Abstract: Described herein are nucleic acid detection compositions and systems comprising an internal nuclease chain reaction (NCR) for signal amplification and methods of using these NCR-containing compositions and systems.

Abstract: The subject invention relates to a contamination-free method that utilizes CRISPR/Cas enzyme specific sequence recognition towards the loop region in the LAMP amplicons to accomplish one-pot detection for rapid visual readout diagnostics of the presence or absence of target nucleic acid sequences.

Abstract: Disclosed are compositions, systems, kits, and methods for detecting an analyte or target molecule in a sample by regulated in vitro transcription. The compositions, systems, kits, and methods typically comprise and/or utilize one or more components selected from: (a) an RNA polymerase; (b) an allosteric transcription factor (aTF), wherein the aTF binds an analyte or target molecule as a ligand; (c) an engineered transcription template; (d) a dsDNA signal gate molecule; and/or any combination thereof.

Abstract: In various embodiments methods of determining methylation of DNA are provided.

Abstract: Disclosed are a molecular detection system and a detection method thereof. The molecular detection system includes a main control device and a plurality of molecular detection devices. The main control device communicates with each of the plurality of molecular detection devices. The main control device is configured to control each of the plurality of molecular detection devices to perform detection. The main control device includes a display module configured to display detection data of each of the plurality of molecular detection devices. The molecular detection devices may perform different types of detections on different types of samples, which greatly expands the application flexibility and the application scenarios while meeting the detection throughput.

Abstract: The present disclosure relates in some aspects to methods, compositions, and kits for profiling biological targets in a sample using rolling circle amplification of circularized probes captured on a substrate.

Abstract: The present application relates to the field of biotechnology, particularly to a plasmid target, a primer probe, a kit and a method for detecting residual host DNA in a cellular or viral formulation. The plasmid target is one or two selected from a plasmid Ori and a KanR gene, the Ori element has a gene sequence of SEQ ID NO. 1, and the KanR gene has a gene sequence capable of encoding a Kan R peptide chain, wherein the Kan R has amino acid sequences of SEQ ID NOs. 2-5. The present application also relates to a primer probe for the plasmid target, a kit, and a applying method.

Abstract: The present disclosure describes methods and devices for detection and quantification of target polynucleotides in a sample using targeting nucleic acids immobilized to predetermined locations of a surface.

Abstract: A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.

Abstract: Techniques are described for identifying a genetic variant in a test sample by comparing sequences reads obtained from the test sample to unique k-mers that are representative of a target genomic region. In one particular aspect, a method is described that includes generating a dictionary of a target genomic region having a set of unique k-mers by: accessing a sequence of the target genomic region, determining a set of k-mers for the target genomic region, comparing the set of k-mers for the target genomic region with one or more sets of k-mers for non-target genomic regions, and selecting the unique k-mers that do not appear in the one or more sets of k-mers for non-target genomic regions. The dictionary can then be used to identify a genetic variant in a test sample by comparing sequences reads obtained from the test sample to the unique k-mers in the dictionary.

Abstract: [OBJECT] To enable a more sensitive and uniform detection of a molecular-related ion in an analysis of an RNA contained in a sample by means of MALDI mass spectrometry. [MEANS FOR SOLVING PROBLEM] A mixed matrix containing 2,4-dihydroxyacetophenone (DHAP) and 2,4,6-trihydroxyacetophenone monohydrate (THAP) is used as a matrix to analyze an RNA contained in a sample by a matrix-assisted laser desorption/ionization mass spectrometer.

Abstract: The present invention provides apparatus and methods for enriching components or cells from a sample and conducting genetic analysis, such as SNP genotyping to provide diagnostic results for fetal disorders or conditions.

Abstract: Sequencing nucleic acids can identify variations associated with presence, susceptibility or prognosis of disease. However, the value of such information can be compromised by errors introduced by or before the sequencing process including preparing nucleic acids for sequencing. Blunting single-stranded overhangs on nucleic acids in a sample can introduce deamination-induced sequencing errors. The disclosure provides methods of identifying and correcting for such deamination-induced sequencing errors and distinguishing them from real sequence variations.

Abstract: The present invention provides methods, compositions and kits for detecting duplicate sequencing reads. In some embodiments, the duplicate sequencing reads are removed.

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain low error rate, low phasing rate, or increased incorporation rate for a second generation ffN under reduced incorporation times, as well as methods and kits using the same.

Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

Abstract: The present disclosure describes methods for analyzing mRNA poly(A) tail sequences with a high resolution to determine poly(A) tail length and heterogeneity. The method involves digesting an mRNA molecule to liberate a poly(A) tail, preparing a chromatographic sample comprising the mRNA poly(A) tails, preparing a second chromatographic sample comprising a reference sequence comprising a mRNA poly(A) tails having a predetermined length, separating the first and second samples by a chromatography method, which result in one or more chromatograms, and determining a sequence length of the mRNA poly(A) tails by comparing the chromatograms of the first and second samples. Chromatography methods for analyzing the poly(A) tail may include ultraviolet size-exclusion chromatography (SEC UV), ultraviolet ion-pair reversed-phase liquid chromatography (IP RP LC UV), ultra high performance liquid chromatography (UPHLC), and combinations thereof.

Abstract: Provided herein are compositions and methods for sorting and/or identifying live cells. The compositions and methods provide for staining of live cells with aptamer so particular cells can be identified within or sorted from a heterogeneous population of live cells and subsequent reversal of the staining to prepare sorted and/or identified cells in their native state.

Abstract: This disclosure provides, among other things, methods for generating and applying therapeutic interventions. The methods involve, for example, (a) sequencing polynucleotides from cancer cells from a subject; (b) identifying and quantifying somatic mutations in the polynucleotides; (c) developing a profile of tumor heterogeneity in the subject indicating the presence and relative quantity of a plurality of the somatic mutations in the polynucleotides, wherein different relative quantities indicates tumor heterogeneity; and (d) determining a therapeutic intervention for a cancer exhibiting the tumor heterogeneity, wherein the therapeutic intervention is effective against a cancer having the profile of tumor heterogeneity determined.

Abstract: The present invention relates to assays for predicting the presence, absence or development of cancer in an individual, particularly breast and ovarian cancer, by determining the methylation status of certain CpGs in a population of DNA molecules in a sample which has been taken from the individual, deriving an index value based on the methylation status of the certain CpGs, and predicting the presence, absence or development of cancer in the individual based on the cancer index value. The invention further relates to a method of treating and/or prevention of cancer in an individual, particularly breast and ovarian cancer, the method comprising assessing the presence, absence or development of cancer in an individual by performing the assays of the invention, followed by administering one or more therapeutic treatments or measures to the individual based on the assessment.

Abstract: The present invention relates to assays for predicting the presence, absence or development of cancer in an individual, particularly ovarian and endometrial cancer, by determining the methylation status of certain CpGs in a population of DNA molecules in a sample which has been taken from the individual, deriving an index value based on the methylation status of the certain CpGs, and predicting the presence, absence or development of cancer in the individual based on the cancer index value. The invention further relates to a method of treating and/or prevention of cancer in an individual, particularly ovarian and endometrial cancer, the method comprising assessing the presence, absence or development of cancer in an individual by performing the assays of the invention, followed by administering one or more therapeutic treatments or measures to the individual based on the assessment.

Abstract: The present disclosure relates to treatment of a subject who has been identified with an increased A2A expression or density of A2AR+ cells.

Abstract: The invention provides methods and compositions for treating urothelial carcinoma in a patient, for example, by administering a treatment regimen that includes a PD-1 axis binding antagonist (e.g., atezolizumab) to the patient as a neoadjuvant or an adjuvant therapy based on the presence or level of ctDNA in a biological sample obtained from the patient. Also provided are compositions (e.g., a PD-1 axis binding antagonist (e.g., atezolizumab), pharmaceutical compositions thereof, kits thereof, and articles of manufacture thereof) for use in treating urothelial carcinoma in a patient.

Abstract: This invention provides a kit or device for detection of prostate cancer and a method for detecting prostate cancer. This invention provides a kit or device for detection of prostate cancer comprising a nucleic acid capable of specifically binding to an miRNA in a sample from a subject or a complementary strand thereof and a method for detecting prostate cancer comprising measuring the miRNA in vitro.

Abstract: The present invention provides methods and kits for the detection of rare genetic mutations in the human KRAS gene. The methods provided herein use specific restriction enzymes that target regions in the 3? end of a region of interest within the human KRAS gene paired with primers designed for the specific amplification of the digested regions of interest to identify samples comprising these rare mutations.

Abstract: This invention relates to molecular systems for detecting a target polynucleotide sequence in a given nucleic acid analyte and methods of use thereof.

Abstract: A method for determining a presence of a pancreatic tumor, assessing a development or risk of development of a pancreatic tumor, and/or assessing a progression of a pancreatic tumor, including determining a presence and/or content of a modification status of a DNA region with gene EBF2 or a fragment thereof in a sample to be tested.

Abstract: The present invention generally relates to methods and agents for identifying and/or classifying microbes (especially bacteria), yeast organisms and filamentous fungi. In particular, the present invention concerns the discovery of unique single nucleotide polymorphisms (SNPs) in bacterial 16S ribosomal RNA (16S rRNA) and yeast organism and filamentous fungi 18S ribosomal RNA, and methods of classifying and/or identifying bacteria, yeast organisms and/or filamentous fungi in a sample based on the presence of one or more of those SNPs. The present invention also concerns probes, primers and kits that are useful in those method.

Abstract: Disclosed herein are methods and kits for quantifying the presence of a microorganism in a sample. Specifically, disclosed are methods for quantifying a pathogen in a sample, such as a food sample, to determine if the levels of pathogen present in the sample are within an acceptable range.

Abstract: The present invention relates to a method for monitoring the effect of an environmental factor on the proteome of a cell. Furthermore, cell populations are provided that comprise multiple cells each comprising an inserted tag sequence in an intron of said cell, wherein the tag is inserted in-frame with the preceding exonic sequence and wherein the intron into which the tag is inserted is different between cells.

Abstract: Disclosed herein are methods and systems comprising obtaining nucleic acid from a sample that was obtained from a subject; capturing and amplifying a target molecule in the nucleic acid using a molecular inversion probe under hybridization conditions; ligating an adapter to create a circular molecule; sequencing the circular molecule to obtain sequence reads; generating a sequencing file comprising the sequence reads of each molecule and a position of each sequence read in a reference genome of a virus; and generating a reporting file for the subject comprising a predicted lineage of the virus in the sample.

Abstract: A method for heat treating a bearing ring includes performing an induction heating treatment and a liquid spraying treatment on a first radial surface so that a radial thickness of the bearing ring is hardened, performing an induction heating treatment on an opposite radial surface so that the temperature near the opposite radial surface reaches 820-950° C., and at the same time spraying liquid on the first radial surface so that the temperature of the first radial surface does not exceed 150° C., spraying liquid on the second radial surface after the induction heating treatment so that the bearing ring forms a hardened second radially outer portion, a hardened second radially inner portion, and a second radially middle portion having a hardness lower than that of the second radially outer portion and the second radially inner portion, and then performing a low-temperature tempering treatment on the bearing ring.

Abstract: The present disclosure provides a high-strength hot-rolled flat steel product and a method of producing such a product. This is achieved by a high-strength hot-rolled flat steel product with high local cold formability, having a tensile strength Rm of at least 760 MPa, a yield point ratio of at least 0.8 and a hole expansion ratio of at least 30%, an elongation at break of at least 10, a measure of cold formability of at least 0.

Abstract: A hot-stretch-reduced electric resistance welded pipe has a base metal portion and a weld portion, the base metal portion has a predetermined chemical composition, a Ti/N value obtained by dividing Ti content by N content is 3.0 or more, in a microstructure of the weld portion, the average grain diameter is 10.0 ?m or less, the area ratio of ferrite is 20% or more, and the remaining structure includes at least one or more of pearlite and bainite/martensite, and in a texture of the weld portion, the accumulation intensity of a {001} plane is 6.0 or less, and a critical cooling rate Vc90 of the base metal portion is 5° C./s to 90° C./s.

Abstract: The high-strength steel sheet has a chemical composition with MSC value in the range of 2.7% to 3.8% by mass defined by a specific formula, wherein the high-strength steel sheet has a microstructure including specific microstructures in a surface layer region extending from the surface of the steel sheet to a depth of 100 ?m and in an inner region other than the surface layer region. The high-strength steel sheet has the maximum height of the surface roughness of 30 ?m or less, a tensile strength of 980 MPa or more, a uniform elongation of 6% or more, and a ratio of 107-cycle plane bending fatigue strength to tensile strength (fatigue limit ratio) of 0.45 or more.

Abstract: Provided is an ultra high strength steel sheet having a high yield ratio, and a method for manufacturing same and, more specifically, to a steel sheet having ultra high strength, a high yield ratio and excellent bendability, and a method for manufacturing same.

Abstract: A cold-rolled flat steel product has a tensile strength of 750-940 MPa, a high strength, an improved weldability and optimized shaping properties, and can be produced at low cost. The cold-rolled flat steel product consists of a steel composed, in percent by mass, of C: 0.040-0.100%; Mn: 2.10-2.50%; Si: 0.10-0.40%; Cr: 0.30-0.90%; Ti: 0.020-0.080%, B: 0.0005-0.0020%; N: 0.003-0.010%; Al: up to 0.10%; Ca: up to 0.005%; P: up to 0.025%; S: up to 0.010%; optionally one or more of the following elements: Mo: up to 0.20%; Nb: up to 0.050%; Cu: up to 0.10%; V: up to 0.020%; Ni: up to 0.10%, the remainder being iron and unavoidable impurities, and total content of impurities is limited to at most 0.5% by mass and contents of phosphorus (“P”) and sulfur (“S”) belong to the impurities.

Abstract: This application pertains to methods of recovering metals from metal sulfides that involve contacting the metal sulfide with an acidic sulfate solution containing ferric sulfate and a reagent that has a thiocarbonyl functional group, wherein the concentration of reagent in the acidic sulfate solution is sufficient to increase the rate of metal ion extraction relative to an acidic sulfate solution that does not contain the reagent, to produce a pregnant solution containing the metal ions.

Abstract: The present invention relates to a method for the biological recovery of metals in electric and electronic waste, which comprises a) inoculating a series of iron-oxidizing microorganisms and a mineral medium or fertilizers into an immobilized biomass column; b) performing a stage of biological oxidation of the iron II ions present in said mineral medium or fertilizers to iron III ions; c) irrigating a liquid phase into leaching columns that house an electrical material or one or more printed circuit boards from which metals are to be recovered, the iron III ions being reduced to iron II oxidizing the metals, and separating the metals by means of the dissolution thereof; and d) extracting the metals from the solution. The invention operates under a cyclic process that allows the iron II solution being re-circulated to the biomass column.

Abstract: A Ni-based alloy consisting of, in terms of mass %: 0.10%<C?0.30%; Si?0.50%; Mn?0.50%; P?0.030%; S?0.010%; Cu?3.00%; 30.0%?Cr?39.0%; Mo?3.00%; Fe?3.00%; 2.00%?Al?5.00%; O?0.0100%; N?0.050%; Nb?0.50%; V?0.50%; Ti?0.50%; Ta?0.50%; W?0.50%; and at least one selected from the group consisting of 0.0010%?B?0.0100%, 0.0010%?Mg?0.0100%, and 0.0010%?Ca?0.0100%, with the balance being Ni and unavoidable impurities, in which the alloy comprises an austenite phase having an average grain diameter of 50.0 ?m or less, a M23C6-type carbide having an average circle equivalent particle diameter of 1.0 ?m or more, and a massive ?-Cr phase having an average circle equivalent particle diameter of 10.0 ?m or less.

Abstract: A 5000-series aluminum alloy expanded material which does not undergo the occurrence of welding cracks even when the aluminum alloy expanded material is subjected to high-speed welding utilizing laser welding or the like; a welding-joined body comprising the aluminum alloy expanded material; and a method for efficiently welding the aluminum alloy expanded material. An aluminum alloy expanded material for welding use, which is characterized by having an Mg content of 0.2% by mass or more and less than 6.0% by mass, and containing a surface active element capable of reducing the surface tension of molten aluminum, in which the surface active element comprises at least one element selected from Sr, Ca, Sb, Li and Ba, and the content of the surface active element is 0.08 to 0.50% by mass inclusive.

Abstract: A sintered cemented carbide includes a high entropy carbide or a spinodal decomposed product thereof; and a metallic binder containing at least one of Co, Co—Ru, Ni, Co—Ni, Co—Cr, Co—Ni—Cr, Co—Re, Co—Ni—Re, Co—Ni—Ru, or a high entropy alloy, wherein the high entropy carbide is a single-phase solid solution carbide comprising four to ten metallic elements, and the spinodal decomposed product thereof includes two chemically distinct phases having a same crystal structure. A sintered cemented carbide also includes a carbide including at least one of WC, TiC, ZrC, HfC, NbC, TaC, or Cr3C2; and a metallic binder including a high entropy alloy. The high entropy alloy is an alloy of four to ten alloy elements selected from Al, Be, Fe, Co, Cr, Ni, Cu, W, V, Zr, Ti, Mn, Hf, Nb, Mo, Ru, Re, Ge, Sn, C, B, or Si.

Abstract: A method for smelting low-phosphorus high-manganese steel based on reduction dephosphorization of ferromanganese is provided in the present application, relating to the technical field of high-manganese steel smelting, where the dephosphorization of ferromanganese is carried out under reducing atmosphere conditions through mediate-frequency induction furnace to obtain molten ferromanganese with lower phosphorus content, which is subsequently mixed with low phosphorus molten steel obtained by smelting in oxidative period of electric arc furnace in LF ladle refining furnace to make the Mn content of steel reach the requirement of high-manganese steel, and smelting is carried out under the condition of reducing atmosphere by adjusting the composition and temperature of the molten steel to meet the requirements of the target composition of the steel grade before tapping the steel.

Abstract: This non-oriented electrical steel sheet has a predetermined chemical composition, one or more particles that are a precipitate of a sulfide or an oxysulfide of one or more selected from the group consisting of Mg, Ca, Sr, Ba, Ce, La, Nd, Pr, Zn, and Cd or both the sulfide and the oxysulfide and have a diameter of more than 0.5 ?m are present in a visual field of 10000 ?m2, and, when EBSD observation is performed on a surface parallel to a steel sheet surface, in a case where a total area is indicated by Stot, an area of {100} orientated grains is indicated by S100, an area of orientated grains in which a Taylor factor M becomes more than 2.8 is indicated by Styl, a total area of orientated grains in which the Taylor factor M becomes 2.8 or less is indicated by Stra, an average KAM value of the {100} orientated grains is indicated by K100, and an average KAM value of the orientated grains in which the Taylor factor M becomes more than 2.8 is indicated by Ktyl, 0.20?Styl/Stot?0.85, 0.05?S100/Stot?0.

Abstract: A steel sheet may be used for hot-dip galvanizing capable of manufacturing hot-dip galvannealed steel sheet(s) with a high Si content and in which alloying unevenness is suppressed. Such a steel sheet for hot-dip galvanizing may include an internal oxide layer containing an oxide of Si between a surface layer of the steel sheet and a steel sheet base portion. The Si content in a chemical composition of the steel sheet may be 1.0 mass % or more. A solid solution Si amount from a surface of the steel sheet to a depth of 1 ?m measured at all four positions including positions of 10 mm, 30 mm, and 50 mm from an edge in a coil width direction and a position of a center in the coil width direction at a rear end in a rolling direction of the steel sheet for hot-dip galvanizing may be 1.4 wt. % or less.

Abstract: A martensitic steel, consisting of, in % in weight: C: 0.30 to 0.80%, Si: 2.50 to 4.50%, Mn: 1.00 to 2.50%, Al: 0.40 to 1.50%, Cr: 0.1 to 2.00%, V: 0.01 to 0.40%, Ti: 0.005 to 0.35%, and optionally one or more of Nb: less than 0.35%, Zr: less than 0.35%, Ta: less than 0.35%, P: less than 0.25%, S: less than 0.25%, Co: less than 0.50%, Mo: less than 0.90%, W: less than 0.90%, Ni: less than 0.50%, Cu: less than 0.50%, N: less than 0.050%, Ca: less than 0.10%, Mg: less than 0.10%, Ce: less than 0.10%, La: less than 0.10%, B: less than 0.10%, the balance Fe and impurities, and comprising one or more intermetallic phases based on an Al—Fe—Mn—Si system.

Abstract: A corrosion resistant duplex stainless steel (ferritic austenitic alloy) is suitable for use in a plant for the production of urea. Uses of the corrosion resistant duplex stainless steel include in objects made of said duplex stainless steel, in methods for the production of urea, in a plant for the production of urea comprising one or more parts made from said duplex stainless steel, and in methods of modifying an existing plant for the production of urea. In one aspect, the composition consists of in weight % (wt %): C 0.010 to 0.015; S 0.08 to 0.23; Mn 1.0 to 1.05; Cr 29.07 to 29.92; Ni 5.76 to 7.17; Mo 3.0 to 4.0; W max 2.55; N 0.3 to 0.35; Cu max 0.01; S max 0.008; P max 0.008; at least one of Ti, Nb, Hf, Ca, Ba, V, and B max 0.5 total; balance Fe and unavoidable occurring impurities.

Abstract: This steel sheet has a predetermined chemical composition, and has, at a t/4-position which is a position at a distance of t/4 from the surface in a sheet thickness direction cross section when the sheet thickness is defined as t, a metal structure including 20% or more of ferrite and a total of 40% or more of bainite and martensite in volume fraction, the remaining portion being at least one selected from retained austenite and perlite. In a square-shaped area in which the length of one side is t/4 and which is centered at the t/4-position in the sheet thickness direction cross section, when the Mn concentration is measured at multiple measurement points located apart at a 1 ?m-interval, the proportion of measurement points in which the Mn concentration is at least 1.1 times of the average value of the Mn concentration at all the measurement points is less than 10.0%. The steel sheet has a tensile strength of 980 MPa or more.

Abstract: A medium heavy alloy (MHA) and a fabrication method thereof are disclosed. The MHA includes a composition designed and processed such that the MHA has properties comprising a tensile strength of about 1527 MPa, a proof strength of about 1337 MPa, and an impact toughness of about 180 J, when the MHA is forged, and the tensile strength of about 1746 MPa, the proof strength of about 1571 MPa, and the impact toughness of about 55 J, when the MHA is agedly treated. The method includes providing a composition designed according to design specifications of the MHA; forming a cast alloy from the composition by a metallurgy process; and forged the cast alloy at a first temperature to form the MHA having properties that are the design specifications.

Abstract: High strength plated steel sheet having high hydrogen embrittlement resistance, that is, alloyed hot dip galvannealed steel sheet including steel sheet containing C: 0.05 to 0.40%, Si: 0.2 to 3.0%, Mn: 0.1 to 5.0%, and sol. Al: 0.4 to 1.50% and a alloyed hot dip galvannealed layer deposited on at least one surface of the steel sheet to 10 to 100 g/m2 and containing Fe: 5.0 to 15.0% and Al: 0.01 to 1.0%, having an internal oxidation layer including grain boundary oxides in a surface layer of the steel sheet, when examining a cross-section of a surface layer of the steel sheet, a Ratio A of the length of the grain boundary oxides projected an interface of the steel sheet and the alloyed hot dip galvannealed layer to the length of interface is 50% or more and 100% or less, and a surface depleted layer with a steel composition not including the grain boundary oxides which satisfies, by mass %, Si?0.6% and Al?0.05% is included at a depth of ½ of the average depth of the internal oxidation layer.