Abstract: The present disclosure relates in some aspects to methods and compositions for analysis of a target nucleic acid, such as in situ detection of a region of interest in a polynucleotide in a tissue sample. In some aspects, provided herein are methods and compositions for detecting a region of interest in a target nucleic acid. In some aspects, provided herein are circularizable probes and oligonucleotide probes for analyzing a target nucleic acid, in which corresponding hybridization regions between the circularizable probe and the oligonucleotide probe comprise a barcode region comprising one or more barcodes. In some aspects, the oligonucleotide probe and/or the circularizable probe comprise one or more modifications.

Abstract: The present disclosure provides compositions, methods, and kits comprising a nano-partition contained within a partition that allows for the separation and use of at least two enzymes in a partition-based assay of a biological sample, such as a single cell in a droplet. The compositions and methods are useful for carrying out partition-based assays of fixed biological samples.

Abstract: Embodiments of the instant disclosure relate to novel compositions and methods for analyte detection by quantifying transcription of RNA using CRISPR-based approaches. In certain embodiments, constructs of use in assays disclosed herein can include a novel double-stranded DNA sequence having at least one transcriptional promoter, at least one regulatory element, and a target sequence. In some embodiments, constructs disclosed herein can be used in a system for quantifying transcriptional output in a sample in order to detect presence of an analyte. In other embodiments, constructs and systems disclosed herein can be used to measure enzyme activity, to detect and/or quantify at least one agent that modulates transcription, detect and/or quantify analytes (e.g. a contaminant, biomarkers or other agent) or a combination thereof in accordance with assessing health of a subject or assessing environmental conditions or contaminants.

Abstract: The present disclosure provides compositions comprising nucleic acid double-stranded splint adaptors, including kits, and methods that employ the double-stranded splint adaptors. The double-stranded splint adaptors (200) can be used in a one-pot, multi-enzyme reaction to introduce one or more new adaptor sequences into a library molecule. The double-stranded splint adaptor (200) comprises a first splint strand (long splint strand (300)) and a second splint strand (short splint strand (400)), where the first and second splint strands are hybridized together to form the double-stranded splint adaptor (200) having a double-stranded region and two flanking single-stranded regions. The second splint strand (400) carries the new adaptor sequence(s) to be introduced, such as for example a universal binding sequence and/or an index sequence.

Abstract: Methods of volumetric imaging of a three-dimensional matrix of nucleic acids within a cell is provided. An automated apparatus for sequencing and volumetric imaging of a three-dimensional matrix of nucleic acids is provided.

Abstract: The disclosure provides a novel system of storing information using a charged polymer, e.g., DNA, the monomers of which correspond to a machine-readable code, e.g., a binary code, and which can be synthesized and/or read using a novel nanochip device comprising nanopores; novel methods and devices for synthesizing oligonucleotides in a nanochip format; novel methods for synthesizing DNA in the 3? to 5? direction using topoisomerase; novel methods and devices for reading the sequence of a charged polymer, e.g., DNA, by measuring capacitive or impedance variance, e.g., via a change in a resonant frequency response, as the polymer passes through the nanopore; and further provides compounds, compositions, methods and devices useful therein.

Abstract: The present disclosure provides methods and systems for sequencing nucleic acid molecules using a single frequency during detection, or fewer frequencies than types of nucleotide bases identified during detection. Methods and systems of the present disclosure may involve transiently binding nucleotides. Methods and systems provided herein may enable sequences to be determined at a higher accuracy and efficiency.

Abstract: Provided herein methods, systems, and apparatus for high throughput sequencing, such as at an industrial scale. A sequencing system and/or apparatus may comprise one or more stations that can be operated in parallel and/or independent of one or more other stations.

Abstract: A method of using a sequencing cell includes applying voltage across the sequencing cell, acquiring one or more signal values from the sequencing cell, and acquiring one or more correlated signal values that are correlated with respective values of the plurality of acquired signal values thereby forming a plurality of two-dimensional data points. The plurality of two-dimensional data points comprise values in a first dimension that equal the plurality of acquired signal value and values in a second dimension that equal the plurality of correlated signal values. The method can further include computing a plurality of transformed signal values by applying a two-dimensional transformation to the plurality of two-dimensional data points.

Abstract: Aspects of the present disclosure include methods of making barcoded solid supports. In some embodiments, the methods include producing a concatemer by rolling circle amplification (RCA) of a circular nucleic acid template, where the circular nucleic acid template includes a barcode and a stem-loop forming region, and where the concatemer includes a plurality of linked units, each unit including the barcode and a stem-loop structure formed from the stem-loop forming region. Such methods further include disposing the concatemer on a solid support to produce a barcoded solid support including a plurality of the stem-loop structures extending from the surface of the solid support. The methods may further include treating the stem-loop structures with an agent that produces stem structures having ends compatible with target nucleic acids, and attaching the target nucleic acids to the stem structures. Barcoded solid supports and methods of using the barcoded solid supports are also provided.

Abstract: The invention provides sets of RNA depletion probes, short DNA oligos that hybridize along the length of a target RNA and mediate digestion of the target RNA by RNase H to remove super-abundant RNA molecules from a sample. Depletion probes according to the invention are designed foremost based on biochemistry and the biophysical properties of the probes so that all of the depletion probes of a set exhibit substantially uniform, consistent behavior in binding to a target RNA in a sample. Probes are principally designed to specific performance targets and biophysical properties, yielding probe sets with irregular, even apparently random, spacing along a target RNA molecule.

Abstract: Described herein are methods and systems for identifying subpopulations of patients having Crohn's disease, including populations at risk of developing stricturing or other severe disease, and populations susceptible to success or failure with surgical intervention. Further provided are therapies useful for treating subpopulations of patients having Crohn's disease.

Abstract: The invention relates to an in vitro or ex vivo method for determining the risk of incidence of a care-related infection in a patient, including a step of measuring the expression of CIITA in a biological sample from said patient.

Abstract: The present disclosure provides kits, methods, and assays for detecting one or more mutations associated with hereditary or syndromic hearing loss. The method can comprise performing a multigene panel sequencing assay on a biological sample extracted from a subject to identify one or more mutations associated with hearing loss. The disclosed kits, methods, and assays may be particularly useful for determining whether an infant patient is a carrier for or is at risk for developing a hereditary hearing loss-related disorder such as Usher syndrome, Pendred syndrome, Jervell syndrome, and Lange-Nielsen syndrome.

Abstract: Provided herein are methods, processes and apparatuses for non-invasive assessment of genetic variations.

Abstract: The present invention relates to molecular signatures in genomic DNA that are associated with sensitivity of tumors, such as HPV-associated tumors, to treatment with radiation therapy. The present invention provides methods of analyzing tissue samples, or DNA obtained therefrom, to assess the presence of such molecular signatures, methods of predicting whether a tumor in a subject will be sensitive to treatment with radiation therapy based on the presence of such signatures, and methods of treating subjects with radiation therapy if such signatures are present.

Abstract: Disclosed herein is a clear cell renal cell carcinoma (ccRCC) biomarker set. Also disclosed herein is a detection system using the biomarker set disclosed herein, methods of determining whether a subject has or shows recurrence of clear cell renal cell carcinoma, method of determining whether a renal mass sample is benign or malignant, method of detecting response of a subject to systemic treatment, and a kit for carrying out the same.

Abstract: The present invention discloses a combination of DNA methylation markers and the use thereof, primers, probes and a kit for early detection of ovarian cancer, wherein the combination of DNA methylation markers comprises at least one methylated fragment of each of four genes, including PCDHB18P, CDO1, HOXA9 and LYPD5. The detection primers and kit obtained for the methylated regions of the target genes can significantly improve the sensitivity of ovarian cancer detection, and meanwhile maintain a high specificity for normal ovarian cells and benign ovarian tumors. The detection primers and probes detect patients with possible gynecological malignant tumors early through molecular epigenetic methods using methylation detection technology, and the result is very accurate, and can provide clinicians with auxiliary diagnostic reference for early prophylactic treatment.

Abstract: Methods and systems described herein include using various characteristics of cell-free DNA molecules to determine a property of a biological sample or a subject. Such characteristics can include size (e.g., where characteristic is of long cell-free DNA molecules), methylation, and end motifs. The method includes determining disease classification and/or predicting tissue of origin. In some instances, the characteristics includes determining an amount of long cell-free DNA molecules, and the disease classification can be based on the determined amount. The characteristics can also include identifying methylation pattern of a cell-free DNA molecule, then comparing the methylation pattern to a reference pattern to predict the tissue origin. In some instances, the methylation-pattern analysis includes using a trained machine-learning model.

Abstract: Disclosed are a genetic tumor marker combination, a methylation detection reagent, a kit, and use thereof. Lung cancer specimens can be distinguished by measuring the methylation level of a combination of HOXB4 and SRCIN1 genes. Proved by experiments, the reagent of the present disclosure can detect and diagnose lung cancer, and has clinical application value.

Abstract: The present invention refers to BMP8B-inhibitor for use in a method of preventing and/or treating liver fibrosis and/or liver cancer, wherein the inhibitor is an oligonucleotide, an antibody or fragment thereof, a small molecule or a combination thereof. Further, the invention is directed to a method for diagnosing liver fibrosis and/or liver cancer and a kit for diagnosing fibrosis and/or cancer. The method and the kit can be used to identify a subject in need for a BMP8B-inhibitor to prevent and/or treat liver fibrosis and/or liver cancer.

Abstract: Disclosed herein are methods of treating and identifying subjects with ER+ breast cancer that will or will not develop resistance of tamoxifen. In some examples such methods include measuring expression of adaptor-related protein complex 2, sigma-1 subunit (AP2S1) from the retrograde neurotrophin signaling pathway, cyclin-dependent kinase 2 (CDC2) from the loss of NLP from mitotic centrosomes pathway, general transcription factor IIIC subunit 3 (GTF3C3) from the RNA polymerase III transcription initiation from Type 2 promoter pathway, eukaryotic translation initiation factor 2-alpha kinase 3 (EIFA2AK3) from the EIF2 pathway, and leucyl-tRNA synthetase (LARS) from the valine, leucine and isoleucine biosynthesis pathway.

Abstract: Diagnostic methods for identifying cancer bearing subjects appropriate for treatment with CPX-351 include genetic and ex vivo testing of cells from a candidate subject. Combination treatment with CPX-351 and FLT-3 inhibitors improve CPX-351 uptake and toxicity.

Abstract: The invention discloses a fluorescent quantitative PCR technology-based method for distinguishing human DNA, and a fluorescent quantitative PCR technology-based composition or kit for distinguishing human DNA, comprising primers and/or probes of nucleotide sequences.

Abstract: In one aspect, the invention provides methods for determining the contributions of canid populations to a canid genome. The methods comprise the steps of: (a) obtaining the identity of one or both alleles in a test canid genome for each of a set of markers; and (b) determining the contributions of canid populations to the test canid genome by comparing the alleles in the test canid genome to a database comprising canid population profiles, wherein each canid population profile comprises genotype information for the set of markers in the canid populations.

Abstract: In an embodiment, the present disclosure pertains to a method of detecting an analyte from vesicles in a sample. In an additional embodiment, the present disclosure pertains to an analyte detection platform. In a further embodiment, the present disclosure pertains to a sensor. In another embodiment, the present disclosure pertains to a method of detecting an analyte from a sample. In an additional embodiment, the present disclosure pertains to a method of lysing vesicles. In a further embodiment, the present disclosure pertains to a vesicle lysis platform.

Abstract: A method of testing milk samples for the presence of pathogens. Sample day milk may be processed using specific reagents for the detection of pathogens and then subjected to polymerase chain reaction (PCR) amplification using multi-well plates for fast and accurate detection of the presence of nucleotides from specific pathogens. The sample day milk can be collected non-aseptically without compromising the accuracy of the detection results.

Abstract: The invention provides a transgenic corn event ZM_BCS216090, plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising event ZM_BCS216090. The invention also provides polynucleotides and sequences specific for event ZM_BCS216090 and methods for using plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising event ZM_BCS216090 and detecting event ZM_BCS216090, or a polynucleotide or DNA sequence specific for event ZM_BCS216090, in a DNA molecule or sample.

Abstract: An apparatus for sampling and detecting a pathogen in air comprising a first air sampling module, a second module for the isolation of viral RNA and a third module for amplification and detection of the viral RNA; thanks to the integration between the modules the apparatus is compact and portable and can be used for in situ sampling of air in closed environments such as ambulances or hospital rooms, air in the external environment and air exhaled by patients.

Abstract: The invention relates to methods for quantifying a SARS coronavirus in a blood sample of a subject, and for determining a risk for the subject to develop a severe form of the disease or for the disease to worsen.

Abstract: The present invention provides methods for rapid and efficient isolation and characterization of nucleic acid, particularly RNA, from small volume and self-collected samples, particularly saliva samples, to determine the presence or absence of infectious agent RNA, particularly viral RNA, particularly infectious viral RNA, particularly coronavirus. The invention provides methods for isolation and evaluation of infectious agent RNA from saliva samples, particularly viral agents, particularly coronavirus. The invention further provides methods and strategies for pooling samples and RNA to determine the presence or absence of infectious agent RNA, particularly viral RNA, particularly infectious viral RNA, particularly coronavirus, with regard to large numbers of samples at one time and in a single pooled test format.

Abstract: Described herein are systems and methods for processing at least one biological sample. The systems and methods may process the biological sample, or plurality thereof, using at least one droplet. The droplet, or plurality thereof, may be manipulated using the systems and methods described herein.

Abstract: The present disclosure provides methods and compositions for determining whether a patient exhibiting acute gastroenteritis will benefit from treatment with therapeutic agents that inhibit Norovirus genogroup I (GI) or Norovirus genogroup II (GII). The methods disclosed herein are based on detecting Norovirus genogroup I (GI) and Norovirus genogroup II (GII) in a stool sample without extracting viral nucleic acids from a clinical specimen prior to performing real-time reverse transcription PCR. Kits for use in practicing the methods are also provided.

Abstract: The present invention provides a method for producing dried and stable reagent:substrate complexes suitable for use in various types of assays. Also provided are dried reagent:substrate complexes produced by the method of the invention, as well as methods for their use to perform an assay.

Abstract: Provided is a composition for manufacturing leather comprising: hydroxide and borax (Na2B4O7•10H2O).

Abstract: A burner comprises a primary nozzle for injecting an oxygen-rich gas. The primary nozzle is designed as a supersonic nozzle. A coaxial nozzle having an annular outlet opening is provided for injecting a fuel gas. The coaxial nozzle is designed as a subsonic nozzle and is coaxial to the primary nozzle. The primary nozzle has a convergent portion and a divergent portion, which adjoin each other at a radius of the narrowest cross-section. The annular outlet opening is located at an end face of the burner. The fuel gas, in the form of hydrogen or a mixture of hydrogen and a hydrocarbon-containing gas, is injected at a fixed inlet pressure and a fixed inlet volumetric flow rate, with respect to a planned thermal power of the burner. In contrast, the inlet pressure and the inlet volumetric flow rate of the oxygen-rich gas are varied according to the application.

Abstract: A rail has excellent fatigue crack propagation resistance characteristics, wherein the rail has a component composition including C: 0.80 to 1.30 mass %, Si: 0.10 to 1.20 mass %, Mn: 0.20 to 1.80 mass %, P: not more than 0.035 mass %, S: 0.0005 to 0.012 mass %, Cr: 0.20 to 2.50 mass % and the remainder being Fe and inevitable impurities and satisfying CP represented by equation (1) being not more than 2500: CP=X/RA??(1) and X={(10×[% C])+([% Si]/12)+([% Mn]/24)+([% Cr]/21)}5??(2), where [% Y] is content of an element Y (mass %), and RA is a prior austenite grain size (?m).

Abstract: The present invention relates to material utilized for heavy construction machinery, vehicle frames, reinforcing members, and the like, and more specifically to a high-strength steel sheet having excellent impact resistance and a method for manufacturing same.

Abstract: A lixiviant medium for extracting gold from a gold-containing solid material contains a polyether-modified siloxane and a non-ionic surfactant. The lixiviant medium can be used in a method of extracting gold from a gold-containing solid material. The lixiviant medium can be prepared from a composition containing a non-ionic surfactant and a polyether-modified siloxane. A method of preparing the lixiviant medium involves mixing the composition with an alkali metal cyanide, a pH regulator suitable for setting a pH of the composition to a value of 9 or higher, and water.

Abstract: A method of recovering lead, antimony tin from lead acid batteries, lead bearing scrap and other lead bearing materials which includes smelting lead bearing materials in a reverb furnace to recover metallic lead; leaching the resultant slag produced in the reverb furnace with ammonium chloride (NH4Cl) to produce a slurry; precipitating antimony from the slurry with ferric chloride (FeCl3); performing a solid-liquid separation of the slag away from the resulting pregnant leach solution; precipitating lead carbonate (PbCO3) from the pregnant leach solution with carbon dioxide (CO2); recovering the precipitated lead carbonate (PbCO3) through solid-liquid separation; and processing the precipitated lead carbonate (PbCO3) in a reverb furnace to recover metallic lead.

Abstract: Provided is a method for recovering lithium from a material containing fluorine and lithium. The method includes: mixing the material with an aqueous sulfuric acid solution or water to prepare a mixed liquid; and mixing the mixed liquid with a slightly or sparingly soluble calcium-containing alkaline agent to form a precipitate containing fluorine and calcium, followed by solid-liquid separation to obtain a purified solution having lithium dissolved therein. In the preparation of the a mixed liquid, the material and the sulfuric acid aqueous solution or water are mixed and heated at 60° to 90° C.

Abstract: A method for dissolving a lithium compound according to the present invention includes bringing a lithium compound into contact with water or an acidic solution, and feeding, separately from the lithium compound, a carbonate ion to the water or the acidic solution to produce carbonic acid, and allowing the carbonic acid to react with the lithium compound to produce lithium hydrogen carbonate.

Abstract: There are provided an inexpensive copper powder, which has a low content of oxygen even it has a small particle diameter and which has a high shrinkage starting temperature when it is heated, and a method for producing the same. While a molten metal of copper heated to a temperature, which is higher than the melting point of copper by 250 to 700° C. (preferably 350 to 650° C. and more preferably 450 to 600° C.), is allowed to drop, a high-pressure water is sprayed onto the heated molten metal of copper in a non-oxidizing atmosphere (such as an atmosphere of nitrogen, argon, hydrogen or carbon monoxide) to rapidly cool and solidify the heated molten metal of copper to produce a copper powder which has an average particle diameter of 1 to 10 ?m and a crystallite diameter Dx(200) of not less than 40 nm on (200) plane thereof, the content of oxygen in the copper powder being 0.7% by weight or less.

Abstract: A magnesium alloy can include magnesium, about 3.4 wt % to about 5.5 wt % aluminum, about 0.40 wt % to about 1.5 wt % zinc, and about 0.26 wt % to about 0.36 wt % manganese. The magnesium alloy may exhibit an ultimate tensile strength from about 210 MPa to about 260 MPa, a yield strength from about 100 MPa to about 135 MPa, and an elongation from about 8% to about 15%. The magnesium alloy may exhibit a bend angle from about 46° to about 54°.

Abstract: A sintered cemented carbide body including tungsten carbide, and a substantially cobalt-free binder including an iron-based alloy sintered with the tungsten carbide. The iron-based alloy is approximately 2-25% of the overall weight percentage of the sintered tungsten carbide and iron-based alloy. The tungsten carbide may be approximately 90 wt % and the iron-based alloy may be approximately 10 wt % of the overall weight percentage of the sintered tungsten carbide and iron-based alloy. The tungsten carbide may comprise a substantially same size before and after undergoing sintering. The iron-based alloy may be sintered with the tungsten carbide using a uniaxial hot pressing process, a spark plasma sintering process, or a pressureless sintering process. The sintered tungsten carbide and iron-based alloy has a hardness value of at least 15 GPa and a fracture toughness value of at least 11 MPa?m.

Abstract: An alloy composed of three or more types of elements, wherein all the standard deviation of distribution in the alloy of each element constituting the alloy are 15 atomic % or less provides a novel alloy composed of three or more types of elements and having a high solid solution uniformity.

Abstract: A method for producing steel strip, in particular hot strip in the form of coiled coils or in the form of folded individual sheets, in which a steel melt is first produced, this is then formed into a strand in a continuous casting system, the strand is then fed into a heating unit and the heated strand is then rolled into hot strip in a subsequent rolling mill. The casting of the strand, the passage through the heating unit, and the rolling take place in a continuous process. To be able to produce hot-rolled steel strips in the most energy-efficient way possible and to make these strips available for further processing into high-quality cold-rolled and, if necessary, coated strips, the invention provides that, first of all, a steel melt is produced.

Abstract: A cold rolled and annealed steel sheet, made of a steel having a composition including, by weight percent: C: 0.03-0.18%, Mn: 6.0-11.0%, Al: 0.2-3%, Mo: 0.05-0.5%, B: 0.0005-0.005%, S 0.010%, P 0.020%, N 0.008%, and including optionally one or more of the following elements, in weight percentage: Si?1.20%, Ti?0.050%, Nb?0.050%, Cr?0.5%, V?0.2%, the remainder of the composition being iron and unavoidable impurities resulting from the smelting, the steel sheet having a microstructure including, in surface fraction, from 25% to 55% of retained austenite, from 45% to 75% of ferrite, less than 5% of fresh martensite, a carbon [C]A and manganese [Mn]A content in austenite, expressed in weight percent, such that the ratio ([C]A×[Mn]2A)/(C %×Mn %) is from 19.0 to 41.0 wt %, C % and Mn % being the nominal values in carbon and manganese in weight % and a carbides density below 3×106/mm2 and—an inhomogeneous repartition of manganese characterized by a manganese distribution with a slope above or equal to ?30.

Abstract: The present invention belongs to the field of mechanical engineering and materials, more specifically in the metallurgy segments, for application in the sugar industry. The invention relates to the hot forging process of mill shafts and heat-treated auxiliary equipment in CrNiMo and low carbon alloy. The shafts and auxiliary equipment manufactured according to this invention have a long service life, thus reducing downtime for maintenance and increasing reliability, since it eliminates the problems associated with cracks and instantaneous fractures. As a consequence, there is a reduction in the risks of accidents and production losses associated with stoppages.

Abstract: A paramagnetic stainless steel with a chemical composition may include iron and, by weight: 20?Cr?40%; 3?Ni?20%; 0?Mn?15%; 0?Al?5%; 3?Mo?15%; 0?W?5%; 0?Cu?2%; 0?Si?5%; 0?Ti?1%; 0?Nb?1%; 0?C?0.1%; 0?N?0.5%; 0?S?0.5%; 0?P?0.1%, and optionally impurities, each at a concentration of less than or equal to 0.5%. The steel may have a hardness in a range of from 575 to 900 HV10. A part, in particular a timepiece component, may be made from or include this steel.