With Cell Rupturing Or Liberation Of Contained Liquids Patents (Class 241/2)
  • Patent number: 9707528
    Abstract: The present invention relates to containers for holding liquid samples. The containers may be useful for mixing a liquid sample or lysing cells in a liquid sample. The invention also relates to methods of using the containers of the invention.
    Type: Grant
    Filed: October 12, 2012
    Date of Patent: July 18, 2017
    Assignee: T2 Biosystems, Inc.
    Inventors: Adam Suchocki, Mark John Audeh, Matthew Blanco, James Franklin Chepin, Vasiliki Demas, Marilyn Lee Fritzemeier, Thomas Jay Lowery, Jr., Michael Min, Lori Anne Neely, Charles William Rittershaus, Hwa-Tang Wang, Parris Wellman
  • Patent number: 9243984
    Abstract: The invention relates to a method for decomposing a biological sample (9), the method having the following steps: the sample (9) is put into a container (6) which is composed of plastic, in particular, the container (6) is inserted into an adapter (2, 2a), the adapter with the closed container therein is connected to an apparatus which moves the adapter back and forth, in particular upwards, in automated fashion. This method makes it possible to decompose biological samples in automated fashion, to be precise both samples at room temperature and frozen samples. The invention also relates to an apparatus for carrying out the method, the apparatus having an adapter (2, 2a) which is predominantly composed of plastic and has sleeves (4) which are composed of metal and are intended to accommodate containers (6) which are composed of plastic, in particular.
    Type: Grant
    Filed: August 6, 2009
    Date of Patent: January 26, 2016
    Assignee: QIAGEN GMBH
    Inventors: Gerhard Baer, Dennis Mertens, Andreas Schaefer, Friederike Wilmer
  • Patent number: 9192939
    Abstract: A device and a method for preparing adipose tissue for transplantation from lobular fat material extracted by liposuction, the fat material consisting of a mixture of fluid materials and cell fragments, cells and one or more cell magroagglomerates of heterogeneous sizes, include at least one sterile container having a size reducing device for reducing the fat material into cell agglomerates of smaller and identical or similar sizes, the device dividing the inner chamber of the container into at least two portions, particularly a first upper portion and a second lower portion.
    Type: Grant
    Filed: March 22, 2011
    Date of Patent: November 24, 2015
    Assignee: LIPOGEMS INTERNATIONAL S.P.A.
    Inventor: Carlo Tremolada
  • Patent number: 8960582
    Abstract: A micro-spike algae harvesting and biofuel extraction system including a micro-spike board supported at an inclined angle by a rigid framework and forming a planar substrate for support of an array of miniature, upwardly pointed, conically-shaped spikes, and a sprinkling system for dispersing an algal solution of algae cells from above the micro-spike board onto the array of spikes for puncturing the algae cells to release biofuel contained therein.
    Type: Grant
    Filed: April 27, 2012
    Date of Patent: February 24, 2015
    Inventors: John Kelson, Benjamin J. Pavlik
  • Publication number: 20150010951
    Abstract: The present invention relates to a method of enriching for membranous microvesicles relative to the cellular population in a biological sample. More particularly, there is provided a method for enriching for exosomes from plasma. In a related aspect, there is provided a method of reducing the concentration of cellular and cellular derived molecules in a biological sample. Still further, the present invention provides methods for selectively isolating mRNA subpopulations from exosomes. Yet further, there are provided methods of amplifying exosome derived RNA. The method of the present invention is useful in a range of applications including, but not limited to, diagnostic, prognostic, therapeutic, research and development applications, to the extent that the enrichment of exosomes is required.
    Type: Application
    Filed: October 26, 2011
    Publication date: January 8, 2015
    Applicant: Clinical Genomics Pty. Ltd.
    Inventors: Lawrence Charles LaPointe, Susanne Kartin Pedersen, Aidan McEvoy
  • Patent number: 8882012
    Abstract: A cell isolation apparatus includes: a chamber body which includes a chamber into which tissue is to be introduced; an isolation member which is moved in the chamber and which is to collide with the tissue to isolate a cell; and a controlling portion which controls an operation of the isolation member in the chamber body.
    Type: Grant
    Filed: August 25, 2011
    Date of Patent: November 11, 2014
    Assignees: Nihon Kohden Corporation, Tokyo Women's Medical University
    Inventors: Yuji Haraguchi, Tatsuya Shimizu, Teruo Okano, Takahiro Shioyama, Akane Suzuki, Sunao Takeda
  • Publication number: 20140154396
    Abstract: The present invention relates to plant cells and plants which synthesize hyaluronan and to methods for preparing such plants, and also to methods for preparing hyaluronan with the aid of these plant cells or plants. Furthermore, the present invention relates to the use of plants for preparing hyaluronan and to food or feed which comprises hyaluronan.
    Type: Application
    Filed: September 23, 2013
    Publication date: June 5, 2014
    Applicant: BAYER CROPSCIENCE AG
    Inventors: Claus FROHBERG, Rainhard KOCH
  • Publication number: 20140068808
    Abstract: The present invention provides an inbred corn line designated ID3517, methods for producing a corn plant by crossing plants of the inbred line ID3517 with plants of another corn plant. The invention further encompasses all parts of inbred corn line ID3517, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line ID3517, and plants produced according to these methods.
    Type: Application
    Filed: August 30, 2012
    Publication date: March 6, 2014
    Applicant: SYNGENTA PARTICIPATIONS AG
    Inventor: Scott Kelly
  • Publication number: 20140053829
    Abstract: A method of and system for producing oil and valuable byproducts from grains, such as corn, in dry mills are disclosed. The method and system include dewater milling process after fermenting. Further, the method and system are able to produce oil without evaporating. Moreover, the method and system include one or more of the germ processing units, emulsion processing units, fiber processing units, high value protein producing units, and glycerol and inorganic salt producing units, such that high value byproducts are able to be generated.
    Type: Application
    Filed: August 20, 2013
    Publication date: February 27, 2014
    Inventor: Chie Ying Lee
  • Publication number: 20140008470
    Abstract: A method for crushing and drying damp wood, wherein the wood is introduced into an impact reactor, which has a substantially cylindrical main body, in the closed interior space of which there rotates a rotor with at least one impact element, which contacts the wood and crushes it into part-constituents while generating a great transfer of momentum. The interior space of the cylindrical main body is assigned a suction removal device, which during the impact crushing removes the mist of moisture produced when the impact element makes contact with the material, and the interior space of the cylindrical main body has admitted to it hot gas, heated by waste heat from a combustion process, at a temperature of less than 95° C., in particular less than 80° C., or the exhaust gas from a combustion process, which after a collision of the impact element with a material constituent penetrates the material constituent in place of the moisture leaving. Also a device for carrying out the method.
    Type: Application
    Filed: February 9, 2012
    Publication date: January 9, 2014
    Inventor: Ralf Schaefer
  • Patent number: 8579852
    Abstract: Methods and devices to process harvested skin tissue and to immediately reintroduce the ground tissue into the patient to repair a tissue defect are disclosed. A hand-held portable tissue grinder comprising a housing and a grinding element are disclosed. The tissue grinder is used for grinding skin and subcutaneous tissue and includes a sterile polymer housing having a first opening adapted to receive the skin and subcutaneous tissue. The tissue grinder also includes a sterile grinding element disposed substantially within the housing. The grinding element includes a plurality of cutting surfaces adapted to operably engage with the housing to provide ground tissue and further includes a handle mechanism coupled to the grinding element to move the grinding element with respect to the housing to grind the skin and subcutaneous tissue, wherein the ground tissue can be removed from the housing.
    Type: Grant
    Filed: February 19, 2010
    Date of Patent: November 12, 2013
    Inventor: Omeed Memar
  • Patent number: 8573518
    Abstract: The invention relates to a method for breaking down biological material, especially for obtaining biomolecules by ultrasound, the biological material being arranged, in a container together with a liquid.
    Type: Grant
    Filed: April 24, 2009
    Date of Patent: November 5, 2013
    Assignee: QIAGEN, GmbH
    Inventors: Thomas Rothmann, Claudia Schmid, Andreas Schafer, Gabriele Christoffel
  • Publication number: 20130217010
    Abstract: The present invention relates to containers for holding liquid samples. The containers may be useful for mixing a liquid sample or lysing cells in a liquid sample. The invention also relates to methods of using the containers of the invention.
    Type: Application
    Filed: October 12, 2012
    Publication date: August 22, 2013
    Applicant: T2 Boisystems, Inc.
    Inventor: T2 Biosystems, Inc.
  • Publication number: 20130121891
    Abstract: A process is disclosed which includes separating selected solids from a process stream with a selected solids mechanical processor containing a filter to produce a filtrate substantially free of selected solids and a retentate containing recovered selected solids. In one embodiment, the process stream contains an increased amount of liquid is provided to a downstream mechanical processor as compared to a process stream having no selected solids mechanical processor upstream. The selected solids mechanical processor can be a dynamic filtration device or a static separation device. Bio-oil recovery efficiency, quantity, and quality can be improved as a result.
    Type: Application
    Filed: October 31, 2012
    Publication date: May 16, 2013
    Applicant: ICM, Inc.
    Inventor: ICM, Inc.
  • Patent number: 8356763
    Abstract: Methods and arrangements to lyse a biological sample are described. The arrangements comprise a lysis tube containing the sample, one or more electromagnets generating a magnetic field, and one or more permanent magnets inside the lysis tube. The permanent magnets move and lyse the sample when a magnetic field is generated by the electromagnets.
    Type: Grant
    Filed: March 22, 2012
    Date of Patent: January 22, 2013
    Assignee: California Institute of Technology
    Inventors: Aditya Rajagopal, Axel Scherer, Mladen Barbic
  • Patent number: 8348183
    Abstract: The invention relates, for example, to methods for preparing a plant or animal sample for processing, i.e. for example for the isolation of nucleic acids or proteins from the sample, as well as to a pulverizer.
    Type: Grant
    Filed: April 2, 2008
    Date of Patent: January 8, 2013
    Assignee: Qiagen GmbH
    Inventors: Dennis Mertens, Thomas Voit
  • Publication number: 20130001333
    Abstract: A method for processing rapeseed includes dehulling and separating the rapeseed into kernel fractions and husk fractions. The kernel fractions are subjected to one or more pressings so as to obtain oil and an oil cake, the oil cake having solid content and oil content. The oil cake is subjected to at least one further milling process so as to provide an output that is a base material, filler material or additive for human foods.
    Type: Application
    Filed: September 11, 2010
    Publication date: January 3, 2013
    Inventors: Michael Rass, Christian Schein
  • Publication number: 20130005955
    Abstract: A micro rotary machine may include a micro actuator and a micro shaft coupled to the micro actuator. The micro shaft comprises a horizontal shaft and is operable to be rotated by the micro actuator. A micro tool is coupled to the micro shaft and is operable to perform work in response to motion of the micro shaft.
    Type: Application
    Filed: April 16, 2012
    Publication date: January 3, 2013
    Applicant: MICROZEUS LLC
    Inventor: Harold Stalford
  • Patent number: 8343562
    Abstract: The present invention describes a method and apparatus for aqueous extraction of chemical compounds from solid material in which the solid material is entrained in an extraction liquid phase that flowed around immersed sonotrodes emitting radial or focused high energy ultrasonic waves. A significant increase in extracted material and decreases in extraction time is observed.
    Type: Grant
    Filed: December 18, 2007
    Date of Patent: January 1, 2013
    Assignee: Cavitus Pty Ltd
    Inventors: Darren M. Bates, Arthur R. McLoughlin, Andrew Sin Ju Yap
  • Patent number: 8287692
    Abstract: Cellulose nanofibers are produced using a 4-hydroxy TEMPO derivative by treating a cellulosic material with an oxidizing agent in water in the presence of a cellulose oxidation catalyst containing an N-oxyl compound to prepare oxidized cellulos, and microfibrillating the oxidized cellulose.
    Type: Grant
    Filed: December 25, 2008
    Date of Patent: October 16, 2012
    Assignee: Nippon Paper Industries Co., Ltd.
    Inventors: Shoichi Miyawaki, Shiho Katsukawa, Hiroshi Abe, Yuko Iijima, Akira Isogai
  • Patent number: 8286899
    Abstract: A tissue dicing and separation device comprises a housing having an interior that receives tissue for dicing and separation. The device includes a blade assembly connected to a motor and solenoid. The solenoid reciprocates the blade assembly while the motor clocks the blade assembly during each reciprocation cycle. This reciprocating and clocking action of the blade assembly dices and/or minces the tissue. The housing is filled with a fluid and then the blade assembly is continuously rotated to create a vortex within the housing. The vortex causes the diced tissue particles to separate by particle size. Outlets at different positions along the housing permit withdrawal of a slurry of fluid and tissue particles with the tissue particles having a desired size range. The obtained slurry may be used in conjunction with a fistula plug treatment device and method.
    Type: Grant
    Filed: May 11, 2010
    Date of Patent: October 16, 2012
    Assignee: Ethicon Endo-Surgery, Inc.
    Inventors: Joseph P. Schowalter, Prasanna Malaviya, David A. Witt, Frederick E. Shelton, IV
  • Patent number: 8268603
    Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.
    Type: Grant
    Filed: September 22, 2005
    Date of Patent: September 18, 2012
    Assignee: Cepheid
    Inventors: Michael T. Taylor, Phillip Belgrader, Farzad Pourahmadi, William A. McMillan, Ronald Chang, Jesus Ching
  • Patent number: 8234974
    Abstract: A manually actuated juicing tool used to insert directly into a pomegranate fruit cut horizontally in half. Once cut horizontally, the pomegranate reveals sections of arils separated by membranes. The tool has a rigid horizontal handle that the user grasps with one hand. A round mid-shaft projects down from the handle a distance sufficient to allow the tool to be projected down to the base of the husk of the fruit while leaving the handle above the husk. At the base of the mid-shaft are two or more tines projected outwardly and slightly downwardly. The tine at its outward end is convex to match the symmetry of the husk of the fruit. On the bottom of the tine are tiny cutting spikes used to pierce the arils. The tine also contains small vertical extrusion holes to allow juice to flow above the tine while leaving all solids beneath.
    Type: Grant
    Filed: November 20, 2008
    Date of Patent: August 7, 2012
    Inventor: Mark D. Hanlon
  • Patent number: 8231070
    Abstract: A device and method to provide a simplified wash process and controlled disintegration of a soft substance, such as a gel, are disclosed. In use, a block of, e.g., gel matrix is placed in the device and washed with a series of appropriate solutions to remove interfering contaminants. The washing liquid is removed through a deformable narrow opening in the bottom of the device, and, subsequently, the gel block is extruded through the deformable narrow opening, by a physical force, such as centrifugal force, a vacuum or positive pressure from a gas or liquid, etc., resulting in fragmentation of the gel block into a series of particles of similar size. The fragmentation of the gel results from shear forces exerted onto the gel block traveling through the deformable narrow opening in the device.
    Type: Grant
    Filed: May 29, 2007
    Date of Patent: July 31, 2012
    Assignee: Northeastern University
    Inventors: Alexander Lazarev, Thomas Rejtar, Barry L. Karger
  • Publication number: 20120175441
    Abstract: Methods and arrangements to lyse a biological sample are described. The arrangements comprise a lysis tube containing the sample, one or more electromagnets generating a magnetic field, and one or more permanent magnets inside the lysis tube. The permanent magnets move and lyse the sample when a magnetic field is generated by the electromagnets.
    Type: Application
    Filed: March 22, 2012
    Publication date: July 12, 2012
    Inventors: Aditya RAJAGOPAL, Axel SCHERER, Mladen BARBIC
  • Patent number: 8162247
    Abstract: A grinding system includes a mortar container having an open top portion and a bottom portion having a first interior grinding surface and a wall portion interconnecting the top and bottom portions for defining a hollow interior collection chamber, a modular pestle assembly including a universal pestle shaft having at its proximate end an actuator portion extending above the top portion and at its distal end a mounting portion for receiving one of a set of pairs of matching tips and pestle collars, the pestle tip having a second grinding surface for engaging the first grinding surface, and a closure device sealingly engageable with the pestle assembly and the open top portion for sealing the container and the collection chamber between the grinding surfaces and the top portion of the mortar container.
    Type: Grant
    Filed: August 17, 2010
    Date of Patent: April 24, 2012
    Assignee: Biomedical Polymers, Inc.
    Inventor: Michael Faulker
  • Publication number: 20120091235
    Abstract: Apparatus and methods for mechanical cell lysis with single cell resolution which requires very low applied pressure. The device can be handheld, simple to operate, requires no external power except for hand-applied pressure via a syringe, and is applicable to all cell types including yeast and bacterial cells. The device is also capable of mechanically lysing a single cell. A single cell is selected from a biological sample of interest. The single cell is lysed by application of mechanical stress in a single cell lysing apparatus having a trap structure for deterministically capturing the cell and a stress raiser that cooperates with a source of mechanical stress so as to apply sufficient force to rupture a cell. The stress raiser can be a properly designed edge of the trap or it can be a lithographically produced structure such as a nanoblade or a nanopillar.
    Type: Application
    Filed: September 13, 2011
    Publication date: April 19, 2012
    Applicant: California Institute of Technology
    Inventors: Zhenyu Li, Axel Scherer
  • Patent number: 8043496
    Abstract: A method and apparatus for extracting oil from algae comprises a disruptor (125) having a plurality of deflectors (220 and 225) against which a mixture of algae and water (100) is forcibly impacted upon the urging of a pump (115). The impacting of the algal cells against the deflectors ruptures their cell walls and liberates the lipids (oil) and other materials contained therein. A tank (130) collects the mixture and after a settling period, the mixture forms at least three layers comprising oil (155), water (160), and algal residue (165). The oil layer is removed through one or more conduits (177, 178) into a holding tank (185) for further refining and use. The water is discarded, and the biomass residue comprising algal cell walls and other non-oil components is removed to another holding container (199) from which it can be discarded or used as an agricultural fertilizer or the like. If desired, the residue can be further treated in order to scavenge any remaining oil.
    Type: Grant
    Filed: December 22, 2008
    Date of Patent: October 25, 2011
    Inventors: Peter Allen Schuh, Allen John Schuh
  • Patent number: 8020790
    Abstract: Improved ball mill disruption techniques. In different embodiments, disrupting particles that are not substantially spherical are used. In other embodiments, roughened disrupting particles are used. In other embodiments, larger disrupting particles are used. In each instance, improved disruption can be achieved.
    Type: Grant
    Filed: October 21, 2004
    Date of Patent: September 20, 2011
    Assignee: Applied Biosystems, LLC
    Inventors: Jon Kemppainen, Gary J. Latham
  • Patent number: 8016218
    Abstract: Disclosed is a linear shaker apparatus capable of effectively mixing a wide variety of large volume liquid samples that provides a near linear mixing motion so as to allow for the easy break up of solids suspended in the fluid sample. The longer shaking distances and slower operating speeds employed by the linear shaker described herein, which resembles the motion a scientist or lab technician might employ when shaking such a vial by hand, does not lend itself to the electromagnetic technique employed in various prior art shakers. The linear shaker described herein employs an electric motor and linkage that allows varying the speed and stroke of the mixing operation to accommodate different laboratory protocols.
    Type: Grant
    Filed: March 16, 2011
    Date of Patent: September 13, 2011
    Inventor: Mitchell Friedman
  • Publication number: 20110167715
    Abstract: The invention provides low-cost, non-thermal methods to transform and beneficiate bulk materials, including low rank coals such as peat, lignite, brown coal, subbituminous coal, other carbonaceous solids or derived feedstock. High pressure compaction and comminution processes are linked to transform the solid materials by eliminating interstitial, capillary, pores, or other voids that are present in the materials and that may contain liquid, air or gases that are detrimental to the quality and performance of the bulk materials, thereby beneficiating the bulk products to provide premium feedstock for industrial or commercial uses, such as electric power generation, gasification, liquefaction, and carbon activation. The handling characteristics, dust mitigation aspects and combustion emissions of the products may also be improved.
    Type: Application
    Filed: March 11, 2011
    Publication date: July 14, 2011
    Applicant: GTL ENERGY, LTD
    Inventors: Robert R. French, Robert A. Reeves
  • Patent number: 7954741
    Abstract: Apparatus for grinding biological samples contained in tubes (12) carried by a turntable (10) driven with oscillating motion, the zone in which the tubes (12) oscillate being surrounded by cooling means (30) enabling the samples in the tubes (12) to be maintained at a desired low temperature, e.g. 4° C., while they are being ground.
    Type: Grant
    Filed: June 28, 2007
    Date of Patent: June 7, 2011
    Assignee: Bertin Technologies
    Inventors: Thierry Kunc, Jean-Jacques Bougy, Jean Boquet, Julien Vallayer, Emmanuelle Sorel
  • Patent number: 7902129
    Abstract: Lubrication substrates are disclosed that have been treated with or that carry a lubricant for maintaining a paper shredder. The lubrication substrate is fed through the shredding mechanism of a paper shredder. Rather than requiring disassembly of the paper shredder, maintenance and lubrication can be performed by passing the lubrication substrate through the shredding mechanism. The lubrication substrates can take any of a variety of forms, and can be substantially rigid and/or stiff. The lubrication substrates can also include scented lubricants and decomposing agents. Paper shredders are also disclosed for use with the lubrication substrates. The paper shredders can have various means for identifying when lubrication is needed and/or when the cutting mechanism of the paper shredder is lubricated by a lubrication sheet. Paper shredders having a reservoir of lubricant for delivery to the cutting mechanism of the paper shredder are also disclosed.
    Type: Grant
    Filed: November 27, 2006
    Date of Patent: March 8, 2011
    Assignee: Buttercup Legacy, LLC
    Inventor: Mark S. Allen
  • Publication number: 20110027447
    Abstract: The present invention relates to plant cells and plants, which are genetically modified, whereby the genetic modification leads to an increase in the activity of a starch-phosphorylating OK1 protein in comparison to the corresponding wild type plant cells or wild type plants that have not been genetically modified. In addition, the present invention concerns means and methods for the manufacture of such plant cells and plants. These types of plant cells and plants synthesise a modified starch. Therefore, the present invention also concerns the starches synthesised from the plant cells and plants according to the invention, methods for manufacturing these starches, and the manufacture of starch derivatives of these modified starches, as well as flours containing starches according to the invention. Furthermore, the present invention also relates to nucleic acids, coding starch-phosphorylating OK1 proteins, vectors, host cells, plant cells, and plants containing such nucleic acid molecules.
    Type: Application
    Filed: May 5, 2010
    Publication date: February 3, 2011
    Applicant: BAYER CROPSCIENCE AG
    Inventors: CLAUS FROHBERG, OLIVER KOETTING, GERHARD RITTE, MATIN STEUP
  • Patent number: 7785868
    Abstract: A standalone bench top laboratory instrument designed to disrupt, or lyse, cells, spores and tissue samples using ultrasonic energy. The lysing device is programmable, allowing the user control over the sample volume, sonication power level, and lysing duration in order to optimize lysing protocols for specific targets. Once a lysing protocol is entered, the device automatically lyses the sample according to the entered lysing protocol. The lysing device also provides a cooling feature, enabled by a heat exchanging sub-assembly, to prevent the sample from exceeding a maximum set temperature.
    Type: Grant
    Filed: November 29, 2005
    Date of Patent: August 31, 2010
    Assignee: Microfluidic Systems, Inc.
    Inventors: Bob Yuan, Allen Northrup, Farzad Pourahmadi
  • Patent number: 7785869
    Abstract: A sonication apparatus is directed to a microfluidic-based system to automate differential extraction of specific cell types within a mixed sample. The microfluidic-based system includes a sonication module for selective cell lysis, separating means to eliminate centrifugation, high surface area pillar chip modules to purify DNA from a cell lysate, and microfluidic circuitry to integrate the steps in an automated platform.
    Type: Grant
    Filed: February 9, 2006
    Date of Patent: August 31, 2010
    Assignee: Microfluidic Systems, Inc.
    Inventors: Phillip I. Belgrader, Bob Yuan
  • Publication number: 20100127104
    Abstract: Medication is disposed of by rending the medication, including medicaments stored in blister packs and transdermal patches, and aggregating the riven medication with a disposal medium that is suitable for burial or incineration. Burial or incineration of the aggregated disposal medium and the riven medication reduces the likelihood of water contamination.
    Type: Application
    Filed: January 21, 2010
    Publication date: May 27, 2010
    Inventor: Ann Wakeman
  • Patent number: 7611840
    Abstract: A device for sample tissue disruption and/or cell lysis comprising: a piezoelectric material; and at least a second material in contact with the piezoelectric material; and wherein the second material has an uneven surface on an opposite side to that in contact with the piezoelectric material. The device may be made by assembling at least three layers and membranes for the valves and pumps. The piezoelectric material is actuated by an external voltage source to generate cavitation, which disrupts tissue and/or lyses cells, in particular by a modulated alternative external voltage. The invention further provides a method of disrupting tissue and/or lysing cells in a device. Also provided is a piezoelectric device comprising a piezoelectric material in contact with a second material, and wherein the second material has an uneven surface on an opposite side to that in contact with the piezoelectric material.
    Type: Grant
    Filed: August 3, 2004
    Date of Patent: November 3, 2009
    Assignee: Agency for Science, Technology and Research
    Inventors: Guolin Xu, Pei-Lin Mao, Yuan Hong Yu, Eng Hock Francis Tay
  • Patent number: 7584909
    Abstract: A method for processing unseparated egg shells is provided. The method includes placing the unseparated egg shells in a fluid tank containing a fluid mixture, applying cavitation to the fluid mixture to thereby assist in separating the egg shell membranes from the egg shells, and recovering the egg shell membranes. Preferably, the fluid mixture is recirculated to thereby provide for continuous processing of unseparated egg shells. The method may further include drying the egg shell membranes to produce dried egg shell membranes which may then be vacuum packaged for storage and/or transport. The dried egg shell membranes may then be subjected to an extraction process for extracting at least one type of polypeptide from the egg shell membranes. Collagen, hyaluronic acid, or amino acids of interest may be extracted from the egg shell membrane and purified for numerous uses.
    Type: Grant
    Filed: January 17, 2006
    Date of Patent: September 8, 2009
    Assignee: Biova, L.L.C.
    Inventor: Vladimir Vlad
  • Patent number: 7491527
    Abstract: A differential extraction system is directed to a micro fluidic-based integrated cartridge to automate differential extraction of specific cell types within a mixed sample. The integrated cartridge includes a sonication module for selective cell lysis, separating means to eliminate centrifugation, high surface area pillar chip modules to purify DNA from a cell lysate, and micro fluidic circuitry to integrate the steps in an automated platform.
    Type: Grant
    Filed: September 17, 2004
    Date of Patent: February 17, 2009
    Assignee: MicroFluidic Systems, Inc.
    Inventors: Bob Yuan, Nina Aflatooni
  • Publication number: 20090039183
    Abstract: Medication is disposed of by rending the medication, including medicaments stored in blister packs and transdermal patches, and aggregating the riven medication with a disposal medium that is suitable for burial or incineration. Burial or incineration of the aggregated disposal medium and the riven medication reduces the likelihood of water contamination.
    Type: Application
    Filed: August 6, 2007
    Publication date: February 12, 2009
    Inventor: Ann Wakeman
  • Patent number: 7467754
    Abstract: An apparatus for comminuting biological specimens includes a receiving component provided with a drive source, a sample retainer configured for retaining at least one biological specimen and for engagement in the receiving component, and a single use comminution mechanism in operational relationship with the sample retainer. The drive source is configured for driving the comminution mechanism. A collector is associated with the receiving component for receiving comminuted product generated by the comminution mechanism. A method for comminuting biological specimens is provided so that, upon comminution, the specimen is readily subject to rapid extraction and detection of drugs and their metabolites, compounds, chemicals, pesticides, steroids, growth enhancers, contaminants or other pharmacologic agents which may reside in the specimen.
    Type: Grant
    Filed: May 20, 2004
    Date of Patent: December 23, 2008
    Assignees: Wahl Clipper Corporation, Drugrisk Solutions, LLC
    Inventors: James E. McCambridge, Scott A. Melton, Carl Salavka, Terri Stripling, Jeff Karker, Thomas M. Eden, III
  • Publication number: 20080302889
    Abstract: Sewage sludge that has been de-watered to 25% solids is subjected to a combination of shearing and heating that liquefies the sludge, and drops its viscosity to below 12,000 cP. The fact of liquefaction prepares the sludge such that subsequent drying can be done more cost-effectively than hitherto. After drying, the dried sludge can be incinerated.
    Type: Application
    Filed: March 3, 2008
    Publication date: December 11, 2008
    Inventors: Owen Patrick WARD, Ajay Singh
  • Publication number: 20080223962
    Abstract: Improved ball mill disruption techniques. In different embodiments, disrupting particles that are not substantially spherical are used. In other embodiments, roughened disrupting particles are used. In other embodiments, larger disrupting particles are used. In each instance, improved disruption can be achieved.
    Type: Application
    Filed: October 21, 2004
    Publication date: September 18, 2008
    Inventors: Jon Kemppainen, Gary J. Latham
  • Patent number: 7413135
    Abstract: The present invention relates to a high pressure homogenizing apparatus and a method thereof for finely dividing a fine solid material or fibrous cellulose of chemical, medical, and resin products in a suspension as a dispersion or emulsification, or finely dividing by crushing cell membranes of fungi with high efficiency. The apparatus of the present invention is free from damage of valves so that its maintenance and control become easy. The high pressure homogenizing apparatus to finely divide a raw material in the suspension includes a high pressure homogenizing device having an orifice, a raw material receiving passage connected to the high pressure homogenizing device, a processing piston, and a processing recess disposed in a receiver.
    Type: Grant
    Filed: January 10, 2007
    Date of Patent: August 19, 2008
    Inventors: Shigeo Ando, Masao Ando, Toyoroku Ando
  • Patent number: 7370819
    Abstract: In a biological sample grinding device and method, a bearing having a plurality of rolling elements disposed between a pair of rings is disposed in a container in which a fluid having at least one biological sample particle therein is introduced. With the bearing immersed in the fluid having the biological sample particle therein, one ring of the bearing is rotated relative to the other ring thereof, whereupon the rolling elements roll between the pair of rings and, in response thereto, the biological sample particle is drawn by the fluid between the pair of rings where it is subject to being crushed or ground between at least one of the rolling elements and at least one of the rings. A baffle can be disposed in the container for inhibiting the rotation of the fluid and, hence, the introduction of air into the fluid.
    Type: Grant
    Filed: September 26, 2006
    Date of Patent: May 13, 2008
    Assignee: Advanced Analytical Technologies, Inc.
    Inventor: Robert Czarnek
  • Patent number: 7337998
    Abstract: An apparatus for comminuting biological specimens includes a receiving component provided with a drive source, at least one sample retainer configured for accommodating at least one biological specimen and constructed and arranged for engagement in the receiving component. The sample retainer includes a first component configured for receiving a specimen prior to comminution and a second component for receiving the specimen after comminution. In addition, the sample retainer is insertable into the receiving component as a single piece.
    Type: Grant
    Filed: March 14, 2005
    Date of Patent: March 4, 2008
    Assignees: Wahl Clipper Corporation, DrugRisk Solutions, LLC
    Inventors: Scott A. Melton, James E. McCambridge, Steve Arndt, Dennis L. Miller, Terri Stripling, Jeff Karker
  • Publication number: 20070262181
    Abstract: In a biological sample grinding device and method, a bearing having a plurality of rolling elements disposed between a pair of rings is disposed in a container in which a fluid having at least one biological sample particle therein is introduced. With the bearing immersed in the fluid having the biological sample particle therein, one ring of the bearing is rotated relative to the other ring thereof, whereupon the rolling elements roll between the pair of rings and, in response thereto, the biological sample particle is drawn by the fluid between the pair of rings where it is subject to being crushed or ground between at least one of the rolling elements and at least one of the rings. A baffle can be disposed in the container for inhibiting the rotation of the fluid and, hence, the introduction of air into the fluid.
    Type: Application
    Filed: September 26, 2006
    Publication date: November 15, 2007
    Applicant: Advanced Analytical Technologies, Inc.
    Inventor: Robert Czarnek
  • Patent number: 7270284
    Abstract: A tissue homogenizer. The tissue homogenizer comprises a first chamber, a pair of blades, a first filter and a second filter. The first chamber has a first opening and a second opening. The blades are disposed in the first chamber. The first filter is disposed in the first chamber between the first opening and the blades. The second filter is disposed in the first chamber between the second opening and the blades. A tissue piece is placed between the first filter and the second filter cut by the blade, and moved by a fluid through the second filter to generate homogenized tissue pieces.
    Type: Grant
    Filed: June 29, 2004
    Date of Patent: September 18, 2007
    Assignee: Industrial Technology Research Institute
    Inventors: Chun-Jen Liao, Hua-Chun Hsu, Ko-Chun Hsu, Da-Pan Chen, Mei-Chiao Lin
  • Patent number: 7240862
    Abstract: Method for disrupting S. Cerevisiae yeast cells in an aqueous solution, by means of a continuous process under high pressure (up to 4000 bar), using a homogenizer. The suspension to be processed passes through a homogenizing valve with a “sharp edge” or “knife edge” passage head in order to achieve a 100% cell disruption rate with a single passage, at a dynamic pressure equal to or above 2000 bar. The “sharp edge” or “knife edge” profile of the passage head (4) is characterized by an inside diameter (9) of 10.9-14 mm and an outside diameter (10) of 11.9 mm-15 mm, and operates at a flow rate of 100-500 liters/hour and a dynamic pressure of more than 2000 bar.
    Type: Grant
    Filed: September 10, 2004
    Date of Patent: July 10, 2007
    Assignee: Niro-Soavi S.p.A.
    Inventors: Silvia Grasselli, Marco Gandini, Simone Grandi