Abstract: A composition contains an additive for assisting with the regeneration of the PF in the form of an organic dispersion of iron particles and a detergent including a polyester quaternary ammonium salt.

Abstract: A fuel composition for a direct fuel injected diesel engine, a method for improving performance of fuel injectors and a method for cleaning fuel injectors for a diesel engine. The fuel composition includes a major amount of fuel and a minor, effective amount of a quaternary ammonium salt having a thermogravimetric analysis (TGA) weight loss of greater than 50 wt. % at 350° C. The amount of quaternary ammonium salt present in the fuel is sufficient to improve performance of the direct fuel injected diesel engine having combusted the composition compared to the performance of such engine having combusted a fuel composition that does not contain the quaternary ammonium salt.

Abstract: A fuel additive composition, fuel comprising said additive, and methods of use thereof are provided. The fuel additive composition comprises a synergistic combination of a hydrocarbyl-substituted succinimide dispersant and a compound of the following formula: and tautomers and enantiomers thereof, wherein R3 is a hydrocarbyl group having a number average molecular weight ranging from about 100 to about 5000, and wherein the weight ratio of (a) to (b) ranges from about 5:1 to about 1:5. The fuel additive composition is present in fuel in an amount sufficient to improve the conductivity properties of the fuel.

Abstract: Colloidal coal-in-water slurries having nano-particles of coal creating a pseudo-fluid. The colloidal coal-in-water slurry generally includes from about fifty to about seventy two weight percent of coal, with about 20 to about 80 percent of the coal having a particle size of about one micron or less with a mode particle size of about 250 nanometers. The coal-in-water slurry can also include a surfactant system containing one surfactant or mixtures of two or more surfactants, or mixtures of one or more surfactants and an inorganic or organic salt. The coal-in-water slurry can be used in low NOx burner applications as the main fuel and/or the reburn fuel, in gasification processes as the input fuel either alone, or in combination with organic materials, in gas turbine applications, and in diesel engine applications.

Abstract: A diesel fuel and additive mixture is useful for reducing particulate matter emissions while improving or at least not aggravating oxidative stability during combustion. The additive includes at least one compound having a general formula selected from the group consisting of: and combinations thereof, wherein: R is a saturated or unsaturated hydrocarbon having from about 1 to about 6 carbons. The additive is effective in diesel at concentrations as low as from about 50 to about 1000 ppm by weight.

Abstract: New compositions of matter that provide lubricant properties when cured into a coating on a substrate. The compositions are low molecular weight structured polysilicate compositions that have high silanol contents. The polysilicates are combined with a condensation catalyst and allowed to cure on the appropriate substrate. The cured coating has lubricant properties.

Abstract: The present invention provides a sheet that has excellent lubricity and good touch feeling under wet conditions and suffers from little drop of the lubricity even if the sheet is used repeatedly. In the sheet of the present invention, a layer of modified polyalkylene oxide obtainable by reacting a polyalkylene oxide compound, a diol compound, and a diisocyanate compound together is directly formed on a surface of a substrate.

Abstract: A lubricating composition and method of making the same are provided. The lubricating composition comprises a lubricant fluid, water, and carbon nanoparticles comprising nanodiamonds. The method comprises mixing the lubricating composition under high shear followed by ultrasonication.

Abstract: A lubricant composition comprising lubricating oil and a compound containing phosphorus and an amine of formula I: wherein R1 is a hydrocarbon group and at least one of R1 is selected from: —(C1-C20)alkyl, —(C1-C20)alkenyl, —(C5-C20)cycloalkyl; R is selected from: —(C4-C20)alkyl, —(C4-C20)alkenyl, —(C5-C20)cycloalkyl; and wherein R2, R3 and R4 are independently selected from: —H, —(C6-C36)alkyl, —(C6-C36)alkenyl, —(C6-C36)cycloalkyl, wherein at least one of R2, R3 and R4 is not H; X is O or S; Y1 and Y2 are selected from S and O; m is 1 or 2, n is 0 or 1, p is 1 or 2, provided that m+n+p=3 and q=0.8 to 1.2*p, wherein the compound provides at least 48 ppm of phosphorus and a molar ratio phosphorus to nitrogen (P/N) in the range of about 0.40 to about 1.50.

Abstract: A lubricating oil composition contains a lubricating base oil and components (A) to (D): (A) a primary amine; (B) a tertiary amine; (C) at least one of metal sulfonate, metal phenate and metal salicylate; and (D) at least one of acid phosphate and acid phosphite.

Abstract: A compression-ignited engine lubricant and a method for reducing engine wear. The lubricant including, (a) a base oil; (b) oleamide; (c) zinc dihydrocarbyl dithiophosphate; and (d) a functionalized dispersant. The functionalized dispersant is made by reacting together (i) a hydrocarbyl-dicarboxylic acid or anhydride, (ii) a polyamine, (iii) a dicarboxyl-containing fused aromatic compound or anhydride thereof, and optionally, (iv) a non-aromatic dicarboxylic acid or anhydride, wherein the hydrocarbyl group of the hydrocarbyl-dicarboxylic acid or anhydride has a number average molecular weight of greater than 1800 Daltons as determined by gel permeation chromatography.

Abstract: The invention provides a grease composition for use in an image forming apparatus, containing a thickener, a base oil and a lubricity improver, wherein the thickener is a lithium soap, the base oil is a synthetic hydrocarbon oil, the lubricity improver is a polyethylene oxide wax, and the image forming apparatus is equipped with a sliding member and a member for holding the sliding member which is driven to slide through the holding member via the grease composition, one of the sliding member or the holding member for the sliding member having a sliding surface formed from a metal, and the other having a sliding surface formed from a resin.

Abstract: The use of poly(oxyethylene)-poly(oxybutylene) block copolymers in pharmaceutical compositions useful for modifying the surfaces of contact lenses and other medical devices is disclosed. The present invention is based in-part on a discovery that this class of compounds is particularly efficient in wetting hydrophobic surfaces, such as the surfaces of silicone hydrogel contact lenses and other types of ophthalmic lenses. Such compounds are also useful for cleaning purposes. The use of the compounds as surfactants in various types of compositions for treating contact lenses therefore represents a preferred embodiment of the present invention.

Abstract: The present disclosure relates to compositions and systems comprising fit retention polymers and methods of making and using the same. Methods of using such compositions including contacting a fabric with the fabric care composition are also disclosed.

Abstract: A stable, liquid bleach composition is disclosed. The composition comprises a peroxide-based bleaching agent, water, a metal catalyst, and a polyol. The polyol is a secondary solvent that promote peroxide stability in the presence of a catalyst.

Abstract: Caustic-free detergent compositions are provided. Detergent compositions including an aminocarboxylate, water conditioning agent, non-caustic source of alkalinity and water beneficially do not require the use of additional surfactants and/or polymers to provide suitable detergency and prevent scale build-up on treated surfaces. The detergent compositions are used with a sanitizer to employ the caustic-free detergent compositions are particularly suitable for use as low temperature ware wash detergents that beneficially reduce scale build-up. Methods of employing the caustic-free detergent compositions are also provided.

Abstract: A laundry stain and soil pretreatment sheet including a water soluble or water dispersible carrier layer, preferably polyvinyl alcohol, a removable separator layer, and a layer of cleaning agent composition therebetween. The separator layer is removed, the composition layer is adhered to a stain on clothing, and the clothing is laundered. The carrier layer dissolves or disperses during the laundering. The separator layer can be water soluble or water dispersible. Depressions can be provided in the carrier layer to hold the layer of cleaning agent composition. Without the separator layer, a sheet of the carrier layer can be folded over to hold the cleaning agent composition.

Abstract: Disclosed herein is a cell culture apparatus that can achieve appropriate culture conditions. The cell culture apparatus (1) includes: a cylindrical culture vessel (10) that holds a culture liquid containing cells; a supporting column (20) that stands upright in a center of an inner surface of a bottom (12) in the culture vessel; and a stirring device (30) that includes an attaching portion (32) that is attached to an upper portion of the supporting column so as to be rotatable relative to the supporting column and a stirring blade (34) whose upper portion is fixed to the attaching portion so as to rotate around the supporting column.

Abstract: A bioreactor or culture vessel for incubation of one or more cell cultures, tissue biopsies, cell clusters, tissue-like structures, “prototissues” or similar samples is described. The bioreactor comprises an incubation chamber, a liquid reservoir and conduction means separated from each other by semipermeable membranes. Specifically, the invention provides a humidity chamber design which simplifies the construction of the bioreactor.

Abstract: A microreactor with a reaction chamber comprising two sub-chambers with reconfigurable volumes, an input conduit with input valve, an un-filtered output conduit with first output valve, and a filtered output conduit with a second output valve is used to perform a method to control a different dilution rate for cells and molecules in continuous culture bioprocesses. In preferred embodiments flow control is achieved using the difference in volume of the two sub-chambers.

Abstract: A method for collecting cells that have been cultured by using a cell culture kit in which, at least, a culture container for cultivating cells, a cell collection container for collecting cells after cultivation and a waste liquid container for collecting a culture medium after cultivation are linked to one another through a conduit is provided. The method includes transferring a culture supernatant that has been cultured from the culture container to the waste liquid container, transferring a concentrated suspension of cells from the culture container to the cell collection container, returning part of the culture supernatant discharged in the waste liquid container to the culture container and allowing cells remaining in the culture container to be suspended in a suspension of cells, and transferring again a suspension of cells in which remaining cells are suspended from the culture container to the cell collection container.

Abstract: The present invention comprises the discovery and development of an effective cryoprotectant composition, without containing skim milk or any other animal-derived compounds, to achieve long-term stability of freeze-dried lactic acid bacteria (LAB), at different temperatures, whereby the retention of viability of the freeze-dried LAB after 6 months of storage, preferably after 9 months of storage, more preferably after 12 months of storage is more than 50%. The invention is in the field of producing freeze dried bacteria, in particular Lactic acid bacteria. More in particular, the invention relates to the use of a novel combination of cryoprotectants for increasing the viability of bacteria after freeze drying, improving the texture of the lyofilized cake for easy grinding and improving the long term stability of the freeze dried bacteria at different temperature conditions. The invention further relates to such freeze dried bacteria for use in food industry or in human or animal health applications.

Abstract: The present invention relates to culturing cells utilizing a matrix of microfibrillated thermoplastic polymeric materials. More specifically, the present invention relates to a method of culturing cells. In addition, the invention relates to a biaxially oriented microfibrillated article for culturing cells dispersed in a cell culture medium. The matrix of thermoplastic polymeric materials for culturing cells of this invention finds use in tissue engineering and wound healing applications.

Abstract: A method of generating a population of corneal epithelial cells is disclosed. The method comprises culturing human pluripotent stem cells in corneal fibroblast-conditioned medium on a solid surface comprising an extracellular matrix component thereby generating the population of corneal epithelial cells. Isolated cell populations and corneal tissues are also disclosed, as well as uses thereof.

Abstract: A method is described for producing a microfluidic device (19), which comprises the phases of producing a three-dimensional template (15) of geometry equal to the channelings that is desired to obtain in the device; inserting the template in the desired position into a mold (16), keeping it suspended by at least one of its end; coating said template by immersion in (or deposition of) a material in the liquid phase (or dissolved or dispersed in a solvent) capable of solidifying by means of a chemical reaction or physical transformation, forming a material constituting the body of the final device; and selectively removing the three-dimensional template. In a variant of the method, useful for the production of scaffolds to be inserted into the human body, a porogenic material is added to the liquid precursor or to the precursor solution, such that the material of the solid matrix is characterized by a continuous structure of pores into which it is possible to insert live cells.

Abstract: Provided herein are isolated neural stem cells. Also provided are methods for treatment of neurodegenerative diseases using suitable preparations comprising the isolated neural stem cells.

Abstract: A method for determining the effect of a plurality of culture conditions on a cell, comprising the steps of: a) providing a first set of groups of cell units each comprising one or more cells, and exposing said groups to desired culture conditions; (b) pooling two or more of said groups to form at least one second pool; (c) subdividing the second pool to create a further set of groups of cell units; (d) exposing said further groups to desired culture conditions; (e) optionally, repeating steps (b)-(d) iteratively as required; and (f) optionally assessing the effect on a given cell unit of the culture conditions to which it has been exposed.

Abstract: An endometrial polyp stem cell is disclosed in the present invention. The endometrial polyp stem cell is isolated from an endometrial polyp tissue, expresses vimentin, CD13, CD29, CD44, and CD90, and has no expression of CD1q, CD3, CD34, and CD45.

Abstract: The present invention provides a neural stem cell having increased passage ability and a method for manufacturing a neural stem cell having increased passage ability. A neural stem cell in which the N-type calcium channel gene is knocked out or the influx of Ca2+ via the N-type calcium channel is substantially absent can be passaged for at least 4 generations and maintains the differentiation potential into a nerve cell even after passage for 4 generations.

Abstract: The invention is directed to methods for reducing the number of apoptotic cell deaths in a population of cells undergoing excessive cellular apoptosis. The invention is also directed to methods for preventing apoptotic cell death in a population of cells at risk for developing excessive cellular apoptosis. In particular, the invention is directed to methods for reducing or preventing excessive cellular apoptosis comprising exposing cells exhibiting or at risk for developing excessive cellular apoptosis to a cellular factor-containing composition called Amnion-derived Cellular Cytokine Solution (referred to herein as ACCS), which is obtained from the culturing of Amnion-derived Multipotent Progenitor (AMP) cells, or AMP cells.

Abstract: Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein.

Abstract: The invention generally regards methods for providing hematopoietic cells and precursors of hematopoietic cells from a variety of cell sources, such as pluripotent stem cells or somatic cells. Also provided are therapeutic compositions including the provided hematopoietic cells and precursors of hematopoietic cells, and methods of using such for the treatment of subjects.

Abstract: A method of obtaining beta-islet cells from pancreases of rabbits and a composition for transplantation (xenografts or xenotransplants) of beta-islet cells isolated and cultured from rabbit pancreases to promote natural insulin production among people with diabetes.

Abstract: Methods for producing reassortant viruses are provided wherein the transcription and/or translation of the hemagglutinin and/or neuraminidase genes are suppressed.

Abstract: The object of the present invention is to provide Humulus lupulus-derived monoterpene glycosyltransferase and a method for producing a monoterpene glycoside by means of this enzyme. The present invention provides Humulus lupulus-derived monoterpene glycosyltransferase and a method for producing a monoterpene glycoside by means of this enzyme. The present invention provides a transformant transformed with a gene for Humulus lupulus-derived monoterpene glycosyltransferase and a method for producing such a transformant.

Abstract: Methods and kits for DNA repair are provided. The methods and kits described herein repair multiple types of DNA damage. The kit may include a plurality of enzymes to repair a greater variety of lesions than any single enzyme is capable of repairing. Repair of damaged DNA may include releasing damaged bases from the DNA strand, nicking the DNA at the damaged sites, translating the nicks via 5?-3? exonuclease activity, and sealing the nicks. The enzymes employed in the repair process may then be heat-inactivated, thereby obviating a purification process. The repaired DNA may then be analyzed using a variety of DNA analysis methods.

Abstract: The present disclosure relates to methods of treating diseases states such as lysosomal storage diseases and/or neurodegenerative diseases. Also disclosed are one or more compositions that may be useful for one or more of the disclosed methods, including compositions that may comprise acid ?-glucosidase (GCase) protein comprising one or more mutations, peptides of acid ?-glucosidase, and DNA vectors and cell lines related to acid ?-glucosidase peptides or proteins.

Abstract: A cold-adapted protease derived from Pseudoalteromonas arctica PAMC 21717 or a recombinant cold-adapted protease obtained by expressing a gene encoding the cold-adapted protease in E. coli is described, and more particularly, a crystal of a protease exhibiting activity at low temperatures, a method for crystallizing the protease, a method for preparing the protease, a recombinant microorganism that expresses the protease, a method for preparing the recombinant microorganism, a method for preparing the recombinant protease and the use of the cold-adapted protease. The cold-adapted protease exhibits high activity at low temperatures, and securely maintains its enzymatic activity even in the presence of high pH, various metal ions and surfactants. Thus, it is useful in various industrial applications.

Abstract: The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilisate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.

Abstract: Provided are compositions comprising newly identified protein fragments of aminoacyl-tRNA synthetases, polynucleotides that encode them and complements thereof, related agents, and methods of use thereof in diagnostic, drug discovery, research, and therapeutic applications.

Abstract: The disclosed methods in the application relates to making an adapter-tagged restriction library. The library or libraries created with the disclosed methods avoid the self-ligation of the restriction fragments. The application further disclosed methods of using the library or libraries to measure the methylation level of a genome, or comparing methylation levels among two or more genomes. The measurement of the global methylation levels can be achieved with quantitative PCR method by measuring the number of restriction fragments in the libraries. It also can be used for next generation Sequencing (NGS), Copy Number Variation (CNV), restriction site mutation, endogenous gene jumping, and exogenous DNA insertion, somatic hypermutation, gene knockout/knock in etc.

Abstract: Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reaction site with one or more reactants, and b) reacted enzymatically at the priming site with one or more tag(s) identifying the reactant(s).

Abstract: An object of the present invention is to provide molecules that bind to a target molecule in a pH dependent manner and a screening method for selecting such molecules. Provided is a screening method for selecting peptides that bind to a target molecule at a first pH and do not bind thereto at a second pH, including a step of preparing a peptide library in which each peptide contains at least one special amino acid that undergoes a pH-dependent change in the charge of the side chain thereof, a step of bringing the peptide library into contact with the target molecule and incubating them under the first pH condition and selecting peptides that bind to the target molecule, and a step of selecting, from the peptides which have bound to the target molecule, peptides that do not bind to the target molecule under the second pH condition.

Abstract: The present invention relates to the selective inhibition of protein expression of CAG repeat-related disease proteins such as Huntingtin Disease Protein and Ataxin-3 using double-stranded RNAs and nucleic acid analogs. Chemically-modified RNAs having at least one mismatch as compared to the target CAG repeat sequence are specifically contemplated.

Abstract: The invention relates to RNAi agents, e.g., double-stranded RNAi agents, targeting the Serpina1 gene, and methods of using such RNAi agents to inhibit expression of Serpina1 and methods of treating subjects having a Serpina1 associated disease, such as a liver disorder.

Abstract: Disclosed herein are antisense compounds and methods for decreasing apo(a) to treat, prevent, or ameliorate diseases, disorders or conditions related to apo(a) or Lp(a). Certain diseases, disorders or conditions related to apo(a) or Lp(a) include inflammatory, cardiovascular and/or metabolic diseases, disorders or conditions. The antisense compounds disclosed herein can be used to treat such diseases, disorders or conditions in an individual in need thereof.

Abstract: Described herein are compositions and methods for the inhibition of miR-122 activity. The compositions have certain nucleoside modifications that yield potent inhibitors of miR-122 activity. The compounds may comprise conjugates to facilitate delivery to the liver. The compositions may be administered to subjects infected with hepatitis C virus, as a treatment for hepatitis C virus and related conditions.

Abstract: Provided herein are RNAi agents for inhibition of Chikungunya virus. The present disclosure provides RNAi agents for inhibition of Chikungunya virus, particularly by targeting the E2 gene and nsP1 gene or both of the Chikungunya virus; the RNAi agents comprising of the entire nucleotide sequence set forth is SEQ ID 1 or SEQ ID 5 or combination thereof; or comprising of 15 or more contiguous nucleotides as set forth is SEQ ID 1 or SEQ ID 5 or combination thereof along with the addition nucleotides from the contiguous region of the E2 and nsP1 target gene. The invention further provides a RNAi composition for reducing the E2 protein and nsP1 protein level of Chikungunya virus and inhibition of Chikungunya virus replication. The combination of RNAi agents provides an excellent therapeutic composition for treatment of Chikungunya virus infection.

Abstract: Embodiments of the present invention relates to the incorporation and use of a regulated genetic suicide mechanism for use in the improved purification of biologics, including adjunct use in various eubacterial minicell production and purification methodologies. Described herein are high-yield eubacterial minicell-producing strains with genetic modifications that comprise a regulated genetic suicide mechanism that irreparably destroys the parent cell chromosome such that live parental cells in a culture can be functionally eliminated at any time during the course of a minicell production and purification run. Embodiments of the present invention also describe methods useful in the elimination of live parental cells during the production of other cell-based biologics.

Abstract: Disclosed herein are systems and methods for producing recombinant proteins utilizing mutant E. coli strains containing expression vectors carrying nucleic acids encoding the proteins, and secretory signal sequences to direct the secretion of the proteins to the culture medium. Host cells transformed with the expression vectors are also provided.