Abstract: A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system. An infusion pump for delivering a fluid from a fluid source may be integrated in a microfluidic chip using a capping module having pumping components formed therein.

Abstract: A composition including the fermentation supernatant from a fermentation of yeast is intended to be conveniently introduced through the wastewater plumbing system of a private home or other facility into a septic system servicing the home or other facility to substantially accelerate the ability of the bacteria resident in the septic system to substantially digest biologically available organic compounds present in the septic system, and methods of accomplishing the same.

Abstract: The present invention relates to a method of dewatering whole stillage comprising subjecting whole stillage to one or more enzymes capable of degrading a whole stillage component and separating the material obtained in step i) into a solid fraction and a liquid fraction.

Abstract: The present invention relates to enhancing sludge dewaterability by adding an alpha-amylase to the sludge prior to conventional conditioning and dewatering operations.

Abstract: Surfactants and flow through capacitors (3) work together synergistically to enhance each other's function. Surfactants added upstream into the feed water (1) enhance the flow through capacitor's (3) ability to purify organic compounds from fluid streams. Surfactants (13) added downstream into the product water enhance the micelle forming processes so that they work more effectively in appliances (11), by working in smaller amounts or concentrations, and with less tendency to precipitate, form a solid or a crud.

Abstract: The invention relates to methods and compositions for of treating water systems, particularly recirculating water systems contaminated by a biofilm containing sessile micro-organisms. The method includes the steps of forming a boron conditioned enzyme and contacting the biofilm with said boron conditioned enzyme, thereby planktonising the micro-organisms The boron conditioned enzyme retains a level of activity at least 40% of the initial activity of the unconditioned enzyme for at least two hours after contacting the biofilm, either in the presence or absence of a biocide or corrosion inhibitor which would normally deactivate the enzyme. In preferred embodiments a biocide is added with the conditioned enzyme. The method and compositions are also applicable to remediation of tepid water systems in which biofilm may harbour pathogens such as sessile Legionella.

Abstract: A method of managing organic waste and an organic waste management system. The method includes the steps of receiving organic waste liquids and solids in a collection vessel, such as a grease trap. The dissolved oxygen is monitored, the pH level is monitored, the flow is monitored, and the temperate of the organic waste liquids and solids in the collection vessel are all monitored and data is generated therefrom. Enzymes and bacteria are generated/supplied and delivered along with other reagents to the collection vessel in response to the data. In addition, dissolved oxygen, air, or equivalent is applied to the collection vessel in response to the data so that the organic waste liquids and solids are degraded.

Abstract: A solid formulation for use with sodium chloride and a chlorine generator to provide safe, clean chlorinated recreational water. The formulation comprises a chlorine stabilizing agent and a phosphate remover agent. An enhanced salt comprising a chlorine stabilizing agent, a phosphate remover agent and sodium chloride is also described. The formulation and enhanced salt can also contain a metal chelating agent and a pH balancing agent. A cleaning agent may also be included. A fast dissolving sodium cyanurate compound is used for the chlorine stabilizing agent. A method, kit, and system for providing safe, clean chlorinated recreational water are also disclosed.

Abstract: A method of treating wastewater sources such as sewer systems or those from pulp and paper mills which contain biomass waste involves adding white rot fungi in the presence of glucose. While glucose may be directly added to the wastewater source, it has been found to be more economical to reduce or substantially remove color from the wastewater by additionally adding brown rot fungi in the presence of cellulose and hemi-cellulose. The addition of bacteria has also been found helpful to improve the reduction in color, biological oxygen demand, as well as chemical oxygen demand in wastewater sludge reduction.

Abstract: The present invention relates to a process for treating a wastewater comprising an ammonium ion species and a phosphorus ion species. The process comprises the steps of: (i) treating the effluent in a first stage of the process to convert the phosphorus to a phosphorus-containing salt; (ii) treating the wastewater in a second stage of the process to convert the ammonium ion species to gaseous ammonia; (iii) removing the struvite and/or other phosphorous containing salts from the effluent; and (iv) removing the gaseous ammonia from the wastewater.

Abstract: A process and system for treating excess sludge produced from a biological treatment system. The process includes biologically treating water or wastewater and producing excess sludge. The excess sludge is directed to a solubilizer where the sludge is solubilized. The solubilized sludge is directed to a liquid/solid separator, which separates the solubilized sludge into a liquid fraction and a solid fraction. The process further includes at least partially digesting the liquid fraction and directing the partially digested liquid fraction to the biological treatment system where the liquid fraction is subjected to further treatment. Finally, the solid fraction is subject to further solubilization.

Abstract: A system for composting-free disposal of organic waste and method thereof included a heating apparatus, a pretreatment apparatus, and a decomposition apparatus. The heating apparatus heats the pretreatment apparatus and the decomposition apparatus. The organic waste is separated into a slurry and a surface oil-water mixed liquid by the pretreatment apparatus. Microbial enzymes and raw material are added into the slurry to become a mixture. The mixture is then decomposed and sterilized by the decomposition apparatus to become a composting-free organic fertilizer product. All these procedures can be carried out within 3 to 24 hours. The system and the method thereof provides an efficiently fast, space-saving way to deal with organic waste and achieve environmental protection and sanitation.

Abstract: The invention relates to a method for improving the performance of filtration devices by adding P- and/or N-containing compounds to the medium to be filtered.

Abstract: A method of treating wastewater sources such as those from pulp and paper mills which contain lignin involves adding white rot fungi in the presence of glucose. While glucose may be directly added to the wastewater source, it has been found to be more economical to reduce or substantially remove color from the wastewater by additionally adding brown rot fungi in the presence of cellulose and hemi-cellulose. The addition of bacteria has also been found helpful to improve the reduction in color, biological oxygen demand, as well as chemical oxygen demand in wastewater.

Abstract: It is an object to provide an organic waste processing method and apparatus capable of making a formalin nontoxic and decreasing an amount and/or a volume of a formalin-fixed organic waste easily in a shorter time without retaining an original form. A formalin-fixed organic waste processing method comprising the steps of formalin neutralizing a formalin-fixed organic waste, decomposing the waste using an enzyme, and subsequently, carrying out a drying process, and an organic waste processing apparatus comprising a processing chamber 2 for processing the waste, a stirring paddle provided in the processing chamber 2, a driving motor of the paddle, a heater 5 for the processing chamber 2, air supply means 6, exhaust means 14, and control means 7 for controlling the operations of the driving motor, the heater 5, the air supply means 6 and the exhaust means 14.

Abstract: A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system. The microfluidic element may comprise a matrix having an affinity for selected molecules in a sample. The matrix binds, reacts with and/or retains the selected molecules without affecting other molecules in the sample.

Abstract: Systems and methods for reducing biofilms are described. The systems and methods are particularly suitable for use with conventional aqueous crossflow filtration systems, such as reverse osmosis systems. The addition of the enzyme/surfactant compound has been found to enhance the effectiveness of conventional crossflow filtration processes by decreasing or inhibiting the growth of biofilms and other contaminants.

Abstract: A variety of pollutants and other contaminants may be removed from a variety of aqueous media using electrolytic treatments. The treatment includes inserting an anode and a cathode into the medium undergoing treatment, and applying a high current and voltage to the electrodes. The treatment may also include the addition of catalytic enzymes to the medium undergoing treatment. The present methods are compatible with aquatic lifeforms, and offer an effective alternative to chemical-based water treatment systems.

Abstract: The present invention relates to methods for preventing or removing biofilm on a surface, comprising contacting the surface with an effective amount of a composition comprising one or more acylases and a carrier to degrade a lactone produced by one or more microorganisms, wherein the degradation of the lactone prevents or removes the biofilm.

Abstract: A method of antioxidation and antioxidant-functioning water that can transform an antioxidation subject that is in an oxidation state due to a deficiency of electrons, or for which protection from oxidation is desired, into a reduced state where electrons are satisfied, by promoting the breaking reaction of molecular hydrogen that is used as a substrate included in hydrogen-dissolved water into a product of active hydrogen through a process employing a catalyst on the hydrogen-dissolved water, while anticipating high benchmarks of safety on the human body and reduced environmental burden.

Abstract: A method for extracting contaminants and pollutants from an ecosystem. The method includes extracting from the ecosystem a quantity of animal tissue and grinding or macerating the animal tissue to form a slurry-like mixture and adding the mixture to a digestion vessel. The pH of the ground animal tissue is reduced to a range of from approximately 3.5 to approximately 4.5 and the ground tissue is allowed to decompose through the action of digestive enzymes released from the visceral tissue of the animal tissue by the maceration step. The decomposed tissue is separated into a lipid portion, a proteinaceous portion and a bone fragment or hard tissue portion. At least a part of the lipid portion may then be extracted from the decomposed tissue for further processing or disposal of contaminants and pollutants contained therein.

Abstract: A method of dewatering sludge comprising adding an effective amount of one or more cellulolytic enzymes, one or more oxidants and one or more flocculants to the sludge to form a mixture of water and coagulated and flocculated solids and separating the coagulated and flocculated solids from the water.

Abstract: This invention concerns a method of dewatering sludge comprising adding an effective amount of one or more cellulolytic enzymes and one or more peroxidases to the sludge.

Abstract: Systems and methods for reducing biofilms are described. The systems and methods are particularly suitable for use with conventional aqueous crossflow filtration systems, such as reverse osmosis systems. The addition of the enzyme/surfactant compound has been found to enhance the effectiveness of conventional crossflow filtration processes by decreasing or inhibiting the growth of biofilms and other contaminants.

Abstract: A device for performing a biological modification of a fluid, the device includes (a) a chamber having an inlet for intake of the fluid and an outlet for outflow of the fluid; and (b) a collection of micro-organ cultures of at least one organ for performing the biological modification of the fluid, each individual micro-organ culture of the collection including cells and having dimensions, such that cells positioned deepest within the individual micro-organ culture are at least about 100 micrometers and not more than about 225 micrometers away from a nearest surface of the individual micro-organ culture, thereby in vivo organ architecture (organ structure) of organ units (e.g., acinus of liver) is maintained within each individual micro-organ culture, the collection of micro-organ cultures being located within the chamber and the collection of micro-organ cultures being in contact with at least a portion of the fluid flowing through the chamber.

Abstract: A process to recover energy, reduce pollution potential, and add value to organic waste such as animal manure is described. The process involves the anaerobic digestion of feedstocks, such as animal manure, at low to high temperatures in batch, semi-continuous or continuous reactors. The process makes use of existing handling and storage equipment at the farm and requires minimal supervision and skill by the operator. The system is not affected by high concentrations of volatile acids and ammonia or nitrogen. The productivity of the anaerobic digester system, in terms of methane gas production and quality, is exceptionally high. The anaerobic digester employs plural pressurizable anaerobic digesters connected in parallel. Consequently, the process is scaleable, low cost and does not interfere with regular farm operations.

Abstract: A method for treating potato wastewater comprising the steps of pretreating a potato wastewater stream including suspended and dissolved solids with an alpha amylase enzyme to hydrolyze starch in the wastewater, ultra-filtering the enzyme-treated wastewater from step (a) to filter and remove a portion of the suspended solids in a first concentrate stream separated from the wastewater, and reverse osmosis-filtering the ultra-filtered wastewater from step (b) to remove at least about 70% of the wastewater dissolved and suspended solids in a second concentrate stream.

Abstract: The invention relates to the elucidation of the breakdown of foreign substance in the metabolism of a liquid, chemical or biological reaction system by the analytical determination of the breakdown products (metabolites) produced. It is common practice for this elucidation to be carried out “blind”, i.e. without systematic prediction of the breakdown substances, by comparison and subtraction of analytical data sets, which are obtained by, for example, separating the substances in the liquid using liquid chromatography and measuring them using mass spectrometry before and after the foreign substance has been added.

Abstract: Systems and methods for reducing biofilms are described. The systems and methods are particularly suitable for use with conventional aqueous crossflow filtration systems, such as reverse osmosis systems. The addition of the enzyme/surfactant compound has been found to enhance the effectiveness of conventional crossflow filtration processes by decreasing or inhibiting the growth of biofilms and other contaminants.

Abstract: A method for separating a blood component, e.g., fibrin monomer, from blood or plasma. The method utilizes a container with a reaction chamber for receiving the plasma. The reaction chamber is defined by a wall and an agent is supplied to the reaction chamber for converting the fibrinogen content of the plasma into a non-cross-linked fibrin polymer. The method also utilizes a device for centrifuging the reaction chamber with the plasma and the agent to a degree sufficient for separating the non-cross-linked fibrin polymer from the plasma, for depositing the polymer on the outer wall of the reaction chamber, and for expelling the remaining plasma from the reaction chamber. A solvent is supplied to the reaction chamber for dissolving the non-cross-linked fibrin polymer. The solvent is supplied to the reaction chamber in a first amount for dissolving the non-cross-linked fibrin polymer which is less than the total amount of solvent for dissolving the polymer.

Abstract: A treatment unit is disclosed for treating a fluid in continuous mode. This treatment unit provides the opportunity to carry out simultaneously an enzymatic transformation and a capture of a fluid element. The unit has a reservoir with a fluid inlet for receiving a fluid to be treated and a fluid outlet for releasing a treated fluid. The unit also has removable cassettes provided with a reactive material for treating the fluid and two spaced-apart baffle walls in the reservoir for regulating the flow of the fluid therein. The unit further has a reaction chamber defined between each of the two spaced-apart baffles walls. The reaction chamber has an opening for removably inserting therein the cassette. Yet, the unit further has mounting means for mounting the cassette in a reaction chamber spaced-apart from the two baffle walls, whereby a cassette is disposed between two spaced-apart baffle walls and causes the fluid to flow in a zigzag pattern thus further regulating the flow of the fluid.

Abstract: A device for performing a biological modification of a fluid, the device includes (a) a chamber having an inlet for intake of the fluid and an outlet for outflow of the fluid; and (b) a collection of micro-organ cultures of at least one organ for performing the biological modification of the fluid, each individual micro-organ culture of the collection including cells and having dimensions, such that cells positioned deepest within the individual micro-organ culture are at least about 100 micrometers and not more than about 225 micrometers away from a nearest surface of the individual micro-organ culture, thereby in vivo organ architecture (organ structure) of organ units (e.g., acinus of liver) is maintained within each individual micro-organ culture, the collection of micro-organ cultures being located within the chamber and the collection of micro-organ cultures being in contact with at least a portion of the fluid flowing through the chamber.

Abstract: The present invention relates to a method for purifying contaminated water coming from a carwash and an installation for performing this method. The method comprises the steps of pretreating the contaminated water such that the size of the organic material it contains is smaller than a predetermined cut off value; subsequently carrying the contaminated water in upward direction through a packed bed of particulate material, in which micro-organisms produce exo-enzymes to degrade the organic material in the contaminated water, which bed is aerated from below, and draining the purified water from the packed bed at a level located a determined distance from the top of the bed; wherein an additive is added to the contaminated water to stimulate production of exo-enzymes by the micro-organisms in the packed bed.

Abstract: A method for treating potato wastewater comprising the steps of pretreating a potato wastewater stream including suspended and dissolved solids with an alpha amylase enzyme to hydrolyze starch in the wastewater, ultra-filtering the enzyme-treated wastewater from step (a) to filter and remove a portion of the suspended solids in a first concentrate stream separated from the wastewater, and reverse osmosis-filtering the ultra-filtered wastewater from step (b) to remove at least about 70% of the wastewater dissolved and suspended solids in a second concentrate stream.

Abstract: A treatment unit is disclosed for treating a fluid in continuous. This treatment unit provides the opportunity to carry out simultaneously an enzymatic transformation and a capture of a fluid element. The unit has a reservoir with a fluid inlet for receiving a fluid to be treated and a fluid outlet for releasing a treated fluid. The unit also has removable cassettes provided with a reactive material for treating the fluid and two spaced-apart baffle walls in the reservoir for regulating the flow of the fluid therein. The unit further has a reaction chamber defined between each of the two spaced-apart baffles walls. The reaction chamber has an opening for removably inserting therein the cassette. Yet, the unit further has mounting means for mounting the cassette in a reaction chamber spaced-apart from the two baffle walls, whereby a cassette is disposed between two spaced-apart baffle walls and causes the fluid to flow in a zigzag pattern thus further regulating the flow of the fluid.

Abstract: A partially soluble lanthanide compound and methods for use in removing phosphate from water. Preferably the compound is used in removing phosphate from water in swimming pools, spas, and similar structures. Alternatively, a combination of compounds having varying solubilities may be used to remove phosphate from water. Several water treatment techniques are disclosed, as well as a variety of different methods for delivery of the active ingredients. These delivery methods include use of a slurry of the active reagent in solution as well as a tablet, powder, or granulated structure. Additionally, the water treatment techniques may incorporate the use of a combination including both enzymatic compositions and compounds for phosphate removal.

Abstract: A partially soluble lanthanide compound and methods for use in removing phosphate from water. Preferably the compound is used in removing phosphate from water in swimming pools, spas, and similar structures. Alternatively, a combination of compounds having varying solubilities may be used to remove phosphate from water. Several water treatment techniques are disclosed, as well as a variety of different methods for delivery of the active ingredients. These delivery methods include use of a slurry of the active reagent in solution as well as a tablet, powder, or granulated structure. Additionally, the water treatment techniques may incorporate the use of a combination including both enzymatic compositions and compounds for phosphate removal.

Abstract: Sewage waste digestive bacteria and enzymes are incorporated into a slow release material and delivered to the site of the waste to digest the solid waste. The slow release material is made heavy so the enzymes and bacteria will be delivered to the sludge in the bottom of a sewage digester chamber and made fat soluble so that the enzymes and bacteria will be delivered to the grease in the waste system to be digested. This selective delivery system prevents the enzymes and bacteria from being diluted in grey water rendering them less effective and discharged from the sewage system without digesting waste.

Abstract: A process to recover energy, reduce pollution potential, and add value to organic waste such as animal manure is described. The process involves the anaerobic digestion of feedstocks, such as animal manure, at low to high temperatures in batch, semi-continuous or continuous reactors. The process makes use of existing handling and storage equipment at the farm and requires minimal supervision and skill by the operator. The system is not affected by high concentrations of volatile acids and ammonia or nitrogen. The productivity of the anaerobic digester system, in terms of methane a gas production and quality, is exceptionally high. The anaerobic digester requires only a singe reaction vessel. Consequently, the process is low cost and does not interfere with regular farm operations.

Abstract: A method and arrangement for sewage pre-treatment in a sewer pipeline infrastructure upstream of a sewage treatment facility of a sewage treatment system includes selecting a site in the sewer pipeline infrastructure between a source of untreated sewage and the sewage treatment facility of the system, providing a source of a biological activity enhancing agent at the preselected site, and introducing the agent from the source thereof into a flow of untreated sewage at the selected site so as to convert the sewage from an untreated state to a pre-treated state in the pipeline infrastructure upstream of the sewage treatment facility.

Abstract: Affinity macroligands (AML) comprising a reversibly water soluble poly-N-alkylacrylamide linked to a ligand, and their use in the separation of a target substance from a mixture comprising the target substance and other materials are disclosed. Preferred AMLs have number average molar masses of less than 7,000 g/mol (chain length n typically about 25), mass distributions of less than 1.2 and narrow precipitation-solubility temperature (NPST) interval, typically in the range of 1-2° C.

Abstract: A process for manufacturing fiber boards and similar wood-based products from a lignocellulosic raw material having a moisture content of at least 10 wt-%. According to the process, an aqueous fraction is separated from the fibers of the raw material, which fraction later is recycled to the process in order to form at least a part of the agent used for binding the fibers. Before mixing with the fibers, the aqueous effluent is concentrated and enzymatically oxidized in the presence of oxygen in order to polymerize the solubilized wood material and in order to increase its concentration. Lignin can be added to the water solubilized wood material or the solubilized wood material can be added to the fibers together with another binder. Eventually, the mixture is formed into a board and hardened.

Abstract: A new method for clarifying sugar cane juice is disclosed. Treating the phytic acid (or phytate) in sugar cane juice with phytase and acid phosphatase converts the phytic acid to inositol and inorganic phosphate, thereby improving the clarification of the sugar juice substantially. The valuable product inositol results as a consequence of the hydrolysis of phytic acid. The process is environmentally friendly, as it requires only the addition of small amounts of enzymes to existing sugar clarification processes. It does not cause inversion of sucrose to other sugars, and does not cause color formation. The extra phosphate released by this process is available to react with milk of lime, thus improving subsequent flocculation and consequent clarification.

Abstract: A biodegradable, non-toxic firefighting concentrate composition. The preferred compositions include 4 to 40 parts of a C.sub.16 -C.sub.18 tertiary amine having 2-10 ethoxy or other solubilizing groups per mol, 1 to 15 parts of a carboxylic acid having 6 to 16 carbon atoms; 1 to 6 parts of a C.sub.6 -C.sub.16 alcohol and 0 to 10 parts of C.sub.4 -and lower alcohols, and enough water to create a total of 100 parts by volume. The concentrate is usually diluted up to 100 times (v/v) with water, and is also effective when mixed with foam-forming materials. In addition, the composition is useful with soil bacteria for remediating soil contaminated with hydrocarbon fuel and for facilitating fuel dispersion and degradation within bacterial-action sewage systems.

Abstract: An aqueous composition containing an amine oxide surfactant, and preferably containing an amine oxide surfactant and enzymes, is added to crude oil, preferably by injecting the composition into the oil well, or adding the composition to pipelines or tankage containing the oil. An acid can also be used in conjunction with the composition. The composition acts to remove hydrocarbon contamination, from the well bore and mechanical components and further serves to break up the oil/water/sludge emulsion being delivered from the well head to facilitate separation of the oil. When used with an acid, the composition increases the effectiveness of the acid in removing inorganic contamination.

Abstract: Useful ingredients are extracted from a mycelium-containing culture medium by the steps of inoculating basidiomycetes in a solid culture medium containing bagasse as a substrate and proliferating mycelium, and then squeezing the solid culture medium containing proliferated mycelium to obtain a squeeze liquid (i), separately, adding water and beta 1,3, glucanase and at least one enzyme selected from the group consisting of chitinase, cellulase and protease to a mycelium-containing solid residue having been separated from the squeeze liquid (i), stirring the resulting mixture with maintaining the mixture at 30 to 60.degree.0 C. to lyse mycelial cell walls, and heating the resulting cell wall lysis product-containing liquid (ii) up to a temperature of 95.degree. C. to inactivate the enzyme and perform sterilization. The cell wall lysis product-containing liquid (ii) contains .beta.-glucan in a high concentration and shows excellent antitumor effects.

Abstract: The present invention relates to a method for industrial production of a peptide preparation having specific specifications by hydrolysis of a protein material, preferably based on whey. The method comprises several steps, which makes it easy to control the method so as to obtain a product which, e.g. because of low mineral content, is well suited for peritoneal dialysis and parenteral feeding. The method gives a high yield.

Abstract: An installation for the simultaneous separation and removal of fats and oils in waste water, which includes a treatment container chamber for receiving a liquid medium. Out of the treatment container chamber, through a bottom outlet, is an overflow pipe running upwards and which opens outwardly outside of the treatment container chamber as a discharge pipe, so that its inside bottom edge, at the point where it opens outwardly, defines a level of a medium in the treatment container chamber. A support material in the form of a plurality of plastic tubes is wound with lengths of polyester cord colonized with a fat and oil-degrading yeast microorganism Yarrowia lipolytica W1 in the treatment container chamber. A nutrient solution container, with a metering pump for feeding NH.sub.4+ into the treatment container chamber, as required, is included as a source of nitrogen for the fat and oil-degrading microorganism, along with a device for supplying the microorganisms with air.

Abstract: In a vertical down-flow fluid bed reactor, suspended particles in liquid proximal to an inlet in an uppermost part of the reactor are agitated to form a downward extending turbulent zone having vigorously moving particles and a non-turbulent zone distal to the inlet having essentially stationary particles in liquid below and adjoining the turbulent zone. In a vertical up-flow fluid bed reactor, an upward extending turbulent zone is formed proximal to an inlet in a lowermost part of the reactor and the non-turbulent zone is above the turbulent zone. The downward or upward extend of the turbulent zone is determined by the degree of agitation. The particles may contain an active substance and be in the form of a conglomerate of base particles having a desired density to control floatation or sedimentation. Particles in the turbulent and non-turbulent zones may be different such as having different specific gravities.

Abstract: This invention relates to an enzymatic detergent drain cleaner composition containing:0.015-20 wt % of an acid cellulase enzyme having hydrolytic activity specific to .beta.-glucosidic bonds;1-70 wt % of a water soluble carbonate salt;1-70 wt % of a water soluble acid that reacts in an aqueous medium with the carbonate salt to form carbon dioxide that dissolves in the aqueous medium;0.1-10 wt % of a surfactant; and0.05-5 wt % of a thickening agent. This detergent composition may be used as an enzymatic detergent drain cleaner or in a method for removing or preventing bacterial cellulose deposits in an aqueous system at a solution temperature of up to about 60.degree. C. and a pH of about 2 to about 7.