RELATED APPLICATIONS This application is a divisional of U.S. application Ser. No. 16/481,936, filed Jul. 30, 2019, which is a national stage filing under 35 U.S.C. 371 of International Patent Application Serial No. PCT/US2018/015819, filed Jan. 30, 2018, which claims priority under 35 U.S.C. § 119(e) to U.S. provisional patent application Ser. No. 62/452,409, filed Jan. 31, 2017, the entire contents of each of which are incorporated herein by reference.
REFERENCE TO A SEQUENCE LISTING SUBMITTED AS A TEXT FILE VIA EFS-WEB The instant application contains a Sequence Listing which has been submitted in ASCII format via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Apr. 5, 2022, is named L046170192US02-SEQ-JRV, and is 150,607 bytes in size.
FIELD The disclosure relates to methods, systems and kits for sequencing immune cell receptor repertoires from immune cells, such as T-cells or B-cells.
BACKGROUND Immune cell repertoires, such as B- or T-cell repertoires, consists of millions of lymphocytes, each expressing a different protein complex that enables specific recognition of a single antigen. CD4 and CD8 positive T-cells express so-called T-cell receptors (TCRs). These heterodimeric receptors recognize antigen-derived peptides displayed by major histocompatibility complex (MHC) molecules on the surface of antigen presenting cells, as described in Rudolph M G, Stanfield R L, Wilson I A. How TCRs bind MHCs, peptides, and coreceptors. Annu Rev Immunol. 2006; 24:419-66. TCRs are composed of two subunits, most commonly of one α and one β chain. A less common type of TCR contains one γ and one δ chain.
Alpha (α) chains consists of a (variable) V, a joining (J) and a constant (C) region, while beta (β) chains contain an additional diversity (D) region between the V and the J region (see FIG. 1), as described in Starr T K, Jameson S C, Hogquist K A. Positive and negative selection of T cells. Annu Rev Immunol. 2003; 21:139-76. Each of these TCR regions is encoded in several pieces, so-called gene segments, which are spatially segregated in the germline. In humans, the TCR α gene locus contains 54 different V gene segments, and 61 J gene segments. The human TCR β chain locus comprises 65 V, 2 D and 14 J segments. The great structural diversity of TCRs is achieved by somatic recombination of these TCR gene segments during lymphocyte development in the thymus. During this process, several gene segments of each region type are randomly selected and joined to form a rearranged TCR locus. Additional junctional diversity is created by the addition or removal of nucleotides at the sites of recombination, as described in Krangel M S. Mechanics of T cell receptor gene rearrangement. Curr Opin Immunol. 2009 April; 21(2):133-9. The process of V(D)J joining plays a critical role in shaping the third hypervariable loops (also called complementary determining regions, CDR3s) of the TCR α and β chains. These regions bind antigens and are essential for providing the high specificity of antigen recognition that TCRs exhibit.
Similarly to the TCR αβ, TCR gamma (γ) and delta (δ) segments undergo V(D)J rearrangement during thymus development. Both loci are recombined in the double negative (DN) stage of T-cell development. Differentiation towards γδ or αβ lineage relies on the ability of the cell to produce functional γδ or αβ TCR. The δ locus is embedded within the α locus. Dδ, Jδ and Cδ segments are located in between the V and the J segment of the α locus. The Vδ segments are the same as the Vα segments but only a fraction of the Vα segments are used for the TCR δ chain.
Overall, V(D)J recombination is able to generate millions of different TCR sequences and plays a critical role in an organism's ability to eliminate infections or transformed cells. Not surprisingly, TCR repertoires affect a wide range of diseases, including malignancy, autoimmune disorders and infectious diseases. TCR sequencing has been instrumental for our understanding of how the TCR repertoire evolves during infection or following treatment (e.g. after hematopoietic stem cell transplantation, chronical viral infection, immunotherapy). Further, the identification of TCRs on tumor-infiltrating lymphocytes and other T-cells that target cancer-specific epitopes has not only furthered our knowledge of malignant disease, but has also led to novel therapies for cancer such as adoptive T-cell transfer or cancer vaccines.
Due to the large diversity of sequences, determining TCR repertoires has been challenging in praxis. In the last couple of years, next generation sequencing (NGS) has opened up new opportunities to comprehensively assess the extreme diversity of TCR repertoires, as described in Genolet R, Stevenson B J, Farinelli L, Oster{dot over (a)}s M, Luescher I F. Highly diverse TCRα chain repertoire of pre-immune CD8+ T cells reveals new insights in gene recombination. EMBO J. 2012 Apr. 4; 31(7):1666-78; Robins H S, Campregher P V, Srivastava S K, Wacher A, Turtle C J, Kahsai O, Riddell S R, Warren E H, Carlson C S. Comprehensive assessment of T-cell receptor beta-chain diversity in alpha beta T cells. Blood. 2009 Nov. 5; 114(19):4099-107; Linnemann C, Heemskerk B, Kvistborg P, Kluin R J, Bolotin D A, Chen X, Bresser K, Nieuwland M, Schotte R, Michels S, Gomez-Eerland R, Jahn L, Hombrink P, Legrand N, Shu C J, Mamedov I Z, Velds A, Blank C U, Haanen J B, Turchaninova M A, Kerkhoven R M, Spits H, Hadrup S R, Heemskerk M H, Blankenstein T, Chudakov D M, Bendle G M, Schumacher T N. High-throughput identification of antigen-specific TCRs by TCR gene capture. Nat Med. 2013 November; 19(11):1534-41; Turchaninova M A, Britanova O V, Bolotin D A, Shugay M, Putintseva E V, Staroverov D B, Sharonov G, Shcherbo D, Zvyagin I V, Mamedov I Z, Linnemann C, Schumacher T N, Chudakov D M. Pairing of T-cell receptor chains via emulsion PCR. Eur J Immunol. 2013 September; 43(9):2507-15.
Since most current TCR sequencing techniques require enrichment of TCR genes for sequencing, the majority of methods include an amplification step, in which the nucleic acids encoding the individual TCRs are amplified. Therefore, one of the challenges of the TCR sequencing relates to the ability of the technology to maintain the proportion of each TCR during the amplification. Thus, the ways in which TCR libraries are prepared have a strong impact on the quality and the reliability of the obtained sequencing results and on the conclusions than can be drawn from the data. Several approaches have been used to amplify and sequence TCR repertoires in the past, each method with its own set of issues.
One frequently employed method for TCR sequencing is based on a multiplex PCR step, in which all the primers for the V and the J segments are mixed together to amplify all the possible V(D)J rearrangements/combinations, as described in Robins H S, Campregher P V, Srivastava S K, Wacher A, Turtle C J, Kahsai O, Riddell S R, Warren E H, Carlson C S. Comprehensive assessment of T-cell receptor beta-chain diversity in alpha beta T cells. Blood. 2009 Nov. 5; 114(19):4099-107. The main drawback of this technology is that the amplification is not quantitative: Because the efficiency of each primer pair varies, some TCR sequences are preferentially represented in the library.
Another TCR sequencing method uses a process called “DNA gene capture” to isolate TCR encoding DNA fragments, as described in Linnemann C, Heemskerk B, Kvistborg P, Kluin R J, Bolotin D A, Chen X, Bresser K, Nieuwland M, Schotte R, Michels S, Gomez-Eerland R, Jahn L, Hombrink P, Legrand N, Shu C J, Mamedov I Z, Velds A, Blank C U, Haanen J B, Turchaninova M A, Kerkhoven R M, Spits H, Hadrup S R, Heemskerk M H, Blankenstein T, Chudakov D M, Bendle G M, Schumacher T N. High-throughput identification of antigen-specific TCRs by TCR gene capture. Nat Med. 2013 November; 19(11):1534-41. However, since this method uses DNA rather than RNA, this method will also isolate V and J segments that have not yet undergone somatic rearrangement. As a consequence, many of the obtained sequencing data are uninformative for TCR gene identification as they do not contain the V(D)J region of rearranged TCR gene locus. Furthermore, using DNA instead of RNA for the TCR gene analysis may overestimate the diversity of the TCR repertoire as only one of the two β chains is expressed by the T-cells while the other gene is silenced (allelic exclusion).
A third method of TCR amplification is based on the 5′-Race PCR technology (SMARTer® Human TCR a/b Profiling Kit, Takara-Clontech). In this method, a nucleic acid adapter is added to the 5′-end of the cDNA during the reverse transcription step. As a result, TCR products can be subsequently amplified with a single primer pair, with one primer binding to the adapter at the 5′-end of the cDNA and the second primer binding to the constant region near the 3′-end of the cDNA. One of the disadvantages of this technique is that the amplification step will generate PCR fragments ranging between 500 and 600 bp. As the length of the V segment exceeds 400 bp it is actually not possible to sequence the V(D)J junction starting from the 5′end using ILLUMINA® sequencing technology, which can generate sequencing reads of up to 300 bp only. Sequencing of the V/J junction is thus usually performed from the constant region, crossing the J segment, the CDR3 region and part of the V segment. However, sequencing errors increase with the length of the sequencing read, and are thus most frequently introduced in the V segments—the region most challenging to correctly assign due to the high homology between different V segments. Consequently, sequencing starting from the constant region may lead to a reduction in the number of V segments that can be identified unambiguously. While this caveat can be avoided by paired-end sequencing, such modification of the protocol will significantly increase the duration and cost associated with this method.
SUMMARY With each of the current methods exhibiting significant shortcomings, there is thus a considerable need for a TCR sequencing technology that provides TCR repertoire data with high sensitivity and reliability.
Disclosed herein are methods and kits for sequencing of T-cell receptor repertoires and other immune cell repertoires, such as B-cell repertoires, with high sensitivity and reliability. In one embodiment, the methods include the steps of (1) providing RNA from T-cells, (2) transcribing RNA into complimentary RNA (cRNA), (3) reverse transcribing the cRNA into cDNA while introducing a common adapter to the 5′ end of the cDNA products, (4) amplifying the cDNA using a single primer pair, (5) further amplifying with PCR products with a single primer pair which introduces adapters for next generation sequencing, wherein the first primer binds to the common adapter region, and wherein the second primer binds to the constant region of the TCR gene, and (6) sequencing the PCR products. In one embodiment, the methods include the steps of (1) providing RNA from T-cells, (2) reverse transcribing the RNA into cDNA, (3) generating second strand cDNA while introducing a common adapter to the 5′ end of the cDNA products, (4) amplifying the cDNA using a single primer pair, (5) further amplifying with PCR products with a single primer pair which introduces adapters for next generation sequencing, wherein the first primer binds to the common adapter region, and wherein the second primer binds to the constant region of the TCR gene, and (6) sequencing the PCR products. These embodiments are also called SEQTR method (Sequencing T-cell Receptors). Also provided are kits containing primer mixtures for the sequencing of T-cell receptor repertoires. Similar methods and kits for sequencing of B-cell receptor repertoires are provided.
According to one aspect, methods for sequencing immune cell receptor genes are provided. The methods include (1) providing RNA from immune cells; 2)(a) optionally transcribing the RNA into complementary RNA (cRNA), followed by reverse transcribing the cRNA into complementary DNA (cDNA) using one or more primers that comprise a first adapter sequence, wherein each 5′ end of the cDNA produced by reverse transcription contains the first adapter sequence; (2)(b) if step (2)(a) is not performed, reverse transcribing the RNA into complementary DNA (cDNA), followed by transcribing the cDNA into second strand cDNA using one or more primers that comprise a first adapter sequence, wherein each 5′ end of the cDNA produced by transcribing the cDNA into second strand cDNA contains the first adapter sequence; (3) amplifying the cDNA to produce a first amplification product using a first primer pair comprising a first primer that hybridizes to the first adapter sequence and a second primer that hybridizes to a constant region of immune cell receptor gene; (4) amplifying the first amplification product to produce a second amplification product using a second primer pair, in which (i) a first primer of the second primer pair binds to the adapter sequence at the 5′ end of the second amplification product, (ii) the second primer of the second primer pair binds to the constant region of immune cell receptor gene in the second amplification product, and (iii) the first and second primers comprise adapter sequences for sequencing; and (5) sequencing the second amplification product.
In some embodiments, the reverse transcription step results in PCR products ranging from 150-600 bp. In some embodiments, the immune cell receptor genes are T-cell receptor (TCR) genes or B-cell receptor (BCR) genes.
In some embodiments, the one or more primers used for reverse transcription (step (2)(a)) or second strand cDNA synthesis (step (2)(b)) hybridize to TCR α chain V segments. In some embodiments, the one or more primers used for reverse transcription (step (2)(a)) or second strand cDNA synthesis (step (2)(b)) comprise one or more of SEQ ID NOs: 1-50 or SEQ ID NOs: 261-310.
In some embodiments, the one or more primers used for reverse transcription (step (2)(a)) or second strand cDNA synthesis (step (2)(b)) hybridize to TCR β chain V segments. In some embodiments, the one or more primers used for reverse transcription (step (2)(a)) or second strand cDNA synthesis (step (2)(b)) comprise one or more of SEQ ID NOs: 51-100 or SEQ ID NOs: 311-360.
In some embodiments, the one or more primers used for reverse transcription (step (2)(a)) or second strand cDNA synthesis (step (2)(b)) hybridize to TCR γ chain V segments.
In some embodiments, the one or more primers used for reverse transcription (step (2)(a)) or second strand cDNA synthesis (step (2)(b)) hybridize to TCR δ chain V segments.
In some embodiments, the one or more primers used for reverse transcription (step (2)(a)) or second strand cDNA synthesis (step (2)(b)) hybridize to BCR heavy chain V segments.
In some embodiments, the one or more primers used for reverse transcription (step (2)(a)) or second strand cDNA synthesis (step (2)(b)) hybridize to BCR light chain V segments.
In some embodiments, the one or more primers used for reverse transcription (step (2)(a)) or second strand cDNA synthesis (step (2)(b)) contain a nucleotide barcode sequence. In some embodiments, the nucleotide barcode comprises 6 to 20 nucleotides. In some embodiments, the nucleotide barcode consists of 9 nucleotides. In some embodiments, the nucleotide barcode consists of the sequence NNNNTNNNN, NNNNANNNN or HHHHHNNNN.
In some embodiments, the first adapter sequence of the one or more primers used for the reverse transcription (step (2)(a)) or second strand cDNA synthesis (step (2)(b)) comprises a T7 adapter or an ILLUMINA® adapter.
In some embodiments, the immune cells are T-cells and wherein the second primer of the first pair of primers hybridizes to the constant region of a TCR gene.
In some embodiments, the immune cells are B-cells and wherein the second primer of the first pair of primers hybridizes to the constant region of a BCR gene.
In some embodiments, the sequencing is next generation sequencing.
In some embodiments, the RNA from the immune cells is obtained by mixing immune cells with carrier cells before RNA extraction.
In some embodiments, the immune cells are tumor-infiltrating lymphocytes.
In some embodiments, the immune cells are CD4 or CD8 positive T-cells.
In some embodiments, the immune cells are purified from peripheral blood mononuclear cells (PBMC) before RNA extraction.
In some embodiments, the immune cells are part of a mixture of PBMC.
In some embodiments, the immune cells are derived from a mammal. In some embodiments, the mammal is a human or a mouse.
According to another aspect, kits for sequencing of T-cell receptors are provided. The kits include at least one primer which comprises a TCR α chain V segment portion of any one of SEQ ID NOs: 1-50 or SEQ ID NOs: 261-310 and a barcode sequence. In some embodiments, the kits include at least one primer including any one of SEQ ID NOs: 1-50 or SEQ ID NOs: 261-310.
According to another aspect, kits for sequencing of T-cell receptors are provided. The kits include at least one primer which comprises a TCR β chain V segment portion of any one of SEQ ID NOs: 51-100 or SEQ ID NOs: 311-360 and a barcode sequence. In some embodiments, the kits include at least one primer comprising any one of SEQ ID NOs: 51-100 or SEQ ID NOs: 311-360.
These and other features, aspects, and advantages of the present invention will become better understood with regard to the following description, appended claims and accompanying drawings.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 shows the arrangement of (variable) V, diversity (D), joining (J) and constant (C) regions in the α and β chains of T-cell receptors. Figure taken from Murphy, K., Travers, P., Walport, M., & Janeway, C. (2012). Janeway's immunobiology. New York: Garland Science.
FIG. 2 is an illustration of three different TCR sequencing techniques that have been employed in the past.
FIG. 3 provides an overview of the SEQTR method, using TCR α chains as an example. Each bar represents a TCR α chain gene. In RNA and cRNA molecules, the order of the segments is, left to right: V segments, J segments, and the constant region. Barcode regions are added in cDNA molecule to the left of V segments; and T7 adapter regions are added to the left of the barcodes (also indicated by T7 primer amplification in PCR1 and PCR2 steps). ILLUMINA® sequencing adapters are added in the PCR2 step to the 5′ and 3′ ends of the molecules, as shown in the last set of molecules.
FIG. 4 illustrates the sensitivity of the SEQTR method. 10{circumflex over ( )}6, 10{circumflex over ( )}5, 10{circumflex over ( )}4, 10{circumflex over ( )}3 or 0 CD8 positive T-cells, respectively, were mixed with 5×10{circumflex over ( )}4 3T3 cells. The RNA was extracted and subjected to transcription, reverse transcription and one round of amplification (steps 2-4, see Detailed Description). The resulting PCR products were separated on an agarose gels and visualized with ethidium bromide.
FIG. 5 illustrates the specificity of the SEQTR method. 10{circumflex over ( )}6, 10{circumflex over ( )}5, 10{circumflex over ( )}4, 10{circumflex over ( )}3 or 0 CD8 positive T-cells, respectively, were mixed with 5×10{circumflex over ( )}4 3T3 cells. The RNA was extracted and subjected to the SEQTR method. The percentages of sequencing reads that were or were not, respectively, associated with actual TCR genes are indicated.
FIG. 6 illustrates the unambiguous identification of TCR genes as a feature of the SEQTR method. 5×10{circumflex over ( )}4 3T3 cells were mixed with 10{circumflex over ( )}6, 10{circumflex over ( )}5, 10{circumflex over ( )}4, 10{circumflex over ( )}3 or 0 CD8 positive T-cells, respectively. The RNA of each mixture was isolated and subjected to the SEQTR method. Reads that were not associated with TCR genes were removed from the data set. For the remaining reads, the percentages of reads that could or could not, respectively, be unambiguously assigned to specific V or J segments are indicated.
FIG. 7 illustrates the linearity of the SEQTR method. A fixed amount of DNA encoding a known TCR sequence was diluted at different concentrations into a DNA mixture representing a naïve CD8 T-cell repertoire. TCR repertoires of the individual mixtures were sequenced using the SEQTR method. The observed frequency of the known TCR sequence in the entire repertoire was plotted against the respective TCR gene dilution.
FIG. 8 illustrates the reproducibility of the SEQTR method. TCR repertoires were sequenced using the SEQTR method from one biological sample in two independent technical replicates. The frequencies for each V-J rearrangement/combination in TCR β chains were determined and compared between the two replicates. Each sphere represents a single V-J rearrangement with the size of a sphere indicating the relative frequency of the specific V-J recombination. Grey spheres represent rearrangements for which the relative frequencies detected in the two replicates differed by less than two-fold. Black spheres represent rearrangements for which the relative frequencies detected in the two replicates differed by more than two-fold.
FIGS. 9A-9C illustrates the diversity of three different TCR repertoire sequencing data set using the SEQTR method. FIG. 9A CD8 positive T-cells were isolated from peripheral blood mononuclear cells (PBMC), FIG. 9B additionally purified using tetramers conjugated with neo-epitope TEDYMIHII (SEQ ID NO:236) or FIG. 9C additionally purified using tetramer conjugated with neo-epitope (same as in FIG. 9B) and subsequently expanded in vitro. The TCR repertoires of the respective samples were sequenced using the SEQTR method and the relative frequencies of all observed V/J rearrangements/combinations plotted.
FIGS. 10A-10C illustrate the overlap of TCRs identified using a single cell cloning method and TCRs identified using the SEQTR method. FIG. 10A: T-cells were isolated from PBMC and subjected to an additional round of purification using tetramers conjugated with neo-epitope TEDYMIHII (SEQ ID NO: 236). The resulting cell population was then sorted by fluorescence-activated cell sorting (FACS). Half of the sorted cells were subjected to the SEQTR method to sequence the TCR repertoire. For the other half of cells, individual T-cell clones were isolated and expanded in vitro (single cell cloning). Once the clones were established, the TCR genes of each T-cell clones were amplified and sequenced using classical Sanger sequencing. FIG. 10B: The table shows all six TCRs identified using the single cell cloning method. The sequences correspond to SEQ ID NOs: 237 through 242 from top to bottom, respectively. FIG. 10C: The table shows the eight most frequent TCRs identified using the SEQTR method. The sequences correspond to SEQ ID NOs: 243 through 250 from top to bottom, respectively.
FIG. 11 illustrates the number of reads for different samples obtained using the TCR sequencing service “immunoSEQ®” offered by Adaptive Biotechnology. The requested number of reads per sample was 200,000 reads. The number on the x axis represent analysis of samples from 16 different patients. Columns, left to right, for each sample represent number of reads from: the tumor; the stroma (tissue surrounding the tumor); epitope specific TIL (Tumor Infiltrating Lymphocyte) stained with tetramer and sorted by FACS from the tumor sample (TET); and tetramer sorted TIL from a piece of the tumor that has been engrafted in a mice (mTET).
FIG. 12 illustrates the amplification of TCR genes from T-cells that are part of a PBMC mixture (upper panel) or from isolated, CD4 positive T-cells (lower panel), using steps 2 to 4 of the SEQTR method.
DETAILED DESCRIPTION In light of the shortcomings of existing techniques to sequence TCRs, it was determined that a TCR sequencing technology providing the most reliable TCR repertoire data includes the following features:
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- 1) The amplification of TCR genes is linear and does not employ multiplex PCR, therefore avoiding artificial overrepresentation of certain TCR sequences.
- 2) The method is based on RNA and not DNA, thus only providing data for TCR sequences that have undergone rearrangement and that are actually expressed in T-cells.
- 3) TCR genes are sequenced from the 5′ end, providing high quality sequencing data and therefore maximizing reliable and unambiguous identification of the highly homologous V segments.
- 4) Sequencing data include the highly variable CDR3 region, therefore facilitating unambiguous identification of TCR sequences.
The disclosed methods, systems and kits fulfill all these criteria. These same features are of use in sequencing receptors from other immune cells, such as B-cells.
In some embodiments, the immune cell receptor sequencing methods comprise the following steps:
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- (1) Providing total RNA (RNA) as the starting material;
- (2)(a) Transcribing the RNA into complimentary RNA (cRNA) followed by reverse transcribing the cRNA into cDNA, using primers that introduce a common adapter to the 5′ end of the cDNA products;
- (2)(b) If step (2)(a) is not performed, reverse transcribing the RNA into complementary DNA (cDNA), followed by transcribing the cDNA into second strand cDNA using one or more primers that comprise a first adapter sequence, wherein each 5′ end of the cDNA produced by transcribing the cDNA into second strand cDNA contains the first adapter sequence;
- (3) Amplifying the cDNA products using a single primer pair;
- (4) Amplifying the PCR products of step 4 using a single primer pair, in which:
i. the primers introduce adapters for next generation sequencing, and ii. the first primer binds to the common adapter region at the 5′ end of the PCR products, and iii. the second primer binds to a region of the PCR products that constitutes the constant region of the TCR to be sequenced; and
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- (5) Sequencing the PCR products generated in step 4.
Genetic Information to be Sequenced The genetic information to be sequenced is immune cell receptor genes. In the some embodiments of the invention, the genetic information to be sequenced comprises T-cell receptors genes. In some embodiments, the TCR genes that are sequenced encode TCR α chains or TCR β chains. In other embodiments, TCR genes that are sequenced encode TCR δ chains or TCR γ chains.
In other embodiments of the invention, the genetic information to be sequenced comprises B-cell receptor (BCR) genes.
Starting Material (Step 1) RNA is isolated from immune cells and used to generate complimentary RNA (cRNA) by in vitro transcription. This is in contrast to existing TCR sequencing techniques that use DNA or complementary DNA (cDNA) as their genetic starting material.
In some embodiments, the immune cells from which RNA is obtained are isolated from peripheral blood mononuclear cells before RNA extraction. The immune cells are, in some embodiments, T-cells or B-cells.
In some embodiments, T-cells from which RNA is obtained express CD4 or CD8.
Generation of cRNA Through Transcription (Step (2)(a))
Complementary RNA (cRNA) is generated by in vitro transcription. Any method for performing in vitro transcription known to those skilled in molecular biology can be used. In some embodiments, the in vitro transcription in step 2 is performed using commercially available kits, such as the AMBION™ kits available from Thermo Fisher Scientific.
Reverse Transcription (Step (2)(a))
Reverse transcription of the cRNA is performed to generate complementary DNA (cDNA). Methods known to persons skilled in molecular biology are used to reverse transcribe cRNA to cDNA. Typically, such methods include hybridization of a primer to the 3′ end of the cRNA molecule and production of DNA starting at the hybridized primer using a reverse transcriptase enzyme and appropriate nucleotides, salts and buffers.
The choice of primers used in the reverse transcription reaction is important for the ability to differentiate between homologous, yet distinct, immune cell receptor sequences with high degrees of certainty and allows shortening of the V segments from the 5′ end, generating PCR products with a size of 250-300 bp. Such a size range of PCR products is optimal for next generation sequencing.
In some embodiments, the primers used for the reverse transcription are designed to bind within the V segments of the TCR genes (see FIG. 3). For example, the reverse transcription primers are designed to bind close enough to the V(D)J junction so that the resulting sequencing data cover the CDR3 of the V segment and the J segment, but far enough from the V(D)J junction to still allow differentiation between different V regions.
In some embodiments of the invention, a set of preferred primers is used (see, e.g., the sequences in Table 2 and Table 4, and Table 8 and Table 9). Due to the high degree of homology between different V segments, some of the primers described in Table 2 and Table 4 (and Table 8 and Table 9) bind to more than one V segment (see Table 3 and Table 5; the binding sites in their respective V segments for primers SEQ ID NOs: 1-100 and SEQ ID NOs: 261-360 are indicated in Table 15 and Table 16). However, the design of the primers presented in Table 2 and Table 4 (likewise Table 8 and Table 9) still allows the unambiguous assignment/identification of the respective V segments based on differences between the V segments downstream of the primer-binding site. In an alternative embodiment of the invention, only a subset of the preferred primers SEQ ID NOs: 1-100 and SEQ ID NOs: 261-360 may be used for the reverse transcription.
In yet another embodiment of the invention, primer sets may be used that bind to different regions in the V segments when compared to the primers having SEQ ID NOs: 1-100 and SEQ ID NOs: 261-360. For instance, the binding site of one or more primers may be moved towards the CDR3 region of the TCR gene. Due to the high degree of homology between V segments, the further the primer binding site is moved in the direction of the CDR3 region of the TCR gene, the larger the likelihood that the resulting sequencing data are consistent with the presence of more than one V segment. While, in these cases, the respective V segments cannot be assigned or identified unambiguously, the number of V/J segments possibly present in the sample can often be narrowed down to a small subset. Depending on the application, such limited information can already be of value to the experimenter.
In another embodiment of the invention, the binding site of one or more primers may be moved towards the 5′ end of the V segment as compared to the binding sites of primers SEQ ID NOs: 1-100 and SEQ ID NOs: 261-360. Many next generation sequencing technologies generate sequencing reads that are 150 bp long. Therefore, the further the primer binding site is moved towards the 5′ end of the V segment, the larger is the probability that the respective J segment (which can be found at the 3′ end of the resulting sequencing read) cannot be identified unambiguously. However, this problem can be circumvented by using alternative sequencing technologies that generate reads >150 bp.
In some embodiments, the primers used in step (2)(a) additionally contain a unique bar code. Such barcoding of each RNA molecule before the amplification can be used to correct the obtained sequencing results for PCR and sequencing errors.
In some embodiments, the primers for this reverse transcription step introduce a common T7 adapter at the 5′ end of the resulting PCR products. However, alternative adapter sequences are possible, including, but not limited to ILLUMINA® adapters and sequences presented in Table 1.
TABLE 1
Examples for alternative nucleotide adapters that can
be used instead of a T7 adapter sequence
SEQ ID NO Primer name Primer sequence (5′ to 3′)
251 Original Eberwine T7 AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGCGCT
252 Affymetrix T7 GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGGT
253 Invitrogen T7 TAATACGACTCACTATAGGGAGGCGGT
254 Ambion T7 GGTAATACGACTCACTATAGGGAGAAGAGT
255 Agilent T7 AATTAATACGACTCACTATAGGGAGAT
Reverse Transcription (Step (2)(b))
Reverse transcription of the RNA is performed to generate complementary DNA (cDNA). Methods known to persons skilled in molecular biology are used to reverse transcribe RNA to cDNA. Typically, such methods include hybridization of a primer to the 3′ end of the RNA molecule and production of DNA starting at the hybridized primer using a reverse transcriptase enzyme and appropriate nucleotides, salts and buffers.
Transcribing the cDNA into Second Strand cDNA (Step (2)(b))
Following generation of cDNA, second strand cDNA is synthesized using methods known to persons skilled in molecular biology. Typically, such methods include hybridization of a primer to the 3′ end of the cDNA molecule and production of second strand cDNA starting at the hybridized primer using a polymerase enzyme and appropriate nucleotides, salts and buffers.
The choice of primers used in the second strand synthesis reaction is step (2)(b) is as described above for reverse transcription in step (2)(a). The choice of primers is important for the ability to differentiate between homologous, yet distinct, immune cell receptor sequences with high degrees of certainty and allows shortening of the V segments from the 5′ end, generating PCR products with a size of 250-300 bp. Such a size range of PCR products is optimal for next generation sequencing.
Amplification (Step 3) Amplification of the cDNA is performed by any of the well-known amplification reactions, such as polymerase chain reaction (PCR). Methods known to persons skilled in the molecular biology art are used to amplify the cDNA or a portion thereof (e.g., as depicted in FIG. 3). Typically, such methods include hybridization of a pair of primers to the cDNA molecule and amplification of the DNA sequence between the hybridized primers using a polymerase enzyme and appropriate nucleotides, salts and buffers.
In some embodiments, the first primer of a primer pair used in an amplification step binds to the common adapter region of the cDNA products produced in step 3 and the second primer of the primer pair binds to a region of the cDNA products that constitutes the constant region of the TCR to be sequenced (see FIG. 3).
Of note, not all reverse primers designed to target the constant region of the TCR gene perform equally well in this reaction. For example, the primers listed in Table 7 all failed to provide good amplification with the selected T7 5′ adapter. Therefore, in certain embodiments, the primers listed in are Table 6 used in this amplification step.
Amplification (Step 4) A second amplification step is performed to add additional sequences to the amplified molecules, such as sequences that are useful in downstream DNA sequencing reactions. In some embodiments of the present invention, the primers used in this step add appropriate adapters for ILLUMINA® sequencing.
Sequencing (Step 5) Various suitable sequencing methods described herein or known in the art are used to obtain sequence information from the amplified sequences from the nucleic acid molecules within a sample. For example, sequencing methodologies that can be used in the methods disclosed herein include: classic Sanger sequencing, massively parallel sequencing, next generation sequencing, polony sequencing, 454 pyrosequencing, ILLUMINA® sequencing, SOLEXA® sequencing, SOLID™ sequencing (sequencing by oligonucleotide ligation and detection), ion semiconductor sequencing, DNA nanoball sequencing, heliscope single molecule sequencing, single molecule real time sequencing, nanopore DNA sequencing, tunneling currents DNA sequencing, sequencing by hybridization, sequencing with mass spectrometry, microfluidic Sanger sequencing, microscopy-based sequencing, RNA polymerase sequencing, in vitro virus high-throughput sequencing, Maxam-Gilbert sequencing, single-end sequencing, paired-end sequencing, deep sequencing, and/or ultra-deep sequencing.
Definitions As disclosed herein, a number of ranges of values are provided. It is understood that each intervening value, to the tenth of the unit of the lower limit, unless the context clearly dictates otherwise, between the upper and lower limits of that range is also specifically disclosed. Each smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in that stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range, and each range where either, neither, or both limits are included in the smaller ranges is also encompassed within the invention, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the invention.
As used herein, a “primer” is a nucleic acid molecule that hybridizes with a complementary (including partially complementary) polynucleotide strand. Primers can be DNA molecules, RNA molecules, or DNA or RNA analogs. DNA or RNA analogs can be synthesized from nucleotide analogs.
EXAMPLES Example 1: Exemplary Protocol for the SEQTR Method Using T7 and TrueSeq Adapters TCR α and β chain genes were sequenced in two independent reactions.
1) Starting material and RNA extraction
-
- To obtain sufficient amounts of RNA in the extraction, a minimum of 500,000 T-cells were used as starting material. Alternatively, and especially in instances where fewer T-cells were available, T-cells were mixed with 50,000 mouse 3T3 cells that served as carrier. T-cell RNA was extracted using the RNeasy® Micro Kit from Qiagen Inc. according the manufacturer's instruction with the following modification: Elution was performed with 20 μl of water preheated to 50° C. RNA quality and quantity was verified using a fragment analyzer.
2) cRNA synthesis by in vitro transcription (IVT):
-
- In vitro transcription of isolated RNA was performed using the MessageAmp™ II aRNA Amplification Kit from Ambion® (Thermo Fisher Scientific), which contains enzymes, buffers and nucleotides required to perform the first and second strand cDNA and the in vitro transcription. The kit also provides all columns and reagents needed for the cDNA and cRNA purifications. RNA amplification was performed according to the manufacturer's instructions with the following modifications: 1) Between 0.5 and 1 μg of total RNA as was used as starting material. 2) The IVT was performed in a final volume of 40 μl, and incubated at 37° C. for 16 h. Purified cRNA was quantified by absorbance using a NanoDrop™ spectrophotometer (Thermo Fisher Scientific).
3) cDNA synthesis by reverse transcription:
-
- The reverse transcription of the cRNA was performed with the SuperScript® III from Invitrogen (Thermo Fisher Scientific). The kit provides the enzyme, the buffer and the dithiothreitol (DTT) needed for the reaction. Deoxynucleotides (dNTPs) and RNAsin® Ribonuclease inhibitor were purchased from Promega. The sequences for the primers used for the reverse transcription can be found in Table 2 (primers for sequencing TCR α chain genes) and Table 4 (primers for sequencing TCR β chain genes).
- 500 ng of cRNA were used as starting material for the reverse transcription. cRNA was mixed with 1 μl hTRAV or hTRBV primers mix (2 μM each) and 1 μl dNTP (25 mM) in a final volume of 13 μl. The mix was first incubated at 70° C. for 10 min, then at 50° C. for 30 s. 4 μl 5× buffer, 1 μl DTT (100 mM), 1 μg SuperScript III and 1 μl RNAsin® were added to the mix. The samples were subsequently incubated for at 55° C. 1 h and then at 85° C. for 5 min. After the cDNA synthesis, 1 μg DNase-free RNase (Roche) was added to the cDNA and incubated at 37° C. for 30 min to remove the cRNA.
4) TCR gene amplification:
-
- TCR gene amplification was performed using a Phusion® High-Fidelity DNA polymerase (New England Biolabs) under the following conditions: PCR mix: 1 μl cDNA from step 3, 1 μl dNTPs (25 mM), 1 μl primer mix (10 μM each, see Table 5), 5 μl 5× buffer and 0.2 μl Phusion® enzyme in a total volume of 25 μl.
- PCR conditions:
- 94° C. for 5 min
- 20 to 30 cycles of
- 98° C. for 10 s
- 55° C. for 30 s
- 72° C. for 30 s
- 72° C. for 2 min
- PCR products were purified either from agarose gels (using a Qiaquick Gel Extraction Kit from Qiagen) or using an ExoSAP-IT® PCR Product Cleanup Kit (Affymetrix) according to the manufacturer's instructions.
5) Addition of Next Generation Sequencing adapters:
-
- ILLUMINA® sequencing adapters were added by PCR using a Phusion® High-Fidelity DNA polymerase (New England Biolabs). One third of the purified PCR product obtained in step 4 was mixed with 0.5 μl dNTPs (25 mM), 1 μl primer mix (10 μM each, see Table 8), 5 μl 5× buffer and 0.2 μl Phusion® enzyme in a total volume of 25 μl.
- PCR conditions:
- 94° C. for 5 min
- perform 12 cycles of:
- 98° C. for 10 s
- 55° C. for 30 s
- 72° C. for 30 s
- 72° C. for 2 min
6) TCR library purification:
-
- 10 μl of the PCR product from step 5 were purified using an ExoSAP-IT® PCR Product Cleanup Kit (Affymetrix) or Ampure XP beads (Beckman Coulter) according to the manufacturer's instruction. Samples could then directly be used for ILLUMINA® sequencing.
TABLE 2
Preferred primer sequences for amplification of TCR a chain V segments. N can be any
nucleotide. The sequences for primers presented in this table consist of three parts
(listed from 5′ to 3′): T7 adapter, barcode and TCR a chain V segment.
Sequence
SEQ Sequence T7 adapter barcode portion Sequence TCR a chain V
ID NO Primer name portion of the primer of the primer segment portion of the primer
1 hTRAV1-1 TGTAATACGACTCACTATAG NNNNTNNNN CTTCTACAGGAGCTCCAGATGAAAG
2 hTRAV1-2 TGTAATACGACTCACTATAG NNNNTNNNN CTTTTGAAGGAGCTCCAGATGAAAG
3 hTRAV2 TGTAATACGACTCACTATAG NNNNTNNNN TGCTCATCCTCCAGGTGCGGGA
4 hTRAV3 TGTAATACGACTCACTATAG NNNNTNNNN GAAGAAACCATCTGCCCTTGTGA
5 hTRAV4 TGTAATACGACTCACTATAG NNNNTNNNN CCTGCCCCGGGTTTCCCTGAGCGAC
6 hTRAV5 TGTAATACGACTCACTATAG NNNNTNNNN TCTCTGCGCATTGCAGACACCCA
7 hTRAV6 TGTAATACGACTCACTATAG NNNNTNNNN TTGTTTCATATCACAGCCTCCCA
8 hTRAV7 TGTAATACGACTCACTATAG NNNNTNNNN GCTTGTACATTACAGCCGTGCA
9 hTRAV8-1/8-3 TGTAATACGACTCACTATAG NNNNTNNNN ATCTGAGGAAACCCTCTGTGCA
10 hTRAV8-2/8-4 TGTAATACGACTCACTATAG NNNNTNNNN ACCTGACGAAACCCTCAGCCCAT
11 hTRAV8-5 TGTAATACGACTCACTATAG NNNNTNNNN CCTATGCCTGTCTTTACTTTAATC
12 hTRAV8-6 TGTAATACGACTCACTATAG NNNNTNNNN CTTGAGGAAACCCTCAGTCCATAT
13 hTRAV8-7 TGTAATACGACTCACTATAG NNNNTNNNN GAAACCATCAACCCATGTGAGTGA
14 hTRAV9-1 TGTAATACGACTCACTATAG NNNNTNNNN ACTTGGAGAAAGACTCAGTTCAA
15 hTRAV9-2 TGTAATACGACTCACTATAG NNNNTNNNN ACTTGGAGAAAGGCTCAGTTCAA
16 hTRAV10 TGTAATACGACTCACTATAG NNNNTNNNN CTGCACATCACAGCCTCCCA
17 hTRAV11 TGTAATACGACTCACTATAG NNNNTNNNN GTTTGGAATATCGCAGCCTCTCAT
18 hTRAV12-1 TGTAATACGACTCACTATAG NNNNTNNNN CCCTGCTCATCAGAGACTCCAAG
19 hTRAV12-2 TGTAATACGACTCACTATAG NNNNTNNNN CTCTGCTCATCAGAGACTCCCAG
20 hTRAV12-3 TGTAATACGACTCACTATAG NNNNTNNNN CCTTGTTCATCAGAGACTCACAG
21 hTRAV13-1 TGTAATACGACTCACTATAG NNNNTNNNN TCCCTGCACATCACAGAGACCCAA
22 hTRAV13-2 TGTAATACGACTCACTATAG NNNNTNNNN TCTCTGCAAATTGCAGCTACTCAA
23 hTRAV14 TGTAATACGACTCACTATAG NNNNTNNNN TTGTCATCTCCGCTTCACAACTGG
24 hTRAV15 TGTAATACGACTCACTATAG NNNNTNNNN GTTTTGAATATGCTGGTCTCTCAT
25 hTRAV16 TGTAATACGACTCACTATAG NNNNTNNNN CCTGAAGAAACCATTTGCTCAAGA
26 hTRAV17 TGTAATACGACTCACTATAG NNNNTNNNN TCCTTGTTGATCACGGCTTCCCGG
27 hTRAV18 TGTAATACGACTCACTATAG NNNNTNNNN ACCTGGAGAAGCCCTCGGTGCA
28 hTRAV19 TGTAATACGACTCACTATAG NNNNTNNNN CACCATCACAGCCTCACAAGTCGT
29 hTRAV20 TGTAATACGACTCACTATAG NNNNTNNNN TTTCTGCACATCACAGCCCCTA
30 hTRAV21 TGTAATACGACTCACTATAG NNNNTNNNN CTTTATACATTGCAGCTTCTCAGCC
31 hTRAV22 TGTAATACGACTCACTATAG NNNNTNNNN GTACATTTCCTCTTCCCAGACCAC
32 hTRAV23 TGTAATACGACTCACTATAG NNNNTNNNN CATTGCATATCATGGATTCCCAGC
33 hTRAV24 TGTAATACGACTCACTATAG NNNNTNNNN GCTATTTGTACATCAAAGGATCCC
34 hTRAV25 TGTAATACGACTCACTATAG NNNNTNNNN CAGCTCCCTGCACATCACAGCCA
35 hTRAV26-1 TGTAATACGACTCACTATAG NNNNTNNNN TTGATCCTGCCCCACGCTACGCTGA
36 hTRAV26-2 TGTAATACGACTCACTATAG NNNNTNNNN TTGATCCTGCACCGTGCTACCTTGA
37 hTRAV27 TGTAATACGACTCACTATAG NNNNTNNNN GTTCTCTCCACATCACTGCAGCC
38 hTRAV28 TGTAATACGACTCACTATAG NNNNTNNNN GCCACCTATACATCAGATTCCCA
39 hTRAV29 TGTAATACGACTCACTATAG NNNNTNNNN TCTCTGCACATTGTGCCCTCCCA
40 hTRAV30 TGTAATACGACTCACTATAG NNNNTNNNN CCCTGTACCTTACGGCCTCCCAGCT
41 hTRAV31 TGTAATACGACTCACTATAG NNNNTNNNN CTTATCATATCATCATCACAGCCA
42 hTRAV32 TGTAATACGACTCACTATAG NNNNTNNNN TCCCTGCATATTACAGCCACCCAA
43 hTRAV33 TGTAATACGACTCACTATAG NNNNTNNNN ACCTCACCATCAATTCCTTAAAAC
44 hTRAV34 TGTAATACGACTCACTATAG NNNNTNNNN TCCCTGCATATCACAGCCTCCCAG
45 hTRAV35 TGTAATACGACTCACTATAG NNNNTNNNN CTTCCTGAATATCTCAGCATCCAT
46 hTRAV36 TGTAATACGACTCACTATAG NNNNTNNNN TCCTGAACATCACAGCCACCCAG
47 hTRAV37 TGTAATACGACTCACTATAG NNNNTNNNN TCCCTGCACATACAGGATTCCCAG
48 hTRAV38 TGTAATACGACTCACTATAG NNNNTNNNN CAAGATCTCAGACTCACAGCTGG
49 hTRAV39 TGTAATACGACTCACTATAG NNNNTNNNN CCGTCTCAGCACCCTCCACATCA
50 hTRAV40 TGTAATACGACTCACTATAG NNNNTNNNN CCATTGTGAAATATTCAGTCCAGG
TABLE 3
V segments targeted by each primer used for
the amplification of TCR α chain V segments.
SEQ
ID NO Primer Targeted V segment(s)
1 hTRAV1-1 hTRAV01-1
2 hTRAV1-2 hTRAV01-2
3 hTRAV2 hTRAV02
4 hTRAV3 hTRAV03
5 hTRAV4 hTRAV04
6 hTRAV5 hTRAV05
7 hTRAV6 hTRAV06
8 hTRAV7 hTRAV07
9 hTRAV8-1/8-3 hTRAV08-1, hTRAV08-3
10 hTRAV8-2/8-4 hTRAV08-2, hTRAV08-4
11 hTRAV8-5 hTRAV08-5
12 hTRAV8-6 hTRAV08-6
13 hTRAV8-7 hTRAV08-7
14 hTRAV9-1 hTRAV09-1
15 hTRAV9-2 hTRAV09-2
16 hTRAV10 hTRAV10, hTRAV41
17 hTRAV11 hTRAV11
18 hTRAV12-1 hTRAV12-1
19 hTRAV12-2 hTRAV12-2
20 hTRAV12-3 hTRAV12-3
21 hTRAV13-1 hTRAV13-1
22 hTRAV13-2 hTRAV13-2
23 hTRAV14 hTRAV14
24 hTRAV15 hTRAV15
25 hTRAV16 hTRAV16
26 hTRAV17 hTRAV17
27 hTRAV18 hTRAV18
28 hTRAV19 hTRAV19
29 hTRAV20 hTRAV20
30 hTRAV21 hTRAV21
31 hTRAV22 hTRAV22
32 hTRAV23 hTRAV23
33 hTRAV24 hTRAV24
34 hTRAV25 hTRAV25
35 hTRAV26-1 hTRAV26-1
36 hTRAV26-2 hTRAV26-2
37 hTRAV27 hTRAV27
38 hTRAV28 hTRAV28
39 hTRAV29 hTRAV29
40 hTRAV30 hTRAV30
41 hTRAV31 hTRAV31
42 hTRAV32 hTRAV32
43 hTRAV33 hTRAV33
44 hTRAV34 hTRAV34
45 hTRAV35 hTRAV35
46 hTRAV36 hTRAV36
47 hTRAV37 hTRAV37
48 hTRAV38 hTRAV38-1, hTRAV38-2
49 hTRAV39 hTRAV39
50 hTRAV40 hTRAV40
TABLE 4
Preferred primer sequences for amplification of TCR 3 chain V segments. N can be any
nucleotide. The sequences for primers presented in this table consist of three parts
(listed from 5′ to 3′): T7 adapter, barcode and TCR 3 chain V segment.
Sequence
SEQ Sequence T7 adapter barcode portion Sequence TCR 3 chain V segment
ID NO Primer name portion of the primer of the primer portion of the primer
51 hTRBV1 TGTAATACGACTCACTATAG NNNNANNNN GTGGTCGCACTGCAGCAAGAAGA
52 hTRBV2 TGTAATACGACTCACTATAG NNNNANNNN GATCCGGTCCACAAAGCTGGAGGA
53 hTRBV3-1 TGTAATACGACTCACTATAG NNNNANNNN CATCAATTCCCTGGAGCTTGGTGA
54 hTRBV4-1 TGTAATACGACTCACTATAG NNNNANNNN TTCACCTACACGCCCTGCAGCCAG
55 hTRBV4-2 TGTAATACGACTCACTATAG NNNNANNNN TTCACCTACACACCCTGCAGCCAG
56 hTRBV5-1 TGTAATACGACTCACTATAG NNNNANNNN GAATGTGAGCACCTTGGAGCTGG
57 hTRBV5-2 TGTAATACGACTCACTATAG NNNNANNNN TACTGAGTCAAACACGGAGCTAGG
58 hTRBV5-3 TGTAATACGACTCACTATAG NNNNANNNN GCTCTGAGATGAATGTGAGTGCCT
59 hTRBV5-4 TGTAATACGACTCACTATAG NNNNANNNN CTGAGCTGAATGTGAACGCCTT
60 hTRBV6-1 TGTAATACGACTCACTATAG NNNNANNNN GAGTTCTCGCTCAGGCTGGAGT
61 hTRBV6-2 TGTAATACGACTCACTATAG NNNNANNNN CTGGGGTTGGAGTCGGCTGCTC
62 hTRBV6-4 TGTAATACGACTCACTATAG NNNNANNNN CCCCTCACGTTGGCGTCTGCTG
63 hTRBV6-5 TGTAATACGACTCACTATAG NNNNANNNN TCCCGCTCAGGCTGCTGTCGGC
64 hTRBV6-6 TGTAATACGACTCACTATAG NNNNANNNN GATTTCCCGCTCAGGCTGGAGT
65 hTRBV6-7 TGTAATACGACTCACTATAG NNNNANNNN TCCCCCTCAAGCTGGAGTCAGCT
66 hTRBV6-8 TGTAATACGACTCACTATAG NNNNANNNN TCCCACTCAGGCTGGTGTCGGC
67 hTRBV7-1 TGTAATACGACTCACTATAG NNNNANNNN CTCTGAAGTTCCAGCGCACACA
68 hTRBV7-2 TGTAATACGACTCACTATAG NNNNANNNN GATCCAGCGCACACAGCAGGAG
69 hTRBV7-3 TGTAATACGACTCACTATAG NNNNANNNN ACTCTGAAGATCCAGCGCACAGA
70 hTRBV7-5 TGTAATACGACTCACTATAG NNNNANNNN AGATCCAGCGCACAGAGCAAGG
71 hTRBV7-6 TGTAATACGACTCACTATAG NNNNANNNN CAGCGCACAGAGCAGCGGGACT
72 hTRBV7-9 TGTAATACGACTCACTATAG NNNNANNNN GAGATCCAGCGCACAGAGCAGG
73 hTRBV8-1 TGTAATACGACTCACTATAG NNNNANNNN CCCTCAACCCTGGAGTCTACTA
74 hTRBV8-2 TGTAATACGACTCACTATAG NNNNANNNN TCCCCAATCCTGGCATCCACCA
75 hTRBV9 TGTAATACGACTCACTATAG NNNNANNNN CTAAACCTGAGCTCTCTGGAGCT
76 hTRBV10-1 TGTAATACGACTCACTATAG NNNNANNNN CCCTCACTCTGGAGTCTGCTGC
77 hTRBV10-2 TGTAATACGACTCACTATAG NNNNANNNN CCCTCACTCTGGAGTCAGCTAC
78 hTRBV10-3 TGTAATACGACTCACTATAG NNNNANNNN TCCTCACTCTGGAGTCCGCTAC
79 hTRBV11-1 TGTAATACGACTCACTATAG NNNNANNNN CCACTCTCAAGATCCAGCCTGCA
80 hTRBV12-1 TGTAATACGACTCACTATAG NNNNANNNN GAGGATCCAGCCCATGGAACCCA
81 hTRBV12-2 TGTAATACGACTCACTATAG NNNNANNNN CTGAAGATCCAGCCTGCAGAGC
82 hTRBV12-3 TGTAATACGACTCACTATAG NNNNANNNN CAGCCCTCAGAACCCAGGGACT
83 hTRBV13 TGTAATACGACTCACTATAG NNNNANNNN GAGCTCCTTGGAGCTGGGGGACT
84 hTRBV14 TGTAATACGACTCACTATAG NNNNANNNN GGTGCAGCCTGCAGAACTGGAG
85 hTRBV15 TGTAATACGACTCACTATAG NNNNANNNN GACATCCGCTCACCAGGCCTGG
86 hTRBV16 TGTAATACGACTCACTATAG NNNNANNNN TGAGATCCAGGCTACGAAGCTT
87 hTRBV17 TGTAATACGACTCACTATAG NNNNANNNN GAAGATCCATCCCGCAGAGCCG
88 hTRBV18 TGTAATACGACTCACTATAG NNNNANNNN GGATCCAGCAGGTAGTGCGAGG
89 hTRBV19 TGTAATACGACTCACTATAG NNNNANNNN CACTGTGACATCGGCCCAAAAG
90 hTRBV20 TGTAATACGACTCACTATAG NNNNANNNN CTGACAGTGACCAGTGCCCATC
91 hTRBV21 TGTAATACGACTCACTATAG NNNNANNNN GAGATCCAGTCCACGGAGTCAG
92 hTRBV22 TGTAATACGACTCACTATAG NNNNANNNN GTGAAGTTGGCCCACACCAGCCA
93 hTRBV23 TGTAATACGACTCACTATAG NNNNANNNN CCTGGCAATCCTGTCCTCAGAA
94 hTRBV24 TGTAATACGACTCACTATAG NNNNANNNN GAGTCTGCCATCCCCAACCAGA
95 hTRBV25 TGTAATACGACTCACTATAG NNNNANNNN GGAGTCTGCCAGGCCCTCACA
96 hTRBV26 TGTAATACGACTCACTATAG NNNNANNNN GAAGTCTGCCAGCACCAACCAG
97 hTRBV27 TGTAATACGACTCACTATAG NNNNANNNN GGAGTCGCCCAGCCCCAACCAG
98 hTRBV28 TGTAATACGACTCACTATAG NNNNANNNN GGAGTCCGCCAGCACCAACCAG
99 hTRBV29 TGTAATACGACTCACTATAG NNNNANNNN GTGAGCAACATGAGCCCTGAAGA
100 hTRBV30 TGTAATACGACTCACTATAG NNNNANNNN GAGTTCTAAGAAGCTCCTTCTCA
TABLE 5
V segments targeted by each primer used for
the amplification of TCR β chain V segments.
SEQ TCR b chain V
ID NO segment name Targeted V segment(s)
51 hTRBV1 hTRBV01
52 hTRBV2 hTRBV02
53 hTRBV3-1 hTRBV03-1, hTRBV03-2
54 hTRBV4-1 hTRBV04-1
55 hTRBV4-2 hTRBV04-2, hTRBV04-3
56 hTRBV5-1 hTRBV05-1
57 hTRBV5-2 hTRBV05-2
58 hTRBV5-3 hTRBV05-3
59 hTRBV5-4 hTRBV05-4, hTRBV05-5, hTRBV05-6,
hTRBV05-7, hTRBV05-8
60 hTRBV6-1 hTRBV06-1
61 hTRBV6-2 hTRBV06-2, hTRBV06-3
62 hTRBV6-4 hTRBV06-4
63 hTRBV6-5 hTRBV06-5
64 hTRBV6-6 hTRBV06-6, hTRBV06-9
65 hTRBV6-7 hTRBV06-7
66 hTRBV6-8 hTRBV06-8
67 hTRBV7-1 hTRBV07-1
68 hTRBV7-2 hTRBV07-2, hTRBV07-8
69 hTRBV7-3 hTRBV07-3, hTRBV07-4
70 hTRBV7-5 hTRBV07-5
71 hTRBV7-6 hTRBV07-6, hTRBV07-7
72 hTRBV7-9 hTRBV07-9
73 hTRBV8-1 hTRBV08-1
74 hTRBV8-2 hTRBV08-2
75 hTRBV9 hTRBV09
76 hTRBV10-1 hTRBV10-1
77 hTRBV10-2 hTRBV10-2
78 hTRBV10-3 hTRBV10-3
79 hTRBV11-1 hTRBV11-1, hTRBV11-2, hTRBV11-3
80 hTRBV12-1 hTRBV12-1
81 hTRBV12-2 hTRBV12-2
82 hTRBV12-3 hTRBV12-3, hTRBV12-4, hTRBV12-5
83 hTRBV13 hTRBV13
84 hTRBV14 hTRBV14
85 hTRBV15 hTRBV15
86 hTRBV16 hTRBV16
87 hTRBV17 hTRBV17
88 hTRBV18 hTRBV18
89 hTRBV19 hTRBV19
90 hTRBV20 hTRBV20
91 hTRBV21 hTRBV21
92 hTRBV22 hTRBV22
93 hTRBV23 hTRBV23
94 hTRBV24 hTRBV24
95 hTRBV25 hTRBV25
96 hTRBV26 hTRBV26
97 hTRBV27 hTRBV27
98 hTRBV28 hTRBV28
99 hTRBV29 hTRBV29
100 hTRBV30 hTRBV30
TABLE 6
Primers for TCR gene amplification. Primer pair for sequencing of
TCR α genes: SEQ ID NO 101 and 102. Primer pair for sequencing
of TCR β genes: SEQ ID NO 101 and 103.
SEQ ID NO Primer name Primer sequence TCR chain
101 Forward primer T7 TRAV/TRBV TGTAATACGACTCACTATAG α and β
102 Reverse primer PCR 1 TRAV GGCCACAGCACTGTTGCTCTTGAAG α
103 Reverse primer PCR 1 TRABV CCACTGTGCACCTCCTTCCCATTC β
TABLE 7
Reverse primers for TCR gene amplification that
did not result in successful
amplification of PCR products.
SEQ ID NO Primer sequence TCR chain
104 TCGACCAGCTTGACATCACAGG α
105 CAGATTTGTTGCTCCAGGCCACAG α
106 TCTGTGATATACACATCAGAATC α
107 GAATCAAAATCGGTGAATAGGCAG α
108 GGCAGACAGACTTGTCACTGGATT α
109 TAGGACACCGAGGTAAAGCCAC β
110 CTGGGTGACGGGTTTGGCCCTAT β
111 TTGACAGCGGAAGTGGTTGC β
112 GGCTGCTCAGGCAGTATCTGGAGTC β
113 GCCAGGCACACCAGTGTGGCCTTTT β
TABLE 8
Primers for addition of Next Generation Sequencing adapters. The primer portion
corresponding to the ILLUMINA ® adapters (forward and reverse) is underlined in forward
and reverse primers shown below. Primer pair for sequencing of TCR α genes: SEQ ID NOS:
114 and 115. Primer pair for sequencing of TCR β genes: SEQ ID NOS: 114 and 116.
SEQ TCR
ID NO Primer name Primer sequence chain
114 Forward primer Illumina_T7 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGC α and β
TRAV/TRBV TCTTCCGATCTTGTAATACGACTCACTATAG
115 Reverse primer PCR 2 TRAC CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGAC α
GTGTGCTCTTCCGATCCTCAGCTGGTACACGGCAGGGTCA
116 Reverse primer PCR 2 TRBC CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGAC β
GTGTGCTCTTCCGATCAAACACAGCGACCTCGGGTGGGAAC
Example 2: Exemplary Protocol for the SEQTR Method Using Nextera Adapters TCR α and β chain genes were sequenced in two independent reactions.
1) Starting material and RNA extraction
-
- To obtain sufficient amounts of RNA in the extraction, a minimum of 500,000 T-cells were used as starting material. Alternatively, and especially in instances where fewer T-cells were available, T-cells were mixed with 50,000 mouse 3T3 cells that served as carrier. T-cell RNA was extracted using the RNeasy® Micro Kit from Qiagen Inc. according the manufacturer's instruction with the following modification: Elution was performed with 20 μl of water preheated to 50° C. RNA quality and quantity was verified using a fragment analyzer.
2) cRNA synthesis by in vitro transcription (IVT):
-
- In vitro transcription of isolated RNA was performed using the MessageAmp™ II aRNA Amplification Kit from Ambion® (Thermo Fisher Scientific), which contains enzymes, buffers and nucleotides required to perform the first and second strand cDNA and the in vitro transcription. The kit also provides all columns and reagents needed for the cDNA and cRNA purifications. RNA amplification was performed according to the manufacturer's instructions with the following modifications:
- 1) Between 0.5 and 1 μg of total RNA as was used as starting material. 2) The IVT was performed in a final volume of 40 μl, and incubated at 37° C. for 16 h. Purified cRNA was quantified by absorbance using a NanoDrop™ spectrophotometer (Thermo Fisher Scientific).
3) cDNA synthesis by reverse transcription:
-
- The reverse transcription of the cRNA was performed with the SuperScript® III from Invitrogen (Thermo Fisher Scientific). The kit provides the enzyme, the buffer and the dithiothreitol (DTT) needed for the reaction. Deoxynucleotides (dNTPs) and RNAsin® Ribonuclease inhibitor were purchased from Promega. The sequences for the primers used for the reverse transcription can be found in Table 9 (primers for sequencing TCR α chain genes) and Table 10 (primers for sequencing TCR β chain genes).
- 500 ng of cRNA were used as starting material for the reverse transcription. cRNA was mixed with 1 μl hTRAV or hTRBV primers mix (2 μM each) and 1 μl dNTP (25 mM) in a final volume of 13 μl. The mix was first incubated at 70° C. for 10 min, then at 50° C. for 30 s. 4 μl 5× buffer, 1 μl DTT (100 mM), 1 μl SuperScript III and 1 μl RNAsin® were added to the mix. The samples were subsequently incubated for at 55° C. 1 h and then at 85° C. for 5 min. After the cDNA synthesis, 1 μg DNase-free RNase (Roche) was added to the cDNA and incubated at 37° C. for 30 min to remove the cRNA.
4) TCR gene amplification:
-
- TCR gene amplification was performed using a Phusion® High-Fidelity DNA polymerase (New England Biolabs) under the following conditions:
- PCR mix: 1 μl cDNA from step 3, 0.4 μl dNTPs (10 mM), 0.4 μl primer mix (20 μM Nextera 5′, 10 μM Reverse primer PCR 1 TRAV or 2.5 μM Reverse primer PCR1 TRBV, see Table 11), 2 μl 5× buffer and 0.2 μl Phusion® enzyme in a total volume of 10 μl.
- PCR conditions:
- 94° C. for 5 min
- 20 cycles of
- 98° C. for 10 s
- 55° C. for 30 s
- 72° C. for 30 s
- 72° C. for 2 min
- PCR products were purified using 1 μl of ExoSAP-IT® PCR Product Cleanup Kit (Affymetrix) according to the manufacturer's instructions.
5) Addition of Next Generation Sequencing adapters:
-
- ILLUMINA® sequencing adapters were added by PCR using a Phusion® High-Fidelity DNA polymerase (New England Biolabs). The following mix was added to the 11 μl of PCR1: 1 μl dNTPs (10 mM), 1 μl primer mix (1.25 μM each, see Table 12), 3 μl 5× buffer and 0.2 μl Phusion® enzyme and 9.8 μl of H2O.
- PCR conditions:
- 94° C. for 5 min
- perform 25 cycles of:
- 98° C. for 10 s
- 55° C. for 30 s
- 72° C. for 30 s
- 72° C. for 2 min
6) TCR library purification:
-
- 10 μl of the PCR product from step 5 were purified using an AMPURE XP beads (Beckman Coulter) according to the manufacturer's instruction. Samples could then directly be used for ILLUMINA® sequencing.
TABLE 9
Preferred primer sequences for amplification of TCR α chain V segments. N can be any
nucleotide. The sequences for primers presented in this table consist of three parts (listed
from 5′ to 3′): T7 adapter, barcode and TCR α chain V segment.
Sequence
SEQ Primer name Sequence Nextera adapter barcode portion Sequence TCR α chain V segment
ID NO portion of the primer of the primer portion of the primer
261 hTRAV1-1 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CTTCTACAGGAGCTCCAGATGAAAG
GTATAAGAGACAG
262 hTRAV1-2 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CTTTTGAAGGAGCTCCAGATGAAAG
GTATAAGAGACAG
263 hTRAV2 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TGCTCATCCTCCAGGTGCGGGA
GTATAAGAGACAG
264 hTRAV3 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GAAGAAACCATCTGCCCTTGTGA
GTATAAGAGACAG
265 hTRAV4 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CCTGCCCCGGGTTTCCCTGAGCGAC
GTATAAGAGACAG
266 hTRAV5 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TCTCTGCGCATTGCAGACACCCA
GTATAAGAGACAG
267 hTRAV6 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TTGTTTCATATCACAGCCTCCCA
GTATAAGAGACAG
268 hTRAV7 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GCTTGTACATTACAGCCGTGCA
GTATAAGAGACAG
269 hTRAV8-1/8-3 TCGTCGGCAGCGTCAGATGT HHHHHNNNN ATCTGAGGAAACCCTCTGTGCA
GTATAAGAGACAG
270 hTRAV8-2/8-4 TCGTCGGCAGCGTCAGATGT HHHHHNNNN ACCTGACGAAACCCTCAGCCCAT
GTATAAGAGACAG
271 hTRAV8-5 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CCTATGCCTGTCTTTACTTTAATC
GTATAAGAGACAG
272 hTRAV8-6 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CTTGAGGAAACCCTCAGTCCATAT
GTATAAGAGACAG
273 hTRAV8-7 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GAAACCATCAACCCATGTGAGTGA
GTATAAGAGACAG
274 hTRAV9-1 TCGTCGGCAGCGTCAGATGT HHHHHNNNN ACTTGGAGAAAGACTCAGTTCAA
GTATAAGAGACAG
275 hTRAV9-2 TCGTCGGCAGCGTCAGATGT HHHHHNNNN ACTTGGAGAAAGGCTCAGTTCAA
GTATAAGAGACAG
276 hTRAV10 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CTGCACATCACAGCCTCCCA
GTATAAGAGACAG
277 hTRAV11 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GTTTGGAATATCGCAGCCTCTCAT
GTATAAGAGACAG
278 hTRAV12-1 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CCCTGCTCATCAGAGACTCCAAG
GTATAAGAGACAG
279 hTRAV12-2 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CTCTGCTCATCAGAGACTCCCAG
GTATAAGAGACAG
280 hTRAV12-3 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CCTTGTTCATCAGAGACTCACAG
GTATAAGAGACAG
281 hTRAV13-1 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TCCCTGCACATCACAGAGACCCAA
GTATAAGAGACAG
282 hTRAV13-2 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TCTCTGCAAATTGCAGCTACTCAA
GTATAAGAGACAG
283 hTRAV14 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TTGTCATCTCCGCTTCACAACTGG
GTATAAGAGACAG
284 hTRAV15 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GTTTTGAATATGCTGGTCTCTCAT
GTATAAGAGACAG
285 hTRAV16 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CCTGAAGAAACCATTTGCTCAAGA
GTATAAGAGACAG
286 hTRAV17 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TCCTTGTTGATCACGGCTTCCCGG
GTATAAGAGACAG
287 hTRAV18 TCGTCGGCAGCGTCAGATGT HHHHHNNNN ACCTGGAGAAGCCCTCGGTGCA
GTATAAGAGACAG
288 hTRAV19 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CACCATCACAGCCTCACAAGTCGT
GTATAAGAGACAG
289 hTRAV20 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TTTCTGCACATCACAGCCCCTA
GTATAAGAGACAG
290 hTRAV21 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CTTTATACATTGCAGCTTCTCAGCC
GTATAAGAGACAG
291 hTRAV22 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GTACATTTCCTCTTCCCAGACCAC
GTATAAGAGACAG
292 hTRAV23 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CATTGCATATCATGGATTCCCAGC
GTATAAGAGACAG
293 hTRAV24 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GCTATTTGTACATCAAAGGATCCC
GTATAAGAGACAG
294 hTRAV25 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CAGCTCCCTGCACATCACAGCCA
GTATAAGAGACAG
295 hTRAV26-1 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TTGATCCTGCCCCACGCTACGCTGA
GTATAAGAGACAG
296 hTRAV26-2 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TTGATCCTGCACCGTGCTACCTTGA
GTATAAGAGACAG
297 hTRAV27 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GTTCTCTCCACATCACTGCAGCC
GTATAAGAGACAG
298 hTRAV28 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GCCACCTATACATCAGATTCCCA
GTATAAGAGACAG
299 hTRAV29 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TCTCTGCACATTGTGCCCTCCCA
GTATAAGAGACAG
300 hTRAV30 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CCCTGTACCTTACGGCCTCCCAGCT
GTATAAGAGACAG
301 hTRAV31 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CTTATCATATCATCATCACAGCCA
GTATAAGAGACAG
302 hTRAV32 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TCCCTGCATATTACAGCCACCCAA
GTATAAGAGACAG
303 hTRAV33 TCGTCGGCAGCGTCAGATGT HHHHHNNNN ACCTCACCATCAATTCCTTAAAAC
GTATAAGAGACAG
304 hTRAV34 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TCCCTGCATATCACAGCCTCCCAG
GTATAAGAGACAG
305 hTRAV35 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CTTCCTGAATATCTCAGCATCCAT
GTATAAGAGACAG
306 hTRAV36 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TCCTGAACATCACAGCCACCCAG
GTATAAGAGACAG
307 hTRAV37 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TCCCTGCACATACAGGATTCCCAG
GTATAAGAGACAG
308 hTRAV38 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CAAGATCTCAGACTCACAGCTGG
GTATAAGAGACAG
309 hTRAV39 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CCGTCTCAGCACCCTCCACATCA
GTATAAGAGACAG
310 hTRAV40 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CCATTGTGAAATATTCAGTCCAGG
GTATAAGAGACAG
TABLE 10
Preferred primer sequences for amplification of TCR β chain V segments. N can be
any nucleotide. The sequences for primers presented in this table consist of three parts
(listed from 5′ to 3′): T7 adapter, barcode and TCR β chain V segment.
Sequence
SEQ Sequence T7 adapter barcode portion Sequence TCR β chain V segment
ID NO Primer name portion of the primer of the primer portion of the primer
311 hTRBV1 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GTGGTCGCACTGCAGCAAGAAGA
GTATAAGAGACAG
312 hTRBV2 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GATCCGGTCCACAAAGCTGGAGGA
GTATAAGAGACAG
313 hTRBV3-1 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CATCAATTCCCTGGAGCTTGGTGA
GTATAAGAGACAG
314 hTRBV4-1 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TTCACCTACACGCCCTGCAGCCAG
GTATAAGAGACAG
315 hTRBV4-2 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TTCACCTACACACCCTGCAGCCAG
GTATAAGAGACAG
316 hTRBV5-1 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GAATGTGAGCACCTTGGAGCTGG
GTATAAGAGACAG
317 hTRBV5-2 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TACTGAGTCAAACACGGAGCTAGG
GTATAAGAGACAG
318 hTRBV5-3 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GCTCTGAGATGAATGTGAGTGCCT
GTATAAGAGACAG
319 hTRBV5-4 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CTGAGCTGAATGTGAACGCCTT
GTATAAGAGACAG
320 hTRBV6-1 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GAGTTCTCGCTCAGGCTGGAGT
GTATAAGAGACAG
321 hTRBV6-2 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CTGGGGTTGGAGTCGGCTGCTC
GTATAAGAGACAG
322 hTRBV6-4 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CCCCTCACGTTGGCGTCTGCTG
GTATAAGAGACAG
323 hTRBV6-5 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TCCCGCTCAGGCTGCTGTCGGC
GTATAAGAGACAG
324 hTRBV6-6 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GATTTCCCGCTCAGGCTGGAGT
GTATAAGAGACAG
325 hTRBV6-7 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TCCCCCTCAAGCTGGAGTCAGCT
GTATAAGAGACAG
326 hTRBV6-8 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TCCCACTCAGGCTGGTGTCGGC
GTATAAGAGACAG
327 hTRBV7-1 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CTCTGAAGTTCCAGCGCACACA
GTATAAGAGACAG
328 hTRBV7-2 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GATCCAGCGCACACAGCAGGAG
GTATAAGAGACAG
329 hTRBV7-3 TCGTCGGCAGCGTCAGATGT HHHHHNNNN ACTCTGAAGATCCAGCGCACAGA
GTATAAGAGACAG
330 hTRBV7-5 TCGTCGGCAGCGTCAGATGT HHHHHNNNN AGATCCAGCGCACAGAGCAAGG
GTATAAGAGACAG
331 hTRBV7-6 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CAGCGCACAGAGCAGCGGGACT
GTATAAGAGACAG
332 hTRBV7-9 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GAGATCCAGCGCACAGAGCAGG
GTATAAGAGACAG
333 hTRBV8-1 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CCCTCAACCCTGGAGTCTACTA
GTATAAGAGACAG
334 hTRBV8-2 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TCCCCAATCCTGGCATCCACCA
GTATAAGAGACAG
335 hTRBV9 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CTAAACCTGAGCTCTCTGGAGCT
GTATAAGAGACAG
336 hTRBV10-1 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CCCTCACTCTGGAGTCTGCTGC
GTATAAGAGACAG
337 hTRBV10-2 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CCCTCACTCTGGAGTCAGCTAC
GTATAAGAGACAG
338 hTRBV10-3 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TCCTCACTCTGGAGTCCGCTAC
GTATAAGAGACAG
339 hTRBV11-1 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CCACTCTCAAGATCCAGCCTGCA
GTATAAGAGACAG
340 hTRBV12-1 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GAGGATCCAGCCCATGGAACCCA
GTATAAGAGACAG
341 hTRBV12-2 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CTGAAGATCCAGCCTGCAGAGC
GTATAAGAGACAG
342 hTRBV12-3 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CAGCCCTCAGAACCCAGGGACT
GTATAAGAGACAG
343 hTRBV13 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GAGCTCCTTGGAGCTGGGGGACT
GTATAAGAGACAG
344 hTRBV14 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GGTGCAGCCTGCAGAACTGGAG
GTATAAGAGACAG
345 hTRBV15 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GACATCCGCTCACCAGGCCTGG
GTATAAGAGACAG
346 hTRBV16 TCGTCGGCAGCGTCAGATGT HHHHHNNNN TGAGATCCAGGCTACGAAGCTT
GTATAAGAGACAG
347 hTRBV17 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GAAGATCCATCCCGCAGAGCCG
GTATAAGAGACAG
348 hTRBV18 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GGATCCAGCAGGTAGTGCGAGG
GTATAAGAGACAG
349 hTRBV19 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CACTGTGACATCGGCCCAAAAG
GTATAAGAGACAG
350 hTRBV20 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CTGACAGTGACCAGTGCCCATC
GTATAAGAGACAG
351 hTRBV21 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GAGATCCAGTCCACGGAGTCAG
GTATAAGAGACAG
352 hTRBV22 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GTGAAGTTGGCCCACACCAGCCA
GTATAAGAGACAG
353 hTRBV23 TCGTCGGCAGCGTCAGATGT HHHHHNNNN CCTGGCAATCCTGTCCTCAGAA
GTATAAGAGACAG
354 hTRBV24 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GAGTCTGCCATCCCCAACCAGA
GTATAAGAGACAG
355 hTRBV25 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GGAGTCTGCCAGGCCCTCACA
GTATAAGAGACAG
356 hTRBV26 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GAAGTCTGCCAGCACCAACCAG
GTATAAGAGACAG
357 hTRBV27 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GGAGTCGCCCAGCCCCAACCAG
GTATAAGAGACAG
358 hTRBV28 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GGAGTCCGCCAGCACCAACCAG
GTATAAGAGACAG
359 hTRBV29 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GTGAGCAACATGAGCCCTGAAGA
GTATAAGAGACAG
360 hTRBV30 TCGTCGGCAGCGTCAGATGT HHHHHNNNN GAGTTCTAAGAAGCTCCTTCTCA
GTATAAGAGACAG
TABLE 11
Primers for TCR gene amplification. Primer pair for sequencing of TCR α genes:
SEQ ID NOs: 256 and 257. Primer pair for sequencing of TCR β genes: SEQ ID NOs: 256
and 258. The primer portion corresponding to the ILLUMINA ® adapters (forward and
reverse) is underlined in reverse primers shown below.
SEQ ID NO Primer name Primer sequence TCR chain
256 Forward primer Nextera 5′ TCGTCGGCAGCGTC α and β
257 Reverse primer PCR 1 TRAV GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG α
GAATCAAAATCGGTGAATAGGCAG
258 Reverse primer PCR 1 TRBV GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG β
GCCAGGCACACCAGTGTGGCCTTTT
TABLE 12
Primers used to add the full Nextera sequence to both TCRα and TCRβ.
SEQ ID NO Primer name Primer sequence TCR chain
259 Index Read 1 CAAGCAGAAGACGGCATACGAGAT [i7] GTCTCGTGGGCTC α and β
260 Index Read 2 AATGATACGGCGACCACCGAGATCTACAC [i5] TCGTCGGCAGCG α
Example 3: Exemplary Protocol for the SEQTR Method without In Vitro Transcription TCR α and β chain genes were sequenced in two independent reactions.
1) Starting material and RNA extraction
-
- To obtain sufficient amounts of RNA in the extraction, a minimum of 500,000 T-cells were used as starting material. Alternatively, and especially in instances where fewer T-cells were available, T-cells were mixed with 50,000 mouse 3T3 cells that served as carrier. T-cell RNA was extracted using the RNeasy® Micro Kit from Qiagen Inc. according the manufacturer's instruction with the following modification: Elution was performed with 20 μl of water preheated to 50° C. RNA quality and quantity was verified using a fragment analyzer.
2) cDNA synthesis by reverse transcription:
-
- The reverse transcription of the RNA was performed with the SuperScript® III from Invitrogen (Thermo Fisher Scientific) and oligo d(T). The kit provides the enzyme, the buffer and the dithiothreitol (DTT) needed for the reaction. Deoxynucleotides (dNTPs), oligo d(T) and RNAsin® Ribonuclease inhibitor were purchased from Promega.
- 500 ng of RNA were used as starting material for the reverse transcription. RNA was mixed with 1 μl of oligo d(T) and 1 μl dNTP (25 mM) in a final volume of 13 μl. The mix was first incubated at 70° C. for 10 min, then at 50° C. for 30 s. 4 μl 5 × buffer, 1 μl DTT (100 mM), 1 μl SuperScript III and 1 μl RNAsin® were added to the mix. The samples were subsequently incubated for at 55° C. 1 h and then at 85° C. for 5 min.
3) Second strand cDNA synthesis:
-
- cDNA was then used to synthesize the second strand, performed using the Phusion® High-Fidelity DNA polymerase (New England Biolabs) under the following conditions:
- Mix: 20 μl cDNA from step 2, 4 μl dNTPs (10 mM), 2 μl TRAV primer mix (Table 9), 2 μl TRBV primers mix (Table 10), 20 μl 5× buffer, 1 μl Phusion® enzyme in a total volume of 100 μl.
- Synthesis conditions:
- 98° C. for 5 min
- 40° C. for 30 s
- 72° C. for 5 min
4) cDNA purification:
-
- 100 μl of the cDNA product from step 3 were purified using an AMPURE XP beads (Beckman Coulter) according to the manufacturer's instruction.
5) TCR gene amplification:
-
- TCR gene amplification was performed using a Phusion® High-Fidelity DNA polymerase (New England Biolabs) under the following conditions: PCR mix: 7 μl cDNA from step 4, 0.4 μl dNTPs (10 mM), 0.4 μl primer mix (20 μM Nextera5′, 10 μM Reverse primer PCR 1 TRAV or 2.5 μM Reverse primer PCR1 TRBV, see Table 11), 2 μl 5× buffer and 0.2 μl Phusion® enzyme in a total volume of 10 μl.
- PCR conditions:
- 94° C. for 5 min
- 20 cycles of
- 98° C. for 10 s
- 55° C. for 30 s
- 72° C. for 30 s
- 72° C. for 2 min
- PCR products were purified using 1 μl of ExoSAP-IT® PCR Product Cleanup Kit (Affymetrix) according to the manufacturer's instructions.
6) Addition of Next Generation Sequencing adapters:
-
- ILLUMINA® sequencing adapters were added by PCR using a Phusion® High-Fidelity DNA polymerase (New England Biolabs). The following mix was added to the 11 μl of PCR1: 1 μl dNTPs (10 mM), 1 μl primer mix (1.25 μM each, see Table 12), 3 μl 5× buffer and 0.2 μl Phusion® enzyme and 9.8 μl of H2O.
- PCR conditions:
- 94° C. for 5 min
- perform 25 cycles of:
- 98° C. for 10 s
- 55° C. for 30 s
- 72° C. for 30 s
- 72° C. for 2 min
7) TCR library purification:
-
- 10 μl of the PCR product from step 5 were purified using an AMPURE XP beads (Beckman Coulter) according to the manufacturer's instruction. Samples could then directly be used for ILLUMINA® sequencing.
Example 4: Sensitivity of the TCR Sequencing Method One of the challenges of TCR sequencing are the small amounts of genetic material for each T-cell clone. In many cases, the number of T-cells that can be recovered from a given experiment is too small for researchers to directly extract sufficient amounts of RNA for a subsequent amplification of the TCR genes. In such instances, the T-cells of interest can be mixed with 3T3 mouse cells, which serve as a carrier.
5×10{circumflex over ( )}4 3T3 cells were mixed with 10{circumflex over ( )}6, 10{circumflex over ( )}5, 10{circumflex over ( )}4, 10{circumflex over ( )}3 or 0 CD8 positive T-cells, respectively. The RNA of each mixture was isolated and subjected to steps 2 to 4 of the SEQTR method outlined above (see Detailed Description of the Invention). PCR products were separated on an agarose gel and visualized.
No TCR-specific PCR products were observed in samples that only contained 3T3 cells (see FIG. 4). However, TCR-specific bands were detected in all other samples: Increasing amounts of CD8 positive T-cells in the samples were correlated with increasing amounts of TCR-specific PCR products and decreasing intensity of the unspecific dimer primer band. These data demonstrate that the SEQTR method is sensitive enough to amplify TCR genes from as little as 1,000 T-cells, with no detectable background signal from the 3T3 carrier cells.
Example 5: Specificity of the SEQTR Method Another challenge of TCR sequencing is the lack of specific amplification of TCR genes from complex samples. Competing TCR sequencing technologies such as services offered by Adaptive Biotechnology are characterized by up to 90% unspecific amplification. As a result, only as little as 10% of all sequencing data are informative for TCR repertoire determination, increasing cost and duration of any project aiming to sequence TCR repertoires.
5×10{circumflex over ( )}4 3T3 cells were mixed with 10{circumflex over ( )}6, 10{circumflex over ( )}5, 10{circumflex over ( )}4, 10{circumflex over ( )}3 or 0 CD8 positive T-cells, respectively. TCR repertoires for the individual samples were sequenced using the SEQTR method, and the percentage of reads that corresponded to TCR or non-TCR sequences, respectively, was determined. As shown in FIG. 5, 93-97% of all sequencing reads indeed corresponded to TCR genes, independent of the amount of T-cells used as starting material. In summary, these data show that TCR amplification using the SEQTR method is highly specific even when as little as 1,000 T-cells are used as starting material.
Example 6: Unambiguous Identification of TCR Genes In humans, the TCR locus comprises 54 different V segments for the TCR α chain and 65 different V segments for the TCR β chain. However, many of these V segments are highly homologous. Consequently, one of the big challenges of TCR sequencing is to successfully differentiate between two or more TCR gene segments with high degrees of homology. For instance, depending on the choice of primer used in the amplification of the TCR gene and the length of the generated PCR product, the resulting sequencing data might be compatible with more than one V or J segment (in other words, two or more TCR V or J segments show 100% homology in the sequenced region). In these cases, the TCR gene for a specific read cannot be unambiguously assigned/identified.
5×10{circumflex over ( )}4 3T3 cells were mixed with 10{circumflex over ( )}6, 10{circumflex over ( )}5, 10{circumflex over ( )}4, 10{circumflex over ( )}3 or 0 CD8 positive T-cells, respectively. The RNA of each mixture was isolated and subjected to the TCR sequencing method. Out of all the sequencing reads that were identified as TCR genes, it was assessed if the V or J segments could be identified unambiguously. The data show that between 95% and 97% of all TCR sequencing reads could be assigned to a specific TCR segment, even when using as little as 1,000 T-cells as genetic starting material (see FIG. 6). In summary, the data demonstrate the robustness of the SEQTR method as 90 to 93% of all reads can be used to identify TCR sequences once unspecific sequences and ambiguous TCR sequences have been removed.
Due to the homology between V segments, it can be sometimes difficult to clearly identify the TCR sequence. hTRBV6-2 and hTRBV6-3 cannot be differentiated as they have 100% homology and thus will code for the same TCR. Due to their sequences, hTRBV12-3 and hTRBV12-4 cannot be differentiated with the method disclosed herein. Only paired-end sequencing that will catch the 5′-end of the V segment can discriminate these two sequences. Thus the hTRBV12-3 and hTRBV12-4 were considered as a unique sequence for the analysis of the repertoire.
Example 7: Linearity of TCR Gene Amplification Because non-linear amplification of individual TCR sequences can lead to an incorrect over- or underrepresentation of the affected TCR genes in the final TCR repertoire, linearity of amplification is a critical determinant of the reliability and quality of the TCR sequencing data.
To test linearity of TCR gene amplification in our system, a fixed amount of DNA encoding a known TCR sequence was diluted at different concentrations into a DNA pool representing a naïve CD8 repertoire. Subsequently, the TCR repertoire of each sample was analyzed with SEQTR.
The observed frequency of the known TCR sequence in the entire TCR repertoire was then sequenced for each dilution and compared to the expected frequency. The scatter plot in FIG. 7 shows an excellent correlation (R2=0.99) between the dilution and the frequency of the known TCR sequence in the repertoire observed after sequencing. These data confirm the linearity of the amplification and suggest that results obtained using the SEQTR technique are quantitative.
Example 8: Reproducibility of the SEQTR Method The reproducibility of the method was tested by performing two independent technical replicates starting from the same sample. The frequencies for each V-J rearrangement in the TCR β chains were determined and compared between the two replicates, as illustrated in FIG. 8. Each sphere represents a single V-J rearrangement that was detected in both replicates. Each sphere represents a single V-J rearrangement with the size of a sphere indicating the relative frequency of the specific V-J recombination. Grey spheres represent rearrangements for which the relative frequencies detected in the two replicates differed by less than two-fold. Black spheres represent rearrangements for which the relative frequencies detected in the two replicates differed by more than two-fold. Consistent with common practice in the analysis of gene expression data, differences between replaces of less than 2-fold are not considered significant.
The data show that only 13% of all V-J rearrangements showed a significant frequency difference of more than two-fold between the two technical replicates (see FIG. 8 upper inset). However, as illustrated in FIG. 8, V-J recombinations that were significantly different between the technical replicates were rather poorly expressed, as indicated by the small sizes of the black spheres. Therefore, if the frequencies of the individual V-J rearrangement are taken into consideration, only 0.5% of the sequences showed more than a two-fold difference between the replicates (see FIG. 8 lower inset), demonstrating that the SEQTR method is very reproducible.
Example 9: Sequencing of Example Repertoires Using the SEQTR Method The SEQTR method was tested on three different type of CD8 positive T-cells:
-
- (1) T-cell population 1: CD8 positive T-cells isolated from peripheral blood mononuclear cells (PBMCs).
- (2) T-cell population 2: CD8 positive T-cells as in population 1 were FACS sorted using tetramers. Tetramers are MHC molecules presenting a specific peptide, linked to fluorescent dye. Tetramers bind T-cell expressing a TCR that specifically recognizes the peptide. The fluorescent dye allows sorting of the the desired T-cells by FACS.
- (3) T-cell population 3: CD8 positive T-cells as in population 2 that were subsequently expanded in vitro.
The relative frequencies of each V-J rearrangement were determined using the SEQTR method (see FIG. 9). As expected, the naïve TCR repertoire derived from PBMC (population 1) is highly diverse (see FIG. 9A). Almost all the possible V-J rearrangements are represented in the sample, with no single V-J rearrangement exhibiting a frequency of over 11% The repertoire of the tetramer sorted CD8 positive T-cell subset 2 (see FIG. 9B) is less diverse as compared to the naïve one. Not only are fewer V-J rearrangements present in the repertoire overall. Moreover, two V-J rearrangements are clearly dominant, exhibiting frequencies of over 20%. These V-J rearrangements represent the few T-cells that recognize the epitope TEDYMIHII (SEQ ID NO: 236) conjugated to the tetramer and that were enriched during the tetramer purification step. Finally, the rapid clonal expansion (population 3) of the tetramer-purified T-cells enhances the bias of the TCR repertoire towards the T-cell clones already dominating subset 2. Consequently, part of the low frequency V-J rearrangements are lost and not detected anymore (see FIG. 9C). In summary, these data illustrate that the SEQTR method is well suited to differentiate between TCR repertoires with different degrees of diversities.
Example 10: Comparison of the SEQTR Method with Low-Throughput Single Cell Cloning In order to determine how accurate the TCR repertoire data obtained using the SEQTR method were as compared to the true TCR repertoire present in a given T-cell population, we compared our results to data obtained by single cell sequencing.
Tetramer-specific CD8 were sorted from PBMC by FACS. The recovered cell population was split in two. Half of the cells were subjected to the SEQTR method to sequence the TCR repertoire. For the other half of the cells, individual T-cell clones were isolated and expanded in vitro (single cell cloning). Once the clones were established, the TCR genes of each T-cell clones were amplified and sequenced using classical Sanger sequencing (see FIG. 10A).
Among the 42 individual clones tested using the single cell method, six different TCRs were identified (see FIG. 10B). Using the SEQTR method, 116 different TCR genes were found (the eight most frequently observed V-J rearrangements are shown in FIG. 10C, also see Table 13 and Table 14). Indeed, the five TCRs most frequently observed with the single cell cloning technique also correspond to the five clones most frequently observed when applying the SEQTR method. Overall, all six TCR clones identified with single cell sequencing are represented among the eight TCRs with the highest frequencies observed in the SEQTR method. In summary, these data suggest that the SEQTR method produces a true representation of the actual TCR repertoire of a given T-cell population.
TABLE 13
CDR3 regions of TCR clones identified using
the single cell sequencing method.
SEQ ID NO CDR3 region
237 CASSRHVGGVPEAFFG
238 CASSIGRGSEQYFG
239 CASSDVLSGEAFFG
240 CASQGHKNTEAFFG
241 CASSLGPGGVKTNEKLFFG
242 CASSLGPGGVKTNEKLFFG
TABLE 14
Eight most frequently observed V-J
rearrangements of the 116 different TCR genes
identified using the SEQTR method.
SEQ ID NO CDR3 region
243 CASSDVLSGEAFFG
244 CASQGHKNTEAFFG
245 CASSLGPGGVKTNEKLFFG
246 CASSIGRGSEQYFG
247 CASSRHVGGVPEAFFG
248 CASSASKGQPQHFG
249 CASQGHKNTEAFFG
250 CASSLGPGGVKTNEKLFFG
Example 11: TCR Sequencing Services Offered by Adaptive Biotechnology Provide Sequencing Data that May Reflect Up to 90% Unspecific TCR Amplification Tumor samples from 16 patients were collected and the tumor cells were separated from the surrounding tissue (stroma). In addition, epitope-specific TIL were sorted by FACS from the tumor samples using tetramer staining (TET). Finally, the tumor cells were engrafted into humanized mice. After some time, the tumor was collected and epitope specific TIL were sorted by FACS.
DNA extraction was performed for each sample. DNA was sent to Adaptive Biotechnology for TCR sequencing (immunoSEQ® method, survey protocol 200,000-300,000 reads per sample).
In 80% of the samples, the immunoSEQ® method failed to generate 200,000 reads per samples, suggesting that the immunoSEQ® method fails to generate TCR repertoires with significant reliability.
Example 12: Amplification of TCR Genes from PBMC and CD4 Positive T-Cells RNA was isolated from 10{circumflex over ( )}6 PBMC or 10{circumflex over ( )}6 CD4 positive T-cells, respectively, from three independent samples, The RNA was then subjected to steps 2 to 4 of the SEQTR method outlined above (see Detailed Description of the Invention). PCR products were separated on an agarose gel and visualized (see FIG. 12). Only TCR-specific bands are observed, suggesting that the SEQTR method cannot only be used for CD8 positive T-cells (see Example 7), but also for CD4 positive T-cells and even for T-cells that are part of a complex mixture of other PBMCs.
The foregoing examples and description of the embodiments should be taken as illustrating, rather than as limiting the present invention as defined by the claims. As will be readily appreciated, numerous variations and combinations of the features set forth above can be utilized without departing from the present invention as set forth in the claims. Such variations are not regarded as a departure from the scope of the invention, and all such variations are intended to be included within the scope of the following claims. All references cited herein are incorporated by reference herein in their entireties.
As described and claimed herein, including in the accompanying drawings, reference is made to particular features, including method steps. It is to be understood that the disclosure of the invention in this specification includes all possible combinations of such particular features. For example, where a particular feature is disclosed in the context of a particular aspect or embodiment, or a particular claim, that feature can also be used, to the extent possible, in combination with and/or in the context of other particular aspects and embodiments, and in the disclosed methods, systems and kits generally.
Where reference is made herein to a method comprising two or more defined steps, the defined steps can be carried out in any order or simultaneously (except where the context excludes that possibility), and the method can include one or more other steps which are carried out before any of the defined steps, between two of the defined steps, or after all the defined steps (except where the context excludes that possibility).
TABLE 15
TCR α chain V segments and binding sites for primers presented in Table 2 and
Table 9. The sequence for each V segment presented in this table consists
of three parts (listed from 5′ to 3′): Sequence upstream of primer
binding site, sequence of the primer
binding site, sequence downstream of the primer binding site.
hTRAV
Primer sequence
binding downstream
V site of primer
segment Primer SEQ within binding
name name ID NO hTRAV sequence upstream of primer binding site hTRAV site
hTRA hTRA 117 ATGTGGGGAGCTTTCCTTCTCTATGTTTCCATGAAGATGGGAGGCACT CTTCT ACTCTGC
V01-1 V01-1 GCAGGACAAAGCCTTGAGCAGCCCTCTGAAGTGACAGCTGTGGAAGGA ACAGG CTCTTAC
GCCATTGTCCAGATAAACTGCACGTACCAGACATCTGGGTTTTATGGG AGCTC TTCTGCG
CTGTCCTGGTACCAGCAACATGATGGCGGAGCACCCACATTTCTTTCT CAGAT CTGTGAG
TACAATGCTCTGGATGGTTTGGAGGAGACAGGTCGTTTTTCTTCATTC GAAAG AGA
CTTAGTCGCTCTGATAGTTATGGTTACCTC
hTRA hTRA 118 ATGTGGGGAGTTTTCCTTCTTTATGTTTCCATGAAGATGGGAGGCACT CTTTT ACTCTGC
V01-2 V0 1-2 ACAGGACAAAACATTGACCAGCCCACTGAGATGACAGCTACGGAAGGT GAAGG CTCTTAC
GCCATTGTCCAGATCAACTGCACGTACCAGACATCTGGGTTCAACGGG AGCTC CTCTGTG
CTGTTCTGGTACCAGCAACATGCTGGCGAAGCACCCACATTTCTGTCT CAGAT CTGTGAG
TACAATGTTCTGGATGGTTTGGAGGAGAAAGGTCGTTTTTCTTCATTC GAAAG AGA
CTTAGTCGGTCTAAAGGGTACAGTTACCTC
hTRA hTRA 119 ATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAGGGCTTCTCCTC TGCTC GGCAGAT
V02 V02 AACTCTCTCTGGAAGGTTGCAGAAAGCAAGGACCAAGTGTTTCAGCCT ATCCT GCTGCTG
TCCACAGTGGCATCTTCAGAGGGAGCTGTGGTGGAAATCTTCTGTAAT CCAGG TTTACTA
CACTCTGTGTCCAATGCTTACAACTTCTTCTGGTACCTTCACTTCCCG TGCGG CTGTGCT
GGATGTGCACCAAGACTCCTTGTTAAAGGCTCAAAGCCTTCTCAGCAG GA GTGGAGG
GGACGATACAACATGACCTATGAACGGTTCTCTTCATCGC A
hTRA hTRA 120 ATGGCCTCTGCACCCATCTCGATGCTTGCGATGCTCTTCACATTGAGT GAAGA GCGACTC
V03 V03 GGGCTGAGAGCTCAGTCAGTGGCTCAGCCGGAAGATCAGGTCAACGTT AACCA CGCTTTG
GCTGAAGGGAATCCTCTGACTGTGAAATGCACCTATTCAGTCTCTGGA TCTGC TACTTCT
AACCCTTATCTTTTTTGGTATGTTCAATACCCCAACCGAGGCCTCCAG CCTTG GTGCTGT
TTCCTTCTGAAATACATCACAGGGGATAACCTGGTTAAAGGCAGCTAT TGA GAGAGAC
GGCTTTGAAGCTGAATTTAACAAGAGCCAAACCTCCTTCCACCT A
hTRA hTRA 121 ATGAGGCAAGTGGCGAGAGTGATCGTGTTCCTGACCCTGAGTACTTTG CCTGC ACTGCTG
V04 V04 AGCCTTGCTAAGACCACCCAGCCCATCTCCATGGACTCATATGAAGGA CCCGG TGTACTA
CAAGAAGTGAACATAACCTGTAGCCACAACAACATTGCTACAAATGAT GTTTC CTGCCTC
TATATCACGTGGTACCAACAGTTTCCCAGCCAAGGACCACGATTTATT CCTGA GTGGGTG
ATTCAAGGATACAAGACAAAAGTTACAAACGAAGTGGCCTCCCTGTTT GCGAC ACA
ATCCCTGCCGACAGAAAGTCCAGCACTCTGAG
hTRA hTRA 122 ATGAAGACATTTGCTGGATTTTCGTTCCTGTTTTTGTGGCTGCAGCTG TCTCT GACTGGG
V05 V05 GACTGTATGAGTAGAGGAGAGGATGTGGAGCAGAGTCTTTTCCTGAGT GCGCA GACTCAG
GTCCGAGAGGGAGACAGCTCCGTTATAAACTGCACTTACACAGACAGC TTGCA CTATCTA
TCCTCCACCTACTTATACTGGTATAAGCAAGAACCTGGAGCAGGTCTC GACAC CTTCTGT
CAGTTGCTGACGTATATTTTTTCAAATATGGACATGAAACAAGACCAA CCA GCAGAGA
AGACTCACTGTTCTATTGAATAAAAAGGATAAACATCTG GTA
hTRA hTRA 123 ATGGAGTCATTCCTGGGAGGTGTTTTGCTGATTTTGTGGCTTCAAGTG TTGTT GCCTGCA
V06 V06 GACTGGGTGAAGAGCCAAAAGATAGAACAGAATTCCGAGGCCCTGAAC TCATA GACTCAG
ATTCAGGAGGGTAAAACGGCCACCCTGACCTGCAACTATACAAACTAT TCACA CTACCTA
TCCCCAGCATACTTACAGTGGTACCGACAAGATCCAGGAAGAGGCCCT GCCTC CCTCTGT
GTTTTCTTGCTACTCATACGTGAAAATGAGAAAGAAAAAAGGAAAGAA CCA GCTCTAG
AGACTGAAGGTCACCTTTGATACCACCCTTAAACAGAGT ACA
hTRA hTRA 124 ATGGAGAAGATGCGGAGACCTGTCCTAATTATATTTTGTCTATGTCTT GCTTG GCCTGAA
V07 V07 GGCTGGGCAAATGGAGAAAACCAGGTGGAGCACAGCCCTCATTTTCTG TACAT GATTCAG
GGACCCCAGCAGGGAGACGTTGCCTCCATGAGCTGCACGTACTCTGTC TACAG CCACCTA
AGTCGTTTTAACAATTTGCAGTGGTACAGGCAAAATACAGGGATGGGT CCGTG TTTCTGT
CCCAAACACCTATTATCCATGTATTCAGCTGGATATGAGAAGCAGAAA CA GCTGTAG
GGAAGACTAAATGCTACATTACTGAAGAATGGAAGCA ATG
hTRA hTRA 125 ATGCTCCTGTTGCTCATACCAGTGCTGGGGATGATTTTTGCCCTGAGA ATCTG GTGGAGT
V08-1 V08- GATGCCAGAGCCCAGTCTGTGAGCCAGCATAACCACCACGTAATTCTC AGGAA GACACAG
1/08-3 TCTGAAGCAGCCTCACTGGAGTTGGGATGCAACTATTCCTATGGTGGA ACCCT CTGAGTA
ACTGTTAATCTCTTCTGGTATGTCCAGTACCCTGGTCAACACCTTCAG CTGTG CTTCTGT
CTTCTCCTCAAGTACTTTTCAGGGGATCCACTGGTTAAAGGCATCAAG CA GCCGTGA
GGCTTTGAGGCTGAATTTATAAAGAGTAAATTCTCCTTTA ATGC
hTRA hTRA 126 ATGCTCCTGCTGCTCGTCCCAGTGCTCGAGGTGATTTTTACTCTGGGA ACCTG ATGAGCG
V08-2 V08- GGAACCAGAGCCCAGTCGGTGACCCAGCTTGACAGCCACGTCTCTGTC ACGAA ACGCGGC
2/08-4 TCTGAAGGAACCCCGGTGCTGCTGAGGTGCAACTACTCATCTTCTTAT ACCCT TGAGTAC
TCACCATCTCTCTTCTGGTATGTGCAACACCCCAACAAAGGACTCCAG CAGCC TTCTGTG
CTTCTCCTGAAGTACACATCAGCGGCCACCCTGGTTAAAGGCATCAAC CAT TTGTGAG
GGTTTTGAGGCTGAATTTAAGAAGAGTGAAACCTCCTTCC TGA
hTRA hTRA 127 ATGCTCCTGGAGCTTATCCCACTGCTGGGGATACATTTTGTCCTGAGA ATCTG TTGGAGT
V08-3 V08- ACTGCCAGAGCCCAGTCAGTGACCCAGCCTGACATCCACATCACTGTC AGGAA GATGCTG
1/08-3 TCTGAAGGAGCCTCACTGGAGTTGAGATGTAACTATTCCTATGGGGCA ACCCT CTGAGTA
ACACCTTATCTCTTCTGGTATGTCCAGTCCCCCGGCCAAGGCCTCCAG CTGTG CTTCTGT
CTGCTCCTGAAGTACTTTTCAGGAGACACTCTGGTTCAAGGCATTAAA CA GCTGTGG
GGCTTTGAGGCTGAATTTAAGAGGAGTCAATCTTCCTTCA GTGC
hTRA hTRA 128 ATGCTCCTGCTGCTCGTCCCAGTGCTCGAGGTGATTTTTACCCTGGGA ACCTG ATGAGCG
V08-4 V08- GGAACCAGAGCCCAGTCGGTGACCCAGCTTGGCAGCCACGTCTCTGTC ACGAA ACGCGGC
2/08-4 TCTGAAGGAGCCCTGGTTCTGCTGAGGTGCAACTACTCATCGTCTGTT ACCCT TGAGTAC
CCACCATATCTCTTCTGGTATGTGCAATACCCCAACCAAGGACTCCAG CAGCC TTCTGTG
CTTCTCCTGAAGTACACATCAGCGGCCACCCTGGTTAAAGGCATCAAC CAT CTGTGAG
GGTTTTGAGGCTGAATTTAAGAAGAGTGAAACCTCCTTCC TGACACA
hTRA hTRA 129 ATGCTCCTGGTGCTCATCCCACTGCTGGGGATACATTTTGTCCTGAGT CCTAT TCTTAAT
V08-5 V08-5 GAGAACTGTCAGAGCCCAGTCAGTGACCCAGCCTGACATCCGCATCAC GCCTG CCTGTCA
TGTCTCTGAAGGAGCCTCACTGGAGTTGAGATGTAACTATTCCTATGG TCTTT GCTGAGG
GGCGATGTTGTGGGAAGTCAGGGACCCCAAACGGAGGGACCGGCTGAA ACTTT AGGATGT
GCCATGGCAGAAGAATGTGGATTGTGAAGATTTCATGGACATTTATTA AATC ATGTCAC
GTTCCCCAAATTAATACTTTTATAATTTCTTATGCCTCTCTTTACTGC C
AATCTCTAAACATAAATTGTAAAGATTTCATGGACACTTATCACTTCC
CCAATCAATACCCCTGTGATTT
hTRA hTRA 130 ATGCTCCTGCTGCTCGTCCCAGCGTTCCAGGTGATTTTTACCCTGGGA CTTGA AAGCGAC
V08-6 V08-6 GGAACCAGAGCCCAGTCTGTGACCCAGCTTGACAGCCAAGTCCCTGTC GGAAA ACGGCTG
TTTGAAGAAGCCCCTGTGGAGCTGAGGTGCAACTACTCATCGTCTGTT CCCTC AGTACTT
TCAGTGTATCTCTTCTGGTATGTGCAATACCCCAACCAAGGACTCCAG AGTCC CTGTGCT
CTTCTCCTGAAGTATTTATCAGGATCCACCCTGGTTAAAGGCATCAAC ATAT GTGAGTG
GGTTTTGAGGCTGAATTTAACAAGAGTCAAACTTCCTTCCA A
hTRA hTRA 131 ATGCTCTTAGTGGTCATTCTGCTGCTTGGAATGTTCTTCACACTGAGA GAAAC TGCTGCT
V08-7 V08-7 ACCAGAACCCAGTCGGTGACCCAGCTTGATGGCCACATCACTGTCTCT CATCA GAGTACT
GAAGAAGCCCCTCTGGAACTGAAGTGCAACTATTCCTATAGTGGAGTT ACCCA TCTGTGC
CCTTCTCTCTTCTGGTATGTCCAATACTCTAGCCAAAGCCTCCAGCTT TGTGA TGTGGGT
CTCCTCAAAGACCTAACAGAGGCCACCCAGGTTAAAGGCATCAGAGGT GTGA GACAGG
TTTGAGGCTGAATTTAAGAAGAGCGAAACCTCCTTCTACCTGAG
hTRA hTRA 132 ATGAATTCTTCTCCAGGACCAGCGATTGCACTATTCTTAATGTTTGGG ACTTG GAGTCAG
V09-1 V09-1 GGAATCAATGGAGATTCAGTGGTCCAGACAGAAGGCCAAGTGCTCCCC GAGAA ACTCCGC
TCTGAAGGGGATTCCCTGATTGTGAACTGCTCCTATGAAACCACACAG AGACT TGTGTAC
TACCCTTCCCTTTTTTGGTATGTCCAATATCCTGGAGAAGGTCCACAG CAGTT TTCTGTG
CTCCACCTGAAAGCCATGAAGGCCAATGACAAGGGAAGGAACAAAGGT CAA CTCTGAG
TTTGAAGCCATGTACCGTAAAGAAACCACTTCTTTCC TGA
hTRA hTRA 133 ATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCTTACTGCTTGGA ACTTG GTGTCAG
V09-2 V09-2 AGAACCCGTGGAAATTCAGTGACCCAGATGGAAGGGCCAGTGACTCTC GAGAA ACTCAGC
TCAGAAGAGGCCTTCCTGACTATAAACTGCACGTACACAGCCACAGGA AGGCT GGTGTAC
TACCCTTCCCTTTTCTGGTATGTCCAATATCCTGGAGAAGGTCTACAG CAGTT TTCTGTG
CTCCTCCTGAAAGCCACGAAGGCTGATGACAAGGGAAGCAACAAAGGT CAA CTCTGAG
TTTGAAGCCACATACCGTAAAGAAACCACTTCTTTCC TGA
hTRA hTRA 134 ATGAAAAAGCATCTGACGACCTTCTTGGTGATTTTGTGGCTTTATTTT CTGCA GCTCAGC
V10 V10 TATAGGGGGAATGGCAAAAACCAAGTGGAGCAGAGTCCTCAGTCCCTG CATCA GATTCAG
ATCATCCTGGAGGGAAAGAACTGCACTCTTCAATGCAATTATACAGTG CAGCC CCTCCTA
AGCCCCTTCAGCAACTTAAGGTGGTATAAGCAAGATACTGGGAGAGGT TCCCA CATCTGT
CCTGTTTCCCTGACAATCATGACTTTCAGTGAGAACACAAAGTCGAAC GTGGTGA
GGAAGATATACAGCAACTCTGGATGCAGACACAAAGCAAAGCTCT GCG
hTRA hTRA 135 ACGGAGAAGCCCTTGGGAGTTTCATTCTTGATTTCCTCCTGGCAGCTG GTTTG CTGGGAG
V11 VII TGCTGGGTGAATAGACTACATACACTGGAGCAGAGTCCTTCATTCCTG GAATA ATTCAGC
AATATTCAGGAGGGAATGCATGCCGTTCTTAATTGTACTTATCAGGAG TCGCA CACCTAC
AGAACACTCTTCAATTTCCACTGGTTCCGGCAGGATCCGGGGAGAAGA GCCTC TTCTGTG
CTTGTGTCTTTGACCTTAATTCAATCAAGCCAGAAGGAGCAGGGAGAC TCAT CTTTGC
AAATATTTTAAAGAACTGCTTGGAAAAGAAAAATTTTATAGT
hTRA hTRA 136 ATGATATCCTTGAGAGTTTTACTGGTGATCCTGTGGCTTCAGTTAAGC CCCTG CTCAGTG
V12-1 V12-1 TGGGTTTGGAGCCAACGGAAGGAGGTGGAGCAGGATCCTGGACCCTTC CTCAT ATTCAGC
AATGTTCCAGAGGGAGCCACTGTCGCTTTCAACTGTACTTACAGCAAC CAGAG CACCTAC
AGTGCTTCTCAGTCTTTCTTCTGGTACAGACAGGATTGCAGGAAAGAA ACTCC CTCTGTG
CCTAAGTTGCTGATGTCCGTATACTCCAGTGGTAATGAAGATGGAAGG AAG TGGTGAA
TTTACAGCACAGCTCAATAGAGCCAGCCAGTATATTT CA
hTRA hTRA 137 ATGAAATCCTTGAGAGTTTTACTAGTGATCCTGTGGCTTCAGTTGAGC CTCTG CCCAGTG
V12-2 V12-2 TGGGTTTGGAGCCAACAGAAGGAGGTGGAGCAGAATTCTGGACCCCTC CTCAT ATTCAGC
AGTGTTCCAGAGGGAGCCATTGCCTCTCTCAACTGCACTTACAGTGAC CAGAG CACCTAC
CGAGGTTCCCAGTCCTTCTTCTGGTACAGACAATATTCTGGGAAAAGC ACTCC CTCTGTG
CCTGAGTTGATAATGTTCATATACTCCAATGGTGACAAAGAAGATGGA CAG CCGTGAA
AGGTTTACAGCACAGCTCAATAAAGCCAGCCAGTATGTTT
hTRA hTRA 138 ATGAAATCCTTGAGAGTTTTACTGGTGATCCTGTGGCTTCAGTTAAGC CCTTG CCCAGTG
V12-3 V12-3 TGGGTTTGGAGCCAACAGAAGGAGGTGGAGCAGGATCCTGGACCACTC TTCAT ATTCAGC
AGTGTTCCAGAGGGAGCCATTGTTTCTCTCAACTGCACTTACAGCAAC CAGAG CACCTAC
AGTGCTTTTCAATACTTCATGTGGTACAGACAGTATTCCAGAAAAGGC ACTCA CTCTGTG
CCTGAGTTGCTGATGTACACATACTCCAGTGGTAACAAAGAAGATGGA CAG CAATGAG
AGGTTTACAGCACAGGTCGATAAATCCAGCAAGTATATCT CGCACAG
hTRA hTRA 139 ATGACATCCATTCGAGCTGTATTTATATTCCTGTGGCTGCAGCTGGAC TCCCT CCTGAAG
V13-1 V13-1 TTGGTGAATGGAGAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTC GCACA ACTCGGC
CAGGAGGGAGACAGCGCTGTTATCAAGTGTACTTATTCAGACAGTGCC TCACA TGTCTAC
TCAAACTACTTCCCTTGGTATAAGCAAGAACTTGGAAAAGGACCTCAG GAGAC TTCTGTG
CTTATTATAGACATTCGTTCAAATGTGGGCGAAAAGAAAGACCAACGA CCAA CAGCAAG
ATTGCTGTTACATTGAACAAGACAGCCAAACATTTC TA
hTRA hTRA 140 ATGGCAGGCATTCGAGCTTTATTTATGTACTTGTGGCTGCAGCTGGAC TCTCT CCTGGAG
V13-2 V13-2 TGGGTGAGCAGAGGAGAGAGTGTGGGGCTGCATCTTCCTACCCTGAGT GCAAA ACTCAGC
GTCCAGGAGGGTGACAACTCTATTATCAACTGTGCTTATTCAAACAGC TTGCA TGTCTAC
GCCTCAGACTACTTCATTTGGTACAAGCAAGAATCTGGAAAAGGTCCT GCTAC TTTTGTG
CAATTCATTATAGACATTCGTTCAAATATGGACAAAAGGCAAGGCCAA TCAA CAGAGAA
AGAGTCACCGTTTTATTGAATAAGACAGTGAAACATCTC TA
hTRA hTRA 141 ATGTCACTTTCTAGCCTGCTGAAGGTGGTCACAGCTTCACTGTGGCTA TTGTC GGGACTC
V14 V14 GGACCTGGCATTGCCCAGAAGATAACTCAAACCCAACCAGGAATGTTC ATCTC AGCAATG
GTGCAGGAAAAGGAGGCTGTGACTCTGGACTGCACATATGACACCAGT CGCTT TATTTCT
GATCAAAGTTATGGTCTATTCTGGTACAAGCAGCCCAGCAGTGGGGAA CACAA GTGCAAT
ATGATTTTTCTTATTTATCAGGGGTCTTATGACGAGCAAAATGCAACA CTGG GAGAGAG
GAAGGTCGCTACTCATTGAATTTCCAGAAGGCAAGAAAATCCGCCAAC GG
C
hTRA hTRA 142 ATGTATACGTATGTAACAAACCTGCGCGTTGTGCACATGTACCCTAGA GTTTT CCTGGAG
V15 V15 ACGGGTGAACAGCCTCCATATTCTGGAGTAGAGTCCTTCATTCATTCC GAATA ATTCAGG
TGAGTATCCGGGAGGGAATGCACAACATTCTTAATTGCACTTATGAGG TGCTG CACCTAC
AGAGAACGTTCTCTTAACTTCTACTGGTTCTGGCAGGGTCTGGAAAAG GTCTC TTCTGTG
GACTTGTGTCTTTGACCTTAATTCAATCAAGCCAGATGGAGGAGGGAG TCAT CTTTGAG
ACAAACATTTTAAAGAAGCGCTTGGAAAAGAGAAGTTTTATAGT G
hTRA hTRA 143 ATGAAGCCCACCCTCATCTCAGTGCTTGTGATAATATTTATACTCAGA CCTGA GGAAGAC
V16 V16 GGAACAAGAGCCCAGAGAGTGACTCAGCCCGAGAAGCTCCTCTCTGTC AGAAA TCAGCCA
TTTAAAGGGGCCCCAGTGGAGCTGAAGTGCAACTATTCCTATTCTGGG CCATT TGTATTA
AGTCCTGAACTCTTCTGGTATGTCCAGTACTCCAGACAACGCCTCCAG TGCTC CTGTGCT
TTACTCTTGAGACACATCTCTAGAGAGAGCATCAAAGGCTTCACTGCT AAGA CTAAGTG
GACCTTAACAAAGGCGAGACATCTTTCCA G
hTRA hTRA 144 ATGGAAACTCTCCTGGGAGTGTCTTTGGTGATTCTATGGCTTCAACTG TCCTT GCAGCAG
V17 V17 GCTAGGGTGAACAGTCAACAGGGAGAAGAGGATCCTCAGGCCTTGAGC GTTGA ACACTGC
ATCCAGGAGGGTGAAAATGCCACCATGAACTGCAGTTACAAAACTAGT TCACG TTCTTAC
ATAAACAATTTACAGTGGTATAGACAAAATTCAGGTAGAGGCCTTGTC GCTTC TTCTGTG
CACCTAATTTTAATACGTTCAAATGAAAGAGAGAAACACAGTGGAAGA CCGG CTACGGA
TTAAGAGTCACGCTTGACACTTCCAAGAAAAGCAGT CG
hTRA hTRA 145 ATGCTGTCTGCTTCCTGCTCAGGACTTGTGATCTTGTTGATATTCAGA ACCTG GCTGTCG
V18 V18 AGGACCAGTGGAGACTCGGTTACCCAGACAGAAGGCCCAGTTACCCTC GAGAA GACTCTG
CCTGAGAGGGCAGCTCTGACATTAAACTGCACTTATCAGTCCAGCTAT GCCCT CCGTGTA
TCAACTTTTCTATTCTGGTATGTCCAGTATCTAAACAAAGAGCCTGAG CGGTG CTACTGC
CTCCTCCTGAAAAGTTCAGAAAACCAGGAGACGGACAGCAGAGGTTTT CA GCTCTGA
CAGGCCAGTCCTATCAAGAGTGACAGTTCCTTCC GA
hTRA hTRA 146 ATGCTGACTGCCAGCCTGTTGAGGGCAGTCATAGCCTCCATCTGTGTT CACCA GGACTCA
V19 V19 GTATCCAGCATGGCTCAGAAGGTAACTCAAGCGCAGACTGAAATTTCT TCACA GCAGTAT
GTGGTGGAGAAGGAGGATGTGACCTTGGACTGTGTGTATGAAACCCGT GCCTC ACTTCTG
GATACTACTTATTACTTATTCTGGTACAAGCAACCACCAAGTGGAGAA ACAAG TGCTCTG
TTGGTTTTCCTTATTCGTCGGAACTCTTTTGATGAGCAAAATGAAATA TCGT AGTGAGG
AGTGGTCGGTATTCTTGGAACTTCCAGAAATCCACCAGTTCCTTCAAC C
TT
hTRA hTRA 147 ATGGAGAAAATGTTGGAGTGTGCATTCATAGTCTTGTGGCTTCAGCTT TTTCT AACCTGA
V20 V20 GGCTGGTTGAGTGGAGAAGACCAGGTGACGCAGAGTCCCGAGGCCCTG GCACA AGACTCA
AGACTCCAGGAGGGAGAGAGTAGCAGTCTTAACTGCAGTTACACAGTC TCACA GCCACTT
AGCGGTTTAAGAGGGCTGTTCTGGTATAGGCAAGATCCTGGGAAAGGC GCCCC ATCTCTG
CCTGAATTCCTCTTCACCCTGTATTCAGCTGGGGAAGAAAAGGAGAAA TA TGCTGTG
GAAAGGCTAAAAGCCACATTAACAAAGAAGGAAAGC CAGG
hTRA hTRA 148 ATGGAGACCCTCTTGGGCCTGCTTATCCTTTGGCTGCAGCTGCAATGG CTTTA TGGTGAC
V21 V21 GTGAGCAGCAAACAGGAGGTGACGCAGATTCCTGCAGCTCTGAGTGTC TACAT TCAGCCA
CCAGAAGGAGAAAACTTGGTTCTCAACTGCAGTTTCACTGATAGCGCT TGCAG CCTACCT
ATTTACAACCTCCAGTGGTTTAGGCAGGACCCTGGGAAAGGTCTCACA CTTCT CTGTGCT
TCTCTGTTGCTTATTCAGTCAAGTCAGAGAGAGCAAACAAGTGGAAGA CAGCC GTGAGG
CTTAATGCCTCGCTGGATAAATCATCAGGACGTAGTA
hTRA hTRA 149 ATGAAGAGGATATTGGGAGCTCTGCTGGGGCTCTTGAGTGCCCAGGTT GTACA AGACTCA
V22 V22 TGCTGTGTGAGAGGAATACAAGTGGAGCAGAGTCCTCCAGACCTGATT TTTCC GGCGTTT
CTCCAGGAGGGAGCCAATTCCACGCTGCGGTGCAATTTTTCTGACTCT TCTTC ATTTCTG
GTGAACAATTTGCAGTGGTTTCATCAAAACCCTTGGGGACAGCTCATC
AACCTGTTTTACATTCCCTCAGGGACAAAACAGAATGGAAGATTAAGC CCAGA TGCTGTG
GCCACGACTGTCGCTACGGAACGCTACAGCTTATT CCAC GAGC
hTRA hTRA 150 ATGGACAAGATCTTAGGAGCATCATTTTTAGTTCTGTGGCTTCAACTA CATTG CTGGAGA
V23 V23 TGCTGGGTGAGTGGCCAACAGAAGGAGAAAAGTGACCAGCAGCAGGTG CATAT CTCAGCC
AAACAAAGTCCTCAATCTTTGATAGTCCAGAAAGGAGGGATTTCAATT CATGG ACCTACT
ATAAACTGTGCTTATGAGAACACTGCGTTTGACTACTTTCCATGGTAC ATTCC TCTGTGC
CAACAATTCCCTGGGAAAGGCCCTGCATTATTGATAGCCATACGTCCA CAGC AGCAAGC
GATGTGAGTGAAAAGAAAGAAGGAAGATTCACAATCTCCTTCAATAAA A
AGTGCCAAGCAGTTCT
hTRA hTRA 151 ATGGAGAAGAATCCTTTGGCAGCCCCATTACTAATCCTCTGGTTTCAT GCTAT AGCCTGA
V24 V24 CTTGACTGCGTGAGCAGCATACTGAACGTGGAACAAAGTCCTCAGTCA TTGTA AGACTCA
CTGCATGTTCAGGAGGGAGACAGCACCAATTTCACCTGCAGCTTCCCT CATCA GCCACAT
TCCAGCAATTTTTATGCCTTACACTGGTACAGATGGGAAACTGCAAAA AAGGA ACCTCTG
AGCCCCGAGGCCTTGTTTGTAATGACTTTAAATGGGGATGAAAAGAAG TCCC TGCCTTT
AAAGGACGAATAAGTGCCACTCTTAATACCAAGGAGGGTTACA A
hTRA hTRA 152 ATGCTACTCATCACATCAATGTTGGTCTTATGGATGCAATTGTCACAG CAGCT CCCAGAC
V25 V25 GTGAATGGACAACAGGTAATGCAAATTCCTCAGTACCAGCATGTACAA CCCTG TACAGAT
GAAGGAGAGGACTTCACCACGTACTGCAATTCCTCAACTACTTTAAGC CACAT GTAGGAA
AATATACAGTGGTATAAGCAAAGGCCTGGTGGACATCCCGTTTTTTTG CACAG CCTACTT
ATACAGTTAGTGAAGAGTGGAGAAGTGAAGAAGCAGAAAAGACTGACA CCA CTGTGCA
TTTCAGTTTGGAGAAGCAAAAAAGAA GGG
hTRA hTRA 153 ATGAGGCTGGTGGCAAGAGTAACTGTGTTTCTGACCTTTGGAACTATA TTGAT GAGACAC
V26-1 V26-1 ATTGATGCTAAGACCACCCAGCCCCCCTCCATGGATTGCGCTGAAGGA CCTGC TGCTGTG
AGAGCTGCAAACCTGCCTTGTAATCACTCTACCATCAGTGGAAATGAG CCCAC TACTATT
TATGTGTATTGGTATCGACAGATTCACTCCCAGGGGCCACAGTATATC GCTAC GCATCGT
ATTCATGGTCTAAAAAACAATGAAACCAATGAAATGGCCTCTCTGATC GCTGA CAGAGTC
ATCACAGAAGACAGAAAGTCCAGCACC G
hTRA hTRA 154 ATGAAGTTGGTGACAAGCATTACTGTACTCCTATCTTTGGGTATTATG TTGAT GAGATGC
V26-2 V26-2 GGTGATGCTAAGACCACACAGCCAAATTCAATGGAGAGTAACGAAGAA CCTGC TGCTGTG
GAGCCTGTTCACTTGCCTTGTAACCACTCCACAATCAGTGGAACTGAT ACCGT TACTACT
TACATACATTGGTATCGACAGCTTCCCTCCCAGGGTCCAGAGTACGTG GCTAC GCATCCT
ATTCATGGTCTTACAAGCAATGTGAACAACAGAATGGCCTCTCTGGCA CTTGA GAGAGAC
ATCGCTGAAGACAGAAAGTCCAGTACC
hTRA hTRA 155 ATGGTCCTGAAATTCTCCGTGTCCATTCTTTGGATTCAGTTGGCATGG GTTCT CAGCCTG
V27 V27 GTGAGCACCCAGCTGCTGGAGCAGAGCCCTCAGTTTCTAAGCATCCAA CTCCA GTGATAC
GAGGGAGAAAATCTCACTGTGTACTGCAACTCCTCAAGTGTTTTTTCC CATCA AGGCCTC
AGCTTACAATGGTACAGACAGGAGCCTGGGGAAGGTCCTGTCCTCCTG CTGCA TACCTCT
GTGACAGTAGTTACGGGTGGAGAAGTGAAGAAGCTGAAGAGACTAACC GCC GTGCAGG
TTTCAGTTTGGTGATGCAAGAAAGGACA AG
hTRA hTRA 156 ATGAAGGCATTAATAGGAATCTTGCTGGGCTTCCTGTGGATACAGATT GCCAC GCCTGAG
V28 V28 TGCTCGCAAATGAAAGTGGAGCAGAGTCCTCAGGTCCTGATCCTCCAA CTATA GACTCAG
GAGGGAAGAAATTCATTCCTGGTGTGCAGTTGTTCTATTTACATGATC CATCA CTATTTA
CGTGTGCAGTGGTTTCATCAAAAGCCTGGAGGACCCCTCATGTCCTTA GATTC CTTCTGT
TTTAACATTAATTCAGGAATACAGCAAAAAAGAAGACTAAAATCCGCA CCA GCTGTGG
GTCAAAGCTGAGGAACTTTATG GGA
hTRA hTRA 157 ATGGCCATGCTCCTGGGGGCATCAGTGCTGATTCTGTGGCTTCAGCCA TCTCT GCCTGGA
V29 V29 GACTGGGTAAACAGTCAACAGAAGAATGATGACCAGCAAGTTAAGCAA GCACA GACTCTG
AATTCACCATCCCTGAGCGTCCAGGAAGGAAGAATTTCTATTCTGAAC TTGTG CAGTGTA
TGTGACTATACTAACAGCATGTTTGATTATTTCCTATGGTACAAAAAA CCCTC CTTCTGT
TACCCTGCTGAAGGTCCTACATTCCTGATATCTATAAGTTCCATTAAG CCA GCAGCAA
GATAAAAATGAAGATGGAAGATTCACTGTCTTCTTAAACAAAAGTGCC GCG
AAGCACCTC
hTRA hTRA 158 ATGGAGACTCTCCTGAAAGTGCTTTCAGGCACCTTGTTGTGGCAGTTG CCCTG CAGTTAC
V30 V30 ACCTGGGTGAGAAGCCAACAACCAGTGCAGAGTCCTCAAGCCGTGATC TACCT TCAGGAA
CTCCGAGAAGGGGAAGATGCTGTCATCAACTGCAGTTCCTCCAAGGCT TACGG CCTACTT
TTATATTCTGTACACTGGTACAGGCAGAAGCATGGTGAAGCACCCGTC CCTCC CTGCGGC
TTCCTGATGATATTACTGAAGGGTGGAGAACAGAAGGGTCATGAAAAA CAGCT ACAGAGA
ATATCTGCTTCATTTAATGAAAAAAAGCAGCAAAGCT
hTRA hTRA 159 ATGACTGTTGGCAGCATATTACGGGCACTCATGGCCTCTGCCTTCCTT CTTAT GAAGACC
V31 V31 GCATGTCACAGAGGGTCATTCAATCCCAACCAGCAATATCTACGCAGG CATAT TGCAACA
AGGGTGAGACCGTGAAACTGGACTGTGCATACAAAACTAATATTGTAT CATCA TATTTCT
ATTACATATTGTATTGGTACAAAAGGTCTCCCAATGGGAAGATTATTT TCACA GTTGTCT
TCCTCATTTATCAGCAAACAGATGCAGAAACCAATGCGACACAGGGTC GCCA CAAAGAG
AATATTCTGTGAGCTTCCAGAAAACAACTAAAACTATTCAG CC
hTRA hTRA 160 ATGGCAAGAAGAATGGAAAAGTCCCTGGGAGCTTTATTCAAATTCAGC TCCCT CCAGGAG
V32 V32 TGAAGCTGGCCAAGAAAAGGATGTGATACAGAGTTATTCAAATCTAAA GCATA ACTCATT
TGTCTAGGAGAGAGAAATGGCCGTTATTAATGACAGTTATACAGATGG TTACA CCTGTAC
AGCTTTGAATTATTTCTGTTGGTACAAGAAGAAAACGGGGAAGGCCCT GCCAC TTCTGTG
AATATCTTAATGGAGATTCATTCAAATGTGGATAGAAAACAGGACAGA CCAA CAGTGAG
AGGCTCACTGTACTGTTGAATAAAAATGCTAAACATGTC AACACA
hTRA hTRA 161 ATGCTCTGCCCTGGCCTGCTGTGGGCATTCGTGGTCCCCTTTGGCTTC ACCTC TGACTCA
V33 V33 AGATCCAGCATGGCTCAGAAAGTAACCCAAGTTCAGACCACAGTAACT ACCAT GCCAAGT
AGGCAGAAAGGAGTAGCTGTGACCTTGGACTGCATGTTTGAAACCAGA CAATT ACTTCTG
TAGAATTCGTACACTTTATACTGGTACAAGCAACAAGCAACCTCCCAG CCTTA TGCTCTC
TGAAGAGATGGTTTTCCTTATTCATCAGGGTTATTCTAAGTCAAATGC AAAC AGGAATC
AAAGCCTGTGAACTTTGAAAAAAAGAAAAAGTTCATCA C
hTRA hTRA 162 ATGGAGACTGTTCTGCAAGTACTCCTAGGGATATTGGGGTTCCAAGCA TCCCT CCCAGCC
V34 V34 GCCTGGGTCAGTAGCCAAGAACTGGAGCAGAGTCCTCAGTCCTTGATC GCATA ATGCAGG
GTCCAAGAGGGAAAGAATCTCACCATAAACTGCACGTCATCAAAGACG TCACA CATCTAC
TTATATGGCTTATACTGGTATAAGCAAAAGTATGGTGAAGGTCTTATC GCCTC CTCTGTG
TTCTTGATGATGCTACAGAAAGGTGGGGAAGAGAAAAGTCATGAAAAG CCAG GAGCAGA
ATAACTGCCAAGTTGGATGAGAAAAAGCAGCAAAGT CA
hTRA hTRA 163 ATGCTCCTTGAACATTTATTAATAATCTTGTGGATGCAGCTGACATGG CTTCC ACCTAGT
V35 V35 GTCAGTGGTCAACAGCTGAATCAGAGTCCTCAATCTATGTTTATCCAG TGAAT GATGTAG
GAAGGAGAAGATGTCTCCATGAACTGCACTTCTTCAAGCATATTTAAC ATCTC GCATCTA
ACCTGGCTATGGTACAAGCAGGAACCTGGGGAAGGTCCTGTCCTCTTG AGCAT CTTCTGT
ATAGCCTTATATAAGGCTGGTGAATTGACCTCAAATGGAAGACTGACT CCAT GCTGGGC
GCTCAGTTTGGTATAACCAGAAAGGACAG AG
hTRA hTRA 164 ATGATGAAGTGTCCACAGGCTTTACTAGCTATCTTTTGGCTTCTACTG TCCTG ACCGGAG
V36 V36 AGCTGGGTGAGCAGTGAAGACAAGGTGGTACAAAGCCCTCTATCTCTG AACAT ACTCGGC
GTTGTCCACGAGGGAGACACCGTAACTCTCAATTGCAGTTATGAAGTG CACAG CATCTAC
ACTAACTTTCGAAGCCTACTATGGTACAAGCAGGAAAAGAAAGCTCCC CCACC CTCTGTG
ACATTTCTATTTATGCTAACTTCAAGTGGAATTGAAAAGAAGTCAGGA CAG CTGTGGA
AGACTAAGTAGCATATTAGATAAGAAAGAACTTTCCAGCA GG
hTRA hTRA 165 ATGGAAACTCCACTGAGCACTCTGCTGCTGCTCCTCTGTGTGCAGCTG TCCCT CTCCATG
V37 V37 ACCTGGTCAAATGGACAACTGCCAGTGGAACAGAATGCTCCTTCCCTG GCACA ACTCAAC
AAAGTCAAGGAAGGTGACAGCGTCACACTGAACTGCAGTTACAGAGAC TACAG CACATTC
AGCCCTTCAGATTTCTTCAGTGGTTCAGGCAGGATCCTGAGGAAGGCC GATTC TTCTGCG
TCATTTCCCTGATACAAATGCTATCAACTGTGAGAGAGAAGATCAGTG CCAG CAGCAAG
GAAGATTCACAGCCAGGCTTAAAAAAGGAGACCAGCACATT CA
hTRA hTRA 166 ATGACACGAGTTAGCTTGCTGTGGGCAGTCGTGGTCTCCACCTGTCTT CAAGA GGGACAC
V38-1 V38 GAATCCGGCATGGCCCAGACAGTCACTCAGTCTCAACCAGAGATGTCT TCTCA TGCGATG
GTGCAGGAGGCAGAGACTGTGACCCTGAGTTGCACATATGACACCAGT GACTC TATTTCT
GAGAATAATTATTATTTGTTCTGGTACAAGCAGCCTCCCAGCAGGCAG ACAGC GTGCTTT
ATGATTCTCGTTATTCGCCAAGAAGCTTATAAGCAACAGAATGCAACG TGG CATGAAG
GAGAATCGTTTCTCTGTGAACTTCCAGAAAGCAGCCAAATCCTTCAGT CA
CT
hTRA hTRA 167 ATGGCATGCCCTGGCTTCCTGTGGGCACTTGTGATCTCCACCTGTCTT CAAGA GGGATGC
V38-2 V38 GAATTTAGCATGGCTCAGACAGTCACTCAGTCTCAACCAGAGATGTCT TCTCA CGCGATG
GTGCAGGAGGCAGAGACCGTGACCCTGAGCTGCACATATGACACCAGT GACTC TATTTCT
GAGAGTGATTATTATTTATTCTGGTACAAGCAGCCTCCCAGCAGGCAG ACAGC GTGCTTA
ATGATTCTCGTTATTCGCCAAGAAGCTTATAAGCAACAGAATGCAACA TGG TAGGAGC
GAGAATCGTTTCTCTGTGAACTTCCAGAAAGCAGCCAAATCCTTCAGT G
CT
hTRA hTRA 168 ATGAAGAAGCTACTAGCAATGATTCTGTGGCTTCAACTAGACCGGTTA CCGTC CAGCTGC
V39 V39 AGTGGAGAGCTGAAAGTGGAACAAAACCCTCTGTTCCTGAGCATGCAG TCAGC CGTGCAT
GAGGGAAAAAACTATACCATCTACTGCAATTATTCAACCACTTCAGAC ACCCT GACCTCT
AGACTGTATTGGTACAGGCAGGATCCTGGGAAAAGTCTGGAATCTCTG CCACA CTGCCAC
TTTGTGTTGCTATCAAATGGAGCAGTGAAGCAGGAGGGACGATTAATG TCA CTACTTC
GCCTCACTTGATACCAAAGC TGTGCCG
TGGACA
hTRA hTRA 169 ATGAACTCCTCTCTGGACTTTCTAATTCTGATCTTAATGTTTGGAGGA CCATT TATCAGA
V40 V40 ACCAGCAGCAATTCAGTCAAGCAGACGGGCCAAATAACCGTCTCGGAG GTGAA CTCAGCC
GGAGCATCTGTGACTATGAACTGCACATACACATCCACGGGGTACCCT ATATT GTGTACT
ACCCTTTTCTGGTATGTGGAATACCCCAGCAAACCTCTGCAGCTTCTT CAGTC ACTGTCT
CAGAGAGAGACAATGGAAAACAGCAAAAACTTCGGAGGCGGAAATATT CAGG TCTGGGA
AAAGACAAAAACTCCC GA
hTRA hTRA 170 ATGGTGAAGATCCGGCAATTTTTGTTGGCTATTTTGTGGCTTCAGCTA CTGCA TCCCAGA
V41 V10 AGCTGTGTAAGTGCCGCCAAAAATGAAGTGGAGCAGAGTCCTCAGAAC CATCA GACTCTG
CTGACTGCCCAGGAAGGAGAATTTATCACAATCAACTGCAGTTACTCG CAGCC CCGTCTA
GTAGGAATAAGTGCCTTACACTGGCTGCAACAGCATCCAGGAGGAGGC TCCCA CATCTGT
ATTGTTTCCTTGTTTATGCTGAGCTCAGGGAAGAAGAAGCATGGAAGA GCTGTCA
TTAATTGCCACAATAAACATACAGGAAAAGCACAGCTCC GA
TABLE 16
TCR β chain V segments and binding sites for primers presented in Table 4 and
Table 10. The sequence for each V segments presented in this table
consists of three parts (listed from 5′ to 3′): Sequence
upstream of primer binding site, sequence of the primer
binding site and sequence downstream of the primer binding site.
hTRBV
Primer sequence
binding downstream
V site of primer
segment Primer SEQ within binding
name name ID NO hTRBV sequence upstream of primer binding site hTRBV site
hTRB hTRB 171 ATGGGCTGAAGTCTCCACTGTGGTGTGGTCCATTGTCTCAGGCTCCAT GTGGT CTCAGCT
V01 V01 GGATACTGGAATTACCCAGACACCAAAATACCTGGTCACAGCAATGGG CGCAC GCGTATC
GAGTAAAAGGACAATGAAACGTGAGCATCTGGGACATGATTCTATGTA TGCAG TCTGCAC
TTGGTACAGACAGAAAGCTAAGAAATCCCTGGAGTTCATGTTTTACTA CAAGA CAGCAGC
CAACTGTAAGGAATTCATTGAAAACAAGACTGTGCCAAATCACTTCAC AGA CAAGA
ACCTGAATGCCCTGACAGCTCTCGCTTATACCTTCAT
hTRB hTRB 172 ATGGATACCTGGCTCGTATGCTGGGCAATTTTTAGTCTCTTGAAAGCA GATCC CTCAGCC
V02 V02 GGACTCACAGAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACA GGTCC ATGTACT
CAGATGGGACAGGAAGTGATCTTGCGCTGTGTCCCCATCTCTAATCAC ACAAA TCTGTGC
TTATACTTCTATTGGTACAGACAAATCTTGGGGCAGAAAGTCGAGTTT GCTGG CAGCAGT
CTGGTTTCCTTTTATAATAATGAAATCTCAGAGAAGTCTGAAATATTC AGGA GAAGC
GATGATCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTG
AA
hTRB hTRB 173 ATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCTCCAAGCA CATCA CTCTGCT
V03-1 V03-1 GGTCCCTTGGACACAGCTGTTTCCCAGACTCCAAAATACCTGGTCACA ATTCC GTGTATT
CAGATGGGAAACGACAAGTCCATTAAATGTGAACAAAATCTGGGCCAT CTGGA TCTGTGC
GATACTATGTATTGGTATAAACAGGACTCTAAGAAATTTCTGAAGATA GCTTG CAGCAGC
ATGTTTAGCTACAATAATAAGGAGCTCATTATAAATGAAACAGTTCCA GTGA CAAGA
AATCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCA
hTRB hTRB 174 ATGGGCTGCAGGCTCCTCTGCTATGTGGCCCTCTGCCTCCTGCAAGCA CATCA CTCTGCT
V03-2 V03-1 GGATCCACTGGACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCAC ATTCC GTGTATT
ACAGATGGGAAAAAAGGAGTCTCTTAAATGAGAACAAAATCTGGGCCA CTGGA TCTGTGC
TAATGCTATGTATTGGTATAAACAGGACTCTAAGAAATTTCTGAAGAC GCTTG CAGCAGC
AATGTTTATCTACAGTAACAAGGAGCCAATTTTAAATGAAACAGTTCC GTGA CAAGA
AAATCGCTTCTCACCTGACTCTCCAGACAAAGCTCATTTAAATCTTCA
hTRB hTRB 175 ATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCTGTCTCCTGGGAGCA TTCAC AAGACTC
V04-1 V04-1 GTTCCCATAGACACTGAAGTTACCCAGACACCAAAACACCTGGTCATG CTACA AGCCCTG
GGAATGACAAATAAGAAGTCTTTGAAATGTGAACAACATATGGGGCAC CGCCC TATCTCT
AGGGCTATGTATTGGTACAAGCAGAAAGCTAAGAAGCCACCGGAGCTC TGCAG GCGCCAG
ATGTTTGTCTACAGCTATGAGAAACTCTCTATAAATGAAAGTGTGCCA CCAG CAGCCAA
AGTCGCTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACC GA
hTRB hTRB 176 ATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCTGTCTCCTGGGAGCG TTCAC AAGACTC
V04-2 V04-2 GTCCCCATGGAAACGGGAGTTACGCAGACACCAAGACACCTGGTCATG CTACA GGCCCTG
GGAATGACAAATAAGAAGTCTTTGAAATGTGAACAACATCTGGGGCAT CACCC TATCTCT
AACGCTATGTATTGGTACAAGCAAAGTGCTAAGAAGCCACTGGAGCTC TGCAG GTGCCAG
ATGTTTGTCTACAACTTTAAAGAACAGACTGAAAACAACAGTGTGCCA CCAG CAGCCAA
AGTCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCC GA
hTRB hTRB 177 ATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCTGTCTCCTGGGAGCG TTCAC AAGACTC
V04-3 V04-2 GGTGAGTTGGTCCCCATGGAAACGGGAGTTACGCAGACACCAAGACAC CTACA GGCCCTG
CTGGTCATGGGAATGACAAATAAGAAGTCTTTGAAATGTGAACAACAT CACCC TATCTCT
CTGGGTCATAACGCTATGTATTGGTACAAGCAAAGTGCTAAGAAGCCA TGCAG GCGCCAG
CTGGAGCTCATGTTTGTCTACAGTCTTGAAGAACGGGTTGAAAACAAC CCAG CAGCCAA
AGTGTGCCAAGTCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTA GA
TTCC
hTRB hTRB 178 ATGGGCTCCAGGCTGCTCTGTTGGGTGCTGCTTTGTCTCCTGGGAGCA GAATG GGGACTC
V05-1 V05-1 GGCCCAGTAAAGGCTGGAGTCACTCAAACTCCAAGATATCTGATCAAA TGAGC GGCCCTT
ACGAGAGGACAGCAAGTGACACTGAGCTGCTCCCCTATCTCTGGGCAT ACCTT TATCTTT
AGGAGTGTATCCTGGTACCAACAGACCCCAGGACAGGGCCTTCAGTTC GGAGC GCGCCAG
CTCTTTGAATACTTCAGTGAGACACAGAGAAACAAAGGAAACTTCCCT TGG CAGCTTG
GGTCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGAT G
hTRB hTRB 179 ATGGGCTCCGGACTCCTCTGCTGGACGCTGCTTTGTTTCCTGGGAGCA TACTG GGACTCA
V05-2 V05-2 GGCCCAGTGGAGGCTGGAATCACCCAAGCTCCAAGACACCTGATCAAA AGTCA GCCCTGT
ACAAGAGACCAGCAAGTGACACTGAGATGCTCCCCTGCCTCTGGGCAT AACAC ATCTCTG
AACTGTGTGTCCTGGTACCTACGAACTCCAAGTCAGCCCCTCTAGTTA
TTGTTACAATATTGTAATAGGTTACAAAGAGCAAAAGGAAACTTGCCT GGAGC TGCCAGC
AATTGATTCTCAGCTCACCACGTCCATAACTAT TAGG AACTTG
hTRB hTRB 180 ATGGGCCCCGGGCTCCTCTGCTGGGAACTGCTTTATCTCCTGGGAGCA GCTCT TGGAGCT
V05-3 V05-3 GGCCCAGTGGAGGCTGGAGTCACCCAAAGTCCCACACACCTGATCAAA GAGAT GGGGGAC
ACGAGAGGACAGCAAGTGACTCTGAGATGCTCTCCTATCTCTGGGCAC GAATG TCGGCCC
AGCAGTGTGTCCTGGTACCAACAGGCCCCGGGTCAGGGGCCCCAGTTT TGAGT TGTATCT
ATCTTTGAATATGCTAATGAGTTAAGGAGATCAGAAGGAAACTTCCCT GCCT CTGTGCC
AATCGATTCTCAGGGCGCCAGTTCCATGACTGTT AGAAGCT
TGG
hTRB hTRB 181 ATGGGCCCTGGGCTCCTCTGCTGGGTGCTGCTTTGTCTCCTGGGAGCA CTGAG GGAGCTG
V05-4 V05-4 GGCTCAGTGGAGACTGGAGTCACCCAAAGTCCCACACACCTGATCAAA CTGAA GACGACT
ACGAGAGGACAGCAAGTGACTCTGAGATGCTCTTCTCAGTCTGGGCAC TGTGA CGGCCCT
AACACTGTGTCCTGGTACCAACAGGCCCTGGGTCAGGGGCCCCAGTTT ACGCC GTATCTC
ATCTTTCAGTATTATAGGGAGGAAGAGAATGGCAGAGGAAACTTCCCT TT TGTGCCA
CCTAGATTCTCAGGTCTCCAGTTCCCTAATTATAGCT GCAGCTT
GG
hTRB hTRB 182 ATGGGCCCTGGGCTCCTCTGCTGGGTGCTGCTTTGTCTCCTGGGAGCA CTGAG GTTGCTG
V05-5 V05-4 GGCCCAGTGGACGCTGGAGTCACCCAAAGTCCCACACACCTGATCAAA CTGAA GGGGACT
ACGAGAGGACAGCAAGTGACTCTGAGATGCTCTCCTATCTCTGGGCAC TGTGA CGGCCCT
AAGAGTGTGTCCTGGTACCAACAGGTCCTGGGTCAGGGGCCCCAGTTT ACGCC GTATCTC
ATCTTTCAGTATTATGAGAAAGAAGAGAGAGGAAGAGGAAACTTCCCT TT TGTGCCA
GATCGATTCTCAGCTCGCCAGTTCCCTAACTATAGCT GCAGCTT
GG
hTRB hTRB 183 ATGGGCCCCGGGCTCCTCTGCTGGGCACTGCTTTGTCTCCTGGGAGCA CTGAG GTTGCTG
V05-6 V05-4 GGCTTAGTGGACGCTGGAGTCACCCAAAGTCCCACACACCTGATCAAA CTGAA GGGGACT
ACGAGAGGACAGCAAGTGACTCTGAGATGCTCTCCTAAGTCTGGGCAT TGTGA CGGCCCT
GACACTGTGTCCTGGTACCAACAGGCCCTGGGTCAGGGGCCCCAGTTT ACGCC CTATCTC
ATCTTTCAGTATTATGAGGAGGAAGAGAGACAGAGAGGCAACTTCCCT TT TGTGCCA
GATCGATTCTCAGGTCACCAGTTCCCTAACTATAGCT GCAGCTT
GG
hTRB hTRB 184 ATGGGCCCCGGGCTCCTCTGCTGGGTGCTGCTTTGTCCCCTAGGAGAA CTGAG GTTGCTA
V05-7 V05-4 GGCCCAGTGGACGCTGGAGTCACCCAAAGTCCCACACACCTGATCAAA CTGAA GGGGACT
ACGAGAGGACAGCACGTGACTCTGAGATGCTCTCCTATCTCTGGGCAC TGTGA CGGCCCT
ACCAGTGTGTCCTCGTACCAACAGGCCCTGGGTCAGGGGCCCCAGTTT ACGCC CTATCTC
ATCTTTCAGTATTATGAGAAAGAAGAGAGAGGAAGAGGAAACTTCCCT TT TGTGCCA
GATCAATTCTCAGGTCACCAGTTCCCTAACTATAGCT GCAGCTT
GG
hTRB hTRB 185 ATGGGACCCAGGCTCCTCTTCTGGGCACTGCTTTGTCTCCTCGGAACA CTGAG GGAGCTG
V05-8 V05-4 GGCCCAGTGGAGGCTGGAGTCACACAAAGTCCCACACACCTGATCAAA CTGAA GAGGACT
ACGAGAGGACAGCAAGCGACTCTGAGATGCTCTCCTATCTCTGGGCAC TGTGA CGGCCCT
ACCAGTGTGTACTGGTACCAACAGGCCCTGGGTCTGGGCCTCCAGTTC ACGCC GTATCTC
CTCCTTTGGTATGACGAGGGTGAAGAGAGAAACAGAGGAAACTTCCCT TT TGTGCCA
CCTAGATTTTCAGGTCGCCAGTTCCCTAATTATAGCT GCAGCTT
GG
hTRB hTRB 186 ATGAGCATCGGGCTCCTGTGCTGTGTGGCCTTTTCTCTCCTGTGGGCA GAGTT CGGCTGC
V06-1 V06-1 AGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCAGGTCCTG CTCGC TCCCTCC
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT TCAGG CAGACAT
AACTCCATGTACTGGTATCGACAAGACCCAGGCATGGGACTGAGGCTG CTGGA CTGTGTA
ATTTATTACTCAGCTTCTGAGGGTACCACTGACAAAGGAGAAGTCCCC GT CTTCTGT
AATGGCTACAATGTCTCCAGATTAAACAAACGG GCCAGCA
GTGAAGC
hTRB hTRB 187 ATGAGCCTCGGGCTCCTGTGCTGTGGGGCCTTTTCTCTCCTGTGGGCA CTGGG CCTCCCA
V06-2 V06-2 GGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCGGGTCCTG GTTGG AACATCT
AAGACAGGACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCAT AGTCG GTGTACT
GAATACATGTACTGGTATCGACAAGACCCAGGCATGGGGCTGAGGCTG GCTGC TCTGTGC
ATTCATTACTCAGTTGGTGAGGGTACAACTGCCAAAGGAGAGGTCCCT TC CAGCAGT
GATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTG TACTC
hTRB hTRB 188 ATGAGCCTCGGGCTCCTGTGCTGTGGGGCCTTTTCTCTCCTGTGGGCA CTGGG CCTCCCA
V06-3 V06-2 GGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCGGGTCCTG GTTGG AACATCT
AAGACAGGACAGAGCATGACACTGCTGTGTGCCCAGGATATGAACCAT AGTCG GTGTACT
GAATACATGTACTGGTATCGACAAGACCCAGGCATGGGGCTGAGGCTG GCTGC TCTGTGC
ATTCATTACTCAGTTGGTGAGGGTACAACTGCCAAAGGAGAGGTCCCT TC CAGCAGT
GATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTG TACTC
hTRB hTRB 189 ATGAGAATCAGGCTCCTGTGCTGTGTGGCCTTTTCTCTCCTGTGGGCA CCCCT TACCCTC
V06-4 V06-4 GGTCCAGTGATTGCTGGGATCACCCAGGCACCAACATCTCAGATCCTG CACGT TCAGACA
GCAGCAGGACGGCGCATGACACTGAGATGTACCCAGGATATGAGACAT TGGCG TCTGTGT
AATGCCATGTACTGGTATAGACAAGATCTAGGACTGGGGCTAAGGCTC TCTGC ACTTCTG
ATCCATTATTCAAATACTGCAGGTACCACTGGCAAAGGAGAAGTCCCT TG TGCCAGC
GATGGTTATAGTGTCTCCAGAGCAAACACAGATGATTTC AGTGACT
C
hTRB hTRB 190 ATGAGCATCGGCCTCCTGTGCTGTGCAGCCTTGTCTCTCCTGTGGGCA TCCCG TGCTCCC
V06-5 V06-5 GGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCAGGTCCTG CTCAG TCCCAGA
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT GCTGC CATCTGT
GAATACATGTCCTGGTATCGACAAGACCCAGGCATGGGGCTGAGGCTG TGTCG GTACTTC
ATTCATTACTCAGTTGGTGCTGGTATCACTGACCAAGGAGAAGTCCCC GC TGTGCCA
AATGGCTACAATGTCTCCAGATCAACCACAGAGGATT GCAGTTA
CTC
hTRB hTRB 191 ATGAGCATCAGCCTCCTGTGCTGTGCAGCCTTTCCTCTCCTGTGGGCA GATTT TGGCTGC
V06-6 V06-6 GGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCGCATCCTG CCCGC TCCCTCC
AAGATAGGACAGAGCATGACACTGCAGTGTACCCAGGATATGAACCAT TCAGG CAGACAT
AACTACATGTACTGGTATCGACAAGACCCAGGCATGGGGCTGAAGCTG CTGGA CTGTGTA
ATTTATTATTCAGTTGGTGCTGGTATCACTGATAAAGGAGAAGTCCCG GT CTTCTGT
AATGGCTACAACGTCTCCAGATCAACCACAGAG GCCAGCA
GTTACTC
hTRB hTRB 192 ATGAGCCTCGGGCTCCTGTGCTGTGTGGCCTTTTCTCTCCTGTGGGCA TCCCC GCTCCCT
V06-7 V06-7 GGTCCAATGAATGCTGGTGTCACTCAGACCCCAAAATTCCACGTCCTG CTCAA CTCAGAC
AAGACAGGACAGAGCATGACTCTGCTGTGTGCCCAGGATATGAACCAT GCTGG TTCTGTTT
GAATACATGTATCGGTATCGACAAGACCCAGGCAAGGGGCTGAGGCTG AGTCA ACTTCTG
ATTTACTACTCAGTTGCTGCTGCTCTCACTGACAAAGGAGAAGTTCCC GCT TGCCAGC
AATGGCTACAATGTCTCCAGATCAAACACAGAGGATT AGTTACT
C
hTRB hTRB 193 ATGAGCCTCGGGCTCCTGTGCTGTGCGGCCTTTTCTCTCCTGTGGGCA TCCCA TGCTCCC
V06-8 V06-8 GGTCCCGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCACATCCTG CTCAG TCCCAGA
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT GCTGG CATCTGT
GGATACATGTCCTGGTATCGACAAGACCCAGGCATGGGGCTGAGACTG TGTCG GTACTTG
ATTTACTACTCAGCTGCTGCTGGTACTACTGACAAAGAAGTCCCCAAT GC TGTGCCA
GGCTACAATGTCTCTAGATTAAACACAGAGGATT GCAGTTA
CTC
hTRB hTRB 194 ATGAGCATCGGGCTCCTGTGCTGTGTGGCCTTTTCTCTCCTGTGGGAG GATTT CAGCTGC
V06-9 V06-6 GGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCACATCCTG CCCGC TCCCTCC
AAGACAGGACAGAGCATGACACTGCAGTGTGCCCAGGATATGAACCAT TCAGG CAGACAT
GGATACTTGTCCTGGTATCGACAAGACCCAGGCATGGGGCTGAGGCGC CTGGA CTGTATA
ATTCATTACTCAGTTGCTGCTGGTATCACTGACAAAGGAGAAGTCCCC GT CTTCTGT
GATGGCTACAATGTATCCAGATCAAACACAGAG GCCAGCA
GTTATTC
hTRB hTRB 195 ATGGGCACAAGGCTCCTCTGCTGGGCAGCCATATGTCTCCTGGGGGCA CTCTG GCAGGGG
V07-1 V07-1 GATCACACAGGTGCTGGAGTCTCCCAGTCCCTGAGACACAAGGTAGCA AAGTT GACTTGG
AAGAAGGGAAAGGATGTAGCTCTCAGATATGATCCAATTTCAGGTCAT CCAGC CTGTGTA
AATGCCCTTTATTGGTACCGACAGAGCCTGGGGCAGGGCCTGGAGTTT GCACA TCTCTGT
CCAATTTACTTCCAAGGCAAGGATGCAGCAGACAAATCGGGGCTTCCC CA GCCAGCA
CGTGATCGGTTCTCTGCACAGAGGTCTGAGGGATCCATCTCCA GCTCAGC
hTRB hTRB 196 ATGGGCACCAGGCTCCTCTTCTGGGTGGCCTTCTGTCTCCTGGGGGCA GATCC GACTCGG
V07-2 V07-2 GATCACACAGGAGCTGGAGTCTCCCAGTCCCCCAGTAACAAGGTCACA AGCGC CCGTGTA
GAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCAGGTCAT ACACA TCTCTGT
ACTGCCCTTTACTGGTACCGACAGAGCCTGGGGCAGGGCCTGGAGTTT GCAGG GCCAGCA
TTAATTTACTTCCAAGGCAACAGTGCACCAGACAAATCAGGGCTGCCC AG GCTTAGC
AGTGATCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTG
AC
hTRB hTRB 197 ATGGGCACCAGGCTCCTCTGCTGGGCAGCCCTGTGCCTCCTGGGGGCA ACTCT GCGGGGG
V07-3 V07-5 GATCACACAGGTGCTGGAGTCTCCCAGACCCCCAGTAACAAGGTCACA GAAGA GACTCAG
GAGAAGGGAAAATATGTAGAGCTCAGGTGTGATCCAATTTCAGGTCAT TCCAG CCGTGTA
ACTGCCCTTTACTGGTACCGACAAAGCCTGGGGCAGGGCCCAGAGTTT CGCAC TCTCTGT
CTAATTTACTTCCAAGGCACGGGTGCGGCAGATGACTCAGGGCTGCCC AGA GCCAGCA
AACGATCGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCT GCTTAAC
hTRB hTRB 198 ATGGGCACCAGGCTCCTCTGCTGGGTGGTCCTGGGTTTCCTAGGGACA ACTCT GCAGGGG
V07-4 V07-5 GATCACACAGGTGCTGGAGTCTCCCAGTCCCCAAGGTACAAAGTCGCA GAAGA GACTCAG
AAGAGGGGACGGGATGTAGCTCTCAGGTGTGATTCAATTTCGGGTCAT TCCAG CTGTGTA
GTAACCCTTTATTGGTACCGACAGACCCTGGGGCAGGGCTCAGAGGTT CGCAC TCTCTGT
CTGACTTACTCCCAGAGTGATGCTCAACGAGACAAATCAGGGCGGCCC AGA GCCAGCA
AGTGGTCGGTTCTCTGCAGAGAGGCCTGAGAGATCCGTCTCC GCTTAGC
hTRB hTRB 199 ATGGGCACCAGGCTCCTCTGCTGGGTGGTCCTGGGTTTCCTAGGGACA AGATC GCGACTC
V07-5 V07-5 GATCACACAGGTGCTGGAGTCTCCCAGTCCCCAAGGTACGAAGTCACA CAGCG GGCTGTG
CAGAGGGGACAGGATGTAGCTCCCAGGTGTGATCCAATTTCGGGTCAG CACAG TATCTCT
GTAACCCTTTATTGGTACCGACAGACCCTGGGGCAGGGCCAAGAGTTT AGCAA GTGCCAG
CTGACTTCCTTCCAGGATGAAACTCAACAAGATAAATCAGGGCTGCTC GG AAGCTTA
AGTGATCAATTCTCCACAGAGAGGTCTGAGGATCTTTCTCCACCTGA G
hTRB hTRB 200 ATGGGCACCAGTCTCCTATGCTGGGTGGTCCTGGGTTTCCTAGGGACA CAGCG CGGCCAT
V07-6 V07-6 GATCACACAGGTGCTGGAGTCTCCCAGTCTCCCAGGTACAAAGTCACA CACAG GTATCGC
AAGAGGGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT AGCAG TGTGCCA
GTATCCCTTTATTGGTACCGACAGGCCCTGGGGCAGGGCCCAGAGTTT CGGGA GCAGCTT
CTGACTTACTTCAATTATGAAGCCCAACAAGACAAATCAGGGCTGCCC CT AGC
AATGATCGGTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTG
ACGATC
hTRB hTRB 201 ATGGGTACCAGTCTCCTATGCTGGGTGGTCCTGGGTTTCCTAGGGACA CAGCG CAGCCAT
V07-7 V07-6 GATCACACAGGTGCTGGAGTCTCCCAGTCTCCCAGGTACAAAGTCACA CACAG GTATCGC
AAGAGGGGACAGGATGTAACTCTCAGGTGTGATCCAATTTCGAGTCAT AGCAG TGTGCCA
GCAACCCTTTATTGGTATCAACAGGCCCTGGGGCAGGGCCCAGAGTTT CGGGA GCAGCTT
CTGACTTACTTCAATTATGAAGCTCAACCAGACAAATCAGGGCTGCCC CT AGC
AGTGATCGGTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTG
ACGATT
hTRB hTRB 202 ATGGGCACCAGGCTCCTCTGCTGGGTGGTCCTGGGTTTCCTAGGGACA GATCC GACTCCG
V07-8 V07-2 GATCACACAGGTGCTGGAGTCTCCCAGTCCCCTAGGTACAAAGTCGCA AGCGC CCGTGTA
AAGAGAGGACAGGATGTAGCTCTCAGGTGTGATCCAATTTCGGGTCAT ACACA TCTCTGT
GTATCCCTTTTTTGGTACCAACAGGCCCTGGGGCAGGGGCCAGAGTTT GCAGG GCCAGCA
CTGACTTATTTCCAGAATGAAGCTCAACTAGACAAATCGGGGCTGCCC AG GCTTAGC
AGTGATCGCTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTG
AA
hTRB hTRB 203 ATGGGCACCAGCCTCCTCTGCTGGATGGCCCTGTGTCTCCTGGGGGCA GAGAT GGGACTC
V07-9 V07-9 GATCACGCAGATACTGGAGTCTCCCAGAACCCCAGACACAAGATCACA CCAGC GGCCATG
AAGAGGGGACAGAATGTAACTTTCAGGTGTGATCCAATTTCTGAACAC GCACA TATCTCT
AACCGCCTTTATTGGTACCGACAGACCCTGGGGCAGGGCCCAGAGTTT GAGCA GTGCCAG
CTGACTTACTTCCAGAATGAAGCTCAACTAGAAAAATCAAGGCTGCTC GG CAGCTTA
AGTGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTG GC
hTRB hTRB 204 GAGGCAGGGATCAGCCAGATACCAAGATATCACAGACACACAGGGAAA CCCTC GCACCAG
V08-1 V08-1 AAGATCATCCTGAAATATGCTCAGATTAGGAACCATTATTCAGTGTTC AACCC CCAGACC
TGTTATCAATAAGACCAAGAATAGGGGCTGAGGCTGATCCATTATTCA TGGAG TCTGTAC
GGTAGTATTGGCAGCATGACCAAAGGCGGTGCCAAGGAAGGGTACAAT TCTAC CTCTGTG
GTCTCTGGAAACAAGCTCAAGCATTTT TA GCAGTGC
ATC
hTRB hTRB 205 ATGAACCCCAAACTCTTCTGTGTGACCCTTTGTCTCCTGGGAGCAGGC TCCCC GCACCAG
V08-2 V08-2 TCTATTGATGCTGGGATCACCCAGATGCCAAGATATCACATTGTACAG AATCC CCAGACC
AAGAAAGAGATGATCCTGGAATGTGCTCAGGTTAGGAACAGTGTTCTG TGGCA TATCTGT
ATATCGACAGGACCCAAGACGGGGGCTGAAGCTTATCCACTATTCAGG TCCAC ACCACTG
CAGTGGTCACAGCAGGACCAAAGTTGATGTCACAGAGGGGTACTGTGT CA TGGCAGC
TTCTTGAAACAAGCTTGAGCATT ACATC
hTRB hTRB 206 ATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCCTGGGAGCA CTAAA GGGGGAC
V09 V09 GGCCCAGTGGATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACA CCTGA TCAGCTT
GCAACTGGACAGCGAGTGACGCTGAGATGCTCCCCTAGGTCTGGAGAC GCTCT TGTATTT
CTCTCTGTGTACTGGTACCAACAGAGCCTGGACCAGGGCCTCCAGTTC CTGGA CTGTGCC
CTCATTCAGTATTATAATGGAGAAGAGAGAGCAAAAGGAAACATTCTT GCT AGCAGCG
GAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAA TAG
hTRB hTRB 207 ATGGGCACGAGGCTCTTCTTCTATGTGGCCCTTTGTCTGCTGTGGGCA CCCTC CTCCTCC
V10-1 V10-1 GGACACAGGGATGCTGAAATCACCCAGAGCCCAAGACACAAGATCACA ACTCT CAGACAT
GAGACAGGAAGGCAGGTGACCTTGGCGTGTCACCAGACTTGGAACCAC GGAGT CTGTATA
AACAATATGTTCTGGTATCGACAAGACCTGGGACATGGGCTGAGGCTG CTGCT TTTCTGC
ATCCATTACTCATATGGTGTTCAAGACACTAACAAAGGAGAAGTCTCA GC GCCAGCA
GATGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCC GTGAGTC
hTRB hTRB 208 ATGGGCACCAGGCTCTTCTTCTATGTGGCCCTTTGTCTGCTGTGGGCA CCCTC CCGCTCC
V10-2 V10-2 GGACACAGGGATGCTGGAATCACCCAGAGCCCAAGATACAAGATCACA ACTCT CAGACAT
GAGACAGGAAGGCAGGTGACCTTGATGTGTCACCAGACTTGGAGCCAC GGAGT CTGTGTA
AGCTATATGTTCTGGTATCGACAAGACCTGGGACATGGGCTGAGGCTG CAGCT TTTCTGC
ATCTATTACTCAGCAGCTGCTGATATTACAGATAAAGGAGAAGTCCCC AC GCCAGCA
GATGGCTATGTTGTCTCCAGATCCAAGACAGAGAATTTCC GTGAGTC
hTRB hTRB 209 ATGGGCACAAGGTTGTTCTTCTATGTGGCCCTTTGTCTCCTGTGGACA TCCTC CAGCTCC
V10-3 V10-3 GGACACATGGATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACA ACTCT CAGACAT
GAGACAGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCAC GGAGT CTGTGTA
CGCTATATGTACTGGTATCGACAAGACCCGGGGCATGGGCTGAGGCTG CCGCT CTTCTGT
ATCCATTACTCATATGGTGTTAAAGATACTGACAAAGGAGAAGTCTCA AC GCCATCA
GATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCC GTGAGTC
hTRB hTRB 210 ATGAGCACCAGGCTTCTCTGCTGGATGGCCCTCTGTCTCCTGGGGGCA CCACT GAGCTTG
V11-1 V11-1 GAACTCTCAGAAGCTGAAGTTGCCCAGTCCCCCAGATATAAGATTACA CTCAA GGGACTC
GAGAAAAGCCAGGCTGTGGCTTTTTGGTGTGATCCTATTTCTGGCCAT GATCC GGCCATG
GCTACCCTTTACTGGTACCGGCAGATCCTGGGACAGGGCCCGGAGCTT AGCCT TATCTCT
CTGGTTCAATTTCAGGATGAGAGTGTAGTAGATGATTCACAGTTGCCT GCA GTGCCAG
AAGGATCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACT CAGCTTA
GC
hTRB hTRB 211 ATGGGCACCAGGCTCCTCTGCTGGGCGGCCCTCTGTCTCCTGGGAGCA CCACT AAGCTTG
V11-2 V11-1 GAACTCACAGAAGCTGGAGTTGCCCAGTCTCCCAGATATAAGATTATA CTCAA AGGACTC
GAGAAAAGGCAGAGTGTGGCTTTTTGGTGCAATCCTATATCTGGCCAT GATCC GGCCGTG
GCTACCCTTTACTGGTACCAGCAGATCCTGGGACAGGGCCCAAAGCTT AGCCT TATCTCT
CTGATTCAGTTTCAGAATAACGGTGTAGTGGATGATTCACAGTTGCCT GCA GTGCCAG
AAGGATCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACT CAGCTTA
GA
hTRB hTRB 212 ATGGGTACCAGGCTCCTCTGCTGGGTGGCCTTCTGTCTCCTGGTGGAA CCACT GAGCTTG
V11-3 V11-1 GAACTCATAGAAGCTGGAGTGGTTCAGTCTCCCAGATATAAGATTATA CTCAA GGGACTC
GAGAAAAAACAGCCTGTGGCTTTTTGGTGCAATCCTATTTCTGGCCAC GATCC GGCCGTG
AATACCCTTTACTGGTACCTGCAGAACTTGGGACAGGGCCCGGAGCTT AGCCT TATCTCT
CTGATTCGATATGAGAATGAGGAAGCAGTAGACGATTCACAGTTGCCT GCA GTGCCAG
AAGGATCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACT CAGCTTA
GA
hTRB hTRB 213 ATGGGCTCTTGGACCCTCTGTGTGTCCCTTTATATCCTGGTAGCGACA GAGGA GGGACTT
V12-1 V12-1 CACACAGATGCTGGTGTTATCCAGTCACCCAGGCACAAAGTGACAGAG TCCAG GGGCCTA
ATGGGACAATCAGTAACTCTGAGATGCGAACCAATTTCAGGCCACAAT CCCAT TATTTCT
GATCTTCTCTGGTACAGACAGACCTTTGTGCAGGGACTGGAATTGCTG GGAAC GTGCCAG
AATTACTTCTGCAGCTGGACCCTCGTAGATGACTCAGGAGTGTCCAAG CCA CAGCTTT
GATTGATTCTCAGCACAGATGCCTGATGTATCATTCTCCACTCT GC
hTRB hTRB 214 ATGGACTCCTGGACCCTCTGTGTGTCCCTTTGTATCCTGGTAGCGACA CTGAA AGGGGGA
V12-2 V12-2 TGCACAGATGCTGGCATTATCCAGTCACCCAAGCATGAGGTGACAGAA GATCC CTCGGCC
ATGGGACAAACAGTGACTCTGAGATGTGAGCCAATTTTTGGCCACAAT AGCCT GTGTATG
TTCCTTTTCTGGTACAGAGATACCTTCGTGCAGGGACTGGAATTGCTG GCAGA TCTGTGC
AGTTACTTCCGGAGCTGATCTATTATAGATAATGCAGGTATGCCCACA GC AAGTCGC
GAGCGATTCTCAGCTGAGAGGCCTGATGGATCATTCTCTACT TTAGC
hTRB hTRB 215 ATGGACTCCTGGACCTTCTGCTGTGTGTCCCTTTGCATCCTGGTAGCG CAGCC CAGCTGT
V12-3 V12-3 AAGCATACAGATGCTGGAGTTATCCAGTCACCCCGCCATGAGGTGACA CTCAG GTACTTC
GAGATGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGCCAC AACCC TGTGCCA
AACTCCCTTTTCTGGTACAGACAGACCATGATGCGGGGACTGGAGTTG AGGGA GCAGTTT
CTCATTTACTTTAACAACAACGTTCCGATAGATGATTCAGGGATGCCC CT AGC
GAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTG
AAGATC
hTRB hTRB 216 ATGGGCTCCTGGACCCTCTGCTGTGTGTCCCTTTGCATCCTGGTAGCA CAGCC CAGCTGT
V12-4 V12-3 AAGCACACAGATGCTGGAGTTATCCAGTCACCCCGGCACGAGGTGACA CTCAG GTACTTC
GAGATGGGACAAGAAGTGACTCTGAGATGTAAACCAATTTCAGGACAC AACCC TGTGCCA
GACTACCTTTTCTGGTACAGACAGACCATGATGCGGGGACTGGAGTTG AGGGA GCAGTTT
CTCATTTACTTTAACAACAACGTTCCGATAGATGATTCAGGGATGCCC CT AGC
GAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTG
AAGATC
hTRB hTRB 217 ATGGCCACCAGGCTCCTCTGCTGTGTGGTTCTTTGTCTCCTGGGAGAA CAGCC CAGCTGT
V12-5 V12-3 GAGCTTATAGATGCTAGAGTCACCCAGACACCAAGGCACAAGGTGACA CTCAG GTATTTT
GAGATGGGACAAGAAGTAACAATGAGATGTCAGCCAATTTTAGGCCAC AACCC TGTGCTA
AATACTGTTTTCTGGTACAGACAGACCATGATGCAAGGACTGGAGTTG AGGGA GTGGTTT
CTGGCTTACTTCCGCAACCGGGCTCCTCTAGATGATTCGGGGATGCCG CT GGT
AAGGATCGATTCTCAGCAGAGATGCCTGATGCAACTTTAGCCACTCTG
AAGATC
hTRB hTRB 218 ATGCTTAGTCCTGACCTGCCTGACTCTGCCTGGAACACCAGGCTCCTC GAGCT CAGCCCT
V13 V13 TGCCATGTCATGCTTTGTCTCCTGGGAGCAGTTTCAGTGGCTGCTGGA CCTTG GTACTTC
GTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGAAACAGCC GAGCT TGTGCCA
ACTCTGAAATGCTATCCTATCCCTAGACACGACACTGTCTACTGGTAC GGGGG GCAGCTT
CAGCAGGGTCCAGGTCAGGACCCCCAGTTCCTCATTTCGTTTTATGAA ACT AGG
AAGATGCAGAGCGATAAAGGAAGCATCCCTGATCGATTCTCAGCTCAA
CAGTTCAGTGACTATCATTCTGAACTGAACAT
hTRB hTRB 219 ATGGTTTCCAGGCTTCTCAGTTTAGTGTCCCTTTGTCTCCTGGGAGCA GGTGC GATTCTG
V14 V14 AAGCACATAGAAGCTGGAGTTACTCAGTTCCCCAGCCACAGCGTAATA AGCCT GAGTTTA
GAGAAGGGCCAGACTGTGACTCTGAGATGTGACCCAATTTCTGGACAT GCAGA TTTCTGT
GATAATCTTTATTGGTATCGACGTGTTATGGGAAAAGAAATAAAATTT ACTGG GCCAGCA
CTGTTACATTTTGTGAAAGAGTCTAAACAGGATGAGTCCGGTATGCCC AG GCCAAGA
AACAATCGATTCTTAGCTGAAAGGACTGGAGGGACGTATTCTACTCTG
AA
hTRB hTRB 220 ATGGGTCCTGGGCTTCTCCACTGGATGGCCCTTTGTCTCCTTGGAACA GACAT GGGACAC
V15 V15 GGTCATGGGGATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACC CCGCT AGCCATG
CAGTTTGGAAAGCCAGTGACCCTGAGTTGTTCTCAGACTTTGAACCAT CACCA TACCTGT
AACGTCATGTACTGGTACCAGCAGAAGTCAAGTCAGGCCCCAAAGCTG GGCCT GTGCCAC
CTGTTCCACTACTATGACAAAGATTTTAACAATGAAGCAGACACCCCT GG CAGCAGA
GATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTT GA
hTRB hTRB 221 ATGAGCCCAATATTCACCTGCATCACAATCCTTTGTCTGCTGGCTGCA TGAGA GAGGATT
V16 V16 GGTTCTCCTGGTGAAGAAGTCGCCCAGACTCCAAAACATCTTGTCAGA TCCAG CAGCAGT
GGGGAAGGACAGAAAGCAAAATTATATTGTGCCCCAATAAAAGGACAC GCTAC GTATTTT
AGTTATGTTTTTTGGTACCAACAGGTCCTGAAAAACGAGTTCAAGTTC GAAGC TGTGCCA
TTGATTTCCTTCCAGAATGAAAATGTCTTTGATGAAACAGGTATGCCC TT GCAGCCA
AAGGAAAGATTTTCAGCTAAGTGCCTCCCAAATTCACCCTGTAGCCT ATC
hTRB hTRB 222 ATGGATATCTGGCTCCTCTGCTGGGTGACCCTGTGTCTCTTGGCGGCA GAAGA AGGGACT
V17 V17 GGACACTCGGAGCCTGGAGTCAGCCAGACCCCCAGACACAAGGTCACC TCCAT CAGCCGT
AACATGGGACAGGAGGTGATTCTGAGGTGCGATCCATCTTCTGGTCAC CCCGC GTATCTC
ATGTTTGTTCACTGGTACCGACAGAATCTGAGGCAAGAAATGAAGTTG AGAGC TACAGTA
CTGATTTCCTTCCAGTACCAAAACATTGCAGTTGATTCAGGGATGCCC CG GCGGTGG
AAGGAACGATTCACAGCTGAAAGACCTAACGGAACGTCTTCCACGCT
hTRB hTRB 223 ATGGACACCAGAGTACTCTGCTGTGCGGTCATCTGTCTTCTGGGGGCA GGATC AGATTCG
V18 V18 GGTCTCTCAAATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGG CAGCA GCAGCTT
AGGAGGGGACAGGAGGCAAGACTGAGATGCAGCCCAATGAAAGGACAC GGTAG ATTTCTG
AGTCATGTTTACTGGTATCGGCAGCTCCCAGAGGAAGGTCTGAAATTC TGCGA TGCCAGC
ATGGTTTATCTCCAGAAAGAAAATATCATAGATGAGTCAGGAATGCCA GG TCACCAC
AAGGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTG C
A
hTRB hTRB 224 ATGAGCAACCAGGTGCTCTGCTGTGTGGTCCTTTGTTTCCTGGGAGCA CACTG AACCCGA
V19 V19 AACACCGTGGATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGA TGACA CAGCTTT
AAGGAAGGACAGAATGTGACCCTGAGTTGTGAACAGAATTTGAACCAC TCGGC CTATCTC
GATGCCATGTACTGGTACCGACAGGACCCAGGGCAAGGGCTGAGATTG CCAAA TGTGCCA
ATCTACTACTCACAGATAGTAAATGACTTTCAGAAAGGAGATATAGCT AG GTAGTAT
GAAGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCT AGA
hTRB hTRB 225 ATGCTGCTGCTTCTGCTGCTTCTGGGGCCAGGTATAAGCCTCCTTCTA CTGAC CTGAAGA
V20 V20 CCTGGGAGCTTGGCAGGCTCCGGGCTTGGTGCTGTCGTCTCTCAACAT AGTGA CAGCAGC
CCGAGCTGGGTTATCTGTAAGAGTGGAACCTCTGTGAAGATCGAGTGC CCAGT TTCTACA
CGTTCCCTGGACTTTCAGGCCACAACTATGTTTTGGTATCGTCAGTTC GCCCA TCTGCAG
CCGAAACAGAGTCTCATGCTGATGGCAACTTCCAATGAGGGCTCCAAG TC TGCTAGA
GCCACATACGAGCAAGGCGTCGAGAAGGACAAGTTTCTCATCAACCAT GA
GCAAGCCTGACCTTGTCCACT
hTRB hTRB 226 ATGTGCCTCAGACTTCTCTGCTGTGTGGCCATTTCTTTCTGGGGAGCC GAGAT GGGACAC
V21 V21 AGGCTCCACGGACACCAAGGTCACCCAGAGACCTAGACTTCTGGTCAA CCAGT AGCACTG
AGCAAGTGAACAGAAAGCAAAGATGGATTGTGTTCCTATAAAAGCACA CCACG TATTTCT
TAGTTATGTTTACTGGTATCGTAAGAAGCTGGAAGAAGAGCTCAAGTT GAGTC GTGCCAG
TTTGGTTTACTTTCAGAATGAAGAACTTATTCAGAAAGCAGAAATAAT AG CAGCAAA
CAATGAGCGATTTTTAGCCCAATGCTCCAAAAACTCATCCTGTACCTT GC
G
hTRB hTRB 227 ATGGGGAGCTGGGTCCTCTGCTATGTGACCCTGTGTCTCCTGGGAGCA GTGAA AACAGCT
V22 V22 GGACCCTTGGATGCTGACATCTATCAGATGCCATTCCAGCTCACTGGG GTTGG TTGTACT
GCTGGATGGGATGTGACTCTGGAGTGGAAACGGAATTTGAGACACAAT CCCAC TCTGTCC
GACATGTACTGCTACTGGTACTGGCAGGACCCAAAGCAAAATCTGAGA ACCAG TGGGAGC
CTGATCTATTACTCAAGGGTTGAAAAGGATATTCAGAGAGGAGATCTA CCA GCAC
ACTGAAGGCTACGTGTCTGCCAAGAGGAGAAGGGGCTATTTCTTCTCA
GG
hTRB hTRB 228 ATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCCTGGCAGCA CCTGG CCGGGAG
V23 V23 GACTCTTTTCATGCCAAAGTCACACAGACTCCAGGACATTTGGTCAAA CAATC ACACGGC
GGAAAAGGACAGAAAACAAAGATGGATTGTACCCCCGAAAAAGGACAT CTGTC ACTGTAT
ACTTTTGTTTATTGGTATCAACAGAATCAGAATAAAGAGTTTATGCTT CTCAG CTCTGCG
TTGATTTCCTTTCAGAATGAACAAGTTCTTCAAGAAACGGAGATGCAC AA CCAGCAG
AAGAAGCGATTCTCATCTCAATGCCCCAAGAACGCACCCTGCAG TCAATC
hTRB hTRB 229 ATGGCCTCCCTGCTCTTCTTCTGTGGGGCCTTTTATCTCCTGGGAACA GAGTC CAGCTCT
V24 V24 GGGTCCATGGATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACA TGCCA TTACTTC
AAGACAGGAAAGAGGATTATGCTGGAATGTTCTCAGACTAAGGGTCAT TCCCC TGTGCCA
GATAGAATGTACTGGTATCGACAAGACCCAGGACTGGGCCTACGGTTG AACCA CCAGTGA
ATCTATTACTCCTTTGATGTCAAAGATATAAACAAAGGAGAGATCTCT GA TTTG
GATGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCC
CTA
hTRB hTRB 230 ATGACTATCAGGCTCCTCTGCTACATGGGCTTTTATTTTCTGGGGGCA GGAGT TACCTCT
V25 V25 GGCCTCATGGAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATA CTGCC CAGTACC
GGGACAGGAAAGAAGATCACTCTGGAATGTTCTCAAACCATGGGCCAT AGGCC TCTGTGC
GACAAAATGTACTGGTATCAACAAGATCCAGGAATGGAACTACACCTC CTCAC CAGCAGT
ATCCACTATTCCTATGGAGTTAATTCCACAGAGAAGGGAGATCTTTCC A GAATA
TCTGAGTCAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACC
CT
hTRB hTRB 231 ATGAGCAACAGGCTTCTCTGCTGTGTGATCATTTGTCTCCTAAGAGCA GAAGT ACATCTG
V26 V26 GGCCTCAAGGATGCTGTAGTTACACAATTCCCAAGACACAGAATCATT CTGCC TGTATCT
GGGACAGGAAAGGAATTCATTCTACAGTGTTCCCAGAATATGAATCAT AGCAC CTATGCC
GTTACAATGTACTGGTATCGACAGGACCCAGGACTTGGACTGAAGCTG CAACC AGCAGTT
GTCTATTATTCACCTGGCACTGGGAGCACTGAAAAAGGAGATATCTCT AG CATC
GAGGGGTATCATGTTTCTTGAAATACTATAGCATCTTTTCCCCTGACC
CT
hTRB hTRB 232 ATGGGCCCCCAGCTCCTTGGCTATGTGGTCCTTTGCCTTCTAGGAGCA GGAGT ACCTCTC
V27 V27 GGCCCCCTGGAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACA CGCCC TGTACTT
GTGACTGGAAAGAAGTTAACAGTGACTTGTTCTCAGAATATGAACCAT AGCCC CTGTGCC
GAGTATATGTCCTGGTATCGACAAGACCCAGGGCTGGGCTTAAGGCAG CAACC AGCAGTT
ATCTACTATTCAATGAATGTTGAGGTGACTGATAAGGGAGATGTTCCT AG TATC
GAAGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATC
CT
hTRB hTRB 233 ATGGGAATCAGGCTCCTGTGTCGTGTGGCCTTTTGTTTCCTGGCTGTA GGAGT ACATCTA
V28 V28 GGCCTCGTAGATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAA CCGCC TGTACCT
AGGACGGGAGAGAAAGTTTTTCTGGAATGTGTCCAGGATATGGACCAT AGCAC CTGTGCC
GAAAATATGTTCTGGTATCGACAAGACCCAGGTCTGGGGCTACGGCTG CAACC AGCAGTT
ATCTATTTCTCATATGATGTTAAAATGAAAGAAAAAGGAGATATTCCT AG TATG
GAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT
CT
hTRB hTRB 234 ATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTCTGTGTTCAGT GTGAG CAGCAGC
V29 V29 GCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAACC CAACA ATATATC
TCCCTGACGATCCAGTGTCAAGTCGATAGCCAAGTCACCATGATGTTC TGAGC TCTGCAG
TGGTACCGTCAGCAACCTGGACAGAGCCTGACACTGATCGCAACTGCA CCTGA CGTTGAA
AATCAGGGCTCTGAGGCCACATATGAGAGTGGATTTGTCATTGACAAG AGA GA
TTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACT
hTRB hTRB 235 ATGCTCTGCTCTCTCCTTGCCCTTCTCCTGGGCACTTTCTTTGGGGTC GAGTT GTGACTC
V30 V30 AGATCTCAGACTATTCATCAATGGCCAGCGACCCTGGTGCAGCCTGTG CTAAG TGGCTTC
GGCAGCCCGCTCTCTCTGGAGTGCACTGTGGAGGGAACATCAAACCCC AAGCT TATCTCT
AACCTATACTGGTACCGACAGGCTGCAGGCAGGGGCCTCCAGCTGCTC CCTTC GTGCCTG
TTCTACTCCGTTGGTATTGGCCAGATCAGCTCTGAGGTGCCCCAGAAT TCA GAGTGT
CTCTCAGCCTCCAGACCCCAGGACCGGCAGTTCATCCT
