzle 4a shown in
The nozzle 4b shown in
The nozzle 4c shown in
The nozzle 4d shown in
Where, as discussed above, the liquid being used is sufficiently conductive to enable the voltage to be applied to the liquid rather than the nozzle then the nozzle may be formed of any suitable electrically insulative material which does not retain electrical charge for any significant length of time, for example glass or a semi-insulating plastic such as polyacetyl.
The nozzle shown in
In the case of the cylindrical nozzle shown in
The use of a liquid which is controlled to produce fibres is particularly advantageous for producing a wound or burn dressing because, as will be described below, deposition of the fibres onto the area being covered results in a network of crossing or interlinking fibres providing effectively an integral web or mat which has a high specific surface area and is thus highly absorbent to fluids, whilst being exceptionally light. Like a conventional dressing it enables good coverage over an area of skin so as, for example, to protect a wound but, unlike many conventional dressings, still enables, by virtue of the gaps between the network of fibres, air to pass through the dressing to the wound and pus and other detritus to pass from the wound, while preventing ingress of bacterial matter into the wound.
By controlling the diameters of the fibres in the manner described above and/or by controlling the number of layers of fibres, dressings having a range of thickness, fluid permeability and mechanical strength can be formed enabling the dressing to be adapted for use on different types of wounds and burns including wounds arising from severe trauma such as say motor vehicle accidents, battle wounds etc, and chronic wounds including lesions such as ulcerated veins as well as, where appropriate, surgically exposed tissue. The permeability of the dressing has been found to be a function of the diameters and spacing of the fibres and the motion of the nozzle over the deposition area during application.
Liquids which form short fibrils or solid droplets will not generally form a cohesive mat or web of fibres. However, liquids which form fibrils or solid droplets may be used in combination with conventional dressings or with dressings formed by fibres as discussed above, for example fibrils or solid droplets produced using a method embodying the invention may be deposited into or on a wound and then covered with one or more layers of fibres produced by method embodying the invention or by a conventional dressing.
Fibres, fibrils or droplets produced by a method embodying the invention may be deposited onto a substrate, such as a dressing, for later application to the skin, a wound, burn or the like.
Experiments have been carried out with a number of different polymers and solvents. It has been found that long chain heavy molecular structures facilitate fibre production while short chain length molecular structures tend to form fragments or solid droplets. Solvents which evaporate quickly during the jet flow may be used to facilitate formation of fibres. Suitable solvents may be, for example, methanol, propanol and water, methylene chloride, acetone and chloroform, depending upon the particular polymer used.
Experiments have been carried out in which the apparatus shown in
Fibres have been successfully spun with polyhydroxybutyric acid, a bioresorbable polymer, and polyvinyl alcohol (PVA), a polymer soluble in water and alcohols such as methanol or propanol, and pharmaceutical preparations for wound care, such as “New Skin” (trade mark) marketed by SmithKline Beecham which comprises nitrocellulose in an organic solution (in particular it comprises ethyl acetate, isopropyl alcohol, amyl acetate, isobutyl alcohol, denatured alcohol, camphor and nitrocellulose). “New Skin” is normally applied to scratches and light wounds with a rod or paddle because it is too viscous to be applicable by conventional spray devices. “New Skin” has however been successfully sprayed by a method embodying the invention to form fibres of approximately 0.5 to 5 micrometres diameter which deposited uniformly onto skin, resulting in a firm skin-like web-film. In one specific example neat (that is undiluted) “New Skin” was supplied at a flow rate of 4 millilitres per hour to a capillary nozzle of the type shown in
Polyvinyl alcohol (PVA) has also been deposited in a similar manner to the “New Skin”, using combinations of alcohol and water as solvent. Neat, undiluted PVA having a molecular weight of typically 15000 has been found to tend to form solid droplets when electrohydrodynamically processed while PVA having a molecular weight of about 140000 or more tends to form fibres. Low molecular weight PVA in a volatile solvent such as ethanol tends to break up into fibrils rather than continuous fibres. Thus, PVA having a molecular weight in the region of about 90000 to 140000 will tend to form fibrils and PVA fibrils having diameters of a few hundred nanometres and lengths of 0.5 to 10 mm have been produced.
In another experiment, an annular nozzle of the type shown in
In another example, the apparatus shown in
The fibres deposit readily onto capacitive or earthed surfaces without any of the normal problems of applying very low mass high specific surface materials and the electrical field ensures that the fibres deposit swiftly, gently and substantially uniformly.
By increasing the mass of the fibres and thus their inertia, and reducing their charge to mass ratio, greater control can be achieved over the deposition of the fibres so that the location at which the fibres are deposited on the skin or wound can be controlled mainly by moving the nozzle relative to the skin or wound and by controlling the number of passes and pattern of movement of the nozzle over the surface.
In the examples described above, the fibres, fibrils or droplets produced using the method embodying the invention consist simply of an inert polymer which may be a bioresorbable polymer such as polyhydroxybutyric acid, polyvinyl alcohol, polyglycolic acid or polylactic acid. Biologically active ingredients may, however, be added to the liquid before it is supplied to the outlet nozzle 4. In such cases, the liquid may comprise a solution, suspension, microsuspension, emulsion, microemulsion, gel or even a melt containing the active component or components. Possible active components are one or more of the following, namely pharmaceutical compounds such as analgesics, antiseptics, antibiotics, bactericides, antifungals, antiparasitics, anti-inflammatory agents, vasodilators (such as minoxidil which is believed to promote wound epithelialization and neovascularization), agents such as proteolytic enzymes for debridement and tissue repair promoting materials such as for example cytokines for stimulating cytokinetic activity to promote essential cell activities, for example to stimulate dendritic growth, growth factors such as fibroblast growth factor (FGF), epithelial growth factor (EGF), transforming growth factor (TGF) that are believed to reduce scarring and others that may be used to promote or otherwise control the sequence of events essential to natural tissue repair, cells, peptides, polypeptides, insulin, immune suppressants or stimulants and vaccines. Another possible active components are DNA or other genetic matter for gene therapy, surface binding or surface recognising agents such as surface protein A, and surfactants.
Where more than one layer of fibres, fibrils and/or particles is deposited, then different active ingredients may be provided in the different layers and different biologically active ingredients may be included in different fibres, fibrils or particles where a nozzle of the type shown in
The active ingredient may comprise an adjuvant that is a pharmacological agent added to a drug to increase or aid its effect or an immunological agent that increases the antigenic response.
Where the resulting material is in a form of fibrils, the fibrils may actually stick into the surface, for example skin or soft tissue, onto which they are deposited so enabling, for example, the supply of drugs and other biologically active agents beneath the skin or into the soft tissue, and may for example be used to carry DNA to cells.
The reservoir 20a coupled to the inner outlet 44 may contain a supply of a biologically active ingredient such as a pharmaceutical or a solution of DNA for example, while the reservoir 20b coupled to the outer nozzle 45 may contain a supply of a polymer solution of the type discussed above, for example polyhydroxybutyric acid dissolved in methylene chloride. The device shown in
By appropriate selection the molecular weight of the polymer and/or the volatility of the polymer solution, the liquids issuing from the combined nozzle can be caused to form a fibre or fibrils in which the biologically active ingredient forms a cylindrical centre core of the fibre or fibril or micro capsules in which the biologically active ingredient is completely encapsulated within the polymer and may still be in a liquid form.
For microcapsule formation it has been found preferable to reduce the percentage polymer in solution and to use a much reduced molecular weight polymer. For example a resin such as neoprene chlorinated rubber dissolved at more than about 10% by weight in trichloroethane will tend to spray fibres. However by decreasing the percentage polymer and/or using a less volatile solvent such as, for example, propylene glycol ether, microcapsules may be formed. Microcapsules have been produced using PVA of low molecular weight, for example a molecular weight of about 15000, dissolved to a dilution of between about 2.5 percent and 5 percent by volume in water or alcohol with a flow rate of about 1.0 microlitres per second. Production of microcapsules may be enhanced by using two reactive monomers one of which is placed in each of the two liquids to react during comminution.
The composite products produced using the device shown in
Composite products made up of three or more different layers of material may be formed by increasing the number of coaxial nozzles.
The outlet nozzle of the device shown in
It will, of course, be appreciated that the apparatus shown in
As discussed above, the nozzle shown in
The nozzle shown in
As another possibility the device shown in
A method embodying the invention may also be used to produce material capable of transfecting resident cells in situ with genetic material in order to regulate cell responses. For example, a method embodying the invention may be used to produce microcapsules comprising DNA encapsulated in a microcapsules or complexed with an appropriate lipid material for transfecting cells. Phospholipid microcapsules encapsulating DNA may be produced by a method embodying the invention. Other biological material such as proteins may be similarly encapsulated or complexed with an appropriate lipid material. Proteins may also be incorporated in the lipid layer. Surface binding or surface recognising agents such as surface protein A may be incorporated into microcapsules, especially phospholipid microcapsules, for selecting targets such as cancer cells, epithelial cells etc. Also, surfactants such as soya lecithin available from Sigma Pharmaceuticals may be incorporated in the outer surface of fibres, microcapsules or fibrils.
Fibres, fibrils or droplets or capsules produced by a method embodying the invention may be coated with substances such as surfactants such as soya lecithin or with, for example, DNA which is relatively sticky. This may be achieved by, for example, supplying the polymer containing liquid to the inner nozzle in
The material for oral delivery may comprise liposome encapsulated or complexed DNA for transfecting cells or may, for example comprise biologically active ingredients such as peptides, polypeptides and other large biomolecules such as insulin or growth factor, and active pharmaceutical components for enabling delivery of the active component into the blood stream via the lung. This should provide a quicker route to the bloodstream than that provided by normal oral ingestion and avoids the need for injection of components which cannot be taken orally because of the gastric enzymes and acids present in the digestive system.
Where a method embodying the invention is used to produce fibres, fibrils or microcapsules comprising a core of an active ingredient, the choice of coating material, the permeability and/or thickness of the coating may be adjusted to adjust the timing of release of the active ingredient. For example where the coating comprises a bioresorbable or biodegradable polymer, the half-life of the polymer may be controlled by controlling the permeability and/or thickness of the polymer coating by, for a specific formulation, controlling the flow rate and voltage.
A method embodying the invention may also be used to supply material to body cavities other than the respiratory system. Generally, for such use, the material will be at least partially electrically discharged before supply and means may be provided for forming an air or inert gas flow to assist the supply of the material to the body cavity. Where the body cavity is not easily accessible from the outside of the body, then the device embodying the invention may be mounted to an endoscope or like instrument enabling the device to be inserted into the body and to be positioned at the site where the material is required. The material may comprise any of the fibres, fibrils, particles and microcapsules mentioned above.
A method embodying the invention may also be used in a production process to form fibrils or particles comprising a biologically active ingredient and/or fibrils or microcapsules having a core of a biologically active ingredient which may themselves be encapsulated in conventional orally ingestible capsules, enabling, especially in the case of microcapsules, good control over the release of the active material.
A method and device embodying the invention may also be used for non-medical purposes. For example, coatings of fibres, fibrils, particles or microcapsules may be formed on substrates such as paper with good control of the thickness and uniformity of the coating. For example, adhesive may be deposited onto a substrate using a method embodying the invention.
Materials formed of two or more components which have only a short-shelf life when mixed together may be formed in a timely manner using a method embodying the present invention by encapsulating the respective components in respective fibres, fibrils or microcapsules so that mixing of the various components only occurs when the components are released from the encapsulating material by, for example, leaching through the encapsulating material, rupture by pressure being applied to the encapsulating material, temperature, or degradation, for example bioresorption or biodegradation, of the encapsulant. Such a method may be used to form, for example, two component adhesives which may be applied separately or simultaneously to a surface as cored fibres, fibrils or microcapsules by a method embodying the invention.
Other materials such as perfumes, insecticides, aromas, vapours, inks, dyes, lubricants, insect repellents etc., may be encapsulated in fibres, fibrils or microcapsules and deposited on a surface using a method embodying the invention, allowing the encapsulated ingredient to be released in a time-controlled manner as discussed in the previous paragraph, for example by application of pressure to the surface.
A method embodying the invention may also be used to produce a protective coating which may contain an active protective ingredient such as an anti-corrosive or a lubricant. For example, temporary protective coatings of delicate articles or articles liable to corrosion may be provided by depositing a web or mat on the surface of the article using a method embodying the invention.
Webs or mats formed using a method embodying the invention may also be sprayed or deposited over, for example, delicate crops such as grapes or strawberries so as to protect them from environmental effects such as frost, sun-damage, etc. Such a web may incorporate active ingredients such as insecticides, fungicides, miticides and the like to further protect the crop.
Generally, the capacitive nature of materials such as skin and the moisture content of the air should be sufficient for deposition onto a surface to occur simply by electrostatic attraction. However, where it is desired to deposit a large amount of material, then it may be necessary to earth the surface or to maintain it at a lower or opposite potential to the charged matter.
A method and device embodying the invention may also be used for supplying material to cavities other than body cavities and to concave surfaces. In such circumstances, the charged matter will generally be at least partially electrically discharged before it reaches the cavity or concave surface.
As used herein, the term “particle” includes solid, partially solid and gel-like droplets and microcapsules which incorporate solid, partially solid, gel-like or liquid matter. The term “active ingredient” means material which is compatible with and has an affect on the substrate or body to which it is to be applied and the term “biologically active ingredient” or “biologically active material” means material which is compatible with and has an affect (which may for example be biological, chemical or biochemical) on the animal or plant to which it is to be applied and includes, for example, medicaments such as proprietary medicines, pharmaceutical medicines and veterinary medicines, vaccines, genetic material such as DNA, cells and the like.
Claims
1-43. (canceled)
44. A hand holdable wound care device that enables a user to apply a dressing onto a wound surface, comprising a housing adapted to be held in a user's hand, the housing having a housing outlet, the housing containing:
- a reservoir containing liquid and having a reservoir outlet;
- a liquid supply tube configured to receive liquid from the reservoir outlet and having a supply tube outlet registering with the housing outlet;
- a pump for pumping liquid from the reservoir along the liquid supply tube to the supply tube outlet and through the housing outlet;
- a voltage source for supplying voltage; and
- user operable switch means adapted to be activated manually by the user, the housing outlet being directable by the user towards the wound surface so as to couple the voltage supplied by the voltage source to the supply tube outlet to generate an electric field to cause the liquid to form at the housing outlet at least one electrically charged jet, the jet forming a fiber that is eletrostatically attracted to and deposits on the wound surface to form a mat or web of fiber providing a dressing covering the wound surface.
45-49. (canceled)
50. A method of promoting or controlling tissue repair in a wound, the method comprising:
- subjecting liquid issuing from an outlet to an electric field to cause the liquid to form at least one jet of electrically charged liquid and from the at least one jet at least one electrically charged fiber which, by virtue of its charge, is attracted to and deposits on the wound to form a mat or web; and
- incorporating into the mat or web cytokines for stimulating cytokinetic activity to promote cell activities to stimulate dendritic growth.
51. A method of providing a tissue repair promoting dressing for a wound, the method comprising:
- providing a hand holdable device comprising a housing adapted to be held in the user's hand and having a housing outlet and containing a reservoir containing liquid comprising a biologically compatible polymer and having a reservoir outlet, a liquid supply tube for receiving liquid from the reservoir outlet and having a supply tube outlet registered with the housing outlet, and a voltage source for applying a voltage to generate an electric field at the supply tube outlet, the method comprising:
- positioning the housing outlet at least 5 [to 10] cm away form the wound;
- activating the voltage source to cause the liquid issuing from the supply tube outlet to form at least one electrically charged fiber having a diameter in the range of 10 nanometers to 10 micrometers which fiber, by virtue of its charge, is attracted to and deposits onto the wound to form a mat or web with adjacent fiber portions being spaced apart by a gap; and
- directing a spray containing an active ingredient comprising cytokines for promoting tissue repair onto the web or mat.
52. A method of promoting or controlling tissue repair, the method comprising:
- controlling a supply of liquid from an outlet and subjecting the liquid issuing from the outlet to an electric field to cause the liquid to form electrically charged fibers having a diameter in a region of 10 nanometers to 10 micrometers and having a charge-to-mass ratio such that the fibers are attracted to and deposit onto the wound to form a mat or web having a controlled pattern of fibers; and
- incorporating into the mat or web an active ingredient that promotes tissue repair to reduce scarring or otherwise control the sequence of events essential to natural tissue repair.
53. A method according to claim 52, which comprises incorporating the active ingredient into the mat or web by electrohydrogenatomically spraying a liquid comprising at least one tissue repair promoting active ingredient selected form a group consisting of cytokines that stimulate cytokinetic activity to promote cell activity and growth factors that include fibroblast growth factor, epithelial growth factor and transforming growth factor.
54. A method according to claim 52, which comprises incorporating the active ingredient by providing the active ingredient within the liquid supply to the liquid outlet.
55. A hand holdable device for enabling promotion of tissue repair, the device comprising a housing adapted to be held in a user's hand, the housing having a housing outlet, the housing containing:
- a reservoir containing liquid comprising a biologically compatible polymer and having a reservoir outlet;
- a liquid supply tube for receiving liquid from the reservoir outlet and having a supply tube outlet registering with the housing outlet;
- a voltage source for applying a voltage; a spray device comprising a second reservoir containing liquid comprising an active ingredient and having an outlet for spraying liquid containing the active ingredient into the path of liquid issuing from the liquid supply tube; and
- user operable switch means adapted to be activated by a user when the device is held in the user's hand to cause an electric field to be generated at the supply tube outlet to cause liquid from the reservoir to form at the housing outlet at least one electrically charged fiber and to cause liquid containing the active ingredient to be sprayed onto the fiber whereby, in use, the fiber is electrostatically attracted to and deposits onto the wound to form a mat or web incorporating the active ingredient sprayed by the spraying device.
56. A method of promoting tissue repair, which method comprises generating electrostatically charged fibers by subjecting liquid containing a biologically compatible polymer issuing from an outlet to an electric field and controlling the charge ratio of the fiber thereby enabling a location at which the fibers are deposited on the wound to be controlled mainly by moving the liquid outlet relative to the wound and controlling a number of passes and pattern of movement of the outlet over the wound.
57. A method of providing a fiber covering on a surface, the method comprising:
- providing a reservoir of a polymer containing liquid to a liquid outlet via a supply tube; and
- subjecting liquid issuing from the liquid outlet to an electric field while regulating a flow of liquid along the supply tube to the liquid outlet to cause the liquid to form at least one electrically charge jet which forms at least one electrically charged fiber that has a diameter controlled by the flow rate and which is electrically attracted to and deposits onto the surface.
58. A method of supplying an active ingredient to an area of skin or soft tissue, the method comprising:
- providing a polymer containing liquid comprising an active ingredient to an outlet directed towards said area; and
- subjecting the liquid that issues from the outlet to an electric field to cause the liquid to form at least one electrically charged jet that breaks up to provide the charged fiber fragments that are electrically attracted to and stick onto said area of skin or soft tissue to supply the active ingredient beneath the skin or into the soft tissue.
59. (canceled)
60. A method of producing a fibrous layer covering on a surface, the method comprising:
- supplying a polymer containing liquid to a liquid outlet nozzle;
- subjecting liquid issuing from the liquid outlet nozzle to an electric field while regulating the flow of the liquid to the liquid outlet nozzle such that liquid issuing from the liquid outlet nozzle forms a multitude of liquid jets each of which forms an electrically charged fiber of breaks up into fibrils or particles which are electrically attracted to and deposit onto the surface.
61. (canceled)
62. A method of depositing material into a cavity or onto a concave surface, which method comprises:
- supplying polymer containing liquid to a liquid outlet directed towards the cavity or concave surface; and
- subjecting liquid issuing from the liquid outlet to an electric field thereby causing the liquid to form at least one jet of electrically charged liquid which then forms electrically charged polymer matter comprising at least one of an electrically charged polymer fiber, electrically charged polymer fiber fragments or electrically charged polymer particles.
63. A method of producing material for supply to the respiratory system of a mammal, which method comprises:
- supplying a polymer containing liquid to a liquid outlet; and
- generating at the liquid outlet an electrical field to cause the polymer containing liquid to form at least one liquid jet which forms charged polymer matter comprising at least one of polymer fiber, fiber fragments and polymer particles.
64. A method of forming a cavity wound dressing, which method comprises:
- supplying a first liquid comprising thrombin to a first liquid outlet;
- supplying a second liquid comprising fibrinogen to a second liquid outlet;
- subjecting liquid issuing from the first and second liquid outlets to an electric field so as to generate liquid droplets of opposite polarity which rapidly coalesce causing the thrombin and fibrinogen to react together to form a fibrin mat in the wound cavity.
65-80. (canceled)
81. A method of forming a hemostatic dressing, said method comprising electrohydrodynamically processing liquid comprising at least one active ingredient selected from fibrinogen, thrombin and mixtures thereof, to form a fibrous mat.
82. The method claim 81 wherein said liquid comprises at least one additional active ingredient selected from the group consisting of analgesics, antiseptics, antibiotics, bactericides, anti fungals, antiparasitics, anti-inflammatory agents, vasodilators, proteolytic enzymes, cytokines, fibroblast growth factor (FGF), epithelial growth factor (EGF), transforming growth-factor (TGF), cells, peptides, polypeptides, insulin, immune suppressants, stimulants, vaccines, and mixtures thereof.
83. The method claim 81 wherein the method further comprises electrohydrodynamically processing thrombin at one polarity so as to rapidly coalesce with droplets of opposite polarity comprising fibrinogen to form a fibrin mat.
84. The method of claim 81 wherein at least a portion of the fibers include collagen, and at least a portion of the fibers include at least one active component selected from thrombin, fibrinogen and mixtures thereof.
85. The method claim 81 wherein the step of electrohydrodynamically processing comprises processing matter comprising thrombin at one polarity so as to rapidly coalesce with matter of opposite polarity comprising fibrinogen to form a fast-reacting fibrin mat.
86. A wound dressing formed by supplying liquid to at least one outlet; subjecting liquid at an outlet to an electric field thereby causing the liquid to form at least one jet of electrically charged liquid, the liquid being such that after formation the at least one jet forms charged fibers which deposit onto a surface to form a mat; and the fibers comprise a bioresorbable inert polymer.
87. The wound dressing of claim 86 wherein the fibers further comprise one or more active components.
88. The wound dressing of claim 87 wherein the active components are selected form the group consisting of analgesics, antiseptics, antibiotics, bactericides, antifungals, antiparasitics, anti-inflammatory agents, fibrinogen, vasodilators, proteolytic enzymes, cytokines, fibroblast growth factor (FGF), epithelial growth factor (EGF), thrombin, transforming growth factor (TGF), cells, peptides, polypeptides, insulin, immune suppressants, stimulants, vaccines, and mixtures thereof.
89. The wound dressing of claim 88 wherein the active components are selected from fibrinogen, thrombin, and mixtures thereof.
90. The wound dressing of claim 86 wherein at least a portion of the fibers include collagen, and at least a portion of the fibers include at least one active component selected from thrombin, fibrinogen and mixtures thereof.
91. The wound dressing of claim 86 wherein the polymer is selected from the group consisting of polyhydroxybutyric, polyvinyl alcohol, polyglycolic acid, polylactic acid, and mixtures thereof.
92. The wound dressing of claim 86 wherein the dressing comprises more than one layer of fibers.
93. The wound dressing of claim 92 wherein skin cells are interspersed between fiber layers.
94. The wound dressing of claim 86 wherein the mat is formed by electrohydrodynamically processing thrombin at one polarity so as to rapidly coalesce with matter of opposite polarity comprising fibrinogen to form a fast-reacting fibrin mat.
95. The wound dressing of claim 86 comprised of more than one layer.
96. The wound dressing of claim 86 wherein cells are dispersed between the layers.
97. A pre-formed, stable hemostatic dressing comprising fibers wherein the dressing is formed by subjecting to an electric field a liquid comprising an active ingredient selected from fibrinogen, thrombin, and mixtures thereof.
98. The dressing of claim 97 wherein said liquid is processed by EHD processing.
99. The dressing of claim 98 wherein the fibers comprise a bioresorbable polymer.
100. The dressing of claim 99 wherein the polymer is selected from the group consisting of polyhydroxybutyric acid, polyvinyl alcohol, polyglycolic acid, polylactic acid, and mixtures thereof.
101. The dressing of claim 99 wherein the fibers comprise an additional active ingredient selected from the group consisting of analgesics, antiseptics, antibiotics, bactericides, antifungals, antiparasitics, anti-inflammatory agents, vasodilators, proteolytic enzymes, cytokines, fibroblast growth factor (FGF), epithelial growth factor (EGF), transforming growth factor (TGF), cells, peptides, polypeptides, insulin, immune suppressants, stimulants, vaccines, and mixtures thereof.
102. The dressing of claim 99 wherein said dressing is adapted for application to an external surface.
103. The dressing of claim 99 wherein said dressing is adapted for application to an internal surface.
104. A method of forming a product containing an active ingredient, the method comprising supplying a liquid containing an active ingredient to an outlet and subjecting the liquid to an electric field to cause formation of electrically charged particles of a bioresorbable or biodegradable polymer carrying the active ingredient whereby, when the particles are contacted by chemicals or enzymes present in biological tissue or fluid, the polymer is degraded by chemical or enzymic attack to enable release of the active ingredient.
Type: Application
Filed: Feb 27, 2007
Publication Date: May 22, 2008
Inventor: Ronald Alan Coffee (Surrey)
Application Number: 11/711,378
International Classification: A61F 13/00 (20060101);