Abstract: The present invention provides improved methods of injecting an avian egg containing an embryo, preferably an early embryo (e.g., a blastoderm). The methods of the invention may be used to deliver a substance to an egg, remove a sample from an egg, and/or to insert a detector device into an egg to collect information therefrom. In preferred embodiments, the invention is used to deliver a substance to the embryo in ovo. In other preferred embodiments, the invention is used to produce chimeric or transgenic avian embryos in ovo.

Abstract: The present invention relates to the field of avian reproduction. In particular, the present invention provides a method of activating an egg in a shell. The invention also provides a method of activating an egg in a shell, whereby a live chick is hatched.

Abstract: A pneumatically operated egg injection machine includes a sealed frame structure with a pair of in line parallel tracks through an injection section and a transfer section in series. An injection assembly over one parallel track includes a plurality of injectors gripped in a support plate to simultaneously inject vaccine into the same injection region irrespective of egg height and orientation. Fluid delivery systems meter prescribed vaccine dosages to the injecting needles with reduced turbulence, friction, heat and residence time to increase the delivered titer to the injected eggs. A transfer assembly includes a plurality of transfer suction cups which lift the injected eggs by causing a reduced pressure in a ring around the injection hole while maintaining the injection hole at atmospheric pressure, thus avoiding negative pressure in the egg. Once the eggs are lifted, the plate and suction cups move horizontally across the machine over to the other parallel track to deposit the injected eggs.

Abstract: Methods an apparatus for accurately and reliably positioning a device within an egg are provided. An opening is formed within a portion of the shell of an egg and a device is extended through the opening. The device includes a needle having a lumen containing a fluid under pressure. Fluid pressure is monitored and, in response to detecting a change in pressure of the fluid, movement of the device is stopped. Alternatively, fluid flow is monitored and, in response to detecting fluid flow from the needle, movement of the device is stopped. The device may be a substance delivery device that is configured to deliver a substance into the subgerminal cavity of the egg. The device may be a substance removal device that is configured remove a substance from an egg.

Abstract: A method of rearing a bird of germ-free status comprises housing a bird as a parent bird, surgically removing an egg in its shell from the parent bird prior to transfer of the egg to the cloaca in the parent bird, incubating the egg still in its shell and hatching the egg to produce a laying bird. The production of avian eggs of germ-free status is also provided.

Abstract: A pneumatically operated egg injection machine includes a sealed frame structure with a pair of in line parallel tracks through an injection section and a transfer section in series. An injection assembly over one parallel track includes a plurality of injectors gripped in a support plate to simultaneously inject vaccine into the same injection region irrespective of egg height and orientation. Fluid delivery systems meter prescribed vaccine dosages to the injecting needles with reduced turbulence, friction, heat and residence time to increase the delivered titer to the injected eggs. A transfer assembly includes a plurality of transfer suction cups which lift the injected eggs by causing a reduced pressure in a ring around the injection hole while maintaining the injection hole at atmospheric pressure, thus avoiding negative pressure in the egg. Once the eggs are lifted, the plate and suction cups move horizontally across the machine over to the other parallel track to deposit the injected eggs.

Abstract: An egg is accelerated such that the inner and outer shell membranes separate and allow the air cell to move within the egg when the egg is repositioned. By separating the outer and inner shell membranes of an egg, the air cell will reposition itself at the upper portion of the egg shell. An egg may be accelerated in various ways according to embodiments of the present invention including, but not limited to, agitation, “flicking” by hand, spinning, rotating, centrifuging, etc.

Abstract: A water soluble sanitizing composition includes a chlorinated isocyanurate and an effervescent alkali base, the effervescent base including sodium carbonate agent, adipic acid and sodium bicarbonate in a weight ratio of approximately 1:1:1 to provide a sanitizing solution having a pH of from 6.8 to 7.4. A method for sanitizing a product such as in-ovo injection equipment includes the step of dissolving the composition in water to form a sanitizing solution and flushing the in-ovo injection equipment with the sanitizing solution prior to each in-ovo injection.

Abstract: Methods and apparatus for processing eggs based upon a characteristic such as gender are provided. Material is extracted from each of a plurality of live eggs, the extracted material is assayed to identify eggs having the characteristic, and then eggs identified as having the characteristic are processed accordingly.

Abstract: In ovo injection apparatus include an egg carrier configured to hold a plurality of eggs and to provide external access to the eggs, a plurality of injection devices positioned above the carrier, and an egg support assembly positioned beneath the carrier that is configured to support each egg in the carrier during contact therewith by a respective injection device. The egg support assembly includes a frame, a plate having an array of openings attached to the frame, and a plurality of pedestals removably secured within a respective one of the openings. The egg support assembly is operatively associated with the plurality of injection devices such that each pedestal moves upwardly through a respective opening in the carrier to support an egg as a respective injection device makes contact with the egg.

Abstract: A method for treating a fertilized egg of a bird, a method for increasing the body weight of a chick at hatch, and a method for increasing the hatchability of a fertilized egg of a bird, comprising injecting, into the fertilized egg after start of incubation, a solution containing amino acids which determine the nutritive value of proteins in the fertilized egg; a method for hatching a fertilized egg of a bird, comprising hatching the fertilized egg after start of incubation treated according to the treatment method; a fertilized egg of a bird after start of incubation, the egg being treated according to the treatment method; and a bird hatched from the fertilized egg.

Abstract: Methods and apparatus for identifying live eggs utilize a cup having a photodetector therewithin that overlies a portion of an egg. The egg is illuminated via a light source positioned adjacent the egg. Variations in the intensity of light passing through the egg is detected via the photodetector which generates an output signal corresponding to detected light intensity. The output signal is processed to identify cyclical and non-cyclical variations in light intensity. The egg is designated as a live egg in response to identifying cyclical and/or non-cyclical variations in light intensity.

Abstract: Methods of pooling or localizing the allantoic fluid present in avian shell eggs, so that the fluid may be sampled, assessed or injected, are described. The methods involve tilting the avian shell egg so that the major axis of the egg is from about 10 to 180 degrees from the vertically upright position, so that the allantois pools under the uppermost portion of the egg shell. The allantoic fluid may then be sampled, or a substance injected into the allantois, by a vertical needle or probe traveling in a downward direction.

Abstract: A method for making a quality determination in avian eggs, such as relating to fertility or hatching or hatchling viability, comprises the following activities. A process line is equipped to process an endless succession of eggs at an early opportunity. The process line has an ultrasound inspection station for the eggs. The ultrasound inspection results are analyzed to make a finding correlatable to the egg's shell quality, which in turn is correlatable to such quality factors as fertility or hatching or hatchling viability. A sorting determination is made based on this analysis as to which output category the egg should be sorted. The output categories might number three or so including qualified premium as for graduation to hatchery operation, not qualified for hatchery but not waste, and flunked because unusable and hence waste. The intermediate category might include graded for pet consumption.

Abstract: The invention provides a method of rearing a bird of germ-free status. The method comprises housing a bird as a parent bird, surgically removing an egg in its shell from the parent bird prior to transfer of the egg to the cloaca in the parent bird, incubating the egg still in its shell and hatching the egg to produce a laying bird. The invention also relates to the production of avian eggs of germ-free status.

Abstract: The present invention provides methods for exposing an avian embryo to light during incubation. The methods may be used to increase a bird's weight, increase muscle weight in a bird, and decrease the mortality rate of a bird.

Abstract: A pneumatically operated egg injection machine includes a sealed frame structure with a pair of in line parallel tracks through an injection section and a transfer section in series. An injection assembly over one parallel track includes a plurality of injectors gripped in a support plate to simultaneously inject vaccine into the same injection region irrespective of egg height and orientation. Fluid delivery systems meter prescribed vaccine dosages to the injecting needles with reduced turbulence, friction, heat and residence time to increase the delivered titer to the injected eggs. A transfer assembly includes a plurality of transfer suction cups which lift the injected eggs by causing a reduced pressure in a ring around the injection hole while maintaining the injection hole at atmospheric pressure, thus avoiding negative pressure in the egg. Once the eggs are lifted, the plate and suction cups move horizontally across the machine over to the other parallel track to deposit the injected eggs.

Abstract: In ovo injection apparatus include an egg carrier configured to hold a plurality of eggs and to provide external access to the eggs, a plurality of injection devices positioned above the carrier, and an egg support assembly positioned beneath the carrier that is configured to support each egg in the carrier during contact therewith by a respective injection device. The egg support assembly includes a frame, a plate having an array of openings attached to the frame, and a plurality of pedestals removably secured within a respective one of the openings. The egg support assembly is operatively associated with the plurality of injection devices such that each pedestal moves upwardly through a respective opening in the carrier to support an egg as a respective injection device makes contact with the egg.

Abstract: Methods and apparatus for identifying live eggs utilize a cup having a photodetector therewithin that overlies a portion of an egg. The egg is illuminated via a light source positioned adjacent the egg. Variations in the intensity of light passing through the egg is detected via the photodetector which generates an output signal corresponding to detected light intensity. The output signal is processed to identify cyclical and non-cyclical variations in light intensity. The egg is designated as a live egg in response to identifying cyclical and/or non-cyclical variations in light intensity.

Abstract: The invention provides a method of rearing a bird of germ-free status. The method comprises housing a bird as a parent bird, surgically removing an egg in its shell from the parent bird prior to transfer of the egg to the cloaca in the parent bird, incubating the egg still in its shell and hatching the egg to produce a laying bird. The invention also relates to the production of avian eggs of germ-free status.

Abstract: Systems and methods for rapidly identifying live eggs within a stream of eggs with high accuracy, are provided. A stream of eggs are candled via a candling apparatus and each candled egg is designated as being either live, non-live, or “uncertain”. Eggs designated as non-live and uncertain are removed from the stream. Each egg designated as uncertain is “recandled” at a separate station via an additional candling procedure in order to definitively determine if the “uncertain” egg is live or non-live. Uncertain eggs identified as being live via recandling are returned to the egg stream such that the stream contains only live eggs. The uncertain eggs determined to be non-live via recandling are removed and are either discarded or used for other purposes.

Abstract: To provide a producibility improver for poultry, comprising a polymannose having a molecular weight distribution in which a polymannose having the molecular weights ranging from 1.8×103 to 1.8×105 accounts for 70% or more; the producibility improver for poultry further comprising a polyphenol compound; the producibility improver for poultry further comprising a delipidated rice bran; and a method of improving producibility for laying hens or edible chicken, using any one of the producibility improvers. According to the present invention, the improvement of producibility for poultry can be made at low costs.

Abstract: A method of introducing a substance into an avian egg such that potential contamination is substantially reduced includes removing an avian egg containing a live avian embryo from an incubator; applying a sanitizing fluid to the shell of the egg to kill pathogens attached thereto; forming an opening in the shell; inserting an injection device through the opening; releasing a substance into the egg via the needle; retracting the injection device from the egg; and applying a sanitizing fluid to the needle to kill pathogens attached thereto. Prior to injection, the sanitizing fluid is applied to substantially the entire surface of the egg shell.

Abstract: The present invention provides methods for the production of avian gametes, comprising reducing endogenous primordial germ cells (PGCs) in a recipient bird in ovo and introducing donor primordial germ cells from another bird into the recipient bird in ovo. The present invention further provides a method of increasing the ratio of male offspring from a female bird, comprising reducing endogenous PGCs in the female bird in ovo, introducing donor PGCs from another bird into the female bird in ovo, and incubating the female bird to hatch. The female bird is raised to sexual maturity and bred to produce a plurality of eggs having a higher proportion of male eggs than in the absence of the inventive methods. In some embodiments, the donor PGCs are from a different species than the recipient bird. In preferred embodiments, endogenous PGCs are reduced using busulfan.

Abstract: Methods and apparatus for delivering predetermined dosages of fluid to egg injection devices are provided and include drawing fluid from a fluid source into a syringe barrel via inlet tubing in fluid communication with the fluid source and with the syringe barrel, and dispensing a predetermined dosage of fluid from the syringe barrel to an egg injection device via outlet tubing in fluid communication with the egg injection device and with the syringe barrel. The outlet tubing is closed (e.g., pinched closed) during the drawing step, and the inlet tubing is closed (e.g., pinched closed) during the dispensing step.

Abstract: Methods of pooling or localizing the allantoic fluid (16) present in avian shell eggs (10), so that the fluid may be sampled, assessed or injected, are described. The methods involve tilting the avian shell egg so that the major axis of the egg is from about 10 to 180 degrees from the vertically upright position, so that the allantois pools under the uppermost portion of the egg shell. The allantoic fluid may then be sampled, or a substance injected into the allantois, by a vertical needle (17) or probe traveling in a downward direction.

Abstract: A method for making a quality determination in avian eggs, such as relating to fertility or hatching or hatchling viability, comprises the following activities. A process line is equipped to process an endless succession of eggs at an early opportunity. The process line has an ultrasound inspection station for the eggs. The ultrasound inspection results are analyzed to make a finding correlatable to the egg's shell quality, which in turn is correlatable to such quality factors as fertility or hatching or hatchling viability. A sorting determination is made based on this analysis as to which output category the egg should be sorted. The output categories might number three or so including qualified premium as for graduation to hatchery operation, not qualified for hatchery but not waste, and flunked because unusable and hence waste. The intermediate category might include graded for pet consumption.

Abstract: Methods and apparatus for delivering predetermined dosages of fluid to egg injection devices are provided and include drawing fluid from a fluid source into a syringe barrel via inlet tubing in fluid communication with the fluid source and with the syringe barrel, and dispensing a predetermined dosage of fluid from the syringe barrel to an egg injection device via outlet tubing in fluid communication with the egg injection device and with the syringe barrel. The outlet tubing is closed (e.g., pinched closed) during the drawing step, and the inlet tubing is closed (e.g., pinched closed) during the dispensing step.

Abstract: An apparatus for injecting fluid substances into eggs is disclosed, comprising a plurality of injectors, needles disposed within each of the injectors moveable between a retracted position and an extended injecting position with respect to the injectors, and a bridge assembly for positioning the injectors in alignment with a corresponding plurality of eggs in an egg flat. The injectors rest substantially vertically in openings in a horizontal plate. The openings in the plate are slightly larger than the cross-section of the injectors for permitting the injectors to move vertically within the openings in the plate with respect to the plate. An egg nesting cup is pivotally secured to the lower end of each injector. The injectors are lowered to engage the eggs. When the plate and the injectors are lowered the nesting cups seat against the eggs and the injectors move vertically upward with respect to the plate.

Abstract: Methods and apparatus for forming an opening in a shell of an avian egg such that the potential for cracking is greatly reduced are provided and comprise moving a punch through an egg shell at speeds equal to or greater than thirty (30) inches per second. Punches may be driven via various devices including gravity-assisted devices, spring-assisted devices, solenoid-assisted devices, and fluid-assisted (e.g., compressed air, compressed nitrogen, liquid, etc.) devices. The shell of an egg may be pierced without causing cracking by hurling one or more small projectiles at the egg shell at speeds in excess of 30 inches per second. A punch may be mounted on a piezoelectric actuator configured to vibrate at frequencies in a range of between about 1,000 Hz and about 100,000 Hz. These frequencies effectively cause a punch to move at speeds in excess of 30 inches per second.

Abstract: Methods and apparatus for forming an opening in a shell of an avian egg such that the potential for cracking is greatly reduced are provided and comprise moving a punch through an egg shell at speeds equal to or greater than thirty (30) inches per second. Punches may be driven via various devices including gravity-assisted devices, spring-assisted devices, solenoid-assisted devices, and fluid-assisted (e.g., compressed air, compressed nitrogen, liquid, etc.) devices. The shell of an egg may be pierced without causing cracking by hurling one or more small projectiles at the egg shell at speeds in excess of 30 inches per second. A punch may be mounted on a piezoelectric actuator configured to vibrate at frequencies in a range of between about 1,000 Hz and about 100,000 Hz. These frequencies effectively cause a punch to move at speeds in excess of 30 inches per second.

Abstract: The present invention provides methods for the production of avian gametes, comprising reducing endogenous primordial germ cells (PGCs) in a recipient bird in ovo and introducing donor primordial germ cells from another bird into the recipient bird in ovo. The present invention further provides a method of increasing the ratio of male offspring from a female bird, comprising reducing endogenous PGCs in the female bird in ovo, introducing donor PGCs from another bird into the female bird in ovo, and incubating the female bird to hatch. The female bird is raised to sexual maturity and bred to produce a plurality of eggs having a higher proportion of male eggs than in the absence of the inventive methods. In some embodiments, the donor PGCs are from a different species than the recipient bird. In preferred embodiments, endogenous PGCs are reduced using busulfan.

Abstract: The present invention relates to the field of avian egg fertilization. In particular, the present invention provides a method of fertilizing an egg in a shell. The invention also provides a method of fertilizing an egg in a shell, whereby a live chick is hatched. The invention also provides developmentally early stage oviposited avian eggs.

Abstract: The invention provides a method of rearing a bird of germ-free status. The method comprises housing a bird as a parent bird, surgically removing an egg in its shell from the parent bird prior to transfer of the egg to the cloaca in the parent bird, incubating the egg still in its shell and hatching the egg to produce a laying bird. The invention also relates to the production of avian eggs of germ-free status.

Abstract: A pneumatically operated egg injection machine includes a sealed frame structure with a pair of in line parallel tracks through an injection section and a transfer section in series. An injection assembly over one parallel track includes a plurality of injectors gripped in a support plate to simultaneously inject vaccine into the same injection region irrespective of egg height and orientation. Fluid delivery systems meter prescribed vaccine dosages to the injecting needles with reduced turbulence, friction, heat and residence time to increase the delivered titer to the injected eggs. A transfer assembly includes a plurality of transfer suction cups which lift the injected eggs by causing a reduced pressure in a ring around the injection hole while maintaining the injection hole at atmospheric pressure, thus avoiding negative pressure in the egg. Once the eggs are lifted, the plate and suction cups move horizontally across the machine over to the other parallel track to deposit the injected eggs.

Abstract: Apparatus for repositioning eggs from a generally vertical position to a generally horizontal position such that injection/removal of material into/from the side of the egg can occur. Each apparatus may include a cradle, an alignment member, and an orientation member. The cradle has an inclined, arcuate surface with an upper portion, a lower portion, and opposite side portions. The alignment member is operably positioned adjacent the cradle and is configured to engage an egg positioned on the arcuate surface lower portion in an inclined orientation and to releasably secure the egg in a predetermined alignment relative to the cradle. The orientation member is operably positioned relative to the cradle, and is configured to urge an egg positioned on the lower portion in an inclined orientation to a generally vertical orientation after injection/removal of material into/from the egg has occurred.

Abstract: Methods of pooling or localizing the allantoic fluid present in avian shell eggs, so that the fluid may be sampled, assessed or injected, are described. The methods involve tilting the avian shell egg so that the major axis of the egg is from about 10 to 180 degrees from the vertically upright position, so that the allantois pools under the uppermost portion of the egg shell. The allantoic fluid may then be sampled, or a substance injected into the allantois, by a vertical needle or probe traveling in a downward direction.

Abstract: The present invention provides a quick disconnect, disposable needle and hub assembly that can be used in apparatuses used to inject vaccines or other beneficial materials into eggs. In particular, the components of the assembly are manufactured from a polymeric material and the connections between the components in the assembly are by luer lock connections.

Abstract: Methods and apparatus for processing eggs based upon a characteristic such as gender are provided. Material is extracted from each of a plurality of live eggs, the extracted material is assayed to identify eggs having the characteristic, and then eggs identified as having the characteristic are processed accordingly.

Abstract: A method of manipulating an egg or an embryo therein, preferably a chicken egg or embryo, and sealing the egg shell with a heat melted composition. The present invention provides a method whereby a hole in an egg shell may be optionally resealed with a patch from an egg shell membrane and covered by a heat sealed composition. The present invention further provides a method to expel air introduced into the egg during manipulation by expanding the air sac of the egg.

Abstract: A method of altering the phenotype of a bird comprises introducing a DNA molecule into the muscle tissue of a bird contained within an egg during in ovo incubation. The DNA molecule is selected to be effective to cause a change in phenotype, such as an increase in growth rate, feed efficiency, immune response, or a combination of these and other phenotypes in the bird after hatch. A DNA molecule may further be selected to increase disease resistance, induce disease prevention, or neutralize maternal antibodies by the expression of an antigen over a period of time.

Abstract: The present invention provides a method of reducing cholesterol in animals and food products derived from such animals. The method includes feeding to the animals a low phytate food source so that the phytate phosphorus intake of the animal is reduced.

Abstract: The development of oviparous species such as birds after hatch is enhanced by feeding a subject prior to hatch. The subject is fed with a nutrient composition and/or an enteric modulator. A preferred nutrient composition contains at least one protein, peptide, amino acid or carbohydrate; a preferred enteric modulator is hydroxymethylbutyrate. Feeding may be accomplished by administering the nutrient composition and/or enteric modulator into the amnion where it is then orally ingested by the subject.

Abstract: A method for the production and collection of avian sperm comprises the steps of: providing primordial germ cells from a donor avian species; administering the primordial germ cells to a recipient avian species in ovo; incubating the recipient avian species to hatch; and then collecting sperm of the donor avian species from the recipient avian species. For example, the donor avian species may be a whooping crane, and the recipient avian species may be a sand hill crane. In another example, the donor avian species may be a turkey, and the recipient avian species may be a chicken.

Abstract: An apparatus for injecting fluid substances into eggs is disclosed, comprising a plurality of injectors, needles disposed within each of the injectors moveable between a retracted position and an extended injecting position with respect to the injectors, and a bridge assembly for positioning the injectors in alignment with a corresponding plurality of eggs in an egg flat. The injectors rest substantially vertically in openings in a horizontal plate. The openings in the plate are slightly larger than the cross-section of the injectors for permitting the injectors to move vertically within the openings in the plate with respect to the plate. An egg nesting cup is pivotally secured to the lower end of each injector. The injectors are lowered to engage the eggs. When the plate and the injectors are lowered the nesting cups seat against the eggs and the injectors move vertically upward with respect to the plate.

Abstract: The present invention provides a new antigenic type of chicken anaemia viruses (CAV). The CAV according to this invention is isolatable from turkeys in the field and its virulence for chickens is significantly reduced. This property makes these viruses particularly suited for preparing a live vaccine for protecting poultry against disease conditions resulting from CAV infection.

Abstract: A multi-site in ovo injection apparatus and related methods for treating live eggs is disclosed. The multi-site injection apparatus includes one or more injection delivery devices which are configured to deliver multiple treatment substances to predetermined areas of eggs. The treatment substances can be provided so that they are spatially and/or temporally separate. The devices and methods enable the effective use of a plurality of treatment substances even those that are effective when used alone but can be noxious if mixed.

Abstract: A method of injecting a plurality of bird eggs comprises: (a) orienting a plurality of avian eggs in a predetermined position; (b) forming an opening in the shell of each of the eggs; (c) extending an elongate delivery device through each of the openings and into the eggs, each of the delivery devices comprising a detector and an injection needle, with the injection needle having a lumen formed therein; (d) detecting with the detector information from the interior of each of the plurality of eggs; and (e) injecting a substance into each of the plurality of eggs through the lumen of said injection needle. The detected information can be used for a variety of purposes, including adjusting the depth of penetration of the injection needle to more precisely control the location of the injection, identifying the gender of the eggs for subsequent sorting of the eggs, distinguishing viable from non-viable eggs, etc.

Abstract: An apparatus for injecting fluid substances into eggs is disclosed, comprising a plurality of injectors, needles disposed within each of the injectors moveable between a retracted position and an extended injecting position with respect to the injectors, and a bridge assembly for positioning the injectors in alignment with a corresponding plurality of eggs in an egg flat. The injectors rest substantially vertically in openings in a horizontal plate. The openings in the plate are slightly larger than the cross-section of the injectors for permitting the injectors to move vertically within the openings in the plate with respect to the plate. An egg nesting cup is pivotally secured to the lower end of each injector. The injectors are lowered to engage the eggs. When the plate and the injectors are lowered the nesting cups seat against the eggs and the injectors move vertically upward with respect to the plate.

Abstract: Disclosed is a process for growing the microflora Thraustochytrium, Schizochytrium, and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment of the present invention, the process produces microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further disclosed is a food product which includes Thraustochytrium, Schizochytrium, and mixtures thereof, and a component selected from flaxseed, rapeseed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids.