Patents Issued in March 6, 2003
  • Publication number: 20030044814
    Abstract: The present invention provides nucleic acid and protein sequences for a novel protein, PRC17, and methods, reagents, and kits for diagnosing and treating cancer in a mammal, e.g., a human. This invention is based upon the discovery that the novel PRC17 is overexpressed and/or amplified in cancer. Methods to detect cancer or a propensity to develop cancer, to monitor the efficacy of a cancer treatment, and to treat cancer, by inhibiting the expression and/or activity of PRC17 in a cancer cell are included.
    Type: Application
    Filed: February 8, 2002
    Publication date: March 6, 2003
    Applicant: Tularik Inc.
    Inventors: Jing Li, Scott Powers, Phil Xiang, Yue Peng
  • Publication number: 20030044815
    Abstract: The present invention relates to newly identified nucleic acids and polypeptides present in normal and neoplastic breast cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of the polypeptides of the invention. The invention also relates to compositions comprising the nucleic acids, polypeptides, antibodies, variants, derivatives, agonists and antagonists of the invention and methods for the use of these compositions. These uses include identifying, diagnosing, monitoring, staging, imaging and treating breast cancer and non-cancerous disease states in breast tissue, identifying breast tissue, monitoring and identifying and/or designing agonists and antagonists of polypeptides of the invention. The uses also include gene therapy, production of transgenic animals and cells, and production of engineered breast tissue for treatment and research.
    Type: Application
    Filed: February 14, 2002
    Publication date: March 6, 2003
    Inventors: Susana Salceda, Roberto Macina, Ping Hu, Herve E. Recipon, Kalpana Karra, Robert Cafferkey, Yongming Sun, Chenghua Liu
  • Publication number: 20030044816
    Abstract: The invention relates to a method for detecting a double-stranded region in a nucleic acid by (1) providing two separate, adjacent pools of a medium and a interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage of a single-stranded nucleic acid, but not of a double-stranded nucleic acid, from one pool to the other pool; (2) placing a nucleic acid polymer in one of the two pools; and (3) taking measurements as each of the nucleotide monomers of the single-stranded nucleic acid polymer passes through the channel so as to differentiate between nucleotide monomers that are hybridized to another nucleotide monomer before entering the channel and nucleotide monomers that are not hybridized to another nucleotide monomer before entering the channel.
    Type: Application
    Filed: February 20, 2002
    Publication date: March 6, 2003
    Inventors: Timothy J. Denison, Alexis Sauer, Jene Golovchenko, Amit Meller, Eric Brandin, Daniel Branton
  • Publication number: 20030044817
    Abstract: The present invention provides modified primers for use in the amplification of a nucleic acid sequence. Amplifications carried out using the modified primers result in less template-independent non-specific product (primer dimer) compared to amplifications carried out using unmodified primers.
    Type: Application
    Filed: October 24, 2001
    Publication date: March 6, 2003
    Inventors: Walter J. Laird, John T. Niemiec
  • Publication number: 20030044818
    Abstract: This invention is directed towards a method for obtaining nucleic acid ligands against target proteins without directly purifying the target proteins. The method used in the invention is called SELEX, which is an acronym for Systematic Evolution of Ligands by EXponential enrichment. The nucleic acid ligands of the invention are useful as diagnostic and therapeutic agents for diseases in which the targets proteins play a causative role.
    Type: Application
    Filed: March 12, 2002
    Publication date: March 6, 2003
    Inventors: Larry Gold, Dominic A. Zichi, Jonathan Drew Smith
  • Publication number: 20030044819
    Abstract: The present invention provides methods of preparing a triple-stranded DNA molecule by forming a DNA and protein complex, which DNA has a linear double-strand molecule and a single-strand molecule where the linear single-stranded DNA is complementary to a 5′ end region of one strand in the linear double stranded DNA; and the protein component is a homogeneous recombinant protein and an exonuclease; and then the protein is removed from the complex; triple-stranded DNA; and methods of using the triple-stranded DNA to detect nucleic acid sequences.
    Type: Application
    Filed: March 21, 2002
    Publication date: March 6, 2003
    Applicant: AISIN SEIKI KABUSHIKI KAISHA
    Inventor: Yasushi Shigemori
  • Publication number: 20030044820
    Abstract: A method for cloning a nucleic acid fragment into a vector by flanking the fragment with first and second adapter sequences, and contacting the fragment with the vector having sequences homologous to the first and second adapter sequences under conditions such that the nucleic acid fragment is incorporated into the vector by homologous recombination in vivo in a host cell. Additionally, a method for selecting for a successful transformation of a vector by a nucleic acid insert. Also, systems for cloning a nucleic acid fragment into a vector without at least one of a restriction enzyme, a ligase, a gyrase, a single stranded DNA binding protein, or other DNA modifying enzymes. Further, a kit for cloning a nucleic acid fragment into a vector.
    Type: Application
    Filed: April 16, 2002
    Publication date: March 6, 2003
    Inventors: Xiaowu Liang, Andy Teng, Shizhong Chen, Dongyuan Xia, Philip L. Felgner
  • Publication number: 20030044821
    Abstract: Identifying the genetic determinants for disease and disease predisposition remains one of the outstanding goals of the human genome project. When large patient populations are available, genetic approaches using single nucleotide polymorphism markers have the potential to identify relevant genes directly. While individual genotyping is the most powerful method for establishing association, determining allele frequencies in DNA pooled on the basis of phenotypic value can also reveal association at a much-reduced cost. Here we analyze pooling methods to establish association between a genetic polymorphism and a quantitative phenotype. Exact results are provided for the statistical power for a number of pooling designs where the phenotype is described by a variance components model and the fraction of the population pooled is optimized to minimize the population requirements.
    Type: Application
    Filed: April 22, 2002
    Publication date: March 6, 2003
    Inventors: Joel S. Bader, Aruna Bansal, Pak Sham
  • Publication number: 20030044822
    Abstract: The invention is a method for genomic subtractive hybridization. Specific nucleic acid sequences are removed from a sample of nucleic acid sequences by specifically hybridizing the sequences to a complementary nucleic acid sequence bound to a target molecule such as biotin. The target molecule is then contacted with a binding partner such as avidin and separated from the sample of nucleic acid sequences. As the target is separated from the sample the hybridized nucleic acid sequences are also removed from the sample. The method preferably involves the removal of repetitive nucleic acid sequences from a nucleic acid sample to generate a library of probes that are substantially free of repetitive nucleic acid sequences.
    Type: Application
    Filed: April 26, 2002
    Publication date: March 6, 2003
    Applicant: The Brigham and Women's Hospital, Inc.
    Inventors: Jonathan Fletcher, Sheng Xiao
  • Publication number: 20030044823
    Abstract: The invention provides novel reagents, reagent kits, and methods for automated hybridization. More particularly, the invention provides reagents, reagent kits, and methods for automated in situ hybridization and automated hybridization on a microarray. The use of automated instruments for in situ hybridization and microarray hybridization dramatically reduces the amount of labor and time involved and also facilitates standardization of protocols and consistency between results.
    Type: Application
    Filed: April 30, 2002
    Publication date: March 6, 2003
    Inventors: Catherine Wolf, Hiroaki Nitta, Thomas Grogan, Jacques Cavadenti, Lidija Pestic-Dragovich, Anthony Hartman, Angela Sattler, Jennifer Wong
  • Publication number: 20030044824
    Abstract: Pseudo-positive data can be reduced by incubating a solution containing an enzyme in the absence of a test substance and a substrate of the enzyme.
    Type: Application
    Filed: June 10, 2002
    Publication date: March 6, 2003
    Inventor: Kenji Abe
  • Publication number: 20030044825
    Abstract: The present invention is to provide a novel protein and a novel gene which are related to bone metabolism, in particular, differentiation (maturation) of osteoblast or morphological change (retraction), and to disclose a polypeptide which comprises a polypeptide selected from the following (A), (B) and (C), and has a function or an activity selected from the following (i), (ii) and (iii):
    Type: Application
    Filed: May 22, 2002
    Publication date: March 6, 2003
    Inventors: Yuji Imai, Hiroyuki Akatsuka, Eri Kawai, Kenji Omori, Noriyuki Yanaka, Naoki Sakurai
  • Publication number: 20030044826
    Abstract: Disclosed are systems and methods for identifying and quantitating the presence of one or more DNA species in a sample population through PCR amplification. DNA species quantitation includes a determination of a threshold fluorescence value used in the assessment of the PCR amplification reaction. Various embodiments of the present invention incorporate an enhancement function useful in selecting appropriate threshold fluorescence values and facilitate the determination of DNA concentrations by quantitative PCR based methodologies.
    Type: Application
    Filed: May 23, 2002
    Publication date: March 6, 2003
    Inventors: Lesley Ward, Adrian Jensen, Justin Lyon, Cameron McLeman, Bryan Tysinger
  • Publication number: 20030044827
    Abstract: A method for immobilizing a DNA upon preparation of a DNA microbead array, the method comprising forming a covalent bond between a DNA to be immobilized and an anti-tag DNA on a microbead using a DNA polymerase and a DNA ligase, wherein the DNA polymerase and the DNA ligase are reacted at the same time.
    Type: Application
    Filed: May 30, 2002
    Publication date: March 6, 2003
    Applicant: Takara Bio Inc.
    Inventors: Shusaku Yamashita, Nobuto Koyama, Junichi Mineno, Ikunoshin Kato
  • Publication number: 20030044828
    Abstract: The present invention provides a method for identifying a gene which is expressed at a modulated level in an aggressive cancer by: (a) measuring baseline expression levels of genes in cancer cells; (b) introducing into the cancer cells an expression vector which directs expression of rat PEG-3; (c) measuring the expression levels of genes in the cells from step (b); and (d) identifying genes, the expression of which is modulated in an aggressive cancer. In another aspect of this invention, the measurement of expression levels of genes in step (a) and/or step (c) comprises measuring mRNA levels. In yet another aspect of the invention, the measurement of expression levels of genes in step (a) and/or step (c) comprises measuring RNA levels. In another aspect of the invention, the genes identified in step (d) are used for diagnosing whether a subject suffers from an aggressive form of cancer.
    Type: Application
    Filed: June 20, 2002
    Publication date: March 6, 2003
    Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
    Inventors: Paul B. Fisher, Zao-Zhong Su
  • Publication number: 20030044829
    Abstract: This invention provides methods for detecting or inferring the presence of malignant or premalignant cells in a human wherein the malignant or premalignant cells express COX-2. The methods of the invention detect extracellular COX-2 RNA in blood, plasma, serum, and other bodily fluids. The inventive methods are useful for aiding detection, diagnosis, monitoring, treatment, or evaluation of neoplastic disease, and for identifying individuals for whom COX-2 directed therapies would be beneficial.
    Type: Application
    Filed: June 24, 2002
    Publication date: March 6, 2003
    Inventor: Michael S. Kopreski
  • Publication number: 20030044830
    Abstract: The present invention provides a method for detecting fluorescence by using a solid support to which a probe molecule to be detected is fixed, wherein background is reduced by using a quenching agent. By using present invention, detection sensitivity of a DNA chip can be increased and stable data can be obtained.
    Type: Application
    Filed: June 26, 2002
    Publication date: March 6, 2003
    Inventors: Yoshihide Iwaki, Hiroshi Shinoki, Osamu Seshimoto, Kouki Nakamura
  • Publication number: 20030044831
    Abstract: This application describes an antibody that specifically binds to a synthetic oligomer (e.g., an oligonucleotide or oligopeptide) having a organic protecting group covalently bound thereto, which antibody does not bind to that synthetic oligomer when the organic protecting group is not covalently bound thereto. Methods of making and using such antibodies are also disclosed, along with cells for making such antibodies and articles carrying immobilized oligomers that can be used in assay procedures with such antibodies.
    Type: Application
    Filed: July 8, 2002
    Publication date: March 6, 2003
    Inventors: Paul F. Agris, Christopher D.J. Pearce, Lloyd G. Mitchell
  • Publication number: 20030044832
    Abstract: A micro flow system is provided for separating particles, comprising a microfabricated member having a flow channel (5) defined therein for guiding a flow of a fluid containing the particles through the flow channel, first inlet means (2) positioned at one end of the flow channel for entering the fluid into the flow channel, first outlet means (7) positioned at the other end of the flow channel for discharging the fluid from the flow channel, the flow of the fluid containing the particles being controlled in such a way that one particle at the time passes a cross section of the flow channel, the member being positioned in a field that is substantially perpendicular to a longitudinal axis of the flow channel so that particles residing in the flow channel and being susceptible to the field across the flow channel are deflected in the direction of the field.
    Type: Application
    Filed: July 9, 2002
    Publication date: March 6, 2003
    Applicant: SCANDINAVIAN MICRO BIODEVICES A/S
    Inventor: Gert Blankenstein
  • Publication number: 20030044833
    Abstract: Multi-analyte microarrays are produced by first attaching to the surface of a solid support, a silane with an electrophilic terminal group, contacting the terminal group with a polyamine, and activating the polyamine with an electrophilic, bifunctional linker.
    Type: Application
    Filed: September 4, 2002
    Publication date: March 6, 2003
    Applicant: Randox Laboratories Ltd.
    Inventors: Elouard Benchikh, Robert Ivan McConnell, John Victor Lamont, Stephen Peter Fitzgerald
  • Publication number: 20030044834
    Abstract: The present invention provides the nucleotide and amino acid sequence of a previously unidentified erbB receptor target. The nucleotide and amino acid sequence is set out in the figure.
    Type: Application
    Filed: September 11, 2002
    Publication date: March 6, 2003
    Inventors: Roger John Daly, Robert Lyndsay Sutherland
  • Publication number: 20030044835
    Abstract: Disclosed is a process to construct multi-component biomolecule or cellular arrays suitable for use in SPR imaging studies of large molecule, cellular/molecular, and cell/cell interactions. The success of the procedure hinges on the use of a reversible protecting group to modify reversibly &ohgr;-functionalized alkanethiols self-assembled on metal substrates.
    Type: Application
    Filed: September 30, 2002
    Publication date: March 6, 2003
    Inventors: Robert M. Corn, Anthony G. Frutos, Jennifer M. Brockman
  • Publication number: 20030044836
    Abstract: Systems and methods for enhanced quantitative analysis of hybridization intensity measurements obtained from oligonucleotide probes and other probes exposed to target samples are provided by virtue of the present invention. One embodiment ameliorates the effects of high frequency noise superimposed on a hybridization intensity measurement signal measured over successive probe alignments to a target sample sequence. Detection of expressed genes and ESTs and quantitative measurement of expression level may be improved. Mutation detection and base calling may be improved.
    Type: Application
    Filed: September 30, 2002
    Publication date: March 6, 2003
    Applicant: Princeton University, Office of Technology & Trademark Licensing
    Inventors: Arnold J. Levine, Uri Alon
  • Publication number: 20030044837
    Abstract: A method and apparatus for incubating a liquid reagent with target spots on a microarray substrate. A deformable cover is placed over the surface of the microarray substrate having the target spots with the liquid reagent between the microarray substrate and the deformable cover, and a device is used to apply a force to the deformable cover.
    Type: Application
    Filed: October 11, 2002
    Publication date: March 6, 2003
    Applicant: Packard BioScience Corporation
    Inventors: Mack J. Schermer, C. Todd Lombardo, Robert Milkowski, Donald Regan, Paul St. Cyr
  • Publication number: 20030044838
    Abstract: Peptides which bind to surfaces of bacterial spores have been identified by means of biopanning using phage-displayed peptide libraries. The sequences identified thereby are useful for binding studies to determine presence of spores in the environment or clinical setting.
    Type: Application
    Filed: January 14, 1999
    Publication date: March 6, 2003
    Inventor: CHARLES L. TURNBOUGH
  • Publication number: 20030044839
    Abstract: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.
    Type: Application
    Filed: July 10, 2001
    Publication date: March 6, 2003
    Applicant: Genentech, Inc.
    Inventors: Avi Ashkenazi, David Botstein, Luc Desnoyers, Dan L. Eaton, Napoleone Ferrara, Ellen Filvaroff, Sherman Fong, Wei-Qiang Gao, Hanspeter Gerber, Mary E. Gerritsen, Audrey Goddard, Paul J. Godowski, J. Christopher Grimaldi, Austin L. Gurney, Kenneth J. Hillan, Ivar J. Kljavin, Jennie P. Mather, James Pan, Nicholas F. Paoni, Margaret Ann Roy, Timothy A. Stewart, Daniel Tumas, P. Mickey Williams, William I. Wood
  • Publication number: 20030044840
    Abstract: The invention provides novel solid supports comprising dihydroxyalkyl aminoalkyl and dihydroxyalkylaminobenzyl groups, and methods for making and using them. The supports are particularly useful for immobilizing and derivatizing functionalized boronic acids for use in solid phase synthesis, such as those used in combinatorial chemistries. The compositions and methods of the invention are also useful as scavenger solid supports, e.g., in solution-phase parallel synthesis of small molecule libraries, and for use in resin-to-resin transfer reactions via phase transfer of solid supported boronic acids under both aqueous and anhydrous conditions. The methods of the invention provide convergent solid-phase synthesis of symmetrically or unsymmetrically functionalized compounds, such as biphenyl compounds. Also provided are synthesizer devices, e.g., semiautomated parallel synthesizers.
    Type: Application
    Filed: August 31, 2001
    Publication date: March 6, 2003
    Inventor: Dennis G. Hall
  • Publication number: 20030044841
    Abstract: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.
    Type: Application
    Filed: December 6, 2001
    Publication date: March 6, 2003
    Applicant: Genentech, Inc.
    Inventors: Kevin` P. Baker, David Botstein, Luc Desnoyers, Dan L. Eaton, Napoleone Ferrara, Sherman Fong, Wei-Qiang Gao, Audrey Goddard, Paul J. Godowski, J. Christopher Grimaldi, Austin L. Gurney, Kenneth J. Hillan, James Pan, Nicholas F. Paoni, Margaret Ann Roy, Victoria Smith, Timothy A. Stewart, Daniel Tumas, Colin K. Watanabe, P. Mickey Williams, William I. Wood
  • Publication number: 20030044842
    Abstract: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.
    Type: Application
    Filed: December 26, 2001
    Publication date: March 6, 2003
    Applicant: Genentech, Inc.
    Inventors: Luc Desnoyers, Dan L. Eaton, Audrey Goddard, Paul J. Godowski, Austin L. Gurney, James Pan, Timothy A. Stewart, Colin K. Watanabe, William I. Wood, Zemin Zhang
  • Publication number: 20030044843
    Abstract: The present invention provides a proteome analysis method including grouping a proteome into membrane proteins and compounds capable of interacting with the membrane proteins, while retaining their native structure and function, and analyzing both the membrane proteins and the compounds based on biological affinity, and devices therefor.
    Type: Application
    Filed: January 3, 2002
    Publication date: March 6, 2003
    Applicant: Mitsubishi Pharma Corporation
    Inventors: Kenji Tanaka, Lyang-ja Lee, Hiromichi Mukai, Koji Munechika, Hisashi Arikuni, Mieko Shiwa, Bungo Ochiai
  • Publication number: 20030044844
    Abstract: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.
    Type: Application
    Filed: February 1, 2002
    Publication date: March 6, 2003
    Applicant: Genentech, Inc.
    Inventors: Avi J. Ashkenazi, Kevin P. Baker, David A. Botstein, Luc Desnoyers, Dan L. Eaton, Napoleone Ferrara, Sherman Fong, Wei-Qiang Gao, Hanspeter Gerber, Mary E. Gerritsen, Audrey Goddard, Paul J. Godowski, Austin L. Gurney, Ivar J. Kljavin, Jennie P. Mather, Mary A. Napier, James Pan, Nicholas F. Paoni, Margaret Ann Roy, Timothy A. Stewart, Daniel Tumas, Colin K. Watanabe, P. Mickey Williams, William I. Wood, Zemin Zhang
  • Publication number: 20030044845
    Abstract: Novel multi-binding compounds (agents) are disclosed which bind cell membrane transporters including ion channels, molecular transporters and ion pumps. The compounds of this invention comprise from 2 to 10 ligands each of which can bind to such cellular transporters to modulate the biological processes/functions thereof. Each of the ligands is covalently attached to a linker (framework) to provide for a multi-binding compound. The linker is selected such that the multi-binding compound exhibits increased modulation of the biological processes/functions of the transporter as compared to the aggregate of the individual ligand units made available for binding to the transporter.
    Type: Application
    Filed: February 13, 2002
    Publication date: March 6, 2003
    Inventors: Thomas E. Jenkins, Burton G. Christensen, John H. Griffin, J. Kevin Judice
  • Publication number: 20030044846
    Abstract: The invention provides a chemical compound library containing a collection of chromatographic fractions purified from biological sources suitable for high-throughput screening for biological activity, wherein: (a) the chromatographic fractions contain primarily from about 1 microgram to 100 micrograms of primarily one to five chemical compounds; (b) the compounds have log P of greater than-1 and less than 5 and molecular weights less than 3,000 Daltons; (c) the library can include chemical compounds produced from at least fifty different biological sources; and (d) the library can contain at least about 200 different chemical compounds from each different biological source.
    Type: Application
    Filed: April 3, 2002
    Publication date: March 6, 2003
    Inventors: Gary Eldridge, Lu Zeng, Chris Lee, Peadar Cremin, Helene C. Vervoort, Marilyn Ghanem
  • Publication number: 20030044847
    Abstract: The present invention describes a method for detecting the interaction of at least one intracellular protein and an extracellular protein using fluorescent markers and an FRET system. The method can be used to elucidate biological pathways and to evaluate potential drug candidates of therapeutic interest.
    Type: Application
    Filed: May 15, 2002
    Publication date: March 6, 2003
    Inventors: Sidney Pestka, Christopher D. Krause
  • Publication number: 20030044848
    Abstract: The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide.
    Type: Application
    Filed: June 19, 2002
    Publication date: March 6, 2003
    Applicant: CELL SIGNALING TECHNOLOGY, INC.
    Inventors: John Rush, Hui Zhang, Xiangming Zha, Michael J. Comb, Yi Tan
  • Publication number: 20030044849
    Abstract: Methods, compounds, and polyclonal antibody libraries (PALs) that relate to screening and selection of antibodies and the cells that produce them are disclosed. In particular, an antigen or a subset of antigens which has some desired feature can be distinguished from among a multitude of other undesired antigens present in a heterogeneous antigen mixture which lack such feature by use of PALs raised against the undesired antigens. When such PALs are brought into contact with a heterogeneous antigen substrate, the antibodies in the PAL can bind to those antigens, masking them from subsequent binding by a test antibody having a similar specificity. By decreasing binding of test antibodies to those undesired antigens, if binding of test antibodies to the substrate occurs, such binding will more likely be by way of binding of the antibody to a desired antigen in the mixed antigen substrate. Thus, this invention can improve the efficiency of screening antibodies directed towards desired antigens.
    Type: Application
    Filed: August 21, 2002
    Publication date: March 6, 2003
    Inventor: Steven Kessler
  • Publication number: 20030044850
    Abstract: Synthesis particles useful for the preparation of chemical libraries and a process of using the same. The particle is of a sufficient size such that it fits into a 384 well or higher density plate and is capable of a loading capacity that allows for numerous assays to be run and then identification by mass spec or NMR of active compounds.
    Type: Application
    Filed: August 28, 2002
    Publication date: March 6, 2003
    Inventor: Andrew P. Combs
  • Publication number: 20030044851
    Abstract: The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating disorders related to these novel human secreted proteins.
    Type: Application
    Filed: December 12, 2001
    Publication date: March 6, 2003
    Applicant: Human Genome Sciences, Inc.
    Inventors: Steven M. Ruben, Jian Ni, Craig A. Rosen, Ying-Fei Wei, Paul E. Young, Kimberly A. Florence, Daniel R. Soppet, Laurie A. Brewer, Gregory A. Endress, Kenneth C. Carter, Michael Mucenski, Reinhard Ebner, David W. LaFleur, Henrik S. Olsen, Yanggu Shi, Paul A. Moore, George A. Komatsoulis
  • Publication number: 20030044852
    Abstract: The invention features a method of identifying, evaluating or making a compound or agent, e.g., a candidate compound or agent, for treatment of a disorder characterized by insulin resistance. The method includes evaluating the ability of a compound or agent to bind IKK-&bgr; or modulate IKK-&bgr; activity, to thereby identify a compound or agent for the treatment of a disorder characterized by insulin resistance. The invention also features compounds for treating insulin resistance identified by such methods, and methods of treating a subject having a disorder characterized by insulin resistance by administering such agents.
    Type: Application
    Filed: October 11, 2002
    Publication date: March 6, 2003
    Applicant: Joslin Diabetes Center, Inc., a Massachusetts corporation
    Inventor: Steven Shoelson
  • Publication number: 20030044853
    Abstract: The invention pertains to devices and methods relating to the formation of one or more fluid lanes on a substrate surface, to expose a portion of a target region on the surface to one or more reagents. Typically, a flow passage is provided that is defined at least in part by a substrate having a target region on a surface thereof. One or more inlets each allows a fluid to be introduced in contiguous laminar flow through the flow passage to form a fluid lane downstream from the inlet over a portion of the target region. Typically, at least one inlet is position to allow a fluid to be introduced directly into the flow passage in a direction nonparallel to the flow passage. Typically, the inventive devices and methods may be employed to determine the effects of a plurality of candidate compounds on a monolayer of immobilized cells.
    Type: Application
    Filed: August 28, 2001
    Publication date: March 6, 2003
    Inventors: David Socks, Thomas Ahl
  • Publication number: 20030044854
    Abstract: Test strips for determining the concentration of at least one analyte, e.g., glucose, in a physiological sample and methods for their manufacture and use and are provided. The subject test strips include a transfer element for facilitating the transfer of sample to a reaction area of the test strip. In certain embodiments, the transfer element, typically porous, has a first area and a second area, and in certain embodiments the two areas have different thicknesses. In other embodiments, the transfer element is non-porous and is configured to transfer sample by wicking it between the transfer element and the reaction area of the test strip. In the subject methods, the transport element facilitates transfers of a sample to a reaction area of the test strip. The subject test strips and methods find use in a variety of different applications, particularly in the determination of glucose concentrations.
    Type: Application
    Filed: September 5, 2001
    Publication date: March 6, 2003
    Inventors: David Matzinger, Khalid R. Quraishi
  • Publication number: 20030044855
    Abstract: Microarrays are prepared by using a separate fiber for each compound being used in the microarray. The fibers are bundled and sectioned to form a thin microarray that may be glued to a backing.
    Type: Application
    Filed: January 25, 2002
    Publication date: March 6, 2003
    Inventors: N. Leigh Anderson, Norman G. Anderson, James A. Braatz
  • Publication number: 20030044856
    Abstract: A method of determining the presence of chronic volume dependent hypertension is provided wherein a determination is made as to whether there has been a substantial reduction in phosphorylation or concentration of a specific protein which may be a blood-derived protein or renal proximal brush border membrane protein and if such reduction exists concluding that chronic volume dependent hypertension exists in a patient. The method may advantageously be practiced by employing blood serum or blood plasma as the body specimen containing the protein in determining whether a patient has chronic volume dependent hypertension, a cellular component of the blood, such as a blood-derived protein coming from the plasma membrane of lymphocytes. The method may include subsequent therapeutic patient treatment. Related diagnostic apparatus is also provided. The blood-derived protein may be CLAMP.
    Type: Application
    Filed: November 21, 2001
    Publication date: March 6, 2003
    Inventor: Jules B. Puschett
  • Publication number: 20030044857
    Abstract: It is described a process for the preparation of ECP from nasal mucosa of a subject; the ECP protein so obtainable; and a method for monitoring the phlogosis from allergic reaction of a subject.
    Type: Application
    Filed: August 16, 2002
    Publication date: March 6, 2003
    Inventors: Francesco Marcucci, Franco Frati
  • Publication number: 20030044858
    Abstract: The present invention describes IgE antagonists (including variant anti-IgE antibodies) and their use in diagnosis, therapy or prophylaxis of allergic and other IgE-mediated disorders, including asthma, food allergies, hypersensitivity and anaphylactic reactions.
    Type: Application
    Filed: August 8, 2001
    Publication date: March 6, 2003
    Applicant: Genentech, Inc.
    Inventors: Paula M. Jardieu, Leonard G. Presta
  • Publication number: 20030044859
    Abstract: This invention relates to the use of two or more breast specific markers for the detection of breast cancer in a patient.
    Type: Application
    Filed: October 11, 2001
    Publication date: March 6, 2003
    Inventors: Jerry G. Henslee, Paula N. Friedman
  • Publication number: 20030044860
    Abstract: Methods of predicting a propensity to developing prostate cancer are presented. The method consists of measuring the IGF status of individual. Individuals with high IGF status, as compared with normal reference range values, are at increased risk for developing prostate cancer. More particularly, the IGF status may be determined by measuring IGF-I levels and/or IGFBP-3 levels. High IGF and low IGFBP levels are indicative of a high IGF status. A method of determining the prognosis of existing prostate cancers or of monitoring disease progression involves determining the IGF/PSA status of an individual. Individuals with a high IGF/PSA status (both high IGF status and high PSA levels) tend to develop severe prostate cancer and have a poorer overall prognosis.
    Type: Application
    Filed: December 18, 2001
    Publication date: March 6, 2003
    Applicant: Lady Davis Institute
    Inventors: Michael N. Pollak, Meir J. Stampfer, Edward Giovannucci
  • Publication number: 20030044861
    Abstract: This invention provides a method for diagnosing a patient with neoplasia.
    Type: Application
    Filed: April 22, 2002
    Publication date: March 6, 2003
    Inventors: Li Liu, Bing Zhu, Han Li, W. Joseph Thompson, Gary A. Piazza, Rifat Pamukcu
  • Publication number: 20030044862
    Abstract: Osteopontin (OPN) levels measured in bodily fluids of cancer patients are used as a noninvasive marker for tumor hypoxia as described herein. Methods of using OPN levels for the diagnosis, prognosis and treatment of cancers characterized by the presence of hypoxic cells, and a kit for detecting the presence of a hypoxic tumor are provided.
    Type: Application
    Filed: June 18, 2002
    Publication date: March 6, 2003
    Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JR. UNIVERSITY
    Inventors: Amato J. Giaccia, Quynh Thu Le, Nicholas C. Denko
  • Publication number: 20030044863
    Abstract: Therapeutics have been identified and developed with are targeted to inhibiting metastasis. These are based on the discovery of an invasion complex which confers the ability of cells, for example tumor cells, to translocate across extracellular matrix barriers, as well as the identification of novel peptides that interact with the invasion complex and regulate it's activity. Whole or partial complexes, or individual molecules of the invasion complex are used to screen for proteins or compounds that interact with the invasion complex. Methods of screening for interacting proteins such as osteopontin, sophin B (SEQ ID NO: 1), or compounds are well known in the art, some of which are described below. Once interacting proteins have been identified, they are screened for inhibition, enhancement, or reduction of complex activity.
    Type: Application
    Filed: July 18, 2002
    Publication date: March 6, 2003
    Applicant: Children's Medical Center Corporation
    Inventor: Samy Ashkar