Patents Issued in June 18, 2019
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Patent number: 10323204Abstract: This disclosure provides low viscosity, low volatility aryl ether compounds represented by formula (1): and represented by formula (2): wherein R1 is the same or different and is a linear or branched alkyl group having from 1 to 20 carbon atoms; R2 is the same or different and is hydrogen or an alkyl group having from 1 to 4 carbon atoms; and n is the same or different and is a value from 0 to 8. This disclosure also provides processes for producing the aryl ether compounds, lubricating oil basestocks and lubricating oils containing one or more of the aryl ether compounds, and a method for improving one or more of solubility and dispersancy of polar additives and/or sludge in a lubricating oil by using as the lubricating oil a formulated oil containing one or more of the aryl ether compounds.Type: GrantFiled: September 25, 2014Date of Patent: June 18, 2019Assignee: EXXONMOBIL RESEARCH AND ENGINEERING COMPANYInventors: Abhimanyu O. Patil, Satish Bodige
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Patent number: 10323205Abstract: A method for reducing timing chain stretch in an engine comprising a step of lubricating said timing chain with a lubricating oil composition that includes a major amount of a base oil; and a minor amount of an additive package including (a) at least one overbased calcium detergent, (b) at least one borated dispersant, (c) a metal dialkyl dithiophosphate, and (d) at least one molybdenum compound. The lubricating oil composition has a TBN value of at least 7.5 mg KOH/g of the lubricating oil composition, at least 80 ppm of molybdenum, based on the total weight of the lubricating oil composition, a weight ratio of total calcium in the lubricating oil composition to total molybdenum in the lubricating oil composition of less than 8.4; and a weight ratio of nitrogen from the dispersant in the lubricating composition to total boron in the lubricating oil composition of from 2.6 to 3.0.Type: GrantFiled: May 5, 2016Date of Patent: June 18, 2019Assignee: AFTON CHEMICAL CORPORATIONInventors: Kristin Fletcher, William Y. Lam
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Patent number: 10323206Abstract: The invention provides a lubricating grease composition comprising an oil of lubricating viscosity, a metallic soap thickener and a boron-containing compound, wherein the boron containing compound comprises a borate ester comprising at least one alkyl group having a branch at the ? position or higher. The invention further relates to a method of lubricating a mechanical device with the lubricant composition.Type: GrantFiled: January 26, 2016Date of Patent: June 18, 2019Assignee: The Lubrizol CorporationInventor: Gareth Fish
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Patent number: 10323207Abstract: A gear or engine oil or other type of lubricant, which effectively reduces churning losses in a dip lubrication system or any lubrication system where churning loss occur has a surface tension less than 28 mN/m and viscosity less than 400 mPa-sec at 25° C. (about 500 cSt at 25° C.). Formulations include Group I-IV base oil, in combination with an amount of silicone oil effective to decrease the surface tension of the oil, thereby reducing churning losses. When the base oil is prominently Group III, the coefficient of friction of the gear oil is also reduced.Type: GrantFiled: November 20, 2014Date of Patent: June 18, 2019Assignees: Imperial Innovations Limited, Valvoline Licensing and Intellectual Property LLCInventors: Anant S. Kolekar, Andrew V. Olver, Adam E. Sworski, Frances E. Lockwood, Gefei Wu, Xiurong Cheng
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Patent number: 10323208Abstract: The present invention provides a refrigerating machine oil containing an ester base oil obtained from pentaerythritol and mixed fatty acids of a fatty acid having 8 carbon atoms and a fatty acid having 9 carbon atoms, wherein a ratio of a weight average molecular weight Mw to a number average molecular weight Mn, Mw/Mn, of the ester base oil is 1.06 to 1.20, and used with a mildly flammable hydrofluorocarbon refrigerant.Type: GrantFiled: October 22, 2015Date of Patent: June 18, 2019Assignee: JXTG Nippon Oil & Energy CorporationInventors: Hitoshi Takahashi, Hiroko Shimpo, Souichirou Konno, Ken Sawada, Kentaro Yamaguchi
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Patent number: 10323209Abstract: The present invention relates to extracted lipid with high levels, for example 90% to 95% by weight, oleic acid. The present invention also provides genetically modified plants, particularly oilseeds such as safflower, which can used to produce the lipid. Furthermore, provided are methods for genotyping and selecting plants which can be used to produce the lipid.Type: GrantFiled: April 24, 2013Date of Patent: June 18, 2019Assignee: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATIONInventors: Craig Christopher Wood, Qing Liu, Xue-Rong Zhou, Allan Green, Surinder Pal Singh, Shijiang Cao
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Patent number: 10323210Abstract: The invention relates to a vetiveryl ester comprising at least 0.5 weight percent of secondary alcohol compounds with respect to the total weight of the vetiveryl ester and to a process for the preparation a vetiveryl ester, the process comprising: providing a vetiver oil, at least one enzyme preparation and at least one acylating compound; and allowing sufficient time for the enzyme preparation to esterify the alcohol compounds of the vetiver oil with the acylating compound. The invention also relates to a vetiveryl ester obtainable by the above process, a fragrance composition comprising an above-mentioned vetiveryl ester and the use of an above-mentioned vetiveryl ester for the preparation of a perfumed product.Type: GrantFiled: May 30, 2016Date of Patent: June 18, 2019Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE—CNRS, UNIVERSITÉ DE NICE-SOPHIA ANTIPOLISInventors: Sylvain Antoniotti, Jean-Jacques Filippi, Irene Notar Francesco, Jade Ramilijaona
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Patent number: 10323211Abstract: A hybrid mint plant characterized by an essential oil composition profile, methods of cultivating the hybrid mint plant, and methods of producing an essential oil composition with the essential oil composition profile using the hybrid mint plant are disclosed.Type: GrantFiled: May 15, 2017Date of Patent: June 18, 2019Assignee: Essex Laboratories, LLCInventor: Donald D. Roberts
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Patent number: 10323212Abstract: Polyols are produced by an alkoxylation process in which a vegetable oil containing hydroxyl functional groups is combined with a DMC catalyst to form a mixture, the DMC catalyst is then activated by adding ethylene oxide and/or propylene oxide to the vegetable oil/catalyst mixture, and ethylene oxide and propylene oxide are added to the mixture containing activated DMC catalyst in amounts such that the total of percentage of ethylene oxide in the polyol plus percentage of primary hydroxyl groups in the polyol produced is from 50 to 77% and the percentage of primary hydroxyl groups is at least 30% but less than 50%. These polyols are useful for the production of molded polyurethane foams, particularly, hot-cure molded polyurethane foams.Type: GrantFiled: April 15, 2011Date of Patent: June 18, 2019Assignee: Covestro LLCInventors: Stanley L. Hager, Micah N. Moore, Jack R. Reese, Brian L. Neal
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Patent number: 10323213Abstract: The present application relates to a detergent with an improved cleaning performance, in particular on greasy stains, said detergent comprising a surfactant mixture containing amine oxide, alkylbenzene sulfonate, alkyl ether sulfate, and fatty alcohol ethoxylate. The invention also relates to the use of said detergent and to a method for improving the cleaning performance of a detergent.Type: GrantFiled: January 26, 2016Date of Patent: June 18, 2019Assignee: Henkel AG & Co. KGaAInventors: Inga Kerstin Vockenroth, Eva-Maria Wikker
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Patent number: 10323214Abstract: The need for a liquid hard surface cleaning composition which provides antimicrobial efficacy and good cleaning is met by formulating the composition using an antimicrobial active in combination with an amine oxide surfactant and additional non-ionic surfactant at the desired ratio.Type: GrantFiled: December 13, 2016Date of Patent: June 18, 2019Assignee: The Procter & Gamble CompanyInventors: Laura Cermenati, Raphael Angeline Alfons Ceulemans, Ann Vandevenne
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Patent number: 10323215Abstract: The present invention relates to a process for preparing a polymer composition, in which an ?,?-ethylenically unsaturated carboxylic acid is subjected to a free-radical polymerization in the presence of at least one polyether component, to the polymer composition obtainable by this process and to the use thereof.Type: GrantFiled: July 2, 2014Date of Patent: June 18, 2019Assignee: BASF SEInventors: Yannick Fuchs, Helmut Witteler, Heike Weber, Alejandra Garcia Marcos, Jürgen Detering
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Patent number: 10323216Abstract: A cleaning agent for hard surfaces and methods for cleaning are provided herein. In one embodiment, the cleaning agent includes at least one first amylase, wherein the first amylase is an ?-amylase from Bacillus sp. No. 707 or a functional fragment or a variant thereof. The cleaning agent further includes at least one second amylase, wherein the second amylase is an AA560 ?-amylase from Bacillus sp. or a functional fragment or a variant thereof. In another embodiment, the method includes dispensing the cleaning agent into the interior of an automatic dishwasher while a dishwashing program is being executed, before the main washing cycle begins, or in the course of the main washing cycle.Type: GrantFiled: June 18, 2015Date of Patent: June 18, 2019Assignee: Henkel AG & Co. KGaAInventors: Inga Kerstin Vockenroth, Thomas Weber, Silke Menke, Thomas Eiting, Nina Mussmann, Thorsten Bastigkeit, Noelle Wrubbel
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Patent number: 10323217Abstract: The disclosure concerns a detergent composition comprising one or more anionic surfactants; an enzyme selected from the group consisting of: a protease, a lipase, a cutinase, an amylase, a carbohydrase, a cellulase, a pectinase, a mannanase, an arabinase, a galactanase, a xylanase, and an oxidase; and a deoxyribonuclease (DNase), as well as methods for washing a textile.Type: GrantFiled: December 9, 2013Date of Patent: June 18, 2019Assignee: Novozymes A/SInventors: Klaus Gori, Lilian Eva Tang Baltsen, Marie Allesen-Holm
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Patent number: 10323218Abstract: The present invention relates to novel sulfoperoxycarboxylic acid compounds, and methods for making and using them. The sulfoperoxycarboxylic compounds of the invention are storage stable, water soluble and have low to no odor. Further, the compounds of the present invention can be formed from non-petroleum based renewable materials. The compounds of the present invention can be used as antimicrobials, and bleaching agents. The compounds of the present invention are also suitable for use as coupling agents.Type: GrantFiled: June 4, 2018Date of Patent: June 18, 2019Assignee: Ecolab USA Inc.Inventors: Junzhong Li, Richard K. Staub, David D. McSherry, Keith G. Lascotte, Steven J. Lange, Frank Everts
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Patent number: 10323219Abstract: A process for reblending a first liquid detergent composition into a second liquid detergent composition.Type: GrantFiled: July 18, 2017Date of Patent: June 18, 2019Assignee: The Procter & Gamble CompanyInventors: Neil Joseph Lant, Nazarmohammad Gulamhussain Momin, Antonio Quintieri, Steven George Patterson
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Patent number: 10323220Abstract: The present invention relates to water-soluble unit dose articles comprising an amphoteric surfactant and a cyclic diamine, and methods of use thereof.Type: GrantFiled: July 31, 2017Date of Patent: June 18, 2019Assignee: The Procter & Gamble CompanyInventors: Nigel Patrick Somerville Roberts, Alan Thomas Brooker, Colin Ure
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Patent number: 10323221Abstract: A device for controlling apical flow to a cell culture includes an apical insert that defines at least one inlet channel extending from an inlet port to an apical feed port and at least one outlet channel extending from an apical effluent port to an outlet port. The apical insert includes a projecting portion configured to extend into a cell culture insert to a depth that is less than a depth of the cell culture insert, and a contact surface configured to maintain a spatial relationship between the projecting portion and the cell culture insert.Type: GrantFiled: January 5, 2017Date of Patent: June 18, 2019Assignees: Northeastern University, Massachusetts Institute of TechnologyInventors: Transon V. Nguyen, Collin D. J. Edington, Emily C. Suter, Rebecca Lyn Carrier, David L. Trumper, Linda G. Griffith
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Patent number: 10323222Abstract: The invention relates to a device (1) for automated inoculation of a culture medium, the device being of the type comprising: a clamp (2) suitable for gripping the inoculation tool; a clamp-holder (3); a support base (4) carrying the assembly formed by the clamp (2) and the clamp-holder (3); and a support structure (5) for supporting the support base (4) and for enabling the support base (4) to be moved along three axes.Type: GrantFiled: May 20, 2014Date of Patent: June 18, 2019Assignee: INTELLIGENCE ARTIFICIELLE APPLICATIONSInventors: Christian Curel, Michel Roch, Jean-Louis Cariou
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Patent number: 10323223Abstract: A vessel having a mixer that ensures a homogeneous cell distribution in dispensed quantities. The vessel has a mixer therein for stirring contents of the vessel and an orifice in a lower wall to which a cell dispenser is attached. The cell dispenser dispenses quantities of suspended cells having a homogeneous cell distribution.Type: GrantFiled: January 23, 2017Date of Patent: June 18, 2019Assignee: PBS Biotech, Inc.Inventors: Chanyong Brian Lee, Yasunori Hashimura, Oscar Garza, Gary Evans
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Patent number: 10323224Abstract: A vibration generator causes a carrier to oscillate, rather than the housing of the vibration generator. The vibration generator is placed within the housing, which itself is not induced to oscillate, thus reducing or eliminating undesired noises due to excessive oscillations of the housing. The vibration generator transfers vibrations to the carrier for Petri dishes using at least one connecting element that is arranged between the carrier and the vibration generator. The connecting element protrudes through the wall of the housing and is sealed by a sealing element with respect to the wall.Type: GrantFiled: February 6, 2015Date of Patent: June 18, 2019Inventor: Alireza Rahimi
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Patent number: 10323225Abstract: A fermentation system includes a fermentation tank, a plurality of sensors operable to measure a plurality of fermentation system parameters, a plurality of mechanisms operable to control the fermentation system parameters, and a controller in electronic communication with the sensors and with the mechanisms. The controller includes a memory, a receiver circuit, a transmitting circuit, and a control circuit. The memory stores a plurality of recipes each defining user operating instructions and parameter settings. The receiver circuit is configured to receive a batch identification code associated in the memory with one of the recipes. The transmitting circuit is configured to communicate the user operating instructions defined by the associated one of the recipes. The control circuit is configured to operate the mechanisms to adjust the fermentation system parameters in accordance with the parameter settings defined by the associated one of the recipes.Type: GrantFiled: October 20, 2016Date of Patent: June 18, 2019Assignee: Locus SolutionsInventors: Sean Farmer, Sharmistha Mazumder, Xiao-Zhou Zhang, Maja Milovanovic
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Patent number: 10323226Abstract: A bacteria starter material for use in biomass generators without causing jamming of the feeder mechanism, particularly under high temperature and/or high humidity conditions, the starter material comprising one or more species of waste-decomposing bacteria, sucrose, and a soy based protein. Preferably, DI-PAC® sugar (sucrose and maltodextrin) and powdered KIKKOMAN® Soy Sauce are used as the sugar and protein ingredients. The starter material may also comprise a dried bran culture comprising Pseudomonas. The starter material ingredients are blended together and formed into a tablet or pellet having a particular shape, size, and hardness characteristics suitable for use in the feeder mechanism of a biomass generator.Type: GrantFiled: March 30, 2017Date of Patent: June 18, 2019Assignee: NCH CorporationInventors: Judith G. Pruitt, Robert C. Pearce, III, Daniel Aberle, Charles J. Greenwald, Gabriel F. K. Everett
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Patent number: 10323227Abstract: Disclosed herein is a composition containing turmeric starch for use as a prebiotic plant fiber. Also disclosed is a method to increase the viable counts of Bacillus coagulans MTCC 5856 by co-culturing with turmeric starch and the production of short chain fatty acids (SCFA) by Bacillus coagulans MTCC 5856 using turmeric starch.Type: GrantFiled: July 27, 2017Date of Patent: June 18, 2019Assignee: SAMI LABS LIMITEDInventors: Muhammed Majeed, Kalyanam Nagabhushanam, Sivakumar Arumugam, Furqan Ali
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Patent number: 10323228Abstract: Disclosed are methods of differentiating stem cells in order to obtain hepatocyte-like cells, the method comprising the steps of a) subjecting definitive endoderm to at least one epigenetic modulator to obtain hepatoblasts and b) subjecting the hepatoblasts to at least one stem cell differentiation pathway inhibitor to obtain hepatocyte-like cells; wherein steps a) and b) do not comprise the use of a growth factor. In one preferred embodiment, the epigenetic modulator may be sodium butyrate and/or DMSO and the stem cell differentiation pathway inhibitor may be SB431542 and/or DMSO. Also disclosed are hepatocyte-like cells obtained from the method and uses of these cells such as drug screening.Type: GrantFiled: September 17, 2015Date of Patent: June 18, 2019Assignee: Agency for Science, Technology and ResearchInventors: Hanry Yu, Derek Phan, Yi-chin Toh, Farah Tasnim
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Patent number: 10323229Abstract: The present invention provides a new therapeutic method for modeling neuronal responses. Human induced pluripotent stem cells (hiPSCs) emerge recently as alternative sources of primary brain cells to establish Alzheimer's disease (AD) models in vitro. While previous investigations demonstrate the potential of hiPSCs in modeling AD, the 2-D cultures used in these studies cannot fully recapitulate AD-associated neuropathology. 3-D cortical spheroids (forebrain-like structure) were derived from human induced pluripotent stem cells in a bioreactor culture, which can be used to recapitulate AD-associated neuropathology, to screen the therapeutic drugs and to predict neurotoxicity.Type: GrantFiled: November 14, 2016Date of Patent: June 18, 2019Assignee: The Florida State University Research Foundation, Inc.Inventor: Yan Li
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Patent number: 10323230Abstract: The present invention includes a method for preparing a three-dimensionally cultured skin model comprising dermis and epidermis, which comprises: a step of preparing the dermis using a composition comprising murine fibroblasts; and a combination of native collagen and atelocollagen; and a step of forming the epidermis using keratinocytes. Also, the present invention relates to a three-dimensionally cultured skin model which comprises: a dermis prepared by a composition comprising murine fibroblasts, native collagen, or a combination of native collagen and atelocollagen; and epidermis formed from keratinocytes.Type: GrantFiled: July 9, 2015Date of Patent: June 18, 2019Inventor: Saewha Jeon
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Patent number: 10323231Abstract: Provided herein are attenuated influenza viruses and methods of making attenuated influenza viruses.Type: GrantFiled: April 22, 2016Date of Patent: June 18, 2019Assignee: UNIVERSITY OF ROCHESTERInventors: Andrew Cox, Stephen Dewhurst, John Treanor
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Patent number: 10323232Abstract: This disclosure relates to the engineering of phototrophic microorganisms for conversion of alkanes into higher-value products. Recombinant phototrophic organisms such as cyanobacteria can be engineered, optionally in a modular format, to express enzymes involved in converting methane to methanol, methanol to formaldehyde, formaldehyde to central metabolic pathway intermediates, and such intermediates to n-butanol.Type: GrantFiled: October 14, 2014Date of Patent: June 18, 2019Assignee: MOgene LCInventors: Abhay Singh, Himadri Pakrasi, Ganesh M. Kishore
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Patent number: 10323233Abstract: The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.Type: GrantFiled: March 6, 2018Date of Patent: June 18, 2019Assignee: Codexis, Inc.Inventors: Ish K. Dhawan, Gregory Miller, Xiyun Zhang
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Patent number: 10323234Abstract: The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.Type: GrantFiled: June 26, 2018Date of Patent: June 18, 2019Assignee: Codexis, inc.Inventors: Ish K. Dhawan, Gregory Miller, Xiyun Zhang
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Patent number: 10323235Abstract: Disclosed are compositions comprising an engineered intein designed such that the self-cleaving activity of the intein can be modulated by a zinc-binding motif as well as methods and systems for making and using the compositions.Type: GrantFiled: September 18, 2017Date of Patent: June 18, 2019Assignee: Ohio State Innovation FoundationInventors: Buyong Ma, David F. Nellis, Jianwei S. Zhu, David W. Wood
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Patent number: 10323236Abstract: Engineered nucleases (e.g., zinc finger nucleases (ZFNs), transcriptional activator-like effector nucleases (TALENs), and others) are promising tools for genome manipulation and determining off-target cleavage sites of these enzymes is of great interest. We developed an in vitro selection method that interrogates 1011 DNA sequences for their ability to be cleaved by active, dimeric nucleases, e.g., ZFNs and TALENs. The method revealed hundreds of thousands of DNA sequences, some present in the human genome, that can be cleaved in vitro by two ZFNs, CCR5-224 and VF2468, which target the endogenous human CCR5 and VEGF-A genes, respectively. Our findings establish an energy compensation model of ZFN specificity in which excess binding energy contributes to off-target ZFN cleavage and suggest strategies for the improvement of future nuclease design. It was also observed that TALENs can achieve cleavage specificity similar to or higher than that observed in ZFNs.Type: GrantFiled: July 22, 2012Date of Patent: June 18, 2019Assignee: President and Fellows of Harvard CollegeInventors: David R. Liu, John Paul Guilinger, Vikram Pattanayak
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Patent number: 10323237Abstract: A lysozyme having improved enzymatic activity is disclosed. The amino acid sequence of the lysozyme is a modified amino acid sequence of SEQ ID NO: 2 or a modified amino acid sequence with at least 80% sequence identity of SEQ ID NO: 2, wherein the modification is a substitution of histidine at position 166 or a corresponding position with lysine.Type: GrantFiled: August 21, 2018Date of Patent: June 18, 2019Assignee: CHENGDU BEACON BIO-TECHNOLOGY CO., LTD.Inventors: Chun-Chi Chen, Tao Wang, Jian-Wen Huang, Rey-Ting Guo
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Patent number: 10323238Abstract: The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.Type: GrantFiled: June 28, 2017Date of Patent: June 18, 2019Assignee: HYGLOS INVEST GMBHInventors: Bernd Buchberger, Holger Grallert, Sonja Molinaro
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Patent number: 10323239Abstract: Cell-targeted serine protease constructs are provided. Such constructs can be used in methods for targeted cell killing such as for treatment cell of proliferative diseases (e.g., cancer). In some aspects, recombinant serine proteases, such as Granzyme B polypeptides, are provided that exhibit improved stability and cell toxicity. Methods and compositions for treating lapatinib or trastuzumab-resistant cancers are also provided.Type: GrantFiled: March 9, 2018Date of Patent: June 18, 2019Assignee: Research Development FoundationInventors: Michael G. Rosenblum, Khalid Amanali Mohamedali, Lawrence H. Cheung
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Patent number: 10323240Abstract: One aspect of the present invention relates to mutants of chondroitinase ABCI. Such chondroitinase ABCI mutants exhibit altered chondroitin lyase activity or increased resistance to inactivation from stressors including exposure to UV light or heat. Methods of using chondroitinase ABCI mutant enzymes are also provided.Type: GrantFiled: October 20, 2017Date of Patent: June 18, 2019Assignee: Acorda Therapeutics, Inc.Inventors: Anthony O. Caggiano, Jennifer Iaci, Andrea Vecchione, Elizabeth Hunter
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Patent number: 10323241Abstract: A method of recovering short-chain nucleic acids having a length of 1000 bases or less from a whole blood sample, which includes bringing a first mixture including a whole blood sample into contact with a first nucleic acid adsorbent to adsorb nucleic acids derived from the whole blood sample, removing the first nucleic acid adsorbent from being in contact with the first mixture to recover liquid components of the first mixture, bringing a second mixture including the recovered liquid components into contact with a second nucleic acid adsorbent to adsorb short-chain nucleic acids having a length of 1000 bases or less from the whole blood sample, separating the second nucleic acid adsorbent from being in contact with the second mixture, and eluting the short-chain nucleic acids absorbed in the second nucleic acid adsorbent and recovering the short-chain nucleic acids from the second nucleic acid adsorbent.Type: GrantFiled: October 13, 2016Date of Patent: June 18, 2019Assignee: TOPPAN PRINTING CO., LTD.Inventor: Kei Tsukamoto
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Patent number: 10323242Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.Type: GrantFiled: February 7, 2019Date of Patent: June 18, 2019Assignee: Inscripta, Inc.Inventors: Don Masquelier, Phillip Belgrader, Jorge Bernate, Ryan Gill, Kevin Ness
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Patent number: 10323243Abstract: Embodiments of the disclosure concern methods and compositions related to generation and/or use of proofreading reverse transcriptases, including those that are thermophilic or hyperthermophilic. The disclosure encompasses specific recombinant polymerases and their use. In some embodiments, the polymerases are utilized for RNA sequencing in the absence of generation of a cDNA intermediate.Type: GrantFiled: January 19, 2017Date of Patent: June 18, 2019Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Andrew Ellington, Jared Ellefson, Jimmy Gollihar
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Patent number: 10323244Abstract: Provided are an LncRNA and oncolytic adenovirus, and application thereof. The oncolytic adenovirus is used as a carrier to express the LncRNA, so as to express the LncRNA in a cancer cell; competitively binding a target gene of OncomiRs, and consuming the OncomiRs, thus protecting a cancer suppressor gene from interference and suppression of the OncomiRs, and achieving target intervention therapy of the cancer cell.Type: GrantFiled: October 14, 2015Date of Patent: June 18, 2019Assignee: SECOND MILITARY MEDICAL UNIVERSITY OF THE PEOPLE'S LIBERATION ARMYInventors: Changqing Su, Yinghan Su, Xiaoya Li, Weidan Ji, Kouyong Liu
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Patent number: 10323245Abstract: The invention is to methods of gene silencing in arthropods using dsRNA. The method is include contacting the arthropod with, and/or directly feeding the arthropod, the dsRNA to the arthropods to deliver the dsRNA to arthropod tissues. It is envisaged that the methods of the invention will have use in determining the biological function of genes in arthropods. Methods of pest control of arthropods, and of protecting arthropods against parasites and predators are provided. Transgenic arthropods expressing dsRNA molecules are also provided by the present invention.Type: GrantFiled: May 22, 2017Date of Patent: June 18, 2019Assignee: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATIONInventors: Steven Whyard, Fiona Helen Cameron, Minoo Moghaddam, Trevor J. Lockett
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Patent number: 10323246Abstract: The present invention is related to a ribonucleic acid comprising a double stranded structure whereby the double-stranded structure comprises a first strand and a second strand, whereby the first strand comprises a first stretch of contiguous nucleotides and whereby said first stretch is at least partially complementary to a target nucleic acid, and the second strand comprises a second stretch of contiguous nucleotides whereby said second stretch is at least partially identical to a target nucleic acid, and whereby the double stranded structure is blunt ended.Type: GrantFiled: August 15, 2017Date of Patent: June 18, 2019Assignee: Silence Therapeutics GMBHInventors: Klaus Giese, Jörg Kaufmann, Anke Klippel-Giese
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Patent number: 10323247Abstract: The invention discloses a method for improving the extracellular expression level of a foreign protein by means of phospholipase fusion expression. Four proteins, PLA2, MBP, CBD and SUMO, are used as a fusion tag to construct a fusion gene. Compared with an original protein MOH without any fusion tag, the extracellular expression level and enzymatic activity of all the four fusion proteins are increased to some degree. Among them, the fusion protein using PLA2 as the fusion tag has the highest expression level, which is 7.4 times higher than that of the original protein. Compared with other fusion tags, PLA2 has a low molecular weight and the fusion protein having PLA2 as the fusion tag has the highest expression level (up to 12 g·L?1 in a 7 L fermentation tank for high-density fermentation). It is shown that the secretory expression of a foreign protein can be effectively increased by using PLA2 as a fusion tag.Type: GrantFiled: June 1, 2018Date of Patent: June 18, 2019Assignee: Jiangnan UniversityInventors: Xiaowei Yu, Yan Xu
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Patent number: 10323248Abstract: Control Devices are disclosed including RNA destabilizing elements (RDE), RNA control devices, and destabilizing elements (DE) combined with Chimeric Antigen Receptors (CARs) or other transgenes in eukaryotic cells. Multicistronic vectors are also disclosed for use in engineering host eukaryotic cells with the CARs and transgenes under the control of the control devices. These control devices can be used to optimize expression of CARs in the eukaryotic cells so that, for example, effector function is optimized. CARs and transgene payloads can also be engineered into eukaryotic cells so that the transgene payload is expressed and delivered after stimulation of the CAR on the eukaryotic cell.Type: GrantFiled: August 31, 2017Date of Patent: June 18, 2019Assignee: Chimera Bioengineering, Inc.Inventors: Benjamin Wang, Gusti Zeiner
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Patent number: 10323249Abstract: Control Devices are disclosed including RNA destabilizing elements (RDE), RNA control devices, and destabilizing elements (DE) combined with Chimeric Antigen Receptors (CARs) or other transgenes in eukaryotic cells. Multicistronic vectors are also disclosed for use in engineering host eukaryotic cells with the CARs and transgenes under the control of the control devices. These control devices can be used to optimize expression of CARs in the eukaryotic cells so that, for example, effector function is optimized. CARs and transgene payloads can also be engineered into eukaryotic cells so that the transgene payload is expressed and delivered after stimulation of the CAR on the eukaryotic cell.Type: GrantFiled: October 3, 2018Date of Patent: June 18, 2019Assignee: Chimera Bioengineering, Inc.Inventors: Benjamin Wang, Gusti Zeiner
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Patent number: 10323250Abstract: The present invention aims to provide a promoter showing high expression activity in microorganisms belonging to the genus Mortierella. The present invention provides a polynucleotide which contains any one nucleotide sequence selected from the group consisting of SEQ ID NO: 1 to 28, or a variant thereof.Type: GrantFiled: September 19, 2018Date of Patent: June 18, 2019Assignee: SUNTORY HOLDINGS LIMITEDInventors: Misa Ochiai, Jun Ogawa, Eiji Sakuradani, Akinori Ando
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Patent number: 10323251Abstract: A method for producing a plant cell comprising a mutation introduced in a target DNA comprises: a step of introducing into plant cells a DNA construct comprising a DNA homologous to a target DNA, wherein a desired mutation is introduced and a piggyBac transposon containing a marker gene is inserted in the homologous DNA; a step of selecting a plant cell, in which the mutation and the piggyBac transposon are introduced in the target DNA via homologous recombination, based on an expression of the marker gene; and a step of removing the piggyBac transposon from the target DNA by constitutively expressing a piggyBac transposase in the cell selected in the above step.Type: GrantFiled: February 19, 2015Date of Patent: June 18, 2019Assignee: NATIONAL RESEARCH AND DEVELOPMENT AGENCY NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATIONInventors: Ayako Yokoi, Seiichi Toki
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Patent number: 10323252Abstract: Provided herein are plants and methods to increase plant biomass, for example, a transgenic plant, plant cell, plant part or seed, wherein the transgenic plant, plant cell, plant part or seed comprises a heterologous promoter which increases expression of SWAM1, other genes related to SWAM1, or GNRF, as compared to a non-transgenic plant.Type: GrantFiled: June 30, 2016Date of Patent: June 18, 2019Assignee: University of MassachusettsInventors: Samuel P. Hazen, Pubudu Handakumbura
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Patent number: 10323253Abstract: This disclosure provides recombinant DNA constructs and transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.Type: GrantFiled: December 2, 2015Date of Patent: June 18, 2019Assignee: Monsanto Technology LLCInventors: Mark S. Abad, Erin Bell, Paul S. Chomet, Todd DeZwaan, Stephen Duff, Barry S. Goldman, Hongwu Jia, Lloyd Jones, Balasulojini Karunanandaa, Saritha V. Kuriakose, Linda L. Lutfiyya, Savitha Madappa, Zoe P. McKiness, Donald E. Nelson, Sasha Preuss, Monnanda S. Rajani, Dhanalakshmi Ramachandra, Aniruddha Raychaudhuri, Daniel P. Schachtman, Steven H. Schwartz, Char Shobha, Matthew M. Tanzer, Tyamagondlu V. Venkatesh, Huai Wang, Xiaoyun Wu