Abstract: A device and a method are disclosed for collecting and culturing fish embryos and evaluating combined toxicity of thiamethoxam and tetraconazole. An embryo collection component and an embryo culture component are provided on a base plate. The embryo collection component includes a collection tray detachably connected to a bottom of a culture tube. The embryo culture component includes a cavity plate with a stopper inside and a top plate provided with a culture well. A bottom of the culture well is provided with a leakage hole; and after the top plate is installed into the cavity plate, the stopper occludes the leakage hole. The new device is used to carry out the combined toxicity effect test of thiamethoxam and tetraconazole on zebrafish embryos, which can be used to avoid development of pesticide mixtures that have a good preventive effect but pose increased toxicity risk to the ecological environment.

Abstract: The method for rearing an animal according to the present invention includes: grasping a tide-generating force; and feeding, to the animal, foods varying in fat ingredient content according to the variation level of the tide-generating force. In addition, the apparatus for rearing an animal according to the present invention is provided with a food feeding means for feeding, to the animal, foods varying in fat ingredient content according to the variation level of a tide-generating force.

Abstract: A system of harvesting sea creatures from a trench holding the sea creatures in seawater, including a trench and a harvesting apparatus that extracts the sea creatures from the trench. The trench includes a gate at one end of the trench. The gate insertable into an opening at the end of the trench to prevent seawater from entering or exiting the trench and removable from the opening to allow the seawater to enter or exit the trench. The harvesting apparatus includes a conveyor that inclines. The conveyor includes a conveyor belt and a wedge at one end of the conveyor that contacts the bottom floor of the trench when the conveyor is inclined. The wedge directs the sea creatures onto the conveyor so that the sea creatures are conveyed by the conveyor belt out of the trench.

Abstract: Disclosed is an artificial reef molded from a mixture of environmentally friendly homogenized organic and inorganic materials, cement, and 5 aggregates of sand and gravel to form thereon an artificial reef such as reef buds, reef plates, reef honeycombs and other reef structures.

Abstract: The shrimp culturing system includes a culturing tank and a clarifier tank. A plurality of shrimp cubes are suspended in the culture tank by set pairs of support bars. Each shrimp cube includes a plurality of stacked frames set at predetermined intervals. Each frame supports a mesh therein providing living space for the shrimp, and each stack is interconnected by ropes, which connect to the support bars to be suspended thereby. The shrimp cubes provide maximal space for culturing shrimp. The waste water from the culture tank cycles into the clarifier tank, which nurtures a biofloc to consume the waste and assimilate nitrogen, thereby producing their own waste that can be consumed by the shrimp. Excess solid waste drains via a solids removal manifold, and clarified water is airlifted back into the culture tank. A post-larval tank can be stacked onto the clarifier tank to introduce new batches of shrimp.

Abstract: This invention is directed to methods for manipulating sex, reversing sex, changing sex ratio, or moderating crustacean androgenic gland peptide expression in crustaceans for producing a monosex culture. In one embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a male crustacean. In another embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a female crustacean that had acquired a spermatophore (a sperm sac) after mating with a male. In another embodiment, the present invention provides a method of producing a monosex culture of crustacean by introducing a recombinant IAG peptide into the female crustacean, thereby obtains male with altered sexual characteristics for mating with a normal female. In one embodiment, the crustacean is a shrimp or lobster.

Abstract: Cysts, in particular Artemia cysts, are incubated the in a hatching medium so that a portion of the cysts hatch and release free swimming live food organisms. The free swimming live food organisms have subsequently to be separated from the cysts which have not hatched. Before incubating the cysts, magnetic particles are applied onto the cysts so that these cysts can be attracted magnetically, in particular in the liquid hatching medium. This enables to separate the unhatched cysts and the empty cyst shells, coated with the magnetic particles, effectively from the free swimming live food organisms which do not have magnetic particles applied onto their outer surface. The invention also relates to the coated cysts.

Abstract: The present invention relates to methods of raising crustaceans, comprising maintaining crustaceans in low salinity water having a Calcium-sensing Receptor (CaSR) modulator(s), and adding feed that contains an agent that contributes to modulation of a crustacean CaSR to the water.

Abstract: The present invention relates to a nucleic acid molecule encoding an insulin-like factor of the androgenic gland of the freshwater prawn Macrobrachium rosenbergii (M. rosenbergii). The present invention further relates to methods of silencing the expression of the insulin-like factor gene in decapod crustaceans order, particularly in M. rosenbergii, useful for producing male monosex populations.

Abstract: A method of improving the reproductive performance of penaeus shrimps comprises a step of treating a fresh diet with sodium alginate and a step of feeding female penaeus shrimps with the treated fresh diet to enhance the breeding ability of female penaeus shrimps, also promote growth and survival of the subsequent larvae of the female penaeus shrimps.

Abstract: A method of improving the reproductive performance of penaeus shrimps comprises a step of treating a fresh diet with sodium alginate and a step of feeding female penaeus shrimps with the treated fresh diet to enhance the breeding ability of female penaeus shrimps, also promote growth and survival of the subsequent larvae of the female penaeus shrimps.

Abstract: The present invention relates to methods of raising crustaceans, comprising maintaining crustaceans in low salinity water having a Calcium-sensing Receptor (CaSR) modulator(s), and adding feed that contains an agent that contributes to modulation of a crustacean CaSR to the water.

Abstract: The present invention relates to methods of raising crustaceans, comprising maintaining crustaceans in low salinity water having a Calcium-sensing Receptor (CaSR) modulator(s), and adding feed that contains an agent that contributes to modulation of a crustacean CaSR to the water.

Abstract: An agitator is provided including an open system having a base holding a plurality of culturing beakers as well as a lid holding a tube transporting fresh seawater into each beaker, gently stirring the water within and eliminating any decaying embryos. Each beaker has at least a bottom made of mesh allowing water to flow through. The agitator can be used to culture any fragile planktonic organisms that require an extended rearing time.

Abstract: This invention is about triploid breeding method for different shrimp species especially to the triploid production both in cold-warm water shrimp and tropical species, including the induction of MI triploid shrimp, the triploid induction methods for different shrimp and the development and utilization of revulsants for triploid shrimp. The triploid of cold-water shrimps were obtained by collecting the fertilized eggs and heat shock treatment; while the triploid induction of tropical shrimps were obtained with collection of the fertilized eggs, treatment of the revulsants or cold shock.

Abstract: This invention is about triploid breeding method for different shrimp species especially to the triploid production both in cold-warm water shrimp and tropical species, including the induction of MI triploid shrimp, the triploid induction methods for different shrimp and the development and utilization of revulsants for triploid shrimp. The triploid of cold-water shrimps were obtained by collecting the fertilized eggs and heat shock treatment; while the triploid induction of tropical shrimps were obtained with collection of the fertilized eggs, treatment of the revulsants or cold shock.

Abstract: The inventions of the application relates to a method and device for detecting the oviposition of crustaceans, which comprises sealing off one of the two gonopores of a female crustacean, covering the other gonopore with a bag, and detecting eggs laid into the bag with a actor. By using this method and device, unfertilized eggs of crustaceans can be easily obtained, thus facilitating the culture and strain improvement of crustaceans.

Abstract: Incubation device (10) has a structure wherein an incubation tank (11) and a separation tank (12) are put into communication with each other at the lowermost parts via a narrow communicating port (13). A side opening (14) is formed in the separation tank (12). The wall of the lower part of an upper housing part (12a) and of the entirety of a lower housing part (12b) of the separation tank (12) are formed from a colored, light-shielding material OP. Sea water (D) is housed in incubation tank (11) and the lower part of separation tank (12), freshwater (E) is housed in the upper part of the separation tank (12), and an interface (F) between the sea water (D) and the freshwater (E) is formed inside the separation tank (12). Brine shrimp larvae (2) hatch in the incubation tank (11) and upon moving into the separation tank (12), move from the light-shielded lower part towards the unshielded upper part and eventually swim outside into an aquarium tank from the side opening (14).

Abstract: The present invention relates to a recirculating marine aquaculture process for production of a crab species, including (i) a broodstock conditioning, (ii) spawning, (iii) egg incubation, (iv) larval growth, (v) nursery post-larval growth, and (vi) grow-out of the crab to a final product weight, in which each stage (i)-(vi) of the process involves operation in an aqueous medium that is coupled in liquid recirculation relationship with waste component removal from the aqueous medium and returning purified aqueous medium to the external environment. The process involves operation in a closed, recirculating aquaculture system in which photoperiod, water temperature, water chemistry, and diet are optimized and then continuously monitored and controlled to obtain optimal production at each of the six phases (i)-(vi) of the life cycle.

Abstract: A crustacean larva raising method includes the steps of providing a tank (50) to hold larva raising medium, continuously supplying sterilized, filtered larva raising medium to the tank (50) through outlets (64) adapted to cause horizontal circulation of the medium and at an outlet flow velocity preventing larva damage, continuously draining the medium through a drain assembly (65) including a larva screen and maintaining the medium at a selected temperature. Apparatus to carry out the crustacean larva raising method comprises of modular channel sections (51, 52) of the tank (50), which are bolted and sealed together, two sub tanks (53, 54), a UV sterilizer unit (55) incorporating temperature control means and a submersible pump (56) to circulate filtered and pre-sterilized medium. A crustacean larva feeding composition and a method for preparing such a composition are also provided.

Abstract: Disclosed is a process for growing the microflora Thraustochytrium, Schizochytrium, and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment of the present invention, the process produces microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further disclosed is a food product which includes Thraustochytrium, Schizochytrium, and mixtures thereof, and a component selected from flaxseed, rapeseed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids. The lipids containing these fatty acids can also be extracted and used in nutritional, pharmaceutical and industrial applications.

Abstract: A method of dissipating heat from the interior of containers of harvested brine shrimp eggs to prevent overheating from spontaneous or environmental sources. Hollow convective members are installed embedded within the mass of eggs within the containers. The method helps in the use of larger, more efficient bags for brine shrimp eggs without increased mortality rates of the eggs.

Abstract: A method for incubating eggs or larvae of fish, crustaceans, or related organisms. The eggs or larvae are located in depressions in the surface of a plate formed of an aqueous polymeric gel, and the surface is sealingly covered with a porous membrane capable of gas transport therethrough. The plate sealed with the membrane is placed in at least intermittent contact with water during the incubation period, and the eggs or larvae are separated from the plate and membrane after incubation is complete.