Patents Issued in January 3, 2017
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Patent number: 9534209Abstract: The present invention concerns 3D-crystals of complexes of ribosomal S6 kinase (RSK) and mitogen- and stress-activated protein kinase (MSK) proteins and their ligands, as well as methods for crystallization, three-dimensional structure determination and structure assisted methods for identifying ligands of said proteins.Type: GrantFiled: September 30, 2013Date of Patent: January 3, 2017Assignees: Aarhus Universitet, Region MidtjyllandInventors: Jacob Lauwring Andersen, Borbala Gesser, Poul Nissen, Lars Iversen
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Patent number: 9534210Abstract: The present invention relates to a reverse transcriptase having improved thermostability, more precisely a mutant reverse transcriptase with improved thermostability by substitution of one or more amino acids selected from the group consisting of the 63rd glutamine (Q63), the 264th lysine (K264), the 295th lysine (K295), the 306th threonine (T306), the 346th glutamic acid (E346), the 408th proline (P408), the 438th histidine (H438), and the 454th asparagin (N454) of the amino acid sequence of M-MLV originated reverse transcriptase represented by SEQ. ID. NO: 1 with other amino acids. The mutant reverse transcriptase of the present invention demonstrates excellent thermostability, compared with the wild type reverse transcriptase. Therefore, it is advantageous to obtain the target cDNA with stable reverse transcription activity even in the presence of RNA that can form the stable secondary structure at a high temperature.Type: GrantFiled: February 7, 2012Date of Patent: January 3, 2017Assignee: BIONEER CORPORATIONInventors: Han Oh Park, Sung Jun Yang, Sung Mo Joo, Byoung Oh Hwang
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Patent number: 9534211Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: November 5, 2010Date of Patent: January 3, 2017Assignees: NOVOZYMES A/S, NOVOZYMES, INC.Inventors: Elena Vlasenko, Brett McBrayer, Dominique Skovlund, Sara Landvik
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Patent number: 9534212Abstract: The present disclosure is directed to compositions comprising heat-inactivated complement factor B and methods of using the same to treat thrombotic or complement-mediated inflammatory disorders.Type: GrantFiled: February 25, 2015Date of Patent: January 3, 2017Assignee: WILLIAM MARSH RICE UNIVERSITYInventor: Nancy A. Turner
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Patent number: 9534213Abstract: Supraparticle nanoassemblies are provided that comprise nanoparticle species and protein species, as well as methods for making such assemblies. A supraparticle may comprise a nanoparticle species with a first charge and an average particle size diameter of ?about 1 nm to ?about 100 nm. The supraparticle also comprises a protein species. The nanoparticle species and the protein species have the same charge and are assembled together without any intramolecular chemical bonding to form the supraparticle. The supraparticle may be a substantially round particle. In certain other aspects, a photoreactive supraparticle is provided, where the nanoparticle is reactive to energy or electromagnetic radiation, which in the presence of such energy or radiation enhances reactivity of the protein species in the supraparticle.Type: GrantFiled: March 2, 2015Date of Patent: January 3, 2017Assignee: The Regents Of The University Of MichiganInventor: Nicholas A. Kotov
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Patent number: 9534214Abstract: A solid substrate for biological sample storage under dry-state and elution of biomolecules is provided. The dry, solid substrate is coated with saccharides, such as monosaccharides, oligosaccharides, polysaccharides or combinations thereof, and the substrate is comprised of one or more protein denaturing agents impregnated therein under a substantially dry state. A method for elution of biomolecules from biological samples is also provided. The compositions disclosed herein provide for enhanced elution and recovery of biomolecules, such as nucleic acids, from the sample. The sample is disposed on a substrate, dried to a substantially dry state; eluted from the biological sample dried on the substrate by rehydrating the substrate in an elution buffer.Type: GrantFiled: October 31, 2013Date of Patent: January 3, 2017Assignee: General Electric CompanyInventors: Bing Li, Gregory Andrew Grossmann, Erik Leeming Kvam, Brian Christopher Bales, Jason Louis Davis
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Patent number: 9534215Abstract: A method of separating bead substrates includes applying an emulsion to an emulsion-breaking solution. A dispersed phase of the emulsion includes an unbound polynucleotide, a first set of bead substrates and a second set of bead substrates. The unbound polynucleotide includes a segment complementary to a coupling oligonucleotide. The first set of bead substrates includes the coupling oligonucleotide extended to include a segment complementary to a portion of the unbound polynucleotide. The second set of bead substrates includes the coupling oligonucleotide. The emulsion-breaking solution includes an interference probe having a sequence similar to the coupling oligonucleotide or complementary to the coupling oligonucleotide. The method further includes binding beads of the first set of bead substrates to separation substrates and separating unbound beads of the second set of bead substrates from the beads of the first set of bead substrates bound to the separation substrates.Type: GrantFiled: June 11, 2015Date of Patent: January 3, 2017Assignee: Life Technologies CorporationInventor: Brian Reed
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Patent number: 9534216Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.Type: GrantFiled: April 9, 2014Date of Patent: January 3, 2017Assignee: Raindance Technologies, Inc.Inventors: Darren R. Link, Laurent Boitard, Jeffrey Branciforte, Yves Charles, Gilbert Feke, John Q. Lu, David Marran, Ahmadali Tabatabai, Michael Weiner, Wolfgang Hinz, Jonathan M. Rothberg
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Patent number: 9534217Abstract: The present invention relates to a method of creating DNA libraries that include an artificial promoter library and/or a modified ribosome binding site library and transforming bacterial host cells with the library to obtain a population of bacterial clones having a range of expression levels for a chromosomal gene of interest.Type: GrantFiled: June 7, 2011Date of Patent: January 3, 2017Assignee: DANISCO US IN.Inventors: Philippe Soucaille, Marguerite A. Cervin, Fernando Valle
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Patent number: 9534218Abstract: Compositions and methods for improving expression of a recombinant protein or polypeptide of interest in a host cell are provided. Compositions comprising a polynucleotide coding sequence for a BTBooster are provided using a plant optimized system. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a recombinant protein or polypeptide of interest in a host cell. Methods comprising the codon optimization of a polynucleotide coding sequence for increased expression in a bacterial host are provided. The codon optimization method can be used in designing a polynucleotide coding sequence which expresses robust levels of protein in a bacterial host cell.Type: GrantFiled: June 13, 2013Date of Patent: January 3, 2017Assignee: Dow AgroSciences LLCInventors: Matthew Kelker, Aaron T. Woosley
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Patent number: 9534219Abstract: Provided are methods of treating or delaying the onset of a vascular inflammatory disease (e.g., acute lung injury) in a subject including administering to the subject a therapeutically effective amount of a nucleic acid containing all or a part of the sequence of mature miR-181b (SEQ ID NO: 1). Also provided are methods of decreasing nuclear factor-?? (NF-??) signaling in an endothelial cell including administering to the subject a nucleic acid containing all or a part of the sequence of mature miR-181b (SEQ ID NO: 1).Type: GrantFiled: December 5, 2014Date of Patent: January 3, 2017Assignee: The Brigham and Women's Hospital, Inc.Inventors: Mark W. Feinberg, Xinghui Sun
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Patent number: 9534220Abstract: A method and antisense compound for inhibiting the growth of pathogenic bacterial cells are disclosed. The compound contains no more than 12 nucleotide bases and has a targeting nucleic acid sequence of no fewer than 10 bases in length that is complementary to a target sequence containing or within 10 bases, in a downstream direction, of the translational start codon of a bacterial mRNA that encodes a bacterial protein essential for bacterial replication. The compound binds to a target mRNA with a Tm of between 50° to 60° C. The relatively short antisense compounds are substantially more active than conventional antisense compounds having a targeting base sequence of 15 or more bases.Type: GrantFiled: November 19, 2012Date of Patent: January 3, 2017Assignee: Sarepta Therapeutics, Inc.Inventors: Bruce L. Geller, Jesse D. Deere, Patrick L. Iversen
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Patent number: 9534221Abstract: The present invention relates to methods and compositions for treating and/or preventing allergic diseases or conditions by inhibiting one or more components of the steroidogenic pathway.Type: GrantFiled: January 22, 2014Date of Patent: January 3, 2017Assignee: National Jewish HealthInventors: Erwin W. Gelfand, Meiqin Wang, Yi Jia
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Patent number: 9534222Abstract: Various methods and compositions relating to vascular endothelial growth factor receptor 2 (VEGFR2) are provided. In one aspect, a method of increasing expression of the soluble form of VEGFR2 (sVEGFR2) in a subject can include binding an antisense morphoiino to an exon13-intron13 splicing site of VEGFR2 mRNA such that the VEGFR2 niRNA is spliced into a sVEGFR2 isoform.Type: GrantFiled: November 15, 2012Date of Patent: January 3, 2017Assignee: University of Utah Research FoundationInventors: Balamurali K. Ambati, Hironori Uehara
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Patent number: 9534223Abstract: The present invention relates to methods of eliminating, reducing or preventing bacterial biofilms by means of a fusion protein comprising an endolysin, an autolysin or a bacteriocin to which a peptide with membrane or LPS disrupting activity is fused. Further, the present invention relates to fusion proteins for use as a medicament, in particular for the treatment or prevention of Gram-negative and/or Gram-positive bacterial infections associated with bacterial biofilm, as diagnostic means, disinfectant or as cosmetic substance. The present invention also relates to the removal or reduction or prevention of Gram-negative and/or Gram-positive bacterial contamination associated with bacterial biofilm of foodstuff, of food processing equipment, of food processing plants, of surfaces coming into contact with foodstuff, of medical devices, of surfaces in hospitals and surgeries.Type: GrantFiled: April 27, 2011Date of Patent: January 3, 2017Assignee: LYSANDO AGInventor: Stefan Miller
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Patent number: 9534224Abstract: The present invention provides nucleic acid molecules, DNA constructs, plasmids, and methods for post-transcriptional regulation of gene expression using RNA molecules to both repress and activate translation of an open reading frame. Repression of gene expression is achieved through the presence of a regulatory nucleic acid element (the cis-repressive RNA or crRNA) within the 5? untranslated region (5? UTR) of an mRNA molecule. The nucleic acid element forms a hairpin (stem/loop) structure through complementary base pairing. The hairpin blocks access to the mRNA transcript by the ribosome, thereby preventing translation. In particular, in embodiments of the invention designed to operate in prokaryotic cells, the stem of the hairpin secondary structure sequesters the ribosome binding site (RBS). In embodiments of the invention designed to operate in eukaryotic cells, the stem of the hairpin is positioned upstream of the start codon, anywhere within the 5? UTR of an mRNA.Type: GrantFiled: November 14, 2003Date of Patent: January 3, 2017Assignee: Trustees of Boston UniversityInventors: James J. Collins, Farren J. Isaacs, Charles R. Cantor, Daniel J. Dwyer
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Patent number: 9534225Abstract: This invention relates generally to a codon optimized nucleic acid encoding a retinitis pigmentosa GTPase regulator (RPGR) protein. The nucleic acid has enhanced stability during plasmid production relative to a wildtype cDNA encoding the RPGR protein. The invention also relates to expression cassettes, vectors, and host cells comprising the codon optimized nucleic acid. Methods for preparing a recombinant adeno-associated (rAAV) expression vector comprising the codon optimized nucleic acid sequence are also provided. The nucleic acids, expression cassettes, vectors, and host cells provided may be useful in the large scale production of rAAV expression vectors for gene therapy applications.Type: GrantFiled: April 15, 2015Date of Patent: January 3, 2017Assignee: Applied Genetic Technologies CorporationInventors: Guo-Jie Ye, Jilin Liu
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Patent number: 9534226Abstract: The invention pertains to an expression vector or a combination of at least two expression vectors comprising at least (a) a polynucleotide encoding a product of interest or an insertion site for incorporating a polynucleotide encoding a product of interest; (b) a polynucleotide encoding a first selectable marker (sm I); (c) a polynucleotide encoding a second selectable marker (sm II), which is different from the first selectable marker (sm I), wherein the activity of the selectable marker (sm I) or (sm II) is at least partially influenced by the activity of the other selectable marker and wherein the selectable markers (sm I) and (sm II) are involved in the folate metabolism. Also provided are suitable host cells, selection methods and methods for producing polypeptides with high yield.Type: GrantFiled: February 26, 2010Date of Patent: January 3, 2017Assignee: Novartis AGInventors: Thomas Jostock, Hans-Peter Knopf
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Patent number: 9534227Abstract: Provided are enhanced high yield production systems for producing terpenes in plants via the expression of fusion proteins comprising various combinations of geranyl diphosphate synthase large and small subunits and limonene synthases. Also provided are engineered oilseed plants that accumulate monoterpene and sesquiterpene hydrocarbons in their seeds, as well as methods for producing such plants, providing a system for rapidly engineering oilseed crop production platforms for terpene-based biofuels.Type: GrantFiled: May 13, 2013Date of Patent: January 3, 2017Assignee: Donald Danforth Plant Science CenterInventors: Toni Kutchan, Yasuhiro Higashi, Xiaohong Feng
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Patent number: 9534228Abstract: This invention relates to methods for altering cell proliferation in a plant by modulating the expression of an endogenous plant gene whose expression product affects cell proliferation. These methods can be used to modify plant characteristics, such as seed size. In particular, the invention provides methods for modifying cell proliferation in the integuments and/or seed coats of a plant by inhibiting expression of endogenous gene encoding Auxin response factor 2 (ARF2/MNT) or an orthologue thereof, such that the number of cells in the integuments and/or seed coat is increased, resulting in increased seed size. The plant or plant propagating material can be transformed, e.g., with a nucleic acid construct, to increase or decrease the expression of endogenous genes or the orthologues thereof that affect cell proliferation. The invention also relates to modified plants and reproducible plant material produced by the methods, and nucleic acid constructs for use in such methods.Type: GrantFiled: May 24, 2011Date of Patent: January 3, 2017Assignee: The University of BathInventor: Roderick Scott
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Patent number: 9534229Abstract: The present invention comprises recombinant DNA molecule, expression cassette, DNA vector, binary plasmid, plant cell and a method of polypeptide production in eukaryotic organism and use thereof. In more details, it provides the means, through using methods of genetic engineering, of obtaining plants with advantageous breeding features, particularly with increased tolerance to abiotic stresses including mineral deficiency or plants useful for monitoring the process of autophagy.Type: GrantFiled: October 27, 2011Date of Patent: January 3, 2017Assignee: INSTYTUT BIOCHEMII I BIOFIZYKI PANInventors: Katarzyna Zientara-Rytter, Grzegorz Moniuszko, Anna Wawrzyńska, Jolanta Łukomska, Agieszka Sirko
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Patent number: 9534230Abstract: Stress tolerance in plants and plant cells is achieved by using nucleotide sequences encoding enzymes involved in the NAD salvage synthesis pathway and/or the NAD de novo synthesis pathway e.g. for overexpression in plants.Type: GrantFiled: August 11, 2014Date of Patent: January 3, 2017Assignee: Bayer CropScience N.V.Inventors: Marc De Block, Michael Metzlaff, Véronique Gossele
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Patent number: 9534231Abstract: The present invention provides a method for improving the tolerance of a plant to an abiotic stress and a method for promoting growing of a plant, comprising a nucleotide sequence encoding the AtSRP (Arabidopsis thaliana stress related protein) of a plant. The present nucleotide sequence is involved in abiotic stress tolerance such as drought, low-temperature and salt stresses of plants. Therefore, the overexpressing transgenic plants have excellent tolerances to these abiotic stresses, whereby they may be useful as novel functional crops which are affected by climates and environments of the cultivated areas. In addition, where the plants are transformed with the present nucleotide sequence, the growth abilities of the transgenic plants are remarkably enhanced, whereby they may effectively used for cultivating the plants with novel function of rapid growing, and biomass.Type: GrantFiled: August 10, 2013Date of Patent: January 3, 2017Assignee: INDUSTRY-ACADEMIC COOPERATION FOUNDATION, YONSEI UNIVERSITYInventors: Woo Taek Kim, Eun Yu Kim, Ji Ho Seo, Ki Youl Park
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Patent number: 9534232Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran pests, and the plant cells and plants obtained thereby.Type: GrantFiled: October 5, 2012Date of Patent: January 3, 2017Assignee: Dow AgroSciences LLCInventors: Kenneth E. Narva, Huarong Li, Chaoxian Geng, Ignacio Larrinua, Monica Britt Olson, Navin Elango
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Patent number: 9534233Abstract: The present invention is related to a nucleic acid construct comprising an expression unit for the expression of E1B, wherein the expression unit comprises a promoter, a nucleotide sequence coding for E1B, and a 3?UTR, wherein the promoter is operatively linked to the nucleotide sequence coding for E1B, wherein the 3?UTR comprises 30 or less than 30 Exonic Enhancer Elements (ESEs), preferably 20 or less than 20 Exonic Enhancer Elements (ESEs), and wherein the 3? UTR is a non-viral 3? UTR.Type: GrantFiled: May 7, 2013Date of Patent: January 3, 2017Inventor: Stefan Kochanek
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Patent number: 9534234Abstract: The present invention provides polynucleotide vectors for high expression of heterologous genes, and methods for constructing such vectors. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, but not limited to, gene expression, gene therapy, insertional mutagenesis, or gene discovery.Type: GrantFiled: April 9, 2015Date of Patent: January 3, 2017Assignee: DNA2.0, INC.Inventors: Jeremy Minshull, Mark Welch, Sridhar Govindrajan, Kate Caves
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Patent number: 9534235Abstract: Methods of disrupting germ cell migration and development in a fish embryo by inducing targeted expression of Sdf-1a or Lif and disruption of the Sdf-1a gradient or a Lif signaling pathway in the fish embryo have been developed. Plasmid constructs containing a gene encoding Sdf-1a or Lif and a gene encoding a signaling sequence for targeted expression of Sdf-1a or Lif have been generated. The plasmids will be administered to a fish or a population of fish to reproductively sterilize the population with efficacy of up to 100%. Transgenic fish of this invention are reproductively incompetent of genetically contaminating a wild fish population.Type: GrantFiled: December 2, 2011Date of Patent: January 3, 2017Assignee: PURDUE RESEARCH FOUNDATIONInventors: Ten-Tsao Wong, Paul Collodi
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Patent number: 9534236Abstract: An example includes an apparatus, method, and system for producing and extracting a gas from a wastewater fluid. The apparatus can include a membrane including at least one hollow fiber, the at least one hollow fiber configured to convey a gas extracted from a wastewater fluid. Bacteria can be immobilized within the membrane, and the bacteria configured to produce the gas during treatment of the wastewater fluid.Type: GrantFiled: March 5, 2014Date of Patent: January 3, 2017Assignee: Regents of the University of MinnesotaInventors: Paige J. Novak, William A. Arnold, Erin M. Surdo
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Patent number: 9534237Abstract: The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.Type: GrantFiled: August 13, 2014Date of Patent: January 3, 2017Assignee: University of Kentucky Research FoundationInventors: Joseph Chappell, Bryan Greenhagen
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Patent number: 9534238Abstract: The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons.Type: GrantFiled: September 2, 2015Date of Patent: January 3, 2017Assignee: NOVOZYMES A/SInventors: Martin Hofrichter, Katrin Scheibner, Rene Ullrich, Matthias Kinne, Sebastian Peter, Henrik Lund, Lisbeth Kalum
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Patent number: 9534239Abstract: The present invention relates to glucoamylase variants having reduced sensitivity to protease nicking. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: September 5, 2012Date of Patent: January 3, 2017Assignee: NOVOZYMES A/SInventors: Tomoko Matsui, Suzanne Clark
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Patent number: 9534240Abstract: The present invention relates to a quinolinic acid-producing recombinant microorganism expressing a fusion protein of L-aspartate oxidase and quinolinate synthase linked via a linker, and a method for producing quinolinic acid using the same.Type: GrantFiled: January 3, 2013Date of Patent: January 3, 2017Assignee: CJ CHEILJEDANG CORPORATIONInventors: Yong Uk Shin, So Young Kim, In Kyung Heo, Ju Eun Kim, Sung Kwang Son, Jae Hee Lee, Ji Hyun Lee, Chang Gyeom Kim
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Patent number: 9534241Abstract: Aspects of the invention include host cells that are engineered to produce benzylisoquinoline alkaloids (BIAs). The host cells include heterologous coding sequences for a variety of enzymes involved in synthetic pathways from starting compounds to BIAs of the host cell. Also provided are methods of producing the BIAs of interest by culturing the host cells under culture conditions that promote expression of enzymes encoded by the heterologous coding sequences of the host cells. Aspects of the invention further include compositions, e.g., host cells, starting compounds and kits, etc., that find use in methods of the invention.Type: GrantFiled: March 14, 2014Date of Patent: January 3, 2017Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Christina D. Smolke, Catherine Thodey, Isis Trenchard, Stephanie Galanie
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Patent number: 9534242Abstract: Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce ethanol. Biomass feedstock is saccharified in a vessel by operation of a jet mixer, the vessel also containing a fluid medium and a saccharifying agent.Type: GrantFiled: June 19, 2015Date of Patent: January 3, 2017Assignee: XYLECO, INC.Inventors: Marshall Medoff, Thomas Craig Masterman
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Patent number: 9534243Abstract: A process for making a nucleotide analog includes combining a first substrate that includes a linker and a base with a second substrate to form a substrate composition. An enzyme contacts the substrate composition and catalyzes formation of the nucleotide analog from the first substrate and the second substrate. Additionally, a composition includes the first substrate, second substrate, the enzyme, the nucleotide analog, and optional additives.Type: GrantFiled: February 7, 2014Date of Patent: January 3, 2017Assignees: THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF COMMERCE, MEMORIAL SLOAN KETTERING CANCER CENTER, UNIVERSITY OF MARYLANDInventors: John P. Marino, Zvi Kelman, Jerard Hurwitz, Gary G. Giulian
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Patent number: 9534244Abstract: An electrochemical test sensor is adapted to measure glucose and correct for the oxygen effect in a fluid sample. The test sensor comprises a base, first and second working electrodes, and a counter electrode. The first working electrode includes glucose oxidase, a mediator and peroxidase. The second working electrode includes glucose oxidase and the mediator. The first working electrode, the second working electrode and the counter electrode are located on the base. In other embodiments, an electrochemical test sensor is adapted to measure cholesterol, lactate, pyruvate or xanthine and correct for the oxygen effect in a fluid sample.Type: GrantFiled: November 22, 2011Date of Patent: January 3, 2017Assignee: Ascensia Diabetes Care Holdings AGInventor: Jing Lin
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Patent number: 9534245Abstract: A method and device for controlling a fermenting process, wherein the method comprises collecting samples of biological cells from a fermenting tank, obtaining the state information of the current biological cells based on the collected samples, comparing the state information of the current biological cells with preset target status information to obtain the difference between the state information of the current biological cells and preset target state information, and controlling the feed rate of nutritional solution into the fermenting tank. Real time control of biological fermenting process is thus accomplished based on the state information of the biological cells during fermentation, and the consistency during fermentation is improved. Complicated mathematical modeling and man-made data analysis are not required for the method and device.Type: GrantFiled: April 23, 2008Date of Patent: January 3, 2017Assignee: Siemens AktiengesellschaftInventors: Kurt Dirk Bettenhausen, Shun Jie Fan, Herbert Grieb, Jun Feng Jiang, Hongwei Yang, Yue Zhuo
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Patent number: 9534246Abstract: The invention provides methods for the rapid identification and selection of cell lines suitable for biopharmaceuticals production, which do no utilize animal derived components.Type: GrantFiled: June 29, 2011Date of Patent: January 3, 2017Assignee: Glaxo Group LimitedInventors: Ekaterini Kotsopoulou, Richard Priest, Mark Uden
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Patent number: 9534247Abstract: The present invention relates to an apparatus for partitioning a sampling region on a solid surface and a method for collecting a microbial sample from a solid surface, in which an optimal sampling region is partitioned on a solid surface using a light source without bringing the solid surface into direct contact with a ruler or a partitioning tool. With the apparatus and method, a solid surface can be prevented from being contaminated during partitioning process, accuracy of sampling operation can be increased, partitioning operation can be continuously performed in a simple manner, and cost of sampling operation can be reduced.Type: GrantFiled: June 26, 2013Date of Patent: January 3, 2017Assignee: Sanigen Co., Ltd.Inventors: Jeong Woong Park, Dong Jin Woo, Seong Bin Im, Sang Woo Kim
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Patent number: 9534248Abstract: The present disclosure encompasses embodiments of nucleic acid minicircle vectors most advantageous for the detection of tumor cells. In particular, the minicircles of the disclosure incorporate a tumor-specific promoter operably linked to a nucleotide sequence desired to be selectively expressed in a tumor cell or a tissue comprising a population of tumor cells. In embodiments of the disclosure, the minicircle vectors comprise a tumor-specific promoter operably linked to a nucleotide sequence encoding a polypeptide useful as a reporter. Accordingly, when expressed by a recipient tumor cell, the reporter may be detectable, thereby providing information such as a visual image of the tumor cell and/or its location in a tissue of the subject human or non-human animal.Type: GrantFiled: September 9, 2014Date of Patent: January 3, 2017Inventors: Sanjiv S. Gambhir, John A. Ronald
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Patent number: 9534249Abstract: This document provides methods and materials related to assessing prostate cancer in mammals. For example, this document provides nucleic acids and polypeptides that can be analyzed to determine whether a male mammal having prostate cancer is susceptible to a good or poor outcome.Type: GrantFiled: November 30, 2012Date of Patent: January 3, 2017Assignee: Mayo Foundation for Medical Education and ResearchInventors: George Vasmatzis, John C. Cheville, Farhad Kosari, Cemile D. Savci-Heijink, Eric W. Klee, Jan Marie A. Willard, Lori S. Tillmans
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Patent number: 9534250Abstract: The present invention relates to a sensing device with a surface having at least one individual sensing region, wherein each sensing region includes a plurality of binding elements anchored on the surface for binding different specific analytes of interest, at least one of the analyte of interest and its matching binding element having a label for detecting said binding. The present invention further relates to a method of manufacturing such a sensing device.Type: GrantFiled: April 27, 2011Date of Patent: January 3, 2017Assignee: NXP B.V.Inventors: David Van Steenwinckel, Filip Frederix
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Patent number: 9534251Abstract: The present invention relates, e.g., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising contacting the two DNA molecules in a reaction mixture with (a) a non-processive 5? exonuclease; (b) a single stranded DNA binding protein (SSB) which accelerates nucleic acid annealing; (c) a non strand-displacing DNA polymerase; and (d) a ligase, under conditions effective to join the two DNA molecules to form an intact double stranded DNA molecule, in which a single copy of the region of sequence identity is retained. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes.Type: GrantFiled: March 30, 2010Date of Patent: January 3, 2017Assignee: Synthetic Genomics, Inc.Inventors: Lei Young, Hamilton O. Smith, Daniel Glenn Gibson
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Patent number: 9534252Abstract: The present invention relates to the fields of biotechnology and molecular biology. In particular, the present invention relates to the construction and use of nucleic acid molecules comprising cloning sites which differ in nucleotide sequence. In particular embodiments, the present invention relates to nucleic acid molecules which contain recombination sites with different primer binding sites. These different primer binding sites may be used to sequence different ends of nucleic acid segments located between the two recombination sites.Type: GrantFiled: September 14, 2012Date of Patent: January 3, 2017Assignee: Life Technologies CorporationInventors: Jonathan Chesnut, Louis Leong
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Patent number: 9534253Abstract: A mechanism is provided for ratcheting a double strand molecule. The double strand molecule is driven into a Y-channel of a membrane by a first voltage pulse. The Y-channel includes a stem and branches, and the branches are connected to the stem at a junction. The double strand molecule is slowed at the junction of the Y-channel based on the first voltage pulse being weaker than a force required to break a base pair of the double strand molecule. The double strand molecule is split into a first single strand and a second single strand by driving the double strand molecule into the junction of the Y-channel at a second voltage pulse.Type: GrantFiled: February 6, 2015Date of Patent: January 3, 2017Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATIONInventors: Binquan Luan, Ruhong Zhou
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Patent number: 9534254Abstract: The method of the invention comprises the stratification of a cancer patient population into various cancer therapy groups based on analysis by genomic DNA microarray of multiple gene amplifications or deletions present or absent in the diseased tissue of each patient. In particular, the invention involves patient stratification into one of at least four cancer therapy groups based on the microarray analysis of gene amplification or gene deletion at multiple chromosome locations.Type: GrantFiled: March 31, 2000Date of Patent: January 3, 2017Assignee: Abbott Molecular Inc.Inventor: Steven A. Seelig
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Patent number: 9534255Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).Type: GrantFiled: December 17, 2009Date of Patent: January 3, 2017Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Caifu Chen, Ruoying Tan
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Patent number: 9534256Abstract: The present invention provides a method of identifying a subject as having an increased risk of having or developing aggressive prostate cancer, comprising detecting in the subject the presence of various polymorphisms associated with an increased risk of having or developing aggressive prostate cancer.Type: GrantFiled: January 6, 2012Date of Patent: January 3, 2017Assignees: Wake Forest University Health Sciences, The Johns Hopkins UniversityInventors: Jianfeng Xu, William B. Isaacs, Henrik Grönberg
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Patent number: 9534257Abstract: The present invention relates to methods and systems for single molecule based nucleic acid amplification and subsequent detection of nucleic acid molecules, and particularly to the determination of SNPs, mutations, and to the diagnosis of diseases associated with the changes of these nucleic acid molecules.Type: GrantFiled: December 6, 2013Date of Patent: January 3, 2017Assignee: LIFEOS GENOMICS CORPORATIONInventor: Timothy Z. Liu
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Patent number: 9534258Abstract: The invention relates to prognostic markers and prognostic signatures, and compositions and methods for determining the prognosis of cancer in a patient, particularly for melanoma. Specifically, the invention relates to the use of genetic and protein markers for the prediction of the risk of progression of a cancer, such as melanoma, based on markers and signatures of markers. In various aspects, the invention provides methods, compositions, kits, and devices based on prognostic cancer markers, specifically melanoma prognostic markers, to aid in the prognosis and treatment of cancer.Type: GrantFiled: August 29, 2014Date of Patent: January 3, 2017Assignee: PACIFIC EDGE LIMITEDInventors: Michael Alan Black, Jonathan Cebon, Parry John Guilford, Thomas John