Lipodepsipeptides as antifungal and fungicidal agents
Lipodepsipeptides from Pseudomonas syringae pv. syringae were evaluated for antifungal activity. Specifically, the in vitro antifungal and fungicidal activities of three cyclic lipodepsinonapeptides syringomycin E, syringotoxin B, and syringostatin A against medically important isolates were evaluated using a standard broth microdilution susceptibility method. Erythrocyte toxicity was also evaluated. All three compounds showed broad antifungal activity and fungicidal action against most of the fungi tested.In addition, the present invention relates to a novel method for suppressing the immune system of mammals using cyclic lipodepsipeptides. The ability of cyclic lipodepsipeptides to suppress the immune system was evaluated in mitogen-induced lymphocytes. One cyclic lipodepsipeptide, syringomycin-E significantly inhibited mitogen--induced lymphocytes stimulation by pokeweed mitogen, phytohemagglutinin, and monoclonal antibodies to CD3. These results indicate that lipodepsipeptides are useful at suppressing both cellular and humoral immune responses.
Latest Utah State University Patents:
- Red mud compositions and methods related thereto
- Stable, non-corrosive electrolyte with high performance for rechargeable ion batteries
- METHOD OF MANUFACTURING CARBON FIBER-REINFORCED COMPOSITE INCLUDING THERMAL BARRIER COATING LAYER
- CLIMBING CAMS AND ATTACHMENT SYSTEMS
- Climbing cams and attachment systems
Claims
1. A method for combating plant and mammalian pathogenic fungi comprising contacting the fungi with a cyclic lipodepsipeptide said lipodepsipeptide selected from the group consisting of syringomycin, syringostatin and syringotoxin.
2. The method for combating pathogenic fungi of claim 1 wherein the lipodepsipeptide is isolated from a plant bacterium Psuedomonas stringae pv. syringae.
3. The method for combating pathogenic fungi of claim 1 wherein the lipodepsipeptide is syringomycin-E.
4. The method for combating pathogenic fungi of claim 1 wherein the lipodepsipeptide is syringostatin-B.
5. The method for combating pathogenic fungi of claim 1 wherein the lipodepsipeptide is syringostatin-A.
6. A method for combating plant and mammalian pathogenic fungi comprising contacting the fungi with a compound comprising a lipodepsipeptide having a formula: ##STR2## wherein R.sub.1 is an amino acid or a modified amino acid; and
- R.sub.2 is
7. The method for combating pathogenic pathogens of claim 6 wherein R.sub.1 is selected from the group consisting of Ser-Dab-Dab-Arg-Phe, Dab-Dab-HomoSer-Orn-Thr and Dab-Gly-Homoser-Orn-Thr.
8. The method for combating pathogenic pathogens of claim 6 wherein R.sub.2 is CH.sub.3 (CH.sub.2).sub.9 --CH(OH).
9. A method for combating pathogenic pathogens of claim 6 wherein R1 is Dab-Gly-HomoSer-Orn-Thr.
10. A method for combating pathogenic pathogens of claim 6 wherein R1 is Ser-Dab-Dab-Arg-Phe.
11. A method for combating pathogenic pathogens of claim 8 wherein R1 is Dab-Dab-HomoSer-Orn-Thr.
| 5576298 | November 19, 1996 | Strobel et al. |
- "Properties of Voltage-Gated Ion Channels Formed by Syringomycin E in Planar Lipid Bilayers", Feigin et al., The Journal of Membrane Biology, 1996, pp. 41-47. "Bacterial Phytotoxin Syringomycin and its Interactions with Host Membranes", Takemoto, Molecular Signals in Plant-Microbe Communications, 1991, pp. 247-260. Harrison, et al, J. Gen Microbiology., (1991) vol. 137, 12, (2857-2865). Sorensen et al Antimicrobial Agents and Chemothearpy 40(12) 2710-2713, 1996. Grgurina et al, Experientia 50/2; pp. 130-133, 1994.
Type: Grant
Filed: Sep 12, 1996
Date of Patent: Nov 3, 1998
Assignee: Utah State University (Logan, UT)
Inventor: Jon Y. Takemoto (North Logan, UT)
Primary Examiner: Cecilia J. Tsang
Assistant Examiner: S. G. Marshall
Law Firm: Madson & Metcalf
Application Number: 8/713,996
International Classification: A61K 3812; C07K 500; C07K 700; C07K 1600;
