Metalloprotease inhibitors containing a heterocyclic moiety

The present invention relates generally to pharmaceutical agents containing a heterocyclic moiety, and in particular, to heterocyclic metalloprotease inhibiting compounds. More particularly, the present invention provides a new class of heterocyclic MMP-3, MMP-8 and/or MMP-13 inhibiting compounds with a squaramide or benzoxazinone moiety, that exhibit an increased potency and selectivity in relation to currently known MMP-13, MMP-8 and MMP-3 inhibitors.

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Description
CROSS REFERENCE TO RELATED APPLICATIONS

This application claims the benefit of U.S. Provisional Application No. 60/905,565, filed Mar. 7, 2007, which is hereby incorporated by reference.

FIELD OF THE INVENTION

The present invention relates to a new class of heterocyclic moiety containing pharmaceutical agents which inhibits metalloproteases. In particular, the present invention provides a new class of metalloprotease inhibiting compounds containing a benzoxazinone moiety that exhibit potent inhibiting activity towards metalloproteases, in particular towards MMP-13, MMP-3, MMP-8, and more particulary towards MMP-13.

BACKGROUND OF THE INVENTION

Matrix metalloproteinases (MMPs) and aggrecanases (ADAMTS=a disintegrin and metalloproteinase with thrombospondin motif) are a family of structurally related zinc-containing enzymes that have been reported to mediate the breakdown of connective tissue in normal physiological processes such as embryonic development, reproduction, and tissue remodelling. Over-expression of MMPs and aggrecanases or an imbalance between extracellular matrix synthesis and degradation has been suggested as factors in inflammatory, malignant and degenerative disease processes. MMPs and aggrecanases are, therefore, targets for therapeutic inhibitors in several inflammatory, malignant and degenerative diseases such as rheumatoid arthritis, osteoarthritis, osteoporosis, periodontitis, multiple sclerosis, gingivitis, corneal epidermal and gastric ulceration, atherosclerosis, neointimal proliferation (which leads to restenosis and ischemic heart failure) and tumor metastasis.

The ADAMTSs are a group of proteases that are encoded in 19 ADAMTS genes in humans. The ADAMTSs are extracellular, multidomain enzymes whose functions include collagen processing, cleavage of the matrix proteoglycans, inhibition of angiogenesis and blood coagulation homoeostasis (Biochem. J. 2005, 386, 15-27; Arthritis Res. Ther. 2005, 7, 160-169; Curr. Med. Chem. Anti-Inflammatory Anti-Allergy Agents 2005, 4, 251-264).

The mammalian MMP family has been reported to include at least 20 enzymes (Chem. Rev. 1999, 99, 2735-2776). Collagenase-3 (MMP-13) is among three collagenases that have been identified. Based on identification of domain structures for individual members of the MMP family, it has been determined that the catalytic domain of the MMPs contains two zinc atoms; one of these zinc atoms performs a catalytic function and is coordinated with three histidines contained within the conserved amino acid sequence of the catalytic domain. MMP-13 is over-expressed in rheumatoid arthritis, osteoarthritis, abdominal aortic aneurysm, breast carcinoma, squamous cell carcinomas of the head and neck, and vulvar squamous cell carcinoma. The principal substrates of MMP-13 are fibrillar collagens (types I, II, III) and gelatins, proteoglycans, cytokines and other components of ECM (extracellular matrix).

The activation of the MMPs involves the removal of a propeptide, which features an unpaired cysteine residue complexed with the catalytic zinc (II) ion. X-ray crystal structures of the complex between MMP-3 catalytic domain and TIMP-1 and MMP-14 catalytic domain and TIMP-2 also reveal ligation of the catalytic zinc (II) ion by the thiol of a cysteine residue. The difficulty in developing effective MMP inhibiting compounds comprises several factors, including choice of selective versus broad-spectrum MMP inhibitors and rendering such compounds bioavailable via an oral route of administration.

MMP-3 (stromelysin-1; transin-1) is another member of the MMP family (FASEB J. 1991, 5, 2145-2154). Human MMP-3 was initially isolated from cultured human synoviocytes. It is also expressed by chondrocytes and has been localized in OA cartilage and synovial tissues (Am. J. Pathol. 1989, 135, 1055-64).

MMP-3 is produced by basal keratinocytes in a variety of chronic ulcers. MMP-3 mRNA and Protein were detected in basal keratinocytes adjacent to but distal from the wound edge in what probably represents the sites of proliferating epidermis. MMP-3 may thus prevent the epidermis from healing (J. Clin. Invest. 1994, 94, 79-88).

MMP-3 serum protein levels are significantly elevated in patients with early and long-term rheumatoid arthritis (Arthritis Rheum. 2000, 43, 852-8) and in osteoarthritis patients (Clin. Orthop. Relat. Res. 2004, 428, 272-85) as well as in other inflammatory diseases like systemic lupus erythematosis and ankylosing spondylitis (Rheumatology 2006, 45, 414-20).

MMP-3 acts on components of the ECM as aggrecan, fibronectin, gelatin, laminin, elastin, fibrillin and others and on collagens of type III, IV, V, VII, IX, X (Clin. Orthop. Relat. Res. 2004, 428,272-85). On collagens of type II and IX, MMP-3 exhibits telopeptidase activity (Arthritis Res. 2001, 3, 107-13; Clin. Orthop. Relat. Res. 2004, 427, S118-22). MMP-3 can activate other MMP family members such as MMP-1, MMP-7, MMP-8, MMP-9 and MMP-13 (Ann. Rheum. Dis. 2001, 60 Suppl 3:iii62-7).

MMP-3 is involved in the regulation of cytokines and chemokines by releasing TGFβ1 from the ECM, activating TNFα, inactivating IL-1β and releasing IGF (Nat. Rev. Immunol. 2004, 4, 617-29). A potential role for MMP-3 in the regulation of macrophage infiltration is based on the ability of the enzyme to convert active MCP species into antagonistic peptides (Blood 2002, 100, 1160-7).

MMP-8 (collagenase-2; neutrophil collagenase; EC 3.4.24.34) is another member of the MMP family (Biochemistry 1990, 29, 10628-34). Human MMP-8 was initially located in human neutrophils (Biochemistry 1990, 29, 10620-7). It is also expressed by macrophages, human mucosal keratinocytes, bronchial epithelial cells, ginigival fibroblasts, resident synovial and articular chondrodrocytes mainly in the course of inflammatory conditions (Cytokine & Growth Factor Rev. 2006, 17, 217-23).

The activity of MMP-8 is tightly regulated and mostly limited to the sites of inflammation. MMP-8 is expressed and stored as an inactive pro-enzyme in the granules of the neutrophils. Only after the activation of the neutrophils by proinflammatory mediators, MMP-8 is released and activated to exert its function.

MMP-8 plays a key role in the migration of immune cells to the sites of inflammation. MMP-8 degrades components of the extracellular matrix (ECM) such as collagen type I, II, III, VII, X, cartilage aggrecan, laminin-5, nidogen, fibronectin, proteoglycans and tenascin, thereby facilitating the cells migration through the ECM barrier. MMP-8 also influences the biological activity of its substrates. Through proteolytic processing of the chemokines IL-8, GCP-2, ENA-78, MMP-8 increases the chemokines ability to activate the infiltrating immune cells. While MMP-8 inactivates the serine protease inhibitor alpha-1 antitrypsin through its cleavage (Eur. J Biochem. 2003, 270, 3739-49; PloS One 2007, 3, 1-10; Cytokine & Growth Factor Rev. 2006, 17, 217-23).

MMP-8 has been implicated in the pathogenesis of several chronic inflammatory diseases characterized by the excessive influx and activation of neutrophils, including cystic fibrosis (Am. J. Resprir. Critic. Care Med. 1994, 150, 818-22), rheumatoid arthritis (Clin. Chim. Acta 1996, 129-43), chronic periodontal disease (Annals Med. 2006, 38, 306-321) and chronic wounds (J. Surg. Res. 1999, 81, 189-195).

In osteoarthritis patients, MMP-8 protein expression is significantly elevated in inflamed human articular cartilage in the knee and ankle joints (Lab Invest. 1996, 74, 232-40; J. Biol. Chem. 1996, 271, 11023-6).

The levels of activated MMP-8 in BALF is an indicator of the disease severity and correlates with the airway obstruction in patients with asthma, COPD, pulmonary emphysema and bronchiectasis (Lab Invest. 2002, 82, 1535-45; Am. J. Respir. Crit. Care Med. 1999, 159, 1985-91; Respir. Med. 2005, 99, 703-10; J Pathol. 2001, 194, 232-38).

SUMMARY OF THE INVENTION

The present invention relates to a new class of heterocyclic moiety containing pharmaceutical agents which inhibits metalloproteases. In particular, the present invention provides a new class of metalloprotease inhibiting compounds that exhibit potent inhibiting activity towards metalloproteases, in particular towards MMP-13, MMP-3, MMP-8, and more particulary towards MMP-13.

The present invention provides a new classes of heterocyclic metalloprotease compounds, which is represented by the following general formula:

wherein all variables in the preceding Formulas (I) are as defined hereinbelow.

The heterocyclic metalloprotease inhibiting compounds of the present invention may be used in the treatment of metalloprotease mediated diseases, such as rheumatoid arthritis, osteoarthritis, abdominal aortic aneurysm, cancer (e.g. but not limited to melanoma, gastric carcinoma or non-small cell lung carcinoma), inflammation, atherosclerosis, multiple sclerosis, chronic obstructive pulmonary disease, ocular diseases (e.g. but not limited to ocular inflammation, glaucoma, retinopathy of prematurity, macular degeneration with the wet type preferred and corneal neovascularization), neurologic diseases, psychiatric diseases, thrombosis, bacterial infection, Parkinson's disease, fatigue, tremor, diabetic retinopathy, vascular diseases of the retina, aging, dementia, cardiomyopathy, renal tubular impairment, diabetes, psychosis, dyskinesia, pigmentary abnormalities, deafness, inflammatory and fibrotic syndromes, intestinal bowel syndrome, allergies, Alzheimers disease, arterial plaque formation, oncology, periodontal, viral infection, stroke, atherosclerosis, cardiovascular disease, reperfusion injury, trauma, chemical exposure or oxidative damage to tissues, chronic wound healing, wound healing, hemorroid, skin beautifying, pain, inflammatory pain, bone pain and joint pain, acne, acute alcoholic hepatitis, acute inflammation, acute pancreatitis, acute respiratory distress syndrome, adult respiratory disease, airflow obstruction, airway hyperresponsiveness, alcoholic liver disease, allograft rejections, angiogenesis, angiogenic ocular disease, arthritis, asthma, atopic dermatitis, bronchiectasis, bronchiolitis, bronchiolitis obliterans, burn therapy, cardiac and renal reperfusion injury, celiac disease, cerebral and cardiac ischemia, CNS tumors, CNS vasculitis, colds, contusions, cor pulmonae, cough, Crohn's disease, chronic bronchitis, chronic inflammation, chronic pancreatitis, chronic sinusitis, crystal induced arthritis, cystic fibrosis, delayted type hypersensitivity reaction, duodenal ulcers, dyspnea, early transplantation rejection, emphysema, encephalitis, endotoxic shock, esophagitis, gastric ulcers, gingivitis, glomerulonephritis, glossitis, gout, graft vs. host reaction, gram negative sepsis, granulocytic ehrlichiosis, hepatitis viruses, herpes, herpes viruses, HIV, hypercapnea, hyperinflation, hyperoxia-induced inflammation, hypoxia, hypersensitivity, hypoxemia, inflammatory bowel disease, interstitial pneumonitis, ischemia reperfusion injury, kaposi's sarcoma associated virus, liver fibrosis, lupus, malaria, meningitis, multi-organ dysfunction, necrotizing enterocolitis, osteoporosis, chronic periodontitis, periodontitis, peritonitis associated with continous ambulatory peritoneal dialysis (CAPD), pre-term labor, polymyositis, post surgical trauma, pruritis, psoriasis, psoriatic arthritis, pulmatory fibrosis, pulmatory hypertension, renal reperfusion injury, respiratory viruses, restinosis, right ventricular hypertrophy, sarcoidosis, septic shock, small airway disease, sprains, strains, subarachnoid hemorrhage, surgical lung volume reduction, thrombosis, toxic shock syndrome, transplant reperfusion injury, traumatic brain injury, ulcerative colitis, vasculitis, ventilation-perfusion mismatching, and wheeze.

In particular, the heterocyclic metalloprotease inhibiting compounds of the present invention may be used in the treatment of MMP-13, MMP-8 and MMP-3 mediated degenerative diseases characterized by excessive extracellular matrix degradation and/or remodelling, such as cancer, and chronic inflammatory diseases such as arthritis, rheumatoid arthritis, osteoarthritis, atherosclerosis, abdominal aortic aneurysm, inflammation, multiple sclerosis, parkinsons disease, chronic obstructive pulmonary disease and pain, such as inflammatory pain, bone pain and joint pain.

The present invention also provides heterocyclic metalloprotease inhibiting compounds that are useful as active ingredients in pharmaceutical compositions for treatment or prevention of metalloprotease—especially MMP-13—mediated diseases. The present invention also contemplates use of such compounds in pharmaceutical compositions for oral or parenteral administration, comprising one or more of the heterocyclic metalloprotease inhibiting compounds disclosed herein.

The present invention further provides methods of inhibiting metalloproteases, by administering formulations, including, but not limited to, oral, rectal, topical, intravenous, parenteral (including, but not limited to, intramuscular, intravenous), ocular (ophthalmic), transdermal, inhalative (including, but not limited to, pulmonary, aerosol inhalation), nasal, sublingual, subcutaneous or intraarticular formulations, comprising the heterocyclic metalloprotease inhibiting compounds by standard methods known in medical practice, for the treatment of diseases or symptoms arising from or associated with metalloprotease, especially MMP-13, including prophylactic and therapeutic treatment. Although the most suitable route in any given case will depend on the nature and severity of the conditions being treated and on the nature of the active ingredient. The compounds from this invention are conveniently presented in unit dosage form and prepared by any of the methods well-known in the art of pharmacy.

The heterocyclic metalloprotease inhibiting compounds of the present invention may be used in combination with a disease modifying antirheumatic drug, a nonsteroidal anti-inflammatory drug, a COX-2 selective inhibitor, a COX-1 inhibitor, an immunosuppressive, a steroid, a biological response modifier, a viscosupplement, a pain reducing drug or other anti-inflammatory agents or therapeutics useful for the treatment of chemokines mediated diseases.

DETAILED DESCRIPTION OF THE INVENTION

One aspect of the invention relates to a compound having Formula (I):

wherein:

R4 in each occurrence is independently selected from R10, hydrogen, alkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, halo, haloalkyl, CF3, (C0-C6)-alkyl-COR10, (C0-C6)-alkyl-OR10, (C0-C6)-alkyl-NR10R11, (C0-C6)-alkyl-NO2, (CO-C6)-alkyl-CN, (C0-C6)-alkyl-S(O)yOR10, (C0-C6)-alkyl-S(O)yNR10OR11, (C0-C6)-alkyl-NR10CONR11SO2R30, (C0-C6)-alkyl-S(O)xR10, (C0-C6)-alkyl-OC(O)R10, (C0-C6)-alkyl-OC(O)NR10R11, (C0-C6)-alkyl-C(═NR10)NR10R11, (C0-C6)-alkyl-NR10C(═NR11)NR10R11, (C0-C6)-alkyl-C(O)OR10, (C0-C6)-alkyl-C(O)NR10R11, (C0-C6)-alkyl-C(O)NR10SO2R11, (C0-C6)-alkyl-C(O)—NR11—CN, O—(C0-C6)-alkyl-C(O)NR1R11, S(O)x—(C0-C6)-alkyl-C(O)OR10, S(O)x—(C0-C6)-alkyl-C(O)NR10R11, (C0-C6)-alkyl-C(O)NR10—(C0-C6)-alkyl-NR10R11, (C0-C6)-alkyl-NR10—C(O)R10, (C0-C6)-alkyl-NR10—C(O)OR10, (C0-C6)-alkyl-NR10—C(O)—NR10R11, (C0-C6)-alkyl-NR10—S(O)yNR10R11, (C0-C6)-alkyl-NR10—S(O)yR10, O—(C0-C6)-alkyl-aryl and O—(C0-C6)-alkyl-heteroaryl,

wherein each R4 group is optionally substituted one or more times, or

wherein each R4 group is optionally substituted by one or more R14 groups;

R8 is selected from R10 or optionally R8 and X1 when taken together with the nitrogen and sp2-carbon atom to which they are attached complete a 5- to 8-membered unsaturated or partially unsaturated heterocycle optionally containing additional heteroatoms selected from O, S(O)x, N or NR50 and which is optionally substituted one or more times;

R9 in each occurrence is independently selected from R10, hydrogen, alkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, halo, CHF2, CF3, OR10, SR10, COOR10, CH(CH3)CO2H, (C0-C6)-alkyl-COR10, (C0-C6)-alkyl-OR10, (C0-C6)-alkyl-NR10R11, (C0-C6)-alkyl-NO2, (C0-C6)-alkyl-CN, (C0-C6)-alkyl-S(O)yOR10, (C10-C6)-alkyl-P(O)2OH, (C0-C6)-alkyl-S(O)yNR10R11, (C0-C6)-alkyl-NR10CONR11SO2R30, (C0-C6)-alkyl-S(O)xR10, (C0-C6)-alkyl-OC(O)R10, (C0-C6)-alkyl-OC(O)NR10R11, (C0-C6)-alkyl-C(═NR10)NR10R11, (C0-C6)-alkyl-NR10C(═NR11)NR10R11, (C0-C6)-alkyl-NR10C(═N—CN)NR10R11, (C0-C6)-alkyl-C(═N—CN)NR10R11, (C0-C6)-alkyl-NR10C(═N—NO2)NR10R11, (C0-C6)-alkyl-C(═N—NO2)NR10OR11, (C0-C6)-alkyl-C(O)OR10, (C0-C6)-alkyl-C(O)NR10R11, (C0-C6)-alkyl-C(O)NR10SO2R11, C(O)NR10—(C0-C6)-alkyl-heteroaryl, C(O)NR10—(C0-C6)-alkyl-aryl, S(O)2NR10—(C0-C6)-alkyl-aryl, S(O)2NR10—(C0-C6)-alkyl-heteroaryl, S(O)2NR10-alkyl, S(O)2—(C0-C6)-alkyl-aryl, S(O)2—(C0-C6)-alkyl-heteroaryl, (C0-C6)-alkyl-C(O)—NR11—CN, O—(C0-C6)-alkyl-C(O)NR10R11, S(O)x—(C0-C6)-alkyl-C(O)OR10, S(O)x—(C0-C6)-alkyl-C(O)NR10R11, (C0-C6)-alkyl-C(O)NR10—(C0-C6)-alkyl-NR10R11, (C0-C6)-alkyl-NR10—C(O)R10, (C0-C6)-alkyl-NR10—C(O)OR10, (C0-C6)-alkyl-NR10—C(O)—NR10R11, (C0-C6)-alkyl-NR10—S(O)yNR10R11, (C0-C6)-alkyl-NR10l —S(O)yR11, O—(C0-C6)-alkyl-aryl and O—(C0-C6)-alkyl-heteroaryl, wherein each R9 group is optionally one or more times substituted;

R10 and R11 in each occurrence are independently selected from hydrogen, alkyl, cycloalkyl, cycloalkylalkyl, heterocycloalkyl, fluoroalkyl, heterocycloalkylalkyl, haloalkyl, alkenyl, alkynyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl and aminoalkyl, wherein alkyl, cycloalkyl, cycloalkylalkyl, heterocycloalkyl, fluoroalkyl, heterocycloalkylalkyl, alkenyl, alkynyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl and aminoalkyl are optionally substituted one or more times, or R10 and R11 when taken together with the nitrogen to which they are attached complete a 3- to 8-membered ring containing carbon atoms and optionally containing a heteroatom selected from O, S(O)x, or NR50 and which is optionally substituted one or more times;

R4 is independently selected from hydrogen, alkyl, arylalkyl, cycloalkylalkyl, heteroarylalkyl, heterocyclylalkyl and halo, wherein alkyl, arylalkyl, cycloalkylalkyl, heteroarylalkyl and heterocyclylalkyl are optionally substituted one or more times;

R17 is selected from R9, alkenyl, alkynyl, bicycloalkyl, heterobicycloalkyl, spiroalkyl, spiroheteroalkyl, cycloalkyl fused aryl, heterocycloalkyl fused aryl, cycloalkyl fused heteroaryl, heterocycloalkyl fused heteroaryl or a bicyclic or tricyclic fused ring system, wherein at least one ring is partially saturated, and

wherein each R17 group is optionally substituted one or more times, or

wherein each R17 group is optionally substituted one or more R9 groups;

R30 is selected from alkyl and (C0-C6)-alkyl-aryl, wherein alkyl and aryl are optionally substituted;

R50 in each occurrence is independently selected from hydrogen, alkyl, aryl, heteroaryl, C(O)R80 , C(O)NR80R81, SO2R80 and SO2NR80R81, wherein alkyl, aryl, and heteroaryl are optionally substituted one or more times;

R80 and R81 in each occurrence are independently selected from hydrogen, alkyl, cycloalkyl, cycloalkylalkyl, heterocycloalkyl, fluoroalkyl, heterocycloalkylalkyl, haloalkyl, alkenyl, alkynyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl and aminoalkyl, wherein alkyl, cycloalkyl, cycloalkylalkyl, heterocycloalkyl, fluoroalkyl, heterocycloalkylalkyl, alkenyl, alkynyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl and aminoalkyl are optionally substituted, or R80 and R81 when taken together with the nitrogen to which they are attached complete a 3- to 8-membered ring containing carbon atoms and optionally a heteroatom selected from O, S(O)x, NH, and N(alkyl) and which is optionally substituted one or more times;

Lc is selected from a single bond or an acyclic, straight or branched, saturated or unsaturated hydrocarbon chain having 1 to 10 carbon atoms, optionally containing 1 to 3 groups independently selected from —S—, —O—, NR10—, —NR10CO—, —CONR10—, —S(O)x—, —SO2NR10—, —NR10SO2—, NR10SO2NR10—, —NR10CONR10—, —OC(O)NR10—, —NR10C(O)O—, which replace single carbon atoms, which in case that more than two carbon atoms are replaced are not adjacent, and wherein the hydrocarbon chain is optionally substituted one or more times;

Ld is selected from a single bond or a straight or branched, saturated or unsaturated hydrocarbon chain having 1 to 10 carbon atoms, optionally containing 1 to 3 groups independently selected from —O—, —NR10—, —S(O)x—, —NR10C(X1)—, —C(X1I)NR10—, —SO2NR10—, —NR10SO2—, —O—SO2—, —SO2—O—, —NR10SO2NR10—, —NR10C(X1)NR10—, —OC(X1)NR10—, —NR10C(X1)O—, —OC(X1)—, —C(X1)O—, -Q2-, —NR10-Q2-, -Q2-NR10—, —C(X1)-Q2-, -Q2-C(X1)—, —O-Q2-, —S(O)1-Q2 and -Q2-S(O)x— which replace single carbon atoms, which in case that more than two carbon atoms are replaced are not adjacent, and wherein the hydrocarbon chain is optionally substituted one or more times;

Q1 is a 4- to 8-membered ring selected from cycloalkyl, heterocycloalkyl, bicycloalkyl, heterobicycloalkyl or a 5- or 6-membered ring selected from aryl and heteroaryl, wherein cycloalkyl, heterocycloalkyl, bicycloalkyl, heterobicycloalkyl, aryl and heteroaryl are optionally substituted one or more times by R4 and optionally a substituent of Q1 is linked with Ld to complete a 3- to 8-membered ring containing carbon atoms and optionally heteroatoms selected from O, S(O)x, —NH, and —N(alkyl) wherein this new ring is optionally substituted one or more times;

Q2 is independently selected from an aromatic, partially aromatic or non-aromatic cyclic, bicyclic or multicyclic system containing 0 to 8 heteroatoms selected from N, O and S(O)x, which is optionally substituted one or more times with R4 and wherein the cycles are optionally spiro fused and optionally a substituent of Q2 is linked with Ld to complete a 3- to 8-membered ring containing carbon atoms and optionally heteroatoms selected from O, S(O)x, —NH, and —N(alkyl) wherein this new ring is optionally substituted one or more times;

X1 is independently selected from O, S, NR10, NOR10, N—CN, NCOR10, N—NO2, and N—SO2R10;

Y is selected from O, S(O)x, CR10R11, and NR10;

Z1 is independently selected from C, S, S═O, PR10 and P—OR10;

w is independently selected from 0 to 3;

x is independently selected from 0 to 2;

y is selected from 1 and 2; and

N-oxides, pharmaceutically acceptable salts, prodrugs, formulations, polymorphs, racemic mixtures and stereoisomers thereof.

In one embodiment, in conjunction with any above or below embodiments, Q2-Lc-R17 is selected from:

wherein:

R5 in each occurrence is independently selected from hydrogen, alkyl, C(O)NR10R11, aryl, arylalkyl, SO2NR10R11 and C(O)OR10, wherein alkyl, aryl and arylalkyl are optionally substituted one or more times;

E is independently selected from a bond, CR10R11, O, NR5, S, S═O, S(═O)2, C(═O), N(R10)(C═O), (C═O)N(R10), N(R10)S(═O)2, S(═O)2N(R10), C═N—OR11, —C(R10R11)C(R10R11)—, —CH2—W1— and

Q3 is selected from an aromatic, partially aromatic or non-aromatic cyclic, bicyclic or multicyclic system containing 0 to 8 heteroatoms selected from N, O and S(O)x, which is optionally substituted one or more times with R4;

Q4 is selected from an aromatic, partially aromatic or non-aromatic cyclic, bicyclic or multicyclic system containing 0 to 8 heteroatoms selected from N, O and S(O)x, which is optionally substituted one or more times with R4;

U is independently selected from C(R5R10), NR5, O, S, S═O and S(═O)2;

W1 is independently selected from O, NR5, S, S═O, S(═O)2, N(R10)(C═O), N(R10)S(═O)2 and S(═O)2N(R10); and

g and h are independently selected from 0-2.

In one embodiment, in conjunction with any above or below embodiments, Q4 is selected from an aromatic, partially aromatic or non-aromatic bicyclic or multicyclic system containing 0 to 8 heteroatoms selected from N, O and S(O)x, which is optionally substituted one or more times with R4.

In one embodiment, in conjunction with any above or below embodiments, the compound is:

wherein:

R8 is selected from R10 or optionally R8 and X1 when taken together with the nitrogen and sp2-carbon atom to which they are attached complete a 5- to 8-membered unsaturated or partially unsaturated heterocycle optionally containing additional heteroatoms selected from O, S(O)x, N or NR50 and which is optionally substituted one or more times;

L, M and T are independently selected from CR9 and N;

X1 is independently selected from O, S, NR10, NOR10, N—CN, NCOR10, N—NO2, and N—SO2R10.

In one embodiment, in conjunction with any above or below embodiments, R4 is substituted 0, 1 or 2 times.

In one embodiment, in conjunction with any above or below embodiments, R4 is substituted by 0, 1 or 2 R14 groups.

In one embodiment, in conjunction with any above or below embodiments, R6 group is substituted 0, 1 or 2 times.

In one embodiment, in conjunction with any above or below embodiments, R6 group is substituted by 0, 1 or 2 R14 groups;

In one embodiment, in conjunction with any above or below embodiments, R7 is independently selected from hydrogen, alkyl, cycloalkyl, halo, R4 and NR10R11, wherein alkyl and cycloalkyl are optionally substituted one or more times, or optionally two R7 groups together at the same carbon atom form ═O, ═S or═NR10;

In one embodiment, in conjunction with any above or below embodiments, R8 is R10.

In one embodiment, in conjunction with any above or below embodiments, R8 and X1 when taken together with the nitrogen and sp2-carbon atom to which they are attached complete a 5- to 8-membered unsaturated or partially unsaturated heterocycle optionally containing additional heteroatoms selected from O, S(O)x, N or NR50 and which is substituted 0, 1 or 2 times.

In one embodiment, in conjunction with any above or below embodiments, one R9 is selected from R10, alkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, halo, CHF2, CF3, OR10, SR10, COOR10, CH(CH3)CO2H, (C0-C6)-alkyl-COR10, (C0-C6)-alkyl-OR10, (C0-C6)-alkyl-NR10R11, (C0-C6)-alkyl-NO2, (C0-C6)-alkyl-CN, (C0-C6)-alkyl-S(O)yOR10, (C0-C6)-alkyl-P(O)2OH, (C0-C6)-alkyl-S(O)yNR10R11, (C0-C6)-alkyl-NR10CONR11SO2R30, (C0-C6)-alkyl-S(O)xR10, (C0-C6)-alkyl-OC(O)R10, (C0-C6)-alkyl-OC(O)NR10R11, (C0-C6)-alkyl-C(═NR10)NR10R11, (C0-C6)-alkyl-NR10C(═NR11)NR10R11, (C0-C6)-alkyl-NR10C(═N—CN)NR10R11, (C0-C6)-alkyl-C(═N—CN)NR10R11, (C0-C6)-alkyl-NR10C(═N—NO2)NR10R11, (C0-C6)-alkyl-C(═N—NO2)NR10R11, (C0-C6)-alkyl-C(O)OR10, (C0-C6)-alkyl-C(O)NR10R11, (C0-C6)-alkyl-C(O)NR10SO2R11, C(O)NR10—(C0-C6)-alkyl-heteroaryl, C(O)NR10—(C0-C6)-alkyl-aryl, S(O)2NR10—(C0-C6)-alkyl-aryl, S(O)2NR10—(C0-C6)-alkyl-heteroaryl, S(O)2NR10-alkyl, S(O)2—(C0-C6)-alkyl-aryl, S(O)2—(C0-C6)-alkyl-heteroaryl, (C0-C6)-alkyl-C(O)—NR11—CN, O—(C0-C6)-alkyl-C(O)NR10R11, S(O)x—(C0-C6)-alkyl-C(O)OR10, S(O)x—(C0-C6)-alkyl-C(O)NR10R11, (C0-C6)-alkyl-C(O)NR10—(C0-C6)-alkyl-NR10R11, (C0-C6)-alkyl-NR10—C(O)R10, (C0-C6)-alkyl-NR10—C(O)OR10, (C0-C6)-alkyl-NR10—C(O)—NR10R11, (C0-C6)-alkyl-NR10—S(O)yNR10R11, (C0-C6)-alkyl-NR10—S(O)yR11, O—(C0-C6)-alkyl-aryl and O—(C0-C6)-alkyl-heteroaryl, wherein each R9 group is substituted 0, 1 or 2 times; and the remaining R9 groups are hydrogen.

In one embodiment, in conjunction with any above or below embodiments, R9 is H.

In one embodiment, in conjunction with any above or below embodiments, R17 is selected from R9, alkenyl, alkynyl, bicycloalkyl, heterobicycloalkyl, spiroalkyl, spiroheteroalkyl, cycloalkyl fused aryl, heterocycloalkyl fused aryl, cycloalkyl fused heteroaryl, heterocycloalkyl fused heteroaryl or a bicyclic or tricyclic fused ring system, wherein at least one ring is partially saturated, and wherein each R17 group is substituted 0, 1 or 2 times and 0 or 1 R9 groups.

In one embodiment, in conjunction with any above or below embodiments, R30 is selected from alkyl and (C0-C6)-alkyl-aryl, wherein alkyl and aryl are optionally substituted 0, 1 or 2 times.

In one embodiment, in conjunction with any above or below embodiments, one R9 is sR50 in each occurrence is independently selected from hydrogen, alkyl, aryl, heteroaryl, C(O)R80, C(O)NR80R8, SO2R80 and SO2NR80R81, wherein alkyl, aryl, and heteroaryl are substituted 0, 1 or 2 times.

In one embodiment, in conjunction with any above or below embodiments, R80 and R81 in each occurrence are independently selected from hydrogen, alkyl, cycloalkyl, cycloalkylalkyl, heterocycloalkyl, fluoroalkyl, heterocycloalkylalkyl, haloalkyl, alkenyl, alkynyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl and aminoalkyl, wherein alkyl, cycloalkyl, cycloalkylalkyl, heterocycloalkyl, fluoroalkyl, heterocycloalkylalkyl, alkenyl, alkynyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl and aminoalkyl are optionally substituted 0, 1 or 2 times, or R80 and R81 when taken together with the nitrogen to which they are attached complete a 3- to 8-membered ring containing carbon atoms and optionally a heteroatom selected from O, S(O)x, NH, and N(alkyl) and which is optionally substituted 0, 1 or 2 times.

In one embodiment, in conjunction with any above or below embodiments, Lc is selected from a single bond or an acyclic, straight or branched, saturated or unsaturated hydrocarbon chain having 1 to 10 carbon atoms, optionally containing 1 to 3 groups independently selected from —S—, —O—, NR10—, —NR10CO—, —CONR10—, —S(O)x—, —SO2NR10—, —NR10SO2—, NR10SO2NR10—, —NR10CONR11—, —OC(O)NR10—, —NR10C(O)O—, which replace single carbon atoms, which in case that more than two carbon atoms are replaced are not adjacent, and wherein the hydrocarbon chain is optionally substituted one or more times;

In one embodiment, in conjunction with any above or below embodiments, Lc is absent.

In one embodiment, in conjunction with any above or below embodiments, Lc is selected from —CONH— and —NHCO—.

In one embodiment, in conjunction with any above or below embodiments, Ld is selected from a single bond or a straight or branched, saturated or unsaturated hydrocarbon chain having 1 to 10 carbon atoms, optionally containing 1, 2 or 3 groups independently selected from —O—, —NR10—, —S(O)x—, —NR10C(X1)—, —C(X1)NR10—, —SO2NR10—, —NR10 SO2—, —O—SO2—, —SO2—O—, —NR10SO2NR10—, —NR10C(X1)NR10—, —OC(X1)NR10—, —NR10C(X1)O—, —OC(X1)—, —C(X1)O—, -Q2-, —NR10-, -Q2-, -Q2-NR10—, C(X1)-Q2-, -Q2-C(X1)—, —O-Q2-, —S(O)x-Q2-, and -Q2-S(O)x— which replace single carbon atoms, which in case that more than two carbon atoms are replaced are not adjacent, and wherein the hydrocarbon chain is substituted 0, 1, 2 or 3 times;

In one embodiment, in conjunction with any above or below embodiments, Ld is selected from —CH2NHCO— and —CH2CONH—.

In one embodiment, in conjunction with any above or below embodiments, Ld is —CH2NHCO—.

In one embodiment, in conjunction with any above or below embodiments, Q1 is a 4-, 5-, 6-, 7- or 8-membered ring selected from cycloalkyl, heterocycloalkyl, bicycloalkyl, heterobicycloalkyl or a 5- or 6-membered ring selected from aryl and heteroaryl, wherein cycloalkyl, heterocycloalkyl, bicycloalkyl, heterobicycloalkyl, aryl and heteroaryl are substituted by 0, 1 or 2 R4 groups and optionally a substituent of Q1 is linked with Ld to complete a 3- to 8-membered ring containing carbon atoms and optionally heteroatoms selected from O, S(O)x, —NH, and —N(alkyl) wherein this new ring is optionally substituted one or more times.

In one embodiment, in conjunction with any above or below embodiments, Q1 is phenyl.

In one embodiment, in conjunction with any above or below embodiments, Q1 is pyridyl.

In one embodiment, in conjunction with any above or below embodiments, X1 is O.

In one embodiment, in conjunction with any above or below embodiments, Y is O.

In one embodiment, in conjunction with any above or below embodiments, Z1 is independently selected from C, S, S═O, PR10 and P—OR10.

Another aspect of the invention relates to a method of inhibiting a metalloprotease enzyme, comprising administering a compound selected from any of the above or below embodiments.

In another embodiment, in conjunction with any above or below embodiments, the metalloprotease is selected from MMP-3, MMP-8, and MMP-13.

In another embodiment, in conjunction with any above or below embodiments, the metalloprotease is MMP-13.

Another aspect of the invention relates to a method of treating a metalloprotease mediated disease, comprising administering to a subject in need of such treatment an effective amount of a compound selected from any of the above or below embodiments.

In another embodiment, in conjunction with any above or below embodiments, the disease is rheumatoid arthritis.

In another embodiment, in conjunction with any above or below embodiments, the disease is osteoarthritis.

In another embodiment, in conjunction with any above or below embodiments, the disease is inflammation.

In another embodiment, in conjunction with any above or below embodiments, the disease is atherosclerosis.

In another embodiment, in conjunction with any above or below embodiments, the disease is multiple sclerosis.

In another embodiment, in conjunction with any above or below embodiments, the disease is selected from: rheumatoid arthritis, osteoarthritis, abdominal aortic aneurysm, cancer (e.g. but not limited to melanoma, gastric carcinoma or non-small cell lung carcinoma), inflammation, atherosclerosis, chronic obstructive pulmonary disease, ocular diseases (e.g. but not limited to ocular inflammation, glaucoma, retinopathy of prematurity, macular degeneration with the wet type preferred and corneal neovascularization), neurologic diseases, psychiatric diseases, thrombosis, bacterial infection, Parkinson's disease, fatigue, tremor, diabetic retinopathy, vascular diseases of the retina, aging, dementia, cardiomyopathy, renal tubular impairment, diabetes, psychosis, dyskinesia, pigmentary abnormalities, deafness, inflammatory and fibrotic syndromes, intestinal bowel syndrome, allergies, Alzheimers disease, arterial plaque formation, oncology, periodontal, viral infection, stroke, atherosclerosis, cardiovascular disease, reperfusion injury, trauma, chemical exposure or oxidative damage to tissues, wound healing, hemorroid, skin beautifying, pain, inflammatory pain, bone pain and joint pain, acne, acute alcoholic hepatitis, acute inflammation, acute pancreatitis, acute respiratory distress syndrome, adult respiratory disease, airflow obstruction, airway hyperresponsiveness, alcoholic liver disease, allograft rejections, angiogenesis, angiogenic ocular disease, arthritis, asthma, atopic dermatitis, bronchiectasis, bronchiolitis, bronchiolitis obliterans, burn therapy, cardiac and renal reperfusion injury, celiac disease, cerebral and cardiac ischemia, CNS tumors, CNS vasculitis, colds, contusions, cor pulmonae, cough, Crohn's disease, chronic bronchitis, chronic inflammation, chronic pancreatitis, chronic sinusitis, crystal induced arthritis, cystic fibrosis, delayted type hypersensitivity reaction, duodenal ulcers, dyspnea, early transplantation rejection, emphysema, encephalitis, endotoxic shock, esophagitis, gastric ulcers, gingivitis, glomerulonephritis, glossitis, gout, graft vs. host reaction, gram negative sepsis, granulocytic ehrlichiosis, hepatitis viruses, herpes, herpes viruses, HIV, hypercapnea, hyperinflation, hyperoxia-induced inflammation, hypoxia, hypersensitivity, hypoxemia, inflammatory bowel disease, interstitial pneumonitis, ischemia reperfusion injury, kaposi's sarcoma associated virus, lupus, malaria, meningitis, multi-organ dysfunction, necrotizing enterocolitis, osteoporosis, chronic periodontitis, periodontitis, peritonitis associated with continous ambulatory peritoneal dialysis (CAPD), pre-term labor, polymyositis, post surgical trauma, pruritis, psoriasis, psoriatic arthritis, pulmatory fibrosis, pulmatory hypertension, renal reperfusion injury, respiratory viruses, restinosis, right ventricular hypertrophy, sarcoidosis, septic shock, small airway disease, sprains, strains, subarachnoid hemorrhage, surgical lung volume reduction, thrombosis, toxic shock syndrome, transplant reperfusion injury, traumatic brain injury, ulcerative colitis, vasculitis, ventilation-perfusion mismatching, and wheeze.

Another aspect of the invention relates to a pharmaceutical composition comprising:

A) an effective amount of a compound according to any of the above or below embodiments;

B) a pharmaceutically acceptable carrier; and

C) a drug, agent or therapeutic selected from: (a) a disease modifying antirheumatic drug; (b) a nonsteroidal anti-inflammatory drug; (c) a COX-2 selective inhibitor; (d) a COX-1 inhibitor; (e) an immunosuppressive; (f) a steroid; (g) a biological response modifier; (h) a viscosupplement; (i) a pain reducing drug; and (j) a small molecule inhibitor of pro-inflammatory cytokine production.

Another aspect of the invention relates to the use of a compound according to any of the above or below embodiments in the manufacture of a medicament for treating a metalloprotease mediated disease.

Another aspect of the invention relates to the use of a compound according to any of the above or below embodiments in conjunction with a a drug, agent or therapeutic selected from: (a) a disease modifying antirheumatic drug; (b) a nonsteroidal anti-inflammatory drug; (c) a COX-2 selective inhibitor; (d) a COX-1 inhibitor; (e) an immunosuppressive; (f) a steroid; (g) a biological response modifier; (h) a viscosupplement; (i) a pain reducing drug; and (j) a small molecule inhibitor of pro-inflammatory cytokine production, in the manufacture of a medicament for treating a metalloprotease mediated disease.

The terms “alkyl” or “alk”, as used herein alone or as part of another group, denote optionally substituted, straight and branched chain saturated hydrocarbon groups, preferably having 1 to 10 carbons in the normal chain, most preferably lower alkyl groups. Exemplary unsubstituted such groups include methyl, ethyl, propyl, isopropyl, n-butyl, t-butyl, isobutyl, pentyl, hexyl, isohexyl, heptyl, 4,4-dimethylpentyl, octyl, 2,2,4-trimethylpentyl, nonyl, decyl, undecyl, dodecyl and the like. Exemplary substituents may include, but are not limited to, one or more of the following groups: halo, alkoxy, alkylthio, alkenyl, alkynyl, aryl (e.g., to form a benzyl group), cycloalkyl, cycloalkenyl, hydroxy or protected hydroxy, carboxyl (—COOH), alkyloxycarbonyl, alkylcarbonyloxy, alkylcarbonyl, carbamoyl (NH2—CO—), substituted carbamoyl ((R10)(R11)N—CO— wherein R10 or R11 are as defined below, except that at least one of R10 or R11 is not hydrogen), amino, heterocyclo, mono- or dialkylamino, or thiol (—SH).

The terms “lower alk” or “lower alky” as used herein, denote such optionally substituted groups as described above for alkyl having 1 to 4 carbon atoms in the normal chain.

The term “alkoxy” denotes an alkyl group as described above bonded through an oxygen linkage (—O—).

The term “alkenyl”, as used herein alone or as part of another group, denotes optionally substituted, straight and branched chain hydrocarbon groups containing at least one carbon to carbon double bond in the chain, and preferably having 2 to 10 carbons in the normal chain. Exemplary unsubstituted such groups include ethenyl, propenyl, isobutenyl, butenyl, pentenyl, hexenyl, heptenyl, octenyl, nonenyl, decenyl, and the like. Exemplary substituents may include, but are not limited to, one or more of the following groups: halo, alkoxy, alkylthio, alkyl, alkynyl, aryl, cycloalkyl, cycloalkenyl, hydroxy or protected hydroxy, carboxyl (—COOH), alkyloxycarbonyl, alkylcarbonyloxy, alkylcarbonyl, carbamoyl (NH2—CO—), substituted carbamoyl ((R10)(R11)N—CO— wherein R10 or R11 are as defined below, except that at least one of R10 or R11 is not hydrogen), amino, heterocyclo, mono- or dialkylamino, or thiol (—SH).

The term “alkynyl”, as used herein alone or as part of another group, denotes optionally substituted, straight and branched chain hydrocarbon groups containing at least one carbon to carbon triple bond in the chain, and preferably having 2 to 10 carbons in the normal chain. Exemplary unsubstituted such groups include, but are not limited to, ethynyl, propynyl, butynyl, pentynyl, hexynyl, heptynyl, octynyl, nonynyl, decynyl, and the like. Exemplary substituents may include, but are not limited to, one or more of the following groups: halo, alkoxy, alkylthio, alkyl, alkenyl, aryl, cycloalkyl, cycloalkenyl, hydroxy or protected hydroxy, carboxyl (—COOH), alkyloxycarbonyl, alkylcarbonyloxy, alkylcarbonyl, carbamoyl (NH2—CO—), substituted carbamoyl ((R10)(R11)N—CO— wherein R10 or R11 are as defined below, except that at least one of R10 or R11 is not hydrogen), amino, heterocyclo, mono- or dialkylamino, or thiol (—SH).

The term “cycloalkyl”, as used herein alone or as part of another group, denotes optionally substituted, saturated cyclic hydrocarbon ring systems, desirably containing one ring with 3 to 9 carbons. Exemplary unsubstituted such groups include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, cyclodecyl, and cyclododecyl. Exemplary substituents include, but are not limited to, one or more alkyl groups as described above, or one or more groups described above as alkyl substituents.

The term “bicycloalkyl”, as used herein alone or as part of another group, denotes optionally substituted, saturated cyclic bridged hydrocarbon ring systems, desirably containing 2 or 3 rings and 3 to 9 carbons per ring. Exemplary unsubstituted such groups include, but are not limited to, adamantyl, bicyclo[2.2.2]octane, bicyclo[2.2.1]heptane and cubane. Exemplary substituents include, but are not limited to, one or more alkyl groups as described above, or one or more groups described above as alkyl substituents.

The term “spiroalkyl”, as used herein alone or as part of another group, denotes optionally substituted, saturated hydrocarbon ring systems, wherein two rings of 3 to 9 carbons per ring are bridged via one carbon atom. Exemplary unsubstituted such groups include, but are not limited to, spiro[3.5]nonane, spiro[4.5]decane or spiro[2.5]octane. Exemplary substituents include, but are not limited to, one or more alkyl groups as described above, or one or more groups described above as alkyl substituents.

The term “spiroheteroalkyl”, as used herein alone or as part of another group, denotes optionally substituted, saturated hydrocarbon ring systems, wherein two rings of 3 to 9 carbons per ring are bridged via one carbon atom and at least one carbon atom is replaced by a heteroatom independently selected from N, O and S. The nitrogen and sulfur heteroatoms may optionally be oxidized. Exemplary unsubstituted such groups include, but are not limited to, 1,3-diaza-spiro[4.5]decane-2,4-dione. Exemplary substituents include, but are not limited to, one or more alkyl groups as described above, or one or more groups described above as alkyl substituents.

The terms “ar” or “aryl”, as used herein alone or as part of another group, denote optionally substituted, homocyclic aromatic groups, preferably containing 1 or 2 rings and 6 to 12 ring carbons. Exemplary unsubstituted such groups include, but are not limited to, phenyl, biphenyl, and naphthyl. Exemplary substituents include, but are not limited to, one or more nitro groups, alkyl groups as described above or groups described above as alkyl substituents.

The term “heterocycle” or “heterocyclic system” denotes a heterocyclyl, heterocyclenyl, or heteroaryl group as described herein, which contains carbon atoms and from 1 to 4 heteroatoms independently selected from N, O and S and including any bicyclic or tricyclic group in which any of the above-defined heterocyclic rings is fused to one or more heterocycle, aryl or cycloalkyl groups. The nitrogen and sulfur heteroatoms may optionally be oxidized. The heterocyclic ring may be attached to its pendant group at any heteroatom or carbon atom which results in a stable structure. The heterocyclic rings described herein may be substituted on carbon or on a nitrogen atom.

Examples of heterocycles include, but are not limited to, 1H-indazole, 2-pyrrolidonyl, 2H,6H-1,5,2-dithiazinyl, 2H-pyrrolyl, 3H-indolyl, 4-piperidonyl, 4aH-carbazole, 4H-quinolizinyl, 6H-1,2,5-thiadiazinyl, acridinyl, azocinyl, benzimidazolyl, benzofuranyl, benzothiofuranyl, benzothiophenyl, benzoxazolinyl, benzoxazolyl, benzthiazolyl, benztriazolyl, benztetrazolyl, benzisoxazolyl, benzisothiazolyl, benzimidazalonyl, carbazolyl, 4aH-carbazolyl, b-carbolinyl, chromanyl, chromenyl, cinnolinyl, decahydroquinolinyl, 2H,6H-1,5,2-dithiazinyl, dihydrofuro[2,3-b]tetrahydrofuran, furanyl, furazanyl, imidazolidinyl, imidazolinyl, imidazolyl, 1H-indazolyl, indolenyl, indolinyl, indolizinyl, indolyl, isatinoyl, isobenzofuranyl, isochromanyl, isoindazolyl, isoindolinyl, isoindolyl, isoquinolinyl, isothiazolyl, isoxazolyl, morpholinyl, naphthyridinyl, octahydroisoquinolinyl, oxadiazolyl, 1,2,3-oxadiazolyl, 1,2,4-oxadiazolyl, 1,2,5-oxadiazolyl, 1,3,4-oxadiazolyl, oxazolidinyl, oxazolyl, oxazolidinylperimidinyl, oxindolyl, phenanthridinyl, phenanthrolinyl, phenarsazinyl, phenazinyl, phenothiazinyl, phenoxathiinyl, phenoxazinyl, phthalazinyl, piperazinyl, piperidinyl, pteridinyl, piperidonyl, 4-piperidonyl, pteridinyl, purinyl, pyranyl, pyrazinyl, pyrazolidinyl, pyrazolinyl, pyrazolyl, pyridazinyl, pyridooxazole, pyridoimidazole, pyridothiazole, pyridinyl, pyridyl, pyrimidinyl, pyrrolidinyl, pyrrolinyl, pyrrolyl, quinazolinyl, quinolinyl, 4H-quinolizinyl, quinoxalinyl, quinuclidinyl, carbolinyl, tetrahydrofuranyl, tetrahydroisoquinolinyl, tetrahydroquinolinyl, tetrazolyl, 6H-1,2,5-thiadiazinyl, 1,2,3-thiadiazolyl, 1,2,4-thiadiazolyl, 1,2,5-thiadiazolyl, 1,3,4-thiadiazolyl, thianthrenyl, thiazolyl, thienyl, thienothiazolyl, thienooxazolyl, thienoimidazolyl, thiophenyl, triazinyl, 1,2,3-triazolyl, 1,2,4-triazolyl, 1,2,5-triazolyl, 1,3,4-triazolyl, xanthenyl.

Further examples of heterocycles include, but not are not limited to, “heterobicycloalkyl” groups such as 7-oxa-bicyclo[2.2.1 ]heptane, 7-aza-bicyclo[2.2.1]heptane, and 1-aza-bicyclo[2.2.2]octane. “Heterocyclenyl” denotes a non-aromatic monocyclic or multicyclic hydrocarbon ring system of about 3 to about 10 atoms, desirably about 4 to about 8 atoms, in which one or more of the carbon atoms in the ring system is/are hetero element(s) other than carbon, for example nitrogen, oxygen or sulfur atoms, and which contains at least one carbon-carbon double bond or carbon-nitrogen double bond. Ring sizes of rings of the ring system may include 5 to 6 ring atoms. The designation of the aza, oxa or thia as a prefix before heterocyclenyl define that at least a nitrogen, oxygen or sulfur atom is present respectively as a ring atom. The heterocyclenyl may be optionally substituted by one or more substituents as defined herein. The nitrogen or sulphur atom of the heterocyclenyl may also be optionally oxidized to the corresponding N-oxide, S-oxide or S,S-dioxide. “Heterocyclenyl” as used herein includes by way of example and not limitation those described in Paquette, Leo A.; “Principles of Modem Heterocyclic Chemistry” (W. A. Benjamin, New York, 1968), particularly Chapters 1, 3, 4, 6, 7, and 9; “The Chemistry of Heterocyclic Compounds, A series of Monographs” (John Wiley & Sons, New York, 1950 to present), in particular Volumes 13, 14, 16, 19, and 28; and “J. Am. Chem. Soc.”, 82:5566 (1960), the contents all of which are incorporated by reference herein. Exemplary monocyclic azaheterocyclenyl groups include, but are not limited to, 1,2,3,4-tetrahydrohydropyridine, 1,2-dihydropyridyl, 1,4-dihydropyridyl, 1,2,3,6-tetrahydropyridine, 1,4,5,6-tetrahydropyrimidine, 2-pyrrolinyl, 3-pyrrolinyl, 2-imidazolinyl, 2-pyrazolinyl, and the like. Exemplary oxaheterocyclenyl groups include, but are not limited to, 3,4-dihydro-2H-pyran, dihydrofuranyl, and fluorodihydrofuranyl. An exemplary multicyclic oxaheterocyclenyl group is 7-oxabicyclo[2.2.1]heptenyl.

“Heterocyclyl,”or “heterocycloalkyl,” denotes a non-aromatic saturated monocyclic or multicyclic ring system of about 3 to about 10 carbon atoms, desirably 4 to 8 carbon atoms, in which one or more of the carbon atoms in the ring system is/are hetero element(s) other than carbon, for example nitrogen, oxygen or sulfur. Ring sizes of rings of the ring system may include 5 to 6 ring atoms. The designation of the aza, oxa or thia as a prefix before heterocyclyl define that at least a nitrogen, oxygen or sulfur atom is present respectively as a ring atom. The heterocyclyl may be optionally substituted by one or more substituents which may be the same or different, and are as defined herein. The nitrogen or sulphur atom of the heterocyclyl may also be optionally oxidized to the corresponding N-oxide, S-oxide or S,S-dioxide.

“Heterocyclyl” as used herein includes by way of example and not limitation those described in Paquette, Leo A.; “Principles of Modern Heterocyclic Chemistry” (W. A. Benjamin, New York, 1968), particularly Chapters 1, 3, 4, 6, 7, and 9; “The Chemistry of Heterocyclic Compounds, A series of Monographs” (John Wiley & Sons, New York, 1950 to present), in particular Volumes 13, 14, 16, 19, and 28; and “J. Am. Chem. Soc.”, 82:5566 (1960). Exemplary monocyclic heterocyclyl rings include, but are not limited to, piperidyl, pyrrolidinyl, piperazinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, 1,3-dioxolanyl, 1,4-dioxanyl, tetrahydrofuranyl, tetrahydrothiophenyl, tetrahydrothiopyranyl, and the like.

“Heteroaryl” denotes an aromatic monocyclic or multicyclic ring system of about 5 to about 10 atoms, in which one or more of the atoms in the ring system is/are hetero element(s) other than carbon, for example nitrogen, oxygen or sulfur. Ring sizes of rings of the ring system include 5 to 6 ring atoms. The “heteroaryl” may also be substituted by one or more substituents which may be the same or different, and are as defined herein. The designation of the aza, oxa or thia as a prefix before heteroaryl define that at least a nitrogen, oxygen or sulfur atom is present respectively as a ring atom. A nitrogen atom of a heteroaryl may be optionally oxidized to the corresponding N-oxide. Heteroaryl as used herein includes by way of example and not limitation those described in Paquette, Leo A.; “Principles of Modem Heterocyclic Chemistry” (W. A. Benjamin, New York, 1968), particularly Chapters 1, 3, 4, 6, 7, and 9; “The Chemistry of Heterocyclic. Compounds, A series of Monographs” (John Wiley & Sons, New York, 1950 to present), in particular Volumes 13, 14, 16, 19, and 28; and “J. Am. Chem. Soc. ”, 82:5566 (1960). Exemplary heteroaryl and substituted heteroaryl groups include, but are not limited to, pyrazinyl, thienyl, isothiazolyl, oxazolyl, pyrazolyl, furazanyl, pyrrolyl, 1,2,4-thiadiazolyl, pyridazinyl, quinoxalinyl, phthalazinyl, imidazo[1,2-a]pyridine, imidazo[2,1-b]thiazolyl, benzofurazanyl, azaindolyl, benzimidazolyl, benzothienyl, thienopyridyl, thienopyrimidyl, pyrrolopyridyl, imidazopyridyl, benzoazaindole, 1,2,3-triazinyl, 1,2,4-triazinyl, 1,3,5-triazinyl, benzthiazolyl, dioxolyl, furanyl, imidazolyl, indolyl, indolizinyl, isoxazolyl, isoquinolinyl, isothiazolyl, oxadiazolyl, oxazinyl, oxiranyl, piperazinyl, piperidinyl, pyranyl, pyrazinyl, pyridazinyl, pyrazolyl, pyridyl, pyrimidinyl, pyrrolyl, pyrrolidinyl, quinazolinyl, quinolinyl, tetrazinyl, tetrazolyl, 1,3,4-thiadiazolyl, 1,2,3-thiadiazolyl, 1,2,4-thiadiazolyl, 1,2,5-thiadiazolyl, thiatriazolyl, thiazinyl, thiazolyl, thienyl, 5-thioxo-1,2,4-diazolyl, thiomorpholino, thiophenyl, thiopyranyl, triazolyl and triazolonyl.

The phrase “fused” means, that the group, mentioned before “fused” is connected via two adjacent atoms to the ring system mentioned after “fused” to form a bicyclic system. For example, “heterocycloalkyl fused aryl” includes, but is not limited to, 2,3-dihydro-benzo[1,4]dioxine, 4H-benzo[1,4]oxazin-3-one, 3H-Benzooxazol-2-one and 3,4-dihydro-2H-benzo[f][1,4]oxazepin-5-one.

The term “amino” denotes the radical —NH2 wherein one or both of the hydrogen atoms may be replaced by an optionally substituted hydrocarbon group. Exemplary amino groups include, but are not limited to, n-butylamino, tert-butylamino, methylpropylamino and ethyldimethylamino.

The term “cycloalkylalkyl” denotes a cycloalkyl-alkyl group wherein a cycloalkyl as described above is bonded through an alkyl, as defined above. Cycloalkylalkyl groups may contain a lower alkyl moiety. Exemplary cycloalkylalkyl groups include, but are not limited to, cyclopropylmethyl, cyclopentylmethyl, cyclohexylmethyl, cyclopropylethyl, cyclopentylethyl, cyclohexylpropyl, cyclopropylpropyl, cyclopentylpropyl, and cyclohexylpropyl.

The term “arylalkyl” denotes an aryl group as described above bonded through an alkyl, as defined above.

The term “heteroarylalkyl” denotes a heteroaryl group as described above bonded through an alkyl, as defined above.

The term “heterocyclylalkyl,” or “heterocycloalkylalkyl,” denotes a heterocyclyl group as described above bonded through an alkyl, as defined above.

The terms “halogen”, “halo”, or “hal”, as used herein alone or as part of another group, denote chlorine, bromine, fluorine, and iodine.

The term “haloalkyl” denotes a halo group as described above bonded though an alkyl, as defined above. Fluoroalkyl is an exemplary group.

The term “aminoalkyl” denotes an amino group as defined above bonded through an alkyl, as defined above.

The phrase “bicyclic fused ring system wherein at least one ring is partially saturated” denotes an 8- to 13-membered fused bicyclic ring group in which at least one of the rings is non-aromatic. The ring group has carbon atoms and optionally 1-4 heteroatoms independently selected from N, O and S. The nitrogen and sulfur heteroatoms may optionally be oxidized. Illustrative examples include, but are not limited to, indanyl, tetrahydronaphthyl, tetrahydroquinolyl and benzocycloheptyl.

The phrase “tricyclic fused ring system wherein at least one ring is partially saturated” denotes a 9- to 1 8-membered fused tricyclic ring group in which at least one of the rings is non-aromatic. The ring group has carbon atoms and optionally 1-7 heteroatoms independently selected from N, O and S. The nitrogen and sulfur heteroatoms may optionally be oxidized. Illustrative examples include, but are not limited to, fluorene, 10,11-dihydro-5H-dibenzo[a,d]cycloheptene and 2,2a,7,7a-tetrahydro-1H-cyclobuta[a]indene.

The phrase “cyclic” denotes to a saturated, partially unsaturated or unsaturated ring group with one ring. The ring group has carbon atoms and optionally 1-10 heteroatoms independently selected from N, O and S. The nitrogen and sulfur heteroatoms may optionally be oxidized. Illustrative examples include, but are not limited to, cyclobutane, cyclohexene, morpholine, tetrahydrofurane, benzene, thiophene, imidazole.

The phrase “biyclic” denotes to a saturated, partially unsaturated or unsaturated ring group with two ring. The ring group has carbon atoms and optionally 1-10 heteroatoms independently selected from N, O and S. The nitrogen and sulfur heteroatoms may optionally be oxidized. The rings may be annulated or otherwise connected, e.g. via a spiro connectivity. Illustrative examples include, but are not limited to, indane, tetrahydronaphthalin, tetrahydroquinoline, benzocycloheptane, and 1,3-diaza-spiro[4.5]decane-2,4-dione.

The phrase “multicyclic” denotes to a saturated, partially unsaturated or unsaturated ring group with at least three rings. The ring group has carbon atoms and optionally 1-10 heteroatoms independently selected from N, O and S. The nitrogen and sulfur heteroatoms may optionally be oxidized. The rings may be annulated or otherwise connected, e.g. via a spiro connectivity. Illustrative examples include, but are not limited to, fluorene, adamantyl, bicyclo[2.2.2]octane, bicyclo[2.2.1]heptane, cubane, 10,11-dihydro-5H-dibenzo[a,d]cycloheptene, 2,2a,7,7a-tetrahydro-1H-cyclobuta[a]indene, 5,6,7,8-tetrahydro-benzo[4,5]thieno[2,3-d]pyrimidine, 11-oxa-3,5-diaza-tricyclo[6.2.1.02,7]undeca-2(7),3,5-triene, 3,5-diaza-tricyclo[6.2.2.02,7]dodeca-2(7),3-dien-6-one.

The term “pharmaceutically acceptable salts” refers to derivatives of the disclosed compounds wherein the parent compound is modified by making acid or base salts thereof. Examples of pharmaceutically acceptable salts include, but are not limited to, mineral or organic acid salts of basic residues such as amines; alkali or organic salts of acidic residues such as carboxylic acids; and the like. Examples therefore may be, but are not limited to, sodium, potassium, choline, lysine, arginine or N-methyl-glucamine salts, and the like.

The pharmaceutically acceptable salts include the conventional non-toxic salts or the quaternary ammonium salts of the parent compound formed, for example, from non-toxic inorganic or organic acids. For example, such conventional non-toxic salts include those derived from inorganic acids such as, but not limited to, hydrochloric, hydrobromic, sulfuric, sulfamic, phosphoric, nitric and the like; and the salts prepared from organic acids such as, but not limited to, acetic, propionic, succinic, glycolic, stearic, lactic, malic, tartaric, citric, ascorbic, pamoic, maleic, hydroxymaleic, phenylacetic, glutamic, benzoic, salicylic, sulfanilic, 2-acetoxybenzoic, fumaric, toluenesulfonic, methanesulfonic, ethane disulfonic, oxalic, isethionic, and the like.

The pharmaceutically acceptable salts of the present invention can be synthesized from the parent compound which contains a basic or acidic moiety by conventional chemical methods. Generally, such salts can be prepared by reacting the free acid or base forms of these compounds with a stoichiometric amount of the appropriate base or acid in water or in an organic solvent, or in a mixture of the two. Organic solvents include, but are not limited to, nonaqueous media like ethers, ethyl acetate, ethanol, isopropanol, or acetonitrile. Lists of suitable salts are found in Remington's Pharmaceutical Sciences, 18th ed., Mack Publishing Company, Easton, Pa., 1990, p. 1445, the disclosure of which is hereby incorporated by reference.

The phrase “pharmaceutically acceptable” denotes those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication commensurate with a reasonable benefit/risk ratio.

The phrase “pharmaceutically acceptable carrier” denotes media generally accepted in the art for the delivery of biologically active agents to mammals, e.g., humans. Such carriers are generally formulated according to a number of factors well within the purview of those of ordinary skill in the art to determine and account for. These include, without limitation: the type and nature of the active agent being formulated; the subject to which the agent-containing composition is to be administered; the intended route of administration of the composition; and, the therapeutic indication being targeted. Pharmaceutically acceptable carriers include both aqueous and non-aqueous liquid media, as well as a variety of solid and semi-solid dosage forms. Such carriers can include a number of different ingredients and additives in addition to the active agent, such additional ingredients being included in the formulation for a variety of reasons, e.g., stabilization of the active agent, well known to those of ordinary skill in the art. Non-limiting examples of a pharmaceutically acceptable carrier are hyaluronic acid and salts thereof, and microspheres (including, but not limited to poly(D,L)-lactide-co-glycolic acid copolymer (PLGA), poly(L-lactic acid) (PLA), poly(caprolactone (PCL) and bovine serum albumin (BSA)). Descriptions of suitable pharmaceutically acceptable carriers, and factors involved in their selection, are found in a variety of readily available sources, e.g., Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., 1985, the contents of which are incorporated herein by reference.

Pharmaceutically acceptable carriers particularly suitable for use in conjunction with tablets include, for example, inert diluents, such as celluloses, calcium or sodium carbonate, lactose, calcium or sodium phosphate; disintegrating agents, such as croscarmellose sodium, cross-linked povidone, maize starch, or alginic acid; binding agents, such as povidone, starch, gelatin or acacia; and lubricating agents, such as magnesium stearate, stearic acid or talc. Tablets may be uncoated or may be coated by known techniques including microencapsulation to delay disintegration and adsorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monostearate or glyceryl distearate alone or with a wax may be employed.

Formulations for oral use may be also presented as hard gelatin capsules where the active ingredient is mixed with an inert solid diluent, for example celluloses, lactose, calcium phosphate or kaolin, or as soft gelatin capsules wherein the active ingredient is mixed with non-aqueous or oil medium, such as glycerin, propylene glycol, polyethylene glycol, peanut oil, liquid paraffin or olive oil.

The compositions of the invention may also be formulated as suspensions including a compound of the present invention in admixture with at least one pharmaceutically acceptable excipient suitable for the manufacture of a suspension. In yet another embodiment, pharmaceutical compositions of the invention may be formulated as dispersible powders and granules suitable for preparation of a suspension by the addition of suitable excipients.

Carriers suitable for use in connection with suspensions include suspending agents, such as sodium carboxymethylcellulose, methylcellulose, hydroxypropyl methylcelluose, sodium alginate, polyvinylpyrrolidone, gum tragacanth, gum acacia, dispersing or wetting agents such as a naturally occurring phosphatide (e.g., lecithin), a condensation product of an alkylene oxide with a fatty acid (e.g., polyoxyethylene stearate), a condensation product of ethylene oxide with a long chain aliphatic alcohol (e.g., heptadecaethyleneoxycethanol), a condensation product of ethylene oxide with a partial ester derived from a fatty acid and a hexitol anhydride (e.g., polyoxyethylene sorbitan monooleate); and thickening agents, such as carbomer, beeswax, hard paraffin or cetyl alcohol. The suspensions may also contain one or more preservatives such as acetic acid, methyl and/or n-propyl p-hydroxy-benzoate; one or more coloring agents; one or more flavoring agents; and one or more sweetening agents such as sucrose or saccharin.

Cyclodextrins may be added as aqueous solubility enhancers. Preferred cyclodextrins include hydroxypropyl, hydroxyethyl, glucosyl, maltosyl and maltotriosyl derivatives of α-, β-, and γ-cyclodextrin. The amount of solubility enhancer employed will depend on the amount of the compound of the present invention in the composition.

The term “formulation” denotes a product comprising the active ingredient(s) and the inert ingredient(s) that make up the carrier, as well as any product which results, directly or indirectly, from combination, complexation or aggregation of any two or more of the ingredients, or from dissociation of one or more of the ingredients, or from other types of reactions or interactions of one or more of the ingredients. Accordingly, the pharmaceutical formulations of the present invention encompass any composition made by admixing a compound of the present invention and a pharmaceutical carrier.

The term “N-oxide” denotes compounds that can be obtained in a known manner by reacting a compound of the present invention including a nitrogen atom (such as in a pyridyl group) with hydrogen peroxide or a peracid, such as 3-chloroperoxy-benzoic acid, in an inert solvent, such as dichloromethane, at a temperature between about −10° C. to 80° C., desirably about 0° C.

The term “polymorph” denotes a form of a chemical compound in a particular crystalline arrangement. Certain polymorphs may exhibit enhanced thermodynamic stability and may be more suitable than other polymorphic forms for inclusion in pharmaceutical formulations.

The compounds of the invention can contain one or more chiral centers and/or double bonds and, therefore, exist as stereoisomers, such as double-bond isomers (i.e., geometric isomers), enantiomers, or diastereomers. According to the invention, the chemical structures depicted herein, and therefore the compounds of the invention, encompass all of the corresponding enantiomers and stereoisomers, that is, both the stereomerically pure form (e.g., geometrically pure, enantiomerically pure, or diastereomerically pure) and enantiomeric and stereoisomeric mixtures.

The term “racemic mixture” denotes a mixture that is about 50% of one enantiomer and about 50% of the corresponding enantiomer relative to all chiral centers in the molecule. Thus, the invention encompasses all enantiomerically-pure, enantiomerically-enriched, and racemic mixtures of compounds of Formula (I).

Enantiomeric and stereoisomeric mixtures of compounds of the invention can be resolved into their component enantiomers or stereoisomers by well-known methods. Examples include, but are not limited to, the formation of chiral salts and the use of chiral or high performance liquid chromatography “HPLC” and the formation and crystallization of chiral salts. See, e.g., Jacques, J., et al., Enantiomers, Racemates and Resolutions (Wiley-Interscience, New York, 1981); Wilen, S. H., et al., Tetrahedron 33:2725 (1977); Eliel, E. L., Stereochemistry of Carbon Compounds (McGraw-Hill, NY, 1962); Wilen, S. H., Tables of Resolving Agents and Optical Resolutions p. 268 (E. L. Eliel, Ed., Univ. of Notre Dame Press, Notre Dame, Ind., 1972); Stereochemistry of Organic Compounds, Ernest L. Eliel, Samuel H. Wilen and Lewis N. Manda (1994 John Wiley & Sons, Inc.), and Stereoselective Synthesis A Practical Approach, Mihaly Nogradi (1995 VCH Publishers, Inc., NY, N.Y.). Enantiomers and stereoisomers can also be obtained from stereomerically- or enantiomerically-pure intermediates, reagents, and catalysts by well-known asymmetric synthetic methods.

“Substituted” is intended to indicate that one or more hydrogens on the atom indicated in the expression using “substituted” is replaced with a selection from the indicated group(s), provided that the indicated atom's normal valency is not exceeded, and that the substitution results in a stable compound. When a substituent is keto (i.e., ═O) group, then two hydrogens on the atom are replaced. Furthermore two hydrogens on the atom can be relaced to form a thiocarbonyl (i.e., ═S) or ═N—NO2, ═N—CN, ═N—H, ═N—(C1-C4)alkyl, ═N—OH, ═N—O(C1-C4)alkyl, ═N—CO(C1-C4)alkyl, and ═N—SO2(C1-C4)alkyl.

Unless moieties of a compound of the present invention are defined as being unsubstituted, the moieties of the compound may be substituted. In addition to any substituents provided above, the moieties of the compounds of the present invention may be optionally substituted with one or more groups independently selected from:

  • B(OH)2;
  • B(O—(C1-C)alkyl)2;
  • C1-C4 alkyl;
  • C2-C4 alkenyl;
  • C2-C4 alkynyl;
  • CF3;
  • halo;
  • OH;
  • O—(C1-C4 alkyl);
  • OCH2F;
  • OCHF2;
  • OCF3;
  • ONO2;
  • OC(O)—(C1-C4 alkyl);
  • OC(O)—(C1-C4 alkyl);
  • OC(O)NH—(C1-C4 alkyl);
  • OC(O)N(C1-C4 alkyl)2;
  • OC(S)NH—(C1-C4 alkyl);
  • OC(S)N(C1-C4 alkyl)2;
  • SH;
  • S—(C1-C4 alkyl);
  • S(O)—(C1-C4 alkyl);
  • S(O)2—(C1-C4 alkyl);
  • SC(O)—(C1-C4 alkyl);
  • SC(O)O—(C1-C4 alkyl);
  • NH2;
  • N(H)—(C1-C4 alkyl);
  • N(C1-C4 alkyl)2;
  • N(H)C(O)—(C1-C4 alkyl);
  • N(CH3)C(O)—(C1-C4 alkyl);
  • N(H)C(O)—CF3;
  • N(CH3)C(O)—CF3;
  • N(H)C(S)—(C1-C4 alkyl);
  • N(CH3)C(S)—(C1-C4 alkyl);
  • N(H)S(O)2—(C1-C4 alkyl);
  • N(H)C(O)NH2;
  • N(H)C(O)NH—(C1-C4 alkyl);
  • N(CH3)C(O)NH—(C1-C4 alkyl);
  • N(H)C(O)N(C1-C4 alkyl)2;
  • N(CH3)C(O)N(C1-C4 alkyl)2;
  • N(H)S(O)2NH2);
  • N(H)S(O)2NH—(C1-C4 alkyl);
  • N(CH3)S(O)2NH—(C1-C4 alkyl);
  • N(H)S(O)2N(C1-C4 alkyl)2;
  • N(CH3)S(O)2N(C1-C4 alkyl)2;
  • N(H)C(O)O—(C1-C4 alkyl);
  • N(CH3)C(O)O—(C1-C4 alkyl);
  • N(H)S(O)2O—(C1-C4 alkyl);
  • N(CH3)S(O)2O—(C1-C4 alkyl);
  • N(CH3)C(S)NH—(C1-C4 alkyl);
  • N(CH3)C(S)N(C1-C4 alkyl)2;
  • N(CH3)C(S)O—(C1-C4 alkyl);
  • N(H)C(S)NH2;
  • NO2;
  • CO2H;
  • CO2—(C1-C4 alkyl);
  • C(O)N(H)OH;
  • C(O)N(CH3)OH:
  • C(O)N(CH3)OH;
  • C(O)N(CH3)O—(C1-C4 alkyl);
  • C(O)N(H)—(C1-C4 alkyl);
  • C(O)N(C1-C4 alkyl)2;
  • C(S)N(H)—(C1-C4 alkyl);
  • C(S)N(C1-C4 alkyl)2;
  • C(NH)N(H)—(C1-C4 alkyl);
  • C(NH)N(C1-C4 alkyl)2;
  • C(NCH3)N(H)—(C1-C4 alkyl);
  • C(NCH3)N(C1-C4 alkyl)2;
  • C(O)—(C1-C4 alkyl);
  • C(NH)—(C1-C4 alkyl);
  • C(NCH3)—(C1-C4 alkyl);
  • C(NOH)—(C1-C4 alkyl);
  • C(NOCH3)—(C1-C4 alkyl);
  • CN;
  • CHO;
  • CH2OH;
  • CH2O—(C1-C4 alkyl);
  • CH2NH2;
  • CH2N(H)—(C1-C4 alkyl);
  • CH2N(C1-C4 alkyl)2;
  • aryl;
  • heteroaryl;
  • cycloalkyl; and
  • heterocyclyl.

In some cases, a ring substituent may be shown as being connected to the ring by a bond extending from the center of the ring. The number of such substituents present on a ring is indicated in subscript by a number. Moreover, the substituent may be present on any available ring atom, the available ring atom being any ring atom which bears a hydrogen which the ring substituent may replace. For illustrative purposes, if variable RX were defined as being:

this would indicate a cyclohexyl ring bearing five RX substituents. The RX substituents may be bonded to any available ring atom. For example, among the configurations encompassed by this are configurations such as:

These configurations are illustrative and are not meant to limit the scope of the invention in any way.

When cyclic ring systems are illustrated with cyles or fragment of cycles in the formula, it is meant that the bridge atom connecting the cyclic ring systems with an the substituent (e.g. another ring) can be a carbon or nitrogen atom. For illustrative purposes, if the fragment QX were defined as being a ring, wherein two adjacent atoms are substituted to form an additional 6-membered ring:

this would indicate that e.g. the following structures are possible:

Biological Activity

The inhibiting activity towards different metalloproteases of the heterocyclic metalloprotease inhibiting compounds of the present invention may be measured using any suitable assay known in the art. A standard in vitro assay for measuring the metalloprotease inhibiting activity is described in Examples 1700 to 1704. The heterocyclic metalloprotease inhibiting compounds show activity towards MMP-3, MMP-8 and/or MMP-13.

Some heterocyclic metalloprotease inhibiting compounds of the invention have an MMP-13 inhibition activity (IC50 MMP-13) ranging from below 0.1 nM to about 20 μM, and typically, from about 1 nM to about 1 μM. Heterocyclic metalloprotease inhibiting compounds of the invention desirably have an MMP inhibition activity ranging from below 0.2 nM to about 20 nM. Table 1 lists typical examples of heterocyclic metalloprotease inhibiting compounds of the invention that have an MMP-13 activity lower than 100 nM (Group A) and from 100 nM to 20 μM (Group B).

TABLE 1 Summary of MMP-13 Activity for Compounds Group Ex. # A 1/2, 1/4, 1/6, 1/7, 1/9, 1/10, 1/11, 1/12, 1/15, 1/16, 1/17, 1/18, 1/19, 1/25, 1/26, 1/29, 1/32, 1/33, 3 B 1, 1/1, 1/5, 1/8, 1/13, 1/23, 1/24, 1/27, 1/30, 1/34

Some heterocyclic metalloprotease inhibiting compounds of the invention have an MMP-8 inhibition activity (IC50 MMP-8) ranging from below 100 nM to about 20 μM, and typically, from about 100 nM to about 2 μM. Heterocyclic metalloprotease inhibiting compounds of the invention desirably have an MMP inhibition activity ranging below 100 nM. Table 2 lists typical examples of heterocyclic metalloprotease inhibiting compounds of the invention that have an MMP-8 activity lower than 250 nM (Group A) and from 250 nM to 20 μM (Group B).

TABLE 2 Summary of MMP-8 Activity for Compounds Group Ex. # A 1/4, 1/9, 1/12, 1/17, 1/32, 3 B 1/6, 1/7, 1/8, 1/15, 1/16, 1/26

Some heterocyclic metalloprotease inhibiting compounds of the invention have an MMP-3 inhibition activity (IC50 MMP-3) ranging from below 50 nM to about 20 μM, and typically, from about 10 nM to about 2 μM. Heterocyclic metalloprotease inhibiting compounds of the invention desirably have an MMP inhibition activity ranging below 100 nM. Table 2 lists typical examples of heterocyclic metalloprotease inhibiting compounds of the invention that have an MMP-3 activity lower than 250 nM (Group A) and from 250 nM to 20 μM (Group B).

TABLE 3 Summary of MMP-3 Activity for Compounds Group Ex. # A 1/4, 1/9, 1/32 B 1/29

The synthesis of metalloprotease inhibiting compounds of the invention and their biological activity assay are described in the following examples which are not intended to be limiting in any way.

Schemes

Provided below are schemes according to which compounds of the present invention may be prepared. Exemplary amine building blocks of the following structure

can be obtained similar as described e.g. in WO2006/083454 and WO2006/128184, with the following representative amine building blocks:

In some embodiments the compounds of Formula (I) are synthesized by the general methods shown in Scheme 1 to Scheme 4.

route A

An carbonic acid and amino substituted compound (e.g. 4-amino-nicotinic acid) is condensed (e.g. EtOH/reflux) with chloro-oxo-acetic acid ethyl ester as previously described e.g. in WO2005/105760 in pyridine to give an oxazine ethyl ester (Scheme 1). This intermediate is then converted into the corresponding pyrimidine derivative using a suitable reagent (e.g. NH4OAc, HOAc, EtOH/80° C.). For example, when ring Q is a pyridine ring. the compound can be obtained according this route A.

route B

An ester and amino substituted compound (e.g. 2-amino-benzoic acid ethyl ester) is condensed (e.g. 4N HCl, dioxane/50° C.) with ethyl cyanoformate as previously described e.g. in WO2005/105760, to give a 1,3-pyrimidine-4-one ethyl ester (Scheme 1).

route C

An carboxamide and amino substituted compound (e.g. 2-amino-benzamide) is condensed with an suitable reagent (e.g oxalic acid diethyl ester or acetic acid anhydride as describend in DD272079A1 or chloro-oxo-acetic acid ethyl ester as described in J. Med. Chem. 1979, 22(5), 505-510) to give a 1,3-pyrimidine-4-one ethyl ester (Scheme 1).

In case, where Q in Scheme 1 is a thiophene moiety, these derivatives can be synthesized via the Gewald reaction (J. prakt. Chem. 1973, 315, 39-43, Monatsh. Chem. 2001,132, 279-293).

Representative procedures are provided in the application U.S. 60/860,195 (Nov. 20, 2006).

Saponification (e.g. aqueous LiOH) of the 1,3-pyrimidine-4-one derivative of Scheme 1 above gives the corresponding bicyclic carboxylic acid (Scheme 2). Activated acid coupling (e.g. EDCI/HOAt) with R1R2NH (e.g. 6-aminomethyl-4H-benzo[1,4]oxazin-3-one) in a suitable solvent gives the desired amide. The saponification/coupling step can be combined by stirring the ester with the free amine at elevated temperature (e.g. 200° C., 15 min) under microwave irradiation.

In compounds, where the bridge atom is a nitrogen atom, the following procedure can be applied (Scheme 3).

For example, N-(pyrazol-3-yl) acetamide acetate can be cyclizised with carbonic acid diethyl ester to 2-methylpyrazolo[1,5a]-s-triazine-4-one (J. Heterocycl. Chem. 1985, 22, 601-634) and further oxidized to the corresponding acid (e.g. by SeO2 and then oxone).

In case where Q2 of Formula (I) is an optionally substituted 1,3-pyrimidin-4-one, the building block can be synthesized according Chem. Pharm. Bull. 1982, 30, 4314-4324 (Scheme 4)

In the cyclic moiety of the product in Scheme 1 to Scheme 4, further functional group manipulation can be applied (e.g. J. March, Advanced Organic Chemistry, Wiley&Sons), e.g. palladium catalyzed halogen-cyanide exchange or nucleophilic substitution.

EXAMPLES AND METHODS

All reagents and solvents were obtained from commercial sources and used without further purification. Proton spectra (1H-NMR) were recorded on a 400 MHz or a 250 MHz NMR spectrometer in deuterated solvents. Purification by column chromatography was performed using silica gel, grade 60, 0.06-0.2 mm (chromatography) or silica gel, grade 60, 0.04-0.063 mm (flash chromatography) and suitable organic solvents as indicated in specific examples. Preparative thin layer chromatography was carried out on silica gel plates with UV detection.

Preparative Examples are directed to intermediate compounds useful in preparing the compounds of the present invention.

Preparative Example 1

Step A

If one were to treat 3-aza-spiro[5.5]undecan-9-one (Bioorg. Med. Chem. Lett. 2001, 11, 1293-1296) with a base, e.g. K2CO3 and methyliodide in dry DMF, one would obtain the title compound.

Step B

If one were to treat the title compound from Step A above with ethylcyanoacetate in acetic acid and ammonium acetate in toluene under reflux with a dean stark condenser one would obtain the title compound.

Step C

If one were to treat the title compound from Step B above with sulfur and diethylamine (0.1 eq.) in dry methanol at 50° C. overnight one would obtain the title compound.

Step D

If one were to treat the title compound from Step C similar as desribed in the Preparative Example 11, Step A one would obtain the title compound.

Step E

If one were to treat the title compound from Step D similar as desribed in the Preparative Example 2, Step C one would obtain the title compound.

Preparative Example 2

Step A

5-Amino-3-phenylisoxazole (995 mg) was suspended in dry pyridine (20 mL) and ethyloxalyl chloride (830 μL) was added under ice cooling. The ice bath was removed and the mixture was stirred for 2 d at room temperature and evaporated. The residue was diluted with ethyl acetate and subsequently washed with 10% aqueous citric acid and brine, dried and concentrated. The residue was recrystallized from ethyl acetate/cyclohexane to give the title compound (969 mg, 60%) as colourless needles. [MH]+=261.

Step B

The title compound from Step A above (548 mg) and PtO2 (115 mg) were suspended in EtOH (20 mL) and hydrogenated at atmospheric pressure for 2 h at 50° C., filtered over celite and refluxed for further 2 h, evaporated and purified by flash chromatography (cyclohexane/ethyl acetate 6:4 to 4:6) to afford the title compound as a colourless solid. [MH]+=245.

Step C

To a solution of the title compound from Step B above (105 mg) in THF (20 mL) was added 1M aqueous LiOH (1 mL). The resulting mixture was stirred at room temperature for 2 h, concentrated and neutralized with 1M aqueous HCl. The residue was filtered off and used without further purification (46 mg, 50%). [MH]+=217.

Preparative Examples 2/1 to 2/3

Following a similar procedure as described in the Preparative Example 2 except using the amine indicated in Table I.1 below, the following compounds were prepared.

TABLE I.1 Prep. Ex. # amine product yield 2/1 24%[MH]+ = 169 2/2 n.d.[MH]+ = 231 2/3 39%[MH]+ = 235

Preparative Examples 3/1 to 3/22

If one were to use the procedure as described in the Preparative Example 2 except using the amine indicated in Table I.2 below, the following compounds would be obtained.

TABLE I.2 Prep. Ex. # amine product 3/1  3/2  3/3  3/4  3/5  3/6  3/7  3/8  3/9  3/10 3/11 3/12 3/13 3/14 3/15 3/16 3/17 3/18 3/19 3/20 3/21 3/22

Preparative Example 3

Step A

A suspension of 6-amino-2,3-difluorophenol (1.0 g), K2CO3 (3 g), bromoacetylchloride (750 μL) and a catalytic amount of TBAI in dry acetonitrile was stirred at reflux overnight, evaporated and diluted with ethyl acetate, washed with 1N HCl, brine and a saturated solution of sodium hydrogen carbonate, dried and evaporated to give the title compound (1.1 g, 86%) as a brown solid [MH]+=186.

Step B The title compound of Step A above (1.1 g) was dissolved in acetic acid and bromine (1 mL) was added. The solution was stirred at room temperature overnight, then additional bromine (1 mL) was added and the temperature was elevated to 40° C. for 3 h. The solution was evaporated and diluted with ethyl acetate, washed with a aqueous solution of sodium sulfite, brine and a saturated solution of sodium hydrogen carbonate, dried, absorbed on silica and purified by flash chromatography (cyclohexane/ethyl acetate 8:2 to 7:3) to give the 5-bromo-isomer (787 mg, 50%) and 6-bromo-isomer (567 mg, 36%) as off-white solids. [MH]+=264/66.

Preparative Examples 3a to 3b

Following a similar procedure as described in the Preparative Example 3, except using the aminoalcohols indicated in Table I.3 below, the following compounds were prepared.

TABLE I.3 Prep. Ex. # aminealcohol product yield 3a n.d.[MH]+ = 246/48 3b 19%[MH]+ = 246/48

Preparative Example 4

Step A

A suspension of the title compound from the Preparative Example 3, Step B (567 mg) and CuCN (230 mg) in dry N-methyl-pyrrolidin-2-one (15 mL) was degassed under Argon and heated under microwave irradiation to 200° C. for 2 h. The mixture was concentrated, diluted with 1N HCl (100 mL) and extracted with EtOAc (200 mL). The organic layers were washed with H2O (2×200 mL) and brine (200 mL), dried (MgSO4), filtered, absorbed on silica and purified by flash chromatography (cyclohexane/ethyl acetate 7:3 to 6:5) to give the title compound as a colourless solid. [MH]+=211.

Step B

To an ice cooled solution of the title compound from Step A above in dry MeOH (20 mL) were added di-tert-butyl dicarbonate (500 mg) and NiCl2.6H2O (20 mg), followed by the careful portionwise addition of NaBH4 (320 mg). The resulting black mixture was stirred for 20 min at 0-5° C. (ice bath), then the ice bath was removed and stirring at room temperature was continued overnight. Then diethylenetriamine was added and the mixture was concentrated to dryness. The remaining residue was suspended in EtOAc, washed subsequently with 10% aqueous citric acid, saturated aqueous NaHCO3 and brine, dried (MgSO4), filtered, concentrated and purified by chromatography (silica, cyclohexane/EtOAc 7:3 to 1:1) to afford the title compound as a colourless solid (317 mg, 47% over two steps). [MNa]+=337.

Step C

The title compound from the Step B above (317 mg) was stirred in a 4M solution of HCl in 1,4-dioxane (10 mL) at room temperature overnight and then concentrated to afford the title compound (256 mg, quant.) as a colourless solid. [M-NH2Cl]+=198, [M-Cl]+=215.

Preparative Examples 4a to 4d

Following a similar procedure as described in the Preparative Example 4, Step A except using the educt indicated in Table I.4 below, the following compounds were prepared.

TABLE I.4 Prep. Ex. # educt product yield 4a n.d.[MH]+= 193 4b 93%[MH]+ = 193 4c n.d.[MH]+ = 193 4d 55%[MH]+ = 211

Preparative Examples 5a to 5f

Following a similar procedure as described in the Preparative Example 4, Step B except using the educt indicated in Table I.5 below, the following compounds were prepared.

TABLE I.5 Prep. Ex. # educt product yield 5a 32% (3 steps)[MNa]+ = 319 5b 56%[MNa]+ = 319 5c 28% (2 steps)[MNa]+ = 319 5d n.d.[MNa]+ = 325 5e 58%[MNa]+ = 301 5f 64%[MNa]+ = 337

Preparative Examples 6a to 6h

Following a similar procedure as described in the Preparative Example 4, Step C except using the educt indicated in Table I.6 below, the following compounds were prepared.

TABLE I.6 Prep. Ex. # educt product yield 6a quant.[M − Cl]+ = 197 6b quant.[M − Cl]+ = 195 6c quant.[M − Cl]+ = 197 6d quant.[M − Cl]+ = 197 6e 66% (2 steps)[M− Cl]+ = 203 6f n.d.[M− Cl]+ = 179 6g quant.[M− Cl]+ = 220 6h quant.[M− Cl]+ = 215

Preparative Example 7

Step A

To a solution of the starting material (380 mg) in dry THF was added Lawesson's reagent (660 mg) and the mixture was stirred for 4 h and then concentrated. The remaining residue was dissolved in EtOAc, washed subsequently with 10% aqueous citric acid, saturated aqueous NaHCO3 and brine, dried (MgSO4), filtered, concentrated and purified by chromatography (silica, cyclohexane/EtOAc 85:15 to 8:2) to afford the title compound as a colourless solid (312 mg, 78%). [MNa]+=317.

Preparative Example 7a

Following a similar procedure as described in the Preparative Example 7, except using the educt indicated in Table I.7 below, the following compound was prepared.

TABLE I.7 Prep. Ex. # Educt product yield 7a 87%[MH]+ = 244/46

Preparative Example 8

Step A

To a solution of 4-bromo-2-fluoro-6-nitrophenol (6.91 g) in dry DMF was added methylbromoacetate (3.3 mL), K2CO3 (7.4 g) and catalytic amounts of TBAI at 0° C. and the mixture was stirred for 2 h, allowing to reach room temperature. The mixture was concentrated, dissolved in EtOAc, washed subsequently with 1N HCl, saturated aqueous NaHCO3 and brine, dried (MgSO4), filtered, absorbed on silica and purified by chromatography (silica, cyclohexane/EtOAc 9:1 to 8:2) to afford the title compound as a colourless solid (8.2 g, 91%). [MH]+=308/10.

Step B

The title compound from Step A above (1.35 g) and tin (1.3 g) in conc. HCl (10 mL) and MeOH (2 mL) were heated to reflux for 2 h. The mixture was cooled, poured on water and the solid was filtered to give the title compound as a colourless solid (985 mg, 91%). [MH]+=246/48.

Preparative Example 8a

Step A

A solution of title compound the from Preparative Example 7a above (164 mg) and formylhydrazine (50 mg) in butanol was heated under microwave irradiation to 160° C. for 3 h, absorbed on silica and purified by flash chromatography (silica, CH2Cl2/methanol 98:2 to 95:5) to afford the title compound as a colourless solid (129 mg, 76%). [MH]+=252/54.

Preparative Example 9

Step A

A mixture of the title compound from the Preparative Example 8a (125 mg), Zn(CN)2 (44 mg) and Pd(PPh3)4 (40 mg) in dry DMF (10 mL) was degassed and heated at 85° C. under an argon atmosphere overnight. The mixture was concentrated, diluted with 1N HCl, sonificated, filtered and washed with water, few methanol and then pentane to afford the title compound (100 mg, quant.) as a colourless solid. [MH]+=199.

Preparative Example 9a

Following a similar procedure as described in the Preparative Example 9, except using the educt indicated in Table I.8 below, the following compound was prepared.

TABLE I.8 Prep. Ex. # educt product yield 9a n.d.[MH]+ = 175

Preparative Example 10

Step A

The title compound from the Preparative Example 7 (123 mg) was treated as described in Monatsh. Chem. 1989, 120, 81-84 to afford the title compound as a colourless solid (120 mg, 89%). [MNa]+=342.

Preparative Example 11

Step A

A mixture of benzyl-4-formyltetrahydro-1(2H)-pyridocarboxylate (750 mg, 3 mmol), methylvinylketone (270 μL, 3.3 mmol) and sulphuric acid (50 μL) in 5 mL benzene were heated at reflux in presence of a Dean-Stark. After 4 h, 270 μL of methylvinylketone were added and the heating was pursued for 18 h. The reaction mixture was dissolved in dichloromethane and washed with a saturated solution of NaHCO3. The organic layer was separated, dried over MgSO4, filtered and the volatile components removed under reduced pressure. A purification by flash chromatography (cyclohexane/ethyl acetate 7/3) afforded the title product (0.114 g, 12%) as a yellow oil. [MH]+=300.

Step B

The title compound from Step A above (0.29 g) was placed in EtOH in presence of Pd/C (10%). The reaction mixture was stirred at room temperature overnight under an hydrogen atmosphere. The mixture was filtrated over celite and ethanol was removed under reduced pressure to afford the hydrogenated double bound product (0.192 g, 66%). [MH]+=302.

Step C

The title compound from Step B above (190 mg), ethyl cyanoacetate (90 μL), acetic acid (25 μL) and ammonium acetate (10 mg) in toluene (5 mL) were heated to reflux in presence of a Dean-Stark overnight. After concentration of the mixture, a purification by chromatography on silica (cyclohexane/EtOAc 8/2) afforded a yellow oil (142 mg, 57%). [MH]+=397.

Step D

A mixture of the title compound from Step C above (140 mg) and sulfur (17 mg) in EtOH (3 mL) were heated at 50° C. Diethylamine (25 μL) was added slowly and the mixture was stirred at 50° C. for 3 h. After concentration of the mixture, a purification by chromatography (cyclohexane/EtOAc 8/2) afforded a yellow oil (128 mg, 63%). [MH]+=429.

Step E

The title compound from Step D above (125 mg) was dissolved in a 4M solution of HCl in 1,4-dioxane (5 mL) and nitriloacetic acid ethyl ester (0.045 mL) was added. The mixture was stirred at 50° C. for 3 hours, concentrated and purified by extraction with ethyl acetate from an aqueous solution. The redidue was purified by flash chromatography (cyclohexane/EtOAc 5/5) to afford the desired product (101 mg, 72%). [MH]+=482.

Step F

To a solution of the title compound from Step E above (100 mg) in THF (1 mL) was added 1M aqueous LiOH (0,65 mL). The resulting mixture was stirred at room temperature 1,5 h, concentrated and neutralized with 1M aqueous HCl. The residue was filtered off and used without further purification (89 mg, 95%). [MH]+=454.

Preparative Example 12

Step A

The starting anhydride (341 mg, 1.92 mmol) was suspended in MeOH (2 mL) and heated in a microwave at 60° C. for 30 min with stirring. The reaction mixture was concentrated to produce 381 mg of desired ester acid as a white solid. [M-H]−=209.

Step B

To a mixture of the acid from step A above (203 mg, 0.966 mmol) and triethyl amine (0.27 mL, 0.20 g, 1.94 mmol) in t-BuOH (2 mL) was added DPPA (0.42 mL, 0.53 g, 1.94 mmol) and the reaction was heated in microwave at 100° C. for 10 min with stirring. Typical aqueous workup and chromatography produced 216 mg of the amino ester. [MH]+=282.

Step C

A mixture of the product from Step B above (55 mg, 0.195 mmol) and ethyl cyanoformate (58 μL, 58 mg, 0.59 mmol) in 4M HCl in dioxane (1 mL) and dioxane (2 mL) was heated in microwave at 120° C. for 4 h with stirring. The reaction mixture was concentrated, and the CH2Cl2 washes were combined and concentrated to give 66 mg of desired ester as a yellow solid. [MH]+=249.

Preparative Examples 12a to 12c

If one were to follow a similar procedure as in Preparative Example 12, but starting with the amino esters listed in the Table I.9 below, one would obtain the products listed.

TABLE I.9 Prep. Ex. # amino ester product 12a 12b 12c

Preparative Example 13

Step A

The commercially available compound above (500 mg) was dissolved in a 4M solution of HCl in 1,4-dioxane (5 mL) and nitriloacetic acid ethyl ester (0.26 mL) was added. The mixture was stirred at 50° C. for 3 hours, concentrated and purified by extraction with ethyl acetate from an aqueous solution to afford the desired product (590 mg, >99%). [MH]+=337.

Preparative Example 14

Step A

To a solution of the Preparative Example 13 above (50 mg) in THF (0,5 mL) was added 1M aqueous LiOH (0,45 mL). The resulting mixture was stirred at room temperature 2 h, concentrated and neutralized with 1M aqueous HCl. The residue was filtered off and used without further purification (19 mg, 42%). [MH]+=309.

Example 1

Step A

To a solution of the title compound from Preparative Example 11, Step F above (85 mg), EDCI (72 mg) and HOAt (25 mg) in DMF (3 mL) were added N-methylmorpholine (100 μL) and hydrochloride salt of the 6-Aminomethyl-4H-benzo[1,4]oxazin-3-one (48 mg). The mixture was stirred overnight and then concentrated. The remaining residue was suspended in 10% aqueous citric acid and the residue was filtered to afford the title compound as an off white solid (115 mg, >99%). [MH]+=614.

Examples 1/1 to 1/33

Following a similar procedure as described in the Example 1 except using the amines and acids indicated in Table II.1 below, the following compounds were prepared. If marked with *, the workup was accomplished via purification by RP-18-chromatography. If not otherwise stated, the acid and amine building block is commercially available or the synthesis is described in the Preparative Examples.

TABLE II.1 Ex. # amine, acid product yield 1/1    68%[MH]+ = 377 1/2    14%[MH]+ = 329 1/3    n.d.[MH]+ = 391 1/4    77%[MH]+ = 351 1/5    57%[MH]+ = 365 1/6    80%[MH]+ = 365 1/7    76%[MH]+ = 351 1/8    74%[MH]+ = 365 1/9    98%[MH]+ = 371 1/10   53%[MH]+ = 369 1/11   38%[MH]+ = 387 1/12   74%[MH]+ = 403 1/13   78%[MH]+ = 352 1/14   86%[MH]+ = 370 1/15   81%[MH]+ = 386 1/16   62%[MH]+ = 367 1/17   63%[MH]+ = 401 1/18   74%[MH]+ = 375 1/19   70%[MH]+ = 409 1/20   89%[MH]+ = 387 1/21   74%[MH]+ = 421 1/22   79%[MH]+ = 369 1/23   90%[MH]+ = 387 1/24   89%[MH]+ = 403 1/25   73%[MH]+ = 369 1/26   88%[MH]+ = 403 1/27   72%[MH]+ = 387 1/28   95%[MH]+ = 421 1/29   n.d.[MH]+ = 393 1/30   92%[MH]+ = 392 1/31   92%[MH]+ = 428 1/32   n.d.[MH]+ = 449 1/33   54%[MH]+ = 395 1/34   n.d.[MH]+ = 365

Examples 2/2 to 2/46

If one were to treat the acid as described in the Example 1 except using the amines and acids indicated in Table II.2 below, the following compounds would be obtained.

TABLE II.2 Ex. # amine, acid product 2/1    2/2    2/3    2/4    2/5    2/6    2/7    2/8    2/9    2/10   2/11   2/12   2/13   2/14   2/15   2/16   2/17   2/18   2/19   2/20   2/21   2/22   2/23   2/24   2/25   2/26   2/27   2/28   2/29   2/30   2/31   2/32   2/33   2/34   2/35   2/36   2/37   2/38   2/39   2/40   2/41   2/42   2/43   2/44   2/45   2/46  

Example 3

Step A

A mixture of ester from the Preparative Example 13, Step C (66 mg, 0.27 mmol), 6-(aminomethyl)-2H-benzo[b][1,4]oxazin-3(4H)-one HCl salt (58 mg, 0.27 mmol) and triethyl amine (74 μL, 54 mg, 0.53 mmol) in EtOH (1.5 mL) was heated in microwave at 160° C. for 1 h with stirring. The reaction mixture was concentrated and purified by reverse phase HPLC to produce the desired product. [MH]+=381

Example 4

Step A

A mixture of (4-oxo-3,4-dihydro-quinazolin-2-yl)-acetic acid methyl ester (28.8 mg, 0.132 mmol), 6-(aminomethyl)-2H-benzo[b][1,4]oxazin-3(4H)-one HCl salt (34.9 mg, 0.163 mmol), and triethylamine (55 μL, 40 mg, 0.40 mmol) in DMF (1 ml) was heated in a microwave at 160° C. for 5 min, then 180° C. for 1 h. Typical aqueous workup and purification provided 7.1 mg of the desired product as a pale yellow solid. [MH]+=365.

Example 4/1 to 4/3

If one were to follow a similar procedure as that described in Examples 3 except using the esters and amines indicated in Table II.3 below, the following compounds would be obtained.

TABLE II.3 Ex. # amine, ester product 4/1   4/2   4/3  

Example 5

Step A

A mixture of (3-oxo-3,4-dihydro-2H-benzo[1,4]oxazine-6-carbaldehyde (37 mg) and 2-hydrazino-3H-quinazolin-4-one (36.8 mg) in DMF (1 mL) was stirred at room temperature overnight, diluted with water and filtered. The remaining solid was slurried in methanol, filtered, washed with few ethyl acetate and dried to afford the title compound (44 mg, 63%) as a yellow solid. [MH]+=336.

Example 6

Step A

A mixture of (3-oxo-3,4-dihydro-2H-benzo[1,4]oxazine-6-sulfonyl chloride (36 mg) and 2-hydrazino-3H-quinazolin-4-one (29 mg) in pyridine (1 mL) was stirred at room temperature overnight, evaporated, diluted with 1N HCl and filtered. The remaining solid washed with water and dried to afford the title compound (38 mg, 67%) as a brown solid. [MH]+=388.

Example 1700 Assay for Determining MMP-13 Inhibition

The typical assay for MMP-13 activity is carried out in assay buffer comprised of 50 mM Tris, pH 7.5, 150 mM NaCl, 5 mM CaCl2 and 0.05% Brij-35. Different concentrations of tested compounds are prepared in assay buffer in 50 μL aliquots. 10 μL of a 50 nM stock solution of catalytic domain of MMP-13 enzyme (produced by Alantos or commercially available from Invitek (Berlin), Cat.#30100812) is added to the compound solution. The mixture of enzyme and compound in assay buffer is thoroughly mixed and incubated for 10 min at room temperature. Upon the completion of incubation, the assay is started by addition of 40 μL of a 12.5 μM stock solution of MMP-13 fluorescent substrate (Calbiochem, Cat. No. 444235). The time-dependent increase in fluorescence is measured at the 320 nm excitation and 390 nm emission by automatic plate multireader. The IC50 values are calculated from the initial reaction rates.

Example 1701 Assay for Determining MMP-3 Inhibition

The typical assay for MMP-3 activity is carried out in assay buffer comprised of 50 mM MES, pH 6.0, 10 mM CaCl2 and 0.05% Brij-35. Different concentrations of tested compounds are prepared in assay buffer in 50 μL aliquots. 10 μL of a 100 nM stock solution of the catalytic domain of MMP-3 enzyme (Biomol, Cat. No. SE-1 09) is added to the compound solution. The mixture of enzyme and compound in assay buffer is thoroughly mixed and incubated for 10 min at room temperature. Upon the completion of incubation, the assay is started by addition of 40 μL of a 12.5 μM stock solution of NFF-3 fluorescent substrate (Calbiochem, Cat. No. 480455). The time-dependent increase in fluorescence is measured at the 330 nm excitation and 390 nm emission by an automatic plate multireader. The IC50 values are calculated from the initial reaction rates.

Example 1702 Assay for Determining MMP-8 Inhibition

The typical assay for MMP-8 activity is carried out in assay buffer comprised of 50 mM Tris, pH 7.5, 150 mM NaCl, 5 mM CaCl2 and 0.05% Brij-35. Different concentrations of tested compounds are prepared in assay buffer in 50 μL aliquots. 10 μL of a 50 nM stock solution of activated MMP-8 enzyme (Calbiochem, Cat. No. 444229) is added to the compound solution. The mixture of enzyme and compound in assay buffer is thoroughly mixed and incubated for 10 min at 37° C. Upon the completion of incubation, the assay is started by addition of 40 μL of a 10 μM stock solution of OmniMMP fluorescent substrate (Biomol, Cat. No. P-126). The time-dependent increase in fluorescence is measured at the 320 nm excitation and 390 nm emission by an automatic plate multireader at 37° C. The IC50 values are calculated from the initial reaction rates.

Example 1703 Assay for Determining MMP-12 Inhibition

The typical assay for MMP-12 activity is carried out in assay buffer comprised of 50 mM Tris, pH 7.5, 150 mM NaCl, 5 mM CaCl2 and 0.05% Brij-35. Different concentrations of tested compounds are prepared in assay buffer in 50 μL aliquots. 10 μL of a 50 nM stock solution of the catalytic domain of MMP-12 enzyme (Biomol, Cat. No. SE-138) is added to the compound solution. The mixture of enzyme and compound in assay buffer is thoroughly mixed and incubated for 10 min at room temperature. Upon the completion of incubation, the assay is started by addition of 40 μL of a 12.5 μM stock solution of OmniMMP fluorescent substrate (Biomol, Cat. No. P-126). The time-dependent increase in fluorescence is measured at the 320 nm excitation and 390 nm emission by automatic plate multireader at 37° C. The IC50 values are calculated from the initial reaction rates.

Example 1704 Assay for Determining Aggrecanase-1 Inhibition

The typical assay for aggrecanase-1 activity is carried out in assay buffer comprised of 50 mM Tris, pH 7.5, 150 mM NaCl, 5 mM CaCl2 and 0.05% Brij-35. Different concentrations of tested compounds are prepared in assay buffer in 50 μL aliquots. 10 μL of a 75 nM stock solution of aggrecanase-1 (Invitek) is added to the compound solution. The mixture of enzyme and compound in assay buffer is thoroughly mixed. The reaction is started by addition of 40 μL of a 250 nM stock solution of aggrecan-IGD substrate (Invitek) and incubation at 37° C . for exact 15 min. The reaction is stopped by addition of EDTA and the samples are analysed by using aggrecanase ELISA (Invitek, InviLISA, Cat. No. 30510111) according to the protocol of the supplier. Shortly: 100 μL of each proteolytic reaction are incubated in a pre-coated micro plate for 90 min at room temperature. After 3 times washing, antibody-peroxidase conjugate is added for 90 min at room temperature. After 5 times washing, the plate is incubated with TMB solution for 3 min at room temperature. The peroxidase reaction is stopped with sulfurous acid and the absorbance is red at 450 nm. The IC50 values are calculated from the absorbance signal corresponding to residual aggrecanase activity.

Claims

1. A compound having Formula (I): wherein:

R4 in each occurrence is independently selected from R10, hydrogen, alkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, halo, haloalkyl, CF3, (C0-C6)-alkyl-COR10, (C0-C6)-alkyl-OR10, (C0-C6)-alkyl-NR10R11, (C0-C6)-alkyl-NO2, (C0-C6)-alkyl-CN, (C0-C6)-alkyl-S(O)yOR10, (C0-C6)-alkyl-S(O)yNR10R11, (C0-C6)-alkyl-NR10CONR11SO2R30, (C0-C6)-alkyl-S(O)xR10, (C0-C6)-alkyl-OC(O)R10, (C0-C6)-alkyl-OC(O)NR10R11, (C0-C6)-alkyl-C(═NR10)NR10R11, (C0-C6)-alkyl-NR10C(═NR11)NR10R11, (C0-C6)-alkyl-C(O)OR10, (C0-C6)-alkyl-C(O)NR10R11, (C0-C6)-alkyl-C(O)NR10SO2R11, (C0-C6)-alkyl-C(O)—NR11—CN,
O—(C0-C6)-alkyl-C(O)NR10R11, S(O)x—(C0-C6)-alkyl-C(O)OR10, S(O)x—(C0-C6)-alkyl-C(O)NR10R11, (C0-C6)-alkyl-C(O)NR10—(C0-C6)-alkyl-NR10R11, (C0-C6)-alkyl-NR10—C(O)R10, (C0-C6)-alkyl-NR10—C(O)OR10, (C0-C6)-alkyl-NR10—C(O)—NR10R11, (C0-C6)-alkyl-NR10—S(O)yNR10R11, (C0-C6)-alkyl-NR10—S(O)yR10, O—(C0-C6)-alkyl-aryl and O—(C0-C6)-alkyl-heteroaryl,
wherein each R4 group is optionally substituted one or more times, or
wherein each R4 group is optionally substituted by one or more R14 groups;
R8 is selected from R10 or optionally R8 and X1 when taken together with the nitrogen and sp2-carbon atom to which they are attached complete a 5- to 8-membered unsaturated or partially unsaturated heterocycle optionally containing additional heteroatoms selected from O, S(O)x, N or NR50 and which is optionally substituted one or more times;
R9 in each occurrence is independently selected from R10, hydrogen, alkyl, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, halo, CHF2, CF3, OR10, SR10, COOR10, CH(CH3)CO2H, (C0-C6)-alkyl-COR10, (C0-C6)-alkyl-OR10, (C0-C6)-alkyl-NR10R11, (C0-C6)-alkyl-NO2, (C0-C6)-alkyl-CN, (C0-C6)-alkyl-S(O)yOR10, (C0-C6)-alkyl-P(O)2OH, (C0-C6)-alkyl-S(O)yNR10R11, (C0-C6)-alkyl-NR10CONR11SO2R30, (C0-C6)-alkyl-S(O)xR10, (C0-C6)-alkyl-OC(O)R10, (C0-C6)-alkyl-OC(O)NR10R11, (C0-C6)-alkyl-C(═NR10)NR10R11, (C0-C6)-alkyl-NR10C(═NR11)NR10R11, (C0-C6)-alkyl-NR10C(═N—CN)NR10R11, (C0-C6)-alkyl-C(═N—CN)NR10R11, (C0-C6)-alkyl-NR10C(═N—NO2)NR10R11, (C0-C6)-alkyl-C(⊚N—NO2)NR10R11, (C0-C6)-alkyl-C(O)OR10, (C0-C6)-alkyl-C(O)NR10R11, (C0-C6)-alkyl-C(O)NR10SO2R11, C(O)NR10—(C0-C6)-alkyl-heteroaryl, C(O)NR10—(C0-C6)-alkyl-aryl, S(O)2NR10—(C0-C6)-alkyl-aryl, S(O)2NR10—(C0-C6)-alkyl-heteroaryl, S(O)2NR10-alkyl, S(O)2-(C0-C6)-alkyl-aryl, S(O)2-(C0-C6)-alkyl-heteroaryl, (C0-C6)-alkyl-C(O)—NR11—CN, O—(C0-C6)-alkyl-C(O)NR10R11, S(O)x—(C0-C6)-alkyl-C(O)OR10, S(O)x—(C0-C6)-alkyl-C(O)NR10R11, (C0-C6)-alkyl-C(O)NR10—(C0-C6)-alkyl-NR10R11, (C0-C6)-alkyl-NR10—C(O)R10, (C0-C6)-alkyl-NR10—C(O)OR10, (C0-C6)-alkyl-NR10—C(O)—NR10R11, (C0-C6)-alkyl-NR10—S(O)yNR10R11, (C0-C6)-alkyl-NR10—S(O)yR11, O—(C0-C6)-alkyl-aryl and O—(C0-C6)-alkyl-heteroaryl, wherein each R9 group is optionally one or more times substituted;
R10 and R11 in each occurrence are independently selected from hydrogen, alkyl, cycloalkyl, cycloalkylalkyl, heterocycloalkyl, fluoroalkyl, heterocycloalkylalkyl, haloalkyl, alkenyl, alkynyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl and aminoalkyl, wherein alkyl, cycloalkyl, cycloalkylalkyl, heterocycloalkyl, fluoroalkyl, heterocycloalkylalkyl, alkenyl, alkynyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl and aminoalkyl are optionally substituted one or more times, or R10 and R11 when taken together with the nitrogen to which they are attached complete a 3- to 8-membered ring containing carbon atoms and optionally containing a heteroatom selected from O, S(O)x, or NR50 and which is optionally substituted one or more times;
R14 is independently selected from hydrogen, alkyl, arylalkyl, cycloalkylalkyl, heteroarylalkyl, heterocyclylalkyl and halo, wherein alkyl, arylalkyl, cycloalkylalkyl, heteroarylalkyl and heterocyclylalkyl are optionally substituted one or more times;
R17 is selected from R9, alkenyl, alkynyl, bicycloalkyl, heterobicycloalkyl, spiroalkyl, spiroheteroalkyl, cycloalkyl fused aryl, heterocycloalkyl fused aryl, cycloalkyl fused heteroaryl, heterocycloalkyl fused heteroaryl or a bicyclic or tricyclic fused ring system, wherein at least one ring is partially saturated, and
wherein each R17 group is optionally substituted one or more times, or
wherein each R17 group is optionally substituted one or more R9 groups;
R30 is selected from alkyl and (C0-C6)-alkyl-aryl, wherein alkyl and aryl are optionally substituted;
R50 in each occurrence is independently selected from hydrogen, alkyl, aryl, heteroaryl, C(O)R80, C(O)NR80R81, SO2R80 and SO2NR80R81, wherein alkyl, aryl, and heteroaryl are optionally substituted one or more times;
R80 and R81 in each occurrence are independently selected from hydrogen, alkyl, cycloalkyl, cycloalkylalkyl, heterocycloalkyl, fluoroalkyl, heterocycloalkylalkyl, haloalkyl, alkenyl, alkynyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl and aminoalkyl, wherein alkyl, cycloalkyl, cycloalkylalkyl, heterocycloalkyl, fluoroalkyl, heterocycloalkylalkyl, alkenyl, alkynyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl and aminoalkyl are optionally substituted, or R80 and R81 when taken together with the nitrogen to which they are attached complete a 3- to 8-membered ring containing carbon atoms and optionally a heteroatom selected from O, S(O)x, NH, and N(alkyl) and which is optionally substituted one or more times;
Lc is selected from a single bond or an acyclic, straight or branched, saturated or unsaturated hydrocarbon chain having 1 to 10 carbon atoms, optionally containing 1 to 3 groups independently selected from —S—, —O—, NR10—, —NR10CO—, —CONR10—, —S(O)x—, —SO2NR10—, —NR10SO2—, NR10SO2NR10, —NR10CONR10—, —OC(O)NR10—, —NR10C(O)O—, which replace single carbon atoms, which in case that more than two carbon atoms are replaced are not adjacent, and wherein the hydrocarbon chain is optionally substituted one or more times;
Ld is selected from a single bond or a straight or branched, saturated or unsaturated hydrocarbon chain having 1 to 10 carbon atoms, optionally containing 1 to 3 groups independently selected from —O—, —NR10—, —S(O)x—, —NR10C(X1)—, —C(X1)NR10—, —SO2NR10—, —NR10SO2—, —O—SO2—, —SO2—O—, —NR10SO2NR10—, —NR10C(X1)NR10—, —OC(X1)NR10—, —NR10C(X1)O—, —OC(X1)—, —C(X1)O—, -Q2, —NR10-Q2, -Q2-NR10—, —C(X1)-Q2, -Q2-C(X1)—, —O-Q2-, —S(O)x-Q2, and -Q2-S(O)x— which replace single carbon atoms, which in case that more than two carbon atoms are replaced are not adjacent, and wherein the hydrocarbon chain is optionally substituted one or more times;
Q1 is a 4- to 8-membered ring selected from cycloalkyl, heterocycloalkyl, bicycloalkyl, heterobicycloalkyl or a 5- or 6-membered ring selected from aryl and heteroaryl, wherein cycloalkyl, heterocycloalkyl, bicycloalkyl, heterobicycloalkyl, aryl and heteroaryl are optionally substituted one or more times by R4 and optionally a substituent of Q1 is linked with Ld to complete a 3- to 8-membered ring containing carbon atoms and optionally heteroatoms selected from O, S(O)x, —NH, and —N(alkyl) wherein this new ring is optionally substituted one or more times;
Q2 is independently selected from an aromatic, partially aromatic or non-aromatic cyclic, bicyclic or multicyclic system containing 0 to 8 heteroatoms selected from N, O and S(O)x, which is optionally substituted one or more times with R4 and wherein the cycles are optionally spiro fused and optionally a substituent of Q2 is linked with Ld to complete a 3- to 8-membered ring containing carbon atoms and optionally heteroatoms selected from O, S(O)x, —NH, and —N(alkyl) wherein this new ring is optionally substituted one or more times;
X1 is independently selected from O, S, NR10, NOR10, N—CN, NCOR10, N—NO2, and N—SO2R10;
Y is selected from O, S(O)x, CR10OR11, and NR10;
Z1 is independently selected from C, S, S═O, PR10 and P—OR10;
w is independently selected from 0 to 3;
x is independently selected from 0 to 2;
y is selected from 1 and 2; and
N-oxides, pharmaceutically acceptable salts, prodrugs, formulations, polymorphs, racemic mixtures and stereoisomers thereof.

2. The compound of claim 1, wherein the group Q2-Lc-R17 is selected from: wherein:

R5 in each occurrence is independently selected from hydrogen, alkyl, C(O)NR10R11, aryl, arylalkyl, SO2NR10R11 and C(O)OR10, wherein alky, aryl and arylalkyl are optionally substituted one or more times;
E is independently selected from a bond, CR10R11, O, NR5, S, S═O, S(═O)2, C(═O), N(R10)(C═O), (C═O)N(R10), N(R10)S(═O)2, S(═O)2N(R10), C═N—OR11, —C(R10R11)C(R10R11)—, —CH2—W1— and
Q3 is selected from an aromatic, partially aromatic or non-aromatic cyclic, bicyclic or multicyclic system containing 0 to 8 heteroatoms selected from N, O and S(O)x, which is optionally substituted one or more times with R4;
Q4 is selected from an aromatic, partially aromatic or non-aromatic cyclic, bicyclic or multicyclic system containing 0 to 8 heteroatoms selected from N, O and S(O)x, which is optionally substituted one or more times with R4;
U is independently selected from C(R5R10), NR5, O, S, S═O and S(═O)2;
W1 is independently selected from O, NR5, S, S═O, S(═O)2, N(R11)(C═O), N(R10)S(═O)2 and S(═O)2N(R10); and
g and h are independently selected from 0-2.

3. The compound of claim 2, wherein

Q4 is selected from an aromatic, partially aromatic or non-aromatic bicyclic or multicyclic system containing 0 to 8 heteroatoms selected from N, O and S(O)x, which is optionally substituted one or more times with R4.

4. The compound of claim 3 selected from: wherein:

R8 is selected from R10 or optionally R8and X1 when taken together with the nitrogen and sp2-carbon atom to which they are attached complete a 5- to 8-membered unsaturated or partially unsaturated heterocycle optionally containing additional heteroatoms selected from O, S(O)x, N or NR50 and which is optionally substituted one or more times;
L, M and T are independently selected from CR9 and N;
X1 is independently selected from O, S, NR10, NOR10, N—CN, NCOR10, N—NO2, and N—SO2R10.

5. A pharmaceutical composition comprising

an effective amount of a compound according to claim 1; and
a pharmaceutically-acceptable carrier.

6. A method of treating rheumatoid arthritis, osteoarthritis or inflammation comprising administering a therapeutically-effective amount of a compound according to claim 1.

Patent History
Publication number: 20080221095
Type: Application
Filed: Mar 7, 2008
Publication Date: Sep 11, 2008
Inventors: Christian Gege (Mauer), Carine Chevrier (Munchen), Brian M. Gallagher (Merrimac, MA), Arthur Taveras (Southborough, MA)
Application Number: 12/075,070