Maintaining Blood Or Sperm In A Physiologically Active State Or Compositions Thereof Or Therefor Or Methods Of In Vitro Blood Cell Separation Or Treatment Patents (Class 435/2)
  • Patent number: 11255841
    Abstract: Distributed liquid-flow systems—in which flow spreads out from a system inlet and traverses the system through multiple discrete, smaller flow channels—are constructed to minimize variations in flow-resistance-induced pressure drop from the system inlet to entrances to the flow channels. Because flow-driving pressure will be more uniform at the entrances to the flow channels, flow along the channels will be more uniform. Disclosed embodiments may be particularly suitable or advantageous for use in gas-exchange/artificial lung devices.
    Type: Grant
    Filed: August 1, 2019
    Date of Patent: February 22, 2022
    Assignee: Nano2cure Ltd.
    Inventor: Yoram Palti
  • Patent number: 11231425
    Abstract: An immunoassay includes forming first mixtures by reacting a combination reagent with serum of a subject; simultaneously forming second mixtures by reacting randomly selected platelet samples with the serum wherein in each mixture there are immunity compounds formed by combining the platelet antigens with predetermined antibodies in the serum, and other platelet antigens and other antibodies in the serum not forming the immunity compounds; preparing an interception device including receptacles and a filter net; placing each mixture in one receptacle; washing the mixtures wherein the mixtures forming the immunity compounds are intercepted by the filter net with others passing through; adding a signal sensing reagent to each receptacle; reacting the signal sensing reagent with the intercepted mixtures forming the immunity compounds to form final products; and performing a signal sensing to determine whether the final products contain anti-platelet antibodies and determine compatibility of cross matching of resp
    Type: Grant
    Filed: September 11, 2019
    Date of Patent: January 25, 2022
    Inventors: Tzong-Shi Chiueh, Min-Hsien Wu, Hsin-Yao Wang
  • Patent number: 11224217
    Abstract: Disclosed herein are methods and compositions for preserving avulsed teeth and/or explanted tissue. The disclosed methods for preserving avulsed teeth and/or explanted tissue typically include placing the avulsed teeth and/or explanted tissue in a preservation solution comprising at least about 2.75 g/L sugar. Suitable explanted tissues for the disclosed methods may include explanted tissues comprising fibroblasts, such as dental tissue and/or oral tissue. The preservation solution of the disclosed preservation methods may include additional components which may include but are not limited to inorganic salts, amino acids, vitamins, and buffering agents. The preservation solution may be prepared from a powder formulation having a long shelf-life.
    Type: Grant
    Filed: April 24, 2019
    Date of Patent: January 18, 2022
    Assignee: Marquette University
    Inventors: Lobat Tayebi, Morteza Rasoulianboroujeni
  • Patent number: 11197893
    Abstract: A pure platelet-rich plasma (P-PRP) composition as an alternative to conventional antibiotic treatment of subclinical mastitis caused by Gram-positive bacteria in bovine including five live platelets and leukocytes, an anticoagulant, and an activating substance.
    Type: Grant
    Filed: November 8, 2016
    Date of Patent: December 14, 2021
    Assignee: Universidad de Caldas
    Inventors: Alejandro Ceballos, Jorge U. Carmona
  • Patent number: 11191880
    Abstract: A system for processing fluids and filling a container with a product includes a disposable fluid circuit and reusable hardware configured to accept the disposable fluid circuit. The disposable fluid circuit includes a spinning membrane separator, first and second syringes, and a flow control cassette. The reusable hardware includes a drive coupled to the spinning membrane separator, first and second syringe pumps, the first and second syringes coupled to the first and second syringe pumps respectively, and a controller. The system also includes a syringe pump for filling low-volume containers, which syringe pump may be one of the first and second syringe pumps, or may be a third syringe pump. The syringe pump for filling low-volume containers may include a filtered vacuum/pressure source and a position detector.
    Type: Grant
    Filed: May 14, 2019
    Date of Patent: December 7, 2021
    Assignee: FENWAL, INC.
    Inventors: Kyungyoon Min, Christopher J. Wegener
  • Patent number: 11185068
    Abstract: Provided are a diluent and a sperm preservation method using the diluent. The diluent is useful in preservation of sperm with high fertility, and is capable of stably achieving quality control and improving quality of the sperm. Refrigerating or freezing sperm using the diluent for sperm, which includes an aqueous solution containing at least one oligosaccharide selected from the group consisting of a fructo-oligosaccharide, an isomalto-oligosaccharide, a gentio-oligosaccharide, and a galacto-oligosaccharide, can improve the quality of preserved sperm and provide sperm having high fertility, at a reduced cost.
    Type: Grant
    Filed: September 13, 2018
    Date of Patent: November 30, 2021
    Assignee: Livestock Improvement Association of Japan, Inc.
    Inventors: Masashi Kinukawa, Katsutoshi Funauchi, Kyoko Uchiyama
  • Patent number: 11166453
    Abstract: Provided herein is technology relating to cryopreservation and particularly, but not exclusively, to devices, systems, and methods for cryopreservation of biological materials such as oocytes, zygotes, and embryos. In particular, provided herein are methods of using microfluidic devices to exposing biological material to a vitrification solution having a time dependent concentration of a cryoprotectant agent.
    Type: Grant
    Filed: October 23, 2018
    Date of Patent: November 9, 2021
    Assignee: The Regents of the University of Michigan
    Inventors: David Lai, Shuichi Takayama, Gary D. Smith, Jun Ding
  • Patent number: 11090331
    Abstract: Method for transfusion medicine to reduce adverse events in transfusion patient populations based on underlying patient physiology. Methods for reducing the risk of an inflammatory response in a sickle cell patient in need of a blood transfusion.
    Type: Grant
    Filed: May 15, 2020
    Date of Patent: August 17, 2021
    Assignee: Hemanext Inc.
    Inventors: Angelo D'Alessandro, Rafael Cordero, Andrew Dunham, Philip Keegan, Tatsuro Yoshida
  • Patent number: 11077241
    Abstract: The present invention is related to the field of tissue regeneration. It concerns more particularly new standardizations and medical devices for the preparation of A-PRP, PRP, BMC, fat tissue, alone or in combination with a biomaterial or cell extract.
    Type: Grant
    Filed: November 26, 2015
    Date of Patent: August 3, 2021
    Assignee: REGEN LAB USA LLC
    Inventor: Antoine Turzi
  • Patent number: 11069813
    Abstract: A method of forming a semiconductor device includes forming source/drain contact openings extending through at least one dielectric layer to expose source/drain contact regions of source/drain structures. The method further includes forming conductive plugs in the source/drain contact openings. The method further includes depositing a light blocking layer over the conductive plugs and the at least one dielectric layer. The method further includes etching the light blocking layer to expose the conductive plugs. The method further includes directing a laser irradiation to the conductive plugs and the light blocking layer. The laser irradiation is configured to activate dopants in the source/drain contact regions.
    Type: Grant
    Filed: September 30, 2019
    Date of Patent: July 20, 2021
    Assignee: Taiwan Semiconductor Manufacturing Co., Ltd.
    Inventors: Blandine Duriez, Marcus Johannes Henricus Van Dal, Martin Christopher Holland, Gerben Doornbos, Georgios Vellianitis
  • Patent number: 11033900
    Abstract: A method for treating a blood specimen with which capturing rates for both a small rare cell and a rare cell having a high deformability can be improved in the case where rare cells are contained in a blood specimen. The method for isolating or detecting a rare cell includes treating a blood specimen using a filter to isolate or detect a rare cell in the blood specimen.
    Type: Grant
    Filed: March 23, 2016
    Date of Patent: June 15, 2021
    Assignee: ARKRAY, Inc.
    Inventor: Hidenori Takagi
  • Patent number: 11008566
    Abstract: The present invention relates to a method for obtaining an antibody from an avian B cell antibody library, comprising the following steps (a) to (d): (a) a step of allowing an avian B cell antibody library to come into contact with an antigen in the presence of a calcineurin inhibitor and avian serum, (b) a step of selecting avian B cells that bind to the antigen in the step (a), (c) a step of culturing the avian B cells selected in the step (b) in the presence of a calcineurin inhibitor and avian serum, and (d) a step of obtaining the avian B cells obtained through the step (c) and/or an antibody expressed by the avian B cells.
    Type: Grant
    Filed: September 29, 2017
    Date of Patent: May 18, 2021
    Assignee: Chiome Bioscience Inc.
    Inventors: Yosuke Kamimura, Kotaro Yamamoto
  • Patent number: 10996218
    Abstract: The present invention provides a method for determining the gender of a fetus by assaying the sex hormones, evaluating the overall reducing/oxidizing (redox) activity, and/or evaluating the radical scavenging capacity of the maternal urine or other body fluid. The method can be used to determine fetal gender at any time point during pregnancy. The methods of the present invention also provide for a means for pre-conception offspring gender planning by assaying the sex hormones in urine samples, evaluating the overall reducing/oxidizing (redox) activity, and/or evaluating the radical scavenging capacity of the urine or other body fluid from a non-pregnant female. In some embodiments, the methods described herein are used to sort sperm based on an overall reducing/oxidizing environment of follicular fluids, which can then be used for intrauterine insemination.
    Type: Grant
    Filed: June 14, 2018
    Date of Patent: May 4, 2021
    Inventor: Kuldeep C. Verma
  • Patent number: 10980460
    Abstract: The present invention relates to devices and methods of use thereof for detection of biomolecules, in vitro, in vivo, or in situ. The invention relates to methods of diagnosing and/or treating a subject as having or being at risk of developing a disease or condition that is associated with abnormal levels of one or more biomolecules including, but not limited to, inter alia, epilepsy, diseases of the basal ganglia, athetoid, dystonic diseases, neoplasms, Parkinson's disease, brain injuries, spinal cord injuries, and cancer. The invention also provides methods of differentiating white matter from gray matter. In some embodiments, regions of the brain to be resected or targeted for pharmaceutical therapy are identified using sensors. The invention further provides methods of measuring the neurotoxicity of a material by comparing microvoltammograms of a neural tissue in the presence and absence of the material using the inventive sensors.
    Type: Grant
    Filed: February 15, 2017
    Date of Patent: April 20, 2021
    Assignees: Research Foundation of The City University of New York, New York University School of Medicine
    Inventors: Patricia A. Broderick, Steven V. Pacia
  • Patent number: 10946046
    Abstract: A method for sterilising a platelet lysate in the liquid state comprising at least the endogenous growth factors TGF-beta 1, EGF, PDGF-AB, IGF-1, VEGF and bFGF. The method comprising freezing the liquid platelet lysate in order to obtain a frozen platelet lysate, and irradiating the frozen platelet lysate with ionising radiation in order to obtain a sterilised platelet lysate, the irradiation being adapted so as to preserve at least 80% of the concentration of at least one of the endogenous growth factors chosen from the group consisting of TGF-beta 1, EGF, PDGF-AB, IGF-1 and VEGF.
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: March 16, 2021
    Assignee: Maco Pharma
    Inventors: Bruno Delorme, Sabrina Viau, Francis Goudaliez
  • Patent number: 10940260
    Abstract: Provided is a blood component separation device and related method, each capable of separating a blood component from blood and rapidly performing a virus inactivation process on the separated blood component. The blood component separation device includes a blood component separation section, provided in a centrifuge, configured to separate a blood component from blood by centrifugation, a diluting section configured to dilute, in a diluent containing riboflavin, concentrated red blood cells separated by the blood component separation section, and a UV light emitting unit configured to perform a virus inactivation process by exposing the concentrated red blood cells thus diluted to the UV light.
    Type: Grant
    Filed: March 13, 2017
    Date of Patent: March 9, 2021
    Assignee: Terumo Kabushiki Kaisha
    Inventor: Masatsugu Igarashi
  • Patent number: 10935560
    Abstract: The invention described herein relates to a method and kit for the diagnosis of antiphospholipid syndrome in patients, wherein a coagulation inhibitor and normal pooled plasma mixture reduce the factor deficiency effect of patient plasma, and coagulation inhibitor interference in patient plasma, and increases specificity and the sensitivity for detecting lupus anticoagulants in the patient's blood in a phospholipid-dependent clotting assay.
    Type: Grant
    Filed: April 19, 2017
    Date of Patent: March 2, 2021
    Assignee: Instrumentation Laboratory Company
    Inventors: Ralph E. Bottenus, Zhenghua Cao
  • Patent number: 10888259
    Abstract: Disclosed herein are devices, apparatus, systems, methods and kits for collecting and storing a fluid sample from a subject. A device for collecting the fluid sample can include a housing comprising a recess having an opening, a vacuum chamber in the housing and in fluidic communication with the recess, and one or more piercing elements that are extendable through the opening to penetrate skin of the subject. The vacuum chamber can be configured for having a vacuum that draws the skin into the recess. The recess can be configured having a size or shape that enables an increased volume of the fluid sample to be accumulated in the skin drawn into the recess.
    Type: Grant
    Filed: November 15, 2019
    Date of Patent: January 12, 2021
    Assignee: Drawbridge Health, Inc.
    Inventors: Brett L. Jordan, Masao Drexel, Dagmar Beyerlein, Alicia Jackson, Kara Juneau
  • Patent number: 10850280
    Abstract: Methods, devices, and kits, related to embodiments for uniformly coating at least one inner surface of a capillary with at least one anticoagulant compound.
    Type: Grant
    Filed: November 14, 2018
    Date of Patent: December 1, 2020
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventor: Aaron Kauffmann
  • Patent number: 10815456
    Abstract: The present disclosure provides for a cryopreservation composition comprising a permeating cryoprotectant, a saccharide and a macromolecule. The cryopreservation composition has a low toxicity to cells and tissues, and promotes survival and retention of viability of the biological material during cryopreservation.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: October 27, 2020
    Inventors: Bin-Ru She, Kun-Chi Chiang, Cheng-Yi Lin, Ting-Yu Huang
  • Patent number: 10780438
    Abstract: An interfacial technique utilizes hydrodynamic micro-vortices to perform (i) high efficiency single cell encapsulation and (ii) size-selective capturing of cells based on their sizes in a single microfluidic device. A notable feature of this technique is that it can perform high efficiency single cell encapsulation at low cell concentrations, and this technique is all passive, controlled only by the flow rates of the two phases and does not require complex structures or on-chip active devices. Single bead/cell encapsulation was demonstrated at 50% efficiency, which is at least 10 times greater than the random encapsulations at the introduced cell concentrations. Also demonstrated is the selective trapping of cells based on their sizes. This present technique expands the capabilities of droplet microfluidics for applications ranging from single cell genomics, proteomic assays to sample preparation.
    Type: Grant
    Filed: June 11, 2018
    Date of Patent: September 22, 2020
    Inventors: Gopakumar Kamalakshakurup, Abraham P. Lee
  • Patent number: 10763387
    Abstract: A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.
    Type: Grant
    Filed: May 3, 2019
    Date of Patent: September 1, 2020
    Assignee: ABS Global, Inc.
    Inventors: Frederick Hershel Savage, Glenn J. Szejna, Zheng Xia
  • Patent number: 10750739
    Abstract: Methods for stabilizing blood samples, e.g., clinical blood samples, for storage or transportation before use. Exemplary applications include but are not limited to enrichment of leukocyte subtypes such as T-cells or neutrophils for cytokine and immuno-assays; isolation of progenitor cells from cord blood or peripheral blood for transplantation; isolation of fetal cells from the maternal blood for diagnosis; and sorting of circulating tumor cells for cancer detection and therapy.
    Type: Grant
    Filed: July 31, 2015
    Date of Patent: August 25, 2020
    Assignee: The General Hospital Corporation
    Inventors: Rebecca Sandlin, Keith Wong, Shannon Tessier, Mehmet Toner, Shannon Stott
  • Patent number: 10731132
    Abstract: The invention relates to method for the separation of cord blood endothelial cells from hematopoietic cells in a manner that preserves the hematopoietic cells' repopulating ability and primitivity, without significantly reducing the yield of hematopoietic cells. The said endothelial cells from cord blood have a utility in cell based therapeutics. The system described takes advantage of the endogenous adherence properties of the cells to facilitate the separation and separate storage of the cell types.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: August 4, 2020
    Assignee: Amniotics AB
    Inventors: Marcus Kare Torleif Larsson, Andreas Nils Walter Herbst, Niels-bjarne Roland Woods
  • Patent number: 10730020
    Abstract: A method for manufacturing a porous membrane includes: mixing silicon carbide powders and a coagulant to form a first mixture; adding a sintering aid to the first mixture to form a second mixture; compressing the second mixture; and sintering the compressed second mixture. More particularly, the coagulant is in an amount of 1% to 3% by weight of the silicon carbide powders and the sintering aid is in an amount of 10% by weight of the first mixture.
    Type: Grant
    Filed: April 20, 2018
    Date of Patent: August 4, 2020
    Inventors: Tung-Wen Cheng, Su-En Wu, Yi-Chun Lin, Chung-Kai Chang
  • Patent number: 10729703
    Abstract: The present invention provides gero-protective pharmaceutical compositions and methods, the compositions being adapted to enhance at least one of cell survival and cell metabolism in a mammalian subject, the pharmaceutical compositions include at least two of Withaferin a or its structural analogs, coumestrol, ginsenoside, quinidine, silymarin, licochalcone a, lipoic acid and apigenin, in a pharmaceutically effective amount.
    Type: Grant
    Filed: September 28, 2017
    Date of Patent: August 4, 2020
    Assignee: Insilico Medicine IP Limited
    Inventors: Aleksandrs Zavoronkovs, Alexander Aliper, Polina Mamoshina, Artem Artemov, Ivan Ozerov
  • Patent number: 10695693
    Abstract: In certain embodiments, the invention provides a method of purifying a protein of interest from a mixture which comprises the protein of interest and one or more contaminants, said method comprising: a) subjecting the mixture to a first chromatography matrix, wherein the protein of interest binds to the first chromatography matrix; b) contacting the first chromatography matrix with a first wash solution which has a pH of at least 9.0, and does not comprise arginine or an arginine derivative; and c) eluting the protein of interest from the first chromatography matrix into an elution solution.
    Type: Grant
    Filed: March 11, 2016
    Date of Patent: June 30, 2020
    Inventors: Jue Wang, Neil E. Jaffe, Krina Patel
  • Patent number: 10684283
    Abstract: This disclosure relates to the field of diagnostic methods. A new marker antibody for rheumatoid arthritis, as well as a new method for detecting rheumatoid arthritis, is disclosed herein. Also provided herein are means and methods for predicting whether a subject will develop rheumatoid arthritis. This disclosure is based on the discovery that patients with rheumatoid arthritis have antibodies in their circulation that react specifically with citrullinated antibodies. In one aspect, the disclosure, therefore, relates to an antibody comprising a citrulline residue. In another aspect, the disclosure provides a method for the detection of antibodies specific for rheumatoid arthritis in a sample from a subject, wherein the sample is contacted with a citrullinated antibody and wherein it is determined whether the sample comprises antibodies specifically reactive with the citrullinated antibody.
    Type: Grant
    Filed: April 22, 2015
    Date of Patent: June 16, 2020
    Assignee: NOVIO TH B.V.
    Inventor: Martinus Hubertus Leonardus Salden
  • Patent number: 10646511
    Abstract: This invention relates to medicine, more specifically, to cardiac surgery, and may be used for protecting the heart from ischemia when administering cardioplegia in normothermia or hypothermia. The general-purpose cardioplegic solution contains pharmaceutically acceptable potassium ions; magnesium ions; a base and an acid providing a pH buffer in a range of 7.1-8.9; a diuretic providing osmolality in a range of 275-460 mOsmol/kg. Said solution is used for cardiac protection in cardiopulmonary bypass, as well as for achieving asystole and maintaining the achieved asystole. The procedure for administering the general-purpose cardioplegic solution involves maintaining the achieved asystole by decreasing the flow rate of starting components of said solution relative to the flow rate of autoblood thus decreasing the solution to autoblood ratio.
    Type: Grant
    Filed: June 5, 2015
    Date of Patent: May 12, 2020
    Inventor: Valentin Evgenevich Volgushev
  • Patent number: 10626444
    Abstract: This disclosure provides methods, kits, and compositions to amplify sample DNA of low quantity by adding an amount of buffer DNA before amplification. The method protects the sample DNA against degradation and PCR bias. The method disclosed herein are useful to amplify DNA of low amount, such as genomic DNA from a single cell and therefore are especially useful for genetic diagnostic tests and precision medicine.
    Type: Grant
    Filed: October 11, 2017
    Date of Patent: April 21, 2020
    Assignee: Acudx Inc.
    Inventors: Xitong Li, Zhuanfen Cheng
  • Patent number: 10604751
    Abstract: Nucleic acids in non-rare cells in a sample containing non-rare cells and rare cells are selectively released from the non-rare cells. The sample is combined with an aqueous medium, and the combination is held for a period of time and at a temperature for selectively releasing nucleic acids from the non-rare cells but not from the rare cells. The sample is subjected to filtration to separate rare cells from non-rare cells. Rare cells with intact nucleic acids are separated from non-rare cells and nucleic acids from the non-rare cells. Nucleic acids from the rare cells are subjected to one or more identification techniques either with or without extraction of nucleic acids from the rare cells.
    Type: Grant
    Filed: July 2, 2015
    Date of Patent: March 31, 2020
    Assignee: Siemens Healthcare Disgnostics Inc.
    Inventors: Michael Pugia, Julia Philip, Karen Marfut
  • Patent number: 10591400
    Abstract: The present technology provides a technology for stabilizing break-off timings. Therefore, according to the present technology, there is provided a microparticle analysis device or the like including at least: a flow path in which a fluid including a sample flow containing microparticles and a sheath flow flowing to contain the sample flow; a droplet formation unit configured to form a droplet in the fluid by imparting vibration to the fluid using a vibration element; an electric charge application unit configured to apply electric charge to a droplet containing the microparticles; an imaging unit configured to obtain a photo of a phase of a certain time; and a control unit configured to control a timing at which the droplet breaks off on a basis of the photo.
    Type: Grant
    Filed: January 8, 2019
    Date of Patent: March 17, 2020
    Assignee: Sony Corporation
    Inventors: Yuya Suzuki, Fumitaka Otsuka, Masashi Kimoto, Shingo Imanishi, Yoshitsugu Sakai
  • Patent number: 10589191
    Abstract: Provided is a method for separating a substance accommodated in a vessel-shaped structure comprising subjecting a vessel-shaped structure 1, which includes a vessel portion a, a vessel portion b, and a self-fusing material X connecting the both vessel portions, and which accommodates a substance selected from the group consisting of a liquid, a solid a gas, and a dispersion system, to the steps: (i) pulling the vessel portion a and the vessel, portion b away from each other to extend the self-fusing material X; (ii) fusing the extended self-fusing material X together between the vessel portion a and the vessel portion b to separate the accommodated substance; and (iii) cutting a fused part of the self-fusing material X to separate the vessel-shaped structure 1 into a separated structure.
    Type: Grant
    Filed: May 31, 2012
    Date of Patent: March 17, 2020
    Inventors: Tetsuo Ohashi, Yukio Oikawa, Kei Shinada
  • Patent number: 10537097
    Abstract: Methods for storing or rejuvenating blood involve contacting blood with a blood storage composition or a blood rejuvenating composition that include D-ribose and a nucleoside other than inosine (e.g., guanosine).
    Type: Grant
    Filed: January 9, 2017
    Date of Patent: January 21, 2020
    Assignee: Viacell, LLC
    Inventor: Daniel G. Ericson
  • Patent number: 10525469
    Abstract: A hand-held molecular diagnostic test device includes a housing, an amplification (or PCR) module, and a detection module. The amplification module is configured to receive an input sample, and defines a reaction volume. The amplification module includes a heater such that the amplification module can perform a polymerase chain reaction (PCR) on the input sample. The detection module is configured to receive an output from the amplification module and a reagent formulated to produce a signal that indicates a presence of a target amplicon within the input sample. The amplification module and the detection module are integrated within the housing.
    Type: Grant
    Filed: April 5, 2019
    Date of Patent: January 7, 2020
    Assignee: Visby Medical, Inc.
    Inventors: Boris Andreyev, Keith E. Moravick, Brian Ciopyk, Victor Briones, Gregory Loney, Adam De La Zerda, Jesus Ching, Steven Chu, David Swenson, Helen Huang, Colin Kelly
  • Patent number: 10525186
    Abstract: A method of performing regional citrate anticoagulant dialysis of a patient's blood includes flowing blood from and back to the patient through an extracorporeal circuit including a dialyzer having semi-permeable dialysis membranes and a dialysate chamber surrounding the membranes. The method further includes flowing a dialysate containing calcium and citrate through the dialysate chamber of the dialyzer and introducing citrate into the patient's blood upstream of the dialyzer, whereby the patient's blood is dialyzed. The method can further include predicting the concentration of systemic ionized calcium in the blood of the patient at any point in the dialysis treatment or post-dialysis, such as by a mathematical model. The method can further include statistically correcting the preliminary predicted post-dialysis concentration of systemic ionized calcium in the patient's blood to provide a final predicted post-dialysis systemic ionized calcium concentration.
    Type: Grant
    Filed: August 19, 2014
    Date of Patent: January 7, 2020
    Assignee: Fresenius Medical Care Holdings, Inc.
    Inventors: Peter Kotanko, Stephan Thijssen, Nathan W. Levin
  • Patent number: 10527607
    Abstract: This disclosure provides a method for adjusting the maturation state of mammalian sperm for use in assisted reproductive technologies (ART). A mammalian ejaculate is provided and incubated under controlled conditions. Aliquots of the ejaculate are assayed during incubation period at intervals to determine maturation state and changes in the maturation state by observing the percent positive cells in the aliquot. The assays are repeated with successive aliquots at intervals during incubation to observe real time changes in the maturation state. The ejaculate remaining is processed for the desired ART after the percentage of positive cells in the latest aliquot being assayed begins to decline.
    Type: Grant
    Filed: March 11, 2014
    Date of Patent: January 7, 2020
    Inventor: Barb A. Cohen
  • Patent number: 10509028
    Abstract: The present disclosure relates to a device for detecting an analyte in a sample, and especially for detecting antigen/antibody reactions, wherein said device comprises a layer composed of a low-density immiscible compound. The disclosure also relates to a method for detecting an analyte in a sample and especially for detecting antigen/antibody reactions in a device comprising a layer composed of a low-density immiscible compound. The disclosure further relates to the uses of such low-density immiscible compound.
    Type: Grant
    Filed: May 18, 2016
    Date of Patent: December 17, 2019
    Inventors: Julien Agusti, Stephane Bombard, Frederic Buffiere
  • Patent number: 10470457
    Abstract: Disclosed herein are methods and compositions for the cryopreservation of stem cells, such as stem-cell derived retinal pigment epithelial cells, that have been seeded onto and cultured on a substrate, such as a polymeric substrate. Such cryopreserved stem cells are useful for cell therapies, such as treatment of ocular damage or disease.
    Type: Grant
    Filed: January 27, 2017
    Date of Patent: November 12, 2019
    Assignee: University of Southern California
    Inventors: Danhong Zhu, David Hinton, Ashish Ahuja, Mark Humayun
  • Patent number: 10465167
    Abstract: An adjuvant for rapid proliferation of human mesenchymal stem cells in vitro is provided to overcome the problem of low cell amplification efficiency of human mesenchymal stem cells in a culture process. The adjuvant added for the culture of human mesenchymal stem cells includes at least one antioxidant, and a basic fibroblast growth factor (FGF-2). The adjuvant is added into a medium containing the human mesenchymal stem cells, and the culture takes place in a normal oxygen environment (21% oxygen tension), and the cells are divided rapidly, and the cell cycle at synthesis phase (S phase) percentage is increased to reduce ageing and improve differentiation potential. The adjuvant not only amplifies human mesenchymal stem cells rapidly to harvest the growth factor, but also maintains the characteristics of the multifunction of stem cells for the purposes of culturing and amplifying the human mesenchymal stem cells.
    Type: Grant
    Filed: December 11, 2013
    Date of Patent: November 5, 2019
    Assignee: Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation
    Inventors: Li-Yi Sun, Cheng-Yoong Pang, Ching-Feng Cheng, Dian-Kun Li
  • Patent number: 10444124
    Abstract: A clearing reagent according to the present invention for making a biological material transparent is a solution containing: at a concentration of 1M or more and not more than 8.5M, at least one compound selected from the group consisting of urea and urea derivatives; and glycerol at a concentration of 25 (w/v) % or more and not more than 35 (w/v) %.
    Type: Grant
    Filed: May 18, 2012
    Date of Patent: October 15, 2019
    Assignee: RIKEN
    Inventors: Atsushi Miyawaki, Hiroshi Hama, Asako Sakaue
  • Patent number: 10436686
    Abstract: The invention relates to a method of separating biological particles from the liquid containing same for purification, analysis and optionally diagnostic purposes. The inventive method comprises at least one step involving vertical filtration through a filter having a porosity that is adapted to the type of biological particles to be separated, such that said particles are retained by the filter. The invention is characterised in that: (i) the method involves the use of a filter comprising at least one basic filtration zone, whereby each basic filtration zone has a limited surface area; and (ii) the surface area of each basic filtration zone and the number of basic filtration zones are selected as a function of the type of liquid to be filtered, the type of biological particles to be separated and the volume of liquid to be filtered.
    Type: Grant
    Filed: September 18, 2017
    Date of Patent: October 8, 2019
    Inventor: Patrizia Paterlini-Brechot
  • Patent number: 10385317
    Abstract: The disclosure provides methods of making a red blood cell, plasma, and platelet products having a uniform dose and volume. The method comprises pooling a plurality of blood units, leukoreducing the blood and inactivating any pathogen contained therein. Plasma, RBCs, and platelets are then divided into uniform dose and volume units which have an extended shelf life.
    Type: Grant
    Filed: May 30, 2012
    Date of Patent: August 20, 2019
    Inventors: Beth Shaz, Christopher D. Hillyer
  • Patent number: 10379026
    Abstract: The present invention relates to compositions comprising magnetic particles, the methods of using these compositions in processing animal sperm, the resulting sperm and embryo products, and the methods of use of these compositions to increase the efficiency, efficacy and/or speed of cell processing and artificial insemination techniques.
    Type: Grant
    Filed: December 4, 2015
    Date of Patent: August 13, 2019
    Assignee: Inguran, LLC
    Inventor: Kristie Krug
  • Patent number: 10376878
    Abstract: Method for the treatment of a biological sample comprising at least one cell and a liquid component; according to the method, a force is applied to the sample inserted in an inner chamber of a hollow device towards a filter which has pores with diameters from 2 nm to 1 ?m, so that at least part of the liquid component passes through the filter and the cell remains in the inner chamber, thus obtaining a concentrated sample; the filter has a surface facing the inner chamber of less than 12.6 mm2.
    Type: Grant
    Filed: December 28, 2012
    Date of Patent: August 13, 2019
    Assignee: Menarini Silicon Biosystems S.p.A.
    Inventors: Alex Calanca, Gianni Medoro, Nicolò Manaresi, Giuseppe Giorgini
  • Patent number: 10371622
    Abstract: This disclosure relates to a device in the form of a microfluidic chip. In particular, various features are incorporated into the microfluidic chip for aligning and orienting sperm in flow channels, as well as for separating selected subpopulations of sperm.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: August 6, 2019
    Assignee: Inguran, LLC
    Inventors: Johnathan Charles Sharpe, Nemanya Sedoglavich, Erin Koksal, Kris Buchanan, Blair Morad
  • Patent number: 10371702
    Abstract: Disclosed herein are methods of treatment of autoimmune diseases such as systemic lupus erythematosus (SLE) as well as clinical assays for detection of autoimmune disease activity in patients utilizing a PD1 ligand.
    Type: Grant
    Filed: October 5, 2015
    Date of Patent: August 6, 2019
    Inventors: Neelufar Mozaffarian, Anne M. Stevens
  • Patent number: 10357512
    Abstract: The present invention relates to an universal plasma, originating from a mixture of plasmas originating from donor individuals of blood groups A, B, AB and/or O and compatible with all the blood groups and the preparation process thereof.
    Type: Grant
    Filed: October 9, 2015
    Date of Patent: July 23, 2019
    Inventor: Abdessatar Chtourou
  • Patent number: 10330668
    Abstract: Compositions and methods determines post-transfusion survival of platelets and the suitability of platelet units for transfusion by measuring the levels of one or more markers in a platelet sample. A method determines post-transfusion survival of platelets (PL T) prior to transfusion, the method comprising the steps of: a) measuring the levels of one or more markers in a PL T sample selected from the group consisting of adenine, 13-HODE/9-HODE, Caprylate, Laurate, C-glycosyltryptophan, andro steroid monsulfate 2, and Unelucidated Compounds (UC) 1-4; b) comparing the level of the one or more markers in the PLT sample with the level of the one or more markers present in a control sample, wherein a higher or lower level of the one or more markers in the PL T sample is indicative of post-transfusion survival of platelets.
    Type: Grant
    Filed: July 21, 2014
    Date of Patent: June 25, 2019
    Assignee: Bloodworks
    Inventors: James Charles Zimring, Sherrill J. Slichter
  • Patent number: 10293098
    Abstract: A system for removing undesirable elements from blood. The system includes a centrifuge bowl to separate the blood into components according to relative densities of the components, a pump to provide wash solution that washes the blood in the centrifuge bowl, and a controller to wash the blood in the centrifuge bowl in a first wash and remove first undesirable elements and to wash the blood in the centrifuge bowl in a second wash and remove trapped undesirable elements. The controller to further mix the blood and the wash solution in the centrifuge bowl and provide diluted blood, separate the diluted blood into concentrated blood and the wash solution, fill the centrifuge bowl with previously concentrated blood to build a buffy coat, and empty the centrifuge bowl of the concentrated blood and the previously concentrated blood after the buffy coat is reached.
    Type: Grant
    Filed: September 11, 2018
    Date of Patent: May 21, 2019
    Assignee: Sorin Group Italia S.r.l.
    Inventors: Elisa Maculan, Laura Scholze