Abstract: Described is a method for the production of D-erythrose and acetyl phosphate comprising the enzymatic conversion of D-fructose into D-erythrose and acetyl phosphate by making use of a phosphoketolase. The produced D-erythrose can further be converted into glycolaldehyde by a method for the production of glycolaldehyde comprising the enzymatic conversion of D-erythrose into glycolaldehyde by making use of an aldolase, wherein aldolase is a 2-deoxyribose-5-phosphate aldolase (EC 4.1.2.4) or a fructose-bisphosphate aldolase (EC 4.1.2.13). The produced glycolaldehyde can finally be converted into acetyl phosphate by the enzymatic conversion of the thus produced glycolaldehyde into acetyl phosphate by making use of a phosphoketolase or a sulfoacetaldehyde acetyltransferase.

Abstract: Provided are a novel modified ornithine decarboxylase protein having improved putrescine productivity and a use thereof.

Abstract: The invention provides compositions and methods for engineering bacteria to produce sialylated and N-acetylglucosamine-containing oligosaccharides, and the use thereof in the prevention or treatment of infection.

Abstract: The present invention provides a method for preparing a steviol glycoside and/or steviol comprising reacting glycosidase AOBGL1 and/or AOBGL3 or a variant thereof with a steviol glycoside having at least one monoglucoside bond and/or monoglucosyl ester bond, thereby cleaving said monoglucoside bond and/or monoglucosyl ester bond.

Abstract: A kit for detecting bacterial ATP in a sample is provided. The kit comprises an aqueous composition having a pH of about 6.0 to 7.2. The aqueous composition comprises effective amounts of a polyol, a buffer reagent, a protein, and ATP-diphosphohydrolase. A method of using the kit to detect bacterial ATP is also provided.

Abstract: A method for detecting lactic acid and/or acetic acid bacteria in a food-processing matrix is taught, using microbial flora including a lactic acid and/or acetic acid bacterial flora and a fungal flora. The bacterial flora contains an adenosine triphosphate of bacterial origin, and the fungal flora contains an adenosine triphosphate of fungal origin. The method comprises applying, to the matrix, before a first time limit, an antifungal having an antifungal action which is lethal, on the fungal flora, and at a second time limit, an antibiotic action which is non lethal, at a second time limit after the first time limit, on the bacterial flora. The microbial flora is detected between the first time limit and the second time limit; the lethal antifungal action releases, into the matrix, for the first time limit, adenosine triphosphate of fungal origin and in which the microbial flora is detected between the first time limit and the second time limit.

Abstract: A method is described to separate, characterize and/or identify microorganisms in a test sample. Said method comprises the steps of (i) lysing non-microorganism cells that might be present in the sample, (ii) separating the microorganisms as a pellet in the sample post-lysing non-microorganism cells, (iii) interrogating the pellet containing microorganisms by mass spectrometry to produce mass spectra of microorganisms, and (iv) characterizing and/or identifying microorganisms in the sample. The method may be used in characterizing/identifying microorganisms in complex samples, e.g., blood containing culture media.

Abstract: The present invention provides a high throughput method to quantify and characterize the size and integrity of viruses and viral molecules. In one embodiment, the present invention provides a method to quantify and characterize size and integrity of Foot and Mouth Disease virus (FMDV) using chromatographic system and in-line Dynamic Light Scattering (DLS) technique. In one embodiment, the present invention further comprises a column-switching system for running multiple analyses simultaneously. The present invention also provides a method to develop and evaluate FMDV containing products for preventing Foot and Mouth Disease (FMD). In one embodiment, the methods described herein assess the stability of FMDV. In another embodiment, the methods described herein serve as in-process quality control for a manufacturing process of FMD vaccine.

Abstract: Disclosed is a method for reducing measurement errors due to inhibition of catalase by azide in a method for quantification of a component to be measured, in which hydrogen peroxide derived from a component other than the component to be measured is decomposed by a catalase. The method for reducing measurement errors due to inhibition of catalase by azide employs a catalase which has a subunit having a molecular mass of 75 kDa or higher and is derived from a microorganism, when hydrogen peroxide derived from a component other than the component to be measured is decomposed by the catalase followed by quantification of hydrogen peroxide derived from the component to be measured to quantify the component to be measured.

Abstract: A method for monitoring resistance to a Bacillus thuringiensis pesticidal crystal protein is provided, which involves the measurement of protease activity or protease expression in the gut of adult insects.

Abstract: Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.

Abstract: The present disclosure relates to chemical compositions, kits, and apparatuses and methods for using these compositions, kits and apparatuses in various assays.

Abstract: The invention provides methods and processes for the identification of polymorphisms at one ore more designated sites, without interference from non-designated sites located within proximity of such designated sites. Probes are provided capable of interrogation of such designated sites in order to determine the composition of each such designated site. By the methods of this invention, one ore more mutations within the CFTR gene and the HLA gene complex can be identified.

Abstract: The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCl, pH 8.5 at 25° C., 50 mM KCl and 5 mM MgCl2; (ii) is substantially specific for single stranded DNA; and (iii) has a 3?-5? exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used.

Abstract: The present invention is directed to a method of detecting a genomic rearrangement in a nucleic acid sample with Long Insert Whole Genome Sequencing (LI-WGS). The method may include obtaining a nucleic acid sample and then fragmenting the nucleic acid sample (e.g., via sonication). In particular, the fragmenting may result in the production of a plurality of inserts. Thereafter, the method comprises purifying the plurality of inserts using magnetic beads and then amplifying the purified plurality of inserts. In addition, the method further comprises sequencing the purified and amplified plurality of inserts. In some aspects, the plurality of inserts have a length of between about 800 and about 1,100 base pairs.

Abstract: A multiplex slide plate device and an operation method thereof are provided. The multiplex slide plate device includes a slide plate and a sacrificial layer. The slide plate has reaction vessels arranged in an array, wherein each of the reaction vessels has an opening portion and a bottom portion. The sacrificial layer has a microfluidic channel, wherein the microfluidic channel has an injection channel, a main channel and a distal channel connected to each other. The sacrificial layer is assembled to the slide plate, wherein the main channel faces the opening portion. A sample solution is injected into the injection channel, such that the sample solution flows from the injection channel through the main channel to the distal channel, wherein the sample solution loads into each of the reaction vessels while flowing through the main channel.

Abstract: A nucleotide sequence having SEQ ID NO:1, a universal reverse primer having SEQ ID NO:2, a universal RT primer, a method for designing primer, and a miRNA detection method are provided. In the miRNA detection method, the universal RT primer is used for the cDNA synthesis of a miRNA sample and the universal reverse primer is used for cDNA molecule amplification in qPCR quantitative detection. The universal reverse primer sequence, the nucleotide sequence, and the design rules are used in the method for designing primer, so as to design a primer for qPCR quantitative detection.

Abstract: In one embodiment, a polymerase chain reaction (PCR) system includes a mixture chamber, a denature chamber, an annealing chamber, an extension chamber, and a product chamber, that are fluidically coupled to one another through a plurality of microfluidic channels. An inertial pump is associated with each microfluidic channel, and each inertial pump includes a fluid actuator integrated asymmetrically within its associated microfluidic channel. The fluid actuators are capable of selective activation to circulate fluid between the chambers in a controlled cycle.

Abstract: Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template nucleic acid, a template switch oligonucleotide, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template nucleic acid and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid including a region polymerized from the dNTPs by the polymerase. The methods further include attaching sequencing platform adapter constructs to ends of the product nucleic acid or a derivative thereof. Aspects of the invention further include compositions and kits.

Abstract: Disclosed are processes that use DNA polymerases to extend primers annealed to templates, wherein a standard nucleotide in the template guiding the extension directs the incorporation of a nonstandard nucleotide analog into the product duplex opposite that standard template nucleotide, and processes wherein a nonstandard nucleotide in the template guiding the extension directs the incorporation of a standard nucleotide analog opposite the nonstandard template nucleotide. Conditions, including the pH of the mixture where the primer extension is done and the composition of triphosphate mixtures where the primary extensions done, are disclosed.

Abstract: The invention provides a method for determining copy number variations (CNV) of a sequence of interest in a test sample that comprises a mixture of nucleic acids that are known or are suspected to differ in the amount of one or more sequence of interest. The method comprises a statistical approach that accounts for accrued variability stemming from process-related, interchromosomal and inter-sequencing variability. The method is applicable to determining CNV of any fetal aneuploidy, and CNVs known or suspected to be associated with a variety of medical conditions. CNV that can be determined according to the method include trisomies and monosomies of any one or more of chromosomes 1-22, X and Y, other chromosomal polysomies, and deletions and/or duplications of segments of any one or more of the chromosomes, which can be detected by sequencing only once the nucleic acids of a test sample.

Abstract: A method for sequencing a nucleic acid template includes forming a nanowire assembly including a semiconductor nanowire and a probe covalently bound to the semiconductor nanowire; contacting the nanowire assembly with a template nucleic acid; contacting the nucleic acid duplexes with an extension nucleic acid, the extension nucleic acid joined to the probe; disrupting the nucleic acid duplexes; and measuring an electrical characteristic of a nanowire assembly of the set of nanowire assemblies.

Abstract: This disclosure provides systems and methods for sample processing and data analysis. Sample processing may include nucleic acid sample processing and subsequent sequencing. Some or all of a nucleic acid sample may be sequenced to provide sequence information, which may be stored or otherwise maintained in an electronic storage location. The sequence information may be analyzed with the aid of a computer processor, and the analyzed sequence information may be stored in an electronic storage location that may include a pool or collection of sequence information and analyzed sequence information generated from the nucleic acid sample. Methods and systems of the present disclosure can be used, for example, for the analysis of a nucleic acid sample, for producing one or more libraries, and for producing biomedical reports. Methods and systems of the disclosure can aid in the diagnosis, monitoring, treatment, and prevention of one or more diseases and conditions.

Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

Abstract: Methods for diagnosing or monitoring endometriosis in a mammal are provided. The methods include the steps of determining the expression levels of BDNF and its receptor, Ntrk2, in a biological sample from the mammal, and determining that the mammal has endometriosis when the BDNF and Ntrk2 expression levels in the sample are elevated.

Abstract: A risk stratification method for a patient in a disease state and specifically patients presenting a tumor, includes determining if the patient is a homozygote or heterozygote and further determining the allelic expression for the patient, CC, T/C, or C/T. For patients having the cytosine methylated, they have a TIC allelic expression and patients without a methylated cytosine have a C/T allelic expression. A patient with a TT allelic expression is classified as a highest risk patient, a patient with a TIC allelic expression is classified as a second highest risk patient, a patient with a C/T allelic expression is classified as a third highest risk patients and a patient with a CC allelic expression is classified as a lowest risk patient. The risk stratification method may further include identification of an abnormal expression or mutation/function of a gene product produced by CTCF binding site 6.

Abstract: The invention pertains to increased LINC00473 as an indicator of a cancer involving loss or reduction in LKB1 function. LINC00473 is also provided as a therapeutic target for treating a cancer involving loss or reduction in LKB1 function. The invention provides a method of identifying a subject as having a cancer involving loss or reduction in LKB1 function based on the level of LINC00473 in the test sample obtained from the subject and administering an effective amount of a LINC00473 inhibitor to the subject to treat the cancer. The LINC00473 inhibitor can be a small-inhibitory RNA, short hairpin RNA, bifunctional RNA, antisense oligonucleotide, ribozyme, deoxyribozyme, aptamer or small molecule inhibitor. A pharmaceutical composition comprising a LINC00473 inhibitor is also provided for the treatment of a cancer involving loss or reduction in LKB1 function.

Abstract: An exemplary embodiment describes a method for detection of bacteria and fungi in a sample of biological material, wherein the DNA contained in the sample of biological material is subjected to amplification in multiplex real-time PCR, with the use of primers specific for bacteria in the first stage and primers specific for fungi, and in the second stage, the resulting DNA is amplified using primers and probes differentiating fungi into a group of mold fungi and yeast fungi and bacteria into Gram-positive and Gram-negative bacteria. Another exemplary embodiment refers to oligonucleotide primers for the detection of bacteria and fungi by PCR and a kit for simultaneous detection of bacteria and fungi.

Abstract: The present invention provides methods for the detection of cyanobacteria, and in particular, methods for the detection of cyanotoxic organisms. The invention further relates to methods of screening for compounds that modulate the activity of polynucleotides and/or polypeptides of the saxitoxin biosynthetic pathways.

Abstract: The present invention relates to nucleic acids and methods for detecting pathogens and beneficial microorganisms.

Abstract: Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of a 16S rRNA or its encoding gene from bacterial species associated with bacterial vaginosis. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.

Abstract: The invention relates to methods for detecting target DNA sequences, particularly for detecting Leishmania infection, comprising a recombinase-polymerase isothermal amplification and detection by electrochemistry. The invention also relates to kits and oligonucleotides for performing said methods.

Abstract: The present disclosure is in the field of plant breeding and disease resistance. The disclosure provides methods for breeding canola plants having clubroot resistance using marker-assisted selection. The disclosure further provides germplasm resistant to various Plasmodiophora brassicae pathotypes including pathogtype 5x. The disclosure also provides markers associated with clubroot resistance loci for introgressing these loci into elite germplasm in a breeding program, thus producing novel clubroot resistant germplasm, e.g., spring canola varieties resistant to pathogtype 5x.

Abstract: Methods that may be used for the electrochemical detection of multiple parameters, including chemical compounds. Further provided are cells that may be used in the electrochemical detection of multiple parameters, including chemical compounds, as well as a kit for the electrochemical detection of multiple parameters, including chemical compounds.

Abstract: Provided herein are a GeXP rapid detection primer set for simultaneously identifying gene HA of eight different human-infected subtypes of avian influenza virus, a kit, and use thereof. Disclosed are 9 pairs of specific primer and 1 pair of universal primer, for a GeXP rapid detection primer kit for simultaneously identifying gene HA of eight different human-infected subtypes of avian influenza virus. Eight different human-infected subtypes HA of avian influenza virus from the nine genes of M, H1, H2, H3, H5, H6, H7, H9 and H10 can be identified simultaneously with a sensitivity of 102 copies/?L.

Abstract: The present invention relates generally to an assay for detecting and differentiating multiple analytes, if present, in a single fluid sample, including devices and methods therefore.

Abstract: An embodiment of a method for diagnostic testing includes: providing a sampling kit to a subject, the sampling kit including a sample container for reception of a sample from a collection site of the subject; receiving the sample from the subject; generating a microbiome sequence dataset based upon sequencing nucleic acid content of a microorganism portion of the sample; detecting a presence of a set of microbiome targets; generating a diagnostic analysis based on the detected set of microbiome targets; generating a therapy recommendation based on the set of microbiome targets; and promoting the therapy recommendation in coordination with presenting information derived from the diagnostic analysis.

Abstract: Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of a human papillomavirus (HPV) nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.

Abstract: A detection wave from a transmitting/receiving means is guided to the interior of a blast furnace via an antenna and a reflecting plate, and when a reflected wave from the surface of a loaded material is reflected by the reflecting plate and received by the transmitting/receiving means, the reflecting plate is rotated together with the antenna, or the reflecting plate is rotated additionally, and the surface profile of the loaded material is measured by scanning the surface of the loaded material in a linear manner or a planar manner during the turning of a chute or for each prescribed turn of the chute. A deposition profile is obtained on the basis of this surface profile and is compared to a predetermined theoretical deposition profile, and the chute is controlled so as to correct the error with respect to the theoretical deposition profile and then which new loaded material is introduced.

Abstract: The present invention relates to the steel alloy for railway components and the process of manufacturing it, said alloy comprising, in weight percentage, from 0.21 to 0.27 carbon, from 0.80 to 1.20 manganese, from 0.35 to 0.60 silicon, up to 0.02 phosphorus, up to 0.02 sulfur, from 0.55 to 0.65 chromium, from 0.45 to 0.55 molybdenum, from 1.75 to 2.05 nickel and from 0.005 to 0.030 titanium, conferring, with its composition, desirable mechanical properties, hardenability and corrosion resistance for application on railway components.

Abstract: A workpiece for an induction hardening is provided. The workpiece has a first inclined surface, a second inclined surface, and a connecting surface connecting the first inclined surface and the second inclined surface on a side toward which the first inclined surface and the second inclined surface approach each other. The connecting surface has a recessed portion. A hardened layer formed at the first inclined surface and another hardened layer formed at the second inclined surface do not overlap each other at the recessed portion.

Abstract: A method of manufacturing a hollow stabilizer includes a forming step of subjecting an element pipe to a bending process, to form a product shape including bent portions, and a quenching step of quenching the element pipe subjected to the bending process. In the quenching step, a cooling process is performed by immersing the element pipe made of steel in coolant and by spraying the coolant to an outer surface of the bent portion.

Abstract: Provided are a high-strength steel sheet for containers and a method for producing the high-strength steel sheet. The high-strength steel sheet for containers has a composition containing, by mass, C: 0.0010% to 0.10%, Si: 0.04% or less, Mn: 0.10% to 0.80%, P: 0.007% to 0.100%, S: 0.10% or less, Al: 0.001% to 0.100%, N: 0.0010% to 0.0250%, and the balance being Fe and inevitable impurities. The difference between the dislocation density at the uppermost layer of the high-strength steel sheet in the thickness direction and the dislocation density at a depth of ¼ of the thickness of the high-strength steel sheet from the surface is 1.94×1014 m?2 or less. The high-strength steel sheet has a tensile strength of 400 MPa or more and a fracture elongation of 10% or more.

Abstract: A method for producing a high strength steel piece having desired mechanical properties, obtainable by a reference heat treatment comprising a first reference treatment and a final reference treatment comprising at least an overaging, The method comprising a step of heat treating on an equipment comprising at least an overaging means for which it is possible to set at least one operating point, the final treatment comprising an overaging for which it is possible to calculate two final treatment parameters OAP1 and OAP2 depending on an operating point of the overaging means. A minimum OAP1 min and a maximum OAP2 max final treatment parameters are determined in order to obtain the desired properties, at least one operating points of the overaging section means is determined such that OAP1?OAP1 min and OAP2?OAP2 max. The piece is heat treated accordingly. The parameters are with T (t) the temperature at QT time t Formula (I).

Abstract: An apparatus for continuously treating metal strip of aluminum, an aluminum alloy, a nonferrous metal, or a nonferrous-metal alloy, has at least one heat-treatment device through which the metal strip passes in a strip-travel plane in a travel direction without contact from an upstream inlet end to a downstream outlet end and having a heating zone at the upstream end and a cooling zone formed by a row extending in the direction of at least two cooling subzones. A strip-centering device between the cooling subzones adjusts a position of the metal strip in the strip-travel plane and transverse thereto.

Abstract: A wire heating system includes an induction heating apparatus having a power supply and an induction coil arranged to heat a wire rod by an induction heating using current supplied from the power supply, and a controller configured to control the current to be supplied to the induction coil based on a feeding speed of the wire rod. The induction heating apparatus has a heating section in which the wire rod is heated by the induction heating using the induction coil, and a soaking section located downstream of the heating section to homogenize the temperature distribution of the induction-heated wire rod. The controller is configured to control the current to be supplied to the induction coil such that a temperature of the wire rod at a downstream end of the soaking section becomes a target temperature.

Abstract: A continuous annealing system and a continuous annealing method with which it is possible to achieve an annealing atmosphere having a low dew point which is suitable for annealing a steel strip containing easily oxidizable metals such as Si and Mn at low cost and with stability by preventing easily oxidizable metals such as Si and Mn in steel from being concentrated in a surface portion of a steel strip and preventing the formation of oxides of easily oxidizable metals such as Si and Mn.

Abstract: A method and system are provided in which a gold and/or silver-collecting resin-in-leach circuit comprises both co-current and counter-current sections.

Abstract: The present invention relates to a method for recovering vanadium and tungsten from a leach solution of a waste denitrification catalyst, and more specifically, to a method for recovering vanadium and tungsten from a leach solution of a waste denitrification catalyst comprising the steps of: recovering vanadium by adding acid and then adding a calcium compound to a leach solution of a waste denitrification catalyst to precipitate the vanadium; and recovering tungsten by adding acid and then adding a calcium compound to the remaining leach solution after recovering the vanadium to precipitate the tungsten.

Abstract: An aluminum alloy plate includes peritectic elements and Mg. Wherein plate thickness of the plate is represented as t (mm), a range within ±0.01×t from t/2 is represented as a central portion, a range within ±0.01×t from t/4 is represented as a quarter portion, and a range within 0.02×t from a top surface in the plate thickness direction is represented as a superficial portion, concentration of the peritectic elements is such that a concentration difference between in the central portion and in the quarter portion, and a concentration difference between in the central portion and in the superficial portion are 0.04% (mass %) or less. In addition, concentration of the Mg is such that a concentration difference between in the central portion and in the quarter portion, and a concentration difference between in the central portion and in the superficial portion of the plate thickness are 0.4% or less.