Abstract: A laser whose emission is modulated by ultrasound is presented. The laser is usually micron-sized. In response to ultrasound modulation, the laser emission increases and decreases. Such a change in emission can be detected by external optical detectors. This type of laser can be used as a new type of imaging modality, in which laser emission in combination with sound waves or ultrasound waves, is used for imaging Laser emission has a much narrower spectral linewidth and stronger intensity than fluorescence and therefore is able to achieve higher sensitivity, whereas sound waves are used to provide a better spatial resolution of the laser emission from the laser. In ultrasound modulated laser based imaging, multiple lasers can be placed inside cells or tissues.

Abstract: Methods and systems are described that enable three-dimensional (3D) imaging of objects. One example system includes a light source that produces multiple light beams having specific spectral content and polarization states. The system also includes phase masks that modify the intensity or phase of the light, and projection optics that allow simultaneously projection of at least three fringe patterns onto an object having particular phase, polarization and spectral characteristics. The detection system includes a camera that simultaneously receives light associated with the at the fringe patterns upon reflection from the object, and a processing unit coupled to the camera unit that determines one or both of a phase or a depth information associated with the object. The system and associated methods can efficiency produce 2D images of the object and allow determination of characteristics such as surface profile. The disclosed systems and methods can be effectively implemented with moving objects.

Abstract: Provided is a determination method capable of non-destructively and simply determining a state of a sphere that is an aggregate of a plurality of cells. A phase difference image of a sphere that is an aggregate of a plurality of cells is generated from a hologram obtained by imaging the sphere, and a state of the sphere is determined on the basis of the phase difference image and a shape index value corresponding to a shape of the sphere.

Abstract: A determination method of non-destructively and easily determining a state of an aggregate of a plurality of cells formed by three-dimensional culture is provided. A determination method according to the disclosed technology includes generating a phase difference image of an aggregate of a plurality of cells from a hologram obtained by imaging the aggregate, deriving a first index value that indicates a randomness of an array of a phase difference amount in a plurality of pixels constituting the phase difference image, and determining a state of the cells constituting the aggregate on the basis of the first index value.

Abstract: A metrology frame configured to receive and secure a workpiece in preparation for an interferometric determination of a spatial profile of the workpiece with the use of one or more retroreflectors removably cooperated with the frame in known pre-determined spatial relationship with respect the fiducial features of not only the workpiece but those of the metrology frame itself. The metrology frame is necessarily devoid of a holographic optical element, while the measurement apparatus containing such metrology frame employs a hologram configured to generate at least one alignment optical wavefront that spatially converges on the retroreflector.

Abstract: A Pulsed Thermography (PT) system and method is provided utilizing a long duration pulse in combination with a radiant heat shield as a non-destructive testing method for quantitatively measuring defect depths within a 3D printed part and for characterizing layer-by-layer surface defects in the 3D printed part.

Abstract: Apparatus and method for detecting wavefront aberration of an objective lens, comprising a wavefront detection system, a planar mirror, and a planar mirror adjusting mechanism; the objective lens is placed between planar mirror and wavefront detection system; planar mirror is positioned at focal point of the objective lens. A test wavefront emitted by wavefront detection system passes through the objective lens, gets reflected by the planar mirror, and t passes through the objective lens again; the wavefront detection system receives and detects the test wavefront to derive a phase distribution thereof; an angle of the planar mirror tilts at is adjusted to obtain different return wavefronts; a polynomial for expressing wavefront aberration is selected, and expressions corresponding to all the return wavefronts are calculated; result of fitting the wavefront aberration of the objective lens when expressed by the selected polynomial is derived through fitting with the polynomial.

Abstract: The present disclosure relates to methods and systems for measuring deflection of blades of a wind turbine. Examples include a light emitting and collection device mounted to the nacelle and configured to emit light in a direction within a substantially vertical plane. Examples include a method for operating a wind turbine including emitting light above a hub, receiving the light when reflected by a blade of the wind turbine, and, if the level of blade deflection is above a threshold, reducing blade loading of the blade before the blade reaches a vertically downward position. Examples include a method for monitoring deflection of a rotor blade of a wind turbine comprising emitting a light sheet, collecting reflections of the emitted light, and determining deflection of the rotor blade by determining a time during which the blade reflects the emitted light sheet.

Abstract: The invention discloses a digital laser holography-based rapid lens center offset detection device, which relates to the technical field of lens detection and includes a spherical wave emission device, a reticle, a lens to be detected, an image sensor and a computer. The device is simple and stable in structure, and a complex optical receiving system and mechanical scanning are avoided. A detection method is high in efficiency and measurement accuracy, a process is simple, and a lens with an infinitely great focal length may be detected.

Abstract: A method for checking a tyre in a tyre production line. A portion of outer surface of the tyre is deformed to form an elastically deformed portion of inner surface and an elastically deformed portion of outer surface while illuminating the elastically deformed portion of outer surface with a first diffused light radiation emitted by a first light source and illuminating the elastically deformed portion of inner surface with a second grazing light radiation emitted by a second light source. Images of the deformed and illuminated portions of outer and inner surfaces are acquired through cameras, and the tyre is set in relative rotation around its rotation axis with respect to the first and second light sources during deformation and illumination.

Abstract: An apparatus for checking a tyre, having a support, a deformation system, a lighting arrangement, a moving member and a processing unit. The deformation system is configured to form an elastically deformed portion of inner surface and an elastically deformed portion of outer surface on the tyre. The lighting arrangement includes light sources and cameras to illuminate and detect deformed inner and outer surface portions of the tyre, and generate corresponding control signals. The moving member sets the support in relative rotation with respect to the light sources and the deformation system around a rotation axis of the tyre. The processing unit is configured so that while the light sources illuminate the deformed portions of outer and inner surfaces, the deformation system acts and the moving member imparts the relative rotation.

Abstract: An image creation unit of a server creates and stores a plurality of phase images or the like having different resolutions on the basis of holographic data collected by a measuring terminal. In response to an image transmission request according to an operation, an image transmission processing unit of the server extracts data of an image corresponding to an observation range after the movement from an image of an appropriate resolution and transmits the data to the browsing terminal. In the browsing terminal, a display image is formed by overlaying a high-resolution phase image corresponding to only an observation range to be displayed on low-resolution phase image of an observation target area.

Abstract: An interferometric optical device that measures the curved wall shape of a cylindrical object and includes: an interferometric optical system that emits measurement light at the curved wall of the object, collects the light reflected by the object, and creates a composite wave that combines the reflected light and a reference light; a rotation drive assembly that is connected to the interferometric optical system and rotationally displaces the interferometric optical system centered about a rotation axis that coincides with a center axis of the cylindrical shape of the object; a sensor that acquires a two-dimensional distribution of the intensity of the composite wave using a plurality of photoreceptor elements arrayed two-dimensionally; and a computation device that computes the internal wall shape of the object based on the plurality of two-dimensional distributions acquired in a state where a rotation angle for the rotation drive mechanism varies.

Abstract: Microscope (2) comprising a coherent light source (4) producing a coherent light beam (7), a light beam guide system (6) comprising a beam splitter (14) configured to split the coherent light beam (7) into a reference beam (7a) and a sample illumination beam (7b), a sample holder (18) configured to hold a sample (1) to be observed, a sample illumination device (28) configured to direct the sample illumination beam (7b) through the sample and into a microscope objective (37), a beam reuniter (16) configured to reunite the reference beam and sample illumination beam after passage of the sample illumination beam through the sample to be observed, and a light sensing system (8) configured to capture at least phase and intensity values of the coherent light beam downstream of the beam reuniter.

Abstract: A digital holographic image-taking apparatus includes an illumination portion having a light emission surface for emitting illumination light toward an object, the illumination light having a specific wavelength in a coherent plane waveform; and an image sensor having an pixel array including two-dimensionally arranged pixels, the image sensor capturing an interference pattern generated based on the illumination light having acted on the object, in which the following conditional expression is satisfied: 0.0000001<Z2/S<16, where S represents the area of the light emission surface, and Z represents the distance from the light emission surface to the pixel array.

Abstract: An interference fringe pattern (ILR) between an inline spherical wave light (L) and an off-axis reference light (R) is recorded with a photo detector (4), and on which spatial-frequency filtering is applied to obtain a complex amplitude in-line hologram (JLR). A complex amplitude off-axis hologram (JOR) is derived by performing a spatial frequency filtering on a hologram (IOR) in which an object light (O) emitted from a microscopic subject illuminated with a spherical wave light (L) is recorded with a reference light (R), and the derived data is divided with data of the hologram (JLR) so that a complex amplitude in-line hologram (JOL) from which a component of the reference light (R) is eliminated is generated and recorded.

Abstract: The invention is an optical fingerprint sensor with prism module, including a shell; an optical prism arranged in the shell, the optical prism comprising a collection surface, a basal plane, a mirror surface and an output surface, there being a first included angle provided between the mirror surface and the collection surface, a second included angle provided between the mirror surface and the basal plane, a third included angle provided between the output surface and the collection surface, and a fourth included angle provided between the output surface and the basal plane; and an image sensing unit combined with the shell, comprising multiple luminous elements arranged beneath the basal plane of the optical prism correspondingly, and an image receiver and a lens arranged corresponding to the output surface.

Abstract: An apparatus for acquiring images of an interior surface of a tire includes at least one image acquisition module. Each image acquisition module is provided with a lighting source, an image acquisition device, and a reflector. The lighting source is arranged to project a beam of light onto a predetermined zone of the interior surface of the tire. The image acquisition device is arranged to acquire a reflected beam of light, which corresponds to light from the beam of light from the lighting source that is reflected off the predetermined zone of the tire. The reflector is positioned optically between the lighting source and the predetermined zone of the tire. Each image acquisition module may be provided with a plurality of lighting sources, image acquisition devices, and reflectors.

Abstract: A method of and system for generating secure tokens and transmission based on (TRNG) generated tokens split into shares includes steps of: generating keys by a hardware based True Random number Generator (TRNG); sieving/filtering the generated keys using statistical tests suite; selecting the random numbers of required width as tokens; applying number to image transformation for the tokens; applying steganography methods of injecting into an image carrier to the selected tokens; and splitting the steganographic contents into at least two shares using Share Generation Appliance (SGA), which is based on visual cryptographic methods. The generated secure tokens are well suited for seamless secured shopping including product verification.

Abstract: A tire inspection apparatus for the optical inspection of a tire (4) comprises a housing with a support surface (3) for the tire (4) to be inspected; several measuring heads (7, 8, 9, 10; 12, 13, 14, 15, 16, 17), each of which comprises an optical measuring system, for inspecting the inside tread surface (11) and/or the external lateral surface (18) of the tire (4); and a positioning device (21, 22, 23, 24) for moving some or all of the measuring heads (7-10; 12-17) into a rest position and into a measuring position. To improve a tire inspection apparatus of this type, the measuring heads (7-10; 12-17) and the support surface (3) are installed without freedom of rotation.

Abstract: A non-contact optical probe utilizes an optical reference surface that projects a curved test wavefront toward the test surface and detects it by creating curved interferometric fringes localized in space in front of the reference surface. When a point to be measured on the test surface intersects the location of the fringes, the condition is detected by the probe. Because the fringes are localized at a known position in space with respect to a reference system, the precise coordinate of the surface point can be established. Such localized fringes are preferably produced by a spectrally controllable light source. The curvature of the fringes ensures a sufficiently large angle of acceptance for the probe to capture light reflected from points of high surface slope. The probe is particularly suitable for coordinate measurement machines.

Abstract: A projection-type display device is connectively coupled to a mobile device (such as a smartphone) where the light generated by a small projection device is directed at a relatively transparent holographic optical element (HOE) to provide a display to an operator of the mobile device or a viewer. The projector and HOE may be configured to produce and magnify a virtual image that is perceived as being displayed at a large distance from the operator who views the image through the HOE. The HOE may comprise a volume grating effective at only the narrow wavelengths of the projection device to maximize transparency while also maximizing the light reflected from the display projector to the eyes of the operator.

Abstract: A method for operating a wind turbine (10). The wind turbine (10) is operated with variable rotational speed between predeterminable minimum and maximum rotational speeds. A characteristic variable (51) of an oscillation of the wind turbine (10) is detected. The wind turbine (10) includes a tower (14) and a rotor (13). An open-loop or closed-loop control device (36, 50) provides open-loop control or closed-loop control of the rotational speed of the rotor (13) between a minimum rotational speed and a maximum rotational speed during a power-supplying operation of the wind turbine. A sensor (40) detects a characteristic variable (51) of an oscillation of the wind turbine (10) and the minimum rotational speed is changed depending on the characteristic value (51) of the oscillation. The minimum rotational speed is altered depending on the characteristic variable (51) of the oscillation by the open-loop or closed-loop control device (36, 50).

Abstract: Methods and devices for reducing the dimensions of an incident light beam of large dimensions are disclosed. The method includes the dispatching of a first light beam toward a partially reflecting plate of dimensions suitable for the dimensions of the light beam of large dimensions, the dispatching onto a convergent reflective element of a second light beam arising from the transmission through the partially reflecting plate of the first light beam, the dispatching of a third light beam arising from the reflection on the convergent reflective element of the second light beam, toward said partially reflecting plate, and the reflection of the third beam on the partially reflecting plate so as to form a fourth light beam.

Abstract: The drug evaluation device obtains, by an attenuated reflection method using a terahertz wave, an evaluation absorption spectrum for a frequency with respect to a liquid to be evaluated. When crystalline particles are suspended in a liquid, an absorption peak having a peak area corresponding to the amount of suspension appears in its absorption spectrum. Therefore, whether or not and by what ratio crystalline particles are suspended in the liquid can be determined according to whether or not the absorption peak exists and the peak area. When amorphous particles are suspended in the liquid, the baseline of its absorption spectrum lowers according to the ratio of amorphous particles suspended in the liquid. Therefore, whether or not and by what ratio amorphous particles are suspended in the liquid can be determined according to the lowering amount of the baseline.

Abstract: Provided is an observation device which can obtain a phase image of a moving object rapidly with high sensitivity even when using a photodetector having a slow read-out speed per pixel. The observation device 1 comprises a light source 10, a first modulator 20, a second modulator 30, a lens 40, a beam splitter 41, a photodetector 46, and an arithmetic unit 50. The lens 40 receives scattered light generated by a moving object 2 and forms a Fourier transform image of the object 2. The photodetector 46 outputs data representing a sum in a v direction of data temporally changing at a frequency corresponding to a Doppler shift frequency of the light having reached each position on a light-receiving surface through the lens 40 at each position in a u direction at each time. The arithmetic unit 50 obtains an image of the object 2 according to the output of the photodetector 46.

Abstract: Provided is an observation device which can obtain a phase image of a moving object rapidly with high sensitivity even when using a photodetector having a slow read-out speed per pixel. The observation device 1 comprises a light source 10, a first modulator 20, a second modulator 30, a lens 40, a beam splitter 41, a photodetector 46, and an arithmetic unit 50. The lens 40 receives scattered light generated by a moving object 2 and forms a Fourier transform image of the object 2. The photodetector 46 outputs data representing a sum in a v direction of data temporally changing at a frequency corresponding to a Doppler shift frequency of the light having reached each position on a light-receiving surface through the lens 40 at each position in a u direction at each time. The arithmetic unit 50 obtains an image of the object 2 according to the output of the photodetector 46.

Abstract: The drug evaluation device obtains, by an attenuated reflection method using a terahertz wave, an evaluation absorption spectrum for a frequency with respect to a liquid to be evaluated. When crystalline particles are suspended in a liquid, an absorption peak having a peak area corresponding to the amount of suspension appears in its absorption spectrum. Therefore, whether or not and by what ratio crystalline particles are suspended in the liquid can be determined according to whether or not the absorption peak exists and the peak area. When amorphous particles are suspended in the liquid, the baseline of its absorption spectrum lowers according to the ratio of amorphous particles suspended in the liquid. Therefore, whether or not and by what ratio amorphous particles are suspended in the liquid can be determined according to the lowering amount of the baseline.

Abstract: An advanced method of and apparatus for manipulating electromagnetic spectra, which incorporates a bent tepee or bent pyramidal aligned array of conical or pyramidal inverted sections that have at least two intrinsic angles of differing values aligned co-axially. These are arranged to naturally produce a reference and object waves that impinges on and illuminate a holographic plate or recording means to produce on-axis or in-line transmission and reflection holograms, including real time display. The technology is also applicable to the detection, identification, and/or decoding of genetic material, specifically DNA and the Human Genome.

Abstract: A variable near-null compensator for measuring aspheric surfaces by subaperture stitching includes a pair of counter-rotating CGH phase plates, each of the phase plates having a phase function including two terms Z5 and Z7 of Zernike polynomials. The phase plates are mounted on a pair of precision rotary center-through tables, wherein rotational axes of the pair of precision rotary center-through tables coincide with the optical axes of the phase plates. A figure metrology apparatus includes a wavefront interferometer, the test mirror mount, the near-null compensator and the mechanical adjustment components therefor. The optical axis of the near-null compensator coincides with the optical axis of the interferometer. A method for measuring aspheric surfaces by subaperture stitching includes the steps of mounting the test mirror, measuring the subapertures with the figure metrology apparatus, and finally processing the data by stitching.

Abstract: A compact optical mount for adjust optical component in 3 dimensions. Based on the traditional 2D adjustable optical mount, its inner space is utilized for installing the third dimension angular adjustment. By virtue of its compact structure, convenient adjustment, high precision and good stability, the invention is suitable for adjusting small optics in 3 dimensions, by which the real optical axil can agree with the idea optical axil well to fast adjust the whole optical system.

Abstract: An interferometric method for detecting information about a sample includes emitting a laser beam; splitting the laser beam into a reference beam and an object beam; transmitting the object beam through the sample in an incident angle; combining the reference beam with the object beam passed through the sample to form an interference pattern; detecting the interference pattern, and non-linearly scanning the object beam in order to detect a plurality of interference patterns.

Abstract: Methods and apparatuses provide holographic contact-free imaging of aerosol particles in an efficient manner. One apparatus for holographic imaging of an aerosol particle may include: a delivery device configured to deliver the particle into a region; a light source for outputting a first beam of light and a second beam of light, wherein the first beam travels into the region producing a first light wave which is un-scattered by the particle and a second light wave that is scattered by the particle, and the second beam does not travel into the region; a beam splitter for combining the second beam with the scattered light of the first beam into combined interference light; an image sensor for sensing an interference pattern created by the combined interference light; and an image processor configured to generate an image of the aerosol particle based on the sensed interference pattern.

Abstract: A tire inspection machine includes an incoming conveyor adapted to transport tires to the machine and also align the tires in a transverse direction. An inspection table of the machine includes a table conveyor system that is responsive to a controller. The table and table conveyor are together arranged to permit scanning of both sidewall portions of a tire disposed thereon. A sensor detects a longitudinal position of the tire and stops the table conveyor when the tire is longitudinally aligned. A controller operates the table conveyor based on the longitudinal position of the tire.

Abstract: In-line holography to create images of a specimen, such as one or more particles dispersed in a transparent medium. Analyzing these images with results from light scattering theory yields the particles' sizes with nanometer resolution, their refractive indexes to within one part in a thousand, and their three dimensional positions with nanometer resolution. This procedure can rapidly and directly characterize mechanical, optical and chemical properties of the specimen and its medium.

Abstract: Reference cell (8) for use with a fiber optic probe (1) comprising a base wall (11), upstanding walls (12) each having an optical window (13, 15), and a top wall (14) having a further optical window (16) adapted to allow light to pass to and from a fiber optic probe. The base wall (11) comprises a reflector (19) which reflects incident light from the fiber optic probe back to said probe (1). The top wall (14) has an attachment for receiving an emitting and a receiving end of a fiber optic probe (1). The cell (8) allows use of standard fiber optic probe (1) with other optical equipment when the path length and the probe optical characteristics require validation to international standards. The calibration of the probe (1) is analogous to calibration of laboratory spectrophotometers and can therefore be validated. The device (8) enables probes (1) to be used for applications where precision and accuracy are essential.

Abstract: The invention relates to a method and arrangement for short coherence holography for distance measurement, for profile detection and/or for 3D detection of one or more object elements and/or object areas and/or objects or for readout of holographic volume memories with a holographic interferometer and with at least one short coherence light source. For each optically detected object element in the hologram the holographic interferometer has an optical path difference clearly unequal to zero. At least one spectrally integrally detecting, rastered detector is arranged. The short coherence light source with frequency comb is designed with the optical delay length Y1. Detected holograms are digitally reconstructed. Relative distances of object elements are digitally calculated from the hologram reconstructions, so that a 3D point cloud of object elements and/or object areas and/or objects is produced.

Abstract: A volume hologram includes: an incidence surface onto which coherent light having a plurality of wavelengths is incident; and a plurality of interference fringes having streaks so that the angles between the streaks and the incidence surface are different from each other and so that the each interference fringe corresponds to one wavelength of the coherent light.

Abstract: An optical system and associated method enable near real time optical phase conjugation. In the method, a translucent medium is illuminated by a sample illumination beam. Light scattered by the medium is directed to an electronic image sensor while a reference beam is also directed to the electronic image sensor. The scattered light and the reference beam form an interference pattern at the electronic image sensor. A digital representation of the interference pattern is recorded using the electronic image sensor, and the characteristics of a conjugate of the sample beam are computed from the numerical representation. A conjugate beam having the computed characteristics is generated using a configurable optical element and directed back to the translucent medium. The generation of the conjugate beam may be accomplished using a spatial light modulator.

Abstract: Embodiments of the invention allow the operation of confocal microscopes with relatively open pinholes (e.g. 1 Airy unit) whilst still giving a significant XY resolution improvement. In addition axial (Z) discrimination or resolution may also be improved. This is achieved by splitting the emitted light path in an interferometric fashion. One of the split beams is then directed to an image transformation system, which may perform an image inversion which inverts at least one coordinate in image space. The transformed beam and the non-transformed beam are then recombined in an interferometric fashion (i.e. coherently added), which provides an interference effect resulting in increased resolution of the image. Where the embodiments are being used in a confocal application, the resulting combined beam can then be subject to a spatially discriminating means, such as a pinhole, or the like.

Abstract: An interferometer for optically measuring an object (10), including a light source (1), at least one beam splitter (2) and at least one detector (12a, 12b), with the beam splitter being arranged in the beam path of the light source such that a light beam created by the light source is split into a working beam (3) and a reference beam (4). The interferometer is embodied such that the working beam impinges on the object (10) to be measured and the working beam is at least partially reflected by the object and interfered with the reference beam on the detector (12a, 12b).

Abstract: A method for detecting a three dimensional object smaller than 300 nm includes providing a light source producing a first light beam (1), the light source being at least partially coherent; and splitting the first light beam (1) into an object beam (2) and a reference beam (6) by a first beam splitter (BS1). An image of the light source is produced in a light source image plane by a first microscope objective (L1), in the optical path of the object beam (2). The three dimensional object to be detected in an object cell (3) is positioned in the optical path of the object beam (2), between the first beam splitter (BS1) and the first microscope objective (L1). The object beam (2) and the reference beam (6) are recombined into a recombined beam (8) by use of optical devices. An optical stop (4) is placed in the light source image plane of the microscope objective (L1) on the optical axis of the microscope objective (L1).

Abstract: A non-destructive method and device for analyzing a sample comprising transparent living and/or dead cells, by 5 means of a digital holographic microscope, where the sample (8) is exposed to light from a laser (2). The light that travels through the cells in the sample will experience a difference in the optical path length compared to the surrounding media and the wave front that emerges from the 10 cells will thus be phase shifted. This distortion can be detected in the digital hologram, which is reconstructed from the interference pattern detected by a digital sensor (17), such as a CCD or a CMOS, as phase differences or phase shifts and thereby creating a digital hologram. The 15 phase shift of each element of the hologram is then used for analyzing the characteristics of the cells in the sample.

Abstract: The present invention provides for a digital holographic microscope using a holographic interferometer and incorporating a TIR sample mount and microscopic imaging optics. The microscope uses phase shifting from frustrated internal reflection within a prism to measure nanometric distances. The invention also provides for a numerical reconstruction algorithm of an inclined surface of the object/prism.

Abstract: Disclosed are systems and methods for characterizing a nonlinear propagation environment by numerically propagating a measured output waveform resulting from a known input waveform. The numerical propagation reconstructs the input waveform, and in the process, the nonlinear environment is characterized. In certain embodiments, knowledge of the characterized nonlinear environment facilitates determination of an unknown input based on a measured output. Similarly, knowledge of the characterized nonlinear environment also facilitates formation of a desired output based on a configurable input. In both situations, the input thus characterized and the output thus obtained include features that would normally be lost in linear propagations. Such features can include evanescent waves and peripheral waves, such that an image thus obtained are inherently wide-angle, farfield form of microscopy.

Abstract: A method of forming an image of a sample includes performing SOCT on a sample. The sample may include a contrast agent, which may include an absorbing agent and/or a scattering agent. A method of forming an image of tissue may include selecting a contrast agent, delivering the contrast agent to the tissue, acquiring SOCT data from the tissue, and converting the SOCT data into an image. The contributions to the SOCT data of an absorbing agent and a scattering agent in a sample may be quantified separately.

Abstract: A non-destructive method and device for analyzing a sample comprising transparent living and/or dead cells, by 5 means of a digital holographic microscope, where the sample (8) is exposed to light from a laser (2). The light that travels through the cells in the sample will experience a difference in the optical path length compared to the surrounding media and the wave front that emerges from the 10 cells will thus be phase shifted. This distortion can be detected in the digital hologram, which is reconstructed from the interference pattern detected by a digital sensor (17), such as a CCD or a CMOS, as phase differences or phase shifts and thereby creating a digital hologram. The 15 phase shift of each element of the hologram is then used for analyzing the characteristics of the cells in the sample.

Abstract: A method of manufacturing an optical system having plural optical elements mounted relative to each other on a mounting structure of the optical system comprises disposing the optical system in a beam path of an interferometer apparatus having an interferometer optics and a selectable hologram for shaping a beam of measuring light to be incident on surfaces of the optical elements of the optical system; selecting a first hologram of the interferometer apparatus and recording at least one first interference pattern generated by measuring light reflected from a surface of a first optical element; selecting a second hologram of the interferometer apparatus, wherein the second hologram is different from the first hologram, and recording at least one second interference pattern generated by measuring light reflected from a surface of a second optical element, which is different from the first optical element; and adjusting a position of the first optical element relative to the second optical element on the mounti

Abstract: A confocal scanning holography microscope for use with a suitably selected detector for providing three dimensional information on the state of an object. The microscope has a coherent wavelength source for producing a coherent beam, scanning means for moving said coherent beam in a suitably selected pattern, and means for producing and focusing an object beam and a reference beam to an object focal point and a reference focal point, respectively. The object beam has a transmission path of essentially the same length of a transmission path of the reference beam. The object beam intercepts an object at the object focal point while the reference beam passes by the object. There are also means for defining the object beam and the reference beam based on the position of the object focal point and the geometry of the convergence angles and means for producing an interference pattern between the object focal point and the reference focal point.

Abstract: An advanced method of and apparatus for manipulating electromagnetic spectra, which incorporates a bent tepee or bent pyramidal aligned array of conical or pyramidal inverted sections that have at least two intrinsic angles of differing values aligned co-axially. These are arranged to naturally produce a reference and object waves that impinges on and illuminate a holographic plate or recording means to produce on-axis or in-line transmission and reflection holograms, including real time display. The technology is also applicable to the detection, identification, and/or decoding of genetic material, specifically DNA and the Human Genome.