Graphene coated particles, their method of manufacture, and use
Disclosed is a composition of matter comprising a biologically active substance bound to a graphene-coated dielectric-core particle, and methods for making and using the same.
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This application is a Divisional of U.S. application Ser. No. 15/932,667 (currently pending), which is the national phase application of PCT application PCT/US/2017/000014, Filed 15 Feb. 2017, which claims priority to provisional application 62/296,537, filed 17 Feb. 2016, all of whose contents of which are incorporated herein in their entirety.
TECHNICAL FIELDThe following inventive concepts relate to particles coated with graphene having chemical groups attached, their manufacture, and use.
BACKGROUND ARTGraphene is a form of carbon characterized by a flat hexagonal aromatic lattice of carbon atoms. Graphene, applied to a substrate in a single layer or sheet, is seeing increasing scientific use a basic material in industrial production and scientific research, due to its interesting electrical and physical properties. However, there are limits to its current use. Primarily, it is difficult to both apply chemical and electrical functionalities to bulk graphene and have that “activated” graphene attached to a usable substrate for distribution. Therefore, a need exists to provide mechanisms to create and distribute such particles.
Further, delivering biologically active substances to targets is an ever-present problem in the pharmaceutical, herbicide, pesticide, fertilizers, fungicide, and water treatment industries, amongst others. Therefore, a solution to this problem is also sought.
DISCLOSURE OF INVENTIONEmbodiments of the present invention may provide for a graphene coated, chemically active particle comprising a silica core, a graphene layer surrounding the core, and negatively charged moieties or basic moieties attached to the outer surface of the graphene layer.
Embodiments may also provide for a method for creating a biologically active graphene based substrate as provided above, additionally comprising binding a biologically active peptide to a metal ion, the method comprising adding graphite to a solution of weak base, neutralizing the solution with a weak acid substance, super-heating the solution, then immersing a metal in an electrolyte solution with a dissimilar metal, neutralizing the electrolyte solution, adding a polypeptide to the metal electrolyte solution, and combining the peptide/electrolyte solution with the graphene solution.
Embodiments may also provide for a method to bind biologically active molecules and organisms in-situ to graphene coated charged particles, comprising spraying or soaking the chemically active graphite particle solutions described above over in-situ biological molecules or organisms.
Embodiments of the present invention can provide for a method of manufacturing a charged, graphene coated particle comprising: adding graphite to a weak basic solution, neutralizing the solution with a weak acid substance, immersing a metal in an electrolyte solution with a dissimilar metal, combining the graphite and metal solutions, adding a silica substrate to the mixed solution, and super-heating the mixed solution.
The figures herein are schematic, and are not necessarily to scale. Features may be exaggerated for simplicity or to assist understanding. Skilled practitioners will recognize that some components or steps may have been omitted for simplicity, and that other components or steps may be added without deviating from the underlying concepts disclosed herein.
The inventive concepts disclosed herein are described with respect to particular example embodiments and processes along with some explanations for their operation, however, one skilled in the art will recognize that the underlying principles disclosed may be embodied in other examples or methods not necessarily identical to the given examples. Therefore, the limits of the inventive concepts are to be taken as the claimed subject matter, and not the individual examples themselves.
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The core material used herein is an inert material that exhibits dielectric properties. More particularly, in some embodiments, the core particle 102 may be a silica (or mostly silica) structure such as finely divided and screened silica, diatomaceous earth, coal, or other similar largely inert material. The chemically active group 106, such as the e.g. negatively charged or basic group may be evenly or unevenly distributed over the graphene shell 104 (depicted here as a layer due to the relatively small nature of the groups). The physical structure of the core material may be non-uniform and may contain multiple flat surfaces where the surfaces are multi angled, and/or multi-dimensional. In some embodiments, the negatively charged or basic group (chemically active group 106) may be a hydroxy group. The construction of graphene particle 100 will be discussed below with regard to
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In some embodiments, the complex 114 can be produced by combining the metal-attached polypeptide 108, or charged cell 118, together with the chemically active graphene complex 100, each of which holds respective ionic charges: 112 (a positive charge or metal ion, such as one or more of the transition metals of manganese, iron, copper, zinc, etc.) and 106 (a negative charge or base, such as a sodium, potassium, or ammonium base salts) that are attracted towards one another.
More specifically, in some embodiments, the positively charged metal ion 112—which gives the polypeptide 110 located on charged cell containing organism 116 (or overall, the charged cell 118 possessing polypeptide 110) its charge—binds at the active site[s] (“receptors”) of the polypeptide 110. Meanwhile, the negatively charged/basic surface coating 106 on the charged graphene complex 100 is a chemical component attached to graphene shell 104 of graphene particle 100. These two components attract each other to form overall complex 114.
In some embodiments, there may be a multiplicity of metal-bound polypeptides 108 or charged cells 118 complexed with each graphene coated particle 100. In other embodiments, each charged cell 118 may have multiple graphene coated particles 100 complexed with it. The distribution of the chemically graphene complexes 100 over the surface of a charged cell 118 may be uneven, depending on the attraction between the polypeptide 110 located on the single charged cell 118 and the charged graphene complex 100, and on the physical location of metal-attached polypeptides over the surface of the cell. A process of producing the final complex 114 is described in
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More specifically, in some embodiments, the core particle 102 may be any type of inert, dielectric material and may include, for example, silica, coal, or diatomaceous earth. At step 604, in some embodiments, the graphite can be milled to 0.5 to 10 micron sized particles. In some embodiments, at step 606 the pulverized graphite can be immersed with the core particles in a reducing solution of sodium hydroxide diluted to 5 to 10 mol, and combined with ammonia. The immersion time of this step may be about 10 minutes to an hour. In some embodiments, the solution is cooled overnight at room temperature in a water bath, dry-ice or a dry-ice/acetone bath to control the temperature of the reaction. After the immersion mixture is cooled to room temperature, nitrogen gas may be bubbled into the solution used to reduce any resulting oxygen.
In some embodiments, at step 608, the mixture's pH can then be neutralized or adjusted to a pH of 6.0-7.0 by a sodium or potassium base salt or ammonium salt, or the like, thus producing a hydroxide ion that imparts a negative charge to the graphene. The mixture is then superheated in step 614 to produce the charged graphene-core particle complex 100. The heating method may be a microwave or other heat source.
The result of example process 600 is a core particle coated with graphene having negatively charged or basic groups attached: a charged graphene coated particle complex 100. Without being limited by theory, the charged graphene coated particle complex 100 resulting from step 614 may comprise of a core 102 that is coated with a graphene shell 104 that carries for example, a basic hydroxy group 106 that may attract the positively charged metal ion(s) 112 of the charged polypeptide or single-celled organism 108. This graphene coated complex 100 is capable of holding a negative electrical or chemical charge or basic group 106, and has the capacity to attract, bind and potentially become a carrier for a positively charged (or metal bound) polypeptide 108 or single-celled organism 118.
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More specifically, in some embodiments, in step 712, an electrolyte solution is produced. The dilute electrolyte solution may be made with a ratio of 1:10 up to 1:100 acid to water. The acid may be phosphoric, sulfuric, hydriodic, or hydrochloric acid, or a weaker acid such as citric or acetic acid, or a combination of acids (see Table 1, below). The pH of the electrolyte solution may be under 4.0. At step 714, two metals of dissimilar electrical potential and biological importance, such as transitional metals like Cu and Mn may be dissolved in the electrolyte solution. The metals added during step 714 are in the form of large particles, or are milled, ground, or screened 1 to 10 micron or larger sized particles. After the metals are dissolved in the electrolyte solution, the solution's pH may be raised to about 6-7 in step 716, by adding a sodium or potassium base salt (such as sodium hydroxide), or the like in step 714, which produces a positively charged metal ion solution. The poly peptide 110 or single-celled organism 116 is then imbued with charged metal ion 112 by immersing a solution of the polypeptide in the metal ion solution in step 718. In alternative embodiments, step 718 may be carried out with a suspension of single celled organism 118 having on them polypeptides 110 that are capable of binding a charged metal ion. Without being bound by theory, the resulting complex produced in step 718 can be a charged polypeptide or single celled organism 108, which may comprise of a polypeptide 110 or single celled organism 116 with a with a positively charged ion 112 attached to it at a receptor site.
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In alternative embodiments, complex 114 may be produced in an alternative way. Before being added to the ploy-peptide 110 or single-celled organisms 116 at step 718, the electrolyte solution prepared in process 720 may instead be added directly to the graphene particles produced as a result of process 600. This produces a composition consisting on a graphene coated particle with a basic surface coating which is in turn bound to the metal ions of the solution prepared in 720. This new mixture may be sprayed, soaked or otherwise applied to the environment to bind to polypeptides 110 or single celled organisms 116 (such as mold spores) in the environment in-situ, producing complex 114. If the mixing is performed in environmental water, this may result in a purification of the water.
It has been observed that creating the complex 114 in-situ as described above causes mold spores, bacteria, and other microscopic biologicals to aggregate and come out of suspension in air and water, making it substantially easier to clean or disinfect surfaces.
Furthermore, it has been observed that spraying the solution of complex 114 results in a surface coating of the complex 114 on the target of spraying or immersing that is both even and resistant to removal. Thus, creating and applying a complex of 114 wherein the polypeptide 110 has biologic activity (such as a pesticide) may be an effective mechanism of distributing such biologically active polypeptide and effectuating its purpose.
Claims
1. A graphene coated particle comprising an inert core, on which sits a graphene coating, which graphene coating has negatively charged or basic groups on its outer surface.
2. The composition according to claim 1 additionally comprising metal ions bound to the negatively charged or basic groups.
3. The composition according to claim 2 additionally comprising a polypeptide bound to the metal ions.
Type: Application
Filed: Jul 29, 2019
Publication Date: Jan 2, 2020
Applicant: Millman Systems LLC (Harwood, MD)
Inventor: Stephen Jeffery Gorton (Rowlett, TX)
Application Number: 16/602,047