PLANTS WITH IMPROVED PHOTOSYNTHETIC CARBON FIXATION CAPACITY

Abstract

Plants are provided with increased ribulose-1,5-bisphosphate (RuBP) regeneration capacity during the Calvin cycle through increased expression of sedoheptulose 1,7 bisphosphatase, in combination with reduced photo-respiratory losses through expression of glycolate catabolizing enzymes. Such plants have a greater growth rate and/or improved biomass and/or increased carbon fixation compared to untreated plants, or plants comprising only one of the features above.

Description

FIELD OF THE INVENTION

The inventions herein described relate to the agricultural field, more specifically to methods of improving photosynthesis in plants, including crop plants as well as plants obtained by such methods. Specifically, the invention relates to plants with increased ribulose-1,5-bisphosphate (RuBP) regeneration capacity during the Calvin cycle through increased expression of seduheptulose 1,7 biosphosphatase, in combination with reduced photo-respiratory losses through expression of glycolate catabolizing enzymes. Such plants have a greater growth rate and/or improved biomass and/or increased carbon fixation compared to untreated plants, or plants comprising only one of the characteristics above described.

BACKGROUND ART

Crop productivity is influenced by many factors, among which are, on the one hand factors influencing the capacity of the plant to produce biomass (photosynthesis, nutrient and water uptake), and on the other hand factors influencing the capacity of the plant to resist ccrtain stresses, like biotic stresses (insects, fungi, viruses . . . ) or abiotic stresses (drought, salinity . . . ).

One important factor influencing the production of biomass is photosynthesis. Photosynthesis is the mechanism through which plants capture atmospheric carbon dioxide and fix it into carbon skeletons that are used for biosynthesis. The vast majority of plant species fix atmospheric CO₂ using the enzyme ribulose bisphosphate carboxylase/oxygenase (Rubisco) in the Calvin-Benson cycle. The first stable product of this cycle is a three-carbon compound, phosphoglycerate (3-PGA), and thus, this process is referred to as the C3-cycle. Regeneration of the RuBP involves eight enzymes including sedoheptulose 1,7-bisphosphatase (SBPase). Small reductions in the SBPase resulted in a decrease in CO₂ fixation and growth, identifying this enzyme as a control point in the C3 cycle. A major problem with the C3 cycle is the enzyme Rubisco. Rubisco is not only an inefficient enzyme with a low turnover number, but it also catalyses two competing reactions: carboxylation and oxygenation of RuBP. The oxygenation reaction directs the flow of carbon through the photorespiratory pathway and and can result in losses of between 25-30% of the carbon fixed. Environmental variables, such as high temperature and drought, can result in an increase in the oxygenase reaction. (for a review see Raines 2011 Plant Physiology vol 155, pp 36-42).

To improve crop photosynthesis, one strategy is to overcome the oxygenase reaction of Rubisco by creating a photorespiratory bypass in the chloroplast through expression of glycolate catabolizing enzymes.

WO2003/100066 relates to a method for the production of plants with suppressed photorespiration and improved CO₂ fixation. In particular, the invention relates to a re-use of phosphoglycolate produced in photorespiration. The reaction product will be converted to a component that may be reintegrated into the plant assimilatory metabolism inside the chloroplast. This is accomplished by the transfer of genes derived from glycolate-utilizing pathways from bacteria, algae, plants and/or animals including humans into the plant nuclear and/or plastidial genome. The method leads to a reduction of photorespiration in C3 plants and by this will be of great benefit for food production especially but not exclusively under non-favourable growth conditions.

WO2010/012796 relates to a method for stimulating the growth of the plants and/or improving the biomass production and/or increasing the carbon fixation by the plant comprising introducing into a rice plant cell, rice plant tissue or rice plant one or more nucleic acids, wherein the introduction of the nucleic acid(s) results inside the chloroplast of a de novo expression of one or more polypeptides having the enzymatic activity of a glycolate dehydrogenase.

WO2011/095528 relates to a method for stimulating the growth of the plants and/or improving the biomass production and/or increasing the carbon fixation by the plant comprising introducing into a plant cell, plant tissue or plant one or more nucleic acids, wherein the introduction of the nucleic acid(s) results inside the chloroplast of a de novo expression of one or more polypeptides having the enzymatic activity of a glycolate dehydrogenase made up from translationally fused subunits of bacterial multi-subunit glycolatc dehydrogenase enzymes.

Kebeish et al. (2007 Nature Biotechnology Vol 25, pp 593-599) reported that the photorespiratory losses in Arabidopsis thaliana can be alleviated by introducing into chloroplasts a bacterial pathway for the catabolism of the photorespiratory substrate, glycolate. The authors first targeted the three subunits of Escherichia coli glycolate dehydrogenase to Arabidopsis thaliana chloroplasts and then introduced the Escherichia coli glyoxylatc carboligase and Escherichia coli tartronic semialdehyde reductase to complete the pathway that converts glycolatc to glycerate in parallel with the endogenous photorespiratory pathway. This step-wise nuclear transformation with the five Escherichia coli genes leads to Arabidopsis plants in which chloroplastic glycolate is converted directly to glycerate. These transgenic plants grew faster, produced more shoot and root biomass, and contained more soluble sugars. An effect was also visible but to a lesser extent in Arabidopsis plants that overexpressed only the three subunits of the glycolate dehydrogenase.

Nölke et al. (2014 Plant biotechnology Journal, Vol 12, pp 734-742) described the expression of a recombinant glycolate dehydrogenase polyprotein in potato (Solanum tuberosum) plastids which strongly enhances photosynthesis and tuber yield.

Another strategy to try to improve crop photosynthesis could be the overexpression of sedoheptulose 1,7-bisphosphatase to increase the RuBP regenerative capacity of the Calvin cycle.

WO00/70062 describes expression of sedoheptulose 1,7-bisphosphatase in transgenic plants. Sedoheptulose 1,7-bisphosphatase (SBPase) is an enzyme catalyzing the reaction converting sedoheptulose 1,7-bisphosphate into sedoheptulose 7-phosphate. This enzyme is located in the chloroplast in leaves and stems. Overexpression of SBPasc in transgcnic plants is provided to improve plant yield by increasing leaf starch biosynthetic ability and sucrose production. Deregulated variants of the enzymes are also provided.

Miyagawa et al. (2001 Nature Biotechnology Vol 19 pp 965-969 reported that overexpression of cyanobacterial fructose 1,6-/sedoheptulose 1,7-bisphosphatase in tobacco enhances photosynthesis and growth.

Lefebvre et al. (2005 Plant Physiology Vol. 138 pp 451-460) reported that increased sedoheptulose 1,7-bisphosphatase activity in transgenic tobacco plants stimulates photosynthesis and growth at an early stage in development.

Lawson et al. (2006 Plant, Cell and Environment Vol 29, pp 48-58) described that decreasing SBPase alters growth and development in transgenic tobacco plants.

Tamoi et al (2006 Plant Cell Physiol, 47: 380-390) described the contribution of Fructose-1,6-bisphosphatase and S edoheptulose-1,7-bisphosphatase to the photosynthetic rate and carbon flow in the calvin cycle in transgenic plants.

Feng et al. (2007 Plant Cell Reporter Vol 26, pp 1635-1646) reported overexpression of SBPase enhancing photosynthesis under high temperature stress in transgenic rice plants. In contrast to the results with tobacco, increasing SBPase activity in rice did not lead to increasing photosynthesis or growth. However, when the plants were subjected to heat or salt stress, higher photosynthesis rates were found in the transgenic plants, compared to wild-type controls under similar conditions.

There is therefore still a need for an efficient method for increasing the carbon fixation in plants, particularly crop plants, which increases the photosynthetic carbon assimilation and stimulates the growth of the plant and/or improves biomass and/or seed production.

SUMMARY OF THE INVENTION

In one embodiment of the invention, a method is provided for improving the assimilation of carbon in a plant comprising the steps of providing cells of the plant with increased RuBP regeneration capacity; and providing cells of the plant with glycolate catabolizing enzymes. Conveniently this may be achieved by inserting into the genome of a plant a nucleic acid encoding an enzyme comprising sedoheptulose 1,7-bisphosphatase activity, from a source heterologous with respect to the plant into which the nucleic acid is inserted, such as a a plant species heterologous with respect to the plant into which the structural nucleic acid is inserted, and inserting into the genome of a plant one or more nucleic acids encoding one or more polypeptides having the enzymatic activity of glycolate dehydrogenase, wherein the one or more polypeptides are located in chloroplasts of the plants.

The nucleic acid encoding an enzyme comprising sedoheptulose 1,7-bisphosphatase activity may be operably linked to a plant-expressible promoter, such as the promoter region of the ribulosc-bisphosphatc carboxylasc small subunit gene of Oryza sativa, and optionally to a 3′ end region involved in transcription termination and/or polyadenylation. The enzyme comprising sedoheptulose 1,7-bisphosphatase activity may further comprise an amino acid sequence targeting the enzyme comprising sedoheptulose 1,7-bisphosphatase activity to the chloroplast or the chloroplast membrane or the nucleic acid encoding an enzyme comprising sedoheptulose 1,7-bisphosphatase activity may be integrated in the chloroplast genome of cells of the plant.

The one or more nucleic acids encoding one or more polypeptides having the enzymatic activity of glycolate dehydrogenase may be derived from the E. coli glc operon, such as polypeptides comprising an amino acid sequence having at least 60% sequence identity to the amino acid sequence of SEQ ID No 3, SEQ ID No 4 and SEQ ID No 5, optionally forming a multi-subunit fusion protein. The polypeptide may also be a polypeptide having the enzymatic activity of a glycolate dehydrogenase derived from Chlamydomonas or Synechocystis, including a polypeptide having at least 60% sequence identity to the amino acid sequence of SEQ ID NO 6, SEQ ID No 7 or SEQ ID No 8. In another embodiment, the polypeptide may be an Arabidopsis glycolate dehydrogenase, including a polypeptide comprising an amino acid sequence having at least 60% sequence identity to the amino acid sequence of SEQ ID NO 9. Glycolate dehydrogenase peptides may further comprise an amino acid sequence targeting the enzyme or subunits to the chloroplast or the chloroplast membrane or the nucleic acids encoding one or more polypeptides having glycolate dehydrogenase activity are integrated in the chloroplast genome of cells of the plant. Again, the one or more nucleic acid encoding one or more polypeptides having glycolate dehydrogenase activity may be operably linked to a plant-expressible promoter, such as the promoter region of the ribulose-bisphosphate carboxylasc small subunit gene of Oryza sativa or the promoter region of the cytosolic fructose 1,6 bisphosphatase gene of Oryza sativa and optionally to a 3′ end region involved in transcription termination and/or polyadenylation. The plant may be a monocotyledonous plant, a dicotyledonous plant or a gymnosperm, including wheat, rice, maize, sorghum, millet, rye, oats, sugarcane, cotton, sugarbeet, Brassica plants, including B. napus, B. oleracea, B. juncea, B. rapa or B. carinata.

In another embodiment, the invention provides plant cell, plant parts, plants or seed comprising nucleic acid encoding an enzyme comprising sedoheptulose 1,7-bisphosphatase activity; and one or more nucleic acids encoding one or more polypeptides having the enzymatic activity of glycolatc dehydrogenase, whcrcin the one or morc polypeptides are located in chloroplasts of the plants, as herein described.

In yet another embodiment, the invention relates to a food product obtained from a plant or plant part or seed a herein described.

The invention also provides the simultaneous use of a nucleic acid encoding an enzyme comprising sedoheptulose 1,7-bisphosphatase activity as herein described and one or more nucleic acids encoding one or more polypeptides having the enzymatic activity of glycolate dehydrogenase, as herein described, for improving the assimilation of carbon in a plant, or for increasing the growth rate or for improving biomass production of a plant.

DETAILED DESCRIPTION OF DIFFERENT EMBODIMENTS

As used herein “biomass” is the quantity of matter produced by individual plants, or by surface area on which the plants are grown. Several parameters may be measured in order to determine the increase of biomass production. Examples of such parameters are the height of the plant, surface of the leaf blade, shoot dry weight, root dry weight, seed number, seed weight, seed size, . . . . In that respect, seed production, or seed yield, is one specific indicator of biomass. Seed production or seed yield can be measured per individual plant or per surface area where the plants are grown. These parameters are generally measured after a determined period of growth in soil or at a specific step of growth, for example at the end of the vegetative period, and compared between plants transformed with one or more nucleic acids according to the invention and plants not transformed with such one or more nucleic acids.

The increase of carbon fixation by the plant can be determined by measuring gas exchange and chlorophyll fluorescence parameters. A convenient methodology, using the LI-6400 system (Li-Cor) and the software supplied by the manufacturer, is described in R. Kebeish et al., 2007, and is incorporated herein by reference.

The current invention is based on the observation that photosynthetic assimilation of carbon in plants can be further increased by simultaneously providing cells of such plants with increased RuBP regeneration capacity and providing the chloroplasts of such cells with glycolate catabolizing enzymes to bypass photorespiration.

Increasing the RuBP regeneration capacity can be conveniently achieved by inserting into the genome of a plant cell a nucleic acid encoding an enzyme comprising sedulose 1,7-bisphosphatase activity. Methods for expressing SBPase encoding enzymes in plants have been described e.g. in WO 00/70062 (herein incorporated by reference).

As used herein, “inserting” or “introducing” a nucleic acid into the plant genome or “providing” a nucleic acid to a plant cell should be understood as encompassing all methods available in the art to provide a plant cell comprising the nucleic acid, whether such introduction is achieved by plant transformation methods or by crossing the recipient plant with a donor plant into which the nucleic acid of interest was previously introduced. Other methods of transferring a nucleic acid from a donor plant to a recipient plants include e.g. protoplast fusion followed by regeneration of the fused protoplasts.

In the context of the present invention the term “genome” includes bot the nuclear genome as well as the plastid genome. It will be clear that the methods of transformation used are of minor relevance to the current invention. Transformation of plants is now a routine technique. Advantageously, any of several transformation methods may be used to introduce the nucleic acid/gene of interest into a suitable ancestor cell. Transformation methods include the use of liposomes, electroporation, chemicals that increase free DNA uptake, injection of the DNA directly into the plant, particle gun bombardment, transformation using viruses or pollen and microprojection. Methods may be selected from the calcium/polyethylene glycol method for protoplasts (Krens et al. (1982) Nature 296: 72-74; Negrutiu et al. (1987) Plant. Mol. Biol. 8: 363-373); electroporation of protoplasts (Shillito et al. (1985) Bio/Technol. 3: 1099-1102); microinjection into plant material (Crossway et al. (1986) Mol. Gen. Genet. 202: 179-185); DNA or RNA-coated particle bombardment (Klein et al. (1987) Nature 327: 70) infection with (non-integrative) viruses and the like. Transgenic plants can also be produced by using needle-like crystals (“whiskers”) of silicon carbide; viral-mediated transformation; Agrobacterium-, Rhizobium-, Mesorhizobium- and Sinorizobium-mediated transformation. See, for example, U.S. Pat. Nos. 5,405,765; 5,472,869; 5,538,877; 5,538,880; 5,550,318; 5,641,664; 5,736,369; 5,736369; and US patent applications 2005/0289672 and 2005/0289667.

In the case of canola or other Brassica oilseed rape plants, a suitable transformation method is that disclosed in De Block et al. (Plant Physiol. (1989) 91: 694-701).

Two major methods are currently used in soybean transformation. The first method utilizes particle bombardment, advantageously of embryogenic calluses, cell cultures on a solid or in suspension, or other proliferative embryogenic tissues (Trick & Finer, 1998; Maughan et al, 1999; Santarem & Finer, 1999; Droste et al, 2002), while the second method involves Agrobacterium-mediated transformation of organogenic tissue, such as cotyledonary node tissues (Zhang et al, 1999; Clemente et al, 2000; Olhoft & Somers, 2001; Olhoft et al, 2001), or tissue derived from mature soybean seeds (U.S. Pat. No. 7,473,822; EP10356036.3). WO2011/095460 also describes soybean transformation methods, using HPPD inhibitors as selective agents.

Agrobacterium-mediated transformation of cotton has been described e.g. in U.S. Pat. No. 5,004,863 or 6,483,013 and cotton transformation by particle bombardment is reported e.g. in WO 92/15675. Other suitable cotton transformation methods are disclosed e.g. in WO 00071733 and U.S. Pat. No. 5,159,135, which disclosures are incorporated by reference herein.

Agrobacterium mediated transformation of Triticum plants, including wheat has been described e.g. in WO2011/013764.

An efficient Agrobacterium-mediated transformation method for corn (maize), using pre-incubation of compact embryogenic calli in plant phenolic compounds has been described in WO 98/37212.

For rice, Agrobacterium-mediated transformation (Hiei et al., 1994, and Hiei et al., 1997, incorporated herein by reference), electroporation (U.S. Pat. Nos. 5,641,664 and 5,679,558, incorporated herein by reference), or bombardment (Christou et al., 1991, incorporated herein by reference) could be advantageously performed. A suitable technology for transformation of monocotyledonous plants, and particularly rice, is described in WO 92/09696.

Methods for chloroplast transformation are also well known in the art, including those described in WO02/055651, WO01/42441, WO02/057466, WO2004/015115, WO2004/016793, WO01/64023, WO01/81605, WO2004/005480, WO2004/035734, WO97/32977, WO00/39313, WO2010/061186, WO2006/072607, WO2007/031547, WO2008/003666, WO06/108830 or WO2010/079117.

Each of referenced transformation methods (nuclear or chloroplastic) is expressly incorporated herein by reference in entirety.

To achieve localization of nuclearly expressed polypeptides, such as SBPase polypeptide(s) and/or glycolate dehydrogenase polypeptide(s) in plastids of cells, particularly in chloroplasts of the plant cells, the coding region of the SBPase polypeptide(s) may be operably linked to a plastid targeting signal, particularly a chloroplast targeting signal. Thus, a nucleic acid which encodes a chloroplast transit peptide, such as a heterologous chloroplast transit peptide may be introduced at the 5′ end of the nucleic acid sequence encoding an SBPase or glycolate dehydrogenase polypeptide(s), with this transit peptide sequence being arranged between the promoter region and the nucleic acid encoding the SBPase or glycolate dehydrogenase polypeptide so as to permit expression of a transit peptide/SBPase or transit peptide/glycolate dehydrogenase fusion protein. The transit peptide allows to direct the fused protein into the plastids, more especially the chloroplasts, with the fusion protein being cleaved between the transit peptide and the polypeptide of interest when the latter enters the plastid. The transit peptide may be a single peptide, such as an EPSPS transit peptide (described in U.S. Pat. No. 5,188,642) or a transit peptide of the plant ribulose bisphosphate carboxylase/oxygenase small subunit (RuBisCO ssu), for example the chloroplast transit peptide derived from the ribulose-1,5-bisphosphate carboxylase small subunit gene from Solanum tuberosum (GenBank: G68077, amino acids 1-58), where appropriate including a few amino acids of the N-terminal part of the mature RuBisCO ssu (see e.g. EP 189 707), or the chloroplast targeting peptide derived from the potato rbcSl gene (gi21562). A transit peptide may be the whole naturally occurring (wild-type) transit peptide, a functional fragment thereof, or a functional mutant thereof. It can also be a chimeric transit peptide wherein at least two transit peptides are associated to each other or wherein parts of different transit peptides are associated to each other in a functional manner. One example of such chimeric transit peptide comprises a transit peptide of the sunflower RuBisCO ssu fused to the N-terminal part of the maize RuBisCO ssu, fused to the transit peptide of the maize RuBisCO ssu, as described in patent EP 508 909. The person skilled in the art will be able to construct nucleic acid suitable for performing the invention comprising a nucleic acid encoding a mature (i.e. without transit peptide) SBPase or glycolate dehydrogenase polypeptide, which may be optionally optimized for expression in particular crop plants, such as for expression in wheat, and wherein the first ATG codon, if any, may or may not be deleted, operably-linked to a chloroplast transit peptide.

For the purpose of expressing the nucleic acid(s) which encode the polypeptide(s) having the enzymatic activity as required for the present invention in plant cells, any convenient regulatory sequences can be used. The regulatory sequences will provide transcriptional and translational initiation as well as termination regions, where the transcriptional initiation may be constitutive or inducible. The coding region can be operably linked to such regulatory sequences. Suitable regulatory sequences are represented by the constitutive CaMV 35S promoter. Alternatively, the constitutive ubiquitin promoter can be used, in particular the maize ubiquitin promoter (GenBank: gi19700915). Examples for inducible promoters represent the light inducible promoters of the small subunit of RuBisCO and the promoters of the “light harvesting complex binding protein (Ihcb)”. Advantageously, the promoter region of the Gos2 gene of Oryza sativa including the 5′ UTR of the GOS2 gene with intron (de Pater et al., 1992), the promoter region of the RuBisCO small subunit gene of Oryza sativa (Kyozuka J. et al., 1993), or the promoter region of the actin 1 gene of Oryza sativa (McElroy D. et al., 1990) may be used. Other promoters which may be used include the promoter region of the RuBisCO small subunit gene of Oryza sativa as described by Nomura et al. 2000, Plant Molecular Biology 44(1) 99-106 or as represented in SEQ ID No 12 from nucleotide 2010-4759 (counter clockwise). Another suitable promoter is the promoter region of the cytolsolic fructose 1,6-bisphosphatase gene of Oryza sativa as described by Si et al. 2003 (Acta Botanica Sinica 45, 359-364) or as described in SEQ ID No 11 from nucleotide 75 to nucleotide 1272.

According to the invention, use may also be made, in combination with the promoter, of other regulatory sequences, which are located between the promoter and the coding sequence, such as transcription activators (“enhancers”), for instance the translation activator of the tobacco mosaic virus (TMV) described in Application WO 87/07644, or of the tobacco etch virus (TEV) described by Carrington & Freed 1990, for example, or introns such as the adh1 intron of maize or intron 1 of rice actin.

As a regulatory terminator or polyadenylation sequence, use may be made of any 3′ end region function in plant cells, such as for example the nos terminator of Agrobacterium tumefaciens, of viral origin, such as for example the CaMV 35S terminator, or of plant origin, such as for example a histone terminator as described in Application EP 0 633 317.

Sedoheptulose-bisphosphatase (also sedoheptulose-1,7-bisphosphatase or SBPase) (EC 3.1.3.37) is an enzyme that catalyzes the removal of a phosphate group from sedoheptulose 1,7-bisphosphate to produce sedoheptulose 7-phosphate. This enzyme is involved in the regeneration of 5-carbon sugars during the Calvin cycle. SBPase is activated in the presence of light through a ferredoxin/thioredoxin system. The reduced thioredoxin is used to reduced a cysteine-cysteine disulfide bond in SBPase to a dithiol, which converts the SBPase into its active form. Mg2+ concentration has a significant impact on the activity of SBPase and the rate of the reactions it catalyzes. SBPase is subject to negative feedback regulation by sedoheptulose-7-phosphate and inorganic phosphate, the products of the reaction it catalyzes. SBPase is found across many species, in addition to being universally present in photosynthetic organism.

SBPase activity can be determined by a phosphate release assay as described by Lefebvre et al. 2005 or as described with modifications by Liu et al. 2012 (Molecular Plant Advance pages 1-18).

A suitable SBPase coding region could be the coding sequence of the sedoheptulose 1,7-gene of Oryza sativa as described by Feng et al. 2007 (Plant Cell Reporter 26, 1635-1646) or as described in SEQ ID No 12 from nucleotide position 362 to 1540 (counterclock wise). Of course, any nucleotide sequence encoding a polypeptide having the amino acid sequence of SEQ ID No 2 can equally be used.

The nucleic acid molecules encoding SBPases as described herein may be modified, for example, by codon optimization to facilitate expression in heterologous cells. This type of modification changes or alters the nucleotide sequence that encodes a protein of interest to use, throughout the sequence, codons that are more-commonly used in the transgenic expression host cell. In addition, changes may be made to the nucleotide sequence that encodes the protein to adjust the relative concentration of A/T and G/C base pairs to ratios that are more similar to those of the expression host.

Moreover, numerous other SBPase polypeptide(s) are known in the art, and nucleic acid molecules encoding such other SBPase polypeptide(s) may also be used in the context of the current invention. The amino acid sequences of these SBPase polypeptide(s) can be found in publicly available databases and include the following: (Zea mays) Accession: NP_001148402.1/GI: 226506366; (Triticum aestivum) Accession: P46285.1/GI: 1173347; (Arabidopsis thaliana) Accession: P46283.1/GI: 1173345; (Spinacia oleracea) Accession: 020252.1/GI: 3914940; (Arabidopsis thaliana) Accession: AEE79443.1/GI: 332645922; (Arabidopsis thaliana) Accession: NP_191139.1/GI: 15228194; (chloroplast/Arabidopsis thaliana) Accession: AAB33001.1/GI: 786466; (Arabis alpina) Accession: KFK34828.1/GI: 674242063 (Oryza sativa Japonica Group) Accession: BAF14207.1/GI: 113563864; (Sorghum bicolor) Accession: EES01767.1/GI: 241928622; (Cucumis sativus) Accession: ACQ82818.1/GI: 229597543; (Oryza sativa Japonica Group) Accession: NP_001052293.1/GI: 115457386; (Sorghum bicolor) Accession: XP_002456647.1/GI: 242055003; (Solanum lycopersicum) Accession: NP_001234585.1/GI: 350538149; (Triticum aestivum) Accession: CDM85031.1/GI: 669030491; (Cucumis sativus) Accession: NP 001267658.1/GI: 525507240; (Morus alba var. multicaulis) Accession: ABK76304.1/GI: 118175929; (Solanum lycopersicum) Accession: ACR46521.1/GI: 238563983; (Marchantia polymorpha) Accession: ABF68592.1/GI: 99903817; (Triticum aestivum) Accession: CAA46507.1/GI: 14265; (Gossypium arboreum) Accession: KHG28838.1/GI: 728849395; (Brachypodium distachyon) Accession: XP_003564625.1/GI: 357125896; (Nelumbo nucifera) Accession: XP_010256671.1/GI: 720002409; (Morus notabilis) Accession: XP_010093027.1/GI: 703086526 (Eucalyptus grandis) Accession: XP_010031259.1/GI: 702473166; (Nicotiana sylvestris) Accession: XP_009776701.1 GI: 698578260; (Nicotiana sylvestris) Accession: XP_009780073.1/GI: 698454000; (Nicotiana tomentosiformis) Accession: XP_009622770.1/GI: 697137344; (Nicotiana tomentosiformis) Accession: XP_009590435.1/GI: 697100291; (Musa acuminata subsp. malaccensis) Accession: XP_009398831.1/GI: 694997329; (Pyrus×bretschneideri) Accession: XP_009341715.1/GI: 694428277; (Pyrus×bretschneideri) Accession: XP_009361829.1/GI: 694366289; (Pyrus×bretschneideri) Accession: XP_009351012.1/GI: 694320367; (Pyrus×bretschneideri) Accession: XP_009351006.1/GI: 694320364; (Pyrus×bretschneideri) Accession: XP_009344459.1 GI: 694318830; (Brassica rapa) Accession: XP_009116278.1/GI: 685364140; (Brassica rapa) Accession: XP_009103972.1/GI: 685332510; (Brassica rapa) Accession: XP_009103971.1/GI: 685332508; (Brassica rapa) Accession: XP_009139137.1/GI: 685296600; (Brassica rapa) Accession: XP_009139136.1/GI: 685296598; (Brassica rapa) Accession: XP_009139135.1/GI: 685296596; (Nicotiana tabacum) Accession: A1199841.1/GI: 671775216; (Phoenix dactylifera) Accession: XP_008775070.1/GI: 672189771; (Phoenix dactylifera) Accession: XP_008785431.1/GI: 672124109; (Theobroma cacao) Accession: XP_007009096.1/GI: 590562472; (Cucumis melo) Accession: XP_008463213.1/GI: 659126497; (Malus domestica) Accession: XP 008383089.1/GI: 657982110; (Malus domestica) Accession: XP_008372025.1/GI: 657960888; (Malus domestica) Accession: XP_008381958.1/GI: 657945883; (Medicago truncatula) Accession: AES71104.1/GI: 355489901; (Prunus mume) Accession: XP_008234053.1/GI: 645256667; (Prunus mume) Accession: XP_008226111.1/GI: 645239407; (Theobroma cacao) Accession: EOY17906.1/GI: 508726009; (Brassica rapa subsp. chinensis) Accession: AHY18974.1/GI: 619835283; (Morus notabilis) Accession: EXB53355.1/GI: 587863593; (Oryza brachyantha) Accession: XP_006664812.1/GI: 573965402; (Glycine max) Accession: XP_003552524.1/GI: 356568652; (Glycine max) Accession: XP_003538446.1/GI: 356539931; (Populus trichocarpa) Accession: XP_002316235.1/GI: 224112589; (Citrus sinensis) Accession: XP_006486375.1/GI: 568866045; (Solanum tuberosum) Accession: XP_006355654.1/GI: 565378420; (Populus trichocarpa) Accession: EEF02406.1/GI: 222865275; (Genlisea aurea) Accession: EPS64973.1/GI: 527195911; (Setaria italica) Accession: XP_004970606.1/GI: 514784500; (Cicer arietinum) Accession: XP_004503228.1/GI: 502137914; (Aegilops tauschii) Accession: EMT17623.1/GI: 475576723; (Triticum urartu) Accession: EMS67709.1/GI: 474425491; (Fragaria vesca subsp. vesca) Accession: XP_004307611.1/GI: 470143913; (Fragaria vesca subsp. vesca) Accession: XP_004294065.1/GI: 470115771; (Arabidopsis lyrata subsp. lyrata) Accession: EFH52595.1/GI: 297322174; (Ricinus communis) Accession: EEF31985.1/GI: 223530064; (Vitis vinifera) Accession: XP_002263049.1/GI: 225466690; (Medicago truncatula) Accession: XP_003600853.1/GI: 357461143; (Arabidopsis thaliana) Accession: AAM91137.1/GI: 22136118; (Triticum aestivum) Accession: CBH32512.1/GI: 300681420; (Spinacia oleracea) Accession: AAB81104.1/GI: 2529376; (Arabidopsis lyrata subsp. lyrata) Accession: XP_002876336.1/GI: 297816906; (Oryza sativa Indica Group) Accession: AA022559.1/GI: 27804772 (Oryza sativa Indica Group) Accession: AA022558.1/GI: 27804768 (Arabidopsis thaliana) Accession: AAK96860.1/GI: 15451178 (Ricinus communis) Accession: XP_002530415.1/GI: 255579134 (Zea mays) Accession: ACG31345.1/GI: 195619030 (Marchantia polymorpha) Accession: ABK00060.1/GI: 116582760 (Arabidopsis thaliana) Accession: CAB81605.1/GI: 7263568.

SBPases from green algae may also be used and include amino acid sequences as represented by (Chlamydomonas reinhardtii) Accession: P46284.1/GI: 1173346; (Chlorella variabilis) Accession: XP_005850770.1/GI: 552843189; (Chlamydomonas reinhardtii) Accession: CAA52439.1/GI: 515618; (Chlamydomonas reinhardtii) Accession: EDP04487.1/GI: 158278724; (Chlamydomonas reinhardtii) Accession: XP_001691997.1/GI: 159467635; (Micromonas sp. RCC299) Accession: ACO64947.1/GI: 226518956; (Auxenochlorella protothecoides) Accession: KFM29064.1/GI: 675356624; (Bathycoccus prasinos); Accession: XP_007512235.1/GI: 612393430; (Coccomyxa subellipsoidea C-169) Accession: XP_005642742.1/GI: 545353814; (Ostreococcus tauri) Accession: XP_003078456.1/GI: 308802285; (Bathycoccus prasinos) Accession: CC066323.1/GI: 424513701; (Volvox carteri f. nagariensis) Accession: EFJ52154.1/GI: 300267972; (Volvox carteri f. nagariensis) Accession: XP_002946928.1/GI: 302830724; (Micromonas sp. RCC299) Accession: XP_002503689.1/GI: 255080218; (Chlamydomonas reinhardtii) Accession: CAA74960.1/GI: 2342564; (Chlamydomonas sp. W80) Accession: BAA94305.1/GI: 7544134.

It could also be advantageous to use SBPases comprised in a polypeptide having multiple enzymatic activities such as Fructose-1,6-bisphosphatase class 1/Sedoheputulose-1,7-bisphosphatase (Ostreococcus tauri) Accession: CEF97347.1/GI: 693500974 Fructose-1,6-bisphosphatase class 1/Sedoheputulose-1,7-bisphosphatase (Ostreococcus tauri) Accession: CEF97908.1/GI: 693499856 Fructose-1,6-bisphosphatase class 1/Sedoheputulose-1,7-bisphosphatase (Ostreococcus tauri) Accession: CEF98908.1/GI: 693498312 Fructose-1,6-bisphosphatase class I/Sedoheputulose-1,7-bisphosphatase (Ostreococcus tauri) Accession: CEG02024.1/GI: 693497067 Fructose-1,6-bisphosphatase class 1/Sedoheputulose-1,7-bisphosphatase (Ostreococcus tauri) Accession: CAL52685.1/GI: 116056396.

The invention may also use variants of the SBPase polypeptide(s) herein described. Variants include, but are not limited to, deletions, additions, substitutions, insertions of e.g. 1 to 20 amino acids, such as 1 to 10, or 1 to 5). The term “variant” is intended to mean substantially similar sequences. Naturally occurring allelic variants can be identified with the use of well-known molecular biology techniques, as, for example, with polymerase chain reaction (PCR) and hybridization techniques as herein outlined. Variant (nucleotide) sequences also include synthetically derived (nucleotide) sequences, such as those generated, for example, by using site-directed mutagenesis. Generally, amino acid sequence variants of SBPase polypeptide(s) described herein will have at least 60%, to 70%, e.g., preferably 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, to 79%, generally at least 80%, e.g., 81% to 84%, at least 85%, e.g., 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, to 98% and 99% sequence identity to the amino acid sequences of the SBPases explicitly described herein, and will retain SBPase activity. Variant SBPase polypeptide(s) may have at least 60% sequence identity to the amino acid sequence of SEQ ID No 2. Variant SBPase polypeptide(s) may have at least 95% sequence identity to the amino acid sequence of SEQ ID No 2. Generally, nucleotide sequence variants have at least 60%, to 70%, e.g., preferably 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, to 79%, generally at least 80%, e.g., 81% to 84%, at least 85%, e.g., 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, to 98% and 99% sequence identity to the nucleotide sequences encoding the SBPases described herein, and the encoded products retain SBPase activity. Variant nucleic acids encoding SBPase polypeptide(s) may have at least 60% sequence identity to the nucleotide sequence of SEQ ID No 1. Variant nucleic acids encoding SBPases may have at least 95% sequence identity to the nucleotide sequence of SEQ ID No 1.

For the purpose of this invention, the “sequence identity” of two related nucleotide or amino acid sequences, expressed as a percentage, refers to the number of positions in the two optimally aligned sequences which have identical residues (×100) divided by the number of positions compared. A gap, i.e., a position in an alignment where a residue is present in one sequence but not in the other, is regarded as a position with non-identical residues. The “optimal alignment” of two sequences is found by aligning the two sequences over the entire length according to the Needleman and Wunsch global alignment algorithm (Needleman and Wunsch, 1970, J Mol Biol 48(3):443-53) in The European Molecular Biology Open Software Suite (EMBOSS, Rice et al., 2000, Trends in Genetics 16(6): 276-277; see e.g. http://www.ebi.ac.uk/emboss/align/index.html) using default settings (gap opening penalty=10 (for nucleotides)/10 (for proteins) and gap extension penalty=0.5 (for nucleotides)/0.5 (for proteins)). For nucleotides the default scoring matrix used is EDNAFULL and for proteins the default scoring matrix is EBLOSUM62.

Variant SBPase encoding nucleic acids may be identified by hybridization. “Stringent hybridization conditions” can be used to identify nucleotide sequences, which are substantially identical to a given nucleotide sequence. Stringent conditions are sequence dependent and will be different in different circumstances. Generally, stringent conditions are selected to be about 5° C. lower than the thermal melting point (Tm) for the specific sequences at a defined ionic strength and pH. The Tm is the temperature (under defined ionic strength and pH) at which 50% of the target sequence hybridizes to a perfectly matched probe. Typically stringent conditions will be chosen in which the salt concentration is about 0.02 molar at pH 7 and the temperature is at least 60° C. Lowering the salt concentration and/or increasing the temperature increases stringency. Stringent conditions for RNA-DNA hybridizations (Northern blots using a probe of e.g. 100 nt) are for example those which include at least one wash in 0.2×SSC at 63° C. for 20 min, or equivalent conditions. “High stringency conditions” can be provided, for example, by hybridization at 65° C. in an aqueous solution containing 6×SSC (20×SSC contains 3.0 M NaCl, 0.3 M Na-citrate, pH 7.0), 5×Denhardt's (100×Denhardt's contains 2% Ficoll, 2% Polyvinyl pyrollidone, 2% Bovine Serum Albumin), 0.5% sodium dodecyl sulphate (SDS), and 20 μg/ml denaturated carrier DNA (single-stranded fish sperm DNA, with an average length of 120-3000 nucleotides) as non-specific competitor. Following hybridization, high stringency washing may be done in several steps, with a final wash (about 30 min) at the hybridization temperature in 0.2-0.1×SSC, 0.1% SDS. “Moderate stringency conditions” refers to conditions equivalent to hybridization in the above described solution but at about 60-62° C. Moderate stringency washing may be done at the hybridization temperature in 1×SSC, 0.1% SDS. “Low stringency” refers to conditions equivalent to hybridization in the above described solution at about 50-52° C. Low stringency washing may be done at the hybridization temperature in 2×SSC, 0.1% SDS. See also Sambrook et al. (1989) and Sambrook and Russell (2001).

The invention also requires the presence of one or more peptides having glycolate dehydrogenase activity, within the chloroplasts of the plant cells, in addition to increased SBPase activity.

glycolate dehydrogenase (EC 1.1.99.14) is an enzyme that catalyzes the chemical reaction:

glycolate+acceptorglyoxylate+reduced acceptor

The enzymatic activity of glycolate dehydrogenases can be defined by the oxidation of glycolate to form glyoxylate using organic cofactors, whereas glycolate oxidases, present for example in plant peroxisomes, use molecular oxygen as a cofactor and release hydrogen peroxide. Such clear distinction between glycolate dehydrogenases and glycolate oxidases based on the nature of the cofactors has not always been done, and as an example the E. coli glycolate dehydrogenase encoded by the glc operon was previously named glycolate oxidase (Bari et al., 2004, J. of Experimental Botany, Vol 55, N^o 397, 623-630). The glycolate dehydrogenase activity can be assayed according to Lord J. M. 1972 Biochim. Biophys. Acta 267, 227-237. Alternatively, complementation analysis with mutants of E. coli deficient in the three subunits forming active endogenous glycolate dehydrogenase may be performed. These mutants of E. coli are incapable of growing on glycolate as the sole carbon source. When the overexpression of an enzyme in these deficient mutants restores the growth of the bacteria on the medium containing glycolate as the sole carbon source, it means that this enzyme encodes a functional equivalent to the E. coli glycolate dehydrogenase. The method and means for the complementation analysis is described in Bari et al, 2004, and incorporated herein by reference. Polypeptides having the enzymatic activity of a glycolate dehydrogenase, and nucleic acids encoding them, have been identified from various sources, including bacteria, algae, and plants.

A suitable glycolate dehydrogenase coding region could be the glycolate dehydrogenase coding region of Chlamydomonas as described e.g. in SEQ ID No 10 from nucleotide 3666 to 6485. Again, any nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID No 6, or a truncated form thereof comprising the amino acid sequence of SEQ ID No 7, could be equally used.

Other suitable polypeptides having the enzymatic activity of a glycolate dehydrogenase are those encoded by the E. coli glc operon such as polypeptides which comprise the amino acid sequences of SEQ ID NOs: 3 (GlcD), 4 (GlcE) and 5 (GlcF).

Polypeptide(s) having the enzymatic activity of a glycolate dehydrogenase and derived from Arabidopsis thaliana or other higher plant sources may also be used.

The glycolate dehydrogenase activity involved in the method of the invention may be obtained by one or more polypeptides. When said activity is obtained through more than one polypeptides, the nucleic acids encoding the polypeptides may be transferred to plant cells in a single plasmid construct or independently in several constructs. The polypeptides corresponding to GlcD, E and F of the glc operon may also be provided as a fusion protein, as described in WO2011/095528.

The glycolate dehydrogenase may also be a chimeric glycolate dehydrogenase. The term “chimeric glycolate dehydrogenase” is intended to mean a glycolate dehydrogenase which is obtained by combining portions of enzymes from various origins, such as example the N-terminal portion of a first enzyme with the C-terminal portion of a second enzyme, so as to obtain a novel functional chimeric glycolate dehydrogenase, with each portion selected for its particular properties.

Numerous glycolate dehydrogenase polypeptides are known in the art from green plants including algae and may also be used in the context of the invention. The amino acid sequences of these glycolate dehydrogenase polypeptide(s) can be found in publicly available databases and include the following: (Chlamydomonas reinhardtii) Accession: EDP01639.1/GI: 158275864; (Chlamydomonas reinhardtii) Accession: XP_001695381.1/GI: 159474536; (Chlamydomonas reinhardtii) Accession: ABG36932.1/GI: 109659947; (Micromonas sp. RCC299) Accession: ACO67704.1/GI: 226521718; (Micromonas sp. RCC299) Accession: XP_002506446.1/GI: 255089048 (Tetraselmis sp. GSL018) Accession: JAC82740.1/GI: 654187807; (Tetraselmis sp. GSL018) Accession: JAC77648.1/GI: 654171420; (Tetraselmis sp. GSL018) Accession: JAC73922.1/GI: 654156881; (Tetraselmis sp. GSL018) Accession: JAC70698.1/GI: 654143314; (Tetraselmis sp. GSL018) Accession: JAC65595.1/GI: 654119087; (Tetraselmis sp. GSL018) Accession: JAC65376.1/GI: 654117941; (Coccomyxa subellipsoidea C-169) Accession: XP_005648725.1/GI: 545367841; (Coccomyxa subellipsoidea C-169) Accession: EIE24181.1/GI: 384250702; (Arabidopsis thaliana) Accession: Q9LSV0.1/GI: 75274028; (Arabidopsis thaliana) Accession: Q94AX4.1/GI: 75165032; (Arabidopsis thaliana) Accession: AEE84032.1/GI: 332658632; (Arabidopsis thaliana) Accession: AEE84031.1/GI: 332658631; (Arabidopsis thaliana) Accession: NP_001078406.1/GI: 145333373; (Arabidopsis thaliana) Accession: NP_193570.1/GI: 15236857; (Micromonas sp. RCC299) Accession: ACO66182.1/GI: 226520193; (Genlisea aurea) Accession: EPS70695.1/GI: 527204561; (Chlamydomonas reinhardtii) Accession: EDP06163.1/GI: 158280405; (Micromonas sp. RCC299) Accession: XP_002504924.1/GI: 255084986; (Chlamydomonas reinhardtii) Accession: XP_001703481.1/GI: 159491040; (Micromonas sp. RCC299) Accession: ACO61928.1/GI: 226515933; (Chlamydomonas reinhardtii) Accession: BAK61668.1/GI: 343403745; (Micromonas pusilla CCMP1545) Accession: EEH55456.1/GI: 226458158; (Micromonas pusilla CCMP1545) Accession: EEH51246.1/GI: 226453939; (Micromonas pusilla CCMP1545) Accession: XP_003064341.1/GI: 303290108; (Micromonas pusilla CCMP1545) Accession: XP_003060687.1/GI: 303282791; (Micromonas sp. RCC299) Accession: XP_002500670.1/GI: 255073991; (Coccomyxa subellipsoidea C-169) Accession: XP_005649475.1/GI: 545369460; (Spinacia oleracea) Accession: P05414.1/GI: 121530; (Oryza sativa Japonica Group) Accession: Q10CE4.1/GI: 122246745; (Arabidopsis thaliana) Accession: Q24JJ8.1/GI: 122195548; (Arabidopsis thaliana) Accession: Q9LJH5.1/GI: 75335069; (Oryza sativa Japonica Group) Accession: Q8H3I4.2/GI: 75329161; (Oryza sativa Japonica Group); Accession: Q7XPR4.3/GI: 317376216; (Oryza sativa Indica Group) Accession: B8B8K5.2/GI: 317376202; (Oryza sativa Japonica Group) Accession: Q7FAS1.1/GI: 75326731; (Oryza sativa Japonica Group) Accession: Q6YT73.1/GI: 75325236; (Arabidopsis thaliana) Accession: 049506.1/GI: 75318383; (Arabidopsis thaliana) Accession: Q9LRS0.1/GI: 13124263; (Arabidopsis thaliana) Accession: Q9LRR9.1/GI: 13124262; (Arabidopsis thaliana) Accession: F41907.1/GI: 449061823; (Oryza sativa Indica Group) Accession: Q01KC2.2/GI: 317376213; (Oryza sativa Indica Group) Accession: B8AUI3.1/GI: 317376201; (Oryza sativa Indica Group) Accession: B8B7C5.1/GI: 317376200; (Oryza sativa Indica Group) Accession: B8AKX6.1/GI: 317376187; (Arabidopsis thaliana) Accession: AEE75523.1/GI: 332642002; (Arabidopsis thaliana) Accession: AEE75522.1/GI: 332642001; (Arabidopsis thaliana) Accession: NP_001078155.1/GI: 145332397; (Arabidopsis thaliana) Accession: NP_001078154.1/GI: 145332395; (Arabidopsis thaliana) Accession: Q9CA90.1/GI: 75308938; (Arabidopsis thaliana) Accession: Q9LE33.1/GI: 75311082; (Arabidopsis thaliana) Accession: AEE75521.1/GI: 332642000; (Arabidopsis thaliana) Accession: AEE75520.1/GI: 332641999; (Arabidopsis thaliana) Accession: AEE75519.1/GI: 332641998; (Arabidopsis thaliana) Accession: AEE75518.1/GI: 332641997; (Arabidopsis thaliana) Accession: AEE75517.1/GI: 332641996.

Glycolate dehydrogenase polypeptide(s) from bacterial origin suitable for the invention include: (Vibrio ponticus) Accession: GAK83771.1/GI: 678239733; (Vibrio ponticus) Accession: GAK83770.1/GI: 678239732; (Vibrio ponticus) Accession: GAK83769.1/GI: 678239731; (Collimonas arenae) Accession: AIY39978.1/GI: 725876232; (Collimonas arenae) Accession: AIY39977.1/GI: 725876231; (Collimonas arenae) Accession: AIY39976.1/GI: 725876230; (Collimonas arenae) Accession: AIY39211.1/GI: 725875465; (Microcystis aeruginosa NIES-44) Accession: GAL92683.1/GI: 718250409; (Microcystis aeruginosa NIES-44) Accession: GAL93127.1/GI: 718250015; (Microcystis aeruginosa NIES-44) Accession: GAL93126.1/GI: 718250014; (Thermus filiformis) Accession: KGQ21896.1/GI: 702223516; (Thermus Aformis) Accession: KGQ21895.1/GI: 702223515; (Thermus filiformis) Accession: KGQ20890.1/GI: 702221593; (Escherichia coli) Accession: KGI49957.1/GI: 693353475; (Escherichia coli) Accession: KGI48634.1/GI: 693352107; (Escherichia coli) Accession: KGI48633.1/GI: 693352106; (Escherichia coli) Accession: KGI48632.1/GI: 693352105; (Agrobacterium tumefaciens LBA4213 (Ach5)) Accession: AHK00528.1/GI: 586948739; (Agrobacterium tumefaciens LBA4213 (Ach5)) Accession: AHK00527.1/GI: 586948738; (Agrobacterium tumefaciens LBA4213 (Ach5)) Accession: AHK00526.1/GI: 586948737; (Prochlorococcus sp. MIT 0703) Accession: KGG35322.1/GI: 691712480; (Prochlorococcus sp. MIT 0703) Accession: KGG34948.1/GI: 691712100; (Prochlorococcus sp. MIT 0702) Accession: KGG30233.1/GI: 691707154; (Prochlorococcus sp. MIT 0702) Accession: KGG29260.1/GI: 691706122; (Prochlorococcus sp. MIT 0701) Accession: KGG26677.1/GI: 691703415; (Prochlorococcus sp. MIT 0701) Accession: KGG25239.1/GI: 691701913; (Fimbriimonas ginsengisoli Gsoil 348) Accession: AIE85110.1/GI: 663072955; (Enhygromyxa salina) Accession: KFE73152.1/GI: 670626816; (Hyalangiutn minututn) Accession: KFE66519.1/GI: 670619991; (Bacillus clausii) Accession: KFE65667.1/GI: 670619134; (Bacillus clausii) Accession: KFE65666.1/GI: 670619133; (Bacillus clausii) Accession: KFE65665.1/GI: 670619132; (Bacillus clausii) Accession: KFE61464.1/GI: 670614876; (Streptomyces) Accession: WP_015578691.1/GI: 505391589; (Paenibacillus pini JCM 16418) Accession: GAF08561.1/GI: 585396797; (Paenibacillus pini JCM 16418) Accession: GAF08560.1/GI: 585396796; (Chondromyces apiculatus DSM 436) Accession: EYF06953.1/GI: 599568966; (Bacillus wakoensis JCM 9140) Accession: GAE27103.1/GI: 568807375; (Bacillus wakoensis JCM 9140) Accession: GAE27102.1/GI: 568807374; (Collimonas fungivorans Ter331) Accession: AEK60573.1/GI: 340551198; (Pseudomonas aeruginosa PAIR) Accession: AHA25009.1/GI: 557712983; (Pseudomonas aeruginosa PAIR) Accession: AHA25008.1/GI: 557712982; (Pseudomonas aeruginosa PAIR) Accession: AHA25007.1/GI: 557712981; (Pseudomonas aeruginosa PA1) Accession: AHA19210.1/GI: 557707183; (Pseudomonas aeruginosa PA1) Accession: AHA19209.1/GI: 557707182; (Pseudomonas aeruginosa PA1) Accession: AHA19208.1/GI: 557707181; (Ralstonia solanacearum FQY_4) Accession: AGH85082.1/GI: 469774892; (Ralstonia solanacearum FQY_4) Accession: AGH85081.1/GI: 469774891; (Ralstonia solanacearum FQY_4) Accession: AGH85080.1/GI: 469774890; (Bacillus cereus F837/76) Accession: AEW54435.1/GI: 364511036; (Streptomyces sp. PAMC26508) Accession: AGJ57220.1/GI: 478748640; (Ralstonia solanacearum FQY_4) Accession: YP_007633582.1/GI: 525970908; (Ralstonia solanacearum FQY_4) Accession: YP_007633581.1/GI: 525970907; (Ralstonia solanacearum FQY_4) Accession: YP_007633580.1/GI: 525970906; (Pseudomonas aeruginosa PA1) Accession: YP_008812253.1/GI: 558675545; (Pseudomonas aeruginosa PA1) Accession: YP_008812252.1/GI: 558675544; (Pseudomonas aeruginosa PA1) Accession: YP_008812251.1/GI: 558675543; (Pseudomonas aeruginosa PAIR) Accession: YP_008805904.1/GI: 558669194; (Pseudomonas aeruginosa PAIR) Accession: YP_008805903.1/GI: 558669193; (Pseudomonas aeruginosa PAIR) Accession: YP_008805902.1/GI: 558669192; (Achromobacter xylosoxidans NH44784-1996) Accession: YP_008027298.1/GI: 528981981; (Achromobacter xylosoxidans NH44784-1996) Accession: YP_008027297.1/GI: 528981980; (Achromobacter xylosoxidans NH44784-1996) Accession: YP_008027296.1/GI: 528981979; (Achromobacter xylosoxidans) Accession: WP_020924479.1/GI: 529016433; (Achromobacter xylosoxidans) Accession: WP_020924478.1/GI: 529016432; (Cystobacter fuscus DSM 2262) Accession: EPX58458.1/GI: 528053654; (Achromobacter xylosoxidans NH44784-1996) Accession: CCH04176.1/GI: 507098589; (Achromobacter xylosoxidans NH44784-1996) Accession: CCH04175.1/GI: 507098588; (Achromobacter xylosoxidans NH44784-1996) Accession: CCH04174.1/GI: 507098587; (Ralstonia solanacearum) Accession: WP_020832615.1/GI: 525966975; (Bacillus cereus F837/76) Accession: YP_005117948.1/GI: 376265236; (Streptomyces sp. PAMC26508) Accession: YP_007861704.1/GI: 479321653; (Collimonas fungivorans Ter331) Accession: YP_004751396.1/GI: 340785931; (Methylacidiphilum fumariolicum) Accession: WP_009058940.1/GI: 496349762; (Methylacidiphilum fumariolicum) Accession: WP_009058939.1/GI: 496349761; (Methylacidiphilum infernorum) Accession: WP_012463234.1/GI: 501439785; (Methylacidiphilum infernorum) Accession: WP_012463233.1/GI: 501439784; (Escherichia coli) Accession: WP_001703443.1/GI: 487497857; (Escherichia coli Nissle 1917) Accession: CCQ05977.1/GI: 441713650; Glycolate dehydrogenasE—Escherichia coli Nissle 1917) Accession: CCQ05976.1/GI: 441713649; (Escherichia coli Nissle 1917) Accession: CCQ05975.1/GI: 441713648; (Escherichia coli O10:K5(L):H4 str. ATCC 23506) Accession: CCP96322.1/GI: 441607314; (Escherichia coli O10:K5(L):H4 str. ATCC 23506) Accession: CCP96321.1/GI: 441607313; (Escherichia coli O10:K5(L):H4 str. ATCC 23506) Accession: CCP96320.1/GI: 441607312; (Escherichia coli O5:K4(L):H4 str. ATCC 23502) Accession: CCQ00751.1/GI: 441654202; (Escherichia coli O5:K4(L):H4 str. ATCC 23502) Accession: CCQ00750.1/GI: 441654201; (Escherichia coli O5:K4(L):H4 str. ATCC 23502) Accession: CCQ00749.1/GI: 441654200; (Bradyrhizobiaceae bacterium SG-6C) Accession: EGP10038.1/GI: 338234927; (Bradyrhizobiaceae bacterium SG-6C) Accession: EGP10037.1/GI: 338234926; (Bradyrhizobiaceae bacterium SG-6C) Accession: EGP10036.1/GI: 338234925; (Bradyrhizobiaceae bacterium SG-6C) Accession: EGP06191.1/GI: 338231050; (Oxalobacteraceae bacterium IMCC9480) Accession: EGF33327.1/GI: 327548681; (Oxalobacteraceae bacterium IMCC9480) Accession: EGF33326.1/GI: 327548680; (subunit GlcD—Yersinia ruckeri) Accession: CEK27678.1/GI: 731155805; (subunit GlcD—Photobacterium aphoticum) Accession: GAL06589.1/GI: 684144303; subunit GlcD—Citrobacter sp. CIP 55.13) Accession: CEJ67199.1/GI: 729037724; (subunit GlcD—Pseudomonas sp. SHC52) Accession: CDF93952.1/GI: 727047302; (subunit GlcE—Pseudomonas sp. SHC52) Accession: CDF93951.1/GI: 727047301; (subunit GlcF—Pseudomonas sp. SHC52) Accession: CDF93950.1/GI: 727047300; (subunit GlcD—Sulfitobacter sp. MM-124) Accession: KHA53862.1/GI: 722017351; (subunit GlcE—Sulfitobacter sp. MM-124) Accession: KHA53861.1/GI: 722017350; (subunit GlcF—Sulfitobacter sp. MM-124) Accession: KHA53859.1/GI: 722017348; (subunit GlcF—Bacillus weihenstephanensis) Accession: AIW86323.1/GI: 719645909; (subunit GlcD—Vibrio variabilis) Accession: GAL28777.1/GI: 684619748; (subunit GlcD—Vibrio variabilis) Accession: GAL28776.1/GI: 684619747; (subunit GlcD—Vibrio maritimus) Accession: GAL21031.1/GI: 684612272; (subunit GlcD—Vibrio maritimus) Accession: GAL21030.1/GI: 684612271; (subunit GlcD—Vibrio maritimus) Accession: GAL34207.1/GI: 684599410; (subunit GlcD—Vibrio maritimus) Accession: GAL34206.1/GI: 684599409; (subunit GlcD—Bacillus anthracis) Accession: AIK07381.1/GI: 672925963; (subunit GlcF—Bacillus anthracis) Accession: AIK07380.1/GI: 672925962; (subunit GlcE—Polaromonas sp. CG9_12) Accession: CDS52647.1/GI: 669784830; GlcF—Polaromonas sp. CG9_12) Accession: CDS52645.1/GI: 669784828; (subunit GlcD—Polaromonas sp. CG9_12) Accession: CDS52225.1/GI: 669784599; (subunit GlcD—Jejuia pallidilutea) Accession: GAL90108.1/GI: 697984292; (subunit GlcD—Bacillus cereus) Accession: AIE78640.1/GI: 663078473; (subunit GlcF—Bacillus cereus) Accession: AIE78639.1/GI: 663078472; (subunit GlcD—Algibacter lectus) Accession: GAL77539.1/GI: 693575543; (subunit GlcD—Algibacter lectus) Accession: GAL64672.1/GI: 693566108; (subunit GlcD—Jejuia pallidilutea) Accession: GAL70943.1/GI: 693563750; (subunit GlcD—Jejuia pallidilutea) Accession: GAL70942.1/GI: 693563749; (subunit GlcD—Jejuia pallidilutea) Accession: GAL67396.1/GI: 693559113; (subunit GlcD—Jejuia pallidilutea) Accession: GAL67395.1/GI: 693559112; (subunit GlcD—Lactobacillus plantarum CMPG5300) Accession: KGH44264.1/GI: 692529015; (subunit GlcD—Escherichia coli O145:H28 str. RM12581) Accession: AHY72347.1/GI: 628078821; GlcE—Escherichia coli O145:H28 str. RM12581) Accession: AHY72346.1/GI: 628078820; (subunit GlcF—Escherichia coli O145:H28 str. RM12581) Accession: AHY72345.1/GI: 628078819; (subunit GlcD—Escherichia coli O145:H28 str. RM12761) Accession: AHY66695.1/GI: 628030228; (subunit GlcE—Escherichia coli O145:H28 str. RM12761) Accession: AHY66694.1/GI: 628030227; (subunit GlcF—Escherichia coli O145:H28 str. RM12761) Accession: AHY66693.1/GI: 628030226; (subunit GlcD—Gilliamella apicola) Accession: AHN26357.1/GI: 597811526; (subunit GlcF—Roseibacterium elongatum DSM 19469) Accession: AHM04004.1/GI: 594548985; (subunit GlcE—Roseibacterium elongatum DSM 19469) Accession: AHM04003.1/GI: 594548984; (subunit GlcD—Roseibacterium elongatum DSM 19469) Accession: AHM04002.1/GI: 594548983; (subunit GlcF—Castellaniella defragrans 65Phen) Accession: CDM23960.1/GI: 589266024; (subunit GlcE—Castellaniella defragrans 65Phen) Accession: CDM23959.1/GI: 589266023; (subunit GlcD—Castellaniella defragrans 65Phen) Accession: CDM23958.1/GI: 589266022; (subunit GlcD—Bacillus subtilis E1) Accession: CCU59369.1/GI: 659925034; (subunit GlcF—Methylobacterium oryzae CBMB20) Accession: AIQ93092.1/GI: 689277013; (subunit GlcD—Burkholderia glathei) Accession: CDY76859.1/GI: 678299913; (subunit GlcD—Burkholderia glathei) Accession: CDY77463.1/GI: 678299256; (subunit GlcE—Burkholderia glathei) Accession: CDY77462.1/GI: 678299255; (subunit GlcF—Burkholderia glathei) Accession: CDY77461.1/GI: 678299254; (subunit GlcF—Paenibacillus sp. JCM 10914) Accession: GAE09049.1/GI: 560941948; (subunit GlcF—Paenibacillus sp. JCM 10914) Accession: GAE09047.1/GI: 560941946; (subunit GlcD—Paenibacillus sp. JCM 10914) Accession: GAE09046.1/GI: 560941945; (subunit GlcD—Paenibacillus sp. JCM 10914) Accession: GAE09045.1/GI: 560941944; (subunit GlcE—Synechocystis sp. PCC 6714) Accession: AIE75713.1/GI: 662705738; (subunit GlcF—Synechocystis sp. PCC 6714) Accession: AIE75280.1/GI: 662705305; (subunit GlcD—Synechocystis sp. PCC 6714) Accession: AIE74016.1/GI: 662704041; (Bacillus clausii) Accession: KFE61465.1/GI: 670614877; (subunit GlcE—Thioclava sp. 13D2W-2) Accession: KFE36767.1/GI: 669601292; (subunit GlcD—Thioclava sp. 13D2W-2) Accession: KFE36766.1/GI: 669601291; (subunit GlcF—Escherichia coli DSM 30083=JCM 1649=ATCC 11775) Accession: KFB96207.1/GI: 668710444; (subunit GlcE—Escherichia coli DSM 30083=JCM 1649=ATCC 11775) Accession: KFB96206.1/GI: 668710443; (subunit GlcD—Escherichia coli DSM 30083=JCM 1649=ATCC 11775) Accession: KFB96205.1/GI: 668710442; (subunit GlcF—Brucella suis bv. 2) Accession: AIB28969.1/GI: 648132977; (subunit GlcE—Brucella suis bv. 2) Accession: AIB28968.1/GI: 648132976; (subunit GlcD—Brucella suis bv. 2) Accession: AIB28967.1/GI: 648132975; (subunit GlcF—Brucella suis bv. 2) Accession: AIB25578.1/GI: 646251316; (subunit GlcE—Brucella suis bv. 2) Accession: AIB25577.1/GI: 646251315; (subunit GlcD—Brucella suis bv. 2) Accession: AIB25576.1/GI: 646251314; (subunit GlcF—Brucella suis bv. 2) Accession: AIB22223.1/GI: 646247957; (subunit GlcE—Brucella suis bv. 2) Accession: AIB22222.1/GI: 646247956; (subunit GlcD—Brucella suis bv. 2) Accession: AIB22221.1/GI: 646247955; (subunit GlcE—Geomicrobium sp. JCM 19037) Accession: GAK04918.1/GI: 636730043; (subunit GlcF—Geomicrobium sp. JCM 19037) Accession: GAK04917.1/GI: 636730042; (subunit GlcD—Geomicrobium sp. JCM 19037) Accession: GAK04916.1/GI: 636730041; (subunit GlcE—Geomicrobium sp. JCM 19039) Accession: GAK13247.1/GI: 636725850; (subunit GlcE—Geomicrobium sp. JCM 19039) Accession: GAK13246.1/GI: 636725849; (subunit GlcE—Geomicrobium sp. JCM 19039) Accession: GAK13245.1/GI: 636725848; GlcF—Geomicrobium sp. JCM 19039) Accession: GAK13244.1/GI: 636725847; (subunit GlcD—Geomicrobium sp. JCM 19039) Accession: GAK13243.1/GI: 636725846; (subunit GlcD—Bacillus thuringiensis serovar kurstaki str. HD-1) Accession: KEH48238.1/GI: 657515466; (subunit GlcF—Bacillus thuringiensis serovar kurstaki str. HD-1) Accession: KEH48237.1/GI: 657515465; (subunit GlcD—Marinobacter sp. AK21) Accession: KEF30543.1/GI: 656104851; (subunit GlcE—Marinobacter sp. AK21) Accession: KEF30542.1/GI: 656104850; (subunit GlcF—Marinobacter sp. AK21) Accession: KEF30541.1/GI: 656104849; (subunit GlcF—Burkholderia cepacia complex) Accession: WP_021163859.1/GI: 544734260; (subunit GlcF—Sinorhizobium americanum CCGM7) Accession: KEC71436.1/GI: 654362296; (subunit GlcD—Marinobacterium sp. AK27) Accession: KEA65018.1/GI: 653658306; (subunit GlcE—Marinobacterium sp. AK27) Accession: KEA65017.1/GI: 653658305; (subunit GlcF—Marinobacterium sp. AK27) Accession: KEA65016.1/GI: 653658304; (subunit GlcD—Marinobacterium sp. AK27) Accession: KEA61950.1/GI: 653654869; (subunit GlcD—Marinobacterium sp. AK27) Accession: KEA61904.1/GI: 653654823; (subunit GlcF—Brucella suis bv. 2) Accession: AIB32340.1/GI: 646254683; (subunit GlcE—Brucella suis bv. 2) Accession: AIB32339.1/GI: 646254682; (subunit GlcD—Brucella suis bv. 2) Accession: AIB32338.1/GI: 646254681; (subunit GlcF—Brucella suis bv. 2) Accession: AIB18839.1/GI: 646244569; (subunit GlcE—Brucella suis bv. 2) Accession: AIB18838.1/GI: 646244568; subunit GlcD—Brucella suis bv. 2) Accession: AIB18837.1/GI: 646244567; (subunit GlcF—Richelia intracellularis) Accession: CDN13559.1/GI: 605043415; (subunit GlcE—Richelia intracellularis) Accession: CDN13558.1/GI: 605043414; (subunit GlcD—Richelia intracellularis) Accession: CDN17153.1/GI: 605039408; (subunit GlcD—Acidithiobacillus caldus ATCC 51756) Accession: AIA56107.1/GI: 640842222; (subunit GlcE—Acidithiobacillus caldus ATCC 51756) Accession: AIA56106.1/GI: 640842221; (subunit GlcF—Acidithiobacillus caldus ATCC 51756) Accession: AIA56105.1/GI: 640842220; (subunit GlcF—Escherichia coli) Accession: KDN06031.1/GI: 636872977; (subunit GlcE—Escherichia coli) Accession: KDN06030.1/GI: 636872976; (subunit GlcD—Escherichia coli) Accession: KDN06029.1/GI: 636872975; (subunit GlcE—Burkholderia sp. AU4i) Accession: WP_021163858.1/GI: 544734259; (subunit GlcD—Burkholderia sp. AU4i) Accession: WP_021163857.1/GI: 544734258; (subunit GlcE—Burkholderia sp. AU4i) Accession: WP_021163546.1/GI: 544733909; (subunit GlcD—Burkholderia sp. AU4i) Accession: WP_021163545.1/GI: 544733908; (subunit GlcF—Burkholderia sp. AU4i) Accession: WP_021161413.1/GI: 544731662; (subunit GlcD—Lactobacillus plantarum WCFS1) Accession: YP_004888330.1/GI: 380031339; (subunit GlcD—Nitrincola lacisaponensis) Accession: KDE40915.1/GI: 635199988; (subunit GlcE—Nitrincola lacisaponensis) Accession: KDE40914.1/GI: 635199987; (subunit GlcF—Nitrincola lacisaponensis) Accession: KDE40913.1/GI: 635199986; (subunit GlcD—Nitrincola lacisaponensis) Accession: KDE40017.1/GI: 635199084; (subunit GlcD—Lactobacillus casei 12A) Accession: EKP96625.1/GI: 410521667; (subunit GlcD—Burkholderia cenocepacia H111) Accession: CDN64533.1/GI: 590120476; (subunit GlcD—Lactobacillus farraginis DSM 18382=JCM 14108) Accession: GAF36870.1/GI: 588485933; (subunit GlcD—Lactobacillus composti DSM 18527=JCM 14202) Accession: GAF40649.1/GI: 588475006; (subunit GlcD—Lactobacillus composti DSM 18527=JCM 14202) Accession: GAF40969.1/GI: 588474669; (subunit GlcD—Lactobacillus composti DSM 18527=JCM 14202) Accession: GAF41605.1/GI: 588473973; (subunit GlcF—Lactobacillus composti DSM 18527=JCM 14202) Accession: GAF41604.1/GI: 588473972; (subunit GlcD—Burkholderia cenocepacia H111) Accession: CDN59109.1/GI: 590115052; (subunit GlcE—Burkholderia cenocepacia H111) Accession: CDN59108.1/GI: 590115051; (subunit GlcF—Burkholderia cenocepacia H111) Accession: CDN59107.1/GI: 590115050; (subunit GlcD—Burkholderia caribensis MBA4) Accession: EZP25123.1/GI: 612063862; (subunit GlcF—Burkholderia caribensis MBA4) Accession: ETY84259.1/GI: 575865073; (subunit GlcE—Burkholderia caribensis MBA4) Accession: ETY84258.1/GI: 575865072; (subunit GlcE—Burkholderia caribensis MBA4) Accession: ETY80272.1/GI: 575861062; (subunit GlcF—Burkholderia caribensis MBA4) Accession: ETY80271.1/GI: 575861061; (subunit GlcD—Burkholderia caribensis MBA4) Accession: ETY79681.1/GI: 575860465; (subunit GlcE—Burkholderia caribensis MBA4) Accession: ETY79680.1/GI: 575860464; (subunit GlcD—Nodularia spumigena CCY9414) Accession: AHJ31191.1/GI: 585124249; (subunit GlcE—Nodularia spumigena CCY9414) Accession: AHJ28233.1/GI: 585121291; (subunit GlcF—Nodularia spumigena CCY9414) Accession: AHJ28231.1/GI: 585121289; (subunit GlcF—Pseudomonas aeruginosa PA103) Accession: EYU08360.1/GI: 602747861; (subunit GlcD—Pseudomonas aeruginosa PA99) Accession: EYU03926.1/GI: 602743266; (subunit GlcE—Pseudomonas aeruginosa PA99) Accession: EYU03925.1/GI: 602743265; (subunit GlcF—Pseudomonas aeruginosa PA99) Accession: EYU03888.1/GI: 602743226; (subunit GlcE—Pseudomonas aeruginosa PA103) Accession: EYU00813.1/GI: 602740013; (subunit GlcD—Pseudomonas aeruginosa PA103) Accession: EYU00812.1/GI: 602740012; (subunit GlcD—Rubellimicrobium mesophilum DSM 19309) Accession: EYD77315.1/GI: 598664788; (subunit GlcE—Rubellimicrobium mesophilum DSM 19309) Accession: EYD77314.1/GI: 598664787; (subunit GlcF—Rubellimicrobium mesophilum DSM 19309) Accession: EYD77313.1/GI: 598664786; (subunit GlcD—Loktanella hongkongensis DSM 17492) Accession: EYD70622.1/GI: 598657251; (subunit GlcE—Loktanella hongkongensis DSM 17492) Accession: EYD70621.1/GI: 598657250; (subunit GlcF—Loktanella hongkongensis DSM 17492) Accession: EYD70620.1/GI: 598657249; (subunit GlcD—Bacillus anthracis CZC5) Accession: GAE96759.1/GI: 576742197; (subunit GlcF—Bacillus anthracis CZC5) Accession: GAE96758.1/GI: 576742196; (subunit GlcD—Escherichia coli O145:H28 str. RM13514) Accession: AHG10530.1/GI: 573970265; (subunit GlcE—Escherichia coli O145:H28 str. RM13514) Accession: AHG10529.1/GI: 573970264; (subunit GlcF—Escherichia coli O145:H28 str. RM13514) Accession: AHG10528.1/GI: 573970263; (subunit GlcD—Escherichia coli O145:H28 str. RM13516) Accession: AHG16372.1/GI: 573936567; (subunit GlcE—Escherichia coli O145:H28 str. RM13516) Accession: AHG16371.1/GI: 573936566; (subunit GlcF—Escherichia coli O145:H28 str. RM13516) Accession: AHG16370.1/GI: 573936565; (subunit GlcD—Lactobacillus rhamnosus LOCK908) Accession: AGP75006.1/GI: 521380148; (subunit GlcD—Alkalibacterium sp. AK22) Accession: EXJ24332.1/GI: 589844094; (subunit GlcF—Thiorhodococcus sp. AK35) Accession: EXJ15514.1/GI: 589833154; (subunit GlcE—Thiorhodococcus sp. AK35) Accession: EXJ15513.1/GI: 589833153; (subunit GlcD—Thiorhodococcus sp. AK35) Accession: EXJ14044.1/GI: 589831660; (subunit GlcD—Pseudomonas aeruginosa SCV20265) Accession: AHC80185.1/GI: 567369240; (subunit GlcE—Pseudomonas aeruginosa SCV20265) Accession: AHC80184.1/GI: 567369239; (subunit GlcF—Pseudomonas aeruginosa SCV20265) Accession: AHC80183.1/GI: 567369238; (subunit GlcD—Clostridium tyrobutyricum DIVETGP) Accession: CDL90464.1/GI: 587651050; (subunit GlcD—Clostridium tyrobutyricum DIVETGP) Accession: CDL92712.1/GI: 587648629; (subunit GlcE—Escherichia albertii KF1) Accession: AHE60838.1/GI: 569538531; (subunit GlcD—Escherichia coli NA114) Accession: AEG37894.1/GI: 333971089; (subunit GlcF—Achromobacter xylosoxidans NBRC 15126=ATCC 27061) Accession: AHC46259.1/GI: 566050586; (subunit GlcE—Achromobacter xylosoxidans NBRC 15126=ATCC 27061) Accession: AHC46258.1/GI: 566050585; (subunit GlcD—Achromobacter xylosoxidans NBRC 15126=ATCC 27061) Accession: AHC46257.1/GI: 566050584; (Bacillus thuringiensis YBT-1518) Accession: AHA70700.1/GI: 558578565; (subunit GlcF—Bacillus thuringiensis YBT-1518) Accession: AHA70699.1/GI: 558578564; (subunit GlcD—Actinokineospora sp. EG49) Accession: EWC64120.1/GI: 583004694; (subunit GlcD—Actinokineospora sp. EG49); Accession: EWC63240.1/GI: 583003784; (subunit GlcD—Actinokineospora sp. EG49) Accession: EWC61535.1/GI: 583002023; (subunit GlcD—Actinokineospora sp. EG49) Accession: EWC58823.1/GI: 582998387; (subunit GlcD—Actinokineospora sp. EG49) Accession: EWC58610.1/GI: 582998155; (subunit GlcD—Lactobacillus plantarum 16) Accession: AG006960.1/GI: 513034564; (Bacillus thuringiensis serovar thuringiensis str. IS5056) Accession: AGF99954.1/GI: 452103015; (subunit GlcF—Bacillus thuringiensis serovar thuringiensis str. 1S5056) Accession: AGF99953.1/GI: 452103014; (subunit GlcD—Lactobacillus plantarum ZJ316) Accession: AGE38026.1/GI: 448273507; (subunit GlcD—Burkholderia cepacia GG4) Accession: AFQ51978.1/GI: 402251525; (subunit GlcD—Burkholderia sp. KJ006) Accession: AFJ85008.1/GI: 387576292; (subunit GlcE—Burkholderia sp. KJ006) Accession: AFJ85007.1/GI: 387576291; (subunit GlcD—Collitnonas fungivorans Ter331) Accession: AEK60575.1/GI: 340551200; (subunit GlcE—Collimonas fungivorans Ter331) Accession: AEK60574.1/GI: 340551199; (subunit GlcD—Rhodobacter capsulatus SB 1003) Accession: ADE86600.1/GI: 294477212; (subunit GlcE—Rhodobacter capsulatus SB 1003) Accession: ADE86599.1/GI: 294477211; (subunit GlcF—Rhodobacter capsulatus SB 1003) Accession: ADE86598.1/GI: 294477210; (subunit GlcF—Methylacidiphilum infernorum V4) Accession: ACD83652.1/GI: 189186467; (subunit GlcD—Methylacidiphilum infernorum V4) Accession: ACD83650.1/GI: 189186465; (subunit GlcF—Methylacidiphilum infernorum V4) Accession: ACD82952.1/GI: 189185767; (subunit GlcE—Methylacidiphilum infernorum V4) Accession: ACD82951.1/GI: 189185766; (subunit GlcD—Lactobacillus rhamnosus LOCK900) Accession: AGP72079.1/GI: 521376527; (subunit GlcD—Lactobacillus' casei LOCK919) Accession: AGP69226.1/GI: 521372145; (subunit GlcF—Thioalkalivibrio nitratireducens DSM 14787) Accession: AGA33895.1/GI: 430011143; (subunit GlcE—Thioalkalivibrio nitratireducens DSM 14787) Accession: AGA33894.1/GI: 430011142; (subunit GlcD—Thioalkalivibrio nitratireducens DSM 14787) Accession: AGA33893.1/GI: 430011141; (subunit GlcD—Acidithiobacillus caldus SM-1) Accession: AEK59062.1/GI: 340557308; (subunit GlcE—Acidithiobacillus caldus SM-1) Accession: AEK59061.1/GI: 340557307; (subunit GlcF—Acidithiobacillus caldus SM-1) Accession: AEK59060.1/GI: 340557306; (subunit GlcF—Candidatus Pelagibacter sp. IMCC9063) Accession: AEA80927.1/GI: 327486522; (subunit GlcE—Candidatus Pelagibacter sp. IMCC9063) Accession: AEA80926.1/GI: 327486521; (subunit GlcD—Candidatus Pelagibacter sp. IMCC9063) Accession: AEA80925.1/GI: 327486520; (subunit GlcF—Escherichia coli ISC41) Accession: CDL45957.1/GI: 576029023; (subunit GlcD—Escherichia coli ISC41) Accession: CDL48739.1/GI: 576026958; (subunit GlcD—Escherichia coli IS29) Accession: CDK89963.1/GI: −572154655; (subunit GlcE—Escherichia coli IS29) Accession: CDK89962.1/GI: 572154654; (subunit GlcF—Escherichia coli IS29) Accession: CDK89961.1/GI: 572154653; (subunit GlcD—Escherichia coli IS35) Accession: CDL03303.1/GI: 571239987; (subunit GlcE—Escherichia coli IS35) Accession: CDL03302.1/GI: 571239986; (subunit GlcF—Escherichia coli IS35) Accession: CDL03301.1/GI: 571239985; (subunit GlcD—Escherichia coli IS9) Accession: CDK59008.1/GI: 571218906; (subunit GlcF—Escherichia coli IS9) Accession: CDK59453.1/GI: 571218606; (subunit GlcE—Escherichia coli IS9) Accession: CDK59452.1/GI: 571218605; (subunit GlcF—Klebsiella pneumoniae IS22) Accession: CDK76008.1/GI: 571209818; (subunit GlcF—Klebsiella pneumoniae IS22) Accession: CDK76007.1/GI: 571209817; (subunit GlcE—Klebsiella pneumoniae IS22) Accession: CDK76006.1/GI: 571209816; (subunit GlcD—Klebsiella pneumoniae IS22) Accession: CDK76005.1/GI: 571209815; (subunit GlcD—Escherichia coli ISC7) Accession: CDL25131.1/GI: 571185631; (subunit GlcE—Escherichia coli ISC7) Accession: CDL27302.1/GI: 571183498; (subunit GlcF—Escherichia coli ISC7) Accession: CDL27301.1/GI: 571183497; (subunit GlcF—Escherichia coli ISC7) Accession: CDL27300.1/GI: 571183496; (subunit GlcF—Escherichia coli ISC7) Accession: CDL27299.1/GI: 571183495; (subunit GlcD—Escherichia coli IS25) Accession: CDK81232.1/GI: 571178845; (subunit GlcE—Escherichia coli IS25) Accession: CDK81231.1/GI: 571178844; (subunit GlcF—Escherichia coli IS25) Accession: CDK81230.1/GI: 571178843; (subunit GlcD—Bacillus toyonensis BCT-7112) Accession: AHA09808.1/GI: 557475986; (subunit GlcF Bacillus toyonensis BCT-7112) Accession: AHA09807.1/GI: 557475985; (subunit GlcF—Escherichia coli IS5) Accession: CDK49908.1/GI: 568396699; (subunit GlcE—Escherichia coli IS5) Accession: CDK49907.1/GI: 568396698; (subunit GlcD—Escherichia coli IS5) Accession: CDK51787.1/GI: 568394700; (subunit GlcF—Escherichia coli IS1) Accession: CDK49045.1/GI: 568385473; (subunit GlcE—Escherichia coli IS1) Accession: CDK49044.1/GI: 568385472; (subunit GlcD—Escherichia coli IS1) Accession: CDK49093.1/GI: 568385414; (subunit GlcD—Pseudomonas aeruginosa SCV20265) Accession: YP_008985079.1/GI: 568312810; (subunit GlcE—Pseudomonas aeruginosa SCV20265) Accession: YP_008985078.1/GI: 568312809; (subunit GlcF—Pseudomonas aeruginosa SCV20265) Accession: YP_008985077.1/GI: 568312808; (subunit GlcF—Achromobacter xylosoxidans) Accession: WP_024068248.1/GI: 568193884; (subunit GlcE—Achromobacter xylosoxidans) Accession: WP_024068247.1/GI: 568193883; (subunit GlcD—Achromobacter xylosoxidans) Accession: WP_024068246.1/GI: 568193882; (subunit GlcF—Achromobacter xylosoxidans NBRC 15126=ATCC 27061) Accession: YP_008923560.1/GI: 568126913; (subunit GlcE—Achromobacter xylosoxidans NBRC 15126=ATCC 27061) Accession: YP_008923559.1/GI: 568126912; (subunit GlcD—Achromobacter xylosoxidans NBRC 15126=ATCC 27061) Accession: YP_008923558.1/GI: 568126911; (subunit GlcF—Escherichia coli AA86) Accession: EGH37547.1/GI: 330909033; (subunit GlcF—Paenibacillus sp. JCM 10914) Accession: WP_023965351.1/GI: 565884101; (subunit GlcF—Paenibacillus sp. JCM 10914) Accession: WP_023965347.1/GI: 565884097; (subunit GlcD—Paenibacillus sp. JCM 10914) Accession: WP_023965344.1/GI: 565884094; (subunit GlcD—Paenibacillus sp. JCM 10914) Accession: WP_023965343.1/GI: 565884093; (subunit GlcF—Escherichia coli E1777) Accession: ESV01283.1/GI: 559784071; (subunit GlcE—Escherichia coli E1777) Accession: ESV01282.1/GI: 559784070; (subunit GlcD—Escherichia coli E1777) Accession: ESV01281.1/GI: 559784069; (subunit glcF—Loktanella cinnabarina LL-001) Accession: GAD55046.1/GI: 543422469; (subunit gleE—Loktanella cinnabarina LL-001) Accession: GAD55045.1/GI: 543422468; (subunit glcD—Loktanella cinnabarina LL-001) Accession: GAD55044.1/GI: 543422467; (Bacillus thuringiensis YBT-1518) Accession: YP_008817887.1/GI: 558679758; (subunit GlcF—Bacillus thuringiensis YBT-1518) Accession: YP_008817886.1/GI: 558679757; (subunit GlcD—Burkholderia cenocepacia) Accession: WP_023477001.1/GI: 558016316; (subunit GlcE—Burkholderia cenocepacia) Accession: WP_023477000.1/GI: 558016313; (subunit GlcD—Escherichia coli CE516) Accession: ESS91591.1/GI: 557927411; (subunit GlcE—Escherichia coli CE516) Accession: ESS91590.1/GI: 557927410; (subunit GlcF—Escherichia coli CE516) Accession: ESS91589.1/GI: 557927409; (subunit GlcF—Escherichia coli CE549) Accession: ESS91299.1/GI: 557927049; (subunit GlcE—Escherichia coli CE549) Accession: ESS91298.1/GI: 557927048; (subunit GlcD—Escherichia coli CE549) Accession: ESS91297.1/GI: 557927047; (subunit GlcD—Burkholderia cenocepacia KC-01) Accession: ESS38209.1/GI: 557794185; (subunit GlcE—Burkholderia cenocepacia KC-01) Accession: ESS38208.1/GI: 557794184; (subunit GlcF—Lutibaculum baratangense) Accession: WP_023431022.1/GI: 557676479; (subunit GlcE—Lutibaculum baratangense) Accession: WP_023431021.1/GI: 557676475; (subunit GlcD—Lutibaculum baratangense) Accession: WP_023431020.1/GI: 557676472; (subunit GlcD—Bacillus toyonensis BCT-7112) Accession: YP_008784498.1/GI: 557623366; (subunit GlcF—Bacillus toyonensis BCT-7112) Accession: YP_008784497.1/GI: 557623365; (subunit GlcF—Lutibaculum baratangense AMV1) Accession: ESR26376.1/GI: 557406299; (subunit GlcE—Lutibaculum baratangense AMV1) Accession: ESR26375.1/GI: 557406298; (subunit GlcD—Lutibaculum baratangense AMV1) Accession: ESR26374.1/GI: 557406297; (subunit GlcF—Escherichia coli SCD1) Accession: ESA31008.1/GI: 553024184; (subunit GlcE—Escherichia coli SCD1) Accession: ESA31007.1/GI: 553024183; (subunit GlcD—Escherichia coli SCD1) Accession: ESA31006.1/GI: 553024182; (subunit GlcF—Escherichia coli SCD2) Accession: ESA29685.1/GI: 553022841; (subunit GlcE—Escherichia coli SCD2) Accession: ESA29684.1/GI: 553022840; (subunit GlcD—Escherichia coli SCD2) Accession: ESA29683.1/GI: 553022839; (subunit GlcF—Crocosphaera watsonii WH 0401) Accession: CCQ63359.1/GI: 543428392; (subunit GlcE—Crocosphaera watsonii WH 0401) Accession: CCQ63358.1/GI: 543428391; (subunit GlcD—Crocosphaera watsonii WH 0401) Accession: CCQ64344.1/GI: 543427231; (subunit GlcD—Bacillus sp. GeD10) Accession: CCW04120.1/GI: 482659080; (subunit GlcF—Bacillus sp. GeD10) Accession: CCW04119.1/GI: 482659079; (subunit GlcE—Crocosphaera watsonii) Accession: WP_021836465.1/GI: 546233603; (subunit GlcE—Crocosphaera watsonii) Accession: WP_021832550.1/GI: 546225655; (subunit GlcE—Crocosphaera watsonii) Accession: WP_021830164.1/GI: 546209745; (subunit GlcF—Crocosphaera watsonii) Accession: WP_021830163.1/GI: 546209744; (subunit GlcD—Crocosphaera watsonii) Accession: WP_021829695.1/GI: 546207139; (subunit glcF—Loktanella cinnabarina) Accession: WP_021693154.1/GI: 545456215; (subunit gleE—Loktanella cinnabarina) Accession: WP_021693153.1/GI: 545456214; (subunit glcD—Loktanella cinnabarina) Accession: WP_021693152.1/GI: 545456213; (subunit GlcD—Thalassobacter arenae) Accession: WP_021101159.1/GI: 544667804; (subunit GlcE—Thalassobacter arenae) Accession: WP_021101158.1/GI: 544667803; (subunit GlcF—Thalassobacter arenae) Accession: WP_021101157.1/GI: 544667802; (Myxococcus sp.) Accession: ERK85659.1/GI: 544268043; (subunit GlcD—Crocosphaera watsonii WH 0402) Accession: CCQ68714.1/GI: 543534437; (subunit GlcF—Crocosphaera watsonii WH 0402) Accession: CCQ69206.1/GI: 543533753; (subunit GlcE—Crocosphaera watsonii WH 0402) Accession: CCQ69205.1/GI: 543533752; (subunit GlcF—Crocosphaera watsonii WH 0005) Accession: CCQ54800.1/GI: 543527779; (subunit GlcE—Crocosphaera watsonii WH 0005) Accession: CCQ54799.1/GI: 543527778; (subunit GlcD—Crocosphaera watsonii WH 0005) Accession: CCQ57454.1/GI: 543523890; (subunit GlcD—Crocosphaera watsonii WH 8502) Accession: CCQ49822.1/GI: 543517710; (subunit GlcE—Crocosphaera watsonii WH 8502) Accession: CCQ50520.1/GI: 543516532; (subunit GlcF—Crocosphaera watsonii WH 8502) Accession: CCQ50519.1/GI: 543516531; (subunit GlcF—Burkholderia sp. AU4i) Accession: ERJ36103.1/GI: 543288458; (subunit GlcE—Burkholderia sp. AU4i) Accession: ERJ33770.1/GI: 543285528; (subunit GlcD—Burkholderia sp. AU4i) Accession: ERJ33769.1/GI: 543285527; (subunit GlcF—Burkholderia sp. AU4i) Accession: ERJ33412.1/GI: 543284995; (subunit GlcE—Burkholderia sp. AU4i) Accession: ERJ33411.1/GI: 543284994; (subunit GlcD—Burkholderia sp. AU4i) Accession: ERJ33410.1/GI: 543284993; (subunit GlcF—Bacillus) Accession: WP_006917711.1/GI: 493974627; (subunit GlcD—Lactobacillus casei group) Accession: WP_003607194.1/GI: 489703057; (subunit GlcD—Lactobacillus casei group) Accession: WP_003603233.1/GI: 489699094; (subunit GlcD family (Lactobacillus casei group) Accession: WP_003580769.1/GI: 489676531; (subunit GlcD—Acidithiobacillus caldus SM-1) Accession: YP_004749764.1/GI: 340783157; (subunit GlcE—Acidithiobacillus caldus SM-1) Accession: YP_004749763.1/GI: 340783156; (subunit GlcF—Acidithiobacillus caldus SM-1) Accession: YP_004749762.1/GI: 340783155; (subunit GlcF—Nitrosospira sp. APG3) Accession: CCU61652.1/GI: 475054192; (subunit GlcE—Nitrosospira sp. APG3) Accession: CCU61651.1/GI: 475054191; (subunit GlcD—Nitrosospira sp. APG3) Accession: CCU61650.1/GI: 475054190; (subunit GlcF—Nitrosospira sp. APG3) Accession: CCU62734.1/GI: 475052935; (subunit GlcF—Salipiger mucosus DSM 16094) Accession: EPX83070.1/GI: 528101421; (subunit GlcD—Thalassobacter arenae DSM 19593) Accession: EPX81660.1/GI: 528099957; (subunit GlcE—Thalassobacter arenae DSM 19593) Accession: EPX81659.1/GI: 528099956; (subunit GlcF—Thalassobacter arenae DSM 19593) Accession: EPX81658.1/GI: 528099955; (subunit GlcD—Burkholderia cepacia GG4) Accession: YP_006619672.1/GI: 402570328; (subunit GlcD—Lactobacillus plantarum 16) Accession: YP_008120101.1; GI: 513840129; (subunit GlcD—Lactobacillus rhamnosus LOCK908) Accession: YP_008206863.1/GI: 523520783; (subunit GlcD—Lactobacillus rhamnosus LOCK900) Accession: YP_008203896.1/GI: 523517814; (subunit GlcD—Lactobacillus casei LOCK919) Accession: YP_008201043.1/GI: 523514959; (subunit GlcF—Candidatus Pelagibacter sp. 1MCC9063) Accession: YP_004357666.1/GI: 330813427; (subunit GlcE—Candidatus Pelagibacter sp. IMCC9063) Accession: YP_004357665.1/GI: 330813426; (subunit GlcD—Candidatus Pelagibacter sp. IMCC9063) Accession: YP_004357664.1/GI: 330813425; (subunit GlcD—Rhodococcus sp. EsD8) Accession: CCW15042.1/GI: 482629058; (subunit GlcD—Halanaerobium saccharolyticum subsp. saccharolyticum DSM 6643) Accession: CCU78735.1/GI: 460789796; (subunit GlcD—Halanaerobium saccharolyticum subsp. saccharolyticum DSM 6643) Accession: CCU78906.1/GI: 460788890; (subunit GlcD—Escherichia coli E2265) Accession: EPH48303.1/GI: 514335898; (subunit GlcE—Escherichia coli E2265) Accession: EPH48302.1/GI: 514335897; (subunit GlcF—Escherichia coli E2265) Accession: EPH48301.1/GI: 514335896; (subunit GlcD—Lactobacillus plantarum) Accession: WP_016526882.1/GI: 513881376; (subunit GlcD—Lactobacillus plantarum) Accession: WP_016511414.1/GI: 513804516; (subunit GlcD—Lactobacillus plantarum IPLA88) Accession: EPD24615.1/GI: 511781180; (subunit GlcD—Lactobacillus paracasei) Accession: WP_016388090.1/GI: 511754494; (subunit GlcD—Lactobacillus paracasei) Accession: WP_016385692.1/GI: 511751024; (subunit GlcD—Lactobacillus paracasei) Accession: WP_016384795.1/GI: 511749995; (subunit GlcD—Burkholderia sp. KJ006) Accession: YP_006331739.1/GI: 387901400; (subunit GlcE—Burkholderia sp. KJ006) Accession: YP_006331738.1/GI: 387901399; (subunit GlcD—Lactobacillus paracasei subsp. paracasei Lpp225) Accession: EPC38404.1/GI: 511400394; (subunit GlcD—Lactobacillus paracasei subsp. paracasei Lpp120) Accession: EPC34604.1/GI: 511396438; (subunit GlcD—Lactobacillus paracasei subsp. paracasei Lpp22) Accession: EPC33420.1/GI: 511395164; (subunit GlcD—Lactobacillus paracasei subsp. paracasei Lpp223) Accession: EPC32806.1/GI: 511394511; (subunit GlcD—Lactobacillus paracasei subsp. paracasei Lpp46) Accession: EPC26602.1/GI: 511387879; (subunit GlcD—Lactobacillus paracasei subsp. paracasei Lpp17) Accession: EPC22692.1/GI: 511383770; (subunit GlcD—Lactobacillus paracasei subsp. paracasei Lpp226) Accession: EPC21198.1/GI: 511382182; (subunit GlcD—Lactobacillus paracasei subsp. paracasei Lpp122) Accession: EPC19465.1/GI: 511380408; (subunit GlcD—Lactobacillus paracasei subsp. tolerans Lp17) Accession: EPC15010.1/GI: 511375735; (subunit GlcD—Lactobacillus paracasei subsp. paracasei Lpp230) Accession: EPC12340.1/GI: 511372931; (Bacillus thuringiensis serovar thuringiensis str. IS5056) Accession: YP_007477703.1/GI: 452197622; (subunit GlcF—Bacillus thuringiensis serovar thuringiensis str. IS5056) Accession: YP_007477702.1/GI: 452197621; (subunit GlcD—Lactobacillus plantarum ZJ316) Accession: YP_007413172.1/GI: 448820010; (subunit GlcF—Thioalkalivibrio nitratireducens DSM 14787) Accession: YP_007217376.1/GI: 430761519; (subunit GlcE—Thioalkalivibrio nitratireducens DSM 14787) Accession: YP_007217375.1/GI: 430761518; (subunit GlcD—Thioalkalivibrio nitratireducens DSM 14787) Accession: YP_007217374.1/GI: 430761517; (subunit GlcD—Escherichia coli NA114) Accession: YP_006140168.1/GI: 386620588; (subunit GlcD—Rhodobacter capsulatus SB 1003) Accession: YP_003579007.1/GI: 294678392; (subunit GlcE—Rhodobacter capsulatus SB 1003) Accession: YP_003579006.1/GI: 294678391; (subunit GlcF—Rhodobacter capsulatus SB 1003) Accession: YP_003579005.1/GI: 294678390; (subunit GlcF—Methylacidiphilum infernorum V4) Accession: YP_001940250.1/GI: 189219609; (subunit GlcD—Methylacidiphilum infernorum V4) Accession: YP_001940248.1/GI: 189219607; (subunit GlcF—Methylacidiphilum infernorum V4) Accession: YP_001939550.1/GI: 189218909; (subunit GlcE—Methylacidiphilum infernorum V4) Accession: YP_001939549.1/GI: 189218908; (subunit GlcD—Rhodobacter sp. AKP1) Accession: WP_009563066.1/GI: 497248849; (subunit GlcE—Rhodobacter sp. AKP1) Accession: WP_009563065.1/GI: 497248848; (subunit GlcF—Rhodobacter sp. AKP1) Accession: WP_009563064.1/GI: 497248847; (subunit GlcF—Caenispirillum salinarum) Accession: WP_009542738.1/GI: 497228476; (subunit GlcE—Caenispirillum salinarum) Accession: WP_009542737.1/GI: 497228475; (subunit GlcD—Caenispirillum salinarum) Accession: WP_009542736.1/GI: 497228474; (subunit GlcE—Crocosphaera watsonii) Accession: WP_007309487.1/GI: 494520033; (subunit GlcE—Herbaspirillum frisingense) Accession: WP_006464790.1/GI: 493510439; (subunit GlcD—Clostridiaceae bacterium L21-TH-D2) Accession: WP_006311157.1/GI: 493354535; (subunit GlcD—Lactobacillus rhamnosus) Accession: WP_005716550.1/GI: 492011914; (subunit GlcD—Halanaerobium saccharolyticum) Accession: WP_005488303.1/GI: 491630764; (subunit GlcD—Halanaerobium saccharolyticum) Accession: WP_005488139.1/GI: 491630600; (subunit GlcD—Amycolatopsis azurea) Accession: WP_005160695.1/GI: 491302692; (subunit GlcD—Amycolatopsis azurea) Accession: WP_005150580.1/GI: 491292564; (subunit GlcD—Lactobacillus plantarum) Accession: WP_015379716.1/GI: 505192614; (subunit GlcF—Thioalkalivibrio nitratireducens) Accession: WP_015259016.1/GI: 505071914; (subunit GlcE—Thioalkalivibrio nitratireducens) Accession: WP_015259015.1/GI: 505071913; (subunit GlcD—Thioalkalivibrio nitratireducens) Accession: WP_015259014.1/GI: 505071912; (subunit GlcD (Lactobacillus casei) Accession: WP_004469756.1/GI: 490604736; (subunit GlcF—Bacillus atrophaeus) Accession: WP_010789427.1/GI: 498487739; (subunit GlcD—Bacillus atrophaeus) Accession: WP_010789426.1/GI: 498487738; (subunit GlcF—Nitrosospira sp. APG3) Accession: WP_004178149.1/GI: 490282255; (subunit GlcF—Nitrosospira sp. APG3) Accession: WP_004175407.1/GI: 490279470; (subunit GlcE—Nitrosospira sp. APG3) Accession: WP_004175406.1/GI: 490279469; (subunit GlcD—Nitrosospira sp. APG3) Accession: WP_004175404.1/GI: 490279467; (subunit GlcD—Lactobacillus plantarum) Accession: WP_003646405.1/GI: 489742349; (subunit GlcD—Lactobacillus casei) Accession: WP_003605020.1/GI: 489700883; (subunit GlcD—Lactobacillus casei) Accession: WP_003591621.1/GI: 489687412; (subunit GlcD—Lactobacillus casei) Accession: WP_003576201.1/GI: 489671946; (subunit GlcD—Pseudomonas aeruginosa) Accession: WP_003162880.1/GI: 489254909; (subunit GlcD—Cystobacter fuscus) Accession: WP_002627940.1/GI: 488703965; (subunit GlcF—Grimontia sp. AK16) Accession: WP_002536222.1/GI: 488492778; (subunit GlcE—Grimontia sp. AK16) Accession: WP_002536220.1/GI: 488492776; (subunit GlcD—Grimontia sp. AK16) Accession: WP_002536218.1/GI: 488492774; (subunit GlcF—Grimontia sp. AK16) Accession: EOD81473.1/GI: 486374710; (subunit GlcE—Grimontia sp. AK16) Accession: EOD81472.1/GI: 486374709; (subunit GlcD—Grimontia sp. AK16) Accession: EOD81471.1/GI: 486374708; (subunit GlcF—Bacillus atrophaeus UCMB-5137) Accession: EOB36005.1/GI: 485131200; (subunit GlcD—Bacillus atrophaeus UCMB-5137) Accession: EOB36004.1/GI: 485131199; (subunit GlcD—Clostridiaceae bacterium L21-TH-D2) Accession: EOD00877.1/GI: 485596261; (subunit GlcE—Herbaspirillum frisingense GSF30) Accession: EOA03090.1/GI: 481868141; (subunit GlcD—Pseudomonas aeruginosa 18A) Accession: CCQ87198.1/GI: 451755860; (subunit GlcD—Lactobacillus plantarum UCMA 3037) Accession: EMP43043.1/GI: 468443300; (subunit GlcF—Pseudomonas savastanoi pv. savastanoi NCPPB 3335) Accession: EFH98862.1/GI: 298157783; (subunit GlcE—Pseudomonas savastanoi pv. savastanoi NCPPB 3335) Accession: EFH98861.1/GI: 298157782; (subunit GlcD—Pseudomonas savastanoi pv. savastanoi NCPPB 3335) Accession: EFH98860.1/GI: 298157781; (subunit GlcD—Amycolatopsis azurea DSM 43854) Accession: EMD29767.1/GI: 449424468; (subunit GlcD—Amycolatopsis azurea DSM 43854) Accession: EMD25329.1/GI: 449419808; (subunit GlcD—Rhodobacter sp. AKP1) Accession: EKX58087.1/GI: 428189534; (subunit GlcE—Rhodobacter sp. AKP1) Accession: EKX58086.1/GI: 428189533; (subunit GlcF—Rhodobacter sp. AKP1) Accession: EKX58085.1/GI: 428189532; (subunit GlcF—Caenispirillum salinarum AK4) Accession: EKV26455.1/GI: 425877724; (subunit GlcE—Caenispirillum salinarum AK4) Accession: EKV26454.1/GI: 425877723; (subunit GlcD—Caenispirillum salinarum AK4) Accession: EKV26453.1/GI: 425877722; (subunit GlcD—Lactobacillus rhamnosus LRHMDP2) Accession: EKS50474.1/GI: 411183335; (subunit GlcD—Lactobacillus rhamnosus LRHMDP3) Accession: EKS48724.1/GI: 411181555; (subunit GlcD—Lactobacillus casei Lpc-37) Accession: EKQ29053.1/GI: 410555090; (subunit GlcD—Lactobacillus casei Lc-10) Accession: EKQ27271.1/GI: 410553268; (subunit GlcD—Lactobacillus casei UW4) Accession: EKQ19867.1/GI: 410545576; (subunit GlcD—Lactobacillus casei UW1) Accession: EKQ19363.1/GI: 410545055; (subunit GlcD family (Lactobacillus casei UCD174) Accession: EKQ17333.1/GI: 410542932; (subunit GlcD—Lactobacillus casei T71499) Accession: EKQ12735.1/GI: 410538178; (subunit GlcD—Lactobacillus casei A2-362) Accession: EKQ08992.1/GI: 410534338; (subunit GlcD—Lactobacillus casei M36) Accession: EKQ08475.1/GI: 410533809; (subunit GlcD—Lactobacillus casei CRF28) Accession: EKQ05619.1/GI: 410530856; (subunit GlcD—Lactobacillus casei 21/1) Accession: EKQ00611.1/GI: 410525713; (subunit GlcD—Lactobacillus casei 32G) Accession: EKP97870.1/GI: 410522932; (subunit GlcD—Rhodovulum sp. PH10) Accession: EJW11438.1/GI: 402499743; (subunit GlcE—Rhodovulum sp. PH10) Accession: EJW11437.1/GI: 402499742; (subunit GlcF—Rhodovulum sp. PH10) Accession: EJW11435.1/GI: 402499740; (subunit GlcD—Lactococcus raffinolactis 4877) Accession: CCK18617.1/GI: 399207544; (subunit GlcD—Lactobacillus pentosus KCA1) Accession: EIW15038.1/GI: 392437148; (subunit GlcE—Methylacidiphilum fumariolicum SolV) Accession: CCG91738.1/GI: 384526958; (subunit GlcF—Methylacidiphilum fumariolicum SolV) Accession: CCG91737.1/GI: 384526957; (subunit GlcF—Methylacidiphilum fumariolicum SolV) Accession: CCG92195.1/GI: 384526449; (subunit GlcD—Methylacidiphilum fumariolicum SolV) Accession: CCG92194.1/GI: 384526448; (subunit GlcD—Lactobacillus plantarum WCFS1) Accession: CCC77816.1/GI: 342240582; (subunit GlcD—Lactobacillus plantarum subsp. plantarum NC8) Accession: EHS83950.1/GI: 376010625; (subunit GlcD—Burkholderia cenocepacia H111) Accession: CCE46351.1/GI: 358076106; (subunit GlcF—Burkholderia cenocepacia H111) Accession: CCE51249.1/GI: 358071291; (subunit GlcE—Burkholderia cenocepacia H111) Accession: CCE51248.1/GI: 358071290; (subunit GlcD—Burkholderia cenocepacia H111) Accession: CCE51247.1/GI: 358071289; (subunit GlcE—Crocosphaera watsonii WH 0003) Accession: EHJ14361.1/GI: 357265620; (subunit GlcF—Crocosphaera watsonii WH 0003) Accession: EHJ14350.1/GI: 357265608; (subunit GlcD—Crocosphaera watsonii WH 0003) Accession: EHJ10223.1/GI: 357260887; (subunit GlcE—endosymbiont of Riftia pachyptila (vent Ph05)) Accession: EGV51965.1/GI: 344225612; (subunit GlcF—endosymbiont of Riftia pachyptila (vent Ph05)) Accession: EGV51964.1/GI: 344225611; (subunit GlcD—Escherichia coli EC4100B) Accession: EFW76461.1/GI: 320201886; (subunit GlcE—Escherichia coli EC4100B) Accession: EFW76460.1/GI: 320201885; (subunit GlcF—Escherichia coli EC4100B) Accession: EFW76459.1/GI: 320201884; (subunit GlcD—Escherichia coli WV 060327) Accession: EFW69751.1/GI: 320195122; (subunit GlcE—Escherichia coli WV 060327) Accession: EFW69750.1/GI: 320195121; (subunit GlcF—Escherichia coli WV 060327) Accession: EFW69749.1/GI: 320195120; (subunit GlcF—Shigella boydii ATCC 9905) Accession: EFW55921.1/GI: 320181000; (subunit GlcE—Shigella boydii ATCC 9905) Accession: EFW55920.1/GI: 320180999; (subunit GlcD—Shigella boydii ATCC 9905) Accession: EFW55919.1/GI: 320180998; (subunit GlcD—Shigella boydii ATCC 9905) Accession: EFW55918.1/GI: 320180997; (subunit GlcD—Pseudomonas fluorescens F113) Accession: AEV61992.1/GI: 359759913; (subunit GlcE—Pseudomonas fluorescens F113) Accession: AEV61991.1/GI: 359759912; (subunit GlcF—Pseudomonas fluorescens F113) Accession: AEV61990.1/GI: 359759911; (subunit GlcE—Escherichia coli A35218R) Accession: ESE33291.1/GI: 553718318; (subunit GlcF—Escherichia coli A35218R) Accession: ESE33290.1/GI: 553718317; (subunit GlcF—Escherichia coli A25922R) Accession: ESE30344.1/GI: 553715255; (subunit GlcE—Escherichia coli A25922R) Accession: ESE30343.1/GI: 553715254; (subunit GlcF—Escherichia coli 908691) Accession: ESE21639.1/GI: 553706155; (subunit GlcE—Escherichia coli 908691) Accession: ESE21638.1/GI: 553706154; (subunit GlcE—Escherichia coli 910096-2) Accession: ESE18833.1/GI: 553703214; (subunit GlcF—Escherichia coli 910096-2) Accession: ESE18831.1/GI: 553703212; (subunit GlcF—Escherichia coli 908675) Accession: ESE17737.1/GI: 553702096; (subunit GlcE—Escherichia coli 908675) Accession: ESE17736.1/GI: 553702095; (subunit GlcF—Escherichia coli 908632) Accession: ESE05561.1/GI: 553689341; (subunit GlcE—Escherichia coli 908632) Accession: ESE05560.1/GI: 553689340; (subunit GlcF—Escherichia coli 908624) Accession: ESD97562.1/GI: 553680913; (subunit GlcE—Escherichia coli 908624) Accession: ESD97561.1/GI: 553680912; (subunit GlcF—Escherichia coli 908616) Accession: ESD86368.1/GI: 553669064; (subunit GlcE—Escherichia coli 908616) Accession: ESD86367.1/GI: 553669063; (subunit GlcF—Escherichia coli 908573) Accession: ESD77743.1/GI: 553659862; (subunit GlcE—Escherichia coli 908573) Accession: ESD77742.1/GI: 553659861; (subunit GlcF—Escherichia coli 908524) Accession: ESD50621.1/GI: 553631397; (subunit GlcE—Escherichia coli 908524) Accession: ESD50620.1/GI: 553631396; (subunit GlcE—Escherichia coli 907889) Accession: ESD41111.1/GI: 553621475; (subunit GlcF—Escherichia coli 907889) Accession: ESD41110.1/GI: 553621474; (subunit GlcF—Escherichia coli 907892) Accession: ESD39087.1/GI: 553619321; (subunit GlcE—Escherichia coli 907892) Accession: ESD39086.1/GI: 553619320; (subunit GlcE—Escherichia coli 908519) Accession: ESD37595.1/GI: 553617775; (subunit GlcF—Escherichia coli 908519) Accession: ESD37594.1/GI: 553617774; (subunit GlcF—Escherichia coli 907391) Accession: ESC99480.1/GI: 553577909; (subunit GlcE—Escherichia coli 907391) Accession: ESC99479.1/GI: 553577908; (subunit GlcE—Escherichia coli 907779) Accession: ESA94412.1/GI: 553367942; (subunit GlcF—Escherichia coli 907779) Accession: ESA94411.1/GI: 553367941; (subunit GlcF—Escherichia coli 113290) Accession: ESA73887.1/GI: 553346580; (subunit GlcE—Escherichia coli 113290) Accession: ESA73885.1/GI: 553346578; (subunit GlcD—Pseudomonas fluorescens F113) Accession: YP_005207387.1/GI: 378949899; (subunit GlcE—Pseudomonas fluorescens F113) Accession: YP_005207386.1/GI: 378949898; (subunit GlcF—Pseudomonas fluorescens F113) Accession: YP_005207385.1/GI: 378949897.

The invention may also use variants of the glycolate dehydrogenase polypeptide(s) herein described. Variants include, but are not limited to, deletions, additions, substitutions, insertions of e.g. 1 to 20 amino acids, such as 1 to 10, or 1 to 5). Generally, amino acid sequence variants of glycolate dehydrogenase polypeptide(s) described herein will have at least 60%, to 70%, e.g., preferably 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, to 79%, generally at least 80%, e.g., 81% to 84%, at least 85%, e.g., 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, to 98% and 99% sequence identity to the amino acid sequences of the glycolate dehydrogenases explicitly described herein, and will retain glycolate dehydrogenase activity. Variant glycolate dehydrogenase may have at least 60% sequence identity to the amino acid sequence of SEQ ID No 6. Variant glycolate dehydrogenase may have at least 60% sequence identity to the amino acid sequence of SEQ ID No 9. Variant glycolate dehydrogenase may have at least 60% sequence identity to the amino acid sequence of SEQ ID No 3, 4 and 5. Variant glycolate dehydrogenase may have at least 95% sequence identity to the amino acid sequence of SEQ ID No 6. Variant glycolate dehydrogenase may have at least 95% sequence identity to the amino acid sequence of SEQ ID No 9. Variant glycolate dehydrogenase may have at least 95% sequence identity to the amino acid sequence of SEQ ID No 3, 4 and 5. Generally, nucleotide sequence variants have at least 60%, to 70%, e.g., preferably 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, to 79%, generally at least 80%, e.g., 81% to 84%, at least 85%, e.g., 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, to 98% and 99% sequence identity to the nucleotide sequences encoding the glycolate dehydrogenases described herein, and the encoded products retain glycolate dehydrogenase activity. Variant nucleic acids encoding glycolate dehydrogenase polypeptide(s) may have at least 60% sequence identity to the nucleotide sequence of SEQ ID No 10 from the nucleotide position 3666-6845. Variant nucleic acids encoding glycolate dehydrogenase polypeptide(s) may have at least 95% sequence identity to the nucleotide sequence of SEQ ID No 10 from the nucleotide position 3666-6845.

The nucleic acid molecules encoding glycolate dehydrogenase polypeptide(s) as described herein may be modified, for example, by codon optimization to facilitate expression in heterologous cells, as described elsewhere for SBPases. The glycolate dehydrogenase polypeptide(s) may be linked to chloroplast targeting signals as described elsewhere. The nucleic acids may be linked to regulatory regions as described above, and the nucleic acids may be either inserted in plastid, such as chloroplast genomes, or in the nuclear genome as described above in the context of expression of SBPase polypeptide(s). Each of the embodiments described for SBPase expression should be considered to have been disclosed mutatis mutandis in connection with the glycolate dehydrogenases and encoding nucleotides herein mentioned.

It is expected that the methods of the invention will be suitable for any plant, including for monocotyledonous, dicotyledonous Angiosperm plants, as well as for gymnosperm plants. The methods of the invention will be particularly suitable for crop plants, including wheat, rice, millet, corn, sorghum, rye, oats, sugarcane, cotton, soybean or Brassica (oilseed) plants including mustard.

Plants according to the invention can be used in a conventional breeding scheme to produce more transformed plants with the same characteristics or to introduce the chimeric gene according to the invention in other varieties of the same or related plant species, or in hybrid plants. Seeds obtained from the transformed plants contain the chimeric genes of the invention as a stable genomic insert and are also encompassed by the invention. The present invention also relates to any products such as meal, oil, fibers etc. which are obtained by processing the plants, parts thereof, or seeds of the invention.

As used herein “comprising” is to be interpreted as specifying the presence of the stated features, integers, steps or components as referred to, but does not preclude the presence or addition of one or more features, integers, steps or components, or groups thereof. Thus, e.g., a nucleic acid or protein comprising a sequence of nucleotides or amino acids, may comprise more nucleotides or amino acids than the actually cited ones, i.e., be embedded in a larger nucleic acid or protein. A chimeric gene comprising a DNA region, which is functionally or structurally defined, may comprise additional DNA regions etc.

The following non-limiting examples describe the generation of transgenic plants expressing simultaneously glycolate dehydrogenase enzymes and SBPase and the effect on growth, biomass and carbon assimilation.

Unless states otherwise in the Examples, all recombinant techniques are carried out according to standard protocols as described in “Sambrook J and Russell D W (eds.) (2001) Molecular Cloning: A Laboratory Manual, 3rd Edition, Cold Spring Harbor Laboratory Press, New York” and in “Ausubel F A, Brent R, Kingston R E, Moore D D, Seidman J G, Smith J A and Struhl K (eds.) (2006) Current Protocols in Molecular Biology. John Wiley & Sons, New York”.

Standard materials and references are described in “Croy R D D (ed.) (1993) Plant Molecular Biology LabFax, BIOS Scientific Publishers Ltd., Oxford and Blackwell Scientific Publications, Oxford” and in “Brown T A, (1998) Molecular Biology LabFax, 2nd Edition, Academic Press, San Diego”. Standard materials and methods for polymerase chain reactions (PCR) can be found in “McPherson M J and Møller S G (2000) PCR (The Basics), BIOS Scientific Publishers Ltd., Oxford” and in “PCR Applications Manual, 3rd Edition (2006), Roche Diagnostics GmbH, Mannheim or www.roche-applied-science.com”.

The sequence listing contained in the file named “BCS14-2011-W01 ST25.txt”, which is 78 kilobytes (size as measured in Microsoft Windows®), contains 12 sequences. SEQ ID NO: 1 through SEQ ID NO: 12 is filed herewith by electronic submission and is incorporated by reference herein.

In the description and examples, reference is made to the following sequences:

SEQ ID 1: nucleotide sequence of the coding sequence of the sedoheptulose 1,7-bisphosphate gene of Oryza sativa
SEQ ID 2: amino acid sequence of the SBPase from Oryza sativa
SEQ ID 3: amino acid sequence of the GlcD subunit from Escherichia coli
SEQ ID 4: amino acid sequence of the GlcE subunit from Escherichia coli
SEQ ID 5: amino acid sequence of the GlcD subunit from Escherichia coli
SEQ ID 6: amino acid sequence of the Chlamydomonas rheinhardtii mature glycolate dehydrogenase
SEQ ID 7: amino acid sequence of the Chlamydomonas rheinhardtii mature glycolate dehydrogenase (truncated)
SEQ ID 8: amino acid sequence of the Synechocystis mature glycolate dehydrogenase
SEQ ID 9: amino acid of glycolate dehydrogenase from Arabidopsis thaliana
SEQ ID 10: nucleotide sequence of the T-DNA of vector PTCD155
SEQ ID 11: nucleotide sequence of the T-DNA of vector PTMV548
SEQ ID 12: nucleotide sequence of the T-DNA of vector PTCD163

EXAMPLES

Example 1. Effect of Combined Expression of SPBase and Glycolate Dehydrogenase in Arabidopsis thaliana

Arabidopsis thaliana plants comprising a recombinant gene encoding glycolate dehydrogenase from Chlamydomonas rheinhardtii, as described in WO2010/012796, were crossed with Arabidopsis thaliana plants comprising a recombinant gene encoding SBPase/FBPase (as described in Miyagawa et al. 2001, supra) and progeny plants comprising both chimeric genes were selected.

Plants comprising both transgenes encoding glycolatedehydrogenase GDH and SBPase were compared to plant that only contain one transgene encoding GDH or only SBPase. In a first experiment, whereby plants were grown under light levels of approximately 120 μE, an increase of 5% in rosette diameter, 10% in fresh weight and 11% in dry weight was observed.

TABLE 1A
	AV		Av Fresh		Av dry
	rosette		weight		weight
Genotype	(cm)	SE	(g)	SE	(g)	SE
GDH/SBPase	12.52	0.2384	1.49	0.0711	0.098	2.4
GDH only or	11.90	0.3894	1.36	0.0941	0.088	3.6
SBPase only
Ttest	0.157		0.244		0.23
% increase	5.19		10.01		11

In a second experiment, whereby plants were grown under light levels of approximately 120 μE, an increase of 4% in rosette diameter, 11% in fresh weight and 7% in dry weight was observed.

TABLE 1B
	AV		Av Fresh		Av dry
	rosette		weight		weight
Genotype	(cm)	SE	(g)	SE	(g)	SE
GDH/SBPase	12.5	0.2	2.0	0.1	0.15	0.0
GDH only or	12.0	0.2	1.7	0.1	0.14	0.0
SBPase only
Ttest	0.1		0.1		0.3
% increase	4.2		11.0		6.9

In a third experiment, plants were grown under higher light levels of approximately 250 μE. No positive impact on growth was observed in this experiment.

Example 2. Effect of Combined Expression of SBPase and Glycolate Dehydrogenase in Wheat

Using conventional recombinant gene techniques, a recombinant gene encoding glycolate dehydrogenase under control of a rice Rubisco small subunit promoter was constructed by operably linking the following DNA regions:

• • a. A DNA region having a sequence including the promoter region of the ribulosebisphosphate carboxylase small subunit gene of Oryza sativa (rice) (Nomura et al., 2000) (SEQ ID No 10 between nucleotides 75-2824)
  • b. A DNA region coding for the first intron of the Actin 1 gene of Oryza sativa (rice) (Mc Elroy et al., 1990). (SEQ ID No 10 between nucleotides 2825-3293)
  • c. A DNA region encoding the optimized transit peptide, containing sequence of the RuBisCO small subunit genes of Zea mays (corn) and Helianthus annuus (sunflower) (Lebrun et al., 1996), adapted to wheat codon usage (SEQ ID No 10 between nucleotides 3294-3665)
  • d. A DNA region encoding the mature part of the glycolate dehydrogenase gene of Chlamydomonas reinhardtii (Nakamura et al., 2005), adapted to wheat codon usage (SEQ ID No 10 between nucleotides 3666-6845)
  • e. A DNA region sequence including the 3′ untranslated region of the nopaline synthase gene from the T-DNA of pTiT37 (Depicker et al., 1982) (SEQ ID No 10 between nucleotides 6846-7143)

Using conventional recombinant gene techniques, a recombinant gene encoding glycolate dehydrogenase under control of a rice fructose 1,6 bisphosphatase gene promoter was constructed by operably linking the following DNA regions:

• • a. A DNA region having a sequence including the promoter region of the fructose 1,6 bisphosphatase gene of Oryza sativa (rice) (Si et al., 2003) (SEQ ID No 11 between nucleotides 75-1272)
  • b. A DNA region coding for the first intron of the Actin 1 gene of Oryza sativa (rice) (Mc Elroy et al., 1990). (SEQ ID No 11 between nucleotides 1273-1741)
  • c. A DNA region encoding the optimized transit peptide, containing sequence of the RuBisCO small subunit genes of Zea mays (corn) and Helianthus annuus (sunflower) (Lebrun et al., 1996), adapted to wheat codon usage (SEQ ID No 11 between nucleotides 1742-2113)
  • d. A DNA region encoding the mature part of the glycolate dehydrogenase gene of Chlamydomonas reinhardtii (Nakamura et al., 2005), adapted to wheat codon usage (SEQ ID No 11 between nucleotides 2114-5293)
  • e. A DNA region sequence including the 3′ untranslated region of the nopaline synthase gene from the T-DNA of pTiT37 (Depicker et al., 1982) (SEQ ID No 11 between nucleotides 5294-5591)

Using conventional recombinant gene techniques, a recombinant gene encoding SBPase under control of a a rice RuBisCO small subunit gene promoter was constructed by operably linking the following DNA regions:

• • a. A DNA region having a the promoter region of the ribulosebisphosphate carboxylase small subunit gene of Oryza sativa (rice) (Nomura et al., 2000) (SEQ ID No 12 between nucleotides 2010-4759 counter clockwise)
  • b. A DNA region coding for the first intron of the actin 1 gene of Oryza sativa (rice) (Mc Elroy et al., 1990). (SEQ ID No 12 between nucleotides 1541-2009 counter clockwise)
  • c. A DNA region encoding coding sequence of the sedoheptulose-1,7-bisphosphatase gene of Oryza sativa (rice) (Feng et al., 2007) (SEQ ID No 12 between nucleotides 362-1540)
  • d. A DNA region sequence including the 3′ untranslated region of the octopine synthase gene of Agrobacterium (De Greve et al 1982) (SEQ ID No 12 between nucleotides 26-361 counter clockwise).

The recombinant gene encoding encoding glycolate dehydrogenase under control of a rice RuBisCO small subunit promoter was combined with the glufosinate tolerance gene (bar) as selectable marker and cloned between the T-DNA borders of pTCD155. The nucleotide sequence of the T-DNA of this vector is included in the sequence listing as SEQ ID No 10. A list of the features of this sequence is included as Table 2.

In a similar manner, the recombinant gene encoding encoding glycolate dehydrogenase under control of a rice fructose 1,6 bisphosphatase gene promoter was combined with the glufosinate tolerance gene (bar) as selectable marker and cloned between the T-DNA borders of pTMV548. The nucleotide sequence of the T-DNA of this vector is included in the sequence listing as SEQ ID No 11. A list of the features of this sequence is included as Table 3.

In addition, the recombinant gene encoding SBPase under control of a rice Rubisco small subunit promoter was combined with recombinant gene encoding glycolate dehydrogenase under control of a rice RuBisCo small subunit promoter and the glufosinate tolerance gene (bar) as selectable marker and cloned between the T-DNA borders of pTCD163. The nucleotide sequence of the T-DNA of this vector is included in the sequence listing as SEQ ID No 12. A list of the features of this sequence is included as Table 4.

The T-DNA vectors were introduced into Agrobacterium tumefaciens comprising a helper Ti-plasmid and the resulting strains were used to generate transgenic wheat plants, according to the method described in WO2011/013764.

Transgenic wheat plants are assayed for biomass production, seed yield and CO₂ assimilation. Wheat plants expressing both SBPasc and glycolate dehydrogenase have an increased biomass (dry weight), increased seed yield and increased CO₂ assimilation when compared to transgenic wheat plant expression only glycolate dehydrogenase.

TABLE 2
Description of the genetic elements of pTCD155.
Nucleotide
positions	Orientation	Description
1-25		RB: right border repeat from the T-DNA of
		Agrobacterium tumefaciens (Zambryski, 1988)
75-2824	Clockwise	PrbcSOs: sequence including the promoter region of the
		ribulosebisphosphate carboxylase small subunit gene of
		Oryza sativa (rice) (Nomura et al., 2000)
2825-3293	Clockwise	intron_act1Os: first intron of the actin 1 gene of Oryza
		sativa (rice) (Mc Elroy et al., 1990).
3294-3665	Clockwise	TPotpC-1Pm: coding sequence of the optimized transit
		peptide, containing sequence of the RuBisCO small
		subunit genes of Zea mays (corn) and
		Helianthus annuus (sunflower) (Lebrun et al.,
		1996), adapted to wheat codon usage
3666-6845	Clockwise	gdhCrh-2Pd: coding sequence of the mature part of the
		glycolate dehydrogenase gene of Chlamydomonas
		reinhardtii (Nakamura et al., 2005), adapted to wheat
		codon usage
6846-7143	Clockwise	3′ nos: sequence including the 3′ untranslated
		region of the nopaline synthase gene from the T-DNA of
		pTiT37 (Depicker et al., 1982)
7220-8056	Clockwise	P35S3: sequence including the promoter region of the
		Cauliflower Mosaic Virus 35S transcript
		(Odell et al., 1985)
8057-8608	Clockwise	bar: coding sequence of the phosphinothricin
		acetyltransferase gene of Streptomyces hygroscopicus
		(Thompson et al., 1987).
8609-8892	Clockwise	3′ nos: sequence including the 3′ untranslated
		region of the nopaline synthase gene from the T-DNA of
		pTiT37 (Depicker et al., 1982)
8989-9013		LB: left border repeat from the T-DNA of Agrobacterium
		tumefaciens (Zambryski, 1988)

TABLE 3
Description of the genetic elements of pTMV548.
Nucleotide
positions	Orientation	Description
1-25		RB: right border repeat from the T-DNA of Agrobacterium
		tumefaciens (Zambryski, 1988
75-1272	Clockwise	Pfbp1Os: sequence including the promoter region of the
		cytosolic fructose-1,6-bisphosphatase gene of Oryza sativa
		(Si et al., 2003)
1273-1741	Clockwise	intron_act1Os: first intron of the actin 1 gene of Oryza
		sativa (rice) (Mc Elroy et al., 1990).
1742-2113	Clockwise	TPotpC-1Pm: coding sequence of the optimized transit
		peptide, containing sequence of the RuBisCO small
		subunit genes of Zea mays (corn) and Helianthus annuus
		(sunflower) (Lebrun et al., 1996), adapted to wheat codon
		usage
2114-5293	Clockwise	gdhCrh-2Pd: coding sequence of the mature part of the
		glycolate dehydrogenase gene of Chlamydomonas
		reinhardtii (Nakamura et al., 2005), adapted to wheat
		codon usage
5294-5591	Clockwise	Tnos: sequence including the 3′ untranslated region of the
		nopaline synthase gene from the T-DNA of pTiT37
		(Depicker et al., 1982)
5668-6504	Clockwise	5668-6504 Clockwise P35S3: sequence including the
		promoter region of the Cauliflower Mosaic Virus 35S
		transcript (Odell et al., 1985)
6505-7056	Clockwise	bar: coding sequence of the phosphinothricin
		acetyltransferase gene of Streptomyces hygroscopicus
		(Thompson et al., 1987).
7057-7340	Clockwise	Tnos: sequence including the 3′ untranslated region of the
		nopaline synthase gene from the T-DNA of pTiT37
		(Depicker et al., 1982)
7437-7461		LB: left border repeat from the T-DNA of Agrobacterium
		tumefaciens (Zambryski, 1988)

TABLE 4
Description of the genetic elements of pTCD163.
Nt positions	Orientation	Description
1-25		RB: right border repeat from the T-DNA of Agrobacterium
		tumefaciens (Zambryski, 1988)
26-361	Counter	3′ ocs: sequence including the 3′ untranslated region of the
	Clockwise	octopine synthase gene of Agrobacterium tumefaciens (De
		Greve et al., 1982)
362-1540	Counter	sbpaseOs-1Pa: coding sequence of the sedoheptulose-1,7-
	Clockwise	bisphosphatase gene of Oryza sativa (rice) (Feng et al.,
		2007)
1541-2009	Counter	intron_act1Os: first intron of the actin 1 gene of Oryza
	Clockwise	sativa (rice) (Mc Elroy et al., 1990).
2010-4759	Counter	PrbcSOs: sequence including the promoter region of the
	Clockwise	ribulosebisphosphate carboxylase small subunit gene of
		Oryza sativa (rice) (Nomura et al., 2000)
4760-4959	Counter	3′35S: sequence including the 3′ untranslated region of the
	clockwise	35S transcript of the Cauliflower Mosaic Virus (Sanfacon
		et al., 1991)
4960-7733	Clockwise	PrbcSOs: sequence including the promoter region of the
		ribulosebisphosphate carboxylase small subunit gene of
		Oryza sativa (rice) (Nomura et al., 2000)
7734-8202	Clockwise	intron_act1Os: first intron of the actin 1 gene of Oryza
		sativa (rice) (Mc Elroy et al., 1990).
8203-8574	Clockwise	TPotpC-1Pm: coding sequence of the optimized transit
		peptide, containing sequence of the RuBisCO small subunit
		genes of Zea mays (corn) and Helianthus annuus
		(sunflower) (Lebrun et al., 1996), adapted to wheat codon
		usage
8575-11754	Clockwise	gdhCrh-2Pd: coding sequence of the mature part of the
		glycolate dehydrogenase gene of Chlamydomonas
		reinhardtii (Nakamura et al., 2005), adapted to wheat
		codon usage
11755-12052	Clockwise	3′ nos: sequence including the 3′ untranslated region of the
		nopaline synthase gene from the T-DNA of pTiT37
		(Depicker et al., 1982)
12129-12965	Clockwise	P35S3: sequence including the promoter region of the
		Cauliflower Mosaic Virus 35S transcript (Odell et al.,
		1985)
12966-13517	Clockwise	bar: coding sequence of the phosphinothricin
		acetyltransferase gene of Streptomyces hygroscopicus
		(Thompson et al., 1987).
13518-13801	Clockwise	3′ nos: sequence including the 3′ untranslated region of the
		nopaline synthase gene from the T-DNA of pTiT37
		(Depicker et al., 1982)
13898-13922		LB: left border repeat from the T-DNA of Agrobacterium
		tumefaciens (Zambryski, 1988)

REFERENCES

• Carrington & Freed 1990 Journal of Virology 64(4): 1590-1597
• Christou et al., 1991 Plant Mol Biol. 35(1-2): 197-203
• Clemente et al, 2000 Crop Sci. 40:797
• Dc Greve et al., 1982 Journal of Molecular and Applied Genetics 1: 499-511
• de Pater et al., 1992 Plant J. 2:837-844
• Depicker et al., 1982 Journal of Molecular and Applied Genetics, 1: 561-573
• Droste et al, 2002 Euphytica. 2002; 127:367-376
• Feng et al., 2007 Plant Cell Rep 26: 1635-1646
• Hiei et al., 1994 The Plant Journal 6(2): 271-282
• Hiei et al., 1997 Plant Mol Biol. 35: 205-218
• Kyozuka J. et al., 1993 Plant Physiology 102: 991-1000
• Lebrun et al., 1996 U.S. Pat. No. 5,510,471
• Maughan et al, 1999 In Vitro Cell. Dev. Biol Plant. 1999; 35:344-349
• McElroy D. et al., 1990 The Plant Cell, 2: 163-171
• Nakamura et al., 2005 Canadian Journal of Botany, 83: 820-833
• Nomura et al., 2000 Plant Molecular Biology, 44(1): 99-106
• Odell et al., 1985 Nature, 313: 810-812
• Olhoft & Somers, 2001 Plant Cell Rep, 20: 706-711
• Olhoft et al, 2001 Plant Cell Rep, 20: 731-737
• Sanfaçon et al., 1991 Genes and Development 5: 141-149
• Santarem & Finer, 1999 In Vitro Cellular and Developmental Biology—Plant. 35:451-455
• Si et al., 2003 Acta Botanica Sinica, 45(3): 359-364
• Thompson et al., 1987 EMBO Journal, 6: 2519-2523
• Trick & Finer, 1998 In Vitro Cellular and Developmental Biology—Plant 35:57-60
• Zambryski, 1988 Annual Review of Genetics, 22: 1-30
• Zhang et al, 1999 Plant Cell Tiss. Organ Cult. 56:37

Claims

1. (canceled)

2. A method for improving the assimilation of carbon in a plant comprising the steps of:

a. inserting into the genome of a plant a nucleic acid encoding an enzyme comprising sedoheptulose 1,7-bisphosphatase activity operably linked to a plant-expressible promoter, and optionally to a 3′ end region involved in transcription termination and/or polyadenylation; and

b. inserting into the genome of a plant one or more nucleic acids encoding one or more polypeptides having the enzymatic activity of glycolate dehydrogenase operably linked to a plant-expressible promoter, and optionally to a 3′ end region involved in transcription termination and/or polyadenylation, wherein said one or more polypeptides are located in chloroplasts of said plants.

3. The method of claim 2, wherein said enzyme comprising sedoheptulose 1,7-bisphosphatase activity further comprises FBPase activity.

4. The method of claim 2, wherein said enzyme comprising sedoheptulose 1,7-bisphosphatase activity is from a source heterologous with respect to the plant into which the nucleic acid is inserted, a plant species heterologous with respect to the plant into which the structural nucleic acid is inserted, from Oryza sativa, or encoded by a nucleic acid wherein the codon usage has been adapted to the codon usage in wheat.

5. (canceled)

6. (canceled)

7. (canceled)

8. (canceled)

9. (canceled)

10. (canceled)

11. The method according to claim 2, wherein said promoter includes the promoter region of the ribulose-bisphosphate carboxylase small subunit gene of Oryza sativa.

12. The method according to claim 11, wherein said promoter comprises the complement of the nucleotide sequence of SEQ ID No. 12 from nucleotide position 2010 to 4759.

13. The method according to claim 2, wherein said enzyme comprising sedoheptulose 1,7-bisphosphatase activity further comprises an amino acid sequence targeting said enzyme comprising sedoheptulose 1,7-bisphosphatase activity to the chloroplast or the chloroplast membrane or wherein said nucleic acid encoding an enzyme comprising sedoheptulose 1,7-bisphosphatase activity is integrated in the chloroplast genome of cells of said plant.

14. (canceled)

15. (canceled)

16. The method according to claim 2, wherein said one or more polypeptides having the enzymatic activity of a glycolate dehydrogenase are derived from the E. coli glc operon, a Chlamydomonas or Synechocystis glycolate dehydrogenase, or an Arabidopsis glycolate dehydrogenase.

17. The method according to claim 16, wherein said one or more polypeptides comprise an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID No. 3, SEQ ID No. 4, and SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID NO. 8, or SEQ ID No. 9.

18. (canceled)

19. The method according to claim 16, wherein said polypeptides form a multi-subunit fusion protein.

20. (canceled)

21. (canceled)

22. (canceled)

23. (canceled)

24. (canceled)

25. (canceled)

26. The method according to claim 16, wherein said one or more polypeptides having glycolate dehydrogenase activity further comprise an amino acid sequence targeting said enzyme or subunits to the chloroplast or the chloroplast membrane or wherein said one or more nucleic acids encoding one or more polypeptides having glycolate dehydrogenase activity are integrated in the chloroplast genome of cells of said plant.

27. (canceled)

28. (canceled)

29. The method according to claim 2, wherein said promoter includes the promoter region of the ribulose-bisphosphate carboxylase small subunit gene of Oryza sativa or the promoter region of the cytosolic fructose 1,6 bisphosphatase gene of Oryza sativa.

30. The method according to claim 29, wherein said promoter comprises the complement of the nucleotide sequence of SEQ ID No. 12 from nucleotide position 2010 to 4759 or the nucleotide sequence of SEQ ID No. 11 from nucleotide position 75 to 1272.

31. (canceled)

32. (canceled)

33. (canceled)

34. (canceled)

35. (canceled)

36. (canceled)

37. (canceled)

38. (canceled)

39. (canceled)

40. (canceled)

41. (canceled)

42. (canceled)

43. (canceled)

44. (canceled)

45. (canceled)

46. A plant cell, a plant part, a plant or a seed comprising in its genome a nucleic acid encoding an enzyme comprising sedoheptulose 1,7-bisphosphatase activity operably linked to a plant-expressible promoter, and optionally to a 3′ end region involved in transcription termination and/or polyadenylation; and one or more nucleic acids encoding one or more polypeptides having the enzymatic activity of glycolate dehydrogenase operably linked to a plant-expressible promoter, and optionally to a 3′ end region involved in transcription termination and/or polyadenylation, wherein said one or more polypeptides are located in chloroplasts of said plants.

47. The plant cell, a plant part, a plant or a seed of claim 46, wherein said enzyme comprising sedoheptulose 1,7-bisphosphate activity further comprises FBPase activity.

48. The plant cell, a plant part, a plant or a seed of claim 46, wherein said enzyme comprising sedoheptulose 1,7-bisphosphatase activity is from a source heterologous with respect to the plant into which the nucleic acid is inserted, a plant species heterologous with respect to the plant into which the structural nucleic acid is inserted, from Oryza sativa, or encoded by a nucleic acid wherein the codon usage has been adapted to the codon usage in wheat.

49. (canceled)

50. (canceled)

51. (canceled)

52. (canceled)

53. (canceled)

54. (canceled)

55. The plant cell, a plant part, a plant or a seed of claim 46, wherein said promoter includes the promoter region of the ribulose-bisphosphate carboxylase small subunit gene of Oryza sativa.

56. The plant cell, a plant part, a plant or a seed of claim 55, wherein said promoter comprises the complement of the nucleotide sequence of SEQ ID No. 12 from nucleotide position 2010 to 4759.

57. The plant cell, a plant part, a plant or a seed according to claim 46, wherein said enzyme comprising sedoheptulose 1,7-bisphosphatase activity further comprises an amino acid sequence targeting said enzyme comprising sedoheptulose 1,7-bisphosphatase activity to the chloroplast or the chloroplast membrane or wherein nucleic acid encoding an enzyme comprising sedoheptulose 1,7-bisphosphatase activity is integrated in the chloroplast genome of cells of said plant.

58. (canceled)

59. (canceled)

60. The plant cell, a plant part, a plant or a seed according to claim 46, wherein said one or more polypeptides having the enzymatic activity of a glycolate dehydrogenase are derived from the E. coli glc operon, a Chlamydomonas or Synechocystis glycolate dehydrogenase, or an Arabidopsis glycolate dehydrogenase.

61. The plant cell, a plant part, a plant or a seed according to claim 60, wherein said one or more polypeptides comprise an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID No. 3, SEQ ID No 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID NO. 8, or SEQ ID No. 9.

62. (canceled)

63. The plant cell, a plant part, a plant or a seed according to claim 60, wherein said polypeptides form a multi-subunit fusion protein.

64. (canceled)

65. (canceled)

66. (canceled)

67. (canceled)

68. (canceled)

69. (canceled)

70. The plant cell, a plant part, a plant or a seed according to claim 60, wherein said one or more polypeptides having glycolate dehydrogenase activity further comprise an amino acid sequence targeting said enzyme or subunits to the chloroplast or the chloroplast membrane or wherein said one or more nucleic acids encoding one or more polypeptides having glycolate dehydrogenase activity are integrated in the chloroplast genome of cells of said plant.

71. (canceled)

72. (canceled)

73. The plant cell, a plant part, a plant or a seed according to claim 46, wherein said promoter includes the promoter region of the ribulose-bisphosphate carboxylase small subunit gene of Oryza sativa or the promoter region of the cytosolic fructose 1,6 bisphosphatase gene of Oryza sativa.

74. The plant cell, a plant part, a plant or a seed according to claim 73, wherein said promoter comprises the complement of the nucleotide sequence of SEQ ID No. 12 from nucleotide position 2010 to 4759 or the nucleotide sequence of SEQ ID No. 11 from nucleotide position 75 to 1272.

75. (canceled)

76. (canceled)

77. (canceled)

78. (canceled)

79. (canceled)

80. (canceled)

81. (canceled)

82. (canceled)

83. (canceled)

84. (canceled)

85. (canceled)

86. (canceled)

87. (canceled)

88. (canceled)

89. (canceled)

90. A food product obtained from a plant or plant part or seed of claim 46.

91. (canceled)