Abstract: A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.

Abstract: Methods and compositions are provided for improved nucleic acid amplification assays.

Abstract: An integrated semiconductor device for manipulating and processing bio-entity samples and methods are described. The device includes a lower substrate, at least one optical signal conduit disposed on the lower substrate, at least one cap bonding pad disposed on the lower substrate, a cap configured to form a capped area, and disposed on the at least one cap bonding pad, a fluidic channel, wherein a first side of the fluidic channel is formed on the lower substrate and a second side of the fluidic channel is formed on the cap, a photosensor array coupled to sensor control circuitry, and logic circuitry coupled to the fluidic control circuitry, and the sensor control circuitry.

Abstract: This invention provides substrates for use in various applications, including single-molecule analytical reactions. Methods for propagating optical energy within a substrate are provided. Devices comprising waveguide substrates and dielectric omnidirectional reflectors are provided. Waveguide substrates with improved uniformity of optical energy intensity across one or more waveguides and enhanced waveguide illumination efficiency within an analytic detection region of the arrays are provided.

Abstract: Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences.

Abstract: The present invention relates to a DNA sequence showing difference between the female and male individuals of Litopenaeus vannamei and its corresponding acquired method. The method comprises: respectively conducting high-through put sequencing on the mixing pools of female individuals and male individuals in by high-through put sequencing platform; conducting bioinformatic analysis on the sequencing results to screen out sequences showing significant difference between the mixing pools of female and male individuals; verifying the obtained sex difference sequence in individuals from different sources; and finally, obtaining a probe sequence for female and male identification of L. vannamei, so that the genetic sex of this species can be accurately identified using the sequences. The method of the present invention has the characteristics of high efficiency, accuracy and reliability, and possess broad application potential in the early sex identification and sex control research of prawns.

Abstract: The present disclosure relates to the identification of single nucleotide polymorphisms (SNPs) in the Gamma genomic block in the central region of the major histocompatibility complex (MHC) that can be used for matching transplant donors and recipients and determining disease susceptibility.

Abstract: The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-J? IGK rearrangement, a VK/intron-Kde IGK rearrangement, a V?-J? IGL rearrangement, a V?-J? TCRB rearrangement, a D?-J? TCRB rearrangement, a V?-J? TCRG rearrangement, a V?-J? TCRD rearrangement, a D?-D? TCRD rearrangement, a D?-J? TCRD rearrangement, a V?-D? TCRD rearrangement, or a translocation selected from t(11;14)(BCL1-IGH) and t(14;18)(BCL2-IGH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention.

Abstract: The invention provides compositions and methods for identifying autism and autism spectrum disorders in humans. The invention also includes compositions and methods for identifying unique gene expression profiles in children with regressive autism spectrum disorder (ASD) and ileocolitis.

Abstract: The present invention relates to the field of biomarkers. More specifically, the present invention relates to the use of biomarkers to predict suicide ideation and/or suicide attempt. In one embodiment, a method for predicting suicide ideation and/or attempt by a subject comprises the steps of (a) measuring the DNA methylation level of a CpG dinucleotide in the 3? untranslated region of SKA2; (b) identifying the genotype at a SNP within the 3? UTR of SKA2, and (c) predicting suicide ideation and/or attempt by the subject using a prediction algorithm.

Abstract: Methods of determining relapse in subjects having relapsing-remitting multiple sclerosis (RRMS) and predicting their response to glatiramer acetate (GA) using the biomarkers SIRT1, RGC-32, FasL and IL-21 are presented.

Abstract: This disclosure relates to methods and kits, systems for screening, diagnosing and prognosing a disease, disorder, or physiological state based upon temporal measurements and analysis of gene expression in a subject.

Abstract: A method for quantifying the expression level of human WT1 mRNA conveniently, in a short period of time, and with high sensitivity is provided. The method can be used for diagnosing cancer, such as leukemia and solid cancer, or for determining when to perform bone marrow transplantation. The method is for quantifying the expression level of human WT1 mRNA by one-step RT-PCR and comprises simultaneously subjecting the human WT1 mRNA and a housekeeping gene (mRNA) to reverse transcription and extension reactions carried out sequentially in the same vessel.

Abstract: Methods for selecting whether to administer an anti-angiogenic therapeutic agent to a subject include steps of measuring the expression levels of one or more biomarkers selected from Table 2 or Table 3 in a sample from the subject; assessing from the expression levels of the one or more biomarkers whether the sample from the subject is positive or negative for a biomarker signature, wherein if the sample is positive for the biomarker signature an anti-angiogenic therapeutic agent is contraindicated. Related prognostic methods and treatment methods are also provided. The invention is particularly applicable in ovarian and colorectal cancers.

Abstract: A method of quantifying multiple target nucleic acid sequences in a sample includes generating from a sample at least a plurality of first template molecules and at least a plurality of second template molecules. At least part of said first and at least part of said second template molecules are randomly distributed into individual reaction sites. A cluster of nucleic acid amplicons of said first template molecule and at least a cluster of nucleic acid amplicons of said second template molecule are generated by clonal amplification or replication. The ID codes of all said nucleic acid amplicon clusters are identified. The quantity of at least said first and second target nucleic acid sequences in said sample is quantified by statistical analysis of respective positive numbers of identified unique ID codes of first and second target nucleic acid sequences.

Abstract: Embodiments of the invention include methods and/or compositions for analysis of samples for C. difficile infection to determine whether or not an individual is at risk for having recurrent C. difficile infection or a CDI misdiagnosis. Methods include characterization of microflora composition from the gut, wherein alterations of the microflora gut composition are indicative of recurrence of infection. Methods include analysis of nucleic acids from the gut, such as 16S rRNA as being identifying of a particular bacteria in the analysis of bacterial populations of the gut.

Abstract: Methods for detecting micro-organisms and/or biological substances in a fluid are provided.

Abstract: Methods for obtaining genetic material from plant embryos while preserving their viability as well as methods for performing a molecular analysis of plant embryos, particularly with small quantities of genetic material by collecting shed cellular material from one or more plant embryos; obtaining DNA from the shed cellular material; performing a molecular analysis of the DNA; and germinating at least one of the one or more plant embryos. A further extension includes determining whether to germinate and grow the embryo or to discard the embryo based on its genotype as part of a breeding process. Also provided are methods of genotyping embryos using embryo shed cellular material contained in or on agar and methods of analyzing plant embryonic tissue derived from microspores.

Abstract: The present invention provides novel and non-obvious improvements to dried blood spot testing for HIV-1 viral load useful for diagnosis and monitoring treatment progression.

Abstract: Disclosed herein is a dispersing agent composition essentially comprising polyalkylene polyhydric compound, an organic acid and a non-ionic surfactant. The dispersing composition finds application in leather processing industry for tanning without adding any water or any other medium. It finds tremendous application potential in the tanning industry to ensure eco-benign leather processing that does not add to environmental pollution as no effluent is generated. The invention is therefore envisaged to play a crucial role in enhancing the economic and environmental benefits associated with tanning industry. The disclosure also relates to a process for preparing the composition and also the process of eco-benign tanning using the said composition.

Abstract: A hot-dip galvanized steel sheet includes a steel sheet and a hot-dip galvanized layer arranged on the steel sheet, in which the Si content and the Al content by mass % of components of the steel sheet satisfy a relationship 0.5<Si+Al<1.0, and a metallographic structure of the steel sheet satisfies a relationship of {(n2)2/3×d2}/{(n1)2/3×d1}×ln(H2/H1)<0.3 when the n1 is the number of a MnS of a surface portion of the steel sheet, the d1 ?m is an average equivalent circle diameter of the MnS in the surface portion of the steel sheet, the H1 GPa is a hardness of a martensite of the surface portion of the steel sheet, the n2 is the number of the MnS of a center portion of the steel sheet, the d2 ?m is an average equivalent circle diameter of the MnS in the center portion of the steel sheet, and the H2 GPa is the hardness of the martensite of the center portion of the steel sheet.

Abstract: An H-section steel has a predetermined chemical composition, in which a Mg-containing oxide having an equivalent circle diameter of 0.005 ?m to 0.5 ?m is contained at a total number density of 100 pieces/mm2 to 5000 pieces/mm2, a thickness of a flange is 100 mm to 150 mm, at a strength evaluation portion which is at a ? position from a surface of the flange in a length direction and at a ¼ position from the surface in a thickness direction, a fraction of bainite in a steel structure is 80% or more, and the average prior austenite grain size is 70 ?m or more, and at a toughness evaluation portion which is at a ½ position from the surface of the flange in the length direction and at a ¾ position from the surface of the flange in the thickness direction, the average prior austenite grain size in a steel structure is 200 ?m or less.

Abstract: Methods for producing flat steel product with a yield strength of at least 700 MPa and an at least 70% by volume bainitic microstructure may comprise several steps. For example, one method may involve smelting a steel melt including in percent by weight 0.05-0.08% C, 0.015-0.500% Si, 1.60-2.00% Mn, 0.025% P, up to 0.010% S, 0.020-0.050% Al, up to 0.006% N, 0.40% Cr, 0.060-0.070% Nb, 0.0005-0.0025% B, 0.090-0.130% Ti, unavoidable impurities, and Fe. The may further involve casting the melt to give a slab, reheating the slab, rough-rolling the slab, hot finish-rolling the rough-rolled slab, cooling the hot-finish-rolled flat steel product within ten seconds of hot finish-rolling, and coiling the hot-finish-rolled flat steel product.

Abstract: A tool arrangement is disclosed for integration in a production line for producing a hot formed component from a blank which is produced from a hot forming steel strip. The tool arrangement includes a housing, which delimits an interior of the tool arrangement relative to a surroundings of the tool arrangement, a tempering station for tempering the blank and a hot forming station for hot forming the blank. The tempering station and the hot forming station are jointly arranged in the interior.

Abstract: An earth-boring tool includes a tool body, at least one cutting element, and a retaining member comprising a shape memory material (e.g., alloy, polymer, etc.) located between a surface of the tool body and a surface of the cutting element. The shape memory material is configured to transform, responsive to application of a stimulus, from a first solid phase to a second solid phase. The retaining member comprises the shape memory material in the second solid phase, and at least partially retains the at least one cutting element adjacent the tool body. The shape memory material may be trained in a first phase to a first shape, and trained in a second phase to a second shape. The retaining member may be at least partially within a cavity in the first phase, then transformed to the second phase to apply a force securing the cutting element to the tool body.

Abstract: A laser shock peening method and device for a bottom surface of a tenon groove of an aircraft blade. During the laser shock peening process, according to geometric characteristics of the bottom surface of a tenon groove, a circular facula of a laser beam is changed into a strip-shaped facula, at the same time as a flow-guiding injection device and a water pumping device are respectively arranged at two end surfaces of the bottom surface of the tenon groove to ensure the stability of a water confinement layer.

Abstract: A bioleaching and solvent extraction process with selective recovery of copper and zinc from polymetallic sulphide concentrates is described, comprising a bioleaching and ferric ion reducing process and a copper and zinc solvent extraction process. The bioleaching and ferric ion reducing process comprises a concentrates pulp conditioning step; a bioleaching step, wherein once the pulp is conditioned it is subjected to a bioleaching process using a plurality of bioreactors of the stirred-tank type with an air injection and diffusion system, which allows handling of a pulp density higher than 15%; a step of solid separation from a solution rich in metallic ions from the bioleaching step; and, a ferric ion reduction step, wherein the pulp from the previous step is subjected to a transformation step of ferric ions into ferrous ions.

Abstract: Disclosed is a method of recovering rare earth, aluminum and silicon from rare earth-containing aluminum-silicon scrap. The method comprises: S1, acid-leaching the rare earth-containing aluminum-silicon scrap with an inorganic acid aqueous solution to obtain a silicon-rich slag and acid leached solution containing rare earth and aluminum element; S2, adding an alkaline substance into the acid leached solution containing the rare earth and aluminum element and controlling a PH value of the acid leaching solution between 3.5 to 5.2, performing a solid-liquid separation to obtain a aluminum hydroxide-containing precipitate and a rare earth-containing solution filter; S3, reacting the aluminum hydroxide containing precipitate with sodium hydroxide to obtain sodium metaaluminate solution and aluminum-silicon slag, and preparing a rare earth compound product with the rare earth-containing filtrate.

Abstract: The invention provides a continuous method for extracting transition metal, the method comprising: supplying a spent generator liquor comprising transition metal in highly alkaline solution; mixing the liquor with acid thereby generating a solution, wherein the transition metal resides within the solution; combining the solution with an organic liquid comprising tributyl phosphate or other neutral extractant to extract the transition metal within the organic liquid; washing the extracted transition metal in the organic liquid with acid so as to remove non-transition-metal salts from the organic liquid phase; and stripping the washed transition metal loadedorganic liquid phase with hydroxide, water or complexing agent to remove the transition metal from the organic phase.

Abstract: The present invention provides a zinc alloy with improved alloy characteristics such as fluidity, castability, mechanical properties, corrosion resistance and elongation, and a preparation method therefor. The method for preparing the zinc alloy, according to one aspect of the present invention, comprising the steps of: providing zinc and a magnesium master alloy including a calcium-based compound; and forming a molten metal in which the magnesium master alloy and the zinc are melted; and casting the molten metal. The zinc alloy, according to another aspect of the present invention, includes a zinc base and the calcium-based compound present in the zinc base, wherein magnesium is applied to the zinc base.

Abstract: A process for producing a metal foam. The process includes mechanically working a metallic powder such that oxide particles are finely dispersed within a metallic matrix of the metallic particles that make up the metallic powder. The mechanically worked metallic powder is then annealed in a reducing atmosphere, which in turn reduces the oxide particles within the metallic particles such that H2O gas molecules (steam) are formed within the metallic matrix and intraparticle porosity is produced.

Abstract: A nickel-based superalloy composition includes from about 5 to about 7 wt % aluminum, from about 4 to about 8 wt % tantalum, from about 3 to about 8 wt % chromium, from about 3 to about 7 wt % tungsten, from 1 to about 5 wt % molybdenum, from 1.5 to about 5 wt % rhenium, from 5 to about 14 wt % cobalt, from about 0 to about 1 wt % hafnium, from about 0.01 to about 0.03 wt % carbon, from about 0.002 to about 0.006 wt % boron, and balance nickel and incidental impurities. The composition may exhibit a sustained peak low cycle fatigue life at 1800° F./45 ksi of at least about 4000 cycles. The nickel-based superalloy composition may be used in single-crystal or directionally solidified superalloy articles, such as a blade, nozzle, a shroud, a splash plate, and a combustor of a gas turbine engine.

Abstract: The high alloy for oil well according to the present embodiment consists of, in mass %, C: 0.03% or less, Si: 1.0% or less, Mn: 0.05 to 1.5%, P: 0.03% or less, S: 0.03% or less, Ni: 26.0 to 40.0%, Cr: 22.0 to 30.0%, Mo: 0.01% or more to less than 5.0%, Cu: 0.1 to 3.0%, Al: 0.001 to 0.30%, N: more than 0.05% to 0.30% or less, O: 0.010% or less, and Ag: 0.005 to 1.0%, wherein the alloy satisfies the following Formula (1) and (2), wherein the high alloy for oil well has yield strength of 758 MPa or more: 5×Cu+(1000×Ag)2?40??(1) Cu+6×Ag?500×(Ca+Mg+REM)?3.5??(2) where, each element symbol in each Formula is substituted by the content (in mass %) of each element.

Abstract: An iron-based powder for powder metallurgy includes an iron-based powder and a composite oxide powder, and the composite oxide contains, by mass, from 15% to 30% Si, from 9% to 18% Al, from 3% to 6% B, from 0.5% to 3% Mg, from 2% to 6% Ca, from 0.01% to 1% Sr, and from 45% to 55% O.

Abstract: A molten bath-based direct smelting process comprises controlling the process conditions in a direct smelting vessel so that molten slag in a molten bath of metal and slag in the vessel has a viscosity in a range of 0.5-5 poise when the slag temperature is in the range of 1400-1550° C. in the molten bath in the vessel.

Abstract: Mixed magnetic powders for making a magnetic core or body is disclosed, wherein the mixed magnetic powders comprises a first magnetic powder and a second magnetic powder, each of the first magnetic powder and the second magnetic powder being made of a soft magnetic material, wherein the average particle diameter of the first magnetic powder is greater than that of the second magnetic powder, and each of the first magnetic powder and the second magnetic powder has a pre-configured particle size distribution for increasing the density of the magnetic body.

Abstract: Provided is a lean duplex stainless steel used in industrial facilities including fresh water, pulp and paper making, chemical and construction facilities. The lean duplex stainless steel comprises, in weight percentage (%), C: over 0 to 0.06 or less, Si: over 0 to 1.5% or less, Mn: over 0 to 2% or less, Cr: 19 to 23%, Ni: 1.8 to 3.5%, Mo: 0.5 to 1.0%, Cu: 0.3 to 1.0%, N: 0.16 to 0.30%, Al: 0.003 to 0.05%, B: 0.001 to 0.005% and Ca: 0.001 to 0.01%. In the stainless steel, the content of O of the stainless steel is 0.01% or less. The stainless steel comprises Fe and other unavoidable impurities as remnants.

Abstract: A steel alloy is disclosed that provides a unique combination of strength, toughness, and fatigue life. The steel alloy has the following composition in weight percent: C about 0.15 to about 0.30 Mn about 1.7 to about 2.3 Si about 0.7 to about 1.1 Cr about 1.85 to about 2.35 Ni about 0.5 to about 0.9 Mo+½W about 0.1 to about 0.3 Cu about 0.3 to about 0.7 V+ 5/9×Nb about 0.2 to about 0.5. The balance of the alloy is iron, usual impurities, and residual amounts of other elements added during melting for deoxidizing and/or desulfurizing the alloy. A hardened and tempered steel article made from the alloy is also disclosed.

Abstract: One embodiment provides a structure, comprising: a display; at least one structural component disposed over a portion of the display, wherein the at least on structural component comprises at least one amorphous alloy; and wherein a portion of the display is foldable.

Abstract: The disclosure is directed to Zr and Hf bearing alloys that are capable of forming a metallic glass, and more particularly metallic glass rods with diameters at least 1 mm and as large as 5 mm or larger. The disclosure is further directed to Zr and Hf bearing alloys that demonstrate a favorable combination of glass forming ability, strength, toughness, bending ductility, and/or corrosion resistance.

Abstract: An aluminum alloy fin material for heat exchanger use having a 35 to 50 ?m thickness, a small springback at the time of corrugation, a suitable strength before brazing enabling easy fin formation, a high strength after brazing, and excellent erosion resistance, self corrosion resistance, and sacrificial anodic effect and a method of production of the same are provided. A fin material containing, by mass %, Si: 0.9 to 1.2%, Fe: 0.8 to 1.1%, Mn: 1.1 to 1.4%, and Zn: 0.9 to 1.1%, further limiting the impurity Mg to 0.05% or less, Cu to 0.03% or less, and ([Si]+[Fe]+2[Mn])/3 to 1.4% to 1.6%, and having a balance of unavoidable impurities and Al. A method of production prescribing hot rolling, cold rolling, intermediate annealing, and final cold rolling.

Abstract: A magnesium alloy includes Mg, 1 to 12 wt % of Li, 1 to 10 wt % of Al and 0.2 to 3 wt % of Zn. The magnesium alloy has a microstructure which includes a nanoscale reinforcement phase, wherein the nanoscale reinforcement phase is a Li—Al compound.

Abstract: An aluminum bronze alloy containing 7.0-10.0% by weight Al; 3.0-6.0% by weight Fe; 3.0-5.0% by weight Zn; 3.0-5.0% by weight Ni; 0.5-1.5% by weight Sn; ?0.2% by weight Si; ?0.1% by weight Pb; and the remainder Cu in addition to unavoidable impurities. Also described is an aluminum bronze product having such an alloy composition, and a method for producing such a product from an aluminum bronze alloy.

Abstract: A nickel-base alloy is disclosed that has the following weight percent composition. C about 0.005 to about 0.06 Cr about 13 to about 17 Fe about 4 to about 20 Mo about 3 to about 9 W up to about 8 Co up to about 12 Al about 1 to about 3 Ti about 0.6 to about 3 Nb up to about 5.5 B about 0.001 to about 0.012 Mg about 0.0010 to about 0.0020 Zr about 0.01 to about 0.08 Si up to about 0.7 P up to about 0.05 and the balance is nickel, usual impurities, and minor amounts of other elements as residuals from alloying additions during melting. The alloy provides a combination of high strength, good creep resistance, and good resistance to crack growth. A method of heat treating a nickel base superalloy to improve the tensile ductility of the alloy is also disclosed. An article of manufacture made from the nickel base superalloy described herein is also disclosed.

Abstract: A composition and a coating structure applying with the same are provided. The composition includes 3 wt % to less than 15 wt % of Al, 10 wt % to less than 30 wt % of Cr, higher than 0 wt % to 15 wt % of O, higher than 0 wt % to 15 wt % of Y, and the remainder being at least one of Co or Ni.

Abstract: In a process for carbonitriding metallic components (1), provision is made of at least one carburization phase (C1, C2), in which the metallic component (1) is carburized with a carbon-donating gas. In addition, provision is made of a nitriding phase (C1, C2, C3), which is assigned to the carburization phase (D1, D2) and precedes the carburization phase (D1, D2). Here, in the nitriding phase, the metallic component (1) is nitrided by means of a nitrogen-donating gas at least superficially at at least one surface region (4) of the metallic component (1). It is thereby possible to avoid an excessive concentration of carbon, and therefore component failure is prevented and it is possible to dispense with costly remachining steps, e.g. grinding.

Abstract: There is provided a roughened copper foil which can significantly improve adhesion to an insulating resin and reliability (e.g., hygroscopic heat resistance). The roughened copper foil of the present invention has at least one roughened surface having fine irregularities composed of acicular crystals, wherein the entire surface of the acicular crystals is composed of a mixed phase of Cu metal and Cu2O.

Abstract: A crucible structure including a crucible body and a crucible cover. The crucible body includes a crucible bottom wall and a crucible side wall. One end of the crucible side wall is connected to the crucible bottom wall, and the other end of the crucible side wall is provided with the crucible cover. An included angle between the crucible cover and an axial direction of the crucible is an acute angle. The crucible cover is provided with an opening structure.

Abstract: A racetrack-shaped apparatus for generating a magnetic field on a target surface for magnetron sputtering, comprising on a magnetic base (a) a vertically magnetized center permanent magnet arranged straight; (b) vertically magnetized peripheral permanent magnets surrounding the center permanent magnet; (c) vertically magnetized first intermediate permanent magnets, horizontally magnetized second intermediate permanent magnets and vertically magnetized third intermediate permanent magnets arranged on both sides of the center permanent magnet; and (d) vertically magnetized fourth intermediate permanent magnets arranged separately from both longitudinal ends of the center permanent magnet; each second intermediate permanent magnet being arranged with one magnetic pole opposing a near-target side surface portion of each first intermediate permanent magnet.

Abstract: A sapphire structure including an ion-implanted, anti-reflective layer and a method of forming an ion-implanted, anti-reflective layer within a sapphire component is disclosed. The method includes forming an ion-implanted layer in a sapphire material at a first depth, and annealing the sapphire material to a second depth. The second depth may be greater than or equal to the first depth. The ion-implanted layer may have a first index of refraction, and the sapphire material may have a second index of refraction different from the first index of refraction.