Abstract: A method of selecting nucleic acid samples including particular desired alleles from a plurality of nucleic acid samples including the steps of performing a first reaction in a plurality of pools containing the samples to produce reaction products including a source tag identifying said each pool; pooling the pools to provide pooled pools; for each of the desired alleles to be identified, performing a second reaction using said reaction products to produce allele-specific second reaction products comprising a marker tag and a derived source tag; identifying said allele-specific second reaction products, and further identifying nucleic acid samples with the desired alleles. A source tag sharing number “d” may be determined for each of the alleles. Alleles may also be binned together.

Abstract: The invention provides methods and devices for detecting, enumerating or identifying target nucleic acid molecules using immobilized capture probes and single molecule sequencing techniques.

Abstract: A method and system for characterization of antibody-bound targets by sequencing of synthetic oligonucleotides bound to the antibodies, the method including: receiving a sample having a set of antibodies; conjugating each of the set of antibodies with an oligonucleotide, thereby generating a set of oligonucleotide-conjugated antibodies; binding a first subset of the set of oligonucleotide-conjugated antibodies to a set of targets at a capture substrate; determining a sequence for at least one of: 1) each oligonucleotide of the first subset of oligonucleotide-conjugated antibodies that bind to the set of targets and 2) each oligonucleotide of a second subset of the set of oligonucleotide-conjugated antibodies that fail to bind to the set of targets; and generating an analysis of the sample from the sequences determined from at least one of the first and the second subsets of oligonucleotide-conjugated antibodies.

Abstract: This disclosure provides systems and methods for sample processing and data analysis. Sample processing may include nucleic acid sample processing and subsequent sequencing. Some or all of a nucleic acid sample may be sequenced to provide sequence information, which may be stored or otherwise maintained in an electronic storage location. The sequence information may be analyzed with the aid of a computer processor, and the analyzed sequence information may be stored in an electronic storage location that may include a pool or collection of sequence information and analyzed sequence information generated from the nucleic acid sample. Methods and systems of the present disclosure can be used, for example, for the analysis of a nucleic acid sample, for producing one or more libraries, and for producing biomedical reports. Methods and systems of the disclosure can aid in the diagnosis, monitoring, treatment, and prevention of one or more diseases and conditions.

Abstract: The present invention relates to the use of Neutrophil Gelatinase-Associated Lipocalin (NGAL) and/or SERPINA3 as biomarkers of the Mineralocorticoid Receptor (MR) activation in a patient. More particularly, the present invention relates to a method for predicting the responsiveness of a patient to a treatment with a MR antagonist or an aldosterone synthase inhibitor, said method comprising determining in a biological sample obtained from said patient the expression level of the NGAL gene and/or of the SERPINA3 gene.

Abstract: The present invention relates to methods of detecting chromosomal abnormalities in a biological sample using flow cytometry and nucleic acid probes to assist with the same.

Abstract: Described herein are method, compositions and kits for prognosis of prostate cancer. The methods include determining the ratio of PCA3 and of a prostate-specific marker expression in a urine sample and correlating the value of the PCA3/prostate-specific marker ratio with the aggressiveness and mortality risk of prostate cancer in the subject. The method for prognosing prostate cancer in a sample of a patient includes assessing the amount of a prostate cancer specific PCA3 mRNA and the amount of prostate-specific marker in the sample; determining a ratio value of this amount of prostate cancer specific PCA3 mRNA over the amount of prostate-specific marker; comparing the ratio value to at least one predetermined cut-off value, wherein a ratio value above the predetermined cut-off value is indicative of a higher risk of mortality of prostate cancer as compared to a ratio value below the predetermined cut-off value.

Abstract: Methods and kits are provided for screening a patient for bladder cancer. Embodiments include those involving centromeric probes to chromosomes 3, 7, and 10 and a locus-specific probe to 5p15.

Abstract: In some embodiments, the disclosure relates generally to methods, as well as related systems, compositions, kits, apparatuses and computer-readable media, comprising a multiplex molecular tagging procedure that employs a plurality of tags that are appended to a plurality of polynucleotides. The tags have characteristics, including a sequence, length and/or detectable moiety, or any other characteristic, that uniquely identifies the polynucleotide molecule to which it is appended, and permits tracking individual tagged molecules in a mixture of tagged molecules. For example, the tag having a unique tag sequence, can uniquely identify an individual polynucleotide to which it is appended, and distinguish the individual polynucleotide from other tagged polynucleotides in a mixture. In some embodiments, the multiplex molecular tagging procedure can be used for generating error-corrected sequencing data and for detecting a target polynucleotide which is present at low abundance in a nucleic acid sample.

Abstract: Personalized medicine involves the use of a patient's molecular markers to guide treatment regimens for the patient. The scientific literature provides multiple examples of correlations between drug treatment efficacy and the presence or absence of molecular markers in a patient sample. Methods are provided herein that permit efficient dissemination of scientific findings regarding treatment efficacy and molecular markers found in patient tumors to health care providers.

Abstract: The present invention encompasses compositions and methods for detecting cancer metastasis.

Abstract: A method for analyzing short tandem repeats (STRs) is described herein. In some embodiments, the method comprises: separately digesting two portions of a genomic sample at sites that are upstream and downstream of an STR; fragmenting those products; ligating adaptors to the fragmentation products; selectively amplifying part of the top strand but not the bottom strand of the ligation products derived from the first portion, and part of the bottom strand but not the top strand of the ligation products derived from the second portion; sequencing at least some of the amplification products to produce a plurality of top strand reads and a plurality of bottom strand reads; and counting the number of STR repeats in a sequence read. A kit for performing the method is also provided.

Abstract: The invention discloses means and methods for genotyping an individual head of cattle. Individual's DNA is genotyped utilising the herein defined PCR SNaPshot protocol. The protocol comprises two PCR steps where the first step (PCR1) includes adding primers (SEQ ID No. 1-30) and/or primers extended at their 5? end with a common 10 base motif (ACGTTGGATG) to the PCR1 reaction. The second step (PCR2) includes adding extension primers (SEQ ID No. 31-45), and/or primers adjacent to corresponding specific SNPs. Further steps include producing amplicons from a PCR1 mixture comprising template DNA and the first primer set to yield PCR1 products, using PCR1 products as templates to a set of extension primers to yield PCR2 products. Size and colour separation is achieved by adding tails of different lengths to the PCR2 primers. PCR2 products are separated and the results compared with SNP profiles from the databank to obtain matching.

Abstract: This invention relates to novel nucleic acid probes and methods for detecting Plasmodium parasites as well as detecting different Plasmodium parasites selectively from one another.

Abstract: The present invention relates to methods and compositions for identifying, selecting and/or producing a soybean plant or germplasm having iron deficiency chlorosis tolerance. A soybean plant, part thereof and/or germplasm, including any progeny and/or seeds derived from a soybean plant or germplasm identified, selected and/or produced by any of the methods of the present invention is also provided.

Abstract: The present invention is directed towards a novel virus, called Cedar Virus, and its methods of use.

Abstract: The present invention provides processes for catalytically converting biomass to oxygenated compounds suitable for use in bioreforming processes.

Abstract: An apparatus includes rails for supporting sides of a circumferential peripheral edge of a carrier. A movable ram engages a rearward portion of the carrier. A movable restrainer restrains movement of the ram. A wheel engages a forward portion of the carrier. A controller is operable to control the movable restrainer to restrain movement of the ram with the carrier and at least one metal object disposed adjacent an induction heating device, the wheel to rotate the carrier and the metal object, the induction heating device to induction heat the rotating metal object, and the movable restrainer to disengage the ram so that the wheel pivots away to allow passage of the carrier and the heated metal object into a quench bath. A plurality of metal objects may be arranged in a circular array in the carrier about a diameter generally equal to the diameter of an induction coil.

Abstract: A quench system for applying cooling air to one or more hot metallic components that are supported on a component support having a substantially open construction. The quench system includes a housing having sidewalls that define a cooling chamber with peripheral portions proximate the sidewalls and a center portion spaced inwardly from the sidewalls. The quench system also includes a conveyance system that is configured to carry the component support into the center portion of the cooling chamber, as well as a forced air fan that generates a bulk flow of cooling air through the cooling chamber. The quench system further includes a plurality of nozzle baffles extending inwardly from the plurality of sidewalls to define a narrowing region within the housing between the forced air fan and the conveyance system, whereby, during operation of the fan, cooling air flowing through the peripheral portions of the cooling chamber is redirected into the center portion of the cooling chamber.

Abstract: A method for producing hot-rolled seamless pipes from transformable steel for pipelines in which the pipe ends are hot-upsetted in order to achieve a thickened wall portion after a final rolling process of the pipes to provide pipes with excellent fatigue, corrosion, and welding properties. A pre-selected ratio between a wall thickness of the pipe end and a wall thickness of a wall body adjoining the pipe end is set by the hot-upsetting process such that a pipe is achieved with a pipe end which has a lower strength than the pipe body after a uniform tempering process of the entire pipe following the hot-upsetting process by using a previously ascertained wall thickness-dependent cooling rate during the tempering process.

Abstract: A high-strength steel sheet has excellent workability, excellent phosphatability, and excellent corrosion resistance after electrodeposition coating has been performed, even when the contents of Si and Mn are high. Condition 1 through Condition 3 below are used when continuous annealing is performed on a steel sheet. Condition 1: In a heating process of continuous annealing, a steel sheet is heated at a heating rate of 7° C./s or more in a temperature range in an annealing furnace of 450° C. or higher and A° C. or lower (A: 500?A). Condition 2: The maximum end-point temperature of a steel sheet is 600° C. or higher and 750° C. or lower in continuous annealing. Condition 3: In a steel sheet temperature range of 600° C. or higher and 750° C. or lower in continuous annealing, the traveling time of the steel sheet through the temperature range is 30 seconds or more and 10 minutes or less, and the dew point of the atmosphere is ?10° C. or higher in the temperature range.

Abstract: A steel sheet for crown caps includes C: 0.0005 to 0.0050%, Si: 0.020% or less, Mn: 0.10 to 0.60%, P: 0.020% or less, S: 0.020% or less, Al: 0.01 to 0.10%, N: 0.0050% or less, Nb: 0.010 to 0.050%, and Fe and inevitable impurities. The steel sheet has an average r value of 1.30 or more and a YP of 450 MPa or more and 650 MPa or less. The steel sheet is obtained by hot rolling a steel slab at a slab reheating temperature of 1,150° C. or higher and a finishing temperature of 870° C. or higher; coiling the hot rolled steel sheet at a coiling temperature of 600° C. or higher, performing pickling; then-performing primary cold rolling; performing annealing at an annealing temperature of recrystallization temperature or more and 790° ° C. or less; and performing secondary cold rolling at a rolling reduction of 10% or more and 50% or less.

Abstract: The present invention provides a steel sheet for a rotor core for an IPM motor, wherein the steel sheet has a magnetic flux density B8000 of 1.65 T or more as measured when magnetic field strength is 8000 A/m, and a residual magnetic flux density Br of 0.5 T or more as measured at that time, and optionally, a coercivity Hc of 100 A/m or more as measured after magnetization reaches 8000 A/m. By using the steel sheet of the present invention for a rotor core of an IPM motor, it is possible to increase further an output torque in a high-speed rotational range and raise further the maximum rotational speed.

Abstract: Provided is an HPF member having excellent bending characteristics and a hot-dip aluminum plated layer formed on a surface of a base steel sheet. The base steel sheet includes, in terms of wt %, 0.18-0.25% of C, 0.1-0.5% of Si, 0.9-1.5% of Mn, 0.03% or less of P, 0.01% or less of S, 0.01-0.05% of Al, 0.05-0.5% of Cr, 0.01-0.05% of Ti, 0.001-0.005% of B, 0.009% or less of N, the balance Fe and other impurities; a ferrite phase having a thickness within 50 ?m is continuously or discontinuously formed inside a surface portion of the base steel sheet; the fraction of the surface portion occupied by the ferrite phase is 5% or less; and carbide materials having a size of 1 ?m or less are scattered and distributed in the surface portion of the base steel sheet so as to occupy 90% or more of the overall carbide distribution.

Abstract: Provided is a hot press-formed (HPF) member with excellent powdering resistance at the time of press forming. The HPF member includes a hot-dip coating layer containing Al on a surface of a base steel sheet. The base steel sheet includes, based on wt %, 0.18-0.25% of C, 0.1-1.0% of Si, 0.9-1.5% of Mn, 0.03% or less of P, 0.01% or less of S, 0.01-0.05% of Al, 0.05-0.5% of Cr, 0.01-0.05% of Ti, 0.001-0.005% of B, 0.009% or less of N, the balance Fe, and the other impurities. The hot-dip coating layer comprises a soft diffusion layer and a hard alloy layer. The alloy layer has a Tau phase in the range of 10-30%, in terms of area percent.

Abstract: A furnace for thermal treatment with gaseous atmosphere quenching having a bell inside which there are provided a rotor regulating the gas atmosphere flow, a heat exchanger regulating the temperature of the gas atmosphere and a thermal chamber configured for thermal treatment and the following gaseous atmosphere quenching. The thermal chamber has at least a surface positioned on a side adjacent to the heat exchanger and at least a surface positioned on an opposed side to the adjacent one to the heat exchanger and wherein at least a surface has a plurality of screens which connect the inside of the thermal chamber with the inside of the bell to allow the passage of the gas atmosphere from the thermal chamber to the bell, wherein the screens are adjustable to modify the passage section and so the flow of the gas atmosphere in function of the temperature required by the thermal treatment.

Abstract: A method of treating rare earth metal-bearing permanent magnet scrap, waste or other material in a manner to recover the heavy rare earth metal content separately from the light rare earth metal content. The heavy rare earth metal content can be recovered either as a heavy rare earth metal-enriched iron based alloy or as a heavy rare earth metal based alloy.

Abstract: The invention relates to a lead-free bismuth-free silicon-free brass alloy, comprising: by the total weight of the brass alloy, 60-65 wt % copper, 0.01-0.15 wt % antimony, 0.1-0.5 wt % magnesium, one or more element selected from the group consisting of 0.1-0.7 wt % aluminum, 0.05-0.5 wt % tin, 0.05-0.3 wt % phosphorus, 0.05-0.5 wt % manganese and 0.001-0.01 wt % boron, and a balance of zinc. The brass alloy of the invention does not adopt lead, thus avoiding lead pollution. Besides, neither bismuth nor silicon is adopted, thus enabling the brass alloy to have an improved cutting performance.

Abstract: A wear-resistant alloy having a complex microstructure, which may include a range of about 8 to about 17 wt % of zinc (Zn), a range of about 5 to 8 wt % of tin (Sn), a range of about 9.4 to about 12.6 wt % of silicon (Si) and a balance of aluminum (Al), is provided.

Abstract: A particulate feedstock for an additive manufacturing process includes particles formed from an aluminum base alloy. The alloy includes both aluminum and copper, and the amount of aluminum in the alloy is greater than the amount of copper in the alloy as a percentage of total weight. The alloy also includes at least one other material including at least one of magnesium manganese, titanium, nickel, and boron. The amount of copper in the alloy is greater than the amounts of each of the magnesium, manganese, titanium, nickel, and/or boron included in the alloy to render the particulate amenable to high energy density joining techniques.

Abstract: The present invention provides a steel strip for cutlery, which has a composition containing, in mass %, 0.45 to 0.55% of C, 0.2 to 1.0% of Si, 0.2 to 1.0% of Mn, and 12 to 14% of Cr, and further contains Mo, with the balance made up of Fe and unavoidable impurities, in which Mo is contained in an amount of 2.1 to 2.8%, and the amount of formed M3C deposited by tempering is decreased to improve bending workability.

Abstract: An aluminum alloy extruded material exhibits excellent hardenability that ensures that high strength can be obtained by air-cooling immediately after extrusion and artificial aging, and exhibits excellent formability (e.g., press formability). An aluminum alloy includes 0.30 to 1.00 mass % of Mg, 0.6 to 1.40 mass % of Si, 0.10 to 0.40 mass % of Fe, 0.10 to 0.40 mass % of Cu, 0.005 to 0.1 mass % of Ti, and 0.3 mass % or less of Mn, with the balance being aluminum and unavoidable impurities, the aluminum alloy having a stoichiometric Mg2Si content of 0.60 to 1.30 mass % and an excess Si content of 0.30 to 1.00 mass %.

Abstract: The present invention provides a superelastic alloy formed by addition of Fe or Co to an Au—Cu—Al alloy, including: Cu of 12.5% by mass or more and 16.5% by mass or less; Al of 3.0% by mass or more and 5.5% by mass or less; Fe or Co of 0.01% by mass or more and 2.0% by mass or less; and a balance Au, and a difference between Al content and Cu content (Cu—Al) is 12% by mass or less. The superelastic alloy according to the present invention has superelastic property while being Ni-free, excellent X-ray imaging property, processability, and strength property, and is suitable for a medical field.

Abstract: A method for reducing surface roughness of an article includes contacting a surface of an article with a molten metal agent, the surface having an initial roughness; altering at least a portion of the surface in the molten metal agent; and removing the surface from contact with the agent; wherein, after the removing step, the surface has a processed roughness that is less than the initial roughness.

Abstract: A coated substrate comprises: a metallic substrate; a bondcoat atop the substrate; and a ceramic barrier coat atop the bondcoat. The bondcoat has a combined content of one or more of molybdenum, chromium, and vanadium of at least 50 percent by weight.

Abstract: A process for coating a component including applying a thermal barrier coating material to a bond coat and selectively infiltrating a blocking material into the thermal barrier coating material.

Abstract: A sprayed coating forming method of forming a sprayed coating includes pre-heating a cylinder block defining a plurality of cylinders, supplying air into an enclosure that houses the cylinder block, inserting a spray gun sequentially into cylinder bores formed in the cylinder block while discharging the air at least from one side of the cylinder block, and spraying droplets of a molten metal onto an inner surface of each of the cylinder bores. The spraying includes spraying one of the cylinder bores first, then next spraying another of the cylinder bores that has at least one cylinder disposed between the another of the cylinder bores and the one of the cylinder bores sprayed first. The air flows around the cylinder block and also flows inside the cylinder bores.

Abstract: Described are articles having, at least, an outer surface comprising unalloyed oxidized Zirconium with Niobium having a thickness and configurations suitable for sports equipment, golf clubs, aircraft, boat hulls, motor vehicle, propellers, helicopter rotors, and various other non-medical applications.

Abstract: A vacuum evaporation apparatus comprises a housing, in which a main vacuum cavity, a side vacuum cavity and a switch assembly are disposed. The main vacuum cavity is configured for vacuum evaporation. A tomography detection module for scanning a crucible to be measured and a manipulator for accessing a crucible in main vacuum cavity are disposed in the side vacuum cavity. A switch assembly is disposed between the main vacuum cavity and the side vacuum cavity. Where the switch assembly is opened, the main vacuum cavity and the side vacuum cavity are communicated with each other to enable accessing the crucible. Where the switch assembly is closed, the main vacuum cavity and the side vacuum cavity are separated from each other.

Abstract: The present invention relates to a technical field of display, and discloses a substrate carrying apparatus. The apparatus comprises: a vertical bearing plate and an upper holding base; one side surface of the vertical bearing plate is a bearing surface, the upper holding base is provided at the upside of the bearing surface; the upper holding base is provided with a plurality of holder for holding the substrate; the left and right sides of the bearing surface are provided with a plurality of positioning clips for positioning the substrate from the left and right ends of the substrate. The substrate carrying apparatus of the present invention provides a holder on the upper holding base, and during loading of the substrate, after the substrate is transported in place by a manipulator, the substrate is held and hanged by the holder, and the substrate would not undergo a falling process, reducing the risk of substrate damage.

Abstract: A cover for a configurable measuring system of a configurable sputtering system which is adapted for sputtering multilayer coatings with varying compositions and comprising a plurality of sputtering zones and having a plurality of apertures on which the cover is detachably attachable, and wherein the cover comprises a sensor system for in situ detection of a property of the multilayer coating on a substrate, wherein said at least one sensor system is attached to the cover.

Abstract: A method of modifying substrate surface includes: performing an O2 plasma treatment on a substrate including polydimethylsiloxane (PDMS); coating hydrophilic UV curing coating uniformly on the substrate; disposing the substrate into an oxygen-free environment; and exposing to an UV light to cure the hydrophilic UV curing coating. The method of modifying substrate surface may greatly enhance the hydrophilicity and the stability of the PDMS substrate.

Abstract: A gas dispenser utilized in a deposition apparatus is provided. The gas dispenser includes a showerhead comprising a plurality of holes, and a mask layer formed on a surface of the showerhead, wherein the holes penetrate through the mask layer. A deposition apparatus using the gas dispenser is also disclosed.

Abstract: A reactor system and related methods are provided which may include a heating element in a wafer tray. The heating element may be used to heat the wafer tray and a substrate or wafer seated on the wafer tray within a reaction chamber assembly, and may be used to cause sublimation of a native oxide of the wafer.

Abstract: A method of manufacturing a semiconductor device includes (a) under a first process condition, supplying a purge gas containing hydrogen and oxygen to a process chamber from which an oxide film adhering to an internal member is removed by a cleaning gas containing a halogen element and exhausting the purge gas from the process chamber; and (b) under a second process condition differing from the first process condition, supplying the purge gas to the process chamber and exhausting the purge gas from the process chamber after performing (a), wherein the first process condition is a process condition under which reactivity of the halogen element, which remains within the process chamber when the cleaning gas is supplied to the process chamber, with the purge gas is higher than reactivity of the halogen element with the purge gas under the second process condition.

Abstract: An apparatus for manufacturing semiconductor devices having a gas mixer includes a gas supply and a reaction chamber, and the gas supply includes an upper gas mixer, an intermediate gas mixer disposed under the upper gas mixer, a lower gas mixer disposed under the intermediate gas mixer, a first gas supply pipe which is disposed on an upper portion of the upper gas mixer and supplies a first gas to the upper gas mixer, a second gas supply pipe which is disposed on an upper end portion of a side surface of the upper gas mixer and supplies a second gas to the upper gas mixer, and a third gas supply pipe which is disposed on a side surface of the intermediate gas mixer and supplies a third gas to the intermediate gas mixer.

Abstract: Disclosed are a precursor composition for forming a zirconium-containing film and a method for forming a zirconium-containing film by using the same, wherein the composition is characterized in that about 1 to 3 moles of a cycloaliphatic unsaturated compound represented by a particular chemical formula or an aromatic compound represented by a particular chemical formula: and about 1 to 3 moles of a cyclopentadienyl zirconium (IV)-based compound represented by a particular chemical formula are mixed. In the composition, the two constituent compounds are stable with each other and homogenously mixed with each other in a liquid state, without reacting with each other, and thus the composition behaves just like a single compound, and exhibits a high vapor pressure. The use of the composition of the present invention can obtain a zirconium-containing film, like high-quality zirconia, conveniently and economically.

Abstract: Systems for a managing a chemical process and photoionization detectors for analyzing a process gas are presented. In one aspect, the system includes a process gas source in fluid communication with the process chamber and a photoionization detector. The photoionization detector is configured to analyze the process gas. The photoionization detector includes a heat resistant coupling for connection to the system, a gas sample chamber with the radiation window soldered or brazed to a wall of the gas sample chamber, and a radiation source configured to emit radiation through the radiation window and into the gas sample chamber to analyze the process gas. In another aspect, the photoionization detector includes a removable coupling, a gas sample chamber, and a radiation source. The removable coupling is for connection to the process gas handling system and includes a metal gasket and metal flanges.

Abstract: The present disclosure relates to methods and apparatus for an atomic layer deposition (ALD) processing chamber for device fabrication and methods for replacing a gas distribution plate and mask of the same. The ALD processing chamber has a slit valve configured to allow removal and replacement of a gas distribution plate and mask. The ALD processing chamber may also have actuators operable to move the gas distribution plate to and from a process position and a substrate support assembly operable to move the mask to and from a process position.

Abstract: Disclosed is a substrate processing method including: placing a plurality of substrates on a rotary table in a processing container; and performing a processing on the substrates while rotating the rotary table. A dummy workpiece is disposed in a gap between the substrates placed on the rotary table.