Abstract: Provided herein are conjugated immunoglobulins and methods for generating conjugated immunoglobulins using a microbial transglutaminase.

Abstract: A method for preparation of low molecular weight porcine lympho-reticular polypeptides. The method comprises the enzymatic hydrolysis of a source of protein, wherein the source of protein comprises a blend of porcine liver and porcine spleen, with an enzyme having proteolytic activity and an enzyme having amylase activity.

Abstract: The present invention is directed to methods of probiotic selection and use based on the capability of microbial biogenic amine uptake as a method for targeted clinical and veterinary applications, including promoting health and well-being and/or treating therapeutic conditions. The present invention utilizes a microbially-focused approach for the development of drug selection and/or probiotic administration in a variety of diseases and disorders.

Abstract: The majority of clinically used antibiotics and anticancer agents are derived from bacterial small molecules. These molecules are produced by dedicated biosynthetic gene clusters, sets of genes that are responsible for the step-wise generation of the target small molecule. Recent investigations have indicated, to the surprise of many experts, that the majority of these biosynthetic genes are inactive or ‘silent’ for unknown reasons. Thus under typical bacterial culturing conditions, these genes are not expressed and consequently the bioactive small molecule products are not synthesized. Disclosed is a method for high throughput screening of elicitors of cryptic metabolites, a method for producing cryptic metabolites, and a new family of cryptic metabolites, the acybolins, as well as their complete structural elucidation.

Abstract: Provided herein are compounds, enzyme substrates, compositions, kits, uses, and methods for detecting the presence or absence of a caspase enzyme, measuring the activity of a caspase enzyme, or detecting the presence or absence of apoptosis. The detection or measurement can occur through intracellular cleavage of a compound or enzyme substrate, which can lead to an increase in fluorescence, e.g., in the violet or red channel, through liberation of a nucleic acid binding dye from a peptide, such as liberation of a DNA-binding dye from a negatively charged peptide comprising a sequence recognized and cleaved by a caspase.

Abstract: Methods for the high-throughput analysis of transgenic events are herein disclosed. The methods use libraries of sheared genomic DNA ligated to specialized adapters and pooled for sequence analysis and comparison to known genomic and insert sequence. The method finds use in detecting characterizing insertion site, transgene integrity, and transgene copy number.

Abstract: A molecular manipulation system for investigating molecules, having a sample holder constructed to hold a sample comprising a plurality of molecules attached on one side to a surface in the sample holder and on another side attached to a microbead of a plurality of microbeads. The system having; an acoustic wave generator to generate an acoustic wave exerting a force on the microbeads in the sample; and a detector device to detect a response of the plurality of microbeads in the sample on the force exerted by the acoustic wave to investigate the molecules attached to the microbeads.

Abstract: The present disclosure provides methods and kits for highly multiplex single primer extensions using a MutS protein and Mg2+ at a concentration higher than that in a typical PCR reaction. Also disclosed is the use of such methods and kits in next generation sequencing.

Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.

Abstract: A method for real-time quantitative detection of single-type, target nucleic acid sequences amplified using a PCR in a microwell, comprising introducing in the microwell a sample comprising target nucleic acid sequences, magnetic primers, and labelling probes; performing an amplification cycle to form labelled amplicons; attracting the magnetic primers to a surface through a magnetic field to form a layer including labelled amplification products and free magnetic primers; and detecting the labelled amplification products in the layer with a surface-specific reading method.

Abstract: A receptacle transport mechanism comprises a body, a plunger configured to be movable with respect to the body, and one or more limbs movably attached to the body and positioned in operative engagement with the plunger. The plunger is movable with respect to the body between a first position whereby engagement by the plunger with the one or more limbs positions a lower portion of each limb proximal to the body and a second position whereby engagement by the plunger with the one or more limbs extends the lower portion of each limb outwardly relative to the body.

Abstract: The invention provides methods for determining aneuploidy and/or fetal fraction in maternal samples comprising fetal and maternal cfDNA by massively parallel sequencing. The method comprises a novel protocol for preparing sequencing libraries that unexpectedly improves the quality of library DNA while expediting the process of analysis of samples for prenatal diagnoses.

Abstract: Real time redox sequencing methods, devices, and systems are described. Arrays of redox devices comprising one or two electrodes are used to provide sequence information about a template nucleic acid in a polymerase-template complex bound proximate to the electrode(s). A sequencing reaction mixture comprising nucleotide analogs comprising redox labels is introduced to the array of redox devices under conditions of polymerase mediated nucleic acid synthesis. The time sequence of incorporation of nucleotide analogs is determined by electrochemically identifying the redox labels of the nucleotide analogs that are incorporated into the growing strand.

Abstract: The present invention relates functional ligands to target molecules, particularly to functional nucleic acids and modifications thereof, and to methods for simultaneously generating, for example, numerous different functional biomolecules, particularly to methods for generating numerous different functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to functional ligands which bind with affinity to target molecules, such as lipoarabinomannan (LAM).

Abstract: The present invention relates to methods of target nucleic acid amplification and uses thereof in methods of sequencing, or other down-stream applications such a genotyping or cloning. More specifically, the methods relate to methods of target nucleic acid amplification through the use of a universal hairpin primer.

Abstract: A method for identifying a renal allograft recipient at risk for chronic allograft damage or interstutial fibrosis and tubular atrophy (IF/TA) by comparing the transcription level of a preselected gene signature set with the transcription level of a comparison standard, and diagnosing the recipient as being at risk for chronic allograft damage if the transcription level of the preselected gene signature set is significantly higher than the transcription level of the comparison standard.

Abstract: This invention relates to a diagnostic test measuring circulating SCN5A proteins or gene transcripts in a test sample as a biomarker for pulmonary hypertension.

Abstract: This document provides methods and materials related to treating a disease. For example, this document provides methods for treating a subject's disease based on identifying the risk of progressive multifocal leukoencephalopathy PML using a genetic test.

Abstract: The present invention relates generally to a method of determining one or more probabilities of respective classifications of a neoplasm into one or more neoplastic categories. More particularly, the present invention relates to a method of determining the probability of classification of a large intestine neoplasm into one or more categories selected from adenoma, stage I, stage II, stage III or stage IV by screening for changes to the methylation levels of a panel of gene markers, including BCAT1, IKZF1, IRF4, GRASP and/or CAHM. The method of the present invention is useful in a range of applications including, but not limited to, those relating to the diagnosis and/or monitoring of colorectal neoplasms, such as colorectal adenocarcinosis.

Abstract: The invention provides methods for detecting a cellular proliferative disorder (e.g., urothelial cancer) in a subject by assessing the methylation status of the GULP1 promoter in a nucleic acid sample. The methods of the invention are useful for diagnostic, prognostic as well as therapeutic regimen predictions.

Abstract: The present invention relates to panels of methylation and mi RNA marker as well as their use in the prognosing, diagnosing and/or treatment of cancer, means for detecting said marker, kits comprising said means, and devices for analysing the marker panel.

Abstract: A method useful for identifying and isolating live circulating tumor cells is described. The method utilizes an adenoviral vector comprising a replication-competent adenovirus in which the E1 gene region is expressed under the control of a telomerase-specific promoter and further comprises a second expression cassette containing a marker protein, optionally fused to a detectable cell surface marker to permit detection of circulating tumor cells lacking cell surface markers. The method involves combining ex vivo a test sample from a patient suspected of having circulating tumor cells, an adenoviral probe system, and culture media for the cells. The test sample is incubated with the adenoviral system for a sufficient time to permit expression of the reporter protein. The marker gene expression can thereafter be quantitated and the marker-expressing cells may optionally be collected for further analysis.

Abstract: Laboratory-based high throughput methods and compositions (e.g., kits) for the detection of pathogen mRNA, including in particular, for the detection of SARS-CoV-2 RNA from up to 85,000 samples in one Illumina NovaSeq sequencing run. These methods may include barcoding cDNA, indexing pools of libraries and intensive use of automatic liquid handling, providing a ready-to-sequence library mix. These methods and compositions may allow a very inexpensive per sample cost and an assay that may be performed in about one day or less.

Abstract: In alternative embodiments, provided are non-natural or genetically engineered vinylisomerase-dehydratase enzymes, including alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases. In alternative embodiments, provided are non-natural or genetically engineered polypeptides having an activity comprising, for example, a vinylisomerase-dehydratase, an alkenol dehydratase, a linalool dehydratase and/or a crotyl alcohol dehydratase activity, or a combination thereof. In alternative embodiments, also provided are non-natural or genetically engineered nucleic acids (polynucleotides) encoding polypeptides described herein, expression or cloning vehicles comprising or having contained therein nucleic acids as described herein, and non-natural or genetically engineered cells comprising or having contained therein nucleic acids as described herein. In alternative embodiments, also provided are methods for making various organic compounds, including methyl vinyl carbinol and butadiene.

Abstract: A sandwich structure forming method including the steps of (1) providing a sandwich structure comprising a core positioned between a first liner sheet and a second liner sheet; (2) positioning the sandwich structure into a cavity of a die assembly; and (3) pressurizing the core to expand the sandwich structure into engagement with the die assembly.

Abstract: A steel sheet for a can having high strength, excellent ductility, and good corrosion resistance, and a method for manufacturing the steel sheet. The steel sheet has a chemical composition containing, by mass %, C: 0.020% or more and 0.130% or less, Si: 0.04% or less, Mn: 0.10% or more and 1.20% or less, P: 0.007% or more and 0.100% or less, S: 0.030% or less, Al: 0.001% or more and 0.100% or less, N: more than 0.0120% and 0.0200% or less, Nb: 0.0060% or more and 0.0300% or less, and Fe and inevitable impurities. An absolute value of a difference in an amount of solid solution Nb between a region from a surface to a position located at ? of a thickness and a region from a position located at ? of the thickness to a position located at 4/8 of the thickness is 0.0010 mass % or more.

Abstract: A method for manufacturing a non-oriented electrical steel sheet according to an exemplary embodiment of the present invention includes performing hot rolling on a slab after heating the slab to manufacture a hot rolled sheet; performing hot rolled sheet annealing on the hot rolled sheet; performing cold rolling on a steel sheet on which the hot rolled sheet annealing is completed to manufacture a cold rolled sheet; and performing cold rolled sheet annealing on the cold rolled sheet in which a difference between a cold rolled sheet annealing temperature in the cold rolled sheet annealing and a hot rolled sheet annealing temperature in the hot rolled sheet annealing is 100° C. or lower.

Abstract: In the production of a non-oriented electrical stress sheet by hot rolling a slab having a chemical composition comprising, by mass %, C: not more than 0.005, Si: 1.5-6.0, Mn: 0.05-2.0 and P: 0.03-0.15, subjecting to a hot band annealing, if necessary, cold rolling, finish annealing, and forming an insulation coating, the cooling from 700° C. to 500° C. in the finish annealing is conducted in an oxidizing atmosphere with an oxygen potential PH2O/PH2 of not less than 0.001 for 1-300 seconds, whereby P is segregated into the surface of the steel sheet after the finish annealing to obtain a non-oriented electrical steel sheet enhancing a crystal grain growth properties in the stress relief annealing.

Abstract: An aluminium-zinc-hot-dipped and colour-coated steel plate having a 600 MPa yield strength grade and a high elongation and a manufacturing method thereof, with the chemical components in mass percentage of a substrate of the steel plate being: 0.07-0.15% of C, 0.02-0.5% of Si, 1.3-1.8% of Mn, N?0.004%, S?0.01%, Ti?0.20%, Nb?0.060%, and the balance being Fe and other inevitable impurities, and meanwhile satisfying the conditions of: (C+Mn/6)?0.3%; Mn/S?150; Nb satisfying 0.01%?(Nb?0.22C?1.1N)?0.06% where no Ti is contained; Ti satisfying 0.5?Ti/C?1.5 where no Nb is contained; and 0.04%?(Ti+Nb)?0.26% where Ti and Nb are added in combination. The steel plate has a yield strength of ?600 MPa, a tensile strength of ?650 MPa, an elongation after fracture of ?12%, a good strength and toughness and an excellent corrosion resistance.

Abstract: A method for producing a coated steel sheet by reheating a steel slab containing 0.15-0.25 wt % of carbon (C), more than 0 wt % but not more than 1.5 wt % of silicon (Si), 1.5-2.5 wt % of manganese (Mn), more than 0 wt % but not more than 1.8 wt % of aluminum (Al), 0.3-1.0 wt % of chromium (Cr), more than 0 wt % but not more than 0.03 wt % of titanium (Ti), more than 0 wt % but not more than 0.03 wt % of niobium (Nb), and the balance of iron (Fe) and unavoidable impurities. Hot-rolling, cooling and coiling the steel slab, thereby producing a hot-rolled steel sheet. Pickling the hot-rolled steel sheet, then cold rolling. Annealing the cold-rolled steel sheet at a temperature between 820° C. and 870° C., followed by cooling at a finish-cooling temperature between 350° C. and 450° C.; tempering the cooled steel sheet at a temperature between 450° C. and 550° C.; and hot-dip galvanizing the tempered steel sheet.

Abstract: A steel sheet is provided, having a tensile strength of 340 MPa or more, a particular composition and a steel structure in which ferrite has an average crystal grain size of 5 ?m or more and 25 ?m or less, 40% or more of cementite in terms of area fraction precipitates in ferrite grain boundaries, and the ferrite has a texture in which an inverse intensity ratio of ?-fiber to ?-fiber is 0.8 or more and 7.0 or less.

Abstract: An on-line intelligent control method for the cooling characteristics of a quenching liquid in heat treatment production includes the steps of: step 1: subjecting a workpiece to thermal insulation; step 2: measuring the cooling characteristics and the heat transfer coefficient of a quenching liquid followed by correction; step 3: starting cooling; step 4: then changing the internal circulation rate; and step 5: removing the workpiece. This scheme can effectively avoid the problem that the cooling of a workpiece in industrial production deviates from the ideal cooling characteristics of a quenching liquid obtained in a laboratory.

Abstract: The present invention relates to a HEA foam prepared by selective dissolution of a second phase within a two-phase separating alloy comprising the HEA and a manufacturing method thereof. The manufacturing method of the HEA foam of the present invention has the effect of preparing a novel HEA foam, which was not available in the past, by leaving only a first phase after manufacturing a two-phase separating alloy comprising a first phase by HEA, wherein at least 3 metal elements act as a common solvent. Furthermore, the HEA foam of the present invention has a structure, wherein pores are distributed inside the HEA, in which at least 3 metal elements act as a common solvent. By adding a functional characteristic of low heat conductivity, etc., to the existing high strength characteristic of HEA, the HEA foam of the present invention can exhibit a complex effect by the combination of the two particular effects, thereby being capable of exhibiting excellent physical characteristics.

Abstract: Metal-nanoparticle composites, such as metal-boron nitride nanoparticle composites, and methods of manufacturing the same are provided. Ultrasonic casting techniques can be used to achieve uniform dispersion of nanoparticles, such as boron nitride nanotubes (BNNTs) in a metal matrix, such as aluminum. The BNNTs can be incorporated into the melt of the metal, and ultrasonic treatment can then be applied.

Abstract: The Cu-based sintered sliding material has a composition including, by mass %, 7% to 35% of Ni, 1% to 10% of Sn, 0.9% to 3% of P, and 0.5% to 5% of C, with a remainder of Cu and inevitable impurities, wherein the Cu-based sintered sliding material includes a sintered body including: alloy grains that contain Sn and C and contain a Cu—Ni-based alloy as a main component; grain boundary phases that contain Ni and P as main components and are dispersedly distributed in grain boundaries of the alloy grains; and free graphite that intervenes at the grain boundaries of the alloy grains, the Cu-based sintered sliding material has a structure in which pores are dispersedly formed in the grain boundaries of the alloy grains, and an amount of C in a metal matrix including the alloy grains and the grain boundary phases is, by mass %, 0.02% to 0.20%.

Abstract: A Ni-base superalloy composition to be used for powder-based additive manufacturing (AM) technology, such as selective laser melting (SLM) or electron beam melting (EBM). The cracking susceptibility during an AM process is considerably reduced by controlling the amount of elements, especially Hf, that form low-melting eutectics.

Abstract: Provided is a cold-rolled steel sheet, a galvanized steel sheet and a galvannealed steel sheet, comprising, by wt %, 0.06-0.15% of C, 1.2% or less (excluding 0%) of Si, 1.7-2.7% of Mn, 0.15% or less (excluding 0%) of Mo, 1.0% or less (excluding 0%) of Cr, 0.1% or less (excluding 0%) of P, 0.01% or less (excluding 0%) of S, 0.001-0.04% of Ti, 0.001-0.04% of Nb, 0.01% or less (excluding 0%) of N, 0.01% or less (excluding 0%) of B, and a remainder of Fe and unavoidable impurities, wherein the Si, C, Mn, Mo and Cr contents satisfy Relationship 1, and wherein a microstructure comprising, in area %, 10-70% of ferrite, 10-50% of the sum of bainite and retained austenite, and a remainder of fresh martensite, wherein a ratio (Mb/Mt) is 60% or more, and methods of manufacturing these steel sheets. ([Si]+[C]×3)/([Mn]+[Mo]+[Cr])?0.18.

Abstract: Provided is a high tensile strength steel having a tensile strength of 780 MPa grade or higher which is used for structural members of automobiles, and more specifically relates to high tensile strength steel having excellent bendability and stretch-flangeability while still satisfying characteristics of DP steels of low yield ratio and high ductility, and to a manufacturing method thereof.

Abstract: The present disclosure relates to a martensitic stainless steel suitable for rock drill steel rods. Furthermore, the present disclosure also relates to the use of the martensitic stainless steel and to products manufactured thereof, especially drill rods.

Abstract: A Cr—Mn—N austenitic heat-resistant steel is provided. The heat-resistant steel comprises, in weight percentage, carbon 0.20% to 0.50%, silicon 0.50% to 2.00%, manganese 2.00% to 5.00%, phosphorus less than 0.04%, sulphur less than 0.03%, chromium 20.00% to 27.00%, nickel 6.00% to 8.00%, molybdenum less than 0.50%, niobium less than 0.60%, tungsten less than 0.60%, vanadium less than 0.15%, nitrogen 0.30% to 0.60%, zirconium less than 0.10%, cobalt less than 0.10%, yttrium less than 0.10%, boron less than 0.20%, with the balance iron. The heat-resistant steel has high temperature strength, high thermal conductivity, low thermal expansion coefficient, good dimensional stability, good ductility, heat resistance, impact resistance, and low production costs, and meets the requirements for high performance engines.

Abstract: Provided are a high-strength steel sheet having a specified chemical composition, in which a Mn-segregation degree in a region within 100 ?m from a surface thereof in a thickness direction is 1.5 or less, in a plane parallel to the surface of the steel sheet in a region within 100 ?m from the surface of the steel sheet in the thickness direction, the number of oxide-based inclusion grains having a grain long diameter of 5 ?m or more is 1000 or less/100 mm2, a proportion of the number of oxide-based inclusion grains having a chemical composition containing alumina of 50 mass % or more, silica of 20 mass % or less, and calcia of 40 mass % or less to the total number of oxide-based inclusions having a grain long diameter of 5 ?m or more is 80% or more, a specified metallographic structure, and a TS of 980 MPa or more, a high-strength galvanized steel sheet, and a manufacturing method thereof.

Abstract: Systems and methods for drawing high aspect ratio metallic glass-based materials are provided. Methods of drawing a high aspect ratio metallic glass-based material are premised on stably drawing high aspect ratio metallic glass-based material from a preform metallic glass-based composition, accounting for the relationships between: the desired formation of an amorphous structure that is substantially homogenous along the majority of the length of the drawn high aspect ratio material; the desired final geometry of the drawn high aspect ratio material; the nature of the force that is used to draw the molten metallic glass-based composition; the velocity at which the high aspect ratio material is drawn; the viscosity profile of the material along its length as it is being drawn; and/or the effect of temperature on the metallic glass-based material.

Abstract: Aluminum alloys are provided. The alloys can include one or more of zinc, magnesium, copper, zirconium, yttrium, erbium, ytterbium, scandium, silver, and the balance of aluminum and incidental elements and impurities. The alloys can be used for additive manufacturing of various articles, such as aircraft components.

Abstract: An apparatus for the continuous hot dip coating of a metal strip is provided The apparatus includes a vessel intended to contain a liquid metal bath, a bottom roller and a scroll casing of the metal strip. The casing includes, at its lower end, a pouring box delimiting a front pouring compartment for liquid metal and a rear pouring compartment for liquid metal. Each pouring compartment is inwardly delimited by an inner wall and outwardly delimited by an outer wall. The outer wall of the rear pouring compartment forms, with the passage plane of the metal strip, an angle greater than or equal to 15° in the usage configuration. A method is also provided.

Abstract: A method for producing a high-strength galvanized steel sheet having excellent fatigue resistance properties. The method includes an oxidation processing in which a steel sheet is heated at a temperature of 400 to 750° C. in an atmosphere having an O2 concentration of 1000 ppm by volume or more and a H2O concentration of 1000 ppm by volume or more, and the steel sheet is heated at a temperature of 600 to 850° C. in an atmosphere having an O2 concentration of less than 1000 ppm by volume and a H2O concentration of 1000 ppm by volume or more. The method also includes reduction-annealing in which the steel sheet is heated at a heating rate of 0.1° C./sec or more to a temperature of 650 to 900° C. in an atmosphere having a H2 concentration of 5 to 30 vol % and a H2O concentration of 10 to 1000 ppm by volume.

Abstract: High strength steel sheets that have excellent ductility and low-temperature toughness and methods for producing such high strength steel sheets. A high strength steel sheet includes a composition containing, on a mass % basis, C: 0.05% to 0.30%, Si: 0.5% to 2.5%, Mn: 0.5% to 3.5%, P: 0.003% to 0.100%, S: 0.02% or less, Al: 0.010% to 1.5%, and N: 0.01% or less, the balance being Fe and unavoidable impurities, and a steel microstructure including a ferrite phase with an area fraction of 10% to 70%, a hard second phase with an area fraction of 30% to 90%, and a carbide that is at an interface between a ferrite phase and a hard second phase and that has an average equivalent-circle diameter of 200 nm or less.

Abstract: A susceptor for receiving a substrate, the susceptor including a ceramics base member, a conductive layer formed on a top surface of the ceramics base member and a side surface of the ceramics base member, the top surface of the ceramics base member configured to receive and support the substrate, and a conducting member contacting the conductive layer at an outer surface of the ceramics base member.

Abstract: An arc wire spraying method includes conveying at least two wires out of respective lance nozzles of a wire conveying device by means of the wire conveying device, applying current to the two wires to form an arc for melting the ends of the two wires, and applying airflow to the arc in the direction transverse to the longitudinal direction of the wire conveying device by means of an airflow applying device so as to spray the melted wire material toward a surface to be sprayed. The airflow applying device rotationally applies the airflow around the longitudinal direction of the wire conveying device. Parameters for spraying are variably adjusted along the rotating direction of the airflow applying device. The airflow is rotationally applied at a varying rotating speed.

Abstract: An ion source enhanced AlCrSiN coating for a cutting tool is provided. The ion source enhanced AlCrSiN coaling has gradient Si content and grain size, including sequentially an AlCrSiN working layer, an interlayer and an AlCrN bottom layer in order from a surface of the coating to a substrate, wherein from the AlCrN bottom layer to the AlCrSiN working layer, Si content in the interlayer is gradually increased, and the interlayer has a texture that changes from coarse columnar crystals to fine nanocrystals and amorphous body. A texture of the coating, in which the grain size is gradually decreased, sequentially includes coarse columnar crystals, fine columnar crystals and fine equiaxed crystals. A method for preparing the ion source enhanced AlCrSiN coating with the gradient Si content and grain size is provided as well as a cutting tool having the coating deposited thereon.

Abstract: A method to transfer a layer of harder thin film substrate onto a softer, flexible substrate. In particular, the present invention provides a method to deposit a layer of sapphire thin film on to a softer and flexible substrate e.g. PET, polymers, plastics, paper and fabrics. This combination provides the hardness of sapphire thin film to softer flexible substrates.