Abstract: The present invention relates to an improved CuAl2O4 spinel based catalyst additive composition having bi-modal pore size for improving gasoline sulfur removal activity by maintaining high gasoline selectivity and maintaining research octane number (RON) while cracking heavier hydrocarbon feedstocks in the fluid catalytic cracking unit. More particularly, present invention relates to a gasoline sulfur reduction (GSR) additive comprising copper aluminate spinel, acidic alumina matrix; and clay, wherein the additive having bimodal pore distribution. Present invention also relates to a process for preparing the gasoline sulfur reduction (GSR) additive.

Abstract: A process is presented to treat a process stream containing a hydrocarbon (oil and/or gas) and hydrogen sulfide with a liquid treatment solution containing a sulfur dye catalyst. The process stream can be within a pipeline, wellbore, subsea pipeline or a wellhead that contains hydrogen sulfide where the liquid treatment solution is injected at a predetermined point to define a scavenger zone such that the sulfur dye catalyst in the liquid treatment solution causes the sulfide from the hydrogen sulfide to react with the catalyst. The hydrocarbon component is separated substantially free of the hydrogen sulfide from a spent treatment solution containing spent sulfur dye catalyst which can then be fed to an oxidation vessel where it is contacted with an oxygen containing gas causing the sulfide to oxidize to thiosulfate and converting the spent sulfur dye catalyst to regenerated sulfur dye catalyst.

Abstract: The present invention relates to a method and system for treating a flow back fluid exiting a well site following stimulation of a subterranean formation. More specifically, the invention relates to processing the flow back fluid, and separating into a carbon dioxide rich stream and a carbon dioxide depleted stream, and continuing the separation until the carbon dioxide concentration in the flow back stream until the carbon dioxide concentration in the flow back gas diminishes to a point selected in a range of about 50-80 mol % in carbon dioxide concentration, after which the lower concentration carbon dioxide flow back stream continues to be separated into a carbon dioxide rich stream which is routed to waste or flare, and a hydrocarbon rich stream is formed.

Abstract: A process for facile separation of lighter hydrocarbon fractions from the heavier fractions of hydrocarbon oil feedstocks is disclosed, which utilizes novel sparging and reverse distillation techniques. The present invention can be utilized for the facile “topping” of crude oil extracted on-site. Moreover, while heavier hydrocarbon fractions may be shipped to refineries for further processing, this invention will also prove useful for quick separation of light fractions produced by cracking processes off-site.

Abstract: Provided are embodiments that include a hydrocarbon fluid processing system including an ultrasonic hydrocarbon degassing unit including a vapor recovery vessel adapted to direct flow of a hydrocarbon fluid mixture along a flowpath extending through an interior of the vapor recovery vessel, and an ultrasonic transducer system disposed inside the vapor recovery vessel and in the flowpath of the hydrocarbon fluid mixture. The hydrocarbon fluid mixture including a hydrocarbon liquid and a gas entrained in the hydrocarbon liquid, the ultrasonic transducer system adapted to transmit ultrasonic signals into the hydrocarbon fluid mixture along the flowpath, and the ultrasonic signals adapted to separate the gas from the hydrocarbon liquid.

Abstract: The invention relates to a reforming catalyst. The reforming catalyst comprises a reforming metal, such as Pt, a support, such as an alumina support, and a USY zeolite, which has had part of its aluminum framework substituted with Zr and Ti. The amount of USY zeolite does not exceed 5 wt %, and most preferably, contains 2-3 wt % USY zeolite.

Abstract: The present invention relates to the field of inhibition of metal corrosion in hot acidic hydrocarbons, wherein acidity is derived from presence of naphthenic acid. More particularly, it relates to a polymeric additive for inhibiting high temperature napthenic acid corrosion, wherein said polymeric additive is polymeric phosphate ester of polyisobutylene succinate ester or oxide derivative of polymeric phosphate ester of polyisobutylene succinate ester. A polymeric phosphate ester of polyisobutylene succinate ester which is capable of acting as naphthenic acid corrosion inhibitor by inhibiting naphthenic acid corrosion in crude oil/feedstock/hydrocarbon streams containing naphthenic acid, and demonstrating higher thermal stability at elevated temperature varying from about 200° C. to about 400° C. [about 400° F. to about 750° F.] is disclosed.

Abstract: The invention provides a method of biomass grading pyrolysis gasification in a circulating fluidized bed comprising: feeding biomass into the lower-middle part of a carrying fluidized bed, mixing with high temperature synthesis gas and heat carrier from a turbulent fluidized bed, heating the biomass to carry out a pyrolysis reaction, and carrying the pyrolysis product upward; subjecting the cracked oil and gas to a gaseous phase catalytic cracking in an upper-middle part of the carrying fluidized bed, cracking the tar into methane, ethane and the like; subjecting the heat carrier, semi-coke and fuel gas after the reaction to the multi-stage of gas-solid separation, a large particle carrier and semi-coke following a first-level separation are used as the fuel gas cracking catalyst and the filter material for filtering and removing dust, and enter into a moving bed filter to separate out an ultra-fine ash and subsequently return to the turbulent fluidized bed so as to perform gasification reaction, the ultra-fi

Abstract: A lubricant composition comprising one or several poly-alpha olefin homopolymer(s), a suspension of particles having a size between 0.5 ?m and 25 ?m and an additive, and uses thereof in the glass industry, such as in the production of hollow glasses for the packaging of products to be applied on mammals.

Abstract: The invention provides a lubricating composition containing an oil of lubricating viscosity, an ashless thiocarbamate compound having an optionally-substituted hydrocarbyl group on an S-atom and an optionally-substituted hydrocarbyl group on an N-atom, and a boron-containing compound. The invention further relates to a method of lubricating an internal combustion engine with the lubricating composition.

Abstract: The invention relates to low molecular weight hydrogenated polybutadienes and to a method for the preparation of these polymers. The invention is also directed to lubricant compositions comprising these polymers and uses thereof.

Abstract: A solid-state lubricant composition is disclosed. The solid-lubricant contains graphene, an oxide of a metal, and one or more polymeric binders. A method of making a solid-state lubricant coating on various substrates is disclosed. The method includes the steps of making a homogeneous slurry comprising powder of an oxide of a metal, graphene, and a polymeric binder with organic volatile solvent; coating a substrate with the homogeneous slurry with desired thicknesses; and drying the slurry on the substrate naturally or applying additional heat, resulting in a solid lubricant coating on the substrate. Substrates with coated solid composite lubricant show wear reduction and lower coefficient of friction compared with uncoated substrates.

Abstract: Provided here is an enzymatic process for extraction of oil from algae biomass. The oils produced by the processes herein are used in animal feed and human consumption.

Abstract: A system and method for the separation of nut or seed oil from shelled nuts or seeds are disclosed. The method comprises the grinding of shelled nuts or seeds and at least one carbohydrate for a length of time sufficient to promote nut or seed oil separation, leaving behind defatted nut or seed dough that can be further processed into a food product such as snack bar or nut milk. A system for the separation of nut or seed oil from shelled nuts or seeds comprises sources for both shelled nuts or seeds and at least one carbohydrate, and a grinding unit comprising at least one movable member, optional stationary member, and a vessel.

Abstract: The present invention relates to trans isomers of formula (I) in the form of any one of its stereoisomers or a mixture thereof, and wherein the bold and hatched lines indicate a relative or absolute configuration; and one dotted line represents a carbon-carbon single bond and the other a carbon-carbon double bond; and their uses as perfuming ingredients (e.g. to impart vetiver/rooty notes), e.g. in consumer products.

Abstract: The present disclosure is directed to non-corrosive cleaning compositions that are useful, e.g., for removing residues (e.g., plasma etch and/or plasma ashing residues) and/or metal oxides from a semiconductor substrate as an intermediate step in a multistep manufacturing process.

Abstract: A composition including a plurality of particles, the particles including: about 25% to about 94% by weight a water-soluble carrier; about 5% to about 45% by weight of a branched polyester; and an optional deposition aid; wherein each of the particles can have a mass from about 1 mg to about 1 g.

Abstract: Provided are various methods and systems for producing peroxycarboxylic acid compositions, and in particular, nonequilibrium compositions of peracetic acid. The methods and systems control flow rates and proportions of feedstocks/reactants, perform the required sequence of reaction steps to produce high yield peroxycarboxcylic acid solutions in a continuous manner, and provide optimal reaction time and reactant mixing for continuous and safe on-site production.

Abstract: The invention combines chemical cleaning with mechanical cleaning. In case of large scale facilities, the invention allows cleaning from bottom to top which obviates the need for special arrangements such as confined space entry scaffolding and safety nets removal in order to reach higher or inner parts. The dual effect of the combined mechanical and chemical cleaning facilitates a surprising efficient removal of organic and inorganic sediments. The chemical cleaning is achieved by a reaction of cleaning chemicals with the organic matter such as: oils, greases, hydrocarbons, cellulose. The implementation of the invention permits use of non-toxic, non-hazardous, environmental friendly mixture of sodium carbonate and sodium bicarbonate. Typical particle size of the thin powder used according to a preferred embodiment of the present invention shall be between 20-100 ?m.

Abstract: A cleaning solvent composition comprises from about 70 to about 95.7 weight percent trans-dichloroethylene, from about 15 to about 3.8 weight percent heptafluorocyclopentane, and from about 15 to about 0.5 weight percent of methylperfluoroheptene ethers. The composition may comprise from about 88 to about 94.2 weight percent trans-dichloroethylene, from about 6 to about 3.8 weight percent heptafluorocyclopentane, and from about 6 to about 2 weight percent of methylperfluoroheptene ethers. A method for cleaning articles of soiling substances comprises contacting the articles with the solvent composition by any suitable means such as a spray delivered by a propellant gas, or by contact with liquid and/or vapor solvent compositions, as in a conventional vapor degreaser apparatus.

Abstract: A liquid transport method is disclosed for efficiently producing cell cultures. The liquid transport method of the present disclosure, which is in processing of cells, includes: a) a step in which a container holding a liquid is gripped by a gripping tool of a robot; and b) a step in which the liquid in the container is transported to a collection container by rotating the gripped container. In steps a) and b) the robot operates such as not to pass over a vertical line of an opening of the collection container.

Abstract: Equipment for fermentation of beverages, particularly kombucha, that limits alcohol production during fermentation, increases throughput by reducing fermentation times, and utilizes production plant floorspace efficiently. Unlike traditional fermentation equipment that uses large, often cylindrical, vats, embodiments of the invention use vertical stacks of relatively shallow fermentation trays. The shallow trays increase the ratio of surface area to liquid volume, thereby improving oxygen flow to the fermenting liquid; increased oxygen flow reduces alcohol production for kombucha and reduces fermentation time. Air gaps between trays in the vertical stack, and headspace between the top of the fermenting liquid and the tray top edges, are optimized to promote oxygen flow. Fermentation trays are stacked vertically to maintain high production capacity with limited facility floorspace.

Abstract: A method for expanding circulating tumor cell in vitro includes preparing a cell culture tool having a multi-particle colloidal crystal layer, preparing a cell solution including circulating tumor cells, and contacting the cell solution with the multi-particle colloidal crystal layer, to attach the circulating tumor cells in the cell solution to the multi-particle colloidal crystal layer and rapidly expand by 20 times or more. The multi-particle colloidal crystal layer at least includes first particles having a particle size of 1000 to 5000 nm and second particles having a particle size of 20 to 400 nm. The culture medium in the cell solution at least includes a platelet lysate.

Abstract: The present invention relates to a non-powered constant-temperature cell transfer device, in which a first container, which accommodates living animal cells and a culture solution, is accommodated in a second container having a heat generating unit that emits heat generated by oxidation and reduction reactions of metal by introducing oxygen in the atmosphere in order to continuously provide an optimum culture temperature to a culture container even without being supplied with electric power, thereby maintaining activity and viability of the cells by maintaining a culture environment optimal for proliferation of the cells accommodated in the first container.

Abstract: Discloses is a continuous fermenter for sequential fermentation of hexose and pentose which includes (a) a hexose fermenter equipped with a saccharified solution supply unit containing hexose, pentose and lignin, a plurality of trays closing at least half of the diameter of the fermenter, impellers disposed on each of the trays, an impeller driving unit, a lignin discharge unit disposed at the bottom of the fermenter, a fermented solution discharge unit, and a temperature control jacket; and (b) a pentose fermenter equipped with a fermented solution supply unit for supplying the fermented solution discharged from the hexose fermenter, a plurality of trays closing at least half of the diameter of the fermenter, impellers disposed on each of the trays, an impeller driving unit, a lignin discharge unit disposed at the bottom of the fermenter, a fermented solution discharge unit, and a temperature control jacket.

Abstract: A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

Abstract: Disclosed herein are systems, methods, and devices for determining plant-microbe interactions. In some embodiments, the device comprises: a root chamber configured to allow a root of a plant to grow, wherein the root chamber is connected with: an inlet channel for introducing a medium into the root chamber, an outlet channel for collecting plant exudates and metabolites, and a plant reservoir for the plant shoot of the plant to grow.

Abstract: Methods are provided for the generation of mesodermal cell types and derivatives thereof. Also provided are methods for generating purified populations of mesodermal cell types and derivatives thereof. The instant disclosure also provides methods of screening for cellular responses of the generated mesodermal cell types and derivatives thereof. Also provide are methods for screening for organismal phenotypes induced by introduction of the generated mesodermal cell types and derivatives thereof. Treatment methods making use of the generated mesodermal cell types and derivatives thereof are also provided. The instant disclosure also provides systems, compositions, and kits for practicing the methods of the disclosure.

Abstract: The present invention features compositions and methods related to the isolation, culture and therapeutic use of CD31-expressing cells.

Abstract: According to the present invention, there are provided a method for producing a human T cell, which comprises the steps of inducing an iPS cell from a human T cell, and differentiating the iPS cell into a T cell; a pharmaceutical composition comprising the T cell produced by the method; and a method for cell-based immunotherapy using the method.

Abstract: The present invention relates to methods and kits for expanding a stem cell population. More particularly, the invention relates, inter alia, to methods, kits, and compositions for expanding a stem cell population, particularly a hematopoietic stem cell population.

Abstract: Methods and compositions for rapid development of reporter lines utilizing safe harbor sites in iPSCS, as well as other progenitor cells, pluripotent and multipotent stem cells and differentiated cells, and multiple Lox sites are provided.

Abstract: The present invention relates to the fields of life sciences and medicine. Specifically, the invention relates to cancer therapies of humans. More specifically, the present invention relates to oncolytic adenoviral vectors alone or together with therapeutic compositions for therapeutic uses and therapeutic methods for cancer. In one aspect the present invention relates to separate administration of adoptive cell therapeutic composition and oncolytic adenoviral vectors. Furthermore, the present invention relates to a pharmaceutical kit and a pharmaceutical composition, both utilizing oncolytic adenoviral vectors.

Abstract: Embodiments of the disclosure concern systems, methods, and/or compositions for cultivation of mammalian viruses, including at least human noroviruses and sapoviruses within the Caliciviridae family of viruses. The ex vivo culture systems include intestinal enteroids in combination with bile or a functionally active fraction or component thereof. In specific embodiments, the culture system is utilized to test inactivation compounds for therapeutic or environmental efficacy and to test contaminated comestibles and/or environmental entities for determination of the presence of infectious virus. Furthermore, antiviral compositions may be tested using systems of the disclosure, including drugs, small molecule inhibitors, and biologies such as neutralizing monoclonal antibodies.

Abstract: Provided are compositions related to HSD17B13 variants, including isolated nucleic acids and proteins related to variants of HSD17B13, and cells comprising those nucleic acids and proteins. Also provided are methods related to HSD17B13 variants. Such methods include methods for modifying a cell through use of any combination of nuclease agents, exogenous donor sequences, transcriptional activators, transcriptional repressors, and expression vectors for expressing a recombinant HSD17B13 gene or a nucleic acid encoding an HSD17B13 protein. Also provided are therapeutic and prophylactic methods for treating a subject having or at risk of developing chronic liver disease.

Abstract: The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for expressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.

Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.

Abstract: The invention relates to provision of artificial bioluminescent enzymes. The invention provides for a polypeptide, including any one of amino acid sequences (i) to (iii) below, and having luciferase activity: (i) an amino acid sequence of SEQ ID NO: 1; (ii) an amino acid sequence of SEQ ID NO: 1 in which 1 to 10 amino acids are substituted, added, or deleted; or (iii) an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 1.

Abstract: A Bradyrhizobium monooxygenase, a gene for encoding the monooxygenase, a recombinant expression vector comprising the gene and a recombinant transformant, a method of preparing the monooxygenase by the recombinant expression transformant, and a method of preparing an optically pure chiral sulfoxide by the monooxygenase, in particular to a method of preparing prazole drugs by means of catalyzing the asymmetric oxidation of thioether, a prazole precursor. As compared with other methods of preparing an optically pure sulfoxide, the product produced by the monooxygenase of the present invention as a catalyst has high optical purity, avoids the generation of the byproduct sulfone, and has advantages of mild reaction conditions, simple and convenient operations, easy amplification, etc.

Abstract: In one aspect, the invention relates to an immunogenic composition that includes a mutant Clostridium difficile toxin A and/or a mutant Clostridium difficile toxin B. The mutant toxin may include a glucosyltransferase domain having at least one mutation and a cysteine protease domain having at least one mutation, relative to the corresponding wild-type C. difficile toxin. The mutant toxins may include at least one amino acid that is chemically crosslinked. In another aspect, the invention relates to methods and compositions for use in culturing Clostridium difficile and in producing C. difficile toxins.

Abstract: The present invention relates to DNA polymerases. In particular, the present invention relates to DNA polymerases based on a DNA polymerase from a Psychrobacillus sp. The present invention provides an isolated DNA polymerase or an enzymatically active fragment thereof, said DNA polymerase comprising the amino acid sequence of SEQ ID NO:1 or comprising an amino acid sequence which is at least 70% identical to SEQ ID NO:1. The invention also provides nucleic acid molecules comprising a nucleotide sequence that encodes the DNA polymerase. The invention also provides a method of nucleotide polymerisation and a method of amplifying a nucleic acid in which the DNA polymerase or an enzymatically active fragment thereof is used.

Abstract: The present invention is directed to methods and compositions for genome editing and DNA targeting of proteins.

Abstract: A process for producing oligosaccharides from lignocellulosic biomass, having the following steps: a) pre-treating the biomass in a pre-treatment reactor (1) in order to provide an effluent containing a pre-treated substrate; b) enzymatic hydrolysis of the pre-treated substrate contained in the effluent from step a) in a reactor, in the presence of cellulases such that a hydrolysate containing glucose, cellulases and water is produced; c) removing at least a portion of the hydrolysate from step b) comprising a liquid fraction; d) reducing the water content of said portion of the hydrolysate in step c) such that the liquid fraction of the hydrolysate has a water content of less than 65% by weight with respect to the total weight of the liquid fraction; e) incubating the hydrolysate from step d) at a temperature in the range 40° C. to 70° C. for the time necessary to produce an effluent enriched in oligosaccharides.

Abstract: The invention is directed to novel alkaline proteases.

Abstract: In various embodiments methods are provided for delivering an agent of interest (e.g., protein, antibody, nucleic acid) into cells. In certain embodiments the method comprises contacting the cells with anisotropic magnetic particles in the presence of the agent; and applying a substantially uniform magnetic field to said magnetic particles where movement of said particles induced by said magnetic field introduces transient openings into said cell facilitating entry of said agent of interest into said cells.

Abstract: The disclosure relates to nerve derived adult pluripotent stem cells characterized by expression of Oct4, Sox2, c-Myc, and Klf4, methods for obtaining them, and their use.

Abstract: The disclosure generally relates to compositions and methods for the extraction of nucleic acids from biological samples. In particular embodiments the extraction involves mixing dibromochloromethane, iodochloromethane or mixtures thereof with a biological sample that has been mixed with a phenol-based extraction solution. Centrifugation of the sample is not needed to achieve phase separation which occurs using the present methods in as little as one to two minutes with no phenol carryover.

Abstract: Various aspects of the present disclosure are directed toward methods and apparatuses for interacting a first liquid and a second liquid in one or more fluidic channels of a capillary structure. The methods and apparatuses can include providing at least one capillary barrier that positions a meniscus of the first liquid at a fluid-interface region using capillary forces within the capillary structure. Additionally, a path is provided along one of the channels for the second liquid to flow toward the fluid-interface region. Additionally, gas pressure is released, via a gas-outflow port, from the fluid-interface region while flow of the first liquid is arrested. Further, the first liquid and the second liquid contact in the fluid-interface region with the capillary barrier holding the first liquid at the fluid-interface region.

Abstract: A novel method for preparing sequence-verified oligonucleotides is disclosed. In particular, the invention relates to a simple, affordable, and scalable method that combines high-throughput mating of yeast clones, a unique selectable system for combining DNA sequences in yeast, and next-generation sequencing. This method allows sequence-verified oligonucleotides to be readily isolated from complex libraries.

Abstract: Provided herein are methods and compositions to make guide nucleic acids (gNAs), nucleic acids encoding gNAs, collections of gNAs, and nucleic acids encoding for a collection of gNAs from any source nucleic acid. Also provided herein are methods and compositions to use the resulting gNAs, nucleic acids encoding gNAs, collections of gNAs, and nucleic acids encoding for a collection of gNAs in a variety of applications.