Maintaining Blood Or Sperm In A Physiologically Active State Or Compositions Thereof Or Therefor Or Methods Of In Vitro Blood Cell Separation Or Treatment Patents (Class 435/2)
  • Patent number: 11583620
    Abstract: The present invention generally relates to a process suitable for extracorporeal lung support. The process comprises contacting blood with a dialysis liquid separated by a semipermeable membrane. Oxygen is introduced into blood and/or into the dialysis liquid prior to contacting blood and dialysis liquid being separated by the semipermeable membrane. The process is versatile and allows for blood oxygenation as well as removal of at least one undesired substance occurring in the blood, selected from carbon dioxide, bicarbonate and hydrogen cations, from blood. Thereby, the present invention takes advantage of the Haldane effect in the extracorporeal contacting step. The undesired substance can be efficiently transported across a semipermeable membrane to the dialysis liquid.
    Type: Grant
    Filed: November 18, 2016
    Date of Patent: February 21, 2023
    Assignee: Advitos GmbH
    Inventors: Bernhard Kreymann, Christoph Hüßtege
  • Patent number: 11579153
    Abstract: A diagnostic biological array, kit or system, and method of using same unit for conducting simultaneously blood tests and determining the presence of diseases, the blood type, and blood quality of a blood sample and its applications.
    Type: Grant
    Filed: August 17, 2017
    Date of Patent: February 14, 2023
    Inventor: Arie Huber
  • Patent number: 11554185
    Abstract: Provided are systems and methods for treating a biological fluid, e.g., to inactivate pathogens.
    Type: Grant
    Filed: December 28, 2018
    Date of Patent: January 17, 2023
    Assignee: Cerus Corporation
    Inventors: Daniel Church, Peter Bringmann, Grace Castro, Thea Lu, Shelby Reinhardt, Felicia Santa Maria, Adonis Stassinopoulos
  • Patent number: 11547708
    Abstract: A composition for preserving viability of cells, tissues, or organs at a low temperature is provided. The composition includes a mitochondrial uncoupling agent, at least one protease inhibitor, and a reducing agent. Methods to protect cells, tissues, or organs from exposure to cold and other metabolic stress are also provided.
    Type: Grant
    Filed: August 20, 2018
    Date of Patent: January 10, 2023
    Inventors: Jingxing Ou, Wei Li, Kiyoharu Josh Miyagishima
  • Patent number: 11529587
    Abstract: Provided herein are materials and methods for the preparation of blood products. In one aspect, provided herein is a composition including platelets or platelet derivatives and an aqueous medium, wherein the aqueous medium has a protein concentration less than 50% of the protein concentration of donor apheresis plasma.
    Type: Grant
    Filed: May 1, 2020
    Date of Patent: December 20, 2022
    Assignee: Cellphire, Inc.
    Inventors: Joshua Donald Montgomery, Braden Carl Ishler, Stephen Edward Amos, Keith Andrew Moskowitz, Amber Nicole Lee, Rafael Jorda, Glen Michael Fitzpatrick, Michael Alexander Mathews
  • Patent number: 11523606
    Abstract: A system and method are provided for wet storage of tissue. In an embodiment, a solution for the wet preservation of tissue may include between about 0.1% to about 50% by volume dimethyl sulfoxide (DMSO) and one or more soluble monovalent or divalent metal cationic salts. A wet-preserved tissue and method for preparing the wet-preserved tissue for ultimate use, is also provided.
    Type: Grant
    Filed: July 27, 2020
    Date of Patent: December 13, 2022
    Assignee: Axogen Corporation
    Inventor: Mindy E. Sadik
  • Patent number: 11511026
    Abstract: Peritoneal dialysis, such as automated peritoneal dialysis (“APD”) is provided with any one or more or all of the following sensing or feedback features: impedance sensing to detect peritonitis, temperature sensing to detect peritonitis, bio-MEMS sensing to detect peritonitis, and glucose control for diabetes patients, wherein each sensing or feedback feature analyzes patient effluent fluid or fluid dwelling within a patient's peritoneal cavity.
    Type: Grant
    Filed: July 25, 2019
    Date of Patent: November 29, 2022
    Assignees: Baxter International Inc., Baxter Healthcare SA
    Inventors: Sukhraaj Basati, Shantanu Avinash Gadre, Mohammad Ali Jamnia, Lakshmy M. Nair, William J. O'Reilly, Marta Wlodarczyk
  • Patent number: 11485953
    Abstract: The present disclosure provides for a cell stabilizing medium which comprises gelatin. The cell stabilizing medium help maintain cell viability, e.g., after thawing of a biological material post-cryopreservation.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: November 1, 2022
    Inventors: Ya-Hsuan Chang, Cheng-Yi Lin, Chih-Yuan Chao
  • Patent number: 11455719
    Abstract: A method of image-based analysis of multiple samples includes using a sample holder having multiple locations of interest and multiple focal structures that are each associated, one or more, with the multiple locations of interest, wherein the multiple samples are dispersed across the multiple locations of interest and obtaining image areas of the multiple locations of interest. Multiple digital image areas are thus obtained for use in an analysis of the multiple samples with each of the image areas including at least one of the locations of interest and at least one of the focal structures. An image processing algorithm is used to analyse each of the digital image areas and check if the focal structure indicates that the image area is in clear focus. An indication is provided and/or remedial action is taken if the image processing algorithm indicates that any digital image areas are out of focus.
    Type: Grant
    Filed: June 15, 2017
    Date of Patent: September 27, 2022
    Assignee: Q-LINEA AB
    Inventors: Jonas Jarvius, Jan Grawe
  • Patent number: 11433164
    Abstract: A system and methodology for the preservation of red blood cells is described in which red blood cells are oxygen or oxygen and carbon dioxide depleted, treated and are stored in an anaerobic environment to optimize preparation for transfusion. More particularly, a system and method for extended storage of red blood cells from collection to transfusion that optimizes red blood cells prior to transfusion is described.
    Type: Grant
    Filed: February 7, 2020
    Date of Patent: September 6, 2022
    Assignee: Hemanext Inc.
    Inventors: Tatsuro Yoshida, Paul Vernucci
  • Patent number: 11433173
    Abstract: The present invention relates to a system (100) for extracorporeal blood treatment comprising a first inlet (1) for introducing a bloodstream to be treated into the system (100), three blood treatment apparatus (A, D, G), as well as an outlet (2) for discharging a treated bloodstream from the system (100), wherein the system comprises an adsorber apparatus (A) and/or a plasma separator apparatus, a dialysis apparatus (D) and a gas exchange apparatus (G), and wherein the three blood treatment apparatus (A, D, G) are sequentially connected in series in a functional state of system (100) application between the inlet (1) and the outlet (2) of the system relative to a direction of blood flow of a bloodstream to be treated and can be consecutively perfused extracorporeally by a bloodstream to be treated.
    Type: Grant
    Filed: December 14, 2017
    Date of Patent: September 6, 2022
    Inventors: Jorg Rammo, Jurgen Klewinghaus
  • Patent number: 11413358
    Abstract: The invention encompasses a rapid and safe preparation method of sperm nuclei, improved sperm nuclei and a method of using the improved sperm nuclei to calibrate a flow cytometer with higher accuracy.
    Type: Grant
    Filed: April 9, 2019
    Date of Patent: August 16, 2022
    Assignee: Inguran, LLC
    Inventors: Kenneth Michael Evans, Thomas B. Gilligan
  • Patent number: 11371983
    Abstract: A plasma separation system and process for providing filtered plasma from a blood sample is described. The system may include a blood separation well having a separation membrane for filtering the blood sample. The filtering process may be aided by the use of a negative or positive pressure source attached to the plasma separation system.
    Type: Grant
    Filed: April 9, 2021
    Date of Patent: June 28, 2022
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Michael S. Wilson, Jay Li, Kenneth P. Galano
  • Patent number: 11352615
    Abstract: The invention relates to a device (1) for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber (6) which comprises at least two electrodes (4), and at least one separating element (13) which is movable within the chamber (6) between two terminal points (14, 15) and, if it is in a position between the terminal points (14, 15), separates at least one first compartment (26) of the chamber (6) from at least one second compartment (27) of the chamber (6).
    Type: Grant
    Filed: November 9, 2018
    Date of Patent: June 7, 2022
    Assignee: Lonza Cologne GmbH
    Inventors: Ludger Altrogge, Timo Gleissner, Andreas Heinze, Sven Hermsmeier
  • Patent number: 11255841
    Abstract: Distributed liquid-flow systems—in which flow spreads out from a system inlet and traverses the system through multiple discrete, smaller flow channels—are constructed to minimize variations in flow-resistance-induced pressure drop from the system inlet to entrances to the flow channels. Because flow-driving pressure will be more uniform at the entrances to the flow channels, flow along the channels will be more uniform. Disclosed embodiments may be particularly suitable or advantageous for use in gas-exchange/artificial lung devices.
    Type: Grant
    Filed: August 1, 2019
    Date of Patent: February 22, 2022
    Assignee: Nano2cure Ltd.
    Inventor: Yoram Palti
  • Patent number: 11231425
    Abstract: An immunoassay includes forming first mixtures by reacting a combination reagent with serum of a subject; simultaneously forming second mixtures by reacting randomly selected platelet samples with the serum wherein in each mixture there are immunity compounds formed by combining the platelet antigens with predetermined antibodies in the serum, and other platelet antigens and other antibodies in the serum not forming the immunity compounds; preparing an interception device including receptacles and a filter net; placing each mixture in one receptacle; washing the mixtures wherein the mixtures forming the immunity compounds are intercepted by the filter net with others passing through; adding a signal sensing reagent to each receptacle; reacting the signal sensing reagent with the intercepted mixtures forming the immunity compounds to form final products; and performing a signal sensing to determine whether the final products contain anti-platelet antibodies and determine compatibility of cross matching of resp
    Type: Grant
    Filed: September 11, 2019
    Date of Patent: January 25, 2022
    Inventors: Tzong-Shi Chiueh, Min-Hsien Wu, Hsin-Yao Wang
  • Patent number: 11224217
    Abstract: Disclosed herein are methods and compositions for preserving avulsed teeth and/or explanted tissue. The disclosed methods for preserving avulsed teeth and/or explanted tissue typically include placing the avulsed teeth and/or explanted tissue in a preservation solution comprising at least about 2.75 g/L sugar. Suitable explanted tissues for the disclosed methods may include explanted tissues comprising fibroblasts, such as dental tissue and/or oral tissue. The preservation solution of the disclosed preservation methods may include additional components which may include but are not limited to inorganic salts, amino acids, vitamins, and buffering agents. The preservation solution may be prepared from a powder formulation having a long shelf-life.
    Type: Grant
    Filed: April 24, 2019
    Date of Patent: January 18, 2022
    Assignee: Marquette University
    Inventors: Lobat Tayebi, Morteza Rasoulianboroujeni
  • Patent number: 11197893
    Abstract: A pure platelet-rich plasma (P-PRP) composition as an alternative to conventional antibiotic treatment of subclinical mastitis caused by Gram-positive bacteria in bovine including five live platelets and leukocytes, an anticoagulant, and an activating substance.
    Type: Grant
    Filed: November 8, 2016
    Date of Patent: December 14, 2021
    Assignee: Universidad de Caldas
    Inventors: Alejandro Ceballos, Jorge U. Carmona
  • Patent number: 11191880
    Abstract: A system for processing fluids and filling a container with a product includes a disposable fluid circuit and reusable hardware configured to accept the disposable fluid circuit. The disposable fluid circuit includes a spinning membrane separator, first and second syringes, and a flow control cassette. The reusable hardware includes a drive coupled to the spinning membrane separator, first and second syringe pumps, the first and second syringes coupled to the first and second syringe pumps respectively, and a controller. The system also includes a syringe pump for filling low-volume containers, which syringe pump may be one of the first and second syringe pumps, or may be a third syringe pump. The syringe pump for filling low-volume containers may include a filtered vacuum/pressure source and a position detector.
    Type: Grant
    Filed: May 14, 2019
    Date of Patent: December 7, 2021
    Assignee: FENWAL, INC.
    Inventors: Kyungyoon Min, Christopher J. Wegener
  • Patent number: 11185068
    Abstract: Provided are a diluent and a sperm preservation method using the diluent. The diluent is useful in preservation of sperm with high fertility, and is capable of stably achieving quality control and improving quality of the sperm. Refrigerating or freezing sperm using the diluent for sperm, which includes an aqueous solution containing at least one oligosaccharide selected from the group consisting of a fructo-oligosaccharide, an isomalto-oligosaccharide, a gentio-oligosaccharide, and a galacto-oligosaccharide, can improve the quality of preserved sperm and provide sperm having high fertility, at a reduced cost.
    Type: Grant
    Filed: September 13, 2018
    Date of Patent: November 30, 2021
    Assignee: Livestock Improvement Association of Japan, Inc.
    Inventors: Masashi Kinukawa, Katsutoshi Funauchi, Kyoko Uchiyama
  • Patent number: 11166453
    Abstract: Provided herein is technology relating to cryopreservation and particularly, but not exclusively, to devices, systems, and methods for cryopreservation of biological materials such as oocytes, zygotes, and embryos. In particular, provided herein are methods of using microfluidic devices to exposing biological material to a vitrification solution having a time dependent concentration of a cryoprotectant agent.
    Type: Grant
    Filed: October 23, 2018
    Date of Patent: November 9, 2021
    Assignee: The Regents of the University of Michigan
    Inventors: David Lai, Shuichi Takayama, Gary D. Smith, Jun Ding
  • Patent number: 11090331
    Abstract: Method for transfusion medicine to reduce adverse events in transfusion patient populations based on underlying patient physiology. Methods for reducing the risk of an inflammatory response in a sickle cell patient in need of a blood transfusion.
    Type: Grant
    Filed: May 15, 2020
    Date of Patent: August 17, 2021
    Assignee: Hemanext Inc.
    Inventors: Angelo D'Alessandro, Rafael Cordero, Andrew Dunham, Philip Keegan, Tatsuro Yoshida
  • Patent number: 11077241
    Abstract: The present invention is related to the field of tissue regeneration. It concerns more particularly new standardizations and medical devices for the preparation of A-PRP, PRP, BMC, fat tissue, alone or in combination with a biomaterial or cell extract.
    Type: Grant
    Filed: November 26, 2015
    Date of Patent: August 3, 2021
    Assignee: REGEN LAB USA LLC
    Inventor: Antoine Turzi
  • Patent number: 11069813
    Abstract: A method of forming a semiconductor device includes forming source/drain contact openings extending through at least one dielectric layer to expose source/drain contact regions of source/drain structures. The method further includes forming conductive plugs in the source/drain contact openings. The method further includes depositing a light blocking layer over the conductive plugs and the at least one dielectric layer. The method further includes etching the light blocking layer to expose the conductive plugs. The method further includes directing a laser irradiation to the conductive plugs and the light blocking layer. The laser irradiation is configured to activate dopants in the source/drain contact regions.
    Type: Grant
    Filed: September 30, 2019
    Date of Patent: July 20, 2021
    Assignee: Taiwan Semiconductor Manufacturing Co., Ltd.
    Inventors: Blandine Duriez, Marcus Johannes Henricus Van Dal, Martin Christopher Holland, Gerben Doornbos, Georgios Vellianitis
  • Patent number: 11033900
    Abstract: A method for treating a blood specimen with which capturing rates for both a small rare cell and a rare cell having a high deformability can be improved in the case where rare cells are contained in a blood specimen. The method for isolating or detecting a rare cell includes treating a blood specimen using a filter to isolate or detect a rare cell in the blood specimen.
    Type: Grant
    Filed: March 23, 2016
    Date of Patent: June 15, 2021
    Assignee: ARKRAY, Inc.
    Inventor: Hidenori Takagi
  • Patent number: 11008566
    Abstract: The present invention relates to a method for obtaining an antibody from an avian B cell antibody library, comprising the following steps (a) to (d): (a) a step of allowing an avian B cell antibody library to come into contact with an antigen in the presence of a calcineurin inhibitor and avian serum, (b) a step of selecting avian B cells that bind to the antigen in the step (a), (c) a step of culturing the avian B cells selected in the step (b) in the presence of a calcineurin inhibitor and avian serum, and (d) a step of obtaining the avian B cells obtained through the step (c) and/or an antibody expressed by the avian B cells.
    Type: Grant
    Filed: September 29, 2017
    Date of Patent: May 18, 2021
    Assignee: Chiome Bioscience Inc.
    Inventors: Yosuke Kamimura, Kotaro Yamamoto
  • Patent number: 10996218
    Abstract: The present invention provides a method for determining the gender of a fetus by assaying the sex hormones, evaluating the overall reducing/oxidizing (redox) activity, and/or evaluating the radical scavenging capacity of the maternal urine or other body fluid. The method can be used to determine fetal gender at any time point during pregnancy. The methods of the present invention also provide for a means for pre-conception offspring gender planning by assaying the sex hormones in urine samples, evaluating the overall reducing/oxidizing (redox) activity, and/or evaluating the radical scavenging capacity of the urine or other body fluid from a non-pregnant female. In some embodiments, the methods described herein are used to sort sperm based on an overall reducing/oxidizing environment of follicular fluids, which can then be used for intrauterine insemination.
    Type: Grant
    Filed: June 14, 2018
    Date of Patent: May 4, 2021
    Inventor: Kuldeep C. Verma
  • Patent number: 10980460
    Abstract: The present invention relates to devices and methods of use thereof for detection of biomolecules, in vitro, in vivo, or in situ. The invention relates to methods of diagnosing and/or treating a subject as having or being at risk of developing a disease or condition that is associated with abnormal levels of one or more biomolecules including, but not limited to, inter alia, epilepsy, diseases of the basal ganglia, athetoid, dystonic diseases, neoplasms, Parkinson's disease, brain injuries, spinal cord injuries, and cancer. The invention also provides methods of differentiating white matter from gray matter. In some embodiments, regions of the brain to be resected or targeted for pharmaceutical therapy are identified using sensors. The invention further provides methods of measuring the neurotoxicity of a material by comparing microvoltammograms of a neural tissue in the presence and absence of the material using the inventive sensors.
    Type: Grant
    Filed: February 15, 2017
    Date of Patent: April 20, 2021
    Assignees: Research Foundation of The City University of New York, New York University School of Medicine
    Inventors: Patricia A. Broderick, Steven V. Pacia
  • Patent number: 10946046
    Abstract: A method for sterilising a platelet lysate in the liquid state comprising at least the endogenous growth factors TGF-beta 1, EGF, PDGF-AB, IGF-1, VEGF and bFGF. The method comprising freezing the liquid platelet lysate in order to obtain a frozen platelet lysate, and irradiating the frozen platelet lysate with ionising radiation in order to obtain a sterilised platelet lysate, the irradiation being adapted so as to preserve at least 80% of the concentration of at least one of the endogenous growth factors chosen from the group consisting of TGF-beta 1, EGF, PDGF-AB, IGF-1 and VEGF.
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: March 16, 2021
    Assignee: Maco Pharma
    Inventors: Bruno Delorme, Sabrina Viau, Francis Goudaliez
  • Patent number: 10940260
    Abstract: Provided is a blood component separation device and related method, each capable of separating a blood component from blood and rapidly performing a virus inactivation process on the separated blood component. The blood component separation device includes a blood component separation section, provided in a centrifuge, configured to separate a blood component from blood by centrifugation, a diluting section configured to dilute, in a diluent containing riboflavin, concentrated red blood cells separated by the blood component separation section, and a UV light emitting unit configured to perform a virus inactivation process by exposing the concentrated red blood cells thus diluted to the UV light.
    Type: Grant
    Filed: March 13, 2017
    Date of Patent: March 9, 2021
    Assignee: Terumo Kabushiki Kaisha
    Inventor: Masatsugu Igarashi
  • Patent number: 10935560
    Abstract: The invention described herein relates to a method and kit for the diagnosis of antiphospholipid syndrome in patients, wherein a coagulation inhibitor and normal pooled plasma mixture reduce the factor deficiency effect of patient plasma, and coagulation inhibitor interference in patient plasma, and increases specificity and the sensitivity for detecting lupus anticoagulants in the patient's blood in a phospholipid-dependent clotting assay.
    Type: Grant
    Filed: April 19, 2017
    Date of Patent: March 2, 2021
    Assignee: Instrumentation Laboratory Company
    Inventors: Ralph E. Bottenus, Zhenghua Cao
  • Patent number: 10888259
    Abstract: Disclosed herein are devices, apparatus, systems, methods and kits for collecting and storing a fluid sample from a subject. A device for collecting the fluid sample can include a housing comprising a recess having an opening, a vacuum chamber in the housing and in fluidic communication with the recess, and one or more piercing elements that are extendable through the opening to penetrate skin of the subject. The vacuum chamber can be configured for having a vacuum that draws the skin into the recess. The recess can be configured having a size or shape that enables an increased volume of the fluid sample to be accumulated in the skin drawn into the recess.
    Type: Grant
    Filed: November 15, 2019
    Date of Patent: January 12, 2021
    Assignee: Drawbridge Health, Inc.
    Inventors: Brett L. Jordan, Masao Drexel, Dagmar Beyerlein, Alicia Jackson, Kara Juneau
  • Patent number: 10850280
    Abstract: Methods, devices, and kits, related to embodiments for uniformly coating at least one inner surface of a capillary with at least one anticoagulant compound.
    Type: Grant
    Filed: November 14, 2018
    Date of Patent: December 1, 2020
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventor: Aaron Kauffmann
  • Patent number: 10815456
    Abstract: The present disclosure provides for a cryopreservation composition comprising a permeating cryoprotectant, a saccharide and a macromolecule. The cryopreservation composition has a low toxicity to cells and tissues, and promotes survival and retention of viability of the biological material during cryopreservation.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: October 27, 2020
    Inventors: Bin-Ru She, Kun-Chi Chiang, Cheng-Yi Lin, Ting-Yu Huang
  • Patent number: 10780438
    Abstract: An interfacial technique utilizes hydrodynamic micro-vortices to perform (i) high efficiency single cell encapsulation and (ii) size-selective capturing of cells based on their sizes in a single microfluidic device. A notable feature of this technique is that it can perform high efficiency single cell encapsulation at low cell concentrations, and this technique is all passive, controlled only by the flow rates of the two phases and does not require complex structures or on-chip active devices. Single bead/cell encapsulation was demonstrated at 50% efficiency, which is at least 10 times greater than the random encapsulations at the introduced cell concentrations. Also demonstrated is the selective trapping of cells based on their sizes. This present technique expands the capabilities of droplet microfluidics for applications ranging from single cell genomics, proteomic assays to sample preparation.
    Type: Grant
    Filed: June 11, 2018
    Date of Patent: September 22, 2020
    Inventors: Gopakumar Kamalakshakurup, Abraham P. Lee
  • Patent number: 10763387
    Abstract: A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.
    Type: Grant
    Filed: May 3, 2019
    Date of Patent: September 1, 2020
    Assignee: ABS Global, Inc.
    Inventors: Frederick Hershel Savage, Glenn J. Szejna, Zheng Xia
  • Patent number: 10750739
    Abstract: Methods for stabilizing blood samples, e.g., clinical blood samples, for storage or transportation before use. Exemplary applications include but are not limited to enrichment of leukocyte subtypes such as T-cells or neutrophils for cytokine and immuno-assays; isolation of progenitor cells from cord blood or peripheral blood for transplantation; isolation of fetal cells from the maternal blood for diagnosis; and sorting of circulating tumor cells for cancer detection and therapy.
    Type: Grant
    Filed: July 31, 2015
    Date of Patent: August 25, 2020
    Assignee: The General Hospital Corporation
    Inventors: Rebecca Sandlin, Keith Wong, Shannon Tessier, Mehmet Toner, Shannon Stott
  • Patent number: 10729703
    Abstract: The present invention provides gero-protective pharmaceutical compositions and methods, the compositions being adapted to enhance at least one of cell survival and cell metabolism in a mammalian subject, the pharmaceutical compositions include at least two of Withaferin a or its structural analogs, coumestrol, ginsenoside, quinidine, silymarin, licochalcone a, lipoic acid and apigenin, in a pharmaceutically effective amount.
    Type: Grant
    Filed: September 28, 2017
    Date of Patent: August 4, 2020
    Assignee: Insilico Medicine IP Limited
    Inventors: Aleksandrs Zavoronkovs, Alexander Aliper, Polina Mamoshina, Artem Artemov, Ivan Ozerov
  • Patent number: 10731132
    Abstract: The invention relates to method for the separation of cord blood endothelial cells from hematopoietic cells in a manner that preserves the hematopoietic cells' repopulating ability and primitivity, without significantly reducing the yield of hematopoietic cells. The said endothelial cells from cord blood have a utility in cell based therapeutics. The system described takes advantage of the endogenous adherence properties of the cells to facilitate the separation and separate storage of the cell types.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: August 4, 2020
    Assignee: Amniotics AB
    Inventors: Marcus Kare Torleif Larsson, Andreas Nils Walter Herbst, Niels-bjarne Roland Woods
  • Patent number: 10730020
    Abstract: A method for manufacturing a porous membrane includes: mixing silicon carbide powders and a coagulant to form a first mixture; adding a sintering aid to the first mixture to form a second mixture; compressing the second mixture; and sintering the compressed second mixture. More particularly, the coagulant is in an amount of 1% to 3% by weight of the silicon carbide powders and the sintering aid is in an amount of 10% by weight of the first mixture.
    Type: Grant
    Filed: April 20, 2018
    Date of Patent: August 4, 2020
    Inventors: Tung-Wen Cheng, Su-En Wu, Yi-Chun Lin, Chung-Kai Chang
  • Patent number: 10695693
    Abstract: In certain embodiments, the invention provides a method of purifying a protein of interest from a mixture which comprises the protein of interest and one or more contaminants, said method comprising: a) subjecting the mixture to a first chromatography matrix, wherein the protein of interest binds to the first chromatography matrix; b) contacting the first chromatography matrix with a first wash solution which has a pH of at least 9.0, and does not comprise arginine or an arginine derivative; and c) eluting the protein of interest from the first chromatography matrix into an elution solution.
    Type: Grant
    Filed: March 11, 2016
    Date of Patent: June 30, 2020
    Inventors: Jue Wang, Neil E. Jaffe, Krina Patel
  • Patent number: 10684283
    Abstract: This disclosure relates to the field of diagnostic methods. A new marker antibody for rheumatoid arthritis, as well as a new method for detecting rheumatoid arthritis, is disclosed herein. Also provided herein are means and methods for predicting whether a subject will develop rheumatoid arthritis. This disclosure is based on the discovery that patients with rheumatoid arthritis have antibodies in their circulation that react specifically with citrullinated antibodies. In one aspect, the disclosure, therefore, relates to an antibody comprising a citrulline residue. In another aspect, the disclosure provides a method for the detection of antibodies specific for rheumatoid arthritis in a sample from a subject, wherein the sample is contacted with a citrullinated antibody and wherein it is determined whether the sample comprises antibodies specifically reactive with the citrullinated antibody.
    Type: Grant
    Filed: April 22, 2015
    Date of Patent: June 16, 2020
    Assignee: NOVIO TH B.V.
    Inventor: Martinus Hubertus Leonardus Salden
  • Patent number: 10646511
    Abstract: This invention relates to medicine, more specifically, to cardiac surgery, and may be used for protecting the heart from ischemia when administering cardioplegia in normothermia or hypothermia. The general-purpose cardioplegic solution contains pharmaceutically acceptable potassium ions; magnesium ions; a base and an acid providing a pH buffer in a range of 7.1-8.9; a diuretic providing osmolality in a range of 275-460 mOsmol/kg. Said solution is used for cardiac protection in cardiopulmonary bypass, as well as for achieving asystole and maintaining the achieved asystole. The procedure for administering the general-purpose cardioplegic solution involves maintaining the achieved asystole by decreasing the flow rate of starting components of said solution relative to the flow rate of autoblood thus decreasing the solution to autoblood ratio.
    Type: Grant
    Filed: June 5, 2015
    Date of Patent: May 12, 2020
    Inventor: Valentin Evgenevich Volgushev
  • Patent number: 10626444
    Abstract: This disclosure provides methods, kits, and compositions to amplify sample DNA of low quantity by adding an amount of buffer DNA before amplification. The method protects the sample DNA against degradation and PCR bias. The method disclosed herein are useful to amplify DNA of low amount, such as genomic DNA from a single cell and therefore are especially useful for genetic diagnostic tests and precision medicine.
    Type: Grant
    Filed: October 11, 2017
    Date of Patent: April 21, 2020
    Assignee: Acudx Inc.
    Inventors: Xitong Li, Zhuanfen Cheng
  • Patent number: 10604751
    Abstract: Nucleic acids in non-rare cells in a sample containing non-rare cells and rare cells are selectively released from the non-rare cells. The sample is combined with an aqueous medium, and the combination is held for a period of time and at a temperature for selectively releasing nucleic acids from the non-rare cells but not from the rare cells. The sample is subjected to filtration to separate rare cells from non-rare cells. Rare cells with intact nucleic acids are separated from non-rare cells and nucleic acids from the non-rare cells. Nucleic acids from the rare cells are subjected to one or more identification techniques either with or without extraction of nucleic acids from the rare cells.
    Type: Grant
    Filed: July 2, 2015
    Date of Patent: March 31, 2020
    Assignee: Siemens Healthcare Disgnostics Inc.
    Inventors: Michael Pugia, Julia Philip, Karen Marfut
  • Patent number: 10591400
    Abstract: The present technology provides a technology for stabilizing break-off timings. Therefore, according to the present technology, there is provided a microparticle analysis device or the like including at least: a flow path in which a fluid including a sample flow containing microparticles and a sheath flow flowing to contain the sample flow; a droplet formation unit configured to form a droplet in the fluid by imparting vibration to the fluid using a vibration element; an electric charge application unit configured to apply electric charge to a droplet containing the microparticles; an imaging unit configured to obtain a photo of a phase of a certain time; and a control unit configured to control a timing at which the droplet breaks off on a basis of the photo.
    Type: Grant
    Filed: January 8, 2019
    Date of Patent: March 17, 2020
    Assignee: Sony Corporation
    Inventors: Yuya Suzuki, Fumitaka Otsuka, Masashi Kimoto, Shingo Imanishi, Yoshitsugu Sakai
  • Patent number: 10589191
    Abstract: Provided is a method for separating a substance accommodated in a vessel-shaped structure comprising subjecting a vessel-shaped structure 1, which includes a vessel portion a, a vessel portion b, and a self-fusing material X connecting the both vessel portions, and which accommodates a substance selected from the group consisting of a liquid, a solid a gas, and a dispersion system, to the steps: (i) pulling the vessel portion a and the vessel, portion b away from each other to extend the self-fusing material X; (ii) fusing the extended self-fusing material X together between the vessel portion a and the vessel portion b to separate the accommodated substance; and (iii) cutting a fused part of the self-fusing material X to separate the vessel-shaped structure 1 into a separated structure.
    Type: Grant
    Filed: May 31, 2012
    Date of Patent: March 17, 2020
    Inventors: Tetsuo Ohashi, Yukio Oikawa, Kei Shinada
  • Patent number: 10537097
    Abstract: Methods for storing or rejuvenating blood involve contacting blood with a blood storage composition or a blood rejuvenating composition that include D-ribose and a nucleoside other than inosine (e.g., guanosine).
    Type: Grant
    Filed: January 9, 2017
    Date of Patent: January 21, 2020
    Assignee: Viacell, LLC
    Inventor: Daniel G. Ericson
  • Patent number: 10525469
    Abstract: A hand-held molecular diagnostic test device includes a housing, an amplification (or PCR) module, and a detection module. The amplification module is configured to receive an input sample, and defines a reaction volume. The amplification module includes a heater such that the amplification module can perform a polymerase chain reaction (PCR) on the input sample. The detection module is configured to receive an output from the amplification module and a reagent formulated to produce a signal that indicates a presence of a target amplicon within the input sample. The amplification module and the detection module are integrated within the housing.
    Type: Grant
    Filed: April 5, 2019
    Date of Patent: January 7, 2020
    Assignee: Visby Medical, Inc.
    Inventors: Boris Andreyev, Keith E. Moravick, Brian Ciopyk, Victor Briones, Gregory Loney, Adam De La Zerda, Jesus Ching, Steven Chu, David Swenson, Helen Huang, Colin Kelly
  • Patent number: 10525186
    Abstract: A method of performing regional citrate anticoagulant dialysis of a patient's blood includes flowing blood from and back to the patient through an extracorporeal circuit including a dialyzer having semi-permeable dialysis membranes and a dialysate chamber surrounding the membranes. The method further includes flowing a dialysate containing calcium and citrate through the dialysate chamber of the dialyzer and introducing citrate into the patient's blood upstream of the dialyzer, whereby the patient's blood is dialyzed. The method can further include predicting the concentration of systemic ionized calcium in the blood of the patient at any point in the dialysis treatment or post-dialysis, such as by a mathematical model. The method can further include statistically correcting the preliminary predicted post-dialysis concentration of systemic ionized calcium in the patient's blood to provide a final predicted post-dialysis systemic ionized calcium concentration.
    Type: Grant
    Filed: August 19, 2014
    Date of Patent: January 7, 2020
    Assignee: Fresenius Medical Care Holdings, Inc.
    Inventors: Peter Kotanko, Stephan Thijssen, Nathan W. Levin