Abstract: A fastening apparatus for a vehicle may include: first fastener fixed to a first structure, a case fixed to the second structure and configured to have the first fastener inserted into an one side of the case, an elevation unit installed within the case in a way to be movable up and down, a second fastener rotatably supported against the elevation unit, fastened to or separated from the first fastener in a rotation direction thereof, and moved up and down along with the elevation unit, and a locking unit configured to interfere with a work tool that enters an another side of the case and to selectively permit the rotation of the second fastener.