SINGLE- AND MIXED-METAL NANOPARTICLES, NANOPARTICLE CONJUGATES, DEVICES FOR MAKING NANOPARTICLES, AND RELATED METHODS OF USE
Nanoparticles, nanoparticle conjugates, devices for making nanoparticles and nanoparticle conjugates, and related methods of use and synthesis are described.
This application claims the benefit of the U.S. Provisional Patent Application No. 62/576,009 filed Oct. 23, 2017, the disclosure of which is incorporated herein by reference in its entirety.
BACKGROUNDMany current solution-phase, single- and mixed-metal nanoparticle production protocols are based on seed-mediated, “bottom-up” self-assembly of sub-nanometer metal building blocks. The mechanisms governing seed-mediated growth of gold nanoparticles in solution phase at standard ambient temperature and pressure (SATP) are thought dependent on two pathways: kinetic controls and selective surface passivation. The latter matches various combinations of reductants and surfactants with specific metal chemistry and ion concentration. Development of various protocols based on these principles has expedited development of directed synthesis of monometallic nanoparticles. Given the different physico-chemical properties of multiple metals, e.g., finding matching surfactants and suitable reaction environments that work in concert, producing metal nanoparticles with well-defined morphology and composition is, however, disproportionately challenging.
In conventional, i.e. SATP, solution-phase reaction environments, trace elements, solvated gasses, volatile compounds, and potentially reactive micro-contaminants are already present or enter as ambient contamination. Residual polymers and surfactants are found in and on nanoparticles synthesized via this path; their synthetic role is difficult to define and post-production removal often difficult, if not impossible. They typically remain unidentified in reactions carried out at SATP. Given that even the most seemingly innocuous micro-contaminants influence nucleation and growth, i.e., below ppm concentration, a contaminant-free system is important for production of crystalline metal nanoparticles. Surfactant- and contaminant-free metal nanoparticle sols are needed for the increased demand from combined targeted imaging and therapeutic applications.
SUMMARYThis summary is provided to introduce a selection of concepts in a simplified form that are further described below in the Detailed Description. This summary is not intended to identify key features of the claimed subject matter, nor is it intended to be used as an aid in determining the scope of the claimed subject matter.
In one aspect, the present disclosure provides a nanoparticle comprising a solid metal core that is free or substantially free of ligands or non-metallic bonds. In an embodiment, the nanoparticles described herein further include an organic shell coupled to an outer surface of the solid metal core.
In another aspect, the present disclosure provides a method for making metal nanoparticles that have a solid metal core that is free or substantially free of ligands and non-metallic bonds.
In another aspect, the present disclosure provides device for low-pressure, solution-phase synthesis of metal nanoparticles. In an embodiment, the device includes a single Schlenk line assembly; and an all-glass chemical reaction environment coupled to the single Schlenk line assembly including: a vacuum-stopped back pressure overload valve; a plurality of back pressure-loaded injectors; and a reaction vessel. In an embodiment, the device further includes a manometer-gated, tri-valved high-vacuum interface coupled to the single Schlenk line.
In another aspect, the present disclosure provides a method of tagging a target nucleic acid substrate with a nanoparticle as described further herein, comprising assembling a targeted nanoparticle construct on the target nucleic acid substrate, and a targeting moiety coupled thereto, wherein the targeting moiety selectively binds to a sequence in the target nucleic acid substrate.
The foregoing aspects and many of the attendant advantages of the claimed subject matter will become more readily appreciated as the same become better understood by reference to the following detailed description, when taken in conjunction with the accompanying drawings, wherein:
Nanoparticles, nanoparticle conjugates, devices for making nanoparticles and nanoparticle conjugates, and related methods of use are described.
NanoparticlesIn one aspect, the present disclosure provides a nanoparticle including a solid metal core free or substantially free of ligands and non-metallic bonds. The absence or substantial absence can be verified by mass spectral analysis, such as, gas chromatography-mass spectrometry, inductively coupled plasma-mass spectroscopy, and the like.
The core of the nanoparticles described herein is a solid metal core. In an embodiment, the solid metal core comprises a metal chosen from an alkali metal, an alkaline earth metal, a transition metal, a post-transition metal, a lanthanide metal, an actinide metal, and a metalloid. In an embodiment, the solid metal core comprises a plurality of metal atoms of a single element. In an embodiment, the solid metal core is a mixed metal core comprising two or more metal elements. In an embodiment, the mixed metal core has a predetermined ratio of components, dependent upon, for example, reaction conditions used in their synthesis.
In an embodiment, the nanoparticles described herein have a well-defined polyhedral geometry (in the sense that they are essentially free of or free of lattice defects). The absence or substantial absence of lattice defects can be verified by, for example, high-resolution scanning tunneling electron microscopy. In an embodiment, the nanoparticles described herein have essentially no or no surface ligands that are bound to a surface of the solid metal core. As described further herein with respect to the methods and devices of the present disclosure, in an embodiment, the nanoparticles are synthesized in reactions free or substantially free of surfactants, trace elements, solvated gasses, volatile compounds and potentially reactive micro-contaminants. The absence or substantial absence of surfactants, trace elements, solvated gases, volatile compounds, and potentially reactive micro-contaminants can be verified by mass spectral analysis, such as, gas chromatography-mass spectrometry, inductively coupled plasma-mass spectroscopy, and the like. Accordingly, in an embodiment, the nanoparticles are free of such contaminants. Further, in an embodiment, the solid metal core does not include non-metallic elements. In this regard, the solid metal core does not include non-metallic bridges between metal atoms disposed in the solid metal core.
In an embodiment, the nanoparticles described herein have a diameter in a range of about 0.5 nm to about 1000 nm. In an embodiment, the nanoparticles described herein have a diameter in a range of about 2 nm to about 200 nm. In an embodiment, the nanoparticles described herein have a diameter in a range of about 4 nm to about 100 nm.
In an embodiment, the nanoparticles described herein may be characterized by their three-dimensional geometry or shape. In an embodiment, the nanoparticles have a polyhedral shape. In an embodiment, the polyhedral shape includes Platonic solids, such as pyramids, and including Bravais-lattices face-centered cubic (FCC), hexagonal close packed (HCP), and body-centered cubic (BCC). In an embodiment, the nanoparticles described herein are supra-atomic crystalline materials exhibiting a Bravais-lattice selected from the group consisting of FCC, HCP and BCC. In an embodiment, the nanoparticles described herein have structure selected from the group consisting of possess a tetragonal, pyramidal, and hexagonal pyramidal structure.
In an embodiment, the nanoparticles described herein are electron-dense. Electron density of the nanoparticles of the present disclosure can be measured by back-scattered electrons scattered off of the nanoparticles. In an embodiment, the nanoparticles described herein are not hollow and/or electron-dense.
The solid metal core can have any number of physical characteristics. In an embodiment, one or more of elements of the solid metal core is radioactive. In an embodiment, the radioactive element is chosen from a radio-isotope of gold, indium, gadolinium, and platinum. In an embodiment, the solid metal core includes isotopes of alkali, alkaline earth, post-transition, transition, and lanthanide metals. In this regard, the nanoparticle is capable of delivering a radio-isotopic payload to a target tissue or cell. Likewise, such a radioactive nanoparticle can be used, for example, for labelling or imaging of a sample. In an embodiment, the solid metal core includes a stable isotope of iron, silver, or gold.
A number of such radioactive nanoparticles are illustrated in
Such nanoparticles can be used, for example, targeted imaging, diagnostics, and cancer therapies.
In an embodiment, the solid metal core is magnetic. As discussed further herein with respect to the methods of the present disclosure, nanoparticles comprising such magnetic solid metal cores are capable being magnetophoresced or otherwise isolated from a solution or suspension. In this regard, they are suitable in concentrating a target from a complex mixture. In an embodiment, the solid metal core has a magnetic property chosen from ferromagnetism, paramagnetism, and super-paramagnetism. In an embodiment, the solid metal core comprises one or more magnetic elements chosen from cobalt, iron, nickel, manganese, europium, and combinations thereof.
As discussed further herein, in an embodiment, the magnetic nanoparticle further includes a targeting moiety and can be used to manipulate DNA and extract specific nucleic acid fragments for sequencing and or genetic manipulation. In a further embodiment, the targeted magnetic nanoparticle includes a luminescent intercalating/cutting platinum compound configured to increase its functional spectrum.
In an embodiment, the solid metal core is configured to generate one or more signals in response to absorbing or scattering electromagnetic radiation. In an embodiment, the solid metal core is fluorescent. In an embodiment, the solid metal core is luminescent. In an embodiment, the solid metal core generates a signal including a visible light signal in response to absorbing or scattering electromagnetic radiation. In an embodiment, the solid metal core includes a material selected from the group consisting of gold, silver, iron, platinum, europium, and combinations thereof.
In an embodiment, the nanoparticles described herein are configured to generate a detectable signal in response to absorbing or scattering radio-frequency electromagnetic radiation. In an embodiment, the nanoparticles described herein are configured to amplify or concentrate radio-frequency electromagnetic radiation. In an embodiment, the solid metal core includes a metal selected from the group consisting of iron, gold, and an alloy of platinum and silver. In this regard, the nanoparticles described herein are suitable as antennae to focus radio-frequency delivery onto a target, particularly when the nanoparticles are targeted to a particular tissue or cell, such as in cancer treatment.
In an embodiment, the nanoparticles described herein include an organic shell coupled to an outer surface of the solid metal core. In an embodiment, the organic shell is coupled directly to the solid metal core. In an embodiment, the organic shell fully covers or envelopes the solid metal core. In an embodiment, the organic shell partially covers the solid metal core. In an embodiment, there is no linker between the organic shell and the solid metal core. In an embodiment, the outer surface of the solid metal core has a zero valence state. In an embodiment, the outer surface of the solid metal core coupled to the organic shell does not include metal oxides.
In an embodiment, the nanoparticles described herein further include secondary shell coupled to an outer surface of the organic shell. In an embodiment, the secondary shell comprises one or more metals. In an embodiment, the secondary shell completely covers or envelopes the organic shell. In an embodiment, the nanoparticles described herein include tertiary, quaternary, and more shells disposed on or over the organic layer.
In an embodiment, the secondary shell includes a plurality of metal nanoparticles coupled to an outer surface of the organic shell. In the illustrated embodiment of
In an embodiment, the nanoparticles described herein include nanoparticle conjugates. In an embodiment, the organic shell includes an organic component coupled to the outer surface of the solid metal core chosen from a restriction enzyme, a protein, a carbohydrate, a lipid, an amino acid, a nucleic acid, and combinations thereof.
In an embodiment, the organic shell includes a targeting moiety configured to selectively bind to a target substrate. In an embodiment, the targeting moiety includes components chosen from one or more of an antibody, a functional antibody fragment or derivative, a TALENS, an aptamer, a restriction enzyme, a nucleic-acid guided endonuclease, an exonuclease, a polymerase, a nucleic acid modifying protein, an antibody, and combinations thereof. In an embodiment, the targeting moiety is chosen from Cas-9 and dCas-9. In an embodiment, the targeting moiety includes an endonuclease coupled to a guide nucleic acid, such as a guide RNA, configured to selectively bind to a target nucleic acid.
In an embodiment, the organic shell includes a topoisomerase. In an embodiment, the topoisomerase is configured to selectively bind DNA.
In an embodiment, the organic shell includes a restriction enzyme. In an embodiment, the restriction enzyme is configured to selectively bind to DNA.
In an embodiment, the organic shell includes an endonuclease.
In an embodiment, the organic shell includes an antibody, such as a monoclonal antibody.
In an embodiment, the targeting moiety includes an RGD peptide. In an embodiment, the RGD peptide is a linear RGD peptide. In an embodiment, the RGD peptide is a cyclic RGD peptide. Embodiments of a nanoparticle according to the present disclosure including an RGD peptide are illustrated in
In an embodiment, the organic shell includes an anti-oxidant component. In a further embodiment, the nanoparticle including the anti-oxidant component further includes a targeting moiety, as discussed further herein, configured to selectively bind to a target. In this regard, in such an embodiment the targeted anti-oxidant particle can be used to selectively bind to, image, and destroy diseased cells, such as cancer cells.
Device for Making Nanoparticles and Nanoparticle ConjugatesConventional solution-phase manufacture of single- and mixed-metal nanoparticles, core-shell nanoparticles, polymer and polymer-encapsulated metal nanoparticles is currently carried out under aerobic or inert atmospheres. Solution-phase nanoparticle synthesis under low or partial-pressure atmospheres has been described. Anoxic metal nanoparticle synthesis generally prevents metal-oxygen-metal bonding (“MOM”) bridges and generally favors metal-metal bond formation. However, conventional protocols and equipment do not permit in-line synthesis, surface derivatization, and conjugation of such nanoparticles under constant partial pressure and under inert atmosphere.
Specifically, certain previous devices used for low-pressure, solution-phase synthesis of single- and mixed-metal nanoparticles included vapor-phase ballasts. In low-pressure, solution-phase chemistry, reactants may be suspended in a low-pressure “fog” at or below ambient temperature. Regulation of equilibrated vacuum levels between vapor-phase ballasts and the reaction environment is needed to establish and maintain a solution-vapor phase equilibrium between the reaction vessel and the ballasts. This has been referred to as an external “extended headspace”. Previous designs using vapor-phase ballasts required an additional set of high-vacuum glassware ballasts; replenishing their liquid contents, continuous monitoring, and measuring and adjusting vacuum levels between ballasts and reaction vessels in-real time. This involved human intervention since complete automation of this process was not possible. Additional adjunct elements were also needed including, for example, a second Schlenk line and, thus, additional liquid nitrogen traps and gated manometers bridging vapor-phase ballasts to the reaction vessel. Those designs including vapor-phase ballasts provided surfaces separate from the reaction vessel onto which reactants condense. This involved disassembly and exhaustive cleaning of the entire Schlenk line(s) prior to successive reactions, polymer or metal shell deposition and/or conjugations.
To better meet this challenge, in an aspect the present disclosure provides a device for making, for example, the nanoparticles described herein. In an embodiment, the device includes a high-vacuum, all-glass reaction environment. A high-vacuum, all-glass environment provides good conditions for solution-phase syntheses of metal nanoparticles, because it can be cleaned with, for example, hydrofluoric acid to remove trace elements prior to high vacuum-evacuation of volatile compounds. To establish and maintain a suitable reaction environment for solution-phase growth of crystalline nanoparticles at reduced pressure, liquid-vapor boundary cryotechnology was combined with cleanroom and fabrication technology drawn from the semiconductor industry.
Accordingly, in an embodiment, the device includes a low-pressure, solution-phase single Schlenk line. In that regard, attention is directed to
High-vacuum and solution-phase chemistry are in principle incompatible. Accordingly, in an embodiment, the device 100 includes a manometer-gated, tri-valved high-vacuum interface 106 coupled to the single Schlenk line 102 configured to provide an extended headspace maintained under low-pressure to establish a vapor-phase gradient in the reaction environment 126. In an embodiment, the device 100 for low-pressure, solution-phase synthesis of metal nanoparticles includes a single Schlenk line assembly 102; and an all-glass chemical reaction environment 126 coupled to the single Schlenk line assembly 102. In an embodiment, the all-glass chemical reaction environment 126 coupled to the single Schlenk line assembly 102 includes one or more vacuum-stopped back-pressure overload valves 112A and 112B; a plurality of back pressure-loaded injectors 114A and 114B; and a reaction vessel 120.
In an embodiment, the manometer-gated, tri-valved high-vacuum interface 106 is configured to establish and maintain a consistent and precise vacuum level within the single Schlenk line 102. Constant low pressure may be maintained by a wet/dry turbo-molecular pumping system (HVAC). Pressure gated isolation of individual environments may be balanced through the tri-valved gate 106 in concert with vacuum-stopped back-pressure overload valves 112A and 112B and back-pressure-loaded injectors 114A and 114B. In an embodiment, the manometer-gated, tri-valved high-vacuum interface 106 is in selective fluidic communication with a source of noble gas, such as noble gas sources 116A-116C, and a vacuum 104; and further comprises a manometer-gated switch 124 configured to selectively place the single Schlenk line 102 in fluid communication with one of the sources of noble gas 116A-116C and the vacuum 124. In this regard, the manometer-gated, tri-valved high-vacuum interface 106 is configured to balance pressure at the vapor-phase by using the gated-manometer 124 to switch between vacuum 104 and noble gas sources 116A-116C routed through the single Schlenk line 102 and its components without the use of an external “headspace” that was established with bridged vapor phase ballasts as in previous instruments, e.g., dual Schlenk line devices. Accordingly, in an embodiment, the device does not include a vapor-phase ballast.
As illustrated in
In an embodiment, one or more components of the reaction environment 126 are coupled to the single Schlenk line by one or more ultra-high vacuum-sealed joints, such as gated manometers 118A and 118B.
The devices 100 described herein permit in-line, sequential layering of composite (organic and/or polymer) shells and deposition/fixation of secondary nanoparticles. This process can be further augmented for rapid, scalable nano-composite production; including conjugation of targeting molecules (e.g., IgGs), signal-generating moieties (fluorophores, radioisotopes) as well as visible (e.g., color-coded materials) and their encapsulation into organic shells (e.g., for advanced drug-delivery systems), without any modifications to the design. The single Schlenk-line design is configured for true sealed-system “daisy-chain” production of multiplexed nanoparticles. Such a device 100 conforms to current ISO/GLP/GMP medical and pharmaceutical standards and includes nanoparticles produced for diagnostic and therapeutic applications superseding existing ISO and GMP standards for biomedical research.
Methods for Using the Device to Make Nanoparticles and Nanoparticle ConjugatesIn another aspect, the present disclosure provides a method for making the nanoparticles and nanoparticle conjugates described herein.
The methods described herein incorporate two (2) unbridged manometer-gated, VSBPO valves on a single Schlenk vacuum line. See for example,
The configurations described herein further allow continuous layering of organic polymers and/or simultaneous conjugation of ligands, proteins or nucleic acids. Previously-described methods for low-pressure, solution-phase production of nanoparticles required a series of vapor-phase ballasts, where continuous injection required an interrupted batching process. In contrast, the methods described herein facilitate linear synthetic progressions, i.e., back-and-forth synthesis using a preliminary product that can act as a secondary physical platform. This can be through controlled deconstruction of one core-shell composite into another with the addition of secondary or tertiary reagents. For example, magnetic nanoparticle DNA composites can be separated into two moieties; the magnetic moiety separated and removed and the DNA/protein composite to be processed further.
Single Schlenk line synthesis, as described herein, permits in-line sequential layering of composite, such as organic shells, and deposition/fixation of secondary nanoparticles. This process can be further augmented for rapid scalable nano-composite production; including conjugation of targeting molecules (e.g., IgGs), signal generating moieties (fluorophores, radioisotopes), as well as visible (e.g., color-coded materials) and their encapsulation into organic envelopes (e.g., for advanced drug-delivery systems), without any modifications to the design. The single Schlenk design is suitable for true “daisy-chain” production of multiplexed nanoparticles.
Methods for Using Nanoparticle ConjugatesNanoparticles such as those disclosed herein can be incorporated into a variety of applications, all of which are encompassed by the present disclosure. Inclusion of an appropriate targeting moiety with the nanoparticle provides a targeted nanoparticle construct that can selectively bind any target substrate of interest. In one embodiment, the targeting moiety is configured to specifically bind to a target nucleic acid sequence, which permits the nanoparticle tagging of a nucleic acid substrate that comprises the target nucleic acid sequence. This can be applied for a variety of applications, including use in targeted nucleic acid sequencing applications, identification and/or isolation of nucleic acids from any source organism or synthetic system and/or fragments thereof, sequencing-based assaying technologies in vitro and/or in vivo. Furthermore, the targeted nanoparticle conjugates can be used in imaging and diagnostics. For example, the targeted nanoparticle conjugates can be configured for use in commercial research and development, such flow and mass cytometry, microscopy, and spectroscopy applications.
Accordingly, in one embodiment the disclosure provides a method of tagging a target nucleic acid substrate with a nanoparticle. The method comprises assembling a targeted nanoparticle construct on the target nucleic acid substrate. The targeted nanoparticle construct comprises a nanoparticle, for example as described herein, and a targeting moiety coupled thereto. The targeting moiety selectively binds to a sequence in the target nucleic acid substrate.
The term “assembling a targeted nanoparticle construct” encompasses embodiments where the targeted nanoparticle construct is pre-assembled and is merely contacted to the target nucleic acid substrate. This pre-assembly can comprise coupling the nanoparticle to the nanoparticle targeting moiety or a precursor thereof and then contacting the targeted nanoparticle construct to the target nucleic acid substrate under conditions to permit selective binding of the targeting moiety to the sequence in the target nucleic acid substrate. See, e.g., the illustrative schematic representations of
Alternatively, the nanoparticle targeting moiety can be first contacted to the target nucleic acid substrate under conditions to permit selective binding of the targeting moiety to the sequence in the target nucleic acid substrate. Once bound, the nanoparticle can then be added and coupled to the targeting moiety already bound to the sequence in the target nucleic acid substrate. See, e.g., the illustrative schematic representation of
In
Coupling of the nanoparticle to the targeting moiety can be accomplished using appropriate reducing agents. A non-limiting list of exemplary reducing agents useful to conjugate the nanoparticle and targeting moiety (e.g., DNA-interacting proteins such as Cas9) includes TCEP (tris(2-carboxyethyl)phosphine), EDC or EDAC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride), a metal, ADH, alcohol dehydrogenase, ascorbic acid, bis(pinacolato)diboron, a borane, catecholborane, carrots, copper hydride, copper (low valent), chromium (low valent), daucus carota, decaborane, DEMS, DIBAL-H, diborane, diethoxymethylsilane, diethyl 1,4-dihydro-2,6-dimethyl-3,5-pyridinedicarboxylate, diisobutylaluminium hydride, diisopropylaminoborane, 1,3-dimethylimidazol-2-ylidene borane, dimethyl sulfide borane, DMSB, formaldehyde, formic acid, hantzsch ester, hydrazine, hydrogen, indium (low valent), iron, isopropanol, LAH, LiTEBH, lithium, lithium aluminum hydride, lithium tetrahydridoaluminate, lithium triethylborohydride, magnesium, manganese, mercaptoethylamine-HCl, 3-mercaptopropionic acid, 2-methylpyridine borane, 3-MPA, NBSH, neodymium (low valent), nickel, nickel borohydride, niobium (low valent), 2-nitrobenzenesulfonylhydrazide, phenylsilane, phosphorous acid, PICB, a-picoline-borane, pinacolborane, PMHS, polymethylhydrosiloxane, potassium, potassium borohydride, potassium iodide, potassium tetrahydroborate, 2-propanol, red-Al, rongalite, samarium (low valent), Schwartz's reagent, a silane, sodium, sodium bis(2-methoxyethoxy)aluminumhydride, sodium bisulfite, sodium borohydride, sodium cyanoborohydride, sodium dithionite, sodium hydrogensulfite, sodium hydrosulfite, sodium hydroxymethanesulfinate, sodium tetrahydroborate, sodium triacetoxyborohydride, strontium, sulfur, superhydride, tetramethyldisiloxane, a tin hydride, titanium (low valent), TMDSO, tributylstannane, tributyltin hydride, tributylphosphine, trichlorosilane, triethylphosphine, trimethylphoshpine, triphenylphosphine, triphenylphosphite, triethylsilane, tris(trimethylsilyl)silane, TTMSS, vasicine, vitamin C, zinc, and zirconocene chloride hydride.
Target SubstratesTarget substrates generally comprise nucleic acids. As used herein, the term “nucleic acid” refers to any polymeric molecule that comprises multiple nucleotide subunits (i.e., a polynucleotide). Nucleic acids encompassed by the present disclosure can include deoxyribonucleotide polymer (DNA), ribonucleotide polymer (RNA), cDNA or a synthetic nucleic acid known in the art.
Nucleotide subunits of the nucleic acid polymers can be naturally occurring or artificial or modified. A nucleotide typically contains a nucleobase, a sugar, and at least one phosphate group. The nucleobase is typically heterocyclic. Canonical nucleobases include purines and pyrimidines and more specifically adenine (A), guanine (G), thymine (T) (or typically in RNA, uracil (U) instead of thymine (T)), and cytosine (C)). The sugar is typically a pentose sugar. Suitable sugars include, but are not limited to, ribose and deoxyribose. The nucleotide is typically a ribonucleotide or deoxyribonucleotide. The nucleotide typically contains a monophosphate, diphosphate, or triphosphate. These are generally referred to herein as nucleotides or nucleotide residues to indicate the subunit. Without specific identification, the general terms nucleotides, nucleotide residues, and the like, are not intended to imply any specific structure or identity. The nucleotides can also be synthetic or modified.
In some embodiments, the target substrates can include substrates comprising DNA. Such DNA-based substrates can have DNA that is derived from any relevant source, such as bacteria, archaea, eukaryotic microorganisms, single eukaryotic cells, synthetic organisms, organelles such as mitochondria, DNA viruses, transposable elements and other genomic elements, circulating tumor cells. The DNA substrates can be obtained from or derived from biological samples, such as bodily fluids and biopsies, or from cell-free DNA (cfDNA), treated samples. The DNA can be naturally derived or synthetic. The synthetic DNA can include PNAs and LNAs, and the like. The DNA substrate can be in processed or pre-treated samples, such as DNA that has been converted into a library or library pool for next generation sequencing, nucleic acid polymers. The target DNA substrate can be attached to a solid surface and/or further comprise additional non-DNA components. For example the DNA substrate can comprise the target DNA and a protein or peptide (including fusion a protein), an aptamer, a fusion protein, RNA, PNA, and the like. The treated sample can have the target DNA substrate preserved in situ, such as in formalin-fixed paraffin embedded (FFPE) tissue samples. Furthermore the target DNA substrate can comprise the target DNA sequence non-covalently bound (i.e., hybridized) to another nucleic acid molecule, such as DNA or RNA. The DNA can be produced by reverse transcription from an RNA template.
The DNA component of the target substrate can be of any appreciable size such that a targeting moiety can selectively bind to it. In some embodiments, the DNA component is at least 4 bases in length or more (e.g., up to a million bases or even more—there is no upper limit). The DNA can be linear, circular, branched, single stranded, partially or fully double stranded, and the like. The DNA component can have multiple domains, which are of known or unknown sequence, so long as there is sufficient known sequence and/or three-dimensional structure to permit the development of a targeting moiety to be configured to selectively bind to it. For example, a domain of unknown sequence can be flanked on one or both of the 5′ end and 3′ end with domains of known sequence. The 5′ end and 3′ end with domains of known sequence can be, for example, adaptor and/or barcode or tag sequences that facilitate capture, amplification, and/or sequencing, etc., using e.g., PCR, qPCR, next generation sequencing platforms, hybrid capture, hybridization chain reaction, and the like. Alternatively, a known sequence can be flanked on one or both of the 5′ end and 3′ end with domains of unknown sequence. In one illustrative example, a circular cloning plasmid with an insert may have multiple domains of known sequence that flank either end of an insert with unknown sequence. In any embodiment, the known or unknown domains can be DNA, or can alternatively be another nucleic acid such as RNA and/or PNA, as long as there is a known DNA sequence that is targeted by the targeting moiety for selective binding.
In other embodiments, the target substrates can include substrates comprising RNA. The target RNA can be derived from total RNA, mRNA, siRNA, microRNA, long non-coding RNA, and the like. Like DNA targets substrates, the target RNA substrates can have RNA that is derived from any relevant source, such as bacteria, archaea, eukaryotic microorganisms, single eukaryotic cells, synthetic organisms, organelles such as mitochondria, RNA viruses, circulating tumor cells. The RNA substrates can be obtained from or derived from biological samples, such as bodily fluids and biopsies, or from cell-free RNA (cfRNA), treated samples. The RNA substrate can be in processed or pre-treated samples, such as RNA that has been converted into a library or library pool for next generation sequencing, nucleic acid polymers. The target DNA substrate can be attached to a solid surface and/or further comprise additional non-DNA components. For example the DNA substrate can comprise the target DNA and a protein or peptide (including fusion a protein), an aptamer, a fusion protein, DNA, PNA, and the like. The treated sample can have the target RNA substrate preserved in situ, such as in formalin-fixed paraffin embedded (FFPE) tissue samples. Furthermore the target RNA substrate can comprise the target RNA sequence non-covalently bound (i.e., hybridized) to another nucleic acid molecule, such as RNA or DNA.
The description of the DNA component of DNA-containing substrates can be generally applicable to the RNA component of a substrates comprising RNA and are not repeated in their entirety. However, it is briefly noted that the RNA component can similarly be of any size and can contain various domains of known and unknown sequence in any configuration. There can also be domains of different nucleic acid types, such as
The target nucleic acid substrates can be in any relevant biological sample, such as a fluid or solid sample obtained from a subject. Such fluid samples include blood, plasma, mucus, cerebral spinal fluid, and the like. Solid samples include biopsy samples. The target nucleic acid substrates can be in a prepared or processed sample (e.g., ultimately derived from a subject). For example, cells may have been grown ex vivo for some time and then processed to release the cellular DNA. Finally, solid tissue or cell samples can be processed and preserved to permit in situ applications for microscopic imaging. Such samples mentioned herein are for illustrative purposes only and other samples relevant for this disclosure are known in the art.
Targeting MoietiesThe targeting moieties can be any molecule or molecule subassembly that can selectively target and bind a target substrate of interest with a specific affinity (i.e., detectable over background). As used herein, the term “selectively bind” means the ability to form a covalent or non-covalent interaction that the targeting moiety and target substrate form a complex under conditions where the targeting moiety does not interact and form complexes with off-target substrates (i.e., with divergent structures). Typically, the non-covalent interaction is sufficiently strong such that the complex has prolonged stability, e.g., to permit isolation and other manipulations.
In some embodiments, the targeting moiety is a nucleic acid-interacting protein. Illustrative, non-limiting examples of nucleic acid-interacting proteins useful as a targeting moiety include enzymatic proteins such as type II restriction enzymes, DNA/RNA modifying proteins, RNA/DNA guided endonucleases, endonucleases, exonucleases, fusion proteins, TALENS, antibodies and functional fragments or derivatives thereof, aptamers, peptides, and the like.
In some embodiments, the targeting moiety is an antibody. As used herein, the term “antibody” can refer to whole antibodies, but also encompasses antibody fragments and derivatives thereof, even if not explicitly indicated as such. The antibodies (and fragments or derivatives) can be obtained or derived from any antibody-producing mammal (e.g., mouse, rat, rabbit, and primate including human), that specifically bind to an antigen of interest. Exemplary antibodies include polyclonal, monoclonal and recombinant antibodies; multispecific antibodies (e.g., bispecific antibodies); humanized antibodies; murine antibodies; chimeric, mouse-human, mouse-primate, primate-human monoclonal antibodies; and anti-idiotype antibodies. The antigen-binding molecule can be any intact antibody molecule or fragment thereof (e.g., with a functional antigen-binding domain).
An antibody fragment is a portion derived from or related to a full-length antibody. A “functional” fragment typically refers to the maintenance of the ability to bind the target substrate. Thus, the antibody fragment preferably includes the complementarity-determining regions (CDRs), antigen binding regions, or variable regions thereof. Illustrative examples of antibody fragments useful in the present disclosure include Fab, Fab′, F(ab)2, F(ab′)2 and Fv fragments, scFv fragments, diabodies, linear antibodies, single-chain antibody molecules, multispecific antibodies formed from antibody fragments, and the like. A “single-chain Fv” or “scFv” antibody fragment comprises the VH and VL domains of an antibody, wherein these domains are present in a single polypeptide chain. The Fv polypeptide can further comprise a polypeptide linker between the VH and VL domains, which enables the scFv to form the desired structure for antigen binding. Antibody fragments can be produced recombinantly, or through enzymatic digestion.
Antibodies can be further modified to suit various uses. For example, a “chimeric antibody” is a recombinant protein that contains the variable domains and complementarity-determining regions (CDRs) derived from a non-human species (e.g., rodent) antibody, while the remainder of the antibody molecule is derived from a human antibody. A “humanized antibody” is a chimeric antibody that comprises a minimal sequence that conforms to specific complementarity-determining regions derived from non-human immunoglobulin that is transplanted into a human antibody framework. Humanized antibodies are typically recombinant proteins in which only the antibody complementarity-determining regions (CDRs) are of non-human origin.
Production of antibodies can be accomplished using any technique commonly known in the art. For example, the production of a polyclonal antibody can be accomplished by administering an immunogen containing the antigen of interest to an antibody-producing animal. For example, the antigen of interest (also referred to as “target antigen”) can be administered to a mammal (e.g., a rat, a mouse, a rabbit, a chicken, cattle, a monkey, a pig, a horse, a sheep, a goat, a dog, a cat, a guinea pig, a hamster) or a bird (e.g., a chicken) so as to induce production of a serum containing an antigen-specific polyclonal antibody. The target antigen can be administered in combination with other components known to facilitate induction of a B-cell response, such as any appropriate adjuvant known in the art. Furthermore, the polyclonal antibody reagent can be further processed to remove or subtract any antibody members that have unacceptable affinity for antigens that are not the antigen of interest. The resulting polyclonal antibody reagent will exhibit enhanced specificity for the antigen of interest and are useful for detection and quantification purposes. Many approaches for adsorption of polyclonal antibody reagents to reduce cross-reactivity exist, are familiar to persons of ordinary skill in the art, and are encompassed by the present disclosure.
Monoclonal antibodies can be prepared using a wide variety of techniques known in the art including the use of hybridoma, recombinant, and phage display technologies, or a combination thereof. For example, monoclonal antibodies can be produced using hybridoma techniques including those known in the art and taught, for example, in Harlow et al., Antibodies: A Laboratory Manual, (Cold Spring Harbor Laboratory Press, 2nd ed. 1988); Hammerling et al., in: Monoclonal Antibodies and T-Cell Hybridomas 563-681 (Elsevier, N.Y., 1981), incorporated herein by reference in their entireties. The term “monoclonal antibody” refers to an antibody that is derived from a single clone, including any eukaryotic, prokaryotic, or phage clone, and not the method by which it is produced. Methods for producing and screening for specific antibodies using hybridoma technology are routine and well known in the art.
Antibody fragments that recognize specific target substrate sequences or structures can be generated by any technique known to those of skill in the art. For example, Fab and F(ab′)2 fragments of the invention can be produced by proteolytic cleavage of immunoglobulin molecules, using enzymes such as papain (to produce Fab fragments) or pepsin (to produce F(ab′)2 fragments). F(ab′)2 fragments contain the variable region, the light chain constant region and the CHI domain of the heavy chain. Further, the antibodies of the present invention can also be generated using various phage display methods known in the art.
As used herein, the term “aptamer” refers to oligonucleic or peptide molecules that can bind to specific target substrates of interest. Nucleic acid aptamers usually are short strands of oligonucleotides that exhibit specific binding properties to target nucleic acids (or other antigens). They are typically produced through several rounds of in vitro selection or systematic evolution by exponential enrichment protocols to select for the best binding properties, including avidity and selectivity. One type of useful nucleic acid aptamers are thioaptamers, in which some or all of the non-bridging oxygen atoms of phophodiester bonds have been replaced with sulfur atoms, which increases binding energies with proteins and slows degradation caused by nuclease enzymes. In some embodiments, nucleic acid aptamers contain modified bases that possess altered side-chains that can facilitate the aptamer/target binding.
Peptide aptamers are protein molecules that often contain a peptide loop attached at both ends to a protamersein scaffold. The loop typically has between 10 and 20 amino acids long, and the scaffold is typically any protein that is soluble and compact. One example of the protein scaffold is Thioredoxin-A, wherein the loop structure can be inserted within the reducing active site. Peptide aptamers can be generated/selected from various types of libraries, such as phage display, mRNA display, ribosome display, bacterial display and yeast display libraries.
In some embodiments, the nucleic acid-interactive protein has or can incorporate a guide nucleic acid that confers target substrate specificity. For example, Cas9, dCas-9, or other RNA/DNA guided endonucleases known in the art can be loaded with guide RNAs that confer specificity for a specific nucleic acid sequence within a target substrate. Typically, the guide RNA will have sequence complementarity with the sequence contained in the target nucleic acid substrate. The guided endonucleases can be loaded with the guide RNA(s) in a guide loading buffer at a temperature range of 4-37° C. for 1-15 min. As indicated above, the loading of the guide RNA can be performed prior to or after the coupling of the nanoparticle to the endonuclease.
The guide RNA can be constructed following published protocols. Specifically, a protospacer adjacent motif (PAM) site is identified and the flanking sequence (target sequence) is chosen to minimize off target genomic sites. The target sequence is concatenated to published guide-RNA hairpin sequences. The final guide-RNA (gRNA) can be synthesized individually by commercial oligonucleotide synthesis vendors. A pool of gRNAs can be synthesized by commercial oligonucleotide synthesis vendors to produce a pool of gRNAs in a single tube. Additionally, a DNA oligonucleotide compliment to the gRNA sequence can be synthesized with an added T7 or sp6 recognition site for an in vitro transcription reaction to produce the gRNA molecules. This can be performed for individual gRNAs or with pools of DNA complements of gRNAs. The nucleic acids can be exotic nucleic acids or contain modifications (for secondary/tertiary attachment). Non-limiting, exemplary modifications to the gRNA include the addition of one or more of biotin, biotin (azide), biotin-TEG, dual biotin, PC biotin, desthiobiotin-TEG, azide (NETS ester), acrydite, digoxigenin (NETS ester), cholesteryl-TEG, I-linker™, amino group, hexynyl, octadiynyl dU, thiol, dithiol, thiol modifier C6 S—S, PNA, locked nucleic acids, iso-G/iso-C, and the like, or any combination thereof.
Detection of Target Nucleic AcidsIn some embodiments, the method further comprises imaging the targeted nanoparticle construct on the target nucleic acid substrate. Such embodiments can be performed, for example on preserved tissue samples for in situ imaging, where the targeted nanoparticle constructs provide for NP-based imaging of bound target nucleic acid substrates. For example, the presence of specific target RNA transcripts can be mapped in a particular region or the presence of a genomic element of a pathogen can be revealed among host tissue. The imaging can be the product of properties of the bi-metallic nanoparticles themselves or imaging moieties loaded thereon. Furthermore, the presences of the bound nanoparticles can be used as a quantifiable signal to infer the amount or concentration of the bound substrate, for example in color-shift assays used on a membrane or in solution for diagnostic purposes.
In some embodiments, after the targeted nanoparticle construct is assembled on the target nucleic acid substrate, unbound targeted nanoparticle constructs, or unassembled subcomponents thereof, are washed away to avoid background signal.
Enrichment of Target Nucleic AcidsIn further applications, the targeted nanoparticle construct can be used to enrich, isolate, and/or purify nucleic acids molecules of interest from an initial milieu of sample components (e.g., from cellular components and/or non-target nucleic acid molecules). After enrichment, isolation, and/or purification, the target nucleic acids can be subjected to further analysis or manipulation. For example, the nucleic acids can be subjected to sequencing on any relevant platform to accomplish targeted sequencing. This can include such applications as exome sequencing, amplicon sequencing, assessing copy number variation, targeted bisulfide sequencing, large and small nucleic acid fragment sequencing on any platform (e.g., using PacBio, Illumina, nanopore technologies, and the like), mitochondrial sequencing, use of diagnostics and genotyping panels for specific genetic markers, forensics sequencing, pathogen and viral marker panels, assessing food and water quality using panel sequencing, and the like.
In applications where enrichment of the target nucleic acid is desired, unbound substrate and/or unbound components of the sample milieu are separated from the complexes of the targeted nanoparticle construct and target nucleic acid constructs.
In some embodiments, the complexes of the targeted nanoparticle construct and target nucleic acid constructs are immobilized while the unbound components of the sample are washed away. For example, in embodiments where the nanoparticles have magnetic metal components, a magnetic field can be applied to immobilize the substrate complexes during washing procedures. Removal of the magnetic field allows the complexes to go back into solution. The targeted nanoparticle construct can be separated from the target nucleic acid substrate using sample conditions (e.g., raised temperature, known denaturing buffers, and the like).
Exemplary loading buffer and wash solutions for the loading of guide RNA to the targeting moiety (e.g., Cas9) and for binding, washing, and isolating the target DNA substrate are listed in Table 1.
In other embodiments, buffer exchange and desalting columns can be used to enrich for the nanoparticle tagged nucleic acid substrates. Exemplary filters and columns include Amicon® Ultra Centrifugal Filters (Millipore) and Zeba spin columns (ThermoFisher)
It will be understood that the above embodiments, and other embodiments described herein, are provided for explanatory purposes, and are not intended to be limiting.
All publications, patents and patent applications cited herein are hereby expressly incorporated by reference for all purposes to the extent they are consistent with this disclosure.
Methods well-known to those skilled in the art can be used to construct primers and other synthetic nucleic acids, and other analytical tools such as expression vectors and recombinant bacterial cells according to this disclosure. These methods can include in vitro recombinant DNA techniques, synthetic techniques, conjugation techniques, and PCR techniques. See, for example, techniques as described in Maniatis et al., 1989, MOLECULAR CLONING: A LABORATORY MANUAL, Cold Spring Harbor Laboratory, New York; Ausubel et al., 1989, CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, Greene Publishing Associates and Wiley Interscience, New York, and PCR Protocols: A Guide to Methods and Applications (Innis et al., 1990, Academic Press, San Diego, Calif.).
EXAMPLESThe following Examples are illustrative of specific embodiments of the disclosure and various uses thereof. They are set forth for explanatory purposes only, and are not to be taken as limiting.
Example 1 Nanoparticle SynthesisPrimary Goethite synthesis was modified from historical protocols. The 50-100 nm nanoparticle precipitate extracted for analysis, washed then acidified with 1M HCl to re-solubilize iron. Transformation to hematite followed from recrystallization of Goethite. a) R. M. Cornell and U. Schwertmann in The Iron Oxides: Structure, Properties, Reactions, Occurrences and Uses, (Ed: C. Reinhardt), WILEY-VCH, Weinheim, 2003, pp. 61-64; b) Berger, et al., Journal of Chemical Education 1999, 76, 943; c) U. Schwertmann, E. Murad, Clays and Clay Minerals 1983; d) R. Massart, IEEE Trans. Magn. 1981, 17, 1247-1248; e) P. F. Hahn, Journal of Biological Chemistry 1946, 163, 435-435. Stage two synthesis begins with reverse-injection of 10 ml of the acidified iron solution from stage one into the second reaction solution of dilute chloroauric acid HAuCl4 2 nm iron clusters reassemble during initiation of stage two, forming the vertices of uniform trigonal plates. The AuFe NP composite reassembly process appears to follow LaMer kinetics with corollary reference to their recrystallization. a) A. Bee, R. Massart, S. Neveu, Journal of Magnetism and Magnetic Materials 1995, 149, 6-9; b) V. K. La Mer, R. H. Dinegar, J. Am. Chem. Soc. 1950, 72, 4847-4854. The stepwise evolution of higher order structures through coordinated bridging of iron vertices by sub-nanometer gold complexes, i.e., trigonal plates into a tetragonal prism, has been visualised and is supported by theoretical and experimental simulations. a) Yuk et al., Science 2012, 336, 61-64; b) Norris et al., Science 2008, 319, 1776-1779; c) A. Packter, Kolloid-Zeitschrift 1960, 170, 48-52; d) J. L. Frahn, Aust. J. Chem. 1958, 11, 399-405; e) H. V. Tartar, J. R. Lorah, J. Phys. Chem. 1924, 29, 792-798; d) M. Neidle, J. Am. Chem. Soc. 1917, 39, 2334-2350; f) A. Benrath, J. Prakt. Chem. 1917, 96, 190-201.
Example 2 Nanoparticle Synthesis Using the Devices of the Present DisclosureAll glassware was meticulously cleaned [i.e., HF acid cleaning and rinsed with milliQ water triple-distilled in glass. Liquids were degassed and sealed under Argon prior to assembly. Solids were dried and degassed and stored under high vacuum and all solutions prepared with water triple distilled in glass and exhaustively degassed prior to use. High-vacuum single-manifold Schlenk line was fitted with metal-to-glass seals and gated manometers; a motorized glass stirring rod was installed. Shaft speed was monitored at a constant 300 RPM by a non-contact RheinTacho digital tachometer. The temperatures of solutions were measured continuously with non-contact IR and glass thermometers immersed in silicon oil baths surrounding the reaction chamber. An Alcatel 2063 rotary-vane and Drytel 31 turbo-molecular vacuum pump were coupled via a triple-set switching valve to the LPSP Schlenk line fitted with primary and secondary LN2 gas traps. The full synthetic line was evacuated via flexible stainless steel hoses (1.5 in. i.d.) to 10−4 Pascal (Pa) and fluted with argon grade 6 thrice prior to each synthesis. Helium back-pressure loaded injection introduced liquids into a reaction chamber evacuated to <10−4 Pa, backfilled and maintained at 1-2 Pa.
Example 3 Characterization of Low Pressure Solution Phase Single Schlenk Line Synthesized Isotope-Doped Mixed-Metal Nanoparticles [LPSPSSL-MMNPS]Radioisotopes were incorporated into MMNP nanoparticles to act as radiotracers in the signal generating moiety of prototype nanoscale composites for use as in diagnostic and therapeutic applications. High-resolution electron microscopy, electron paramagnetic resonance (EPR), electron energy loss (EELS), and X-ray dispersive spectroscopy (XRDS) were used to determine composition, purity and structure of “cold” mixed-metal nanoparticles. Comprehensive analysis of individual LPSPSSL-MMNP preparations revealed highly uniform size, structure and composition of individual nanoparticle reaction products.
As shown in
Rapid LPSPSSL synthetic protocol incorporating Indium (111In) into a gold nanoparticle complex (See model in
Hydrodynamic size measurements showed narrowly distributed indium-111 labeled gold particles of 7-8 nm before and after purification. UV-visible absorbance spectra of the indium-111 labeled gold particles before and after purification showed a stable maximum at 524 nm. See
UV-visible absorbance spectroscopy of conjugated targeted of the distributed indium-111 labeled gold nanoparticles in serum reveals long-term stability. See
Gold-coated gadolinium, Au@68Gd, nanoparticles were synthesized via LPSPSSL protocol. Ultra-pure solutions of 5 nm particles were examined by UHR-TEM (See
Spectroscopy characterization of the Au@111In nanoparticles closely matches their TEM images and size distribution measurements. See
Purity was measured via analysis of thin-layer chromatography (Radio-TLC). See
An Indium-111 labeled gold nanoparticle platform, modified with the tumor targeting sequence arginine-glycine-aspartate (RGD), was developed and utilized for tumor cell targeting in-vitro and in-vivo studies. See generally
A modification of a two-step protocol (Grabarek and Gergely, 1990) for the activation of proteins with EDC/sulfo-NHS and subsequent conjugation with amine-containing compounds is illustrated in
DOTA-TATE and DOTA-TOC have 4 carboxyl groups, 3 of which are free to bind COOH-reactive fluorophores, e.g. hydrazines et al. AF350-hydrazide was chosen out of a wide array of COOH-reactive fluorophores due to low MW and putative heightened membrane permeability. EDC or EDAC, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide is used to facilitate and enhance this reaction.
Prepare:
a) 0.1M IVIES Buffer For 1 L: Dilute 19.5 Free IVIES acid into 500 ml millipure H2O under stirring. Bring final volume to 1 L and bring to pH6 with NaOH
b) Dilute 2 mg DOTA-TATE (or DOTA-TOC) into 1000 μl 0.1M MES Buffer. In other words, 2 mg/ml in a 1.5 ml Eppendorf
c) Fluorophore Preparation:
Make stock solution 55 mM of AF350 (or other AF-Hydrazine fluorophore) i.e., Place 1 mg AF350 Hydrazide MW 349.29 into 26 μl DMSO
d) Immediately prior to conjugation place 10 mg EDAC in 1 ml MES Buffer DOTA-TATE Fluorophore Conjugation:
1. Add 25 μl (c) Stock AF 350 sol'n. to (b) (1 ml DOTA-TATE/MES pH6)
2. Add 15 μl (d) Stock EDAC to above mix of AF 350 and DOTA-TATE/MES in Eppendorf
Cap Eppendorf and invert to mix at least 4×. Incubate 2-3 h RT.
A reaction schematic is illustrated in
Cells contacted with the nanoparticles of EXAMPLE 3 are depicted in
Two different toxicological assays are presented:
A. Ratiometric imaging of anti-oxidant Ro− GFP cells (
B. Ussing Chamber measurement of effect of DHLA/GSH delivery to primary tissue culture from ΔCFTR—[cystic fibrosis primary tissue] (
In assay A, cells were transformed incorporating a redox—(reduction/oxidation) sensitive probe, RoGFP. These cells were then exposed to a strong oxidant (peroxide). This is presented in
In assay B, primary (human) tissue culture was placed in multiwell Ussing Chambers (2×4) with a constant flow of phsiological buffer. The Ussing experimental apparatus enables real-time measurement of micro conductivity (charge), hence viability and function, of ion-channels across thousands of cells in ex vivo tissue over extended periods (days). Think of this as massive patch-clamp technology. The putative effect of antioxidants (AO) in cystic fibrosis therapy has been demonstrated (5). Lung tissue from CF patients ΔCFTR—are defective in Cl− (chloride) ion channel transport. Antioxidants open the defective channel temporarily. We wanted to see if by stabilizing the AO on the surface of nanoparticles we could amplify and extend the effect. Results in
Conclusions: The results of this Example indicate that nanoparticles surface stabilized with biological antioxidants provide a vector for therapies on a nontoxic nanoparticle platform.
1. Hanson et al. Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators. J Biol Chem (2004) vol. 279 pp. 13044-13053
2. Dooley et al. Imaging dynamic redox changes in mammalian cells with green fluorescent protein indicators. J Biol Chem (2004) vol. 279 pp. 22284-22293
3. Austin et al. Oxidizing potential of endosomes and lysosomes limits intracellular cleavage of disulfide-based antibody-drug conjugates. P Natl Acad Sci USA (2005) vol. 102 (50) pp. 17987-17992
4. Lohman and Remington. Development of a family of redox-sensitive green fluorescent protein indicators for use in relatively oxidizing subcellular environments. Biochemistry-Us (2008) vol. 47 (33) pp. 8678-8688
5. Schwarzer et al. Organelle redox of CF and CFTR-corrected airway epithelia. Free Radical Bio Med (2007) vol. 43 (2) pp. 300-316
Example 7 Quantitative Volumization of Micro-Metastatic Islets In A Mouse Cancer ModelPresently, there is a void in the detection in the micro-metastatic spread of many cancers. This is a lack of crucial information and influences the management decision of all metastatic cancers. Fluorescently tagged immuno-affinity probes targeted to a subtype of Somatostatin receptors on the surface of tumor cells (ATt20) were used to specifically detect putative cancer in micro-metastatic islets from a tumor in situ. Visualization and quantification of correlative confocal microscopy was used to determine specificity and sensitivity of probes.
Six-week old nude athymic mice were subcutaneously injected in the femoral region with cancer cells. In-vivo studies were performed when the xenografts had reached approximately 5 mm3. At this point, 1 MBq of purified tumor-targeted indium-111 labeled gold nanoparticle conjugates were injected via the tail vein. After 4 hours, mice were sacrificed and organs and tumors were removed and weighed. Untargeted indium-111 labeled gold nanoparticles served as negative controls. Imaging studies on micro-metastacies were performed 4 hours after injection.
A maximum intensity projection (MIP) is generated by standard “top-down” fluorescent imaging of a micro-metastatic islet (tumor). A MIP image provides an overview of internal and external volumes but only in two dimensions and each is projected on the other, and lacks spatial information.
Optical sections can be generated in X, Y and Z axis by confocal scanning laser microscopy (CSLM). Correlative mapping of each axis permits accurate localization and quantification of targeted fluorescent probes in situ.
With the application of modern microscopy software, a virtual light source can be generated to cast a shadow through all 3 axes producing a volumized “3D shadow projection.” See
Advanced software enables reconstruction of optical sections into a dynamic 3D model in real-time. This model can be sectioned at will, “sliced” (black planes) through any axis and its internal composition analyzed at the nanometer scale from point to point. We used commercial software, Imaris, from Bitplane, to produce these images.
Example 8 Targeted MMNP Nanoparticle UptakeTargeted MMNP nanoparticle uptake was studied in cell lines expressing various integrin levels. The high-integrin expressing human melanoma cell line, M21, was used along with low-integrin expressing M21-L cells, as a control cell line. In addition, an integrin-expressing human glioblastoma cell line, U87-MG, was used, and specific binding was tested with blocking experiments as controls.
Fluorescently tagged (AF-350) DOTA-TOC affinity conjugates were attached to 6 nm MMNP nanoparticles. These complexes were purified and incubated with ATt20 host cells for 4 hours (37° C., 5% CO2). See
Cells over-expressing Somatostatin sub-type were cultured directly on coated multi-well slides. Controls do not exhibit any penetration of nanoparticle complexes targeted to Somatostatins. Experimental samples exhibit blue “puff ball” clusters known to form from internalized surface receptors.
Example 9 MMNP-Labelled Enzymes Targeted to DNA Map Function to StructureThis Example describes examples of enzymes labelled with MMNPs of the present disclosure.
The rolling circle model of DNA replication in bacterial plasmids has been proposed for a long time.
Gold and iron nanoparticles were synthesized and bound to restriction enzymes and affinity ligands (here, monoclonal IgGs) to map sub-molecular domains on DNA and proteins in situ. See
5 nm gold NPs were bound to restriction enzymes BamH1 (
Tenascin (TNCN) is a six-armed macromolecule implicated in breast cancer. Sub-molecular Fibronectin type 3 domains (FNIII) were mapped on TNCN molecules with gold NP-labelled monoclonal antibodies (IgGs). IgGs binding the seventh FNIII domain on TNCN were produced and conjugated to 1 nm gold nanoparticles and incubated with TNCN. They were cryo-fixed and examined by ultra-high resolution in-lens scanning electron microscopy (modified Hitatchi S5000 UHR-SEM) with a YAG crystal back-scattered electron (BSE detection) that permitted mapping ultrastructural sub-domains within a sub-nanometer radius. See
A three-dimensional model of the monoclonal IgG bound to the TNCN molecule was reconstructed through multiple defocus micrograph sections. See
As shown in
This example describes an illustrative embodiment of the disclosed targeted nanoparticles applied to selectively bind and isolate a target nucleic acid molecule for further analysis.
Cas-9 (or dCas-9) protein is conjugated to a multi-metal nanoparticle (NP; as described above) in a reaction solution in the presence of a reducing agent. The conjugation reaction can be in a range of 1 min to 24 hours and at temperatures ranging from 4-37° C. The reaction is terminated by standard purification methods. Once purified, the conjugate complex can be used for targeting the substrate of interest after guide-RNA loading (gRNA) if using Cas-9, dCas-9. Alternative RNA- or DNA-guided endonuclease, or TALENS can also be employed in place of the Cas-9 or dCas-9.
The targeted NP complex is combined with the target substrate in a reaction buffer at a temperature range of 4-37° C. for 1-30 min. If the nanoparticle has an iron core, then the substrate:NP:dCAS-9 complex is immobilized with a magnet and the supernatant removed. The remaining substrate:NP:dCas-9 complex is re-suspended in a wash buffer, then immobilized and supernatant removed. This wash is optionally repeated, for example, three times. The final DNA can be removed from the dCas-9 (or other protein) by heat denaturation at 90-98° C. for 2-5 min or under pH 8-14. The resulting DNA product can be used as a substrate for PCR, qPCR, additional molecular modifications (blunt repair, A-tailing, ligation, etc.), next generation sequencing, microarray hybridization, and the like.
Example 12 Binding and Isolation of Target Nucleic Acids With A Multi-Metal Nanoparticle Composites of the Present DisclosureThe following example describes an illustrative embodiment of the disclosed targeted nanoparticles applied to selectively bind and isolate a target nucleic acid molecule for further analysis. In this embodiment, the nucleic acid-interacting protein (e.g., Cas-9 or dCas-9 protein) that specifically targets the nucleic acid is assembled with the multi-metal nanoparticle after it has selectively bound to the substrate nucleic acid of interest.
First, dCas-9 is loaded with gRNA's in a reaction buffer at a temperature range of 4-37° C. for 1-15 min. After gRNA loading, the substrate DNA/RNA (1 pg-10 μg) is added and dCas-9 locates the compliment of the loaded gRNA. This reaction occurs in a reaction buffer at a temperature range of 4-37° C. for 1-30 min. Once the reaction is completed, nanoparticles are added to the reaction followed by an addition of a reducing agent. The conjugation reaction can be as short as 1 min or as long as 24 hours and at temperatures ranging from 4-37 C. The reaction is terminated by standard purification methods or by nanoparticle immobilization if the nanoparticle has an iron core. If the particle has an iron core, then the substrate:NP:dCAS-9 complex is immobilized with a magnet and the supernatant removed. The remaining target substrate:NP:dCas-9 complex is re-suspended in a wash buffer, then immobilized and supernatant removed. This wash is repeated about three times. The final DNA can be removed from the dCas-9 (or other protein) by heat denaturation at 90-98° C. for 2-5 min or under pH 8-14. The resulting DNA product can be used as a substrate for PCR, qPCR, additional molecular modifications (blunt repair, A-tailing, ligation, etc.), next generation sequencing, microarray hybridization, and the like.
Example 13 Enrichment of Several Nucleic Acid Targets With Nanoparticles According to the Present DisclosureIn an illustrative assay three different DNA species were assayed in a single tube for targeted enrichment using the above approaches. Target DNA target 1 (4.5 kb), DNA target 2 (120 bp), and non-target DNA (120 bp) was added to the same tube. The two target DNAs were enriched using different targeted nanoparticle constructs incorporating (d)Cas-9 with guide RNAs that hybridize to different sequences within each of DNA target 1 and DNA target 2, respectively. The initial supernatant was collected when immobilizing the constructs with a magnetic field. This initial supernatant, as well as the supernatants collected during several rounds of washing, were used as template for PCR detection of the DNA constructs. The results are illustrated in
Quantitative PCR was performed on the DNA enriched for Target 1 DNA, Non-Target DNA, or their respective unbound pools and washing rounds. Two negative controls were performed that lacked input DNA. As illustrated in
As used herein, the singular forms “a”, “an”, and “the” include plural referents unless the context clearly dictates otherwise. For example, reference to a “nucleic acid” means one or more nucleic acids. It is generally noted that the use of the term “or” in the claims is used to mean “and/or” unless explicitly indicated to refer to alternatives only or the alternatives are mutually exclusive, although the disclosure supports a definition that refers to only alternatives and “and/or.”
Unless the context clearly requires otherwise, throughout the description and the claims, the words “comprise,” “comprising,” and the like are to be construed in an inclusive sense as opposed to an exclusive or exhaustive sense, such as in the sense of “including, but not limited to.” Words using the singular or plural number also include the plural and singular number, respectively. Additionally, the words “herein,” “above,” and “below,” and words of similar import, when used in this application, shall refer to this application as a whole and not to any particular portions of the application.
It is noted that terms like “preferably”, “commonly”, and “typically” are not utilized herein to limit the scope of the claimed invention or to imply that certain features are critical, essential, or even important to the structure or function of the claimed invention. Rather, these terms are merely intended to highlight alternative or additional features that can or cannot be utilized in a particular embodiment of the present invention.
For the purposes of describing and defining the present invention it is noted that the term “substantially” is utilized herein to represent the inherent degree of uncertainty that can be attributed to any quantitative comparison, value, measurement, or other representation. The term “substantially” is also utilized herein to represent the degree by which a quantitative representation can vary from a stated reference without resulting in a change in the basic function of the subject matter at issue. Words such as “about” and “approximately” imply minor variation around the stated value, usually within a standard margin of error, such as within 10% or in some cases 5% of the stated value.
Disclosed are materials, compositions, and components that can be used for, in conjunction with, and in preparation for the disclosed methods and compositions. It is understood that when combinations, subsets, interactions, groups, etc., of these materials are disclosed each of various individual and collective combinations is specifically contemplated, even though specific reference to each and every single combination and permutation of these compounds may not be explicitly disclosed. This concept applies to all aspects of this disclosure including, but not limited to, steps in the described methods and components of the described chamber or system. Thus, specific elements of any foregoing embodiments can be combined or substituted for elements in other embodiments. For example, if there are a variety of additional steps that can be performed, it is understood that each of these additional steps can be performed with any specific method steps or combination of method steps of the disclosed methods, and that each such combination or subset of combinations is specifically contemplated and should be considered disclosed. Additionally, it is understood that the embodiments described herein can be implemented using any suitable material such as those described elsewhere herein or as known in the art.
While illustrative embodiments have been illustrated and described, it will be appreciated that various changes can be made therein without departing from the spirit and scope of the invention.
Claims
1. A nanoparticle comprising a solid metal core free or substantially free of ligands and non-metallic bonds; and
2. The nanoparticle of claim 1, further comprising an organic shell coupled to an outer surface of the solid metal core.
3. The nanoparticle of claim 1, wherein the solid metal core comprises a metal chosen from an alkali metal, an alkaline earth metal, a transition metal, a post-transition metal, a lanthanide metal, an actinide metal, and a metalloid.
4. The nanoparticle of claim 1, wherein the solid metal core comprises a plurality of metal atoms of a single element.
5. The nanoparticle of claim 1, wherein the solid metal core is a mixed-metal core comprising two or more metal elements.
6. The nanoparticle of claim 5, wherein one or more of the two or more metal elements is radioactive.
7. The nanoparticle of claim 6, wherein the radioactive element is chosen from a radio-isotope of gold, indium, gadolinium, and platinum
8. The nanoparticle of claim 1, wherein the solid metal core is magnetic.
9. (canceled)
10. The nanoparticle of claim 8, wherein the solid metal core comprises one or more magnetic elements chosen from cobalt, iron, nickel, manganese, and europium.
11-12. (canceled)
13. The nanoparticle of claim 2, wherein the organic shell is coupled directly to the solid metal core.
14. The nanoparticle of claim 13, wherein there is no ligand between the organic shell and the solid metal core.
15. The nanoparticle of claim 13, wherein the outer surface of the solid metal core has a zero valence state.
16. The nanoparticle of claim 13, wherein the outer surface of the solid metal core does not include metal oxides.
17. The nanoparticle of claim 2, further comprising a secondary shell coupled to an outer surface of the organic shell.
18. (canceled)
19. The nanoparticle of claim 17, wherein the secondary shell further comprises a plurality of metal nanoparticles coupled to the outer surface of the organic shell.
20. The nanoparticle of claim 2, wherein the organic shell further comprises a targeting moiety coupled to the nanoparticle, wherein the targeting moiety is configured to selectively bind to a target substrate.
21. (canceled)
22. The nanoparticle of claim 20, wherein the targeting moiety includes components chosen from one or more of a restriction enzyme, an RNA/DNA modifying protein, an endonuclease, an RNA/DNA guided endonuclease, an exonuclease, a polymerase, a TALENS, an aptamer, an antibody, and a functional antibody fragment or derivative, Cas-9 and dCas-9.
23-24. (canceled)
25. The nanoparticle of claim 20, wherein the targeting moiety is coupled to the nanoparticle through a linker.
26-27. (canceled)
28. A device for low-pressure, solution-phase synthesis of metal nanoparticles, the device comprising:
- a single Schlenk line assembly; and
- an all-glass chemical reaction environment coupled to the single Schlenk line assembly including: a vacuum-stopped back pressure overload valve; a plurality of back pressure-loaded injectors; and a reaction vessel.
29-34. (canceled)
35. A method of tagging a target nucleic acid substrate with a nanoparticle, the method comprising assembling a targeted nanoparticle construct on the target nucleic acid substrate, wherein the targeted nanoparticle construct comprises a nanoparticle of claim 1 and a targeting moiety coupled thereto, wherein the targeting moiety selectively binds to a sequence in the target nucleic acid substrate.
36-52. (canceled)
Type: Application
Filed: Oct 23, 2018
Publication Date: Aug 13, 2020
Inventors: Joseph P. DUNHAM (Pasadena, CA), Marcus YAFFEE (Pasadena, CA)
Application Number: 16/758,770
