Abstract: The present invention relates to methods for treating prostate cancer patients. It is based, at least in part, on the discovery that approximately 90% of men carrying at least one of the following fusion genes: TRMT11-GRIK2, SLC45A2-AMACR, MTOR-TP53BP1, LRRC59-FLJ60017, TMEM135-CCDC67 and CCNH-05orf30 experienced prostate cancer recurrence, metastases and/or prostate cancer-specific death after radical prostatectomy (each examples of “progressive prostate cancer”), while these outcomes occurred in only 36% of men not carrying any of these fusion genes. It is also based, at least in part, on the discovery that a genome editing technique that specifically targets a fusion gene can induce cell death in a cancer cell that carries the fusion gene.

Abstract: The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of unsaturated C6-C24 fatty alcohols, aldehydes, and acetates which may be useful as insect pheromones, fragrances, flavors, and polymer intermediates. The C6-C24 fatty alcohols, aldehydes, and acetates described herein may be used as substrates for metathesis reactions to expand the repertoire of target compounds and pheromones. The application further relates to recombinant microorganisms co-expressing a pheromone pathway and a pathway for the production of a toxic protein, peptide, oligonucleotide, or small molecule suitable for use in an attract-and-kill pest control approach. Also provided are methods of producing unsaturated C6-C24 fatty alcohols, aldehydes, and acetates using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally one or more of the product alcohols, aldehydes, or acetates.

Abstract: The invention relates to improved processes of producing ethanol from starch-containing material wherein saccharification and/or fermentation is done at a temperature below the initial gelatinization temperature in the presence of glucoamylase and alpha-amylase, and optionally a protease and/or a cellulolytic enzyme composition; wherein the fermenting organism is a Saccharomyces yeast strain providing a higher ethanol yield boost and lower glycerol production compared to ETHANOL RED™ under the same fermentation conditions. The invention also relates to Saccharomyces yeast strains and derivatives thereof, as well as compositions comprising such yeast strains, suitable for use in a process of the invention.

Abstract: The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.

Abstract: The present invention provides biological oils and methods and uses thereof. The biological oils are preferably produced by heterotrophic fermentation of one or more microorganisms using cellulose-containing feedstock as a main source of carbon. The present invention also provides methods of producing lipid-based biofuels and food, nutritional, and pharmaceutical products using the biological oils.

Abstract: The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

Abstract: Provided are processes for treating crop kernels which comprising the steps of a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of a polypeptide having protease activity.

Abstract: Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan.

Abstract: The present invention provides a method for producing a multimeric protein composed of a monomeric protein, wherein the monomeric protein is obtained by fusing a protein having an immunoglobulin fold structure to a protein that can serve as a subunit structure, the method including the steps of: (A) preparing the monomeric protein having an insoluble granular form in cells of a microorganism; (B) solubilizing the monomeric protein prepared in step (A) with an aqueous solution containing lauroyl-L-Glu; (C) diluting a solution obtained in step (B) in a buffer containing arginine hydrochloride to lower a concentration of lauroyl-L-Glu; and (D) replacing a solvent of a solution obtained in step (C) with a buffer using gel filtration chromatography or the like.

Abstract: The present invention relates to a method or process for controlling, inhibiting or reducing protein fucosylation in a eukaryote and/or eukaryotic protein expression system. Said method comprises carrying out the protein expression and/or post-translational modification in the presence of an elevated total concentration of manganese or manganese ions.

Abstract: The invention relates to an isolated, genetically modified, living non-mammal organism, having increased HMG-CoA-reductase activity compared to the wild type, and having reduced C24-methyltransferase and/or delta22-desaturase activity compared to the wild type. The invention is characterized in that the organism has increased dehydrocholesterol-delta70-reductase activity compared to the wild type. The invention further relates to different uses of such an organism, to a test kit comprising such an organism, and to a membrane extract of such an organism.

Abstract: The subject of the present invention is a whole-cell catalysis process for converting substrates of cytochrome P450 monooxygenases of eukaryotic origin into valuable biotechnological products. The subject of the present invention is also microorganisms genetically engineered to achieve those biotransformations with high rates and processes to prepare these microorganism strains.

Abstract: Provided is a real-time imaging method of NAPDH-dependent metabolic activity in a cell and a use thereof, and more particularly, an imaging method capable of measuring concentration gradient and change in an intracellular coenzyme using a mutant of metagenome-derived blue fluorescent protein (mBFP) in real-time and an application method thereof. In particular, the method of imaging and quantifying the coenzyme contained in various biological samples may be performed by improving fluorescence in a short time even under anaerobic conditions, without adding a substrate required for coenzyme measurement or conversion without destroying cells, and without consuming the time required for formation of a special structure (fluorophore) for generating fluorescence unlike the existing quantitative systems.

Abstract: Provided is a compound that comprises the structure: where SIG is a signaling molecule and R3 is a formyl, a succinyl, a methyl succinyl, or a myristoyl. Also provided is a kit is provided that comprises the above compound, with instructions for determining the presence of the enzyme. Additionally, a method is provided for determining whether a sample has an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ?-amino of a lysine. Also provided is a method of determining whether a molecule inhibits an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ?-amino of a lysine.

Abstract: Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogs, kits comprising the compounds, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods for making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.

Abstract: Methods and devices for the selective lysis of cells in a sample comprising micro-organisms such as bacteria are provided. The selective lysis is obtained by incubating the sample in a non-ionic detergent under alkaline conditions.

Abstract: A device for processing a biological sample includes a first substrate having a first cavity, a second substrate having a second cavity, a filter element having a plurality of perforations, and an electrically conductive structure. The first cavity forms a first chamber segment of a chamber for accommodating the biological sample. The second cavity forms a second chamber segment of the chamber for accommodating the biological sample. The filter element is formed between the first cavity and the second cavity in order to hold back a plurality of organic cells of the biological sample on the plurality of perforations when the biological sample moves between the first cavity and the second cavity through the filter element. The electrically conductive structure is arranged on the filter element and is configured to cause lysis of the organic cells and/or to duplicate and/or detect defined segments of exposed DNA.

Abstract: Transposon nucleic acids comprising a transposon end sequence and a calibration sequence for DNA sequencing in the transposon end sequence. In one embodiment, the transposon end sequence is a Mu transposon end. A method for the generation of DNA fragmentation library based on a transposition reaction in the presence of a transposon end with the calibration sequence providing facilitated downstream handling of the produced DNA fragments, e.g., in the generation of sequencing templates.

Abstract: This disclosure provides a method comprising: a) clamping the top and bottom strands of a double stranded DNA molecule to produce a duplex in which the top and bottom strands are linked; b) denaturing the duplex to produce a denatured product; and c) renaturing the denatured product in the presence of a labeled oligonucleotide that is complementary to a sequence of nucleotides in the double stranded DNA molecule, thereby producing a D-loop-containing product. Kits for performing the method and products made by the method are also provided.

Abstract: The present invention relates to methods and biomarkers (e.g., gene expression biomarkers) for detection of colorectal cancer in biological samples (e.g., tissue samples, biopsy samples, stool samples, blood samples, plasma samples, serum samples). In some embodiments, methods and biomarkers of the present invention find use in detection of colon cancer and in providing a prognosis to colorectal cancer patients.

Abstract: The present invention provides assay systems and methods for detection of copy number variation at one or more loci and polymorphism detection at one or more loci in a mixed sample from an individual.

Abstract: The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

Abstract: The present invention relates to systems and methods for measuring chromosome structure and gene expression. In one embodiment, the present invention includes an assay for determining the spatial organization of gene expression in the nucleus. The present invention also includes a method based on fluorescence in situ hybridization (FISH) that simultaneously yields information on the physical position and expression of individual genes. By lighting up a large number of targets on a particular chromosome using a bar-coding scheme, the large scale structure of an entire chromosome can be determined.

Abstract: Nucleic acids sequences that can be used for nucleic acid amplification, for example quantitative nucleic acid amplification, are provided herein.

Abstract: Disclosed herein is a method for diagnosing a renal allograft recipient's risk for developing fibrosis of the allograft and allograft loss. The method includes determining the expression levels of certain microRNAs, which have been determined to be predictive of an allograft recipient's risk. Also disclosed herein is a method of treating a renal allograft recipient to inhibit fibrosis of the allograft and allograft loss, as well as kits for use in the methods disclosed herein.

Abstract: The present invention provides assays, methods and systems for selecting an effective therapy for a subset of cancer patients having cancer cells with increased expression of BML and FANCI genes and/or having copy number increase in chromosome location 15q26 in the cancer cells and for treatment of such patients with the effective therapy of cancer patients based on the personalized cancer cell expression profile.

Abstract: A method for detecting cancer in an individual comprising detecting a deletion in the nucleic acid sequence between residues 10743 and 14125 in mitochondrial DNA, obtaining a biological sample from the individual, extracting the mitochondrial DNA (mtDNA) from the sample, quantifying the amount of mtDNA in the sample having a deletion in the nucleic acid sequence between residues 10743 and 14125 of the mtDNA genome, and comparing the amount of mtDNA in the sample having the deletion to at least one known reference sample.

Abstract: The present invention provides a corn plant designated MON88017 and DNA compositions contained therein. Also provided are assays for detecting the presence of the corn plant MON88017 based on a DNA sequence and the use of this DNA sequence as a molecular marker in a DNA detection method.

Abstract: The instant disclosure provides norovirus binding aptamers, compositions comprising such aptamers, and methods of using and producing such aptamers. The aptamers are useful, for example, for detecting the presence of norovirus in test samples, for capturing and/or concentrating norovirus from test samples, for evaluating the efficacy of therapeutic agents in patients diagnosed with a norovirus infection, and for evaluating the efficacy of norovirus vaccines.

Abstract: Methods of evaluating a cellular sample for latent cellular replication competent HIV-1 are provided. Aspects of the methods include contacting a cellular sample with an HIV-1 inducing compound to produce an activated cellular sample; and assessing plasma viral load in the activated cellular sample to evaluate the cellular sample for latent cellular replication competent HIV-1. Also provided are devices and kits that find use in practicing the methods.

Abstract: A method of separating a saccharide from an aqueous product solution of the cellulose hydrolysis process is provided. The aqueous product solution comprises a saccharide and a cellulose swelling agent. The cellulose swelling agent is zinc chloride, magnesium chloride or a combination thereof. The method comprises the following steps in the given order: (a) adding a first tertiary amine and an optional first organic solvent to the aqueous product solution to provide a mixture; (b) performing a solid-liquid separation to obtain a solution from the mixture; and (c) performing a liquid-liquid extraction by adding a second tertiary amine and a second organic solvent to the solution, and then removing the organic phase and collecting the aqueous phase, wherein the first tertiary amine and the second tertiary amine are the same or different, and the first organic solvent and the second organic solvent are the same or different.

Abstract: A method and system are disclosed for treating a press hardened part by induction heating localized areas of the part to have reduced hardness. The method and system monitor an ambient temperature, cycle time, outgoing part property requirements, and outgoing part hardness in local areas. A time value and temperature value are set by a computer system for a plurality of induction heaters. A local area of the part is induction heated to soften the part in localized areas. The hardness of the localized areas is tested after induction heating.

Abstract: A quench system for applying cooling air to one or more hot metallic components that are supported on a component support having a substantially open construction. The quench system includes a housing having sidewalls that define a cooling chamber with peripheral portions proximate the sidewalls and a center portion spaced inwardly from the sidewalls. The quench system also includes a conveyance system that is configured to carry the component support into the center portion of the cooling chamber, as well as a forced air fan that generates a bulk flow of cooling air through the cooling chamber. The quench system further includes a plurality of nozzle baffles extending inwardly from the plurality of sidewalls to define a narrowing region within the housing between the forced air fan and the conveyance system, whereby, during operation of the fan, cooling air flowing through the peripheral portions of the cooling chamber is redirected into the center portion of the cooling chamber.

Abstract: A seamless steel tube has a particular composition and a structure with an average prior ? grain size of 150 ?m or less in an axial cross-section after cold drawing and heat treatment. The structure retards the growth of a fatigue crack. The steel tube has a tensile strength TS of 500 MPa or more and good internal pressure fatigue resistance and is suitable for use as a fuel injection tube under high injection pressures. The composition of the steel tube may further contain at least one of Cu, Ni, Cr, Mo, and B; at least one of Ti, Nb, and V; and/or Ca.

Abstract: The invention relates to the manufacture of a cold-rolled steel sheet having a strength of more than 900 MPa, an elastic limit of more than 700 MPa and distributed elongation of more than 12%, the composition of which includes, the contents being expressed as weight percentages: 0.26%?C?0.45%, 1.0%?Mn?3.0%, 1.0%?Si?3.0%, Al?0.10%, 0%?Cr?1.5%, S?0.005%, P?0.020%, Nb?0.1%, Ti?0.020%, V?0.015%, N?0.01%, the remainder of the composition being iron and inevitable impurities resulting from the production process. The microstructure, given in surface proportions, comprises 13% to 25% residual austenite and 13% to 30% MA islands, the remainder consisting of bainite and possibly ferrite.

Abstract: Disclosed is a steel composition for hot stamping that comprises carbon (C) in an amount of about 0.22 to about 0.25 wt %, silicon (Si) in an amount of about 0.2 to about 0.3 wt %, manganese (Mn) in an amount of about 1.2 to about 1.4 wt %, titanium (Ti) in an amount of about 0.02 to about 0.05 wt %, chromium (Cr) in an amount of about 0.11 to about 0.2 wt %, boron (B) in an amount of about 0.005 to about 0.01 wt %, zinc (Zr) in an amount of about 0.005 to about 0.02 wt %, niobium (Nb) in an amount of about 0.01 to about 0.05 wt %, tungsten (W) in an amount of about 0.1 to about 0.5 wt %, iron (Fe) constituting the remaining balance of the steel composition, all the wt % based on the total amount of the steel composition.

Abstract: Method for producing elemental rhodium, comprising the steps of: (1) providing an aqueous suspension of diethylene triammonium hexahalogenorhodate adjusted with hydrohalic acid to a pH value of ?1 to +2; (2) adding a sufficient quantity of reducing agent for complete conversion of the diethylene triammonium hexahalogenorhodate to the suspension provided in step (1) and allowing the reaction to proceed until the formation of elemental rhodium is completed; and (3) separating the elemental rhodium formed in step (2) from the hydrohalic aqueous composition formed in step (2); whereby halogen is bromine and/or chlorine.

Abstract: An armor component produced from a 7xxx series aluminum alloy, wherein the aluminum alloy consists essentially of: 8.4 wt. %?Zn?10.5 wt. %; 1.3 wt. %?Mg?2 wt. %; 1.2 wt. %?Cu?2 wt. %; at least one dispersoid forming element with a total dispersoid forming element content higher than 0.05 wt. %; the remainder substantially aluminum, incidental elements and impurities.

Abstract: A method of manufacturing an iron-based alloy coating is provided, which includes (a) providing an iron-based alloy powder having a chemical formula of FeaCrbMocSidBeYf, wherein 48?a?50; 21?b?23; 18?c?20; 2?d?3; 2?e?4; and 0<f?2. The method also includes step (b) thermal spraying the iron-based alloy powder to form an amorphous iron-based alloy coating, and step (c) laser re-melting the amorphous iron-based alloy coating, wherein the iron-based alloy coating is densified and remains amorphous.

Abstract: The present invention relates to a method for preparing an aluminum-copper-iron quasicrystal and silicon carbide mixed reinforced aluminum matrix composite, where the aluminum-copper-iron quasicrystal and silicon carbide mixed reinforced aluminum matrix composite is prepared with an aluminum alloy serving as a matrix and with aluminum-copper-iron quasicrystal and silicon carbide serving as reinforcement agents via smelting in an intermediate-frequency induction melting furnace through the process of intermediate-frequency induction heating, vacuumizing, bottom blowing argon, and casting molding in view of low hardness and low tensile strength of aluminum matrix materials. The prepared aluminum-copper-iron quasicrystal and silicon carbide mixed reinforced aluminum matrix composite has a hardness of 80.3 HB which is improved by 50.64% and tensile strength of 285 Mpa which is improved by 60.42%, and corrosion resistance thereof is improved by 40%.

Abstract: An aluminum alloy for microporous hollow shape material use constituted by an aluminum alloy which has excellent corrosion resistance and extrudability which keeps down the content of Cu which poses a problem in intergranular corrosion resistance, which maintains the natural potential noble, which adds transition metals which do not obstruct the extrudability is provided, that is, an aluminum alloy which has a chemical composition which contains, by mass %, Fe: 0.05 to 0.20%, Si: 0.10% or less, Cu: 0.15 to 0.32%, Mn: 0.08 to 0.15%, Zr: 0.05% or less, Ti: 0.06 to 0.15%, Cr: 0.03% or less, and balance: Al and unavoidable impurities and has an amount of gas in the alloy billet of 0.25 cc/100 g or less, and a method of production of an aluminum alloy which, produces a DC cast billet.

Abstract: A method for coating a component is provided. The coating method includes providing a component having at least one aperture with an aperture geometry formed in a surface thereof, positioning a filler material within the at least one aperture, the filler material extending away from the surface of the component a distance greater than a reduced coating thickness and less than an applied coating thickness, applying at least one coating over the surface of the component and the filler material to form an applied coating having the applied coating thickness, removing a portion of the applied coating to provide the reduced coating thickness and expose the filler material, and removing the filler material to extend the at least one aperture having the aperture geometry through the applied coating. Another coating method and a template for use with the coating methods are also provided.

Abstract: The honeycomb structure includes a pillar-shaped honeycomb structure body having porous partition walls 1 defining a plurality of cells and a circumferential wall, and a pair of electrode members disposed on the side of a side surface of the honeycomb structure body. The pair of electrode members contain metal silicon and boron, at least a part of the electrode member is made of a composite material including, as a main component, silicon containing 100 to 10000 ppm of boron in silicon. In the composite material which is comprised the electrode member, a volume ratio of the silicon containing 100 to 10000 ppm of the boron in the composite material is 70 volume % or more. An electric resistivity of the electrode member made of the composite material is from 20 ??cm to 0.1 ?cm.

Abstract: A method for treating sheet metal is disclosed in which a material containing at least one alloying element is applied onto a first area of at least one surface of the metal sheet. A second area of the surface is kept free of the material. The metal sheet is subsequently heat treated in order to diffuse the alloying element into the first area of the metal sheet. The temperature of the first area is lower than the melting temperature of the metal sheet during the diffusion.

Abstract: A deposition device according to one embodiment includes a processing container. A mounting table is installed inside the processing container, and a metal target is installed above the mounting table. Further, a head is configured to inject an oxidizing gas toward the mounting table. This head is configured to move between a first region that is defined between the metal target and a mounting region where a target object is mounted on the mounting table and a second region spaced apart from a space defined between the metal target and the mounting region.

Abstract: A method for fabricating structures includes on a substrate includes providing a set of control parameters to an ion beam source and to a thermal source corresponding to a desired structure topology. The method also includes using directed irradiation synthesis to form nano structures and/or microstructures in a first surface area of the substrate by exposing the substrate surface to an ion beam from the ion beam source and to thermal particles from the thermal source. The ion beam having a first area of effect on the substrate surface, and the thermal particles having a second area of effect on the substrate surface, each of the first area of effect and the second area of effect including the first surface area.

Abstract: Some embodiments of the present disclosure provide a sputtering apparatus including a magnetron structure configured to erode a target according to a predetermined erosion rate profile symmetric to a central axis of the magnetron structure. The predetermined erosion rate profile includes a first peak rate in proximity to the central axis; and a second peak rate located at about from 0.7 to 0.75 of a radius of the target from the central axis.

Abstract: A window film is disclosed. The window film includes: a flexible transparent base material; a first metal target material film, disposed on the surface of the flexible transparent base material; a first high refractive index compound film, disposed on the surface of the first metal target material film; a first metal oxide film, disposed on the surface of the first high refractive index compound film; a first silver-containing metal film, disposed on the surface of the first metal oxide film; a second metal target material film, disposed on the surface of the first silver-containing metal film; and a second high refractive index compound film, disposed on the surface of the second metal target material film. The window film has better adherence, and is less likely to peel off. In addition, the window film also has better oxidation resistance, and is less likely to be oxidized. Furthermore, the window film also has a better optical effect and heat insulation effect.

Abstract: Disclosed is a carbon thin-film device and method for manufacturing the same. The method includes forming a functional group on a surface of a substrate and functionalizing the substrate, and depositing a carbon thin film through ALD on the substrate in which the functional group is formed.