Abstract: Systems and methods for performing a photoluminescence analysis on a medium such as the fundus of a patient's eye are provided. An imaging device, a spectral analyser and an excitation light source are provided. An optical assembly defines an imaging light path between the patient's eye and the imaging device and a spectral analysis light path between an analysis spot on the patient's eye and the spectral analyser. The excitation light source generates an excitation light beam comprising an excitation wavelength selected to excite components present in the patient's eye for the generation of fluorescent light at a photoluminescence wavelength, and is optically coupled to the spectral analysis light path for projecting the excitation light beam on the analysis spot. An optical filter is coupled to the spectral analyser, and has a low light transmissivity at the excitation wavelength and a high light transmissivity at the photoluminescence wavelength.

Abstract: In an embodiment an apparatus includes at least one optoelectronic laser configured to provide excitation radiation to a sample, the excitation radiation being generated by an electric current flowing through the at least one optoelectronic laser, a transistor configured to modulate the electric current flowing through the at least one optoelectronic laser in order to switch on and off generation of the excitation radiation and a spectrometer configured to analyze Raman light scattered from the sample in response to exposing the sample to the excitation radiation, wherein the Raman light includes one or more spectral components, wherein the spectrometer includes a diffraction element configured to split the Raman light into the spectral components, and wherein the diffraction element includes at least a photonic crystal or a plasmonic Fabry Perot filter.

Abstract: A system for virus detection, and related method, employ a fluorescence detection device to capture an image of processed samples irradiated by fluorescence excitation light. A system includes a fluorescence detection device and a computing device. The fluorescence detection device includes an image capture device that captures an image of fluorescence emitted by samples in response to incidence of the excitation light onto the samples. The computing device processes the image to determine one or more detection results for the presence of one or more viruses in the samples.

Abstract: Systems, methods, devices and software for operating particle sampling devices in a user-restrictive manner include a tag and a particle sampling device. The device includes a tag reader and a processor in communication with the tag reader. The processor: receives device configuration data and reads operational and/or user data from the tag having that data encoded thereon. Based on the data read from the tag, the processor may either grant or deny access to a user for performing device operations. Alternatively, for a headless particle sampling device configured for minimal user interaction during operation, the device is removably attached to a supporting structure proximate the tag positioned in or on the supporting structure. In the headless configuration, the processor reads device configuration parameters including network communication information from the tag following device power up.

Abstract: Disclosed are an online vacuum degree detection system and method based on single-fiber laser-induced breakdown spectroscopy. In the system, a laser device generates laser that excites the laser through fiber induced breakdown spectroscopy; a fiber coupler couples and injects the laser; a single fiber is connected to the fiber coupler to transmit the laser; one end of an LIBS probe is connected to the single fiber, and the other end of the LIBS probe extends into a vacuum arc-extinguishing chamber; the laser is induced by the LIBS probe to generate plasma, and the plasma is subjected to self-emission imaging and enters the fiber coupler via the LIBS probe; a dichroscope is arranged on the fiber coupler to separate the laser from the plasma; a processor is connected to a camera and a spectrometer.

Abstract: A device for spectral analysis including a seat for holding therein a compound specimen; a light source for illuminating the compound specimen with a spectrum of light; and a detector configured for detecting light transmitted through or reflected from the biological sample, the detector including a pixel array having a plurality of pixels each of which being configured to detect intensity of one wavelength within the spectrum such that the pixel array obtains a spectral signature of the biological sample including intensities of wavelengths within the spectrum.

Abstract: An apparatus and method of inline measurement of low-concentration hydrocarbons overlaps fluorescence, scatter and absorption spectroscopy devices so as to measure scatter and absorption of fluorescing oil and the excited fluorescence itself. The apparatus includes a fitting, an input port, an output port, and a sapphire tube having a hollow interior in fluid connection with the input port and the output port. Flow medium passes through the input port, the sapphire tube, and the output port. The apparatus also includes a light emitter, a first detector, and a second detector. The light emitter can include a lens, an absorption and scatter wavelength emitter, and a fluorescence wavelength emitter. An incident absorption and scatter beam and an incident fluorescence beam from the light emitter and parallel so as to determine free hydrocarbon, dissolved hydrocarbons, and solids in a sample within the sapphire tube.

Abstract: The invention relates to an apparatus for simultaneously analysing luminescence of a plurality of samples. The apparatus comprises a fibre-optic plate.

Abstract: The disclosed flow cytometer includes a wavelength division multiplexer (WDM). The WDM includes an extended light source providing light that forms an object, a collimating optical element that captures light from the extended light source and projects a magnified image of the object as a first light beam, and a first focusing optical element configured to focus the first light beam to a size smaller than the object of the extended light source to a first semiconductor detector. The disclosed flow cytometer further includes a composite microscope objective to direct light emitted by a particle in a flow channel in a viewing zone of the composite microscope to the extended light source, a fluidic system and a peristaltic pump configured to supply liquid sheath and liquid sample to the flow channel, and a laser diode system to illuminate the particle in the flow channel.

Abstract: A calibration reference for a multipass Raman analysis system, wherein a combination excitation and collection beam passes through a focal point F0 within a sample volume multiple times, is provided. The calibration reference includes a body of material having a known spectral response when illuminated by the combination excitation and collection beam. The size or shape of the body is selected or modified to keep the focal point at F0 within the body when the body is positioned within the sample volume for calibration purposes.

Abstract: There are provided infrared imaging systems and methods for imaging a sample with fluorescent markers. The system includes a light source configured to illuminate a sample-contacting surface. The light source includes first and second illumination modules, each configured to project a corresponding first and second infrared illumination beam towards a sample holder, the infrared illumination beams interacting at an imaging plane to define an illumination area having a rectangular and homogeneous power profile. The system also includes a control unit operatively connected to a motor assembly and to an optomechanical mechanism. The control unit is configured to superimpose the sample plane and the imaging plane at any of the multiple locations within the enclosure. The system includes a detector configured to receive light emitted by the fluorescent markers of the sample upon illumination of the same in the imaging plane when the sample plane is superimposed with the imaging plane.

Abstract: Methods, systems, and apparatus, including computer programs encoded on computer storage media, for a light-to-frequency sensor conversion. In some implementations, a first output waveform is generated from a light signal, a frequency of the first output waveform being based on an intensity of the light signal, including integrating the light signal across multiple clock cycles. In response to receiving a notification, a hold operation is performed to stop integrating the light signal for a period of time. In response to an end of the hold operation, a second output waveform is generated that includes integrating the intensity of the light signal starting from the end of the hold operation over a count of the multiple clock cycles that start after the end of the hold operation by a delay amount. The first output waveform and the second output waveform are summed to determine the intensity of the light signal.

Abstract: Disclosed are a differential dark-field confocal microscopic measurement apparatus and method based on a polarized vector light beam. The apparatus includes a vector polarized illumination light generation module, a light beam scanning illumination module and a differential dark-field confocal imaging module; a half wave plate and a vortex wave plate are regulated to generate radially polarized signal light and azimuthally polarized signal light respectively, and an acousto-optic modulator is controlled to modulate light beams into a pulse form, so that the radially polarized signal light and the azimuthally polarized signal light alternately illuminate during the same period, both of which have a time occupation ratio of 50%, separately.

Abstract: A method for scanning a sample in microscopy includes generating at least three illumination spots in order to form a spot pattern that contains at least two illumination spots having a first wavelength and an illumination spot having a second wavelength that differs from the first wavelength. At least one specified region of the sample is scanned by moving the spot pattern formed by the illumination spots along a first direction for generating scan lines, which are each associated with the illumination spots of the spot pattern, and by moving the spot pattern formed by the illumination spots along a second direction for generating scan lines respectively after the scan lines.

Abstract: An imaging apparatus for imaging a sample (7) comprises an array of electronically addressable pixels (6) wherein each pixel is arranged to support a surface plasmon resonance therein to generate an evanescent electromagnetic field. This field extends transversely from the pixel so as to be salient from the array at a first side of the array for illuminating the sample at said first side. A light source (15) is arranged to illuminate the array with excitation light therewith to generate said surface plasmon resonance. An optical detector (12A, 12B, 12C) is arranged at a second side of the array which is opposite to said first side of the array for detecting optical radiation returned from the array in response to illumination of the array by said excitation light. A processing unit (4) is arranged to associate the detected optical radiation with the address of the pixel or pixels within the array at which the surface plasmon resonance was generated.

Abstract: An apparatus for a polymerase chain reaction (PCR) diagnosis includes a transmitter that includes a multi-wavelength light source outputting a first light source signal and a second light source signal, which have different wavelengths, and that applies the first light source signal and the second light source signal to a DNA sample, a code generator that generates a first code signal and a second code signal corresponding to the first light source signal and the second light source signal, respectively, a delay controller that generates a first delay signal and a second delay signal corresponding to the first code signal and the second code signal, respectively, and a receiver that compares the first and second fluorescent signals emitted from the DNA sample with the first and second delay signals, and accumulatively integrates the first fluorescent signal or the second fluorescent signal based on the comparison result.

Abstract: A radiation carrier for carrying at least a radiation beam has, on a surface thereof, at least one excitation grating, for directing at least an excitation radiation beam directionally out of the radiation carrier, thereby illuminating a region of interest; and at least one structure for redirecting emission radiation emanating from the region of interest. Further a sensor is provided comprising at least one such radiation carrier and at least one detector, the structure being adapted for redirecting radiation from the region of interest into the at least one detector.

Abstract: Disclosed are an imaging system and an imaging method thereof. The system includes a light source, a light source modulator, an objective lens, a sensor array, a sensor read-write module and an imaging display module connected with the sensor read-write module. The light source modulator is configured for modulating the light source to a preset frequency; the objective lens is configured for imaging an optical signal reflected by a surface of a target object on the sensor array; the sensor array is configured for converting the received optical signal into an analog alternating current electrical signal; the sensor read-write module is configured for amplifying the analog alternating current electrical signal and extracting a target signal, converting the target signal into a digital signal and storing the digital signal; and the imaging display module is configured for restoring and displaying an image of the target object according to the digital signal.

Abstract: The present invention provides an apparatus for detecting photoluminescent light emitted from a sample, said apparatus (200; 300) comprising at least one light source (210; 310, 318), which is configured to emit light of a first and a second wavelength towards a sample comprising photoluminescent particles, wherein said first wavelength is an excitation wavelength for inducing photoluminescent light from said photoluminescent particles, and wherein said second wavelength is longer than said first wavelength and for gathering background noise information from said sample.

Abstract: Disclosed is a method for acquiring information of a target polypeptide, comprising: acquiring a diffusion time of a fluorescently labeled target polypeptide and a diffusion time of each of a plurality of fluorescently labeled reference polypeptides by fluorescence correlation spectroscopy or fluorescence cross-correlation spectroscopy, and acquiring information on size of the fluorescently labeled target polypeptide from the diffusion time of the fluorescently labeled target polypeptide with reference to the diffusion times of the plurality of fluorescently labeled reference polypeptides, wherein information on size of each of the plurality of fluorescently labeled reference polypeptides is known, and the sizes of the plurality of reference polypeptides are different from each other.

Abstract: A system comprising: at least one hardware processor; and a non-transitory computer-readable storage medium having stored thereon program instructions, the program instructions executable by the at least one hardware processor to: receive, as input, spectral image data representing spectral reflectance associated with at least one plant, calculate a first and second chlorophyll fluorescence indices in respective first and second wavelength bands, based, at least in part, on said spectral reflectance, and derive a quantum yield value with respect to said at least one plant, by: (i) dividing said first chlorophyll fluorescence index in a sum of said first and second chlorophyll fluorescence indices, and (ii) multiplying (i) by a vegetation index value associated with said at least one plant.

Abstract: Provided is a confocal sensor having a wider measuring range. A confocal sensor 1 comprises: a light source 10 that emits light at a plurality of wavelengths; a diffractive lens 130 that causes a chromatic aberration with respect to the light along an optical axis and focuses the light onto an object 200 without another intervening lens; a pinhole 120 through which reflected light passes, the reflected light being a portion of the light that was focused onto and reflected from the object 200 and focused by the diffractive lens 130; and a measuring unit 40 that measures the distance from the diffractive lens 130 to the object 200 on the basis of a wavelength of the reflected light. A distance L2 from the pinhole 120 to the diffractive lens 130 is variable.

Abstract: An imaging system is configured with an autofocus operation that refocuses multiple cameras of the imaging system on detected features of interest, instead of on the whole image of the sample. Focus measures are calculated on the detected features, and then aggregated to focus distances of actuator moving the camera array, the sample stage, or individual cameras based on a maximization of detected features to be in focus. After the refocus, the imaging system recaptures new images and analyzes features on the new images to generate statistical characterization of the sample based on the classification of the features.

Abstract: A system including a signal obtaining module and a controller is provided. The signal obtaining module includes: a receiver to which an emission light generated in the target by an excitation light is input; a receiving optical path that guides the emission light and a residual light, which is at least a part of the excitation light propagated forward, coaxially between the target and the receiver; a separator that separates the residual light from the receiving optical path to be routed to an image sensor; and an actuator for controlling an optical relative position between the target and the receiver. The controller includes a module that controls the actuator to maintain an optical alignment.

Abstract: A machine that automatically performs select tests on candles includes a pedestal onto which a candle is positioned. The machine also includes cameras, focused on the candle, to collect thermal emission and visual data resulting from a “candle burn”. The machine is used in a “candle burn” test method.

Abstract: An apparatus for analyzing a plant specimen is disclosed which includes a housing assembly adapted to be in i) an open configuration adapted to receive a plant specimen, and ii) a closed configuration wherein ambient light is controlled therein, a light source disposed in or coupled to the housing assembly, the light source adapted to shine light onto or through the plant specimen when the housing assembly is in the closed configuration, and a camera assembly coupled to the housing assembly, the camera assembly having an image sensor adapted to receive light from the plant specimen in i) a transmittance mode where light transmits through the plant specimen, or ii) a reflectance mode where light is reflected from the plant specimen, the image sensor adapted to thereby capture hyperspectral images of the plant specimen.

Abstract: An optical measurement system includes a light source, a spectroscopic detector, a reference sample, a switching mechanism that switches between a first optical path through which a sample to be measured is irradiated with light from the light source and light produced at the sample is guided to the spectroscopic detector and a second optical path through which the reference sample is irradiated with light from the light source and light produced at the reference sample is guided to the spectroscopic detector, and a processing unit that calculates, by performing correction processing based on change between a first detection result at first time and a second detection result at second time, a measurement value of the sample from a third detection result provided from the spectroscopic detector as a result of irradiation of the sample with light from the light source at third time temporally proximate to the second time.

Abstract: An apparatus and method of inline measurement of low-concentration hydrocarbons overlaps fluorescence, scatter and absorption spectroscopy devices so as to measure scatter and absorption of fluorescing oil and the excited fluorescence itself. The apparatus includes a fitting, an input port, an output port, and a sapphire tube having a hollow interior in fluid connection with the input port and the output port. Flow medium passes through the input port, the sapphire tube, and the output port. The apparatus also includes a light emitter, a first detector, and a second detector. The light emitter can include a lens, an absorption and scatter wavelength emitter, and a fluorescence wavelength emitter. An incident absorption and scatter beam and an incident fluorescence beam from the light emitter and parallel so as to determine free hydrocarbon, dissolved hydrocarbons, and solids in a sample within the sapphire tube.

Abstract: A fiber optic probe is provided with a distal sampling end, a proximal end, and light delivery and collection paths therethrough. The probe includes a window disposed at the distal sampling end of the fiber optic probe, the window having a distal end and a proximal end. A lens is disposed at the proximal end of the window, the lens having a distal end, a proximal end, and an aperture. A light delivery optical fiber is provided having a distal end and a proximal end, the distal end being received by the lens aperture. An optical isolator provided within the lens aperture to optically isolate the light delivery path and the light collection path. A collection optical fiber is provided in optical communication with the fiber collection filter. The probe may include a lens collection filter disposed between the window and the lens.

Abstract: In variants, a method for automated experimentation can include: determining experimental constraints, constructing a computational representation of the experiment, optimizing the computational representation subject to the experimental constraints, determining instructions for a laboratory robot based on the optimized computational representation, and/or any other suitable steps.

Abstract: Methods and systems for macromolecular sequencing of one or a small number of a particular macromolecule are provided. The methods and systems generally operate by flowing a sample containing the macromolecule between a first electrode and a second electrode separated by a small gap. On one side of the gap, the first electrode is electrically coupled to an electron source that produces electrons with a narrow distribution of energies. On the other side of the gap, the second electrode is electrically coupled to an electric current sensor that detects electric current flowing across the gap from the first electrode to the second electrode.

Abstract: An agricultural nutrient applicator includes a container and a nutrient distribution assembly operably coupled to the container to deliver a nutrient from the container. A spectroscopic reflectance crop sense system is provided that includes an optical window. A presentation assembly is mounted to the agricultural nutrient applicator and is configured to position live plants in a field proximate the optical window of the spectroscopic reflectance crop sense system as the agricultural nutrient applicator moves. A controller is coupled to the spectroscopic reflectance crop sense system and the nutrient distribution assembly. The controller is configured to obtain, from the spectroscopic reflectance crop sense system, information indicative of a measured nutrient level in the live plants and determine a remedial nutrient amount based on the measured nutrient level and a target nutrient level. The controller controls the nutrient distribution assembly based on the remedial amount.

Abstract: A microscopy system for simultaneously observing fluorescent and non-fluorescent regions of an object, and a method for operating the microscopy system are provided. The microscopy system includes a microscopy optical unit configured to image an object plane through an observation beam path onto an image plane, an observation filter arrangeable in the observation beam path, two light sources, one being provided for exciting a fluorescent dye in the object and another being provided for visualizing non-fluorescent regions of the object, and a controller to control the light sources individually. With a suitable configuration of the observation filter, all light sources can be operated with a minimum operating current, which ensures the stability of the light. The color rendering of the non-fluorescent regions can be set by the individual settability of the light sources which can be set such that fluorescent and non-fluorescent regions appear to be approximately equally bright.

Abstract: Systems and methods for conducting designated reactions that include a fluidic network having a sample channel, a reaction chamber, and a reservoir. The sample channel is in flow communication with a sample port. The system also includes a rotary valve that has a flow channel and is configured to rotate between first and second valve positions. The flow channel fluidically couples the reaction chamber and the sample channel when the rotary valve is in the first valve position and fluidically couples the reservoir and the reaction chamber when the rotary valve is in the second valve position. A pump assembly induces a flow of a biological sample toward the reaction chamber when the rotary valve is in the first valve position and induces a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position.

Abstract: An optical detection device having a light detection device and a light emission device is arranged such that the light detection side of the light detection device is optically coupled to a light emission side of a light source array of the light emission device via an examination region. The light detection device generates an electrical signal n response to light that reaches the light detection side. The light source array includes a plurality of separately actuatable electric light sources which are arranged in a matrix structure or two dimensional geometric arrangement. The object to be examined can be arranged in a desired fashion, and the light emitted by the light sources radiates via the examination region on the light detection side of the light detection device. An optical reduction is system is arranged in the beam path from the light emission side to the examination region and is configured to demagnify the light pattern which is emitted by the light sources.

Abstract: A process characteristic parameter determination system uses a process model and a tuning module to accurately determine a value for a process characteristic parameter within a plant without measuring the process characteristic parameter directly, and may operate on-line or while the process is running to automatically determine a correct value of the process characteristic parameter at any time during on-going operation of the process. The process characteristic parameter value, which may be a heat transfer coefficient value for a heat exchanger, can then be used to enable the determination of a more accurate simulation result and/or to make other on-line process decisions, such as process control decisions, process operational mode decisions, process maintenance decisions such as implementing a soot blowing operation, etc.

Abstract: There is provided an inspection device allowing surely measuring irregular-shaped parts such as a bevel of a wafer while saving space. An inspection device 100 is provided with outer periphery illuminating units 11, 111 for illuminating an outer peripheral region AP of a wafer WA being a target, and an outer periphery imaging unit that images the outer peripheral region AP of the wafer WA. The outer periphery illuminating units 11, 111 have arcuate illuminating units 11a, 111a that are arranged along a partial region of a circumference CI centered on a reference axis SA and illuminate a predetermined region A1 on the reference axis SA, and the reference axis SA of the arcuate illuminating units 11a, 111a extends in a direction crossing the tangent direction along which an outer peripheral part UA of the wafer WA extends.

Abstract: In accordance with one or more implementations of the apparatus, system and methods described, a sample measurement device is provided that is configured to measure the color of a sample. The sample measurement device includes at least one light source configured to illuminate the sample; at least one light sensor configured to output a signal in response to light emitted by light source and reflected off the sample being received by at least a portion of the light sensor; and a processor configured to receive the signal and calculate a color value for the sample, the processor configured to calculate the color value by at least adjusting the signal using a calibration factor.

Abstract: Provided herein are devices, systems, and methods for detection of sensors, such as light-detectable sensors. The devices or systems as described herein may comprise an illumination assembly configured to direct a light to at least a well of a plurality of wells in the array of wells and a detection assembly configured to detect a signal from the well.

Abstract: The method of analyzing one or more samples arranged in sample receptacles of a platform that is configured to receive a plurality of separate samples includes the steps of measuring electromagnetic radiation transmitted or emitted by each sample, repeating the measurement a plurality of times at predetermined intervals, on the basis of each measurement, forming a result matrix comprising a plurality of cells, each cell of the result matrix corresponding to a sample receptacle of the plat-form, wherein a measurement value of each sample is used as an input for determining the visual properties of the respective cell in the result matrix, and displaying the results as consecutive matrixes in respect of time.

Abstract: An airborne biological particle monitoring device collects particles floating in air. The monitoring device includes a processor, a camera sensor, and a set of approximately monochromatic illumination sources that correspond to a set of spectral curves. The camera sensor captures images of the particles providing a spectral analysis of the particles. The processor analyzes the images to identify the collected particles.

Abstract: Systems and methods for three-dimensional fluorescence polarization excitation that generates maps of positions and orientation of fluorescent molecules in three or more dimensions are disclosed.

Abstract: Provided is an observation device including: a stereo image-acquisition optical system that acquires images of cells floating in a culture fluid inside a culture vessel; and an analyzer that calculates a cell density of the cells on the basis of the images acquired by the stereo image-acquisition optical system, wherein the analyzer identifies a three-dimensional position of each of the cells included in the images and calculates the cell density on the basis of the number of cells present within a predetermined three-dimensional region.

Abstract: An apparatus adapted to perform spectrometric measurements, said apparatus comprising a tunable laser light source adapted to generate a laser light with an excitation wavelength supplied to an optical sensor which produces a sample specific response light signal; an optical reference filter adapted to measure laser light with the excitation wavelength fed back as a reference signal to provide wavelength calibration of the tunable laser light source; at least one optical measurement filter adapted to measure the sample specific response light signal produced by the optical sensor, wherein the optical reference filter and the at least one optical measurement filter are thermally coupled to maintain a constant wavelength relationship between the filter characteristics of the optical filters.

Abstract: A liquid evaluation system can include a cartridge including a channel configured to pull a liquid into the channel by capillary action. The cartridge can include a first plate and a second plate located in close proximity to the first plate. An internal facing surface of each plate can include a corresponding region forming the channel. Each of the regions can have an affinity for the liquid. The close proximity of the plates and the regions having an affinity for the liquid cause the liquid to be pulled into the channel by capillary action. The cartridge can include one or more additional attributes and/or the system can include one or more additional components for performing the evaluation.

Abstract: A remote sampling sensor for determining characteristics of a sample includes measurement optics and an insertion probe. The measurement optics are configured to emit light and detect returned light. The insertion probe includes a chamber, the chamber being configured to permit the sample to enter the chamber, an insertion tip at a distal end of the insertion probe, and a retro-reflective optic adjacent the insertion tip. The retro-reflective optic is configured to return the light from the measurement optics through the chamber to the measurement optics. The insertion probe is configured to be remotely located from the measurement optics.

Abstract: Disclosed are methods for detecting a presence or absence of an antimicrobial surface coating including applying at least one detectable fluorophoric dye compound to a substrate, irradiating the surface of the substrate with ultraviolet radiation in the 100-415 nm wavelength range to excite the detectable fluorophoric dye compound, observing fluorescence of the excited fluorophoric dye compound, and determining the presence or absence of the antimicrobial surface coating based on the observed fluorescence. Further disclosed are antimicrobial surface coating solutions, methods for their application, and methods for confirming the presence and coverage of antimicrobial surface coatings.

Abstract: A label-free optical device for near real time quantification of the multifractal micro-optical properties of a sample includes a source of broadband light; a tunable filter that receives at least a portion of the broadband light and then transmits narrowband light, whereby a specific band of light is selected to avoid unwanted absorption of light by the sample; where the narrowband light is configured to illuminate a selected area of the sample, and in response elastically-scattered light is dispersed from the sample; a light collection device configured to collect at least some of the elastically-scattered light; where at least some of the collected elastically-scattered light is configured to be transmitted to a detector by the light collection device, and the detector is configured to record a light scattering signal; and where the detector is configured to perform light scattering signal measurements at multiple angles or wavelengths to determine a refractive index multifractality of the sample.

Abstract: The invention relates to a method and a device for optically exciting a plurality of analytes (12) in an array of reaction vessels (14) and for sensing fluorescent light from the analytes (12), wherein excitation light from an excitation light source (38) is supplied to the analytes (12) and wherein fluorescent light from the analytes (12) is simultaneously supplied to a fluorescent detector (34), wherein a plurality of beams (30) of the excitation light is directed into the reaction vessels (14) below the cover (28) through an array of holes (26) in a cover (28) arranged above the array of reaction vessels (14) and a plurality of beams (32) of the fluorescent light from the reaction vessels (14) reaches the fluorescent detector (34).

Abstract: A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.