Abstract: The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

Abstract: Methods and apparatus providing for the isolation of an unknown mutation from a sample comprising wild type nucleic acids and mutated nucleic acids through the application of time-varying driving fields and periodically varying mobility-altering fields to the sample within in an affinity matrix.

Abstract: A method is disclosed for analyzing single nucleotide polymorphisms (SNPs) of mitochondrial DNA (mtDNA) using a peptide nucleic acid (PNA) probe and a melting curve analysis, and more particularly, a method is disclosed for analyzing SNPs of mtDNA by the melting curve analysis of a PNA probe and mtDNA, in which the PNA probe is coupled with a reporter and a quencher. By using PNA probes labeled with a reporter and a quencher in the method for analyzing SNPs of mtDNA, the time and cost required for individual identification can be drastically decreased, initial analysis can be carried out in the event of a large number of inspections or mass casualties, and human identification information can be coded, and thus the method is very easy to manage and supervise.

Abstract: Provided herein are dynamic flux nucleic acid sequence amplification methods. The dynamic flux nucleic acid sequence amplification methods described herein are capable of amplifying nucleic acid sequences within a narrow temperature range. In some aspects, the disclosure provides for real-time dynamic flux nucleic acid sequence amplification methods.

Abstract: A system for performing quality control for nucleic acid sample sequencing is disclosed. The system comprises a set of solid supports, each solid support having attached thereto a plurality of nucleic acid sequences, wherein the set comprises plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived, and the nucleic acids sequences are designed such that the nucleic acid sequences produce a predefined pattern of detectable signals during a sequencing run. A method of preparing a quality control for performing nucleic acid sample sequencing, a method of validating a nucleic acid sequencing instrument during a nucleic acid sequencing experiment, and a method of processing nucleic acid sequencing data during a nucleic acid sequencing experiment are also disclosed.

Abstract: A system for performing quality control for nucleic acid sample sequencing is disclosed. The system comprises a set of solid supports, each solid support having attached thereto a plurality of nucleic acid sequences, wherein the set comprises plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived, and the nucleic acids sequences are designed such that the nucleic acid sequences produce a predefined pattern of detectable signals during a sequencing run. A method of preparing a quality control for performing nucleic acid sample sequencing, a method of validating a nucleic acid sequencing instrument during a nucleic acid sequencing experiment, and a method of processing nucleic acid sequencing data during a nucleic acid sequencing experiment are also disclosed.

Abstract: The present method involves sequencing by synthesis in which a template strand having an attached primer is immobilized in a small volume reaction mixture. In one embodiment, the reaction mixture is in contact with a sensitive heat sensor, which detects the heat of reaction from incorporation of a complementary base (dNTP) in the presence of appropriate reagents (DNA polymerase, and polymerase reaction buffer). Alternatively, or in addition, a change in pH resulting from the incorporation of nucleotides in the DNA polymerase reaction is measured. A device is provided having delivery channels for appropriate reagents, including dNTPs, which may be delivered sequentially or in a mixture. Preferably, the dNTPs are added in a predetermined sequence, and the dNTP is incorporated or not depending on the template sequence.

Abstract: The invention relates to a method for method of characterizing, such as sequencing, a target double stranded polynucleotide. The polynucleotide is coupled to a membrane using at least two adaptors with different strengths of coupling to the membrane.

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

Abstract: Provided herein is a method for analyzing polynucleotides such as genomic DNA. In certain embodiments, the method comprises: (a) treating chromatin isolated from a population of cells with an insertional enzyme complex to produce tagged fragments of genomic DNA; (b) sequencing a portion of the tagged fragments to produce a plurality of sequence reads; and (c) making an epigenetic map of a region of the genome of the cells by mapping information obtained from the sequence reads to the region. A kit for performing the method is also provided.

Abstract: Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences.

Abstract: The purpose of the present invention is to develop a method for screening drugs having the effect of increasing skin barrier function in in vitro studies and to evaluate barrier function in the skin. Candidate drugs can be screened by using the activity and/or expression level of serine racemase as an indicator.

Abstract: In one embodiment, the present invention relates to a method for optimizing cell-type specific protein expression. In another embodiment, the present invention relates to a method for creating a tRNA profile of a cell.

Abstract: Provided are methods for amplifying a gene or RNA or sets thereof of interest using a tandem PCR process. The primers in the first PCR or set of PCR reactions are locus-specific. The primers in the second PCR or set of PCR reactions are specific for a sub-sequence of the locus-specific primers and completely consumed during the second PCR amplification. For RNA amplification, the first PCR is reverse transcription and the resulting cDNA(s) provide a template for cRNA synthesis, endpoint PCR or real time PCR. Also provided is a tandem PCR method which accepts raw, completely unpurified mouthwash, cheek swabs and ORAGENE-stabilized saliva as the sample input, the resulting amplicons serving as the substrate for complex, microarray-based genetic testing. Also provided is a method of allelotyping a gene or set thereof by amplifying the gene(s) using tandem PCR on DNA or RNA comprising the sample.

Abstract: Collections of polynucleotides useful for estimating breeding value or detecting likelihood of twinning are disclosed. The polynucleotides are used to detect genomic sequences quantitatively associated with the twinning trait. Also disclosed are methods and kits for using the collections to estimate breeding value or predict likelihood of twinning.

Abstract: Focal and segmental glomerulosclerosis (FSGS) is a kidney disorder of unknown etiology and up to 20% of patients on dialysis have this diagnosis. A large family with hereditary FSGS carries a missense mutation in the TRPC6 gene on chromosome 11q, encoding the ion channel protein Transient Receptor Potential Cation Channel 6. The missense mutation is a P112Q substitution, which occurs in a highly conserved region of the protein, enhances TRPC6-mediated calcium signals in response to agonists such as angiotensin II, and alters the intracellular distribution of TRPC6 protein. Previous work has emphasized the importance of cytoskeletal and structural proteins in proteinuric kidney diseases. Our findings suggest a novel mechanism for glomerular disease pathogenesis.

Abstract: The present invention concerns methods, in particular in vitro methods, for diagnosing liver fibrosis in a subject suffering from obesity, or for selecting a subject suffering from obesity for liver biopsy or for treatment. The present invention also pertains to methods for screening a probiotic, a prebiotic, a chemical compound or a biological compound suitable for preventing and/or treating liver fibrosis.

Abstract: The present invention relates, in part, to methods and kits for treating cardiovascular disease.

Abstract: The present invention provides a marker which can be used as an indicator for efficacy prediction of an mTOR related anticancer agent or prognostic prediction, and a novel anticancer agent. The present invention provides a method for efficacy evaluation of a cancer drug, and, specifically, a prediction method for the efficacy of an mTOR-related cancer drug by detecting NRF2 abnormality. In addition, the present invention provides a prognostic prediction method for cancer, and, specifically, a prediction method for the prognosis of cancer by detecting NRF2 abnormality. Furthermore, the present invention provides a novel anticancer agent that targets NRF2.

Abstract: Described herein are methods of measuring, in a genomic sample from an individual, the relative frequency of a target sequence (that is, its copy number) with respect to a control sequence of known copy number at a different genomic locus in the same genome, wherein the target and control sequences differ by at least one single nucleotide variation (SNV). These methods involve both the target sequence and the control sequence in a single reaction/container, using a pair of primers that prime amplification of both the target sequence and the control sequence, or a single downstream primer and two upstream primers (that differs only at the position of the SNV between the target and control sequences), and measuring the abundance of each of the target sequence and the control sequence using SNV-specific labeled probes or primers, or a melting curve analysis that distinguishes between the amplified control and target sequences. The methods are exemplified with various different amplifications process.

Abstract: Methods of identifying a single nucleotide polymorphism associated with a plant trait and methods of identifying a plant having an improved trait. The plant trait is correlated with at least one single nucleotide polymorphism in a microRNA region of a plant genome. Isolated nucleic acids, transgenic plants, and methods of producing the same are also disclosed.

Abstract: A method of gaseous substrate fermentation comprising: adding gaseous substrate comprising carbon monoxide (CO) and hydrogen (H2) into an aqueous medium in a bioreactor; said method comprising measuring conversion of CO; measuring conversion of H2; increasing flow of gaseous substrate by a preselected flow factor; wherein agitation comprises greater than or equal to target agitation rate.

Abstract: Reactor assemblies and methods for hydrolytic biomass conversion are disclosed herein. The reactor assemblies employ solely hydrostatic pressure, applied by the liquid biomass slurry, as means of providing pressure necessary to maintain high temperature conditions suitable for hydrolytic biomass conversion resulting in the formation of soluble pentose compounds. In embodiments, the methods employed in conjunction with the reactor assemblies yield soluble C5 carbohydrates in the forms of oligosaccharides and monosaccharides from one or more biomass slurries.

Abstract: A method for reducing metal oxide containing charge materials (1): reducing the metal oxide containing charge materials (1) in at least two fluidized bed units (RA,RE) by means of a reduction gas (2), wherein at least some of the resulting off-gas (3) is recycled and wherein the metal oxide containing charge materials (1) are conveyed into the fluidized bed unit RE by a propellant gas. Also, apparatus for carrying out the method according to the invention is disclosed.

Abstract: A method of producing steel by charging a furnace with scrap metal and agglomerated oxy-carbon material into a workspace of a furnace, to reduce specific electricity consumption when melting. Increasing the iron output quantity by inputting electric energy, fuel, a carburizer, a flux and gaseous oxygen, using electric arc melting with decarburization of a metal bath, and releasing metal and slag from the furnace. Prior to melting, a portion of the material is loaded with a first portion of the metal charge into the central zone of the furnace, and the remaining material into the melted charge during melting 0.5-10 kg/min per 1 megavolt-ampere of electric arc transformer power. The oxy-carbon material size is between 5 and 80 millimeters.

Abstract: Described are solid agglomerates of fine metal particles and methods for manufacturing same. A liquid oily lubricant is used in the manufacture of the solid agglomerates. The manufacturing comprises blending fine metal particles with the liquid oily lubricant and compacting the oily metallic mixture obtained to desired solid form. Advantageously, the solid agglomerates possess a desirable density, a suitable resistance to crumbling and dusting during handling, and they can resist to high temperature and to humidity. Solid agglomerated metal products, according to the invention, may be useful for different purposes such as quality charge material for steel plants, blast furnaces and foundries.

Abstract: The present invention relates to a maraging steel containing, in terms of mass %, 0.20?C?0.35, 9.0?Co?20.0, 1.0?(Mo+W/2)?2.0, 1.0?Cr?4.0, and a certain amount of Ni, with the balance being Fe and inevitable impurities, in which in a case where the contents of V and Nb satisfy V+Nb?0.020 mass %, the amount of Ni is 6.0?Ni?9.4, and in which in a case where the contents of V and Nb satisfy 0.020 mass %<V+Nb?0.60 mass %, the amount of Ni is 6.0?Ni?16.0.

Abstract: A process for the production of grain non-oriented electric Fe—Si steel strips, with excellent electric and/or magnetic characteristics to be used preferably for construction of electrical machines is disclosed.

Abstract: An apparatus for annealing alloy ribbon, the apparatus comprising: an unwinder unwinding an alloy ribbon from a spool of the alloy ribbon; a heating member comprising a first flat surface, on which the alloy ribbon unwound by the unwinder runs while contacting the first flat surface, the heating member heating the alloy ribbon running while contacting the first flat surface through the first flat surface; a cooling member comprising a second flat surface, on which the alloy ribbon heated by the heating member runs while contacting the second flat surface, the cooling member cooling the alloy ribbon running while contacting the second flat surface through the second flat surface; and a winder winding the alloy ribbon cooled by the cooling member.

Abstract: Provided is a hearth roll for supporting and conveying a steel sheet in a continuous annealing furnace, wherein all of a shaft portion and a roil main body are made from one or more ceramic materials, preferably constituted with concentric ceramic layers of different ceramic materials centering on the rotation shaft of the roll. The hearth roll has not only an excellent pickup resistance but also a long roll life free from maintenance for long periods. Also provided is a continuous annealing facility using the hearth roll in at least one of a heating zone, a soaking zone and a cooling zone.

Abstract: The processes can comprise feeding a furnace with a raw material. These materials can contain impurities and valuable metals (base metals, precious metals, platinum group metals, minor metals). The processes can allow the volatilization of arsenic and indium contained therein. Before volatilizing the material, composition of the material is optionally modified so as to obtain a ratio % S/(% (Cu/2)+% Ni+% Co) of about 0.5 to about 2. The processes can comprise feeding a melting device with the depleted material, and with a source of carbon in order to obtain a multi-layer product and an off gas. Before melting the depleted material, the depleted material composition is optionally modified so as to obtain a ratio % S/(% (Cu/2)+% Ni+% Co) of about 0.5 to about 2. Thus, it is possible to recover Cu, Ni and Co as well as several other metals, including In, Ge, Pb, Bi, precious metals and platinum group metals.

Abstract: Provided is a method for transporting reductant such as coke into a metallurgical furnace containing a bottom layer containing molten metal and a top layer on top of the bottom layer containing molten metal. The method comprises a first providing step for providing reductant, a second providing step for providing metal, a forming step for forming carriers containing reductant and metal of reductant provided in the first providing step and metal provided in the second providing step, and a feeding step for feeding carriers formed in the forming step into the metallurgical furnace. Also provided is a carrier and a production method for producing carriers.

Abstract: Embodiments of the present invention provide a die casting aluminum alloy, including the following components in percentage by mass: 11.0% to 14.0% of silicon; 0.1% to 0.9% of manganese; 0.1% to 1.0% of magnesium; 0.3% to 1.4% of iron; less than or equal to 0.2% of copper; and aluminum and inevitable impurities. The die casting aluminum alloy has good formability, heat conductivity, and corrosion resistance, and certain mechanical properties, which can avoid problems of a low yield of die-casting fittings, burn-in caused by severe heat emission of a product, corrosion in a coastal environment, assembly difficulties caused by insufficient mechanical properties, severe deformation in a wind load condition, and the like, so as to satisfy requirements of global delivery of complex communications products.

Abstract: A magnesium alloy suitable for use as a corrodible downhole article. The alloy has a corrosion rate of at least 50 mg/cm2/day in 15% KCl at 93° C. and a 0.2% proof strength of at least 50 MPa when tested using standard tensile test method ASTM B557-10.

Abstract: Embodiments described herein provide processes for forming and removing epitaxial films and materials from growth wafers by epitaxial lift off (ELO) processes. In some embodiments, the growth wafer has edge surfaces with an off-axis orientation which is utilized during the ELO process. The off-axis orientation of the edge surface provides an additional variable for controlling the etch rate during the ELO process and therefore the etch front may be modulated to prevent the formation of high stress points which reduces or prevents stressing and cracking the epitaxial film stack. In one embodiment, the growth wafer is rectangular and has an edge surface with an off-axis orientation rotated by an angle greater than 0° and up to 90° relative to an edge orientation of <110> at 0°.

Abstract: A method according to one embodiment includes combining an amorphous iron-based alloy and at least one metal selected from a group consisting of molybdenum, chromium, tungsten, boron, gadolinium, nickel phosphorous, yttrium, and alloys thereof to form a mixture, wherein the at least one metal is present in the mixture from about 5 atomic percent (at %) to about 55 at %; and ball milling the mixture at least until an amorphous alloy of the iron-based alloy and the at least one metal is formed. Several amorphous iron-based metal alloys are also presented, including corrosion-resistant amorphous iron-based metal alloys and radiation-shielding amorphous iron-based metal alloys.

Abstract: The disclosure relates to a process for producing a hardened and tempered component made of specially heat-treated cast iron (e.g., AGI). According to the disclosure, a main body made of cast iron is prepared which may already be in the shape of an engine block. The main body may then be subjected to pre-machining, which may include forming one or more bores. Then, the main body may be hardened and tempered by a suitable heat treatment, such as a special heat treatment. After the disclosed heat treatment, post-processing of the component may follow, such as establishing the final dimensions.

Abstract: The present invention provides a method for the fabrication of a steel sheet with a completely martensitic structure which has an average lath size of less than 1 micrometer and an average elongation factor of the laths is between 2 and 5. The elongation factor of a lath is defined as a maximum dimension 1max divided by and a minimum dimension 1max. The steel sheet has a yield stress greater than 1300 MPa and a mechanical strength greater than (3220(C)+958) megapascals. A composition of a semi-finished steel product includes, expressed in percent by weight, is, 0.15%?C?0.40%, 1.5%?Mn?3%, 0.005%?Si?2%, 0.005%?Al?0.1%, 1.8%?Cr?4%, 0%?Mo?2%, whereby: 2.7%?0.5 (Mn)+(Cr)+3(Mo)?5.7%, S?0.05%, P?0.1%, optionally: 0%?Nb?0.050%, 0.01%?Ti?0.1%, 0.0005%?B?0.005%, 0.0005%?Ca?0.005%. The semi-finished product is reheated to a temperature T1 in the range between 1050° C. and 1250° C., then subjected to a roughing rolling at a temperature T2 in the range between 1000 and 880° C.

Abstract: A high heat resistant steel with low nickel has high tensile strength and high heat resistance at a high temperature, wherein a value of X/Y is 0.44 to 0.47, and wherein the X is a value calculated by Equation 1, [Equation 1 is X=wt % of Cr+wt % of 1.5×Si+wt % of 0.5×Nb], and the Y is a value calculated by Equation 2 [Equation 2 is Y=wt % of Ni+wt % of 0.5×Mn+wt % of 30×C+wt % of 30×N].

Abstract: A steel sheet according to the present invention includes a predetermined chemical composition, in which amounts of each elements by mass % in the chemical composition satisfy both of expression “0.3000?{Ca/40.88+(REM/140)/2}/(S/32.07)” and expression “Ca?0.0058?0.0050×C”, and a number density of carbonitrides including Ti which exists independently and has a long side of 5 ?m or more is limited to 5 pieces/mm2 or less.

Abstract: A method of processing a non-magnetic alloy workpiece comprises heating the workpiece to a warm working temperature, open die press forging the workpiece to impart a desired strain in a central region of the workpiece, and radial forging the workpiece to impart a desired strain in a surface region of the workpiece. In a non-limiting embodiment, after the steps of open die press forging and radial forging, the strain imparted in the surface region is substantially equivalent to the strain imparted in the central region. In another non-limiting embodiment, the strain imparted in the central and surface regions are in a range from 0.3 inch/inch to 1 inch/inch, and there exists no more than a 0.5 inch/inch difference in strain of the central region compared with the strain of the surface region of the workpiece. An alloy forging processed according to methods described herein also is disclosed.

Abstract: Provided are a high-strength hot-dip galvanized steel sheet having excellent formability and a tensile strength of 440 MPa or more and a method for manufacturing the same. A steel sheet has a microstructure containing a ferrite phase having an area fraction of 60% or more, a pearlite phase having an area fraction of 20% to 30%, and a bainite phase having an area fraction of 1% to 5%, the area fraction of a cementite phase present in a grain of the ferrite phase being 5% or less. Upon manufacture, a hot-rolled sheet or a cold-rolled sheet is heated to a temperature of 650° C. or higher at an average heating rate of 10° C./s or more, is held at a temperature of 700° C. to (Ac3-5)° C. for ten seconds or more, is cooled to a temperature of 300° C. to 500° C. at an average cooling rate of 10° C./s to 200° C./s, is held at a temperature of 300° C. to 500° C. for 30 seconds to 300 seconds, and is then hot-dip galvanized.

Abstract: In one or more embodiments, a shielding device is provided to shield a bore of a shaft against surface treatment, the shielding device including a sleeve to be at least partially received within the bore, the sleeve defining on its side wall a through-aperture and being of a first cross-sectional dimension when the through-aperture is at a rest position, and a pin to be at least partially received within the sleeve, the sleeve being of a second cross-sectional dimension greater than the first cross-sectional dimension when the through-aperture is at an expanded position with the pin being at least partially received within the sleeve.

Abstract: The present invention provides a method for manufacturing a deposition mask, which irradiates laser light L to a resin film 20 to form an opening pattern 4 having a polygonal shape in a plan view, the method including a step of irradiating the laser light L that is shaped using a beam-shaping mask 10 having a light transmissive window 18 that allows the laser light L to pass therethrough with light transmittance gradually reducing with distance from an edge of the light transmissive window 18 on at least one of opposing sides thereof within an area outside the light transmissive window 18 to thereby form the opening pattern 4 having at least one pair of opposing side walls 4a that are inclined to open wide toward a surface of the film 20 to be irradiated with the laser light L, from a surface opposite to the irradiated surface.

Abstract: A mask frame assembly, an evaporation method and an array substrate are provided. The mask frame assembly according to the disclosure includes a frame, a covering mask and at least two evaporation masks, the evaporation masks are sequentially arranged on the frame with a gap between two adjacent evaporation masks. A first groove is arranged at a location corresponding to the gap in the frame. The first groove includes a first groove welding portion and a first groove weldless portion, the evaporation mask includes a first evaporation sub-mask corresponding to the first groove welding portion and a second evaporation sub-mask corresponding to the first groove weldless portion; and an edge of the first evaporation sub-mask and an edge of the first groove welding portion coincide.

Abstract: A method of growing carbon nanotubes is related. A reactor is provided. The reactor includes a reactor chamber and a carbon nanotube catalyst composite layer suspended in the reactor chamber. The carbon nanotube catalyst composite layer includes a carbon nanotube layer and a number of catalyst particles dispersed in the carbon nanotube layer. A mixture of carbon source gas and carrier gas is introduced into the reactor chamber to penetrate the carbon nanotube catalyst composite layer. The carbon nanotube catalyst composite layer is heated.

Abstract: An apparatus and a method for coating an inner wall of a metal tube are provided. The apparatus for coating an inner wall of a metal tube includes mounting posts on which both end openings of a metal tube are mounted and configured to block the inside of the metal tube from the ambient air so that a pressure in the metal tube is adjustable by the vacuum exhaust and inflow of process gases, a sputtering target metal tube installed inside the metal tube coaxially with the metal tube, a pulse electromagnet installed around an outside perimeter of the metal tube coaxially with the metal tube to apply a pulse magnetic field in an axial direction of the metal tube, an electromagnetic pulse power supply unit configured to apply pulse power to the pulse electromagnet, and a sputtering pulse power supply unit configured to synchronize a negative high-voltage pulse with the pulse power applied to the pulse electromagnet and apply to the sputtering target metal tube.

Abstract: A method of producing a Ni—P alloy sputtering target, wherein a Ni—P alloy having a P content of 15 to 21 wt % and remainder being Ni and unavoidable impurities is melted and atomized to prepare a Ni—P alloy atomized powder having an average grain size of 100 ?m or less, the Ni—P alloy atomized powder is mixed with a pure Ni atomized powder, and the obtained mixed powder is hot pressed. An object of the present invention is to provide a method of producing a Ni—P alloy sputtering target which achieves a small deviation from an intended composition.

Abstract: A chemical vapor deposition method comprises flowing a carrier liquid through a reactor. A fluid comprising one or more reactants is introduced into the carrier liquid. The fluid is at a first temperature and first pressure and is sufficiently immiscible in the carrier liquid so as to form a plurality of microreactors suspended in the carrier liquid. Each of the microreactors comprise a discrete volume of the fluid and have a surface boundary defined by an interface of the fluid with the carrier liquid. The fluid is heated and optionally pressurized to a second temperature and second pressure at which a chemical vapor deposition reaction occurs within the microreactors to form a plurality of chemical vapor deposition products. The plurality of chemical vapor deposition products are separated from the carrier liquid. A system for carrying out the method of the present disclosure is also taught.

Abstract: Fabrication method of a graphene film including the following successive steps: placing the substrate and a solid carbon source in a reaction chamber, provided with a gas inlet; and heating the solid carbon source, by flow of a current in said source, under a gas flow, the gas being devoid of hydrocarbon, so as to convert at least a part of the solid carbon source into a graphene film on the substrate.