Abstract: An aerosol-generating amorphous solid comprising an active ingredient, wherein when a sheet of the aerosol-generating amorphous solid having an area of 43.1 mm2 and area density of 90 g/m2 is heated to 230° C. for a period of 3 seconds under a 1.1 L/min airflow, at least 50 wt % of the active ingredient is aerosolized.

Abstract: Disclosed herein is an aerosol generating substrate comprising an aerosol generating material. The aerosol generating material comprises an amorphous solid. The amorphous solid comprises an active ingredient, wherein at least 70 wt % of the active ingredient aerosolised on heating of the aerosol generating material to 370° C. for a ten-second period under a 1.95 L/min airflow.

Abstract: A method of producing a liquid tobacco extract is provided, the method including the steps of: preparing a tobacco starting material including natural tobacco material such that during the step of preparing the tobacco starting material, the tobacco is not subjected to any treatment adapted to alter the pH of the tobacco; heating the tobacco starting material in a flow of inert gas at an extraction temperature of between 125 degrees Celsius and 160 degrees Celsius for between 90 minutes and 270 minutes; collecting volatile compounds released from the natural tobacco starting material during the heating step; and forming a liquid tobacco extract comprising the collected volatile compounds.

Abstract: A method of preparing a whitened tobacco material for use in a smokeless tobacco product, including: (i) extracting a tobacco material with an aqueous solution to provide a tobacco solids material and a tobacco extract; (ii) bleaching the tobacco solids material with a solution comprising a strong base, an oxidizing agent, or a combination thereof to provide a bleached tobacco material; and (iii) drying the bleached tobacco material to provide the whitened tobacco material; wherein the tobacco solids material is not subjected to treatment at an elevated temperature with sulfur-containing reagents, organic solvents, sodium hydroxide, or an acid between the extraction and the bleaching.

Abstract: The disclosure provides a foam composition including a cellulosic foam stabilizer, a sweetener, optionally a natural gum, water, and a flavoring agent or an active ingredient or both a flavoring agent and an active ingredient. The disclosure also provides a method of forming a foam composition, including mixing an aqueous slurry of a cellulosic foam stabilizer with a sweetener, an optional natural gum, an optional alkali metal salt, and a flavoring agent or an active ingredient or both a flavoring agent and an active ingredient to form a mixture; introducing air into the mixture through stirring or bubbling to form a foam; dividing the foam into a plurality of discrete portions; and optionally drying the discrete portions of foam.

Abstract: The present disclosure provides a method of preparing whitened tobacco composition including chemically pulping a tobacco material to form a tobacco pulp, extracting the tobacco pulp with an extraction solution, treating the tobacco pulp with a solution comprising an oxidizing agent for a time and at a temperature sufficient to lighten the color of the tobacco pulp to give a whitened tobacco pulp, and drying the whitened tobacco pulp to provide the whitened tobacco composition. Alternative bleaching processes and treatment methods effective for preparing whitened tobacco compositions are also provided herein.

Abstract: An object of the present invention is to provide an F1 hybrid or a portion thereof, and use of the F1 hybrid or a portion thereof. The F1 hybrid of the present invention is an F1 hybrid between Nicotiana umbratica and Nicotiana kawakamii.

Abstract: Isolated O-methylated flavonoid compounds and mixtures of o-methylated flavonoid compounds derived from a plant of the Nicotiana species and methods of obtaining such compounds are provided. The o-methylated flavonoids can be combined with tobacco materials to produce tobacco products (e.g., smoking articles) to enhance the sensory characteristics thereof. The o-methyl flavonoids can alternatively be combined with non-tobacco materials for dietary supplement and/or pharmaceutical use.

Abstract: A method of preparing a whitened tobacco material for use in a smokeless tobacco product is provided, including: (i) extracting a tobacco material with an extraction solution to provide a tobacco solids material and a tobacco extract; (ii) cooking the tobacco solids material in an alkaline sulfite cooking liquor including sulfite ions and having a pH of greater than 7 to form a tobacco pulp; (iii) bleaching the tobacco pulp with a bleaching solution to provide a bleached tobacco material; and (iv) drying the bleached tobacco material to provide the whitened tobacco material.

Abstract: A method of manufacturing a flavor source includes: a step A of adding a basic substance to a tobacco raw material; a step B of heating the tobacco raw material added with the basic substance and releasing an inhaling flavor component into a vapor phase from the tobacco raw material; a step C of collecting the inhaling flavor component released into the vapor phase in the step B; a step D of washing a tobacco residue being the tobacco raw material obtained after the inhaling flavor component is released in the step B, with a washing solvent; a step E of pulverizing or cutting the tobacco residue obtained after being washed in the step D; and a step F of adding the inhaling flavor component collected in the step C to the tobacco residue obtained after pulverized or cut in the step E.

Abstract: A method for applying an ultraviolet curable coating material and a method for producing an ultraviolet cured film include the steps of: supplying an ultraviolet curable coating material containing an ultraviolet curable acrylic monomer into a mixer under a condition of greater than or equal to 8 MPa without diluting the ultraviolet curable coating material with an organic solvent; supplying carbon dioxide with a critical pressure or more into the mixer; mixing the ultraviolet curable coating material and the carbon dioxide supplied into the mixer to form a mixed fluid; spraying the mixed fluid under a condition of a critical pressure or more of the carbon dioxide to form a coating film; and irradiating the coating film with ultraviolet rays to form an ultraviolet cured film.

Abstract: An extraction method of a flavor constituent comprises: a step A for heating a tobacco raw material which is subjected to an alkali treatment; and a step B for bringing a release component released in the gas phase in the step A into contact with a collection solvent at normal temperature until any time from when a first condition is satisfied to when a second condition is satisfied. The first condition is determined based on a residual rate of nicotine component. The second condition is determined based on a remaining amount of nicotine component.

Abstract: A portable package including an inhaling flavor product including a flavor base material configured by a non-tobacco material, a tobacco raw material that has undergone an alkali treatment and emits an inhaling flavor component as a vapor phase, and a container portion that contains the tobacco raw material and the flavor base material, wherein the container portion limits a movement of at least one of the tobacco raw material and the flavor base material so as to maintain the tobacco raw material and the flavor base material in a non-contacting state, and the tobacco raw material and the flavor base material are arranged within a same space constructed by the container portion.

Abstract: A producing method of a tobacco raw material containing a flavor constituent, comprises: a step A for heating a tobacco raw material in a closed space which is treated with alkali and for taking a flavor constituent released in the gas phase from the tobacco raw material to an outside of the closed space; a step B for allowing a first solvent to capture the flavor constituent by bringing the flavor constituent released in the gas phase in the step A into contact with the first solvent which is a liquid substance at normal temperature on the outside of the closed space; a step C for supplying a second solvent to the tobacco raw material in the closed space after the step A and for taking a normal component which is released as the liquid phase from the tobacco raw material to the second solvent, together with the second solvent to the outside of the closed space; and after the steps B and C, the step D for adding the first solvent capturing the flavor constituent in the step B to the tobacco raw material from

Abstract: Methods for creating concentrated plant material solution, including combining ethanol-soluble, water-insoluble concentrated plant material with ethanol to define mixtures and reducing the amount of ethanol in the mixtures until the mixtures have viscosities compatible for use with electronic cigarettes. Some examples include combining ethanol-soluble, water-insoluble concentrated plant material, hydrocarbon bases, and liquid solutions containing ethanol to define mixtures, removing the hydrocarbon bases from the mixtures, and reducing the ethanol content of the mixtures until the mixtures define viscosities compatible for use with electronic cigarettes. Some examples include extracting solute from source materials and including the extracted solute in the ethanol-soluble, water-insoluble concentrated plant material.

Abstract: In one aspect, there is provided a mutant, non-naturally occurring or transgenic plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a member of the CLC family of chloride channels and having at least 60% sequence identity to SEQ ID NO:1 or SEQ ID NO:2 or SEQ ID NO:3 or SEQ ID NO:4 or SEQ ID NO:10 or SEQ ID NO:11; (ii) a polypeptide encoded by the polynucleotide set forth in (i); (iii) a polypeptide comprising, consisting or consisting essentially of a sequence encoding a member of the CLC family of chloride channels and having at least 60% sequence identity to SEQ ID NO:5 or SEQ ID NO:6 or SEQ ID NO:7 or SEQ ID NO:12 or SEQ ID NO:13 or SEQ ID NO:14; or (iv) a construct, vector or expression vector comprising the isolated polynucleotide set forth in (i); and wherein the expression or activity of the polynucleotide or the polypeptide is modulated as compared to a control plant and wherein the nitrate levels in the mutant, non-naturally occurring o

Abstract: Aspects of the present disclosure relate to electrochemical reduction of tobacco-specific nitrosamines (TSNAs). According to certain methods described herein, a tobacco composition containing one or more TSNAs and nicotine is contacted with a solvent to form a tobacco mixture. In some embodiments, the tobacco mixture is introduced into an electrochemical device comprising an anode and a cathode. The tobacco mixture may, in some cases, form at least part of an initial electrolyte mixture that is in physical contact with at least a portion of the anode and at least a portion of the cathode. In some instances, an electrical potential is applied between the anode and the cathode, thereby reducing one or more TSNAs in the initial electrolyte mixture and producing a reduced electrolyte mixture. In certain cases, application of the electrical potential between the anode and the cathode does not cause non-TSNA components of the tobacco mixture (e.g.

Abstract: A liquid tobacco composition comprises tobacco dissolved in an ionic liquid. Removal of selected tobacco constituents is aided by dissolving the tobacco in the ionic liquid. The tobacco, having selected constituents removed, may be regenerated from the liquid tobacco composition by separating the tobacco from the ionic liquid.

Abstract: Provided herein are bacterial strains that are capable of degrading nitrite and/or TSNAs. Also provided herein are methods of using such bacterial strains to degrade nitrite and/or TSNAs.

Abstract: The present invention relates to a method for producing articles impregnated with at least one plant substance from at least one plant, characterized in that it comprises the following steps: a) Extraction and/or pressing of at least one plant (V1), or at least one part of said plant, producing a liquid plant extract (E1)) and a solid fibrous residue (R1), then b) Separation of said plant extract (E1) from said fibrous residue (R1), and c) Destructuring of said fibrous residue (R1), d) Production of a fibrous web or of an article made from the fibrous residue (R1) obtained in step c), and e) Impregnation of said fibrous residue (R1) with (i) at least said plant extract (E1), which is optionally concentrated, purified, flavored and/or fragranced, with (ii) at least one water-soluble or liposoluble plant substance isolated from said plant extract (E1), with (iii) at least one composition comprising at least one optionally concentrated, purified, flavored and/or fragranced water-soluble or liposoluble substan

Abstract: Disclosed are a process and an apparatus for improving tobacco raw material. The process mainly comprises performing an extraction from at least one of tobacco leafs, tobacco flakes, tobacco powder, stalks and cut stems by mixed solvents, conducting a solid-liquid separation for the tobacco raw material, applying the extract liquor of tobacco leaves, flakes and cuts for manufacturing tobacco extract and that of stalks and cut stems for manufacturing fertilizer, diluting and mixing a given amount of tobacco aqueous extract and ethanol extract and removing harmful components so as to get feed liquid, backfilling the feed liquid to the tobacco raw material, and drying the tobacco raw material to a certain dryness to obtain the products. The apparatus is a system composed of a plurality of interconnected independent devices such as extractor, solid-liquid separator, a feed liquid backfiller, etc.

Abstract: Methods for creating concentrated plant material solution, including combining ethanol-soluble, water-insoluble concentrated plant material with ethanol to define mixtures and reducing the amount of ethanol in the mixtures until the mixtures have viscosities compatible for use with electronic cigarettes. Some examples include combining ethanol-soluble, water-insoluble concentrated plant material, hydrocarbon bases, and liquid solutions containing ethanol to define mixtures, removing the hydrocarbon bases from the mixtures, and reducing the ethanol content of the mixtures until the mixtures define viscosities compatible for use with electronic cigarettes. Some examples include extracting solute from source materials and including the extracted solute in the ethanol-soluble, water-insoluble concentrated plant material.

Abstract: Methods of modifying the tobacco-specific nitrosamine content of a tobacco material are described herein. One exemplary method comprises contacting a tobacco material with a composition comprising salt, sugar, enzyme, lactic acid bacteria, yeast, or a combination thereof to reduce the total bacterial content; curing the tobacco material; and fermenting the tobacco material in the presence of one or more microorganisms. The method can provide a fermented tobacco material having a tobacco-specific nitrosamine content that is reduced relative to a fermented tobacco material that has not been subjected to the disclosed method steps. In certain embodiments, the tobacco-specific nitrosamine content of the fermented tobacco material is no more than that of the cured tobacco material. Tobacco-containing products including such treated tobacco materials are also provided.

Abstract: The invention provides a smokeless tobacco product comprising a tobacco extract. In some embodiments, the smokeless tobacco product is translucent. The invention further provides methods for making and using the smokeless tobacco product. In certain embodiments, the smokeless tobacco product comprises isomalt, maltitol syrup, and a translucent tobacco extract prepared by ultrafiltration.

Abstract: Tobacco smoking mixtures capable of producing tobacco smoke are provided which reduce the cytotoxicity and/or mutagenicity of the tobacco smoke. The tobacco smoking mixtures include tobacco cut filler and glycerin in an amount effective to reduce cytotoxicity and/or mutagenicity of smoke produced by the tobacco smoking mixture. The tobacco smoking mixtures enable a further reduction in cytotoxicity and/or mutagenicity when glycerin is combined with palladium or salts of calcium and/or magnesium.

Abstract: A class of molecularly imprinted polymers that specifically recognizes and binds to nitrosamines members of which class are useful, for example, in analysis and separation of nitrosamines from biological fluids. Such molecularly imprinted polymers are also useful in methods of treating and manufacturing tobacco products and materials.

Abstract: The present application is related to an electronic cigarette liquid and a method of producing pulp for use in the electronic cigarette liquid, the method includes: using a stainless steel bathing can with a 500 Liter capacity that can endure heating and pressurizing; placing the pulp and original liquid used for bathing inside the can at a pre-determined ratio. After heating, pressurizing and bathing, the absorption of the pulp is fully attained. Then, opening a ball valve and releasing and collecting only the liquid from the bathing can and taking out the viscous pulp for packaging. The hookah pulp product is obtained with a solid extract of the hookah pulp product being 90% or above of the hookah pulp product.

Abstract: The present patent application provides an oral nicotine-substituted cytisine atomized liquid and its preparation method. The oral nicotine-substituted cytisine atomized liquid includes the following components in mass percentage per 1 L of the oral atomized liquid: 0.1-10% of tobacco, 0.3-15% of cocoa extract, 0.1-0.9% of cytisine, 0.1-0.5% of TWEEN 80 and 75-90% of primer. The liquid can be obtained through the steps of weighing tobacco into an extraction tank, filtering the extract to obtain the filtrate, adding cocoa extract, cytisine and primer to the filtrate and mixing evenly.

Abstract: A method of preparing a whitened tobacco material is provided, the method including the steps of (i) extracting a tobacco material with an aqueous solution to give a tobacco pulp and a tobacco extract; (ii) treating the tobacco pulp with at least one of a caustic reagent and an oxidizing agent for a time and at a temperature sufficient to lighten the color of the tobacco pulp to give a whitened tobacco pulp; (iii) clarifying the tobacco extract to remove higher molecular weight components; and (iv) combining the whitened tobacco pulp with a clarified tobacco extract to form a whitened tobacco material. The whitened tobacco material can be isolated and incorporated into a smokeless tobacco product. The invention also provides a smokeless tobacco product incorporating a whitened tobacco material. The smokeless tobacco product may be a snus-type formulation contained within a sealed pouch.

Abstract: In one aspect, there is provided a method for reducing the formation of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and N-nitrosonornicotine and N-nitrosoanatabine and N-nitrosoanabasine in a tobacco homogenate comprising the steps of: (a) providing a tobacco homogenate; (b) increasing the pH of the tobacco homogenate to at least about pH 6.0; (c) optionally measuring the concentration of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and N-nitrosonornicotine and N-nitrosoanatabine and N-nitrosoanabasine in the tobacco homogenate before and after the pH treatment in step (b); and (d) obtaining a tobacco homogenate in which the levels of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and N-nitrosonornicotine and N-nitrosoanatabine and N-nitrosoanabasine are reduced as compared to the levels of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and N-nitrosonornicotine and N-nitrosoanatabine and N-nitrosoanabasine in the tobacco homogenate provided in step (a).

Abstract: A method for producing lutein from one or more plants of genus Nicotiana is provided. The lutein can be derived inter alia from Nicotiana species biomass. A method such as is described in various embodiments herein also provides articles and compositions that include lutein produced from one or more plants of genus Nicotiana.

Abstract: A class of molecularly imprinted polymers that specifically recognizes and binds to TSNAs are useful, for example, in analysis and separation of TSNAs from biological fluids. Such polymers are also useful in methods of treating and manufacturing tobacco products and materials. Also disclosed are selectively adsorbent non-molecularly imprinted polymer of a non-acidic monomer and a cross-linker having polar functionality, one of which is hydrophilic, the other of which is hydrophobic, and the use of such polymers in the treatment of materials containing nicotine and nitrosamine.

Abstract: Porous beads are provided of a polymer of a non-acidic monomer and a cross-linker having polar functionality, one of which is hydrophilic, the other of which is hydrophobic, further comprising residues of polyvinyl alcohol. The beads may be molecularly imprinted or non-molecularly imprinted. The use of such beads in the removal of nitroso-containing compounds from material containing them is also disclosed. Also disclosed is a non-molecularly imprinted polymer which is selectively adsorbent for at least one tobacco specific nitrosamine in the presence of nicotine, said polymer being a polymerization product of a non-acidic monomer and a cross-linker having polar functionality, one of which is hydrophilic, the other of which is hydrophobic.

Abstract: The invention provides a method of isolating certain target compounds from tobacco, tobacco materials or smoke generated by a smoking article. The method can be used to remove undesirable compounds from tobacco, tobacco materials, or tobacco smoke. The method can also be used to remove flavor compounds from tobacco or tobacco materials, which can then be used as flavor components for tobacco material used in smoking articles and smokeless tobacco compositions.

Abstract: Methods for the selective removal of specific constituents of tobacco extract include contacting an extract with a nitrosamine selective adsorption agent, a metal selective adsorption agent and/or a nitrate selective adsorption agent. Preferred characteristics of such agents are identified.

Abstract: A method for extracting and isolating constituents of cellulosic matter, comprising providing cellulosic matter; contacting the cellulosic matter with a supercritical fluid; separating the supercritical fluid from the cellulosic matter, thereby forming an extract and a raffinate; and separating at least one compound present in the extraction medium by distillation.

Abstract: The invention relates to methods of nicotine extraction from tobacco, caporal and tobacco crumb. The proposed method for extraction of nicotine from tobacco, caporal and tobacco crumb implies continuous extraction from raw material with low-boiling solvents at vapor phase followed by solvent stripper and recurrence for further reuse in the process.

Abstract: A method of thermally processing a tobacco material is provided, the method including the steps of (i) mixing a tobacco material, water, and an additive selected from the group consisting of lysine, glycine, histidine, alanine, methionine, glutamic acid, aspartic acid, proline, phenylalanine, valine, arginine, di- and trivalent cations, asparaginase, saccharides, phenolic compounds, reducing agents, compounds having a free thiol group, oxidizing agents, oxidation catalysts, plant extracts, and combinations thereof, to form a moist tobacco mixture; (ii) heating the moist tobacco mixture at a temperature of at least about 60° C. to form a heat-treated tobacco mixture; and (iii) incorporating the heat-treated tobacco mixture into a tobacco product.

Abstract: The invention provides a tobacco composition for use in a smoking article or a smokeless tobacco composition that includes an additive derived from a flower of the Nicotiana species. The additive can be a flower of the Nicotiana species or a portion thereof in particulate form or in the form of flower isolate derived from a flower of the Nicotiana species. In certain embodiments, the flower isolate is in the form of an extract from a flower of the Nicotiana species or in the form of a chemically transformed flower isolate, the chemical transformation being selected from acid/base reaction, hydrolysis, thermal treatment, and enzymatic treatment. The invention also provides smoking articles and smokeless tobacco compositions that include the flower additives described herein, and methods for preparing an additive derived from a flower of the Nicotiana species for addition to a tobacco composition.

Abstract: The present patent application provides an oral nicotine-substituted cytisine atomized liquid and its preparation method. The oral nicotine-substituted cytisine atomized liquid includes the following components in mass percentage per 1 L of the oral atomized liquid: 0.1-10% of tobacco, 0.3-15% of cocoa extract, 0.1-0.9% of cytisine, 0.1-0.5% of TWEEN 80 and 75-90% of primer. The liquid can be obtained through the steps of weighing tobacco into an extraction tank, filtering the extract to obtain the filtrate, adding cocoa extract, cytisine and primer to the filtrate and mixing evenly.

Abstract: A method of forming a liquor comprising tobacco solutes, the method comprising extracting tobacco solutes from tobacco by flowing an extraction solvent through tobacco to form a tobacco solutes-rich extraction solvent and a tobacco solutes-poor tobacco, and forming tobacco solutes-rich liquor by flowing the tobacco solutes-rich extraction solvent through an entrapment solvent, wherein the tobacco solutes comprise nicotine and at least one tobacco flavor compound and/or tobacco aroma compound and the liquor comprises the tobacco solutes dissolved in the entrapment solvent. A preferred extraction solvent comprises a supercritical fluid. Also provided are cigarettes and cigarette components comprising extracted tobacco solutes such as flavor compounds, aroma compounds and nicotine. Further, tobacco from which aroma compounds and/or nicotine have been extracted can be used in cigarettes.

Abstract: A class of molecularly imprinted polymers that specifically recognizes and binds to nitrosamines members of which class are useful, for example, in analysis and separation of nitrosamines from biological fluids. Such molecularly imprinted polymers are also useful in methods of treating and manufacturing tobacco products and materials.

Abstract: A cigarillo includes a tobacco rod including a tobacco cut filler circumscribed by an inner binder, a filter adjacent to the tobacco rod, a band of tipping paper circumscribing the filter, and an outer wrapper of natural tobacco circumscribing the tobacco rod and a rod end portion of the band of tipping paper such that a mouth end portion of the band of tipping paper is visible. A method of making the cigarillo includes convoluting an inner binder around a tobacco cut filler to form a tobacco rod, axially aligning and abutting a filter with the tobacco rod, convoluting a band of tipping paper around the filter, and convoluting an outer wrapper of natural tobacco around the tobacco rod and a rod end portion of the band of tipping paper such that a mouth end portion of the tipping paper is visible.

Abstract: Method of manufacturing a cigarette having equivalent aroma and flavor even with a reduced amount of burley leaves. The method does not require special processing treatment due to the absence of necessity of an installation space and costs for the treatment. Also, method of manufacturing a cigarette containing the same, and a cigarette. The invention separates raw material for sheet tobacco into fiber and solution, adds a primary casing flavor to the solution, mixes the solution added with the primary casing flavor and the fiber to produce a mixture, and dries the mixture. Burley leaf tobacco is thereafter mixed with the sheet tobacco. The casing flavor is previously added at the time of the molding of sheet tobacco in the sheet-tobacco manufacturing process, which makes enables production of sheet tobacco having the aroma and flavor equivalent of when burley leaf tobacco is used, even without special processing treatment.

Abstract: A method for reducing the tobacco specific nitrosamines (TSNAs) in tobacco and tobacco smoke is provided. The tobacco is combined with a solvent to form a soluble extracts fraction and an insoluble residue fraction. The soluble extracts fraction is contacted with a nitrosamine-reducing material to provide a soluble extracts fraction having a reduced level of TSNAs. The nitrosamine-reducing material may comprise a sepiolite. The insoluble residue fraction is contacted with an alkali extractant to provide an insoluble residue fraction having a reduced level of TSNAs. The alkali extractant may comprise a hydroxide. The soluble extracts fraction having a reduced level of TSNAs and insoluble residue fraction having a reduced level of TSNAs may be recombined to form a reconstituted tobacco product having a reduced level of TSNAs. The TSNAs may be reduced by at least about 30%, such as at least about 40%, such as at least about 50%.

Abstract: The present disclosure describes methods of obtaining and/or deriving a sugar-enriched extract from plants of the Nicotiana species and methods for incorporation of such extracts into various tobacco compositions. One exemplary method for preparing a sugar-enriched extract comprises contacting Nicotiana plant material with a solvent and extracting sugars into the solvent to form a liquid sugar-containing extract; separating a solid extracted plant material from the liquid sugar-containing extract; clarifying the liquid sugar-containing extract to form a clarified sugar-containing extract and a solids fraction; and isolating the liquid sugar-containing extract to provide a sugar-enriched extract comprising a combined weight of fructose and glucose of at least about 10% by dry weight. In certain embodiments, the sugars of the extract comprise at least about 50% by weight fructose.

Abstract: The present disclosure describes methods of obtaining and/or deriving proteins from plants of the Nicotiana species and methods for incorporation of such proteins into various products. For example, a method for obtaining a protein-enriched material from a plant of the Nicotiana species or portion thereof is provided, comprising: extracting one or more proteins from the plant material into a solvent to form a liquid protein-containing extract; separating a solid extracted plant material from the liquid protein-containing extract; clarifying the liquid protein-containing extract to form a clarified protein-containing extract and a solids fraction; and treating the clarified protein-containing extract so as to provide a protein-enriched material comprising at least about 60% protein by weight.

Abstract: Provided herein are bacterial strains that are capable of degrading nitrite and/or TSNAs. Also provided herein are methods of using such bacterial strains to degrade nitrite and/or TSNAs.

Abstract: A kit is disclosed for supplying a cartridge of an inhaler article with liquid tobacco extract, the kit comprising an apparatus having means for extracting a liquid tobacco extract from a tobacco derived solid. The apparatus is adapted to deliver liquid tobacco extract to a cartridge and a cartridge adapted to receive liquid tobacco extract from the apparatus. An apparatus for supplying a liquid tobacco extract to a cartridge of an inhaler article is further disclosed. The apparatus has a housing, means for extracting a liquid tobacco extract from a tobacco derived solid and said housing is adapted to deliver liquid tobacco extract to a cartridge of an inhaler article. A related method of supplying a cartridge of an inhaler article with a liquid tobacco extract is further disclosed.

Abstract: The present application is related to an electronic cigarette liquid and a method of producing pulp for use in the electronic cigarette liquid, the method includes: using a stainless steel bathing can with a 500 Liter capacity that can endure heating and pressurizing; placing the pulp and original liquid used for bathing inside the can at a pre-determined ratio. After heating, pressurizing and bathing, the absorption of the pulp is fully attained. Then, opening a ball valve and releasing and collecting only the liquid from the bathing can and taking out the viscous pulp for packaging. The hookah pulp product is obtained with a solid extract of the hookah pulp product being 90% or above of the hookah pulp product.