Abstract: In order to conduct gene expression analysis of a number of genes in a number of cells, it has been necessary to separate cells, extract genes therefrom, amplify nucleic acids, and perform sequence analysis. However, separation of cells imposes damages on the cells, and it requires the use of an expensive system. Gene expression analysis in each cell can be carried out with high accuracy by arranging a pair of structures comprising a cell trapping section and a nucleic acid trapping section in a vertical direction to extract individual genes in relevant cells, synthesizing cDNA in the nucleic acid trapping section, amplifying nucleic acids, and analyzing the sequences using a next-generation sequencer.

Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method includes, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.

Abstract: The present invention concerns bioactive renal cell populations, renal cell constructs, and methods of making and using the same.

Abstract: The present invention relates to an oligonucleotide structure and a method for preparing the same and, more particularly, to an oligonucleotide structure in which a polymer compound is linked to an oligonucleotide via a covalent bond to improve in vivo stability of the oligonucleotide and cellular delivery efficiency of the oligonucleotide; and to a method for preparing the same.

Abstract: The invention relates to RNAi agents, e.g., double-stranded RNAi agents, targeting the Serpina1 gene, and methods of using such RNAi agents to inhibit expression of Serpina1 and methods of treating subjects having a Serpina1 associated disease, such as a liver disorder.

Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.

Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Z. mays genotype, Z. mays c.v. B104.

Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Z. mays genotype, Z. mays c.v. Hi-II.

Abstract: The present disclosure provides compositions and methods for regulating expression of transcribable polynucleotides in plant cells, plant tissues, and plants. Compositions include regulatory polynucleotide molecules capable of providing expression in plant tissues and plants. Methods for expressing polynucleotides in a plant cell, plant tissue, or plants using the regulatory polynucleotide molecules disclosed herein are also provided.

Abstract: Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that negatively regulates alkaloid biosynthesis, such as nicotine biosynthesis.

Abstract: The present invention relates to vaccines that are made in transgenic soybeans for use in humans, animals of agricultural importance, pets, and wildlife. These vaccines are used as vaccines against viral, bacterial, fungal, parasitic or prion related diseases, cancer antigens, toxins, and autologous or self proteins. The transgenic soybeans of the instant invention also can be used for inducing tolerance to allergens or tolerance to autoimmune antigens, wherein an individual shows hypersensitivity to said allergen or has developed autoimmunity to autologous or self proteins, respectively. The invention also relates to prophylatically treating individuals and/or populations prior to showing hypersensitivity to allergens. Other aspects of the invention include using the transgenic soybeans as an oral contraceptive, and the expression of protein adjuvants in transgenic soybeans.

Abstract: The present invention concerns a transgenic plant of the species Solanum tuberosum with a resistance to an oomycete of the genus Phytophthora, transgenic parts of such a plant, a method for its manufacture and to a composition for external application to plants. On the one hand, nucleotide sequences in accordance with SEQ ID NOS: 1-43 are provided from Phytophthora in a host plant-induced gene silencing strategy in potato plants; on the other hand, a fungicide for plant treatment is provided.

Abstract: The present disclosure relates to nucleic acid vaccine compositions and methods for preventing or treating pathological conditions, such as cancer or infectious disease. Further, the disclosure provides methods for more efficient production of antigens via mRNA containing one or more non-conventional start codons to promote multiplex initiation of translation in eukaryotic cells.

Abstract: A method for synthesizing long DNA constructs from oligonucleotide precursors directly within a microfluidic device uses several oligonucleotides at once. A precursor mix containing at least two oligonucleotide precursors with at least partial base complementarity is introduced into an input of a microfluidic chip and at least one cycle of at least one gene synthesis protocol is applied to fabricate a DNA construct containing the sequence of at least two oligonucleotide precursors. A method for the synthesis of a modified DNA construct includes electroporating at least one oligonucleotide encoding for at least one point mutation and having homology with at least one DNA region of a target cell into the target cell and incorporating the oligonucleotide into the target cell DNA through the action of recombination protein beta or a recombination protein beta functional homolog.

Abstract: The present invention provides a method of producing hydrogen from a waste material, comprising steps of fermenting 100 a fermentation mixture comprising the waste material in a reactor 1 with a headspace 1a under anaerobic conditions, removing 200 hydrogen from a gas from the headspace 1a during fermentation to produce a hydrogen gas and a remainder gas and recirculating 300 at least a portion of the remainder gas back to the headspace 1a. An apparatus for producing hydrogen and recirculating at least a portion of the remainder gas to the headspace 1a is also provided.

Abstract: The subject of the present invention is a process for synthesizing bifunctional hydrocarbon-based compounds from biomass, comprising a step of fermentation of the biomass and a step of oxidation of the intermediate compounds resulting from the fermentation step.

Abstract: A method for producing lysophosphatidylethanolamine 18:1 includes extracting phospholipids including mainly phosphatidylethanolamine from a microorganism of Pseudomonas sp. and treating the extracted phospholipids with phospholipase A2. An alternative method for producing lysophosphatidylethanolamine 18:1 includes treating a microorganism of Pseudomonas sp. directly with phospholipase A2. The lysophosphatidylethanolamine 18:1 can be used as a plant vaccine material for preventing the plants from injuries caused by pathogen infections and/or environmental stresses and accelerating the recovery of plants injured by pathogen infections and/or environmental stresses, and can also be used as a composition for enhancing fruit ripening (color and sweetness) and storage properties, and as it can be used for an application in plant tissues, food products, pharmaceuticals, cosmetics, and agricultural use, it would be very advantageously used in related industries.

Abstract: Provided is a separatome-based recombinant peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome, such as that of E. coli, yeast, Bacillus subtilis or other prokaryotes, insect cells, mammalian cells, etc. The separatome-based protein expression and purification platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both. Moreover, the separatome-based protein expression and purification platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies leading to efficient product purification.

Abstract: The present invention relates to a microorganism comprising a gene for coding an enzyme involved in producing retinoid and a method for producing retinoid by using the same, and more specifically, to: a microorganism capable of mass-producing retinoid at a remarkable efficiency by comprising a gene for coding an enzyme involved in producing retinoid; and a method for producing retinoid by using the same.

Abstract: Methods for ultrasonically imaging a heterogeneous 3D-cell population without physically probing are provided. The method can comprises: pulsing ultrasound waves having a wave frequency of about 10 MHz to about 2 GHz through a lens rod, wherein the lens rod focuses the ultrasound waves onto the cell population via a concave lens-head (e.g., comprising sapphire), and wherein the cell population is positioned on the reflective surface such that the ultrasound waves are reflected back to the lens-head; receiving the reflected waves through the lens head and the lens rod at a signal receiver; scanning the lens-head across multiple points in the x,y plane; and at each point in the x,y plane, moving the lens-head in a z-direction such that a signal is received at multiple intervals in the z-direction for each point in the x,y plane.

Abstract: The present invention relates to the field of enzymes. More specifically, the present invention provides an inducible reconstitution and real-time quantitative kinetic system for the study of intramembrane enzymes. In a specific embodiment, a method of screening for modulators of an intramembrane protease comprises the steps of (a) contacting in a mixture the protease and a substrate with a lipid under acidic or basic conditions to form a membrane comprising the lipid bilayer, protease and the substrate; (b) contacting a test agent with the membrane mixture; (c) adjusting the pH to physiological conditions; (d) assaying substrate cleavage by the protease; and (e) comparing the assayed substrate cleavage to a reference that does not include the test agent, wherein an increase or a decrease of substrate cleavage by the protease relative to the reference identifies the test agent as a modulator of the intramembrane protease.

Abstract: The present invention relates to methods and products for the localized or spatial detection of nucleic acid in a tissue sample and in particular to a method for localized detection of nucleic acid in a tissue sample comprising: (a) providing an array comprising a substrate on which multiple species of capture probes are directly or indirectly immobilized such that each species occupies a distinct position on the array and is oriented to have a free 3? end to enable said probe to function as a primer for a primer extension or ligation reaction, wherein each species of said capture probe comprises a nucleic acid molecule with 5? to 3?: (i) a positional domain that corresponds to the position of the capture probe on the array, and (ii) a capture domain; (b) contacting said array with a tissue sample such that the position of a capture probe on the array may be correlated with a position in the tissue sample and allowing nucleic acid of the tissue sample to hybridize to the capture domain in said capture probes;

Abstract: A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present, counting the number of fractions with an amplified target nucleic acid, and determining the quantity of target nucleic acid in the sample.

Abstract: Described herein are multiplexed RNA, including miRNA, PCR-based assays.

Abstract: Methods for performing analytical reactions and compositions for use in such methods, where the methods have reduced signal levels deriving from non-specific adsorption of detected reagents to other components of the analytical method, e.g., other reagents, solid phase components, vessels, etc.

Abstract: Embodiments of the invention include a method for fabricating a semiconductor device, the resulting structure, and a method for using the resulting structure. A substrate is provided. A hard mask layer is patterned over at least a portion of the substrate. Regions of the substrate not protected by the hard mask are doped to form a source region and a drain region. The hard mask layer is removed. A dielectric layer is deposited on the substrate. An insulative layer is deposited on the dielectric layer. A nano-channel is created by etching a portion of the insulative layer which passes over the source region and the drain region.

Abstract: In the field of the next generation DNA sequencer, a method for integrating very high sensitive FET sensors having side gates and nanopores as devices used for identifying four kinds of base and for mapping the base sequence of DNA without using reagents, and a semiconductor device having selection transistors and amplifier transistors respectively corresponding to the FET sensors having side gates and nanopores respectively so as to be able to read the variation of a detection current based on the differences among the charges of the four kinds of base without deteriorating the detection sensitivity of the FET sensor, are presented.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

Abstract: The present invention relates to compositions and methods for detection, analysis, and treatment of nucleic acids. In particular, the present invention relates to compositions and methods for generating and using hybridization probes.

Abstract: The present invention relates to the discovery that the modulation of particular microRNAs can be employed to inhibit a mesenchymal transition that, in certain instances, correlates with resistance to therapy and recurrence as the corresponding cells acquire properties of stem cells as they start undergoing this transition, as well as with invasiveness, e.g., invasion of certain cells of primary tumors into adjacent connective tissue during the initial phase of metastasis. Accordingly, the identification inhibitors of this mechanism, such as inhibitors of certain microRNAs, disclosed herein, can be used for inhibiting the mesenchymal transition to reduce the invasive nature of certain cells of primary cancerous tumors and, in certain instances, to prevent the recurrence of cancer by inhibiting the induction of stem cell-like features in certain cancer cells.

Abstract: The present application relates to K-ras mutations, to polynucleotides encoding mutant K-ras polypeptides, and to methods of identifying K-ras mutations. The present application also relates to methods of diagnosing cancer; and methods and kits for predicting the usefulness of anti-EGFr specific binding agents in the treatment of tumors.

Abstract: Using an RT-PCR platform, detection of gene transcripts highly expressed in prostate tissue and expressed in peripheral blood mononuclear cells (PBMC) from patients with mCRPC can provide a more reliable and robust prediction of poor overall survival than that of CTC enumeration in mCRPC. Disclosed is the identification of five genes, KLK3, KLK2, HOXB13, GHRL2 and FOXA1, the detection of two (2) or more transcripts of which predicts overall poor survival. The test is performed on blood samples that have been collected in collection tubes that stabilize intracellular RNA, require minimal on-site processing and can be easily stored and shipped for subsequent extraction of total RNA from whole blood for RT-PCR.

Abstract: Provided herein is technology for gastrointestinal neoplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of gastrointestinal neoplasm, and classifying the site location of such a gastrointestinal neoplasm (e.g., a colorectal region, a pancreaticobiliary region, a gastroesophageal region).

Abstract: The present invention provides novel methods and compositions for the diagnosis, prognosis and treatment of disorders by examining samples containing microvesicles and miRs therein.

Abstract: This invention provides a means for screening for lactic acid bacteria having immunoregulatory functions in a simple and rapid manner. This invention provides a method for screening for or producing lactic acid bacteria having immunoregulatory functions comprising determining the number of the test lactic acid bacteria bound to the uromodulin (Umod) protein, lactic acid bacteria obtained by such method, and an immunoregulatory composition comprising such lactic acid bacteria.

Abstract: The present invention relates to primers and probes that can be used in various assays to detect a new strain of Chlamydia trachomatis. The invention further provides for the simultaneous detection of other diseases, especially Neisseria gonorrhoeae.

Abstract: Disclosed is a method for determining the presence of Mycobacterium tuberculosis complex nucleic acids in a test sample. In particular, regions of the IS6110 preferential locus (ipl) 3?-flanking region of the Mycobacterium tuberculosis complex genome are amplified and detected. In addition, oligonucleotides that can be used as primers to amplify the ipl 3?-flanking region and probe oligonucleotides are described.

Abstract: The invention provides cotton event MON 88701, and plants, plant cells, seeds, plant parts, and commodity products comprising event MON 88701. The invention also provides polynucleotides specific for event MON 88701 and plants, plant cells, seeds, plant parts, and commodity products comprising polynucleotides specific for event MON 88701. The invention also provides methods related to event MON 88701.

Abstract: The invention relates to a method of identifying a specific yeast species in patient tissue or body fluid. The method comprises the steps of extracting and recovering DNA of the yeast species from the patient tissue or body fluid, amplifying the DNA, hybridizing a probe to the DNA to specifically identify the yeast species, and specifically identifying the yeast species. The invention also relates to a method of identifying a yeast mycotoxin in patient tissue or body fluid. The method comprises the steps of extracting and recovering the yeast mycotoxin from the patient tissue or body fluid, contacting the yeast mycotoxin with an antibody directed against the yeast mycotoxin, and identifying the yeast myocotoxin. Both of these methods can he used to determine if a patient is at risk for or has developed a disease state related to a yeast infection, and to develop an effective treatment regimen for the patient.

Abstract: A controller for a compost system configured to identify an error condition is disclosed. The controller comprises at least one control output. The control output is configured to measure a load current of the at least one control output and generate a load value. The at least one control output is in communication with a compost device and is configured to control an environmental condition of a compost chamber of the compost system. The controller is operable to compare the load value to a predetermined value to determine an error condition of the compost system.

Abstract: The present invention provides a steel sheet with chemical components including, by mass %, 0.18-0.35% of C, 1.0%-3.0% of Mn, 0.01%-1.0% of Si, 0.001%-0.02% of P, 0.0005%-0.01% of S, 0.001%-0.01% of N, 0.01%-1.0% of Al, 0.005%-0.2% of Ti, 0.0002%-0.005% of B, and 0.002%-2.0% of Cr, and the balance of Fe and inevitable impurities, wherein: by volume %, a fraction of the ferrite is 50% or more, and a fraction of a non-recrystallized ferrite is 30% or less; and Cr?/CrM is 2 or less, where Cr? is a concentration of Cr subjected to solid solution in iron carbide and CrM is a concentration of Cr subjected to solid solution in a base material, or Mn0/MnM is 10 or less, where Mn0 is a concentration of Mn subjected to solid solution in an iron carbide, and MnM is a concentration of Mn subjected to solid solution in a base material.

Abstract: Disclosed are a steel pipe having a three-layer structure and a manufacturing method thereof. The steel pipe includes a three-layer structure of bainite and martensite, which are formed by high-frequency induction heating thereby improving toughness to enhance crash performance of a vehicle. The steel pipe includes a bainite structure layer, a bainite and martensite dual-phase structure layer, and a martensite structure layer.

Abstract: This ferritic stainless steel sheet contains, by mass %: C: 0.02% or less, N: 0.02% or less, Si: 0.05% to 0.80%, Mn: 0.05% to 1.00%, P: 0.04% or less, S: 0.01% or less, Cr: 12% to 20%, Cu: 0.80% to 1.50%; Ni: 1.0% or less, Mo: 0.01% to 2.00%, Nb: 0.30% to 1.00%, Ti: 0.01% to less than 0.25%, Al: 0.003% to 0.46%, V: 0.01% to less than 0.15%, and B: 0.0002% to 0.0050%, with a remainder of Fe and inevitable impurities, wherein the following formulae (1) and (2) are satisfied, and an average Cu concentration in an area from a surface to a depth of 200 nm is 3.00% or less, in the case of Mn<0.65%, 1.44×Si?Mn?0.05?0??(1), and in the case of Mn?0.65%, 1.10×Si+Mn?1.19?0??(2).

Abstract: Provided is a method for producing a surface-treated steel sheet for battery containers including: a first process of forming an iron-nickel alloy plating layer on at least one side of a steel sheet; a second process of forming a nickel plating layer on the iron-nickel alloy plating layer; and a third process of performing a thermal treatment after forming the nickel plating layer to form an iron-nickel alloy layer having an outermost surface, at which a content ratio of Fe atoms is 12 to 55% by atom, on an outermost layer by thermal diffusion. The invention makes it possible to provide the method for producing the surface-treated steel sheet for battery containers that can suppress the elution of iron inside the battery when being used for a battery container, whereby the service life of the battery can be extended and battery characteristics such as discharge characteristics can be improved.

Abstract: Methods for producing steel components with well-adhering metallic coatings that provide protection from corrosion offer flexibility in processing qualities. In one example, a flat steel product comprising a steel material that is hardenable by quenching in a hot forming operation and that has a yield point of 150-1100 MPa and a tensile strength of 300-1200 MPa may be coated electrolytically with a thin zinc layer. From the flat steel product, a blank may then be obtained that is heated directly to at least 800° C. and then formed into the steel component. Alternatively, the blank may initially be formed into the steel component and then heated to at least 800° C. Either way, the steel component may then be hardened by sufficiently rapid cooling from a sufficiently high temperature.

Abstract: In a solvent extraction settler arrangement the outlet box comprises an inner tube arranged vertically inside a shaft, the inner tube being spaced from the side wall of the shaft to define an intermediate space between the inner tube and the shaft. The inner tube has an inner space and an opening at the lower part of the inner tube adjacent the bottom to form a flow path for the heavy solution phase to flow to the inner space. The shaft comprises a second outlet which is separate in relation to the discharge outlet and above the level of the discharge outlet. The second outlet opens through the side wall to the intermediate space at a location adjacent to the upper end of the shaft and at the level of said layer of entrained light solution phase for discharging said layer of entrained light solution phase from the intermediate space.

Abstract: A method of extracting rare metals from ore including: providing an aqueous acid-leached ore slurry; adding an organic extractive solvent to the aqueous acid-leached ore slurry; mixing an organic extractive solvent with the aqueous acid-leached ore slurry to form a mixture; and separating the mixture into at least an aqueous phase and a solvent phase. The aqueous acid-leached ore slurry may have a viscosity of less than 400 centipoise, a Newtonian or near Newtonian rheology, and a pH of less than 4.0. The organic extractive solvent may comprise an extractant, a solvent, and a modifier. Separation of the aqueous acid-leached ore slurry/organic extractive solvent mixture may result in an emulsion phase, a crud, or both in addition to the aqueous phase and the solvent phase. The emulsion phase, the crud or both may be further treated by adding a low-carbon-number alcohol.

Abstract: A soft dilute copper alloy material includes 2 mass ppm to 12 mass ppm of sulfur, more than 2 mass ppm and not more than 30 mass ppm of oxygen, 4 mass ppm to 55 mass ppm of Ti, and a balance including copper. An average crystal grain size is not more than 20 ?m in a surface layer up to a depth of 50 ?m from a surface. The average crystal grain size in the surface layer is less than the average crystal grain size in an inner portion located more interiorly than the surface layer.

Abstract: A method for making an article is disclosed. The method involves first generating a digital model of the article. The digital model is inputted into an additive manufacturing apparatus comprising an energy source. The additive manufacturing apparatus applies energy from the energy source to successively applied incremental quantities of a powder to fuse the powder to form the article corresponding to the digital model. The powder includes an aluminum alloy having 90.15-95.80 wt. % aluminum, 3.00-4.50 wt. % silicon, 0.70-1.50 wt. % magnesium, 0.50-1.00 wt. % manganese, 0-0.50 wt. % iron, 0-0.10 wt. % copper, 0-0.50 wt. % titanium, 0-0.20 wt. % boron, 0-1.50 wt. % nickel, and 0-0.05 wt. % other alloying elements, based on the total weight of the aluminum alloy.

Abstract: A spheroidal graphite cast iron for a component of an engine exhaust system includes carbon ranging from about 3.0 wt % to about 3.4 wt %, silicon ranging from about 4.2 wt % to about 4.5 wt %, manganese ranging from about 0.1 wt % to about 0.3 wt %, sulfur ranging from about 0.002 wt % to about 0.01 wt %, phosphorous in a range equal to or less than about 0.05 wt %, magnesium ranging from about 0.035 wt % to about 0.055 wt %, molybdenum ranging from about 0.9 wt % to about 1.2 wt %, nickel ranging from about 0.2 wt % to about 0.5 wt %, vanadium ranging from about 0.4 wt % to about 0.6 wt %, niobium ranging from about 0.1 wt % to about 0.4 wt %, cerium ranging from about 0.005 wt % to about 0.01 wt %, aluminum ranging from about 0.003 wt % to about 0.007 wt %, and a remainder of iron.