Abstract: This invention relates to a method for screening for a growth-promoting factor for pluripotent stem cells with a conditioned medium which is generated by culturing feeder cells in a serum-free medium that contains L-ascorbic acid, insulin, transferrin, selenium, and sodium bicarbonate and does not contain a serum nor serum replacement; a method for growing pluripotent stem cells via feeder-free culture using the conditioned medium; and a method for growing pluripotent stem cells by carrying out feeder-free culture of pluripotent stem cells cultured in advance on feeder cells in the serum-free medium.

Abstract: An apparatus (10) for and a method of transporting sliders to the slider insertion area (27) of a slider insertion device (18). A first sensor (30) on a sender track (20) detects a lack of sliders (21) in the sender track. A signal is sent from the first sensor (30) to a controller (24) to actuate a vibratory bowl (14) of sliders (21), and a first solenoid operated plunger (34) thereby releasing the sliders (21) to the sender track (20). Air connections (38) pneumatically push the sliders (21) along the sender track (20) to a second solenoid-operated plunger (44). When a second sensor (46) detects a lack of sliders in the loading rack (26) , the second sensor (46) signals the controller (24) to actuate a second solenoid-operated plunger (44) and a solenoid-operated pneumatic valve (48). The second solenoid-operated plunger (44) opens a passage and the pneumatic valve (48) opens to provide an air blast, thereby launching the sliders (21) into the feed tube (22) and onto the slider insertion area (27).