Abstract: The present invention provides a biochip reader for reading with a detector the fluorescence image information from genes to which fluorescent substances are stuck and which are poured into biochip cells, by emitting coherent light beams such as laser light onto the cells as excitation light. The present invention aims to achieve a biochip reader that is small, cheap, and highly durable.

Abstract: The present invention provides a biochip reader for reading with a detector the fluorescence image information from genes to which fluorescent substances are stuck and which are poured into biochip cells, by emitting coherent light beams such as laser light onto the cells as excitation light. The present invention aims to achieve a biochip reader that is small, cheap, and highly durable.

Abstract: Systems and methods for automated laser capture microdissection are disclosed. High throughput microdissection is provided by using cell procurement and multi-imaging tools for pre-selecting cells of interest. Novel methods of computer-controlled cap transfer along with automated multi-slide and multi-cap placements, and automated slide and cap detection are provided. The systems and methods provide the advantages of increased speed and much lower rates of contamination.

Abstract: The present invention provides a biochip reader for reading with a detector the fluorescence image information from genes to which fluorescent substances are stuck and which are poured into biochip cells, by emitting coherent light beams such as laser light onto the cells as excitation light. The present invention aims to achieve a biochip reader that is small, cheap, and highly durable.

Abstract: A method of detecting wear on a substrate including coating a composition that includes a fluorescent compound on the surface of a first substrate, exposing the coated surface to wear, exposing the coated surface to radiation capable of exciting the fluorescent compound, and detecting the presence or absence of fluorescence.

Abstract: The present invention provides a biochip reader for reading with a detector the fluorescence image information from genes to which fluorescent substances are stuck and which are poured into biochip cells, by emitting coherent light beams such as laser light onto the cells as excitation light. The present invention aims to achieve a biochip reader that is small, cheap, and highly durable.

Abstract: A forensic light source uses a pair of filter wheels to achieve a combination of the characteristics of filters on different wheels and may include a housing which supports the light, filter wheels and other parts with shock absorbing members such as rubber washers or the like to achieve a shock resistant housing. A light source and a plurality of light filters are contained within the housing. The light source is powered by an external power supply, thus making the light source light weight.

Abstract: A fluorescence detecting device is configured so that a semiconductor integrated circuit substrate includes a photodiode and a signal detecting circuit for detecting charges obtained as a result of photoelectric conversion by the photodiode, and a fluorescence reaction vessel where a fluorescence reaction occurs is arranged above the foregoing photodiode. Furthermore, in the device, an excitation-light-entry preventing layer is provided at one or more of a surface portion of the photodiode and a position between the photodiode and the fluorescence reaction vessel.

Abstract: Some of the key optical components of lithography lasers are very sensitive to intensive UV radiation. Intensive UV radiation can cause color center formation in these components. The color centers are reason for laser energy dropping, worse laser-bandwidth and limited life-time. The on-line monitoring of the color-center formation during operation of the lithography lasers detecting laser induced fluorescence and investigation of the fluorescence spectrum can be helpful for maintenance of lithography lasers. The fluorescence signal is analyzed and delivers information about optics quality.

Abstract: At the time of analytical measurement of a sample by the fluorescence measuring device or the phosphorescence measuring device, both the optical path of exciting light emitted from the light source to the sample and the optical path of fluorescence or phosphorescence emitted from the sample to the detection unit are shut off. Both are shut off by one chopper.

Abstract: A fluorescent optical imaging system (10) produces two separate spots (S1 and S2) on a sample (12) by a pair of excitation laser beams (B1 and B2) that are generated by first and second lasers (L1 and L2). Excitation laser beams (B1 and B2) pass at slightly different angles, first through an aperture (15) of a 45° fold mirror (13), and then through an objective element (14). As a result, emission light beams (16, 18) are generated from each illuminated spot (S1 and S2) and are reflected and redirected by mirror (13) through a secondary lens (19) before reaching one of two detectors (PMT 1 and PMT 2). Emission beam (16) reflects off a second 45° mirror (22) prior to reaching detector (PMT 1), while emission beam (18) travels directly to (PMT 2). If desired, optical separation elements (24), such as dichroic filters, prisms, or gratings, can be positioned in front of each detector (PMT 1 and PMT 2).

Abstract: A multicolor particle analyzer and method is described. The particles each of which either naturally fluoresce or are tagged to fluoresce at distinctive wavelengths are caused to flow through an analyzing volume where fluorescence is excited by an impinging light beam. A tunable optical filter repetitively and sequentially passes emitted light at each of the characteristic wavelengths as each particle travels through the analyzing volume and the light transmitted through the optical filter is received by single detector which provides output signals representative of each distinct wavelength of light emitted by the particle.

Abstract: A method and apparatus for detection of a particular material, such as photo-resist material, on a sample surface. A narrow beam of light is projected onto the sample surface and the fluoresced and/or reflected light intensity at a particular wavelength band is measured by a light detector. The light intensity is converted to a numerical value and transmitted electronically to a logic circuit which determines the proper disposition of the sample. The logic circuit controls a sample-handling robotic device which sequentially transfers samples to and from a stage for testing and subsequent disposition. The method is particularly useful for detecting photo-resist material on the surface of a semiconductor wafer.

Abstract: A flame atomization device, especially for an atom absorption spectrometer, is disclosed. The flame atomization device includes at least one mixer chamber and an atomizer leading into the mixer chamber, and a burner head mounted on the mixer chamber's burner connection piece. A slip casing is mounted so that it can slide between a closed position and an open position in relation to the burner connection piece while, in the open position, at least one pressure reduction outlet is opened in the burner connection piece.

Abstract: An optical apparatus including an illuminating optical system for illuminating a sample with light from a light source, a first wavelength selecting member, a light beam splitter for directing illuminating light toward the sample, an objective lens, a detector, and a second wavelength selecting member disposed between the objective lens and the detector, in which the first wavelength selecting member has a wavelength selecting element for selectively transmitting light having wavelengths in a predetermined region out of the illuminating light, the second wavelength selecting member has a wavelength selecting element for transmitting light having a predetermined wavelength out of light reflected by the sample, and the light beam splitter has an optical element having transmittance not lower than 85% and reflectance not higher than 14% in a wavelength region not shorter than 400 nm and not longer than 700 nm. The optical apparatus permits observing fluorescent light at the same time with a high efficiency.

Abstract: The optical properties of water impounded in reservoirs such as large tanks with closed covers and in uncovered reservoirs are monitored to detect harmful substances. The system and method comprises illuminating the water with an artificial light source within the enclosure for monitoring of the reservoir. Radiance and irradiance fluxes are measured by one or more multiband radiometers. Changes in radiance and irradiance are used to calculate spectral attenuation coefficients of the water. Fluorescence spectra are measured. A computer look-up table of known absorption and emission spectra is compared to the spectral attenuation to determine if a substance has been detected.

Abstract: The present invention relates generally to the field of biochemical laboratory. More particularly the invention relates to the improved and more efficient instrumental features of equipment used as e.g. fluorometers, photometers and luminometers. The object of the invention is achieved by providing an optical measurement instrument where there is an interface (218, 223, 233a, 233b, 238) for a changeable optical module (240), the interface being adapted for at least one excitation beam and at least two emission beams. This allows performing various types of measurements by changing an optical module. The change of module and related parameters can be performed automatically controlled by software. It is also possible to easily upgrade the instrument for new types of measurements by just providing the instrument with a new optical module and the related software.

Abstract: A characteristic response of a medium to an excitation transient of predetermined duration which causes the medium to emit a series of signals over a period of time which is long relative to the duration of the excitation transient is assessed. The signals are detected and the duration of each interval between successive signals is measured. A relationship relating the interval between the excitation transient and the emission of each signal to the interval between each signal and the preceding signal in the series is derived to represent the characteristic response.

Abstract: An apparatus and method for examining particles in a flow stream of a flow cytometer, employing incoherent light sources, such as light emitting diodes (LEDs), and detectors. The light emitting diodes operate as the excitation light sources and emit light toward said flow stream, and the detectors detect light, in particular, fluorescent light, emanating from the particles in response to the excitation light striking the particles. A controller controls each of the light emitting diodes to emit their excitation light for a predetermined period during which the excitation light radiates onto particles of interest. The controller evaluates the detected light to ascertain characteristics of the particles, such as particle size, density and granularity. The apparatus and method can further employ one or more coherent and homogenous light emitting devices, such as a laser, as an additional excitation light source.

Abstract: In one aspect, the present invention provides kinetic spectrophotometers that each comprise: (a) a light source; and (b) a compound parabolic concentrator disposed to receive light from the light source and configured to (1) intensify and diffuse the light received from the light source, and (2) direct the intensified and diffused light onto a sample. In other aspects, the present invention provides methods for measuring a photosynthetic parameter, the methods comprising the steps of: (a) illuminating a plant leaf until steady-state photosynthesis is achieved; (b) subjecting the illuminated plant leaf to a period of darkness; (c) using a kinetic spectrophotometer of the invention to collect spectral data from the plant leaf treated in accordance with steps (a) and (b); and (d) determining a value for a photosynthetic parameter from the spectral data.

Abstract: A spectroscopic probe includes a monolithic block (10) of transparent material and GRIN lenses (20, 22, 24). Light for illuminating a sample is delivered by an optical fiber (31), and light scattered by the sample is output by an optical fiber (33). The block (10) has opposing angled faces (12A, 12B), coated with a dichroic filter coating and a reflective coating respectively. A method for making blocks (10) is also disclosed, in which the coatings are first provided on a large sheet, and blocks (10) are then cut from the sheet using angled cuts. Since the coated block reduces the number of components required, the probe can be miniaturized, e.g. for use in an endoscope.

Abstract: The light detection system includes at least two light detection elements. Each element is responsive to a selected light color in a first mode, and each element has a different sensitivity and is responsive to a single color in a second mode for enhanced dynamic range. A preferred embodiment includes four light detectors for responding to four separate colors or for responding with enhanced dynamic range to two colors. Embodiments are disclosed using a mirror/beam splitter arrangement to switch between modes and an embodiment including dichroic filters serving as fractional beam splitters.

Abstract: A lightweight hand-held skin abnormality detection system includes a source of excitation light that causes tissue under examination to produce fluorescence light. The fluorescence light produced along with the beam of reference light is provided to a beam splitter which divides the fluorescence light and the reference light into separate optical channels. Each optical channel produces an image of the tissue under examination. A passive optical combiner superimposes the image produced by each optical channel for viewing by a user.

Abstract: A non-invasive portable apparatus for analyzing the performance of gas-filled window glazing units is disclosed. The operation of the apparatus is based on discharging the spacing between the panels (2a, 2b) of the window glazing unit (1) by applying rapidly alternating electrical field to the spacing between the panels of the window glazing unit, on collecting and analyzing the emitted discharge light in different structural intervals. The discharge is created by a needle-like electrode (5), and the inner conducting layer (2a) of the glazing unit serves as another electrode. The localization of the discharge in the vicinity of the end of the needle-like electrode (5) makes it possible to collect the emitted light without routine adjustment of the optical system.

Abstract: A universal microplate analyzer capable of carrying out measurements on samples contained in the wells of microplates by fluorescence, absorbance, luminescence employs at least two light sources and optical fiber channels for directing excitation light to the sample wells. Flexibility of operation is provided by arrays of mirrors, apertures, and polarizers which can be positioned as required for the analysis to be carried out.

Abstract: Apparatus for detecting light emitted by an assay sample is provided, in which light emitted by the sample is collected for transmission to a photosensitive detector such as a charge coupled device (CCD) (74) by an optical fiber 78 bundle. The cross-sectional area of the optical fiber bundle corresponds to the area of the sample, the end of which is located close to the sample for detecting any light emitted therefrom. Selected fibers (30) of those making up the bundle may be separated from the remainder and extend to a source of excitation radiation (76) to convey excitation radiation to the sample. The remaining fibers (32, 38) serve to collect emitted light and provide a light path to the photosensitive detector (74). A blocking filter and an interference filter are placed between the fiber bundle and the detector. The blocking filter may be between the bundle and the interference filter or between the latter and the detector.

Abstract: The invention provides systems and methods for analyzing mixtures and, particularly during processing of the mixtures. The invention provides systems and methods for analyzing mixtures and, particularly, for analyzing mixtures during processing. The methods and systems utilize fluorescence to non-invasively analyze one or more components of the mixture. The analysis may provide a variety of compositional information such as the chemical identity of components, the concentration of components, the uniformity of the mixture and other information. A number of different types of processing equipment can be configured to provide the analysis and, accordingly, the analysis may be performed during a number of processing operations. The system and methods are particularly useful in processing mixtures which are preferably homogeneous blends, such as pharmaceutical products.

Abstract: A method is provided for sorting white paper from a stream of paper wherein the concentration of white paper is less than the concentration of non-white paper. The method includes identifying the white paper by detecting the presence of an optical characteristic of the white paper as the stream of paper is passed by a sensor. Then the white paper is ejected from the stream of paper by controlling one or more actuators directed against the white paper, and thereby separating the white paper from the non-white paper. The method is particularly applicable to the sorting of white paper during curbside recycling.

Abstract: A system and method for tuning a pulsed-flame photometric detector including providing a carrier gas within a predetermined gas flow range to the pulsed-flame photometric detector, providing at least one combustible fuel to the pulsed-flame photometric detector, applying voltage to an igniter coil associated with the pulsed-flame photometric detector, verifying that the pulsed-flame photometric detector is pulsing above a lower predetermined frequency and above a higher predetermined frequency, adjusting the gas flow of the at least one combustible fuel to the pulsed-flame photometric detector so that at least one cycle of ignition, propagation and then termination is present, heating a container to a predetermined temperature, allowing the at least one carrier gas to flow within the container, housing a tuning compound, and monitoring a width of a sulfur emission resulting from the combustion of the tuning compound to evaluate the width of sulfur emission signal all through a controller.

Abstract: The invention relates to a device for photometric measurement of several samples (12) which are exposed to radiation from a light source (14) associated therewith. The light modified by the samples (12) is intercepted by an optical device (19) and is guided as a sum of all the light radiated from all samples (12) to at least one sensor (27) for measuring the intensity and evaluation thereof in an evaluation device (32) arranged downstream. The light sources (14) are controlled individually by a control device (31) and the evaluation device (32) and the control device (31) are controlled in such a way that the evaluation device (32) separates the light from each sample (12) from the light of the other samples. The invention is characterized in that the control device (31) modulates the light source (14) with various signals which are pre-assigned to the evaluation device (32) and used to determine the light signals of the individual samples (12) from the sum signal received by the sensor (27).

Abstract: Methods and apparatus for generating multispectral images of tissue. The multispectral images may be used as a diagnostic tool for conditions such as cervical cancer detection and diagnosis. Primary radiation is produced with an illumination source. The primary radiation is filtered to select a first wavelength and a first polarization. Tissue is illuminated with the filtered primary radiation to generate secondary radiation, which is filtered to select a second wavelength and a second polarization. The filtered secondary radiation is collected with a detector, and a plurality of multispectral images of the tissue is generated according to different combinations of first and second wavelengths and first and second polarization with an analysis unit in operable relation with the detector. Apparatus utilizing the invention include endoscopes and colposcopes.

Abstract: An image information reading apparatus of the optical head scanning type is designed to maintain the light amount to be detected at an uniform level while an optical head shifts its scanning position. A sample 10 is irradiated with a laser beam L by an optical head 50 and fluorescence K emitted by the sample 10 is led to a PMT 40, wherein the fluorescence K proceeding toward the PMT 40 as a diverging beam is refracted by a collective lens 90 located in the optical path between the optical head 50 and the PMT 40 so that the beam of the fluorescence K enters the PMT 40 with a contracted beam diameter.

Abstract: A DNA sample added with a plurality of fluorescent marks is irradiated with excitation lights having a plurality of wavelengths and when fluorescence intensities are detected separately, the excitation light and a position on the sample are relatively changed over a desired area once to thereby detect the plurality of fluorescent marks at high speed.

Abstract: The invention relates to a device and a method for optically measuring the concentrations of a substance contained in a fluid medium. With the solution according to the invention the accuracy of measurement is to be increased in accordance with the object over a greater period without any additional calibration measurements, and ageing of a fluorescent substance within a layer is to be taken into consideration, in particular, and the long-time drift of such a measurement system is to be reduced. To achieve this, a layer containing such a known fluorescent substance or layer system are employed in which fluorescence is excited. The fluorescence changes depending on a substance concentration or a pH-value, and the intensity varying correspondingly in time or phase shift of the fluorescent light is allowed to be measured with at least one optical detector with the known intensity or phase of the fluorescence exciting light.

Abstract: Apparatus and methods are disclosed for imaging surfaces that comprise a sample. One embodiment of the present invention is an imaging apparatus comprising a holder for a surface, a light source adapted to illuminate the surface, a diffractive element between the holder and the light source and an imaging detector adapted to receive light from the surface. In a method for imaging a surface comprising a plurality of discrete features, light is selectively diffracted on to one or more predetermined features of the surface at a predetermined point in time. This step is repeated until substantially all of the features on the surface are illuminated. Light is detected from the surface to thereby image the surface.

Abstract: The present invention relates to the measurement of the time dependence of light emitted by a material after it is excited by a light or electric pulse. An embodiment of this invention utilizes a CCD camera and a mask with slits for time resolved spectroscopy of fluorescent materials illuminated with a light pulse. By converting a slit in space to a gate in time, the whole decay curve of the fluorescence may be obtained with a single material-exciting light pulse. The systems and methods of the present invention are particularly suitable for DNA and protein study.

Abstract: Fluorescently marked targets bind to a substrate 230 synthesized with polymer sequences at known locations. The targets are detected by exposing selected regions of the substrate 230 to light from a light source 100 and detecting the photons from the light fluoresced therefrom, and repeating the steps of exposure and detection until the substrate 230 is completely examined. The resulting data can be used to determine binding affinity of the targets to specific polymer sequences.

Abstract: A method for sorting products including the steps of conveying the products in a product stream, scanning the products in the product stream casting light onto the products on the basis that certain products will emit light, observing emitted light from the products, making a selection of certain products from the product stream as a function of the observed light, and separating certain products from the product stream as a function of the selection. A device is provided that is arranged to sort the products according to the method of the invention and includes a light device that is arranged to cast light onto products and an observation device that is arranged to observe light emitted from the products. A selection device is also provided that is arranged to make a selection of whether to separate certain products from the product stream observed by the observation device.

Abstract: Disclosed is a portable fluorescence correlation spectroscopy instrument that includes an excitation source, at least one of a light focusing element positioned to receive light emitted by the excitation source, a detector for detecting light, the detector positioned to receive light emitted by a sample excited by the excitation source, and a correlator coupled to the detector, the correlator for processing data received at the detector and providing data including autocorrelation data, crosscorrelation data, or a combination thereof.

Abstract: An instrument for measuring reliably and instantaneously the chemical quality of cooking oil, and for distinguishing between color changes due to chemical changes and color changes due to the presence of minute size food particles in various oils.

Abstract: A method and apparatus for detecting a fluorescent substance tagged to a microparticle are described. The device comprises a single capillary flow carrier system for transporting the microparticle past a selected location, a source of electromagnetic radiation for irradiating the substance tagged to the microparticle, and a detection system for measuring fluorescent light emitted from the substance at the selected location. The method comprises transporting the microparticle to a selected location, irradiating a fluorescent substance tagged to the microparticle, and measuring the fluorescent light emitted from the fluorescent substance at the selected location.

Abstract: A downhole optical apparatus includes an LED source, reflectance and fluorescence detectors, a plurality of fibers, a dichroic mirror (DM), a beam splitter/coupler, a probe, a short-pass filter (SP), a dichroic long-pass filter (LP), and a lens. Source light filtered by the SP is fed to the DM which deflects light of desired wavelengths only. The deflected light is focused by the lens onto a fiber and is ultimately injected into an oil flow by the probe. Light reflected by oil or fluorescing therefrom is received by the probe, and split by the splitter. A small portion is received by the reflectance detector. A large portion is received by the lens and directed to the DM which deflects reflected light and passes light at longer fluorescing wavelengths. Passed light is further filtered by the DM and LP to eliminate remnants of the reflected light, and provided to the fluorescence detector.

Abstract: A fluorescent optical imaging system (10) produces two separate spots (S1 and S2) on a sample (12) by a pair of excitation laser beams (B1 and B2) that are generated by first and second lasers (L1 and L2). Excitation laser beams (B1 and B2) pass at slightly different angles, first through an aperture (15) of a 45° fold mirror (13), and then through an objective element (14). As a result, emission light beams (16, 18) are generated from each illuminated spot (S1 and S2) and are reflected and redirected by mirror (13) through a secondary lens (19) before reaching one of two detectors (PMT 1 and PMT 2). Emission beam (16) reflects off a second 45° mirror (22) prior to reaching detector (PMT 1), while emission beam (18) travels directly to (PMT 2). If desired, optical separation elements (24), such as dichroic filters, prisms, or gratings, can be positioned in front of each detector (PMT 1 and PMT 2).

Abstract: A portable polymerase chain reaction DNA amplification and detection system includes one or more chamber modules. Each module supports a duplex assay of a biological sample. Each module has two parallel interrogation ports with a linear optical system. The system is capable of being handheld.

Abstract: Apparatus and procedure for the in situ measurement of quantities of polymer printed onto supports

Abstract: The invention relates to a fluorescence-measuring system as can be employed for high-throughput screening in drug development. The arrangement for fluorescence excitation contains a two-dimensionally extended sample-receiving device and at least two illumination sources for exciting the fluorescence of the samples. The illumination sources are extended linearly and arranged in such a way that the illuminated area of the sample-receiving device is homogeneously illuminated directly or via deflecting mirrors at an opening angle of ≦30°.

Abstract: A photomultiplier which detects feeble light emitted by the irradiation of excitation light on an image carrier carrying an image related to a living organism has an integration circuit is DC-coupled with a photomultiplier to convert a current signal output from the photomultiplier, to a voltage signal. The voltage signal converted by the integration circuit is logarithmically compressed with a logarithmic conversion circuit. An offset compensation circuit is provided for reducing an offset voltage that could occur because of charge injection in the integral action of the integration circuit.

Abstract: Apparatus and methods are provided for sensing the presence of bright white paper on a conveyor of a paper sorting system. The conveyor is constantly illuminated with ultraviolet light. When bright white paper is present in the inspection zone of the conveyor, it will re-radiate fluorescent light energy as a result of the ultraviolet light. Periodically, the inspection zone of the conveyor is illuminated with a second light source in the visible light spectrum. Light is collected from the inspection zone of the conveyor, including reflected light from the secondary source and including emitted fluorescent light energy as a result of the ultraviolet light falling on bright white paper. Periodically a microprocessor associated with the sensor senses reflected light from the second source to determine whether any object if present on the conveyor. The microprocessor then senses the level of fluorescent light energy being emitted from any object on the conveyor.

Abstract: Systems and methods for automated laser capture microdissection are disclosed. High throughput microdissection is provided by using cell procurement and multi-imaging tools for pre-selecting cells of interest. Novel methods of computer-controlled cap transfer along with automated multi-slide and multi-cap placements, automated slide and cap detection are provided. The systems and methods provide the advantages of increased speed and much lower rates of contamination.

Abstract: The invention relates to a device for carrying out biochemical fluorescence tests by means of which the different biochemical interactions can be detected. With the aid of the invention it shall be allowed for a very large number of individual samples to be detected low costly and with a high sensitivity, and in addition it shall be achieved a high spatial resolution. According to the invention this object is solved with a device by means of which linearly polarized light of a laser diode is directed upon a plate-shaped carrier through an optical arrangement comprising at least one polarization beam splitter, a quarter-wave plate and a focussing optical element. In addition to binary, optically detectable information structures a plurality of fluorophore-marked samples is discretely arranged as well on the carrier rotating about an axis.