Abstract: Provided is a method of constructing a sequencing library. The method includes 1) providing a single-stranded DNA fragment from a biological sample; 2) subjecting the single-stranded DNA fragment to whole genomic amplification to obtain a whole genome amplification product; 3) fragmenting the whole genome amplification product using a transposase embedded with two adaptors to obtain a fragmented product with two adaptors respectively at two ends; and 4) amplifying the fragmented product with two adaptors respectively at two ends using a tag sequence and a pair of primers to obtain said sequencing library.

Abstract: The present invention provides methods and systems for sequencing long nucleic acid fragment. The present invention also provides a method of sequencing a target polynucleotide with fewer probes. Further, the present invention provides a method of sequencing a target polynucleotide with longer reads. Locus-specific, ligation-assisted sequencing/genotyping method and ligation-captured sequencing method are also provided in the present invention. The methods of the present invention allow low-cost, high-throughput and accurate sequencing of nucleic acids.

Abstract: The present invention provides methods for targeted sequencing of polynucleotide. In one aspect, the present invention provides a method of sequencing a target polynucleotide with fewer probes. In another aspect, the present invention provides a method of sequencing a target polynucleotide with longer reads. Locus-specific, ligation-assisted sequencing/genotyping method and ligation-captured sequencing method are also provided in the present invention. The methods of the present invention allow low-cost, high-throughput and accurate sequencing of nucleic acids.

Abstract: A discharge valve apparatus of the present invention includes: a discharge portion main body member attached to discharge opening, forming the inside of discharge flow path for guiding flush water from an inflow opening formed at one end thereof to an outflow opening formed at the other end thereof; a valve body for opening and closing the inflow opening of the discharge portion main body member; and an affixing portion, formed to project into the discharge flow path of a discharge portion main body member, for affixing the discharge portion main body member to a reservoir tank; wherein the inside surface of side wall portion in the discharge portion main body member at the same height as this affixing portion forms a curved portion, which curves outward so as to expand the cross-sectional area of the flow path.

Abstract: Various methods and systems are provided for external float extensions. In one embodiment, an external float extension includes an external adjustment rod configured to affix to a quick flush float of a dual flush assembly and an external float configured to be positioned on the external adjustment rod. In another embodiment, a dual flush assembly includes a quick flush float, an external adjustment rod affixed to the quick flush float, and an external float positioned on the external adjustment rod.

Abstract: A system for microbially deactivating items. The system includes a circulation system for circulating a microbial decontamination fluid through a deactivation chamber that forms a part of the circulation system. A container for holding items to be deactivated is provided. The container has a sealable cavity in which the items to be deactivated may be placed, and at least one valve element that is movable between an open and a closed position to regulate fluid flow into the cavity. An actuator in the deactivation chamber is operable to move the valve element to an open position when the container is disposed within the deactivation chamber, wherein the cavity within the container is in fluid communication with the circulation system when the valve element is in the open position.

Abstract: A pressurized water closet flushing system for flushing a toilet includes a water vessel having at least one opening for receiving water. A fill tube and a discharge tube extending into the vessel are secured to the vessel. A modular assembly cap including an intake and an actuator covers the opening. The fill tube remains secured to the vessel when the assembly cap is removed from the vessel.

Abstract: Disclosed are novel methods and compositions for rapid and highly efficient nucleic acid sequencing based upon hybridization with two sets of small oligonucleotide probes of known sequences. Extremely large nucleic acid molecules, including chromosomes and non-amplified RNA, may be sequenced without prior cloning or subcloning steps. The methods of the invention also solve various current problems associated with sequencing technology such as, for example, high noise to signal ratios and difficult discrimination, attaching many nucleic acid fragments to a surface, preparing many, longer or more complex probes and labelling more species.

Abstract: A flush valve device is described for use in a toilet that includes a pressured water source, which can drain away water in the event of excess pressure with minimal modification to the toilet and which facilitates initial operation of the flush valve device. The flush valve device includes a valve member (16, FIG. 2 ) which lifts off a valve seat (22) at the beginning of a flushing, the valve member carrying a pressure operated relief valve (50) which dumps water at excess pressure through the opening (52) within the valve seat, to avoid the need for additional fluid-tight couplings in the water source. The valve member includes a sealing portion (60) which normally lies against the valve seat and which is lifted completely away from the valve seat during a flushing.

Abstract: Savings in water consumption are obtained and problems with leakage are avoided by using a water-closet valve which is made of flexible air- and water-tight material and has not only a generally frustoconical seat-engaging portion provided with a pair of circumferential rings or protuberances so as to permit the formation of a double seal but also an interior hollow portion such that towards the end of a flushing operation there is produced within the valve member a partial vacuum which creates a thrust force to perfect the sealing of the valve member within its seat.

Abstract: A water toilet has a valve arrangement of the integrated type, i.e. the float and all valves form a unit which in its mounted position is connected to the outlet pipe of the flush water container. According to the invention the unit is loosely connected to said pipe and mechanically retained at its top end by means cooperating with the inner surface of the top portion of the water container wall.

Abstract: An improved water closet valve is provided having two separate and distinct circular sealing rings interposed between the valve bulb and the conical opening which constitutes the valve seat. Both sealing rings may be secured to the bulb; both may be secured to the conical opening or one may be secured to the valve bulb and another to the surface of the conical opening. When the valve is in a closed position, at least two distinct spaced-apart seals are provided to obstruct the flow of water.

Abstract: A pressure flush tank assembly for toilets which includes a combination of a flush valve coordinated with an air induction valve and refill valve to insure successful repetitive operation, together with a construction which prevents back-flow in the event of a negative line pressure and an initial outflow control to reduce noise and eliminate back bounce from the toilet bowl.