Abstract: This invention discloses means for obtaining immunogenic peptides, polypeptides, proteins, and their corresponding nucleic acid sequences, target cells with vaccine interest, or lysates thereof, without making structural changes in said antigens, through their association with Very Small Size Proteoliposomes.
Type:
Application
Filed:
December 6, 2001
Publication date:
September 26, 2002
Inventors:
Luis Enrique Fernandez Molina, Belinda Sanchez Ramirez, Eduardo Raul Suarex Pestana, Anabel de la Barrera Aira, Circe Mesa Pardillo, Joel de Leon Delgado, Yildian Diaz Rodriguez, Rolando Perez Rodriguez
Abstract: Disclosed is an H. influenzae type b polysaccharide-meningococcal outer membrane protein conjugate, pharmaceutical compositions thereof, and the use thereof to induce an immune response to H. influenzae in an animal.
Type:
Grant
Filed:
July 17, 1998
Date of Patent:
September 17, 2002
Assignee:
Baxter International Inc.
Inventors:
Milan S. Blake, Francis Michon, Peter C. Fusco, Iver Heron
Abstract: The present invention generally provides methods and vaccines for the prevention of diseases caused by Neisseria meningitidis bacteria, particularly serogroup B strains.
Abstract: An isolated and purified lactoferrin receptor protein is isolated and purified from bacterial pathogens, including Moraxella and Neisseria, and has a molecular weight of between about 70,000 and about 90,000, as determined by SDS-PAGE. Such lactoferrin receptor protein may be provided in combination with a lactoferrin receptor protein from the bacterial pathogen of a molecular weight of about 100,000 to about 105,000 daltons. The lactoferrin receptor protein may be produced by providing a solubilized membrane preparation from the bacterial pathogen containing lactoferrin receptor proteins, non-lactoferrin receptor proteins and other contaminants, complexing the lactoferrin receptor proteins with lactoferrin and purifying the resulting complexes substantially free from the non-lactoferrin receptor proteins and the other contaminants, and separating the novel lactoferrin receptor protein from the complexes.
Abstract: The present invention relates to a gene sequence coding for hybrid Protein I of Neisseria gonorrhoeae, and cloning vector and host organisms containing same. Also disclosed are oligonucleotides useful as diagnostic probes for the detection of N. gonorrhoeae infection.
Type:
Grant
Filed:
June 15, 1999
Date of Patent:
February 19, 2002
Assignee:
The University of North Carolina at Chapel Hill
Inventors:
Nicholas H. Carbonetti, P. Frederick Sparling
Abstract: An isolated and purified lactoferrin receptor protein is isolated and purified from bacterial pathogens, including Moraxella and Neisseria, and has a molecular weight of between about 70,000 and about 90,000, as determined by SDS-PAGE. Such lactoferrin receptor protein may be provided in combination with a lactoferrin receptor protein from the bacterial pathogen of a molecular weight of about 100,000 to about 105,000 daltons. The lactoferrin receptor protein may be produced by providing a solubilized membrane preparation from the bacterial pathogen containing lactoferrin receptor proteins, non-lactoferrin receptor proteins and other contaminants, complexing the lactoferrin receptor proteins with lactoferrin and purifying the resulting complexes substantially free from the non-lactoferrin receptor proteins and the other contaminants, and separating the novel lactoferrin receptor protein from the complexes.
Abstract: Multivalent immunogenic molecules comprise a carrier molecule containing at least one functional T-cell epitope and multiple different carbohydrate fragments each linker to the carrier molecule and each containing at least one functional B-cell epitope. The carrier molecule inputs enhanced immunogenicity to the multiple carbohydrate fragments. The carbohydrate fragments may be capsular oligosaccharide fragments from Streptococcus pneumoniae, which may be serotypes 1, 4, 5, 6B, 9V, 14, 18C, 19F or 23F, or Neisseria meningitidis, which may be serotype A, B, C, W-135 or Y. Such oligosaccharide fragments may be sized from 2 to 5 kDa. Alternatively, the carbohydrate fragments may be fragments of carbohydrate-based tumor antigens, such as Globo H, LeY or STn. The multivalent molecules may be produced by random conjugation or site-directed conjugation of the carbohydrate fragments to the carrier molecule.
Abstract: Bacterial antigen preparations for use as live or killed immunogens and vaccines can be produced by culture of bacteria such as Neisseria in medium comprising norepinephrine or other catechol-group-containing growth inducer of bacterial growth, harvesting and pharmaceutical formulation. The antigen preparations can comprise bacterial protein(s) inducible by norepinephrine.
Type:
Application
Filed:
December 18, 2000
Publication date:
October 18, 2001
Inventors:
Thomas John Baldwin, Paul Williams, Simon Swift, Cornelia Suzanna McLean, Christopher Lee Holmes
Abstract: The present disclosure provides methods and compositions for inducing an immune response to an antigen, especially in an immunogenic composition comprising sialic acid where the antigen comprises sialic acid and wherein the immunogenic composition further comprises a sialic acid binding component, e.g., an inactivated or attenuated paramyxovirus or orthomyxovirus such as an influenza virus comprising a sialic acid binding component, e.g., a neuraminidase. The compositions comprising sialic acid and a sialic acid binding component effectively induce a humoral immune response even in a human or animal which is deficient in CD4+ T cells, due to a disease such as ARC or AIDS, and there is also an immunoglobulin class switching even in the absence of CD4+ T cells.
Abstract: A highly conserved, immunologically accessible antigen at the surface of Neisseria meningitidis organisms. Immunotherapeutic, prophylactic and diagnostic compositions and methods useful in the treatment, prevention an diagnosis of Neisseria meningitidis diseases. A proteinase K resistant Neisseria meningitidis surface protein having an apparent molecular weight of 22 kDa, the corresponding nucleotide and derived amino acid sequences (SEQ ID NO: 1, NO:3, NO:5 and NO:7: SEQ ID NO: 2, NO:4, NO:6, and NO:8), recombinant DNA methods for the production of the Neisseria meningitidis 22 kDA surface protein, and antibodies that bind to the Neisseria meningitidis 22 kDA surface protein.
Type:
Grant
Filed:
November 13, 1997
Date of Patent:
September 11, 2001
Assignee:
BioChem Pharma Inc.
Inventors:
Bernard R. Brodeur, Denis Martin, Josee Hamel, Clement Rioux
Abstract: The present invention relates to novel bacterial hemoglobin receptor proteins and genes that encode such proteins. The invention is directed toward the isolation, characterization, diagnostic and therapeutic use of bacterial hemoglobin receptor proteins, nucleic acids encoding such proteins, recombinant expression constructs comprising such nucleic acids and cells transformed therewith, and antibodies and epitopes of such hemoglobin receptor proteins. The invention relates particularly to hemoglobin receptor proteins and genes encoding such proteins from Neisseria species, especially N. meningitidis and serotypes thereof, and N. gonorrhoeae. Methods for the diagnostic and therapeutic use of the proteins, epitopes, antibodies and nucleic acids of the invention are also provided, including the use of proteins, epitopes, antibodies and nucleic acids of the invention for the production of vaccines effective in providing immunization of human against infection by pathogenic bacteria of Neisseria species.
Type:
Grant
Filed:
August 19, 1997
Date of Patent:
August 21, 2001
Assignee:
Oregon Health Sciences University
Inventors:
Igor Stojiljkovic, Magdalene So, Vivian Hwa, Fred Heffron, Xavier Nassif
Abstract: A novel composition comprising Invaplex from gram-negative bacteria is described and is effective as a vaccine against gram-negative bacterial infection.
Type:
Grant
Filed:
September 29, 1999
Date of Patent:
August 21, 2001
Assignee:
The United States of America as represented by the Secretary
of the Army
Inventors:
Edwin V. Oaks, Kevin Ross Turbyfill, Antoinette Berrong Hartman
Abstract: A method for inducing and enhancing mammalian mucosal immunity includes the steps of first administering to a mammal via a non-mucosal route a suitable amount of a priming vaccine composition which comprises a DNA sequence encoding an antigen of a pathogen under the control of regulatory sequences directing expression thereof in a mammalian cell, and subsequently, administering intranasally a boosting vaccine composition which comprises the same antigen in protein form or a DNA sequence encoding the same antigen.
Type:
Grant
Filed:
August 19, 1999
Date of Patent:
April 3, 2001
Assignee:
The Wistar Institute of Anatomy and Biology
Abstract: An isolated and purified lactoferrin receptor protein is isolated and purified from bacterial pathogens, including Moraxella and Neisseria, and has a molecular weight of between about 70,000 and about 90,000, as determined by SDS-PAGE. Such lactoferrin receptor protein may be provided in combination with a lactoferrin receptor protein from the bacterial pathogen of a molecular weight of about 100,000 to about 105,000 daltons. The lactoferrin receptor protein may be produced by providing a solubilized membrane preparation from the bacterial pathogen containing lactoferrin receptor proteins, non-lactoferrin receptor proteins and other contaminants, complexing the lactoferrin receptor proteins with lactoferrin and purifying the resulting complexes substantially free from the non-lactoferrin receptor proteins and the other contaminants, and separating the novel lactoferrin receptor protein from the complexes.
Abstract: The invention relates to a hyperblebbing strain of Neisseria gonorrhoeae which produces large amounts of blebs useful for production of blebosomes containing antigens for use as a vaccine delivery vehicle or as a diagnostic reagent. The invention also relates to a method for producing high levels of a desired protein in purified form using the hyperblebbing strain of N. gonorrhoeae, and to a vaccine delivery systems containing the blebosomes expressing the desired antigen.