Abstract: Assemblies, systems, and methods for isolation of target material are provided. In various embodiments, an assembly for target material isolation includes a housing having an upper portion and a lower potion together defining an inner chamber. The inner chamber includes a semi-toroidal shape and the semi-toroidal shape defines a longitudinal axis. The assembly further includes one or more fluidic connection from the exterior of the housing to the inner chamber. An isolation material (e.g., polymer wool and/or magnetic beads) may be disposed within the inner chamber. A system includes a configured to fit at least a portion of the housing and releasably couple the assembly. Upon activation of the motor, the assembly may rotate about the longitudinal axis. An angle of the platform may be adjustable to thereby change the angle of the longitudinal axis about which the assembly rotates.

Abstract: A device for visual quantification of an amount of target species in a sample solution. The device includes an inlet, a separator, and a trap. The inlet is arranged to receive the sample solution. The separator in fluid communication with the inlet and arranged to separate one or more species from the sample solution. The trap is arranged downstream of the separator, in fluid communication with the separator, and arranged to trap one or more species of the sample solution. The trap is arranged such that the trapped species is visible for determination of the amount of target species in the sample solution.

Abstract: The present subject matter describes system (100) for magnetic enrichment of magnetically marked analytes. The system has capture chip (102) comprising a sample chamber (104) for holding sample having magnetically marked analytes, and a recovery chamber (106) connected to the sample chamber by a channel (202). The volume of recovery chamber is smaller than volume of sample chamber (104). The system has magnetic arrangement (108) comprising a set of magnets (110) in which each two adjacent magnets have opposite polarities facing sample chamber. Set of magnets has dimensions that at least conform to coverage area of sample chamber. The magnetic arrangement also has at least one recovery magnet (112) having dimensions conforming to coverage area of recovery chamber and on alignment, the at least one recovery magnet (112) is at a distance farthest away from the recovery chamber. The system has a linear positioner (114) for moving the magnetic arrangement.

Abstract: Acid mine drainage is treated with borohydride under conditions to yield highly pure iron nanoparticles. The resulting, unique, iron nanoparticles are also described.

Abstract: An apparatus for detecting a disease in a biological subject comprises a delivery system and at least two sub-equipment units which are combined or integrated in the apparatus, wherein the delivery system is capable of delivering the biological subject to at least one of the sub-equipment units and each sub-equipment unit is capable of detecting at least one property of the biological subject. Methods for detecting a disease with the apparatus are also provided.

Abstract: The present disclosure provides an electron generation apparatus includes: a discharge pin module having a support plate and a plurality of discharge pins coupled to the support plate; a discharge plate module disposed to be spaced apart from the plurality of discharge pins; a discharge plate slidably coupled inside the discharge plate module; a support structure having a coupling plate to which the discharge pin module and the discharge plate module are detachably coupled; and a circuit module having a main board located at a side opposite to the discharge pin module with the coupling plate being interposed therebetween and a plurality of distributed processing boards connected to the main board to apply a high-voltage, high-frequency pulse power to the plurality of discharge pins individually.

Abstract: The present invention is directed towards an apparatus and methods for a precise, fast and easy to use manipulation of beads. This method is particularly useful to carry out separation between the beads and the remaining supernants present in the fluid, maximizing the collection and purification efficiencies in tips for liquid handling.

Abstract: Provided are techniques that may include a disposable magnetic cell isolation holder having one or more passages that accommodate a magnetic retention material to facilitate magnetic cell isolation without adjustment of magnetic field parameters between isolation procedures using different magnetic particle sizes. When the magnetic cell isolation holder is coupled to a magnetic field generator, a first passage corresponding to a smaller particle size is positioned in a magnetic field where the magnetic field characteristics are different relative to a second passage, of a second holder or in the same holder, for use with another bead size.

Abstract: The invention relates to a processing device (110) and a method for manufacturing such a device. In a preferred embodiment, a mixture of magnetic particles (MP), a matrix material, and a volatile carrier is deposited onto binding sites (112) of a reaction surface (113). The deposited mixture is then dried while the magnetic particles (MP) are pulled away from the reaction surface (113) by a magnetic field (B). Thus unspecific binding of magnetic particles to the binding sites can be prevented.

Abstract: Microfluidic probe head for processing a sequence of separate liquid volumes separated by spacers. The microfluidic probe head includes: an inlet, an outlet, a first fluid channel and a second fluid channel and a fluid bypass connecting the first fluid channel and the second fluid channel. The first fluid channel delivers the sequence of separate liquid volumes from the inlet toward a deposition area, the fluid bypass allows the spacers to be removed from the first fluid channel obtaining a free sequence of separate liquid volumes without spacers, the first fluid channel delivers the free sequence of separate liquid volumes to the deposition area, and the second fluid channel delivers the removed spacers from the fluid bypass to the outlet. The present invention also provides a microfluidic probe and method for processing a sequence of separate liquid volumes.

Abstract: A connection assembly is disclosed for connecting a magnetic separator into a central heating system circuit, the magnetic separator including a housing, a separation chamber within the housing, and externally threaded inlet and outlet ports extending from the housing. The connection assembly comprises: a fitment adapted to be received within at least one of the ports of the separator, the fitment including a bore for carrying fluid from/to the central heating circuit to/from the separator; a threaded connector disposed around the bore for securing the fitment to the or each port, the threaded connector having a hand grip area for facilitating tightening of the connector by hand; and a removable force transfer element receivable between the fitment and the threaded connector for transferring force between the threaded connector and the fitment.

Abstract: A subsea oil filtration system is described that forms a closed circuit with and is mounted on or connected to a subsea rotating machine. According to some embodiments, the oil filtration system enables the removal of dirt and/or water that has entered the barrier fluid/oil system whether it comes from the Hydraulic Pressure Unit (HPU), from the umbilical or from other parts of the oil contained lubrication and/or barrier fluid systems.

Abstract: An energy storage assembly includes the following: at least one electrochemical cell, wherein the at least one electrochemical cell comprises an anode that is liquid during operation, an electrolyte that is liquid during operation, and a cathode that is liquid during operation; and a magnetic field-generating structure disposed outside the at least one electrochemical cell for generating a magnetic field, wherein the magnetic field-generating structure is configured such that the generated magnetic field penetrates the at least one electrochemical cell.

Abstract: An auto cleaning system for cleaning a drilling fluid in a drilling operation, includes a controller configured to determine the difference between a pressure reading by a first pressure sensor and a second pressure sensor is above a predetermined threshold value, and at least partially open an entrance valve on a second line to divert drilling fluid intake from a first line to the second line, and close the entrance valve on the first line to stop the drilling fluid from entering the first line.

Abstract: An automatic analysis device and method having a BF separation process, wherein the width in a container conveyance direction of a surface facing a reaction container of a magnet for preliminary magnetic collection of a first magnetic generation part (32p) is set to have a length including a region for housing a liquid sample of the reaction container conveyed to a magnetic collection position of the first magnetic generation part. An end in the container conveyance direction of a surface facing the reaction container of a magnet for regular magnetic collection of a second magnetic generation part (32m) is designed to be close to the center of the region for housing the liquid sample of the reaction container conveyed to a magnetic collection position of the second magnetic generation part.

Abstract: A method for treating ammonia-containing water containing ammonia, metal ions that form a complex with ammonia, and a reducing substance includes a first step of oxidizing the reducing substance by adding hydrogen peroxide to the ammonia-containing water; a second step of aerating the resultant water treated in the first step to remove the ammonia; a third step of forming an insoluble solid at a pH of 7 to 12 by adding at least one compound selected from the group consisting of iron salts, aluminum salts, calcium salts, and calcium hydroxide to the water treated in the second step; and a fourth step of separating the insoluble solid formed in the third step by solid-liquid separation.

Abstract: The present invention relates to a process for the separation of at least one first material from a mixture comprising this at least one first material and at least one second material.

Abstract: A technique relates to a machine for sorting. A removable cartridge includes a nanofluidic module. The removable cartridge includes an input port and at least two output ports. The nanofluidic module is configured to sort a sample fluid. A holder is configured to receive the removable cartridge. A pressurization system is configured to couple to the input port of the removable cartridge. The pressurization system is configured to drive the sample fluid into the nanofluidic module for separation to the at least two output ports.

Abstract: A system for monitoring machine fluids is provided. The system includes a fluid handling unit configured to pump the fluid and the fluid handling unit includes at least one fluid handling element. A magnetic material on the at least one fluid handling element is configured to generate a magnetic field. A sensing element located within the magnetic field is configured to measure fluctuations in the magnetic field.

Abstract: An intermediate medium heat exchanging device for a supercritical water oxidation system includes a material main loop, an intermediate medium loop and a replenishment branch. A material pump, a preheater, a spray desuperheater, a reactor and a regenerator are connected in sequence to form the material main loop; a buffer tank, a circulating pump, the regenerator, the preheater and a water cooler form the intermediate medium loop. A back pressure valve is located above the buffer tank. The replenishment branch includes a cooling water pump, an outlet of the cooling water pump is divided into two sub-branches, one sub-branch is connected with the spray desuperheater, and another sub-branch is connected with the buffer tank. The intermediate medium heat exchanging device is reasonable in structural design, and is able to maximumlly reduce the investment cost and ensure the stable operation of the system.

Abstract: There are provided a radiofrequency device for increasing amount of a bioactive substance in a plant cell and a plant cell culture method for increasing amount of useful intracellular secondary metabolites by using the radiofrequency device. The cell culture method of the present invention makes it possible to increase specific secondary metabolites such as daidzein, equol, and the like in a cell and thus can be used for development into various medicines, agricultural pesticides, spices, pigments, food additives, and cosmetics containing bioactive substances. Further, the cell culture method of the present invention improves conventional cell culture methods limitedly used for specific cells or specific metabolites for increasing amount of intracellular bioactive substances and thus can be widely applied to production of cells and secondary metabolites.

Abstract: Systems and methods for treating a wastewater are provided. The wastewater treatment system and methods allows for effective treatment of wastewater during conditions that may cause increased wastewater flow to the system.

Abstract: A method to continuously clean a fouled process stream using a magnetic field comprising feeding the fouled process stream, comprising a fouling constituent, to a magnetic separation unit. The magnetic separation unit comprising a separation vessel configured to receive the fouled process stream, a mounted magnet configured to generate the magnetic field operable to reduce a concentration of the fouling constituent. The mounted magnet comprising a magnet motor configured to rotate a shaft, the shaft configured to rotate a magnet, and the magnet configured to generate the magnetic field. A circulation pump fluidly connected to the separation vessel and a sampling point configured to allow removal of a sample. The method further includes measuring the concentration of the fouling constituent in the sample and supplying an effluent stream from the separation vessel to a clean collection vessel.

Abstract: A water treatment apparatus including: discharge treatment units each including a ground electrode and a discharge electrode opposing the ground electrode, and water to be treated is treated by forming a discharge between the ground electrode and the discharge electrode, and generating ozone by the discharge, and moreover causing the water to be treated to contact the discharge; a water reservoir portion that collects, in the interior of the treatment tank, the water to be treated having been subject to water treatment by one of the discharge treatment units; and an ozone supply section that supplies the ozone in the treatment tank to the water to be treated in the water reservoir portion are provided, and wherein the water to be treated passes through the plurality of discharge treatment units as a continuous flow.

Abstract: A process for separating clean water which has an impurities content of less than 100 ppm from waste materials such as farm manure is disclosed. In a first step, a plate separator splits the waste material stream into a first effluent fraction and a first solids fraction. The first solids fraction then undergoes two stages of pressing to increase the solids contents to about 30 to 40 percent which makes it suitable for granulation or pelletizing. The first effluent fraction is treated in successive stages with various chemical agents resulting in the removal of most of the solids and inorganic impurities from the effluent to achieve drinkable water purity.

Abstract: Mixed chloride-bromide bismuth oxyhalide compounds, with the molar ratio chloride:bromide being equal to or greater than 1:1, in the form of microspheres exhibiting flower-like surface morphology, are disclosed. Processes for preparing the compounds, formulations of the compounds and a method for purifying water using said compounds are also disclosed.

Abstract: A reconfigurable microfluidics multiplexing device for biological and chemical sample analysis which comprises: a cartridge comprising one or more sample fluid tracks (both horizontal and vertical) in a single plane, an array comprising one or more electronically programmable valves capable of being configured to alter fluid steering in the fluid tracks; a means for the array to interface with the fluid tracks; a means to control fluid flow in the fluid tracks; and a user interface to direct control of valves via control logic within the device; wherein, by the interface, valve positions are controllable and alterable.

Abstract: A method of cutting metallic foams that eliminates the problem of smeared surfaces is provided. The method involves infiltration of the foam with another material to serve as a support structure to the foam when being cut. The method can be executed using softer polymeric materials such as waxes, which are then frozen for machining. These materials are subsequently heated and removed from the foam. In a similar manner, epoxy material can be used, which requires no freezing. In this method, the epoxy material is burnt from the foam upon completion of machining. The method allows for machining foams using conventional machining processes, rather than non traditional methods such as electrical discharge machining.

Abstract: A sample analyzing method for suppressing an adverse effect on detection accuracy of a detection target substance is disclosed. In the sample analyzing method, a magnetic particle as a support of a detection target substance is transported by magnetic force to a second liquid container through a passage between a first liquid container storing a first liquid containing the magnetic particle and the second liquid container storing a second liquid containing a labeled substance to forma complex with the detection target substance and the magnetic particle. The complex formed in the second liquid container and containing the detection target substance, magnetic particle, and labeled substance is transported to a third liquid in a flow path, and the magnetic particle is transported to a detection tank for detecting the detection target substance, while being agitated in a mixed liquid of the complex and third liquid within the flow path.

Abstract: The present invention provides centrifuge/magnet-based analyzers and methods of operating thereof. The analyzer comprises three discs sandwiched together, in which each disc has difference functions. The top disc comprises magnetic units configured in patterns, whereas the bottom disc comprises tracks and magnetic units free to move in the tracks. The magnetic field co-generated by the top disc and the bottom disc attracts the magnetic beads in the intermediate disc to move and thus facilitates the reactions in the intermediate disc.

Abstract: A device, method and system is provided for binding particles for the separation and/or isolation of biological materials. In particular, a container is provided including a suspension of binding particles for the isolation of biological material, inner walls forming an elongate groove at the bottom of said container, and a cover having openings and/or being penetrable for a linear arrangement of multiple pipets or pipet tips, said openings being located above and parallel to said elongate groove.

Abstract: Methods for using vibration to harvest cells grown in 3D culture are provided. The methods entail the application of force to cells attached to a 3D matrix of sufficient amplitude, frequency, and duration to detach cells from the matrix and to flush the detached cells out of the matrix material. An apparatus for performing the methods of the invention as provided.

Abstract: The invention relates to a device and a method for fluidic cell guidance for flow cytometry or analyte enrichment. This allows magnetically marked analytes, in particular cells (1), to be dynamically enriched and individually detected in the flow from a sample, in particular magnetoresistively. For cell guidance, guiding ridges (12) are arranged in a flow channel (100), and so, in addition to a magnetic enrichment force (10z) and the shearing force of the flow (10y), a deflecting force (10x) caused by the fluidic obstacles (12) also acts on the cells (1) to be detected.

Abstract: A method for diagnosing a biomarker using magnetic particles and quantum dots for quantitative analysis and a biomarker diagnosis kit are provided. The method for diagnosing a biomarker includes: ii) providing magnetic particles having surfaces to which a primary antibody capable of collecting a biomarker using a linker is fixed; ii) providing quantum dots having surfaces to which a secondary antibody capable of detecting the biomarker is fixed; iii) sandwich-targeting the biomarker by the magnetic particles and the quantum dots; iv) selectively separating quantum dots sandwich-targeting the biomarker among the quantum dots; and v) quantifying the concentration of the biomarker by measuring absorbance or intensity of fluorescence of separated quantum dots.

Abstract: The invention relates to a system and a method for the detection of target components (102) in a sample with the help of indicator particles (101) distributed in said sample. The distance (d) between indicator particles (101) and a contact surface (112) is determined after the target components could bind to the contact surface and/or the indicator particles. Thus it is possible to detect how many target components (102) are bound without a need for a binding between indicator particles (101) and contact surface (112). Optionally the indicator particles (101) can be affected by a modulated force, e.g. via an electromagnet (141). The determination of the distance (d) between indicator particles (101) and contact surface (112) may for example be achieved by frustrated total internal reflection, measurement of magnetic fields, or FRET.

Abstract: The ability to levitate, to separate, and to detect changes in density using diamagnetic particles suspended in solutions containing paramagnetic cations using an inhomogeneous magnetic field is described. The major advantages of this separation device are that: i) it is a simple apparatus that does not require electric power (a set of permanent magnets and gravity are sufficient for the diamagnetic separation and collection system to work); ii) it is compatible with simple optical detection (provided that transparent materials are used to fabricate the containers/channels where separation occurs; iii) it is simple to collect the separated particles for further processing; iv) it does not require magnetic labeling of the particles/materials; and v) it is small, portable.

Abstract: Provided are a multiple discrimination device and a method of manufacturing the same. According to the multiple discrimination device, a three-dimensional micro ferromagnetic pattern is optimally designed and arranged to allow a magnetic force applied to a discrimination-target particle to be discriminated to be well controlled to perform discrimination well. The method employs a semiconductor processing technology, thereby precisely manufacturing and allowing mass production.

Abstract: A magnetic bead trap-and-mixer includes a channel having openings at opposing ends, and a rotor adjacent to the channel and comprising a permanent magnet, wherein the rotor is adapted to apply a magnetic field to the channel of sufficient strength to direct the movement of magnetic beads therein. In aspects, the channel is straight and/or has narrowed end. In further aspects, the rotor generates in the channel areas of areas of strong magnetic fields alternating with areas of very weak magnetic fields and the strong magnetic fields extend entirely across the channel.

Abstract: Disclosed herein are systems and methods for using the controlled movement of magnetic particles using controlled magnetic fields in a fluidic device containing separated fluidic regions to detect analytes in solution by immunoassay, such as an enzyme-linked immunosorbant assay (ELISA) for various medical and scientific applications. In order to achieve sequential exposure to the different chemical environments required in an immunoassay, magnetic particles are driven through fluid-containing chambers separated by air-gaps that may take the form of air bubbles or small open-air separations, for example. Externally controlled magnets coupled to actuators draw the flow of magnetic particles through air-liquid interfaces produced by microfluidic surface tension at the air-gap, washing the particles.

Abstract: An adapter for a sliding head of a sample processing system is provided that includes a plate and a magnet mounting recess wall. The plate includes a top surface, a bottom surface, a front wall extending between the top surface and the bottom surface, and a back wall extending between the top surface and the bottom surface. The magnet mounting recess wall is mounted to the top surface. The magnet mounting recess wall is configured to accommodate a magnet of a sliding head of a sample processing system. At least a portion of the bottom surface is curved in a first direction from the front wall to the back wall and is concave relative to the top surface.

Abstract: The present invention relates to an improved treatment system for inactivating microorganisms. An ion control means generates ions and compensates for conductivity variations in a liquid medium surrounding a microorganism. This increases the microorganism susceptibility to inactivation, by way of an inactivation pulse. A high intensity pulse means used in combination with the ion control means, delivers the inactivation pulse. Exemplary embodiments include a field generator, which generates a field that encompasses the microorganism diminishing ability of the microorganism to regulate proper cellular function, and a pulse pattern switching means. The pulse pattern switching means repeatedly alternates between generating ions by way of the ion control means and delivering the inactivation pulse by way of the high intensity pulse means.

Abstract: A tape adaptor (8) includes a bottom plate (20) and a top plate (60) mounted to the bottom plate (20) with a space (40) defined between an upper side wall (26) of the bottom plate (20) and a lower face (68) of the top plate (60) for receiving a tape segment (100) remaining on or cut from a continuous array tape (10). The tape segment (100) includes an array of wells (54) between two edges (52). A slit (46) is formed between an end (62) of the top plate (60) and an end (22) of the bottom plate (20). The tape segment (100) is slideably extended through the slit (46) into the space (40) with the edges (52) sliding on two ridges (38) on two longitudinal edges (36) of the bottom plate (20). The bottom plate (20) can include at least one groove (30) to receive the array of wells (54) of the tape segment (100). The tape segment (100) is positioned relative to the tape adaptor (8) by the ridges (38) or the groove (30).

Abstract: An apparatus for mixing and separating magnetic particles in a liquid comprises a holder having a plurality of apertures configured as an array of rows and columns and a plurality of containers capable of receiving liquid containing magnetic particles, each container being sized to be placed in one of the apertures; plural magnets capable of being moved relative to the containers between a first position and a second position to change the position of the magnets and magnetic particles in the container; and a drive mechanism for moving the magnets between positions at a sufficiently high speed that the particles do not settle down due to gravitational forces during motion between the first and second positions.

Abstract: The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.

Abstract: A method providing fluid treatment along a flow path extending through a conduit having a serial coupling of conduit segments including a non-magnetically conductive segment positioned between two non-magnetically conductive segments utilizing magnetic energy concentrated adjacent to the non-magnetically conductive conduit segment and at least one region of pulsed fluid treatment within a treatment vessel.

Abstract: A method and an apparatus for hydrolyzing an organic solid are described. The method includes mixing an organic solid and a nanobubble water having a plurality of nanobubbles, to form an organic liquid, in which the nanobubbles contain a combustible gas; and applying an ultrasonic wave on the organic liquid, such that the nanobubbles generate an additional cavitation effect. A preprocessor is applicable to an organic solid processing system having an anaerobic digestion tank, in which the anaerobic digestion tank has anaerobic microbes for generating a combustible gas. The preprocessor includes a nanobubble water generator, a digestion tank, and an ultrasonic wave generator. With the method and the structure, the nanobubbles are used to increase the probability of generation of the additional cavitation effect, and the combustible gas is used to improve an impact force of bursts produced by the cavitation effect.

Abstract: Described herein are methods and systems for harvesting, collecting, separating and/or dewatering algae using iron based salts combined with a magnetic field gradient to separate algae from an aqueous solution.

Abstract: The disclosure relates to flow cytometry. A method for precise individual cell detection and cell measurement of cells in the flow is disclosed. A pair of magnetoresistive components are used to produce a characteristic measuring signal profile from which the following information can be obtained: number of measurement deviations, measurement deviation distances, measurement deviation amplitudes, measurement deviation direction and measurement deviation direction sequence. The flow speed and the cell diameter can also be determined. Also, the signal noise ratio can be determined using the measurement deviation amplitude.

Abstract: Apparatus, systems and methods for subjecting biological contaminated liquid fuels and/or liquids to an oscillating electric field for an extended duration within a treatment vessel that has a defined cavity having an insulating fill material encasing a number of insulating flow tubes each having at least one conductive body therein. The defined cavity also has one or more reservoirs in communication with the insulating flow tubes. An oscillating electric field is generated within the insulating flow tubes and reservoirs, whereby a biologically contaminated liquid fuel and/or lubricant is subjected to this oscillating electric field for an extended time to inhibit proliferation of these biological contaminants within the liquid fuels and/or lubricants.

Abstract: A method can include applying a magnetic field to a well screen, thereby varying sizes of pores via which fluid flows through the well screen. A well screen can include a magnetic shape memory material having a dimension which changes in response to application of a magnetic field. Restriction to flow through the well screen can vary in response to the change in dimension of the magnetic shape memory material.