By Electrophoresis Patents (Class 356/344)
  • Patent number: 10234378
    Abstract: A method and system for reducing the effect of distortions on the baseline signal in an absorption spectroscopy system used for the detection or measurement of chemical species in a medium, whereby one or more correlations or convolutions are performed on the signal using a kernel function. The shape of the kernel function is chosen to reduce the influence of the baseline distortions on the processed measurand determination. The kernel function may also be chosen to enhance the absorption signal.
    Type: Grant
    Filed: November 18, 2016
    Date of Patent: March 19, 2019
    Assignee: Servomex Group Limited
    Inventors: Bahram Alizadeh, James Hobby, Martin Lopez, Ian Gaskin
  • Patent number: 10082456
    Abstract: The present invention includes: an excitation light source; a probe light source; a filter that mutually multiplexes a probe light emitted from the probe light source and an excitation light emitted from the excitation light source to a same optical axis; a condenser lens that focuses the excitation light and the probe light; a sample cell that stores a sample; a reflection member that is disposed on an inner wall of the sample cell and reflects the probe light; and a detector that detects the probe light reflected at the reflection member.
    Type: Grant
    Filed: December 8, 2015
    Date of Patent: September 25, 2018
    Assignee: Hitachi, Ltd.
    Inventors: Jiro Hashizume, Kei Takenaka, Takanori Aono
  • Patent number: 9649631
    Abstract: Aspects of the disclosure provide a microfluidic chip. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, and a second domain for electrophoretic separation. The first domain includes at least a first reaction reservoir designated for PCR amplification based on a first sample, and a second reaction reservoir designated for PCR amplification based on a second sample. The second domain includes at least a first separation unit coupled to the first reaction reservoir to received first amplified DNA fragments based on the first sample, and a second separation unit coupled to the second reaction reservoir to received second amplified DNA fragments based on the second sample. The first separation unit is configured to perform electrophoretic separation for the first amplified DNA fragments, and the second separation unit is configured to perform electrophoretic separation for the second amplified DNA fragments.
    Type: Grant
    Filed: March 4, 2011
    Date of Patent: May 16, 2017
    Assignee: LEIDOS INNOVATIONS TECHNOLOGY, INC.
    Inventors: Joan M Bienvenue, James P Landers, Orion N Scott
  • Patent number: 9550215
    Abstract: A method and a system are provided for detecting particles moving through a detection region or regions for facilitating or processing a sample having one or more particles flowing through the detection region. The particle detection system may include an optically detectable pattern associated with a detection region. The optically detectable pattern may be configured to receive a particle optical signal and produce a patterned optical signal. The detection system may further include a detector configured to analyze the patterned optical signal to determine both a particle characteristic based on a property of the particle optical signal and a particle parameter based on a property of the optically detectable pattern.
    Type: Grant
    Filed: May 19, 2014
    Date of Patent: January 24, 2017
    Assignee: CYTONOME/ST, LLC
    Inventors: Manish Deshpande, John R. Gilbert
  • Patent number: 9322780
    Abstract: A method for wavelength-resolving and high spatial resolution fluorescence microscopy in which fluorescence labels in a sample are repeatedly excited to emit fluorescence radiation and frames including images of isolated labels are produced with a microscope. The positions of the images of the isolated fluorescing labels are localized with a localization precision exceeding the optical resolution of the imaging beam path of the microscope. The imaging beam path of the microscope has a diffractive element which, during the imaging, diffracts the image of the sample comprising the isolated fluorescing labels into a first diffraction order so that each frame contains the first diffraction order images of the isolated fluorescing labels. A parameter of the first diffraction order images of the isolated fluorescing labels is evaluated and an indication of the wavelength of the isolated fluorescing labels is derived from this evaluated parameter.
    Type: Grant
    Filed: February 22, 2013
    Date of Patent: April 26, 2016
    Assignee: Carl Zeiss Microscopy GmbH
    Inventors: Thomas Kalkbrenner, Ralf Wolleschensky
  • Patent number: 9091803
    Abstract: An optical apparatus for delivering to a flow-cell of a flow-cytometer a plurality of beams of laser radiation each thereof having a different wavelength. The apparatus includes a dispersion compensation-prism and a plurality of directing-prisms equal in number to the number of laser-beam. The directing-prisms are arranged to direct the laser radiation beams directly therethrough into the dispersion compensation-prism as converging fan of beams in a first plane. The beams are transmitted by the compensation-prism as a converging fan of beams intersecting then proceeding as a diverging fan of beams in the first plane. A spherical focusing lens is arranged cooperative with a cylindrical lens for focusing the plurality of laser-beams as a plurality of spaced apart elongated focal spots in a plane in which the cytometer flow-cell is located.
    Type: Grant
    Filed: July 15, 2014
    Date of Patent: July 28, 2015
    Assignee: Coherent, Inc.
    Inventors: Lei Meng, Jordan Wesley Crist, Michele Wayne Winz, James Schloss
  • Patent number: 8702942
    Abstract: In one general aspect, an electrophoretic measurement method is disclosed that includes providing a vessel that holds a dispersant, providing a first electrode immersed in the dispersant, and providing a second electrode immersed in the dispersant. A sample is placed at a location within the dispersant between the first and second electrodes with the sample being separated from the electrodes, an alternating electric field is applied across the electrodes, and the sample is illuminated with temporally coherent light. A frequency shift is detected in light from the step of illuminating that has interacted with the sample during the step of applying an alternating electric field, and a property of the sample is derived based on results of the step of detecting.
    Type: Grant
    Filed: December 17, 2010
    Date of Patent: April 22, 2014
    Assignee: Malvern Instruments, Ltd.
    Inventors: Jason Cecil William Corbett, Malcolm Connah, Kevin Mattison
  • Patent number: 8697004
    Abstract: The present teachings provide a device including a memory. According to various embodiments, the memory is readable, writable, and rewritable. The present teachings further provide processing stations, e.g., for carrying out electrophoresis, per, genetic analysis, sample preparation, and/or sample cleanup, etc., that are capable of reading from and/or writing/rewriting to such memory.
    Type: Grant
    Filed: May 19, 2010
    Date of Patent: April 15, 2014
    Assignee: Applied Biosystems, LLC
    Inventor: Jeffery D. Frazier
  • Patent number: 8625094
    Abstract: An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: January 7, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Mark Oldham, Eric Norman, Richard Reel, Janice Shigeura
  • Patent number: 8597485
    Abstract: An apparatus for aligning a capillary column with one or more excitation fibers and with one or more optical lens elements for Capillary Electrophoresis. The apparatus includes two identical blocks having a plurality of grooves for positioning and aligning the capillary column with the one or more excitation fibers, and a plurality of lens seats for optically coupling the lens element with the capillary column. Each block includes a male and female part for mating the two identical blocks together.
    Type: Grant
    Filed: April 5, 2010
    Date of Patent: December 3, 2013
    Assignee: Qiagen Sciences, LLC
    Inventors: Varouj Amirkhanian, Paul Mooney
  • Patent number: 8537356
    Abstract: Provided herein are new methods and apparatus for quantitative measurement and analysis of particles, including new apparatus systems to process and detect nanoparticles in suspension. By focusing a laser beam at the center of a reservoir, nanoparticles are concentrated by optical energy, and fluorescent intensity at the focal point of the laser is measured to quantify particle concentration in the reservoir. The techniques may be applied to the analysis of suspensions of nanoparticles, including natural particles (e.g., microorganisms including whole viruses, bacteria, animal cells, and proteins) and synthetic particles (e.g., colloidal latexes, paints, pigments, and metallic or semiconductor nanoparticles) for medical and industrial applications, among others.
    Type: Grant
    Filed: March 2, 2009
    Date of Patent: September 17, 2013
    Assignee: Lehigh University
    Inventors: H. Daniel Ou-Yang, Xuanhong Cheng
  • Patent number: 8525980
    Abstract: This invention provides a body fluid detection method by using surface enhanced Raman spectroscopy. In this method, some biological macromolecules in body fluid samples could be separated with membrane electrophoresis technique firstly. Next, samples are cut off along with the substrates and touched with glacial acetic acid. Transparent colloid formed while incubating. Then add enhancing substrates and continue to incubate and stir. When solid impurities precipitated, stop incubating and stand for layering. In the end, take upper layer resulted to be tested using SERS detection method and build SERS database. This invention successfully eliminated disturbance of other complex components on the SERS detection of protein, DNA and RNA. High quality SERS spectrum obtained is beneficial to the analysis and process of SERS spectrum. Thus body fluid can be differentiated by comparing body fluid SERS spectrum belonging to the healthy people and patients.
    Type: Grant
    Filed: June 21, 2010
    Date of Patent: September 3, 2013
    Inventors: Juqiang Lin, Rong Chen, Shangyuan Feng, Yongzeng Li, Guannan Chen, Zufang Huang, Min Cheng, Yun Yu, Haishan Zeng
  • Patent number: 8525991
    Abstract: A method is disclosed for measurement of the electrophoretic mobility of particles in solution. A sample is placed in a cell containing two electrodes that apply an alternating electric field. A monochromatic light beam passes through the sample. Light scattered by the particles, along with the unscattered beam, is collected and collimated as it exits the cell. This beam is combined in free space with a phase modulated reference beam. The interference forms a frequency modulated speckle pattern, which is detected by a photodetector array. Each array element collects a narrow range of well-defined scattering angles. The signal from each is demodulated to provide a first-principle measurement of the electrophoretic mobility of the scattering particles.
    Type: Grant
    Filed: March 1, 2013
    Date of Patent: September 3, 2013
    Assignee: Wyatt Technology Corporation
    Inventors: Hung-Te Hsieh, Steven P. Trainoff
  • Publication number: 20130222802
    Abstract: Systems, devices, and methods are presented that facilitate electronic manipulation and detection of submicron particles. A particle manipulation device contains a plurality of electrodes formed on an active semiconductor layer of an integrated circuit chip, where the electrodes and gap spacing between adjacent electrodes is submicron in size. The chip is oriented with its substrate face up, and at least a portion of the substrate is removed from the chip so the electrodes are in close proximity to a fluid chamber(s) placed over the chip, to facilitate manipulation of particles, contained in a buffer solution in the fluid chamber(s), to form a defined pattern. Innovative macro-scale optical detection is employed to detect the submicron particles, where a light beam is applied to the defined pattern, and interaction of the defined pattern with the light beam is detected and evaluated to facilitate detecting the particles.
    Type: Application
    Filed: August 28, 2012
    Publication date: August 29, 2013
    Applicant: University of Pittsburgh - Of the Commonwealth System of Higher Education
    Inventors: Steven P. Levitan, Samuel J. Dickerson, Donald M. Chiarulli
  • Patent number: 8467055
    Abstract: An optical measuring device includes: a light applying section configured to apply laser light to a sample flowing in a channel; and a fluorescence detecting section configured to detect fluorescence generated from the sample irradiated with the laser light; the fluorescence detecting section including a multichannel photomultiplier tube having a plurality of detection channels capable of simultaneously detecting a plurality of light beams, a light separator configured to separate the fluorescence according to wavelengths to provide the plurality of light beams, the light separator being provided by a transmission grating or a prism, and a telecentric condenser lens configured to receive the plurality of light beams from the light separator and direct the plurality of light beams toward the plurality of detection channels of the multichannel photomultiplier tube so that the optical axes of the plurality of light beams are parallel to each other.
    Type: Grant
    Filed: October 15, 2009
    Date of Patent: June 18, 2013
    Assignee: Sony Corporation
    Inventors: Shingo Imanishi, Takeo Arai, Suguro Dowaki
  • Patent number: 8441638
    Abstract: A method and apparatus are disclosed for measurement of the electrophoretic mobility of particles in solution. A sample is placed in a cell containing two electrodes that apply an alternating electric field. A monochromatic light beam passes through the sample. Light scattered by the particles, along with the unscattered beam, is collected and collimated as it exits the cell. This beam is combined in free space with a phase modulated reference beam. The interference forms a frequency modulated speckle pattern, which is detected by a photodetector array. Each array element collects a narrow range of well-defined scattering angles. The signal from each is demodulated to provide a measurement of the electrophoretic mobility of the scattering particles.
    Type: Grant
    Filed: May 18, 2010
    Date of Patent: May 14, 2013
    Assignee: Wyatt Technology Corporation
    Inventors: Hung-Te Hsieh, Steven P. Trainoff
  • Patent number: 8404092
    Abstract: The invention is a high-throughput multi-capillary system utilizing a 2-dimensional mask onto which the detection windows of the capillaries are lined up in alternating columns and rows. The image of the capillaries is projected onto a 2-dimensional detection system, such as a charge-coupled-detector (CCD) array. Each row and column of the capillary detection windows is imaged in a separate area of the CCD array, which results in reduced cross-talk between capillaries.
    Type: Grant
    Filed: March 26, 2012
    Date of Patent: March 26, 2013
    Assignee: Advanced Analytical Technologies, Inc.
    Inventors: Bruce Boeke, Mark McClure, Wei Wei, Ho-Ming Pang
  • Patent number: 8390814
    Abstract: This disclosure relates generally to a sampling device, and more particularly, a sampling device that facilitates spectroscopic measurements with a variable path length and the necessary software controlled algorithms and methods for such a device.
    Type: Grant
    Filed: September 9, 2011
    Date of Patent: March 5, 2013
    Assignee: C Technologies
    Inventors: Mark Salerno, I-Tsung Shih, Craig Harrison
  • Patent number: 8384898
    Abstract: An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
    Type: Grant
    Filed: April 9, 2012
    Date of Patent: February 26, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Mark Oldham, Eric Nordman, Richard Reel, Janice Shigeura
  • Patent number: 8384899
    Abstract: An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
    Type: Grant
    Filed: April 9, 2012
    Date of Patent: February 26, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Mark Oldham, Eric Nordman, Richard Reel, Janice Shigeura
  • Patent number: 8368893
    Abstract: An optical apparatus and method comprising a light source, an array detector for area imaging and an optical cell assembly. The optical cell assembly comprises a chamber which is arranged to receive a sample of a material including an analyte, a fluid inlet and a fluid outlet coupled to the chamber. A fluid dissolution medium stream passes through the chamber such that the sample can dissolve into the dissolution medium. The chamber is in at least one light path created between the light source and the array detector. The array detector comprises a two dimensional array of detector locations arranged to provide an output signal indicative of the light absorbance of the analyte within the chamber such that the output of the array detector is indicative of the concentration profile of the analyte near the surface of the sample.
    Type: Grant
    Filed: December 1, 2009
    Date of Patent: February 5, 2013
    Assignee: Paraytec Limited
    Inventors: James Hershell Lenke, Kevin James Moon, David Murray Goodall
  • Patent number: 8268249
    Abstract: An analytical cell including a lightguide with a plurality of conduits filled with a migration medium. The medium, the lightguide and a surrounding medium have refractive indices selected such that light entering the lightguide is internally reflected within the lightguide to provide substantially uniform illumination of the conduits.
    Type: Grant
    Filed: November 16, 2006
    Date of Patent: September 18, 2012
    Assignees: 3M Innovative Properties Company, Life Technologies Corporation
    Inventors: Larry J. Carson, Joel R. Dufresne, Patrick R. Fleming, Michael C. Lea, Nicholas A. Lee, John Shigeura
  • Patent number: 8262888
    Abstract: There is provided a capillary electrophoresis apparatus wherein coupling efficiency of exciting irradiation light to a capillary array does not decline even if any one capillary array of two capillary arrays is removed. Light emission generated by capillaries of each capillary array of 2n (n is a positive integer) capillary arrays radiating exciting light from one side is detected by one optical detection system.
    Type: Grant
    Filed: March 4, 2008
    Date of Patent: September 11, 2012
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Ryoji Inaba, Motohiro Yamazaki, Tomohiro Shoji, Takeshi Ohura, Takashi Gomi
  • Publication number: 20120212740
    Abstract: An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
    Type: Application
    Filed: April 9, 2012
    Publication date: August 23, 2012
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Mark E. OLDHAM, Eric S. NORDMAN, Richard T. REEL, John S. SHIGEURA, Janice G. SHIGEURA
  • Patent number: 8246806
    Abstract: An electrophoresis apparatus includes a multi-capillary array having a liquid or solid disposed between the capillaries of the array. The liquid or solid exhibits a refractive index higher than that of air and less than that of water and reduces the amount of laser beams scattered by the capillaries. Also provided are methods of adjusting refracted and reflected excitation light beams passing through capillaries of a multi-capillary array, to reduce loss of intensity of the laser beams and increase irradiation of respective samples disposed in the capillaries.
    Type: Grant
    Filed: March 6, 2007
    Date of Patent: August 21, 2012
    Assignees: Applera Corporation, Hitachi, Ltd.
    Inventors: Ryoji Inaba, Tomohiro Shoji, Shozo Kasai, Masaya Kojima, Yasushi Shimizu, Seiichi Ugai, Eric S. Nordman
  • Patent number: 8246803
    Abstract: An object of the present invention is to provide a capillary electrophoresis apparatus in which simultaneity can be ensured between sensitivity and data acquisition to decrease a pull-up signal while spectral data acquisition is eliminated in each capillary exchange. The invention relates to a capillary electrophoresis apparatus characterized in that a multi-bandpass filter is provided in an optical detection system. In one aspect of the invention, a signal detection area of a two-dimensional detector is divided into plural regions corresponding to wavelength transmission regions of the multi-bandpass filter. An integrated value of the fluorescence spectrum signal is determined in the region including a fluorescence spectrum peak of an analysis sample in the plural regions. The analysis is performed with the integrated value.
    Type: Grant
    Filed: January 18, 2007
    Date of Patent: August 21, 2012
    Assignee: Hitachi High Technologies Corporation
    Inventors: Motohiro Yamazaki, Ryoji Inaba, Satoshi Takahashi, Shigeru Matsui
  • Patent number: 8233735
    Abstract: Fluorescently marked targets bind to a substrate 230 synthesized with polymer sequences at known locations. The targets are detected by exposing selected regions of the substrate 230 to light from a light source 100 and detecting the photons from the light fluoresced therefrom, and repeating the steps of exposure and detection until the substrate 230 is completely examined. The resulting data can be used to determine binding affinity of the targets to specific polymer sequences.
    Type: Grant
    Filed: September 17, 2008
    Date of Patent: July 31, 2012
    Assignee: Affymetrix, Inc.
    Inventors: David Stern, Peter Fiekowsky
  • Patent number: 8169610
    Abstract: An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
    Type: Grant
    Filed: June 6, 2011
    Date of Patent: May 1, 2012
    Assignee: Life Technologies Corporation
    Inventors: Mark Oldham, Eric Nordman, Richard Reel, John Shigeura, Janice Shigeura, legal representative
  • Patent number: 8163151
    Abstract: Methods of analyzing the electrophoretic mobility distribution of von Willebrand factor (vWF) multimers include providing a sample medium comprising a plurality vWF multimers. The vWF multimers are electrophoretically separated by electrohoretic mobility in the sample medium by subjecting said sample medium to an electric field to provide separated vWF multimers. The separated vWF multimers in the sample medium are exposed to a light source to produce scattered light. The scattered light is detected, and the electrophoretic mobility distribution of the separated vWF multimers is determined from the detected scattered light.
    Type: Grant
    Filed: July 21, 2010
    Date of Patent: April 24, 2012
    Assignee: The University of North Carolina at Chapel Hill
    Inventor: Don A. Gabriel
  • Patent number: 8124943
    Abstract: A method for altering fluorescent emissions of particles includes setting the particles in motion and exposing the moving particles to light such that fluorescent intensities of the particles are lessened isotropically and substantially simultaneously. Another method includes measuring fluorescent emissions of particles, determining the measured fluorescent emissions do not collectively fit within a first predetermined range of fluorescent values, and exposing the particles to one or more incidents of light that are configured to cooperatively alter the fluorescent emissions of the particles to be within a second predetermined range of fluorescent values. An embodiment of an apparatus includes a vessel configured to contain a plurality of particles and a means for setting the particles in motion. The apparatus further includes an illumination subsystem configured to direct light toward the vessel and at a spectral window (i.e.
    Type: Grant
    Filed: April 5, 2007
    Date of Patent: February 28, 2012
    Inventors: Ananda G. Lugade, Bruce J. C. Bernard, Kurt D. Hoffacker
  • Patent number: 8114349
    Abstract: A reusable bio-analysis cartridge that includes a built-in mechanism to track various parameters and usage data. The tracking mechanism includes a non-volatile rewritable memory “smart-key” that is associated with the cartridge to provide automatic tracking dedicated to the cartridge. The key may be physically coupled to the cartridge with good high voltage insulating properties. When the cartridge is used in an instrument, the associated key is inserted into an I/O interface to communicate with the instrument. The instrument may be configured to “authenticate” the cartridge and conduct an integrity check to determine if the particular cartridge has the correct properties (e.g., gel-chemistry, serial no., Patient I.D.) for the particular sample analysis to be conducted. Further, the instrument may communicate/record information concerning usage of the cartridge (e.g., usage history, test parameters, and perhaps test results).
    Type: Grant
    Filed: December 23, 2004
    Date of Patent: February 14, 2012
    Assignee: Qiagen Sciences, LLC
    Inventors: Varouj Amirkhanian, Ming-Sun Liu, Paul Mooney
  • Patent number: 8077314
    Abstract: To measure a characteristic of a multimode optical fiber, a light pulse source produces a light pulse for transmission into the multimode optical fiber. A spatial filter passes a portion of Brillouin backscattered light from the multimode optical fiber that is responsive to the light pulse. Optical detection equipment detects the portion of the Brillouin backscattered light passed by the spatial filter.
    Type: Grant
    Filed: September 30, 2008
    Date of Patent: December 13, 2011
    Assignee: Schlumberger Technology Corporation
    Inventors: Dylan Davies, Arthur H. Hartog, Graeme Hilton, Gareth P. Lees
  • Patent number: 8043493
    Abstract: An electrophoresis apparatus includes a multi-capillary array having a liquid or solid disposed between the capillaries of the array. The liquid or solid exhibits a refractive index higher than that of air and less than that of water and reduces the amount of laser beams scattered by the capillaries. Also provided are methods of adjusting refracted and reflected excitation light beams passing through capillaries of a multi-capillary array, to reduce loss of intensity of the laser beams and increase irradiation of respective samples disposed in the capillaries.
    Type: Grant
    Filed: March 6, 2007
    Date of Patent: October 25, 2011
    Assignees: Hitachi, Ltd., Applera Corporation
    Inventors: Ryoji Inaba, Tomohiro Shoji, Shozo Kasai, Masaya Kojima, Yasushi Shimizu, Seiichi Ugai
  • Patent number: 8018593
    Abstract: The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.
    Type: Grant
    Filed: April 4, 2008
    Date of Patent: September 13, 2011
    Assignee: NetBio, Inc.
    Inventors: Eugene Tan, Heung Chuan Lam, Valery Leonidovich Bogdanov, Gregory John Kellogg, John A. Wright, Ulrich Hans Thomann, Richard F. Selden
  • Patent number: 8012327
    Abstract: An object of the present invention is to provide a capillary electrophoresis apparatus in which simultaneity can be ensured between sensitivity and data acquisition to decrease a pull-up signal while spectral data acquisition is eliminated in each capillary exchange. The invention relates to a capillary electrophoresis apparatus in which capillary position shift is detected in each capillary exchange by detecting a capillary position. A capillary position measuring light source is provided in the capillary electrophoresis apparatus of the invention. The capillary is irradiated with light emitted from the capillary position measuring light source, a capillary image is detected with a two-dimensional detector, and thereby a position deviation of the capillary is determined. On the basis of the position deviation of the capillary, a data acquisition area is set in the two-dimensional detector, or a reference fluorescent light spectrum determined from the capillary at the standard position is corrected.
    Type: Grant
    Filed: January 16, 2007
    Date of Patent: September 6, 2011
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Motohiro Yamazaki, Ryoji Inaba, Satoshi Takahashi, Isao Haraura, Tomoyuki Sakai
  • Patent number: 7978326
    Abstract: An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
    Type: Grant
    Filed: December 3, 2009
    Date of Patent: July 12, 2011
    Assignee: Applied Biosystems, LLC
    Inventors: Mark F. Oldham, Eric S. Nordman, Richard T. Reel, John S. Shigeura, Janice G. Shigeura, legal representative
  • Patent number: 7929138
    Abstract: An ambient atmosphere glow discharge spectrometer is disclosed having a capillary, two electrodes and a means for recording the atomic emissions.
    Type: Grant
    Filed: December 17, 2008
    Date of Patent: April 19, 2011
    Assignee: The United States of America as represented by the United States Department of Energy
    Inventors: Michael R. Webb, Gary M. Hieftje, Francisco Andrade
  • Publication number: 20100231909
    Abstract: An apparatus and method for determining characteristics of particles, by measuring characteristics which are related to the velocity of the particles. Particle size distribution is determined from motion of the particles in an acceleration field, or from Brownian motion of the particles. Zeta potential and particle mobility are determined by measuring velocity related characteristics of charged particles in an electric field. Particle velocity characteristics are determined by measuring dynamic properties of light, which is scattered by the particles. A light source illuminates the particles. Scattered light, from the particles, is mixed with light, from the light source, onto at least one light detector. The detector produces a signal, which is indicative of velocity related characteristics of the particles. The velocity characteristics are also determined by measuring light scattered from particles moving through an illumination pattern, with a periodic intensity structure.
    Type: Application
    Filed: March 30, 2010
    Publication date: September 16, 2010
    Inventor: Michael Trainer
  • Patent number: 7780831
    Abstract: Methods of analyzing the electrophoretic mobility distribution of von Willebrand factor (vWF) multimers include providing a sample medium comprising a plurality vWF multimers. The vWF multimers are electrophoretically separated by electrohoretic mobility in the sample medium by subjecting said sample medium to an electric field to provide separated vWF multimers. The separated vWF multimers in the sample medium are exposed to a light source to produce scattered light. The scattered light is detected, and the electrophoretic mobility distribution of the separated vWF multimers is determined from the detected scattered light.
    Type: Grant
    Filed: July 7, 2004
    Date of Patent: August 24, 2010
    Assignee: The University of North Carolina at Chapel Hill
    Inventor: Don A. Gabriel
  • Patent number: 7767468
    Abstract: Improved methods for the separation, isolation, enrichment, or detection of target molecules, such as nucleic acids and proteins, within dilute or heterogeneous samples, such as bodily fluids, excreta or tissue samples, are disclosed. The methods include repetitively and rapidly passing a sample across at least one region of a conduit in which at least one region includes a binding partner specific for the target molecule. In certain methods, at least one other region includes binding partners specific for non-target molecules. The sample may be passed over the binding partner in the same direction if the conduit is a loop or in an antiparallel direction (i.e., back and forth over the binding partner). In an embodiment, the sample is electrophoresed through or over an electrophoretic medium, in which at least one region includes a binding partner for the target molecule. The invention also provides apparatus and sample preparation systems adapted for use in the methods.
    Type: Grant
    Filed: November 5, 2004
    Date of Patent: August 3, 2010
    Assignee: EXACT Sciences Corporation
    Inventors: Anthony P. Shuber, Duncan Whitney
  • Publication number: 20100103416
    Abstract: An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
    Type: Application
    Filed: December 3, 2009
    Publication date: April 29, 2010
    Applicant: Applied Biosystems, LLC
    Inventors: Mark F. OLDHAM, Eric S. Nordman, Richard T. Reel, Janice S. Shigeura
  • Patent number: 7691247
    Abstract: An apparatus for aligning a capillary column with one or more excitation fibers and with one or more optical lens elements for Capillary Electrophoresis. The apparatus includes two identical blocks having a plurality of grooves for positioning and aligning the capillary column with the one or more excitation fibers, and a plurality of lens seats for optically coupling the lens element with the capillary column. Each block includes a male and female part for mating the two identical blocks together.
    Type: Grant
    Filed: June 13, 2005
    Date of Patent: April 6, 2010
    Assignee: Biocal Technology, Inc.
    Inventors: Varouj Amirkhanian, Paul Mooney
  • Patent number: 7636160
    Abstract: An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
    Type: Grant
    Filed: January 26, 2009
    Date of Patent: December 22, 2009
    Assignee: Applied Biosystems, LLC
    Inventors: Mark F. Oldham, Eric S. Nordman, Richard T. Reel, John S. Shigeura, Janice G. Shigeura, legal representative
  • Patent number: 7636159
    Abstract: An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
    Type: Grant
    Filed: September 17, 2008
    Date of Patent: December 22, 2009
    Assignee: Applied Biosystems, LLC
    Inventors: Mark F. Oldham, Eric S. Nordman, Richard T. Reel, John S. Shigeura, Janice G. Shigeura, legal representative
  • Patent number: 7545496
    Abstract: A support structure, such as an optical disc, is designed having surface structures formed on one or more surfaces of the support to enable formation of evanescent fields in a medium adjacent to the surface structures and allow for detection of optically-active substances within the evanescent fields in the medium. The surface structures may be formed in one or more areas on the surface of the support. The surface structures may have inclined surfaces that are inclined with respect to a general plane of the support surface, and form wedge structures or symmetric pyramidal structures, for example.
    Type: Grant
    Filed: June 14, 2004
    Date of Patent: June 9, 2009
    Assignee: Koninklijke Philips Electronics N.V.
    Inventors: Menno Willem Jose Prins, Johannes Joseph Hubertina Barbara Schleipen
  • Publication number: 20090135421
    Abstract: An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
    Type: Application
    Filed: January 26, 2009
    Publication date: May 28, 2009
    Applicant: Applera Corporation
    Inventors: Mark F. Oldham, Eric S. Nordman, Richard T. Reel, John S. Shigeura, Janice G. Shigeura
  • Publication number: 20090128809
    Abstract: The method of the present invention generates a regularly lined electric field inside a container 1 retaining a sample formed by dispersing particle groups in a liquid by a voltage being applied to an electrode pair 2 provided in the container 1, generates a diffraction grating by a density distribution of the particle groups in the sample inside the container 1, and when acquiring a diffusion coefficient of particles from a temporal variation of intensity in a disappearing process of a diffracted light obtained by irradiating a beam of light to the diffraction grating generated by the density distribution of the particle groups, performs a particle size analysis of the particle groups by using an approximate analysis expression of a diffracted light attenuation, I(t)=?exp[?2q2Dt] which uses q=2?/? defined by a particle concentration modulation period ? in the density distribution diffraction grating of the particle groups, and the Einstein-Stokes relation.
    Type: Application
    Filed: February 28, 2006
    Publication date: May 21, 2009
    Inventor: Naoji Moriya
  • Publication number: 20090116028
    Abstract: An optical system for analyzing light from a plurality of samples is provided. The optical system includes a plurality of holders adapted to have samples located therein, a collection lens, a transmission grating, and a reimaging lens. The collection lens is configured to receive and substantially collimate light from the samples. The transmission grating is configured to spectrally disperse the substantially collimated light from the collection lens. The reimaging lens is configured to receive the light from the light dispersing element and direct the light onto a light detection device. A method of optically analyzing at least one sample is also provided.
    Type: Application
    Filed: January 13, 2009
    Publication date: May 7, 2009
    Applicant: Life Technologies Corporation
    Inventor: Richard T. Reel
  • Patent number: 7528398
    Abstract: An imaging section is secured to a casing, and an object is supported on an object support section, which is located within the casing. The object support section is moved within the casing in order for an imaging distance between the imaging section and the object, which has been supported on the object support section, to be altered. Excitation light is irradiated to the object, which has been supported on the object support section, with an excitation light source, which is secured to the object support section. A distribution of illuminances of the excitation light on the object is thus prevented from fluctuating to a fluctuation of the imaging distance.
    Type: Grant
    Filed: March 28, 2003
    Date of Patent: May 5, 2009
    Assignee: FUJIFILM Corporation
    Inventor: Seishi Ikami
  • Patent number: 7518727
    Abstract: A system for detecting electromagnetic radiation from samples comprising: a plurality of sample volumes, each of said sample volumes confined within a portion of a capillary column; a plurality of electromagnetic radiation sources; a mirror for receiving electromagnetic radiation from the electromagnetic radiation sources and for reflecting the electromagnetic radiation to the sample volumes; a scanner attached to the mirror; a parabolic reflector for collecting sample electromagnetic radiation from the sample volumes, the sample electromagnetic radiation being generated as a result of interaction of the reflected electromagnetic radiation with the sample volumes; a plurality of filters for filtering the sample electromagnetic radiation; and a plurality of detectors for detecting sample electromagnetic radiation from the sample volumes, each of the detectors being configured to receive sample electromagnetic radiation that has passed through a corresponding one of the plurality of filters and generate a signa
    Type: Grant
    Filed: February 28, 2007
    Date of Patent: April 14, 2009
    Assignee: Beckman Coulter, Inc.
    Inventors: Stephen L. Pentoney, Jr., David L. Yang