Pineapple plant named ‘FR28203’
A new and distinct pineapple plant named ‘FR28203’, comprising certain unique traits, standing out Fusarium resistance obtained after crossing and backcrossing Fusarium resistant pineapple lines with an MD-2 pineapple. The invention incorporates stable, inheritable resistances to Fusariosis disease in a pineapple variety capable of addressing all of the high-quality requirements of global export markets.
Genus and species: Ananas comosus.
Variety denomination: ‘FR28203’.
This application includes material that is subject to copyright protection. The copyright owner has no objection to the facsimile reproduction by anyone of the patent disclosure, as it appears in the Patent and Trademark Office files or records, but otherwise reserves all copyright rights whatsoever.
Inventors: Hans Sauter, Joao Rodrigues and Ebrahim Firoozabady.
BACKGROUND OF THE NEW PLANTThe present invention comprises a new and distinct pineapple variety of Ananas comosus, hereinafter referred to as the variety named ‘FR28203’. The said variety has been developed by sexual crossing between plants of Manzana variety 1/2.1696 (not patented) as the male parent and the variety ‘MD-2’ (non-patented) as the female parent, and selected from large populations of progenies of hybrids (F1), backcrossed three times (BC1, BC2 and BC3). This process started in 2009 in Limoeiro do Norte, Ceará, Brazil, and took thirteen consecutive years of iterative hybridization, selection, and planting. The new variety ‘FR28203’ in particular was the result of the back-cross of a selected plant of the ‘1/2.1696’ line, with a selected plant of ‘MD-2’, to achieve the combination of characters of interest that qualify ‘FR28203’ as a variety of commercial potential.
The main objective of the hybridization program was to introduce stable resistance to Fusariosis disease of pineapples into a variety that would produce attractive fruits with excellent organoleptic traits and the right shape and size that would satisfy the strict requirements of export markets. The new cultivar ‘FR28203’ is being reproduced asexually through the tissue culture technique faithful to the type in Limoeiro do Norte, Ceará, Brazil.
BACKGROUND INFORMATION ABOUT THE DISEASEAmong all the diseases of pineapple (Ananas comosus var. comosus), Fusariose caused by Fusarium guttiforme stands out for being the one that brings largest economic losses to producers, with estimated losses between 50% and 100% of fruits and up to 50% in propagules (Matos, 2008).
Fusarium guttiforme can infect all parts of the pineapple causing the exudation of a gummy substance from the affected tissues. In plants, the lesion is located on the stem and progresses to the base of the leaf being restricted to the achlorophyllous region. Under field conditions an infected pineapple plant may show one or more external symptoms: stem tilt to the side where the lesion is located; changes in plant phyllotaxis, increasing the number of leaves per spiral; changes in the architecture of the plant that has the appearance of a funnel or cup; reduction in leaf length as well as in the overall development of the plant; “opening” of the central rosette of plants showing the youngest leaves; reducing the stem development; chlorosis; death of the apical meristem. As the disease progresses, the plant paralyzes growth and dies (Matos & Junghans, 2006).
In the fruits, the symptoms are manifested in the form of soft rot in the pulp, with accumulation of gum in the ovules of the ovary and exudation of the same through the infected floral cavity. At a more advanced stage of disease development, the skin of the fruit in the infected areas presents a red brown coloration and are at the lower level. These symptoms are observed with greater intensity in fruits near harvest (Matos & Junghans, 2006).
Following extensive field and laboratory research, four isolates of Fusarium guttiforme were identified for their particularly high virulence in the North East of Brazil, subsequently isolated and reproduced to serve as standard inoculum for the resistance trials the pineapple hybrids participating in this project were subjected to.
SUMMARY OF THE INVENTIONThe invention is related to a new and distinct pineapple variety of the Bromeliaceae family, which was derived from a backcross (BC3) between the ‘MD-2’ and the ‘1/2.1696’ (which in turn was already a backcross of a backcross between ‘MD-2’ X ‘Manzana’ cultivar), through hand pollination and mass selection process during thirteen years and provides Fusarium resistance to pineapple plants that could be grown in all areas, including where the disease caused by Fusarium guttiforme occurs.
In most characteristics, the selected hybrid is more alike to the ‘MD-2’ parent than the ‘1/2.1696’ parent. The main difference is in the resistance to Fusarium guttiforme exhibited by the ‘FR28203’ and absent in the ‘MD2’ variety. The selected backcross 3 (BC3) plant's fruit has an internal quality and pulp color like the ‘MD-2’ fruit: the total soluble solid contents (TSS), citric acid, ascorbic acid, yellow pulp color, and the size of the fruit are like the ‘MD-2’. In addition, the ‘FR28203’ fruit is cylindrical in shape, and has a very pleasant aroma, and delectable taste, just like the ‘MD-2’ variety.
Applicant has previously filed for patent for FR4139, another Fusarium resistant variety product of this program, however, in contrast to that said variety, ‘FR28203’ exhibits consistently wider peduncle diameter, this being on the average 3.3 cm wide or close to 20% wider than other varieties.
The new variety is characterized by occasional presence of spines at the leaf tip. Leaf color is gray-green and similar to the color of both parental lines, ‘MD-2’, and the BC2 ‘Manzana’ hybrid ‘1/2.1696’ (
The accompanying pictures illustrate the overall appearance of the new Ananas comosus var. comosus ‘FR28203’ showing infection damage caused by artificial inoculation with four different isolates of Fusarium guttiforme showing the colors as reasonably possible with colored reproductions of this type. The lesion caused by direct inoculation with an infected needle on the fruit helped verify the resistance trait (earlier established by immersion of young plants in a suspension of Fusarium guttiforme's most virulent isolate), when comparing reaction of fruit pulp tissue of the new pineapple variety ‘FR28203’ with ‘MD2’:
The new Ananas comosus cultivar ‘FR28203’ has not been observed under all possible environmental conditions. However, the plants were grown under environmental conditions and cultural practices which approximate those generally used in commercial pineapple growing operations. The phenotype of the new cultivar may still vary depending on the environmental conditions such as temperature, humidity, light intensity, and photoperiod without any change made to the genotype of the plant.
The aforementioned photographs, together with the following observations, measurements and values describing plants of ‘FR28203’ variety are based on observations made under optimally fertilized and growing conditions, in the region of Limoeiro do Norte, Ceará, Brazil (latitude −5.21945833° and longitude −37.914505°), where the temperatures generally range from 15.96-38.70° C., and an annual rainfall average of 746 mm.
The color terminology and designation reported here are in accordance to the Munsell Color Notation for Plants Tissues published by Munsell Color Macbeth, a division of Kollmorgen Corporation, Baltimore, Md. USA.
The following description was made based on a population of ‘FR28203’ plants, the BC3 hybrid obtained through hand pollination of parental lines in 2016 and planted in 2018 and fruit harvested in 2020 and new propagules produced from this plant planted in 2020.
- Plant identification: Name: Ananas comosus var. comosus ‘FR28203’.
- Parental lines: Selected plant (BC2) ‘1/2.1696’ backcrossed to ‘MD-2’.
- Origin: Conventional genetic improvement (hand pollination), through crossing and backcrossing.
- Classification: Botanical: Bromeliaceae or pineapple family. Subfamily: Bromelioidae. Genus: Ananas. Subgenus: comosus. Variety: ‘FR28203’.
- Commercial: Bromeliad fruit plant (pineapple).
- Form: Terrestrial (in plantation), with overlapping, sessile leaves from a funnel-formed rosette, surrounding a composite inflorescence (during anthesis), with few or no slips in the fruit peduncle and suckers that are produced in the stem and originate subsequent crops.
- General description: ‘FR28203’ (before anthesis).
- Growth habit: Semi-erect.
- Stem:
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- I. General.—Short, vertical, and covered by overlapping leaves, each leaf with a dominant axillary bud.
- II. Stem texture.—Glabrous and smooth.
- III. Stem size.—A) Length (above soil level): usually between 12.7-24.6 cm at anthesis. B) Diameter between 5.3-13.2 cm at ground level to the anthesis.
- IV. Stem shape.—Cylindrical and with a narrower diameter at the distal part.
- V. Stem color.—Cream with some tendency to brown (10YR 8/8 in the Munsell color chart).
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- Leaves:
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- I. General.—Closely overlapping sessile leaves (formed in acropetal succession) forming a dense rosette, the outline of which in longitudinal section is roughly heart shaped. The number of leaves fluctuates between 29-49 with a 5/13 phyllotaxy.
- II. Texture.—A) Upper epidermal area: Glabrous, semirigid and channeled (or concave) except at the leaf tip. B) Lower epidermal area: Finely striated (longitudinally) and appears covered with a white layer consisting of scale like trichomes.
- III. Leaf arrangement.—Alternate and in rosette shape.
- IV. Leaf margins.—No leaf piping observed. Entire margin, with rarely found irregularly spaced small deltoid cuspidate hooked spines usually located on the distal portions of leaves. Size of spines 1.36 mm
- V. Leaf venation.—Parallel.
- VI. Leaf shape.—Leaves are not uniform in shape and vary with the position of the leaf on the stem. The basal or oldest leaves are lanceolate while the base is considerably expanded. There is a noticeable narrowing in width between achlorophyllous (basal) and chlorophyllous (or main portion) of the leaves. The longest or most mature leaves are lanceolate in shape, but the base is without the arcuate expansions of the preceding leaves. The remaining leaves (or center leaves of the plant rosette) are lanceolate in form with no expansion of width into the base. The leaf apex is accuminate.
- VII. Leaf size (to anthesis).—A) Length: Usually between 99.0-106.0 cm for those ‘D’ leaves with a non chlorophyllous base that usually is between 8.0-13.4 cm in length. B) Width: Normally between 3.0-7.6 cm in the mid leaf area of the longest leaves. The expanded basal disk usually has a maximum width of 4.8-9.6 cm. C) Thickness: In the longest leaves, it usually varies between 1.0-2.5 mm at the center of the mid leaf area and decrease laterally between 0.90-1.50 mm at the margin, while becoming slightly thinner towards the tip. The expanded basal disk at the mid stem area usually has a maximum thickness of 1.87-2.75 mm at the center of the blade and tapering laterally toward margins up to 0.23-0.75 mm.
- VIII. Leaf color similar to that of ‘MD2’, mostly lacking anthocyanin.—A) Upper epidermal surface: 1. General: dominant color is usually dark green. The color of the basal disk is predominantly white and light green; achlorophyllous basal disk area is commonly pale white. Mid leaf area: commonly dark green (7.5GY 4/6 in the Munsell color chart). Leaf tip area: commonly dark green (7.5GY 4/6 in the Munsell color chart). B) Lower epidermal surface (underside): General: commonly green to pale green (N9 in the Munsell color chart) with pale white basal disk area (N8 in the Munsell color chart).
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- Inflorescence (at anthesis):
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- I. General.—Flower composite from 35-84 fruitlets borne per inflorescence of a long peduncle of approximately 13.4-19.0 cm length at the apical meristem. Individual bisexual flowers that consist of three Sepals, Six Stamens, three Stigmas and three Carpels. The inflorescence is self-incompatible producing edible fruit parthenocarpically (production of fruit without fertilization of ovules).
- II. Texture.—Glabrous and fibrous.
- III. Shape.—Oval with slightly raised flowers with a light red to grayish red color in the crown.
- IV. Size and color.—Comparable to specimens of Ananas comosus var. comosus. Petal size: 1.40 cm. Petal color in the apex: light purple (10P 6/12 in the Munsell color chart).
- V. Sepal size.—0.67 cm. Sepal color: reddish brown (5RP 4/4 in the Munsell color chart).
- VI Floral bract's length.—From 1.7 cm, serrated margins (with tiny spines); yellowish brown color (7.5Y 4/4 in the Munsell color chart).
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- Crown (at harvest):
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- I. General.—Visually one crown. composed on average of 96 leaves. Crown leaves are short, lanceolate in shape, and erect at anthesis, measuring on average 6.8 cm.
- II. Leaf arrangement.—Alternate and in rosette shape.
- III Leaf margins.—Entire with smooth borders. Seldom very small spines in the tip of one leaf.
- IV. Crown size at harvest.—Average 14.2 cm. Weight: average 172 g.
- V. Shape.—Medium crown with medium width and semirigid leaves, with the crown normally not exceeding length nor width of fruit and with semirigid leaves.
- VI. Attitude.—Upright.
- VII. Color of the terminal crown leaves.—A. upper surface: Dark green color at the apex (5GY 1/2) and dark green at the base (5GY 1/2). B. Lower surface: grayish green (10GY 6/5 in the Munsell color chart).
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- Fruit (at harvest):
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- I. Size.—Usually with a weight between 851-2486 grams and average weight of 1954 grams. Fruit core's diameter 2.1 cm. Fruit core's color: yellow to dark yellow (7.5Y 9/6 in the Munsell color chart).
- II. Shape.—Cylindrical with flat and medium size fruitlets. Medium crown with thin and semirigid leaves, wherein the crown normally is not exceeding length nor width of fruit. Average fruit's height 16.7 cm, fruit's diameter: average 11.0 cm.
- III. How borne.—The development of the fruit occurs from the apical meristem of the plant on a long and thick peduncle, usually between 13.4-19.0 cm in length and 3.28 cm in width. No slips available for evaluation. 6 long peduncle bracts, with spines on the edges and of medium green to dark green color (7.5 GY 7/8 in the Munsell color chart) are generally present at the base of the fruit.
- IV. Color.—A) Shell: commonly grayish green at early maturity (5GY 1/2), with yellowish brown bract (7.5Y 4/4). Fruit with yellow peduncle (2.5Y 5/6). B) Pulp: Usually yellow (2.5Y 5/6).
- V. Fruit flesh.—Dense, firm, medium in fiber and juiciness, emitting characteristic pineapple aroma; flesh color distinctly yellow (7.5Y 8/6 in the Munsell color chart).
- VI. Brix.—Typically, average 17.4 degrees, standing out from their parents.
- VII. Total acid levels.—Usually between 0.35-0.74 milligrams of citric acid/ml of juice, with an average of 0.48 mg/ml.
- VIII. Vitamin C content reported as ascorbic acid content. Regularly between 34.41-51.40 mg/100 ml of juice, with an average of 43.42 mg/100 ml.
- IX. Plant/fruit resistance/susceptibility to pest and diseases. The plant of the new variety ‘FR28203’ performs very similar to ‘MD-2’ differentiating mainly in its resistance to Fusarium guttiforme. Table 2 shows the comparison of pineapple varieties ‘FR28203’, ‘MD-2’ and ‘Champaka’ (not patented) and their resistance to Fusarium guttiforme.
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- Others:
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- I. Fertility.—This plant is self-incompatible. This is the reason why the presence of sexual seeds is almost null. The material used for planting are suckers, fruit crowns and vitro plants.
- II. Vigor.—This plant exhibits similar vigor as its parents, the ‘1/2.1696’ line, and the ‘MD-2’ variety.
- III. Yield.—A population of this pineapple can have an agronomic yield from 82 to 152 ton/ha.
- IV. Plant use.—The fruit will be commercialized within the fresh fruit and processed fruit for the export markets. Shipping conditions are typically set as 14 days at 7 degrees Celsius.
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- Summary of the special characteristics: The ‘FR28203’ plants present the following differences when compared to its parental lines:
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- A. Resistance to Fusarium guttiforme.—MD-2 is very susceptible to this pathogen, while ‘FR28203’ exhibits total resistance.
- B. Yellow colored pulp.—The backcross between the ‘1/2.1696’ backcross and ‘MD-2’, ended up in a combination of a fruit with similar shell color and yellow pulp like the ‘MD-2’ variety.
- C. Plant with little or none slips.—‘MD-2’ variety produces between 1-3 slips per plant, and the ‘1/2.1696’ produces little or none slips per plant. By comparison, ‘FR28203’, which reduces cosmetic and pest problems that can be originated by the contact of the slips with the fruit.
- D. At full maturity.—The ‘FR28203’ fruit achieves high sugar content, as measured by brix levels. Citric acid, and ascorbic acid are similar as those of ‘MD-2’ and higher compared to ‘1/2.1696’ fruits.
- E. As a result of the backcross process.—‘FR28203’ has acquired resistance to Fusarium guttiforme, just like the ‘1/2.1696’ but with an improved fruit size, pulp color, and internal quality similar to the ‘MD-2’. This combination of characteristics is important to differentiate this hybrid from its parents; as shown on Table 3, when reproduced asexually, these characteristics are stable and reproducible for successive generations.
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- Individual plant description: The following is an overview of ‘FR28203’ hybrid, a new pineapple plant variety, that was developed through hybridization process in Limoeiro do Norte, Ceará, Brazil.
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- Plant age.—15.97 months after planting plus 4.99 months after forcing.
- Plant growth habit.—Semi-upright.
- Plant diameter.—Around 165 cm between opposite leaf tips.
- Plant height.—129 cm above ground surface.
- Stem.—Length. 19.0 cm above ground sur-face. Diameter. Between 6.1 cm above ground surface.
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- Leaves:
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- I. Number.—59 leaves.
- II. Length.—106 cm the longest leaf.
- III. Width.—(Largest leaves) at mid leaf (max.) 5.8 cm. Leaf piping is absent.
- IV. Thickness.—2.3 mm along the axis.
- V. Color.—A) Upper epidermal chlorophyllous area: Commonly dark green (7.5GY 4/6). B) Upper epidermal non chlorophyllous area: commonly pale white (N8). C) Lower epidermal area: Commonly pale green (N8). Leaf anthocyanin coloration may appear slightly on some plants.
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- Inflorescence:
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- General.—Composite inflorescence borne on a long peduncle at the apical meristem. The inflorescence is composed of 84 flowers, which eventually lead to an equal number of fruitlets.
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The pineapple variety ‘FR28203’ has a post-harvest shelf life similar to ‘MD-2’ variety, showing similar performance in shell dehydration studies.
‘FR28203’ fruit general characteristics are as follows (A) a fruitlet apex which is flat; (B) the flesh density is medium; (C) the number fruitlets per syncarp is 47-93; (D) the fruitlet characteristics are as follows:
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- III. Shell color.—‘FR28203’ shell color at mature (10YR 5/8) and immature (5GY 1/2) stages.
- IV. Weight and shape of fruit.—‘FR28203’ average fruit weight is similar to the ‘MD-2’, and ‘Champaka’; ‘FR28203’ has a cylindrical shape.
- V. Ascorbic acid.—‘FR28203’ has a higher content of ascorbic acid than its relative A. comosus cv. ‘Manzana’, but similar to ‘MD-2’ variety.
- VI. Citric acid.—‘FR28203’ citric acid content is similar to ‘MD-2’.
- VII. Brix.—The sugar content (measured as brix degrees) of ‘FR28203’ is higher to that found on ‘MD-2’.
- VIII. Age to forcing.—‘FR28203’ is vigorous and can reach forcing plant size 8-17 months after planting (shorter when using vegetative propagation material, and longer when starting with seed). Although plant development time to forcing depends on the size of planting material used and the desired plant weight at floral induction, the above figures are similar to those achieved by the ‘MD-2’ variety, which reaches an optimal forcing size in 8.5 months after planting when using vegetative propagation material.
- IX. Leaf spines.—This characteristic is commonly used to differentiate pineapple plants from other varieties. The color of the leaf spines are as follows: Tip: dark green like MD2 (7.5GY 4/6), base: dark green like MD2 (7.5GY 4/6). ‘FR28203’ does not have conspicuous or regular thorns on the leaf like its parental ‘1/2.1696’, or its parental ‘MD-2’, although ‘MD-2’ often presents irregular thorns on the edges of the leaf blade as well.
- X. Fruit quality of ‘FR28203’ when compared with other pineapple varieties.—Most pineapple varieties grown worldwide are produced for local consumption, and very few are grown for international commercialization and global distribution. The fruit characteristics bred into ‘FR28203’ were specifically chosen to comply with the strictest quality standards of export markets like those of North America, Europe, and Asia Pacific. As shown in Table 5, ‘FR11834’ compares very favorably against ‘MD-2’, the pineapple variety that has achieved most notoriety to date in global markets.
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- MATOS, A. P. (2008)—Perdas causadas pela F. guttiforme. Disponivel em: https://www.embrapa.br/busca-de-publicacoes/-/publicacao/981520/situacao-e-perspectivas-da-abacaxicultura-no-amazonas
- MATOS, A. P. & JUNEGHANS, D. T. Variedades de abacaxi resistentes a F. guttiforme. 2006. Cruz das Almas, B A. https://ainfo.cnptia.embrapa.br/digital/bitstream/item/42932/1/VARIEDADES-ABACAXI-ARISTOTELES.pdf.
- Munsell Color chart for Plant Tissues. published by Munsell Color Macbeth, a division of Kollmorgen Corporation, Baltimore, Md. USA.
Claims
1. A new and distinct Ananas comosus plant named ‘FR28203’, as illustrated and described herein.
Type: Grant
Filed: Sep 24, 2024
Date of Patent: Jul 1, 2025
Assignee: Del Monte International GMBH (Zug)
Inventors: Hans Sauter (Coral Gables, FL), Joao Rodrigues (Ceará), Ebrahim Firoozabady (Pleasant Hill, CA)
Primary Examiner: Susan McCormick Ewoldt
Application Number: 18/895,042
International Classification: A01H 5/08 (20180101); A01H 6/22 (20180101);