Maintaining Blood Or Sperm In A Physiologically Active State Or Compositions Thereof Or Therefor Or Methods Of In Vitro Blood Cell Separation Or Treatment Patents (Class 435/2)
  • Patent number: 10293098
    Abstract: A system for removing undesirable elements from blood. The system includes a centrifuge bowl to separate the blood into components according to relative densities of the components, a pump to provide wash solution that washes the blood in the centrifuge bowl, and a controller to wash the blood in the centrifuge bowl in a first wash and remove first undesirable elements and to wash the blood in the centrifuge bowl in a second wash and remove trapped undesirable elements. The controller to further mix the blood and the wash solution in the centrifuge bowl and provide diluted blood, separate the diluted blood into concentrated blood and the wash solution, fill the centrifuge bowl with previously concentrated blood to build a buffy coat, and empty the centrifuge bowl of the concentrated blood and the previously concentrated blood after the buffy coat is reached.
    Type: Grant
    Filed: September 11, 2018
    Date of Patent: May 21, 2019
    Assignee: Sorin Group Italia S.r.l.
    Inventors: Elisa Maculan, Laura Scholze
  • Patent number: 10271540
    Abstract: A medical fluid for a harvested organ, tissue or part thereof, for evaluation and/or preservation. The fluid includes cocaine or a stimulating analog thereof; noradrenaline; and/or adrenaline. In addition, the fluid includes an oncotic agent, such as dextran; hormones, such as thyroxin; triiodotyronine; cortisone, insulin; and electrolytes and optionally nutrients in substantially physiological concentrations in a physiologically acceptable medium. In addition, the medical fluid further includes albumin in a concentration not exceeding 5.0%, and an oxygen carrier, such as erythrocytes. Further components may be dopamine; hydrocortisone; methylprednisolone; and a vasopressor agent, such as desmopressin. The cocaine; adrenaline; and noradrenaline are present in concentrations of each about 0.010 ?M to 0.100 ?M, for example in a ratio of 1:1:1.
    Type: Grant
    Filed: August 5, 2016
    Date of Patent: April 30, 2019
    Inventor: Stig Steen
  • Patent number: 10271539
    Abstract: The invention provides an assay-ready frozen eukaryotic cell (stored frozen) in a composition that includes a cryopreservative and an inhibitor of xanthine oxidase, a kit containing a plurality of the assay-ready frozen eukaryotic cell, and a method of preparation thereof to minimize the variability in the performance of the cell, particularly in terms of undesirable intra- and inter-assay variability.
    Type: Grant
    Filed: November 21, 2014
    Date of Patent: April 30, 2019
    Inventors: Michael G. Tovey, Christophe Lallemand
  • Patent number: 10261080
    Abstract: An optical engine its use in a bench top flow cytometer, the optical engine having a set of lasers, each focused horizontally along an x-axis to a same horizontal position and vertically along a y-axis to a different vertical position along a same excitation plane of a flow cell, a set of optics that separate fluorescence of a same wavelength range into different locations in a focal plane of collection optics according to the different lasers by which the fluorescent light is excited; and a detector that selectively detects light from the different locations thereby distinguishing between fluorescence emitted within the same wavelength range as excited by the different lasers.
    Type: Grant
    Filed: July 15, 2017
    Date of Patent: April 16, 2019
    Assignee: ACEA Biosciences, Inc.
    Inventors: Nan Li, Lingbo Kong, Jian Wu, Ting Cheng, Yangde Qin, Xiaobo Wang
  • Patent number: 10247739
    Abstract: The present invention relates to a method for the immunological measurement using a hapten and an antibody binding to the hapten as a reference antibody, and a device for the immunological measurement using the reference antibody. According to the present invention, the method and the device for the immunological measurement can be used in an immunoassay by reflecting surrounding environments, for example, by reflecting a numerical value in a test zone, which is changed by external factors such as temperatures of a sample and a device, an amount and components of an injected sample, variations of the device, and the like, in a reference zone and changing the numerical value in the test zone, and can be useful in minimizing analysis errors due to a low probability of causing a non-specific reaction with a material in the sample.
    Type: Grant
    Filed: October 31, 2014
    Date of Patent: April 2, 2019
    Assignee: SK TELECOM CO., LTD.
    Inventors: Sunyoung Park, Jeongryul Kim, Seong Woo Kim, Kum-Joo Shin, Ji-Chul Lee
  • Patent number: 10226041
    Abstract: The present invention generally relates to compositions and methods for the handling of processed sperm populations including samples that are freshly collected, those transported as fresh samples, as well as samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the sperm cell. Such trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the sperm's ability to fertilize an egg, grow into a health embryo and produce a healthy offspring.
    Type: Grant
    Filed: August 17, 2017
    Date of Patent: March 12, 2019
    Assignee: Inguran, LLC
    Inventors: Richard Lenz, Juan Moreno, Ramakrishnan Vishwanath
  • Patent number: 10216144
    Abstract: The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.
    Type: Grant
    Filed: April 15, 2016
    Date of Patent: February 26, 2019
    Assignee: Premium Genetics (UK) LTD
    Inventors: Daniel Mueth, Joseph Plewa, Jessica Shireman, Amy Anderson, Lewis Gruber, Neil Rosenbaum
  • Patent number: 10196631
    Abstract: The invention is directed to a releasable conjugate comprising a biotinylated ligand having a biotin moiety, a ligand moiety (Ligand1) and a biotin-binding molecule (bbm) bound to the biotin moiety of the biotinylated ligand. The ligand moiety of the biotinylated ligand may be separated by a spacer group consisting of polyethylene glycol. Furthermore, the invention relates to a method for cleaving the releasable conjugate by providing biotin or streptavidin and an auxiliary release agent in a sufficient concentration to displace the biotin-binding molecule (bbm) from the biotin moiety of the biotinylated ligand and a method for separation of target cells from a cell sample utilizing the conjugate.
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: February 5, 2019
    Assignee: Miltenyi Biotec GmbH
    Inventors: Jennifer Brieden, Christian Dose
  • Patent number: 10188631
    Abstract: The present invention relates to a novel melatonin-based formulation, particularly a topical liquid composition of melatonin (or analog thereof) suitable for use in intrauterine washing performed during medically assisted reproduction (e.g. in vitro fertilization—IVF). Melatonin is notoriously unstable, especially in solution. The compositions of the invention exhibit high stability, which allows them to be kept for prolonged periods before their eventual use in the inhibition or prevention of embryonic implantation failure.
    Type: Grant
    Filed: February 15, 2016
    Date of Patent: January 29, 2019
    Assignee: ARES TRADING S.A.
    Inventor: Rita Agostinetto
  • Patent number: 10190091
    Abstract: The present invention relates to the use of a combination of: (i)a macromolecular erythrocyte sedimentation enhancer, and (ii) dimethyl sulphoxide (DMSO), dimethylglycine (DMG) and/or valine; to recover non-erythrocyte blood cells from a blood cell-containing sample and/or to prime non-erythrocyte blood cells to protect their integrity in subsequent cryopreservation step(s).
    Type: Grant
    Filed: April 15, 2014
    Date of Patent: January 29, 2019
    Assignee: CELLS 4 LIFE GROUP LLP
    Inventor: Jeffrey Drew
  • Patent number: 10183291
    Abstract: The invention relates to a method and system for generating droplets of an aqueous solution on a microfluidic chip with an air continuous phase. Specifically, the droplet generator according to the present invention is integrated into a microfluidic chip to generate and introduce droplets of an aqueous solution into the microfluidic chip. The droplets travelling in a network of chip channels may be captured in on-chip traps in a manner defined by hydrodynamic resistances of chip channels. A biological reaction may be performed on a droplet trapped on the microfluidic chip.
    Type: Grant
    Filed: December 29, 2017
    Date of Patent: January 22, 2019
    Assignee: University of Maryland, College Park
    Inventors: Ian M. White, Srinivasa Raghavan, Kunal R. Pandit
  • Patent number: 10166188
    Abstract: The present invention relates generally to the fields of immunology and vaccine technology. More specifically, the present invention relates to methods for vitrifying biological preparations, including peptides, antigens, antibodies, cells, and the like.
    Type: Grant
    Filed: May 31, 2012
    Date of Patent: January 1, 2019
    Assignee: MERIAL, INC.
    Inventor: Noel Yves Henri Jean Genin
  • Patent number: 10167310
    Abstract: The present invention relates to a system and method for producing high levels of autologous IL-1RA cytokine, comprising: a blood collection vessel (1), a cover (4), a portion of separation gel (2), an anticoagulant portion (3), a plasma collection syringe comprising a sharp needle (9), a buffy coat collection syringe (11) and an incubation tube with cover (15). The blood collection vessel is adapted such that, when containing the whole blood (5), and centrifuged after treatment, yields separation fractions comprising, a first fraction of RBCs sediment (6), a second fraction of said gel (2), a third fraction comprising WBCs, platelets and a fourth fraction of plasma solution (8).
    Type: Grant
    Filed: January 29, 2013
    Date of Patent: January 1, 2019
    Inventor: Aaron Esteron
  • Patent number: 10160952
    Abstract: To provide a method in which mononuclear cells are differentiated into a good balance of NK cells, NKT cells, and T cells, and said cells are made to proliferate. The present invention provides a method for manufacturing an immune cell-containing composition, said method including the following steps: a first step in which mononuclear cells are cultured in a medium containing anti-CD16 monoclonal antibodies and either IL-2 and/or IL-15; and a second step, after the first step, in which culturing is performed under conditions that make the mononuclear cells preferentially differentiate into cytotoxic T cells. This method may also include a third step, after the second step, in which culturing is performed in a medium containing anti-CD16 monoclonal antibodies and either IL-2 and/or IL-15.
    Type: Grant
    Filed: January 14, 2014
    Date of Patent: December 25, 2018
    Inventors: Hiroyuki Abe, Seiichi Yusa
  • Patent number: 10154664
    Abstract: The present invention is directed to systems and methods of processing aspirated adipose tissue for the isolation of stromal vascular fraction derived stem cells.
    Type: Grant
    Filed: October 5, 2012
    Date of Patent: December 18, 2018
    Inventor: David K Moscatello
  • Patent number: 10137154
    Abstract: The invention generally features compositions and methods for expanding long term hematopoietic stem cells (HSCs) in a population of cells. In particular, the invention relates to a method of expanding long term HSCs by culturing an initial population of HSCs with macrophages that promote self-renewal of long term HSCs. The expanded cell population provides a source of cells for therapeutic treatments utilizing HSC transplantation.
    Type: Grant
    Filed: July 14, 2014
    Date of Patent: November 27, 2018
    Assignee: BioVentures, LLC
    Inventors: Daohong Zhou, Yi Luo, Lijian Shao, Wei Feng, Jianhui Chang
  • Patent number: 10126315
    Abstract: The method is provided for characterizing agglomeration of particles in a liquid containing an analyte, including introducing liquid into a fluid chamber; mixing the liquid with a bifunctional reagent, lighting the fluid chamber using an excitation light beam extending through the fluid chamber in a longitudinal direction (X); acquiring at least one image using a matrix photodetector, each image including pixels (In(x,y), x,y representing the coordinates of a pixel of an image, the image (I(x,y)) being formed by radiation transmitted by the lighted fluid chamber; and calculating from at least one acquired image (I(x,y)), at least one indicator (Ind2) characterizing the particle agglomeration. The photodetector can be positioned less than 1 cm from the fluid chamber, and during the calculation step, the calculated indicator (Ind2) is representative of the intensity of the pixels of the image. The method advantageously allows for characterizing the agglomeration of particles in a liquid.
    Type: Grant
    Filed: April 8, 2016
    Date of Patent: November 13, 2018
    Inventors: Vincent Poher, Myriam-Laure Cubizolles, Patrick Pouteau, Cédric Allier, Johanna Spiaczka
  • Patent number: 10104880
    Abstract: Provided herein are improved methods for the formulation of compositions comprising placental stem cells, and improved compositions and cell formulations produced thereby.
    Type: Grant
    Filed: August 20, 2009
    Date of Patent: October 23, 2018
    Assignee: CELULARITY, INC.
    Inventors: Andy Zeitlin, Gregory Russotti, Shuyang He, Ajai Pal, Hong J. Chen, Thomas Brieva, Ryan Shorr, Brian Murphy
  • Patent number: 10088751
    Abstract: The present disclosure discloses a method of fabrication of free standing open pore membranes with uniform pore size and shape and ordered pore distribution, and its use for synthesis of nanoparticle patterns. The method includes applying a photoresist layer to the top surface of a substrate, heating the photoresist layer for a period of time, and exposing the photoresist layer to a dose of ultraviolet radiation through a mask having a predetermined pattern. The dose of ultraviolet radiation is controlled in intensity and time and the photoresist layer is exposed such that a top portion of the photoresist layer through which the dose of ultraviolet radiation enters the photoresist layer undergoes greater cross linking than a bottom portion of the photoresist layer immediately adjacent to the top surface of the substrate such that a cross linking gradient develops through a thickness of the photoresist layer.
    Type: Grant
    Filed: November 27, 2017
    Date of Patent: October 2, 2018
    Inventors: Jun Yang, Tingjie Li
  • Patent number: 10081678
    Abstract: This disclosure relates to specific binding agents, such as isolated recombinant monoclonal antibodies, that bind Glycoprotein Ib alpha (GPIba). More specifically, the disclosure relates to methods of preventing platelet ectodomain shedding, preventing platelet clearance and degradation, maintaining and increasing platelet blood serum levels for in vitro or in vivo applications. In some embodiments, the disclosure relates to the production, diagnostic use, and therapeutic use of monoclonal and polyclonal antibodies, and the antigen-binding fragments thereof, which specifically bind GPIba. Aspects of the disclosure also relate to hybridomas or other cell lines expressing such antibodies for specific binding agent. Compositions and methods for inhibiting shedding, clearance, or degradation, or treating diseases associated with GPIba ectodomain shedding are also described.
    Type: Grant
    Filed: February 3, 2014
    Date of Patent: September 25, 2018
    Assignees: Emory University, Children's Healthcare of Atlanta, Inc.
    Inventor: Renhao Li
  • Patent number: 10070889
    Abstract: The present invention provides a new and improved sperm stimulating additive comprising a certain amount of inorganic pyrophosphate (PPi). Addition of PPi in the media for human/animal in vitro fertilization (IVF) improves fertilization rate; addition of PPi in the semen extender for farm animal artificial insemination (AI) may improve pregnancy rates; furthermore, mammalian oocytes matured in vitro in a medium including PPi attain improved fertilization and developmental potential, while embryos cultured in medium supplemented with PPi have improved development to blastocyst.
    Type: Grant
    Filed: May 29, 2014
    Date of Patent: September 11, 2018
    Assignee: The Curators of the University of Missouri
    Inventors: Peter Sutovsky, Young-Joo Yi
  • Patent number: 10052349
    Abstract: The present invention relates to new plasma or new platelet-rich plasma preparations, new cell dissociation methods, new cell associations or compositions, a method of preparation thereof, a use thereof, devices for the preparation thereof and preparations containing such a platelet-rich plasma preparation and cell associations or compositions. Specifically, the invention provides plasma or platelet-rich plasma alone or in cell composition preparations for use in tissue regeneration and bone regeneration and pain reduction.
    Type: Grant
    Filed: May 25, 2017
    Date of Patent: August 21, 2018
    Assignee: RegenLab USA LLC
    Inventors: Antoine Turzi, Donald Francois Du Toit
  • Patent number: 10036006
    Abstract: A system is disclosed for washing a selected component and removing the selected component from a wash material. The selected component may include red blood cells that are washed to remove a rejuvenating solution. The red blood cells may then be removed with various systems, such as a standing acoustic wave system from the wash solution. Pumps and flow restrictors that provide steady flow from pumps that generate pulsed flow are also disclosed.
    Type: Grant
    Filed: October 21, 2014
    Date of Patent: July 31, 2018
    Assignee: Biomet Biologics, LLC
    Inventor: Michael D. Leach
  • Patent number: 9977021
    Abstract: The invention provides methods of isolating CD127lo/? immunosuppressive regulatory T cells which can be greatly enriched for FoxP3, methods of expanding the isolated cells, pharmaceutical compositions of such cells, and methods of their use in the treatment of autoimmune and other immune system mediated disorders.
    Type: Grant
    Filed: March 24, 2015
    Date of Patent: May 22, 2018
    Assignee: The Regents of the University of California
    Inventors: Jeffrey A. Bluestone, Weihong Liu, Amy Putnam
  • Patent number: 9968663
    Abstract: Use of erythrocytes containing arginine deirainase for the preparation of a medicinal product for lowering the plasma concentration of arginine in vivo. The use relates in particular to the treatment of arginine-dependent tumors, such as hepatocarcinoma and malignant melanoma, or inhibition of the synthesis of nitric oxide, and the prevention and/or treatment of septic shock.
    Type: Grant
    Filed: July 31, 2015
    Date of Patent: May 15, 2018
    Assignee: ERYTECH PHARMA
    Inventors: Yann Godfrin, Pierre-Olivier Goineau
  • Patent number: 9970045
    Abstract: Herein is provided a simple, reliable and accurate method for cellular analysis on hematology analyzers. In various aspects, the methods provide separation and/or differentiation between red blood cells (RBCs) and white blood cells (WBCs) by utilizing a fluorescent dye to selectively stain WBCs such that they emit stronger fluorescence signals. The method provides optimal detection limits on WBCs and RBCs, thereby allowing analysis of samples with sparse cellular concentrations. As few as one reagent may be used to prepare a single dilution for body fluid analysis, in order to simplify the body fluid analysis. Minimal damage to WBCs is attained using the lysis-free approach described in aspects of the disclosure.
    Type: Grant
    Filed: December 19, 2012
    Date of Patent: May 15, 2018
    Assignee: Abbott Laboratories
    Inventors: Jiong Wu, Emily H. Lin, Jihping Yang
  • Patent number: 9931357
    Abstract: Disclosed herein are compositions and methods for preventing or treating acute or chronic oral mucositis, esophagitis, enteritis, colitis, or gastrointestinal acute radiation syndrome (GI-ARS) caused by radiation exposure, using one or more enteron sorbent polymers administered gastrointestinally (e.g. orally, via feeding or gastric tube, via ostomy, or rectally).
    Type: Grant
    Filed: October 2, 2015
    Date of Patent: April 3, 2018
    Assignee: CytoSorbents Corporation
    Inventors: Humayra Begum Ali, Thomas D. Golobish, Vincent J. Capponi, Phillip P. Chan, Wei-Tai Young, Andrew Robert Scheirer
  • Patent number: 9911572
    Abstract: A method for controlling an interaction between droplet targets and a high power and high-repetition-rate laser beam at a laser focus position of the laser beam including providing a droplet generator for generating a train of droplets as a droplet target with a predetermined droplet frequency and velocity in a predetermined direction; providing a high power, high-repetition-rate laser for emitting a pulsed laser beam, which is focused in the laser focus position; aligning the droplet generator such that the train of droplets runs through the laser focus position; generating a train of droplets as a droplet target; and emitting a pulsed laser beam in synchronization with the train of droplets, such that the droplet target interacts with the pulsed laser beam at the laser focus position.
    Type: Grant
    Filed: July 5, 2013
    Date of Patent: March 6, 2018
    Assignee: ETH Zurich
    Inventors: Nadia Gambino, Reza Abhari, Flori Alickaj, Felix Daners, Andrea Giovannini, Oran Morris, Bob Rollinger
  • Patent number: 9867782
    Abstract: The present invention is directed to a method of preparing dehydrated blood products, comprising the steps of: (a) providing a hydrated blood product; (b) spray-drying the hydrated blood product to produce a dehydrated blood product, as well as dehydrated blood products made by the method. The present invention is directed to a method of treating a patient suffering from a blood-related disorder, comprising the steps of: (a) rehydrating a therapeutic amount of the dehydrated blood products to produce a rehydrated therapeutic composition; and (b) administering the rehydrated therapeutic composition to the patient. The present invention is directed to a bandage or surgical aid comprising the dehydrated blood products described above.
    Type: Grant
    Filed: April 6, 2010
    Date of Patent: January 16, 2018
    Assignee: ENTEGRION, INC.
    Inventors: Thomas H. Fischer, Joseph A. DaCorta, Michael Lawrence Galiger
  • Patent number: 9845488
    Abstract: A method for isolating biomolecules from a fixed biological specimen embedded in paraffin, comprises the steps of bringing the specimen into contact a) with a solvent non-miscible with water and dissolving the paraffin in this solvent such as to form a liquid organic phase, and b) with an aqueous solution of at least one lysis substance and/or with water and at least one lysis substance such as to form an aqueous phase; isolation of the biomolecules from the aqueous phase. The invention further relates to a corresponding kit as well as to the use of such a kit for isolating biomolecules.
    Type: Grant
    Filed: June 30, 2010
    Date of Patent: December 19, 2017
    Assignee: Axagarius GmbH & Co. KG
    Inventors: Christoph Kirsch, Claudia Beyard, Markus Meusel, Klaus Moeller
  • Patent number: 9835541
    Abstract: In flow cytometry, particles (2) can be distinguished between populations (8) by combining n-dimensional parameter data, which may be derived from signal data from a particle, to mathematically achieve numerical results representative of an alteration (48). An alteration may include a rotational alteration, a scaled alteration, or perhaps even a translational alteration. Alterations may enhance separation of data points which may provide real-time classification (49) of signal data corresponding to individual particles into one of at least two populations.
    Type: Grant
    Filed: March 14, 2017
    Date of Patent: December 5, 2017
    Assignee: Beckman Coulter, Inc.
    Inventors: George C. Malachowski, Paul Barclay Purcell, Edward Allan Stanton, Kenneth Michael Evans
  • Patent number: 9795659
    Abstract: This invention discloses the process for lyophilization of the treated cells which comprises the use of a solution containing Trehalose along with amphipathic polymer Polyvinylpyrrolidone. Further the invention discloses the process treating cancer cells, freezing, lyophilizing and reconstituting. Immunomodulator treated dead, but intact cancerous cells were recovered, which can subsequently be used for cancer immunotherapy.
    Type: Grant
    Filed: February 17, 2011
    Date of Patent: October 24, 2017
    Assignee: Cadila Pharmaceuticals, Ltd.
    Inventors: Bakulesh Mafatlal Khamar, Satinder Singh, Nirav Manojkumar Desai, Rajiv Indravadan Modi
  • Patent number: 9784753
    Abstract: Preparations containing deep-frozen blood are used for test procedures for determining blood response.
    Type: Grant
    Filed: January 22, 2004
    Date of Patent: October 10, 2017
    Inventors: Albrecht Wendel, Thomas Hartung
  • Patent number: 9750797
    Abstract: A non-liquid vaccine composition including one or more antigen component(s); a pharmaceutically acceptable non-liquid adjuvant therefor; and optionally a non-liquid vaccine protection agent.
    Type: Grant
    Filed: June 16, 2005
    Date of Patent: September 5, 2017
    Inventors: Malcolm Brandon, Serge Martinod
  • Patent number: 9707326
    Abstract: A mobile system for separating donor blood by gravitational force into erythrocyte concentrate and plasma. The system includes a first bag serving as a reservoir for the blood to be separated and at least a second and third bag for respectively receiving the erythrocyte concentrate and plasma, and also multiple tubes for connecting the flexible bags to a separator module having a separating membrane in the form of a bundle of hollow fibers. This system is divided into at least one first and one second sterilely packaged, individual subsystem. The first subsystem includes the individual bags and associated connection tubes and the second subsystem includes the separator module, and male/female connectors.
    Type: Grant
    Filed: July 18, 2013
    Date of Patent: July 18, 2017
    Assignee: 3M Innovative Properties Company
    Inventors: Roland Müller, Bodo Von Harten
  • Patent number: 9696293
    Abstract: Systems and methods for the determination of the aggregation rate of red blood cells. More specifically, the subject technology is used to determine the aggregation rate of red blood cells, and other parameters related to these, such as viscosity, deformability, elasticity, density, in the field of in vitro medical analyses, using optical systems after or during vibration for red blood cell disruption and redistribution. Once the detected light variation stops decreasing (e.g., a minimum is reached), complete disruption is accomplished for evaluation of the blood sample.
    Type: Grant
    Filed: January 21, 2016
    Date of Patent: July 4, 2017
    Assignee: Alcor Scientific, Inc.
    Inventors: Peter Sacchetti, Francesco Frappa
  • Patent number: 9683922
    Abstract: This disclosure concerns a cytometry system including a handling system that enables presentation of single cells to at least one laser source. The laser source is configured to deliver light to a cell within the cells in order to induce bond vibrations in the cellular DNA. The system further includes a detection facility that detects the signature of the bond vibrations, wherein the bond vibration signature is used to determine the folding or packing of the DNA.
    Type: Grant
    Filed: March 12, 2015
    Date of Patent: June 20, 2017
    Assignee: 1087 SYSTEMS, INC.
    Inventors: Matthias Wagner, John Heanue
  • Patent number: 9675283
    Abstract: Methods of classifying pleural fluid are disclosed. The methods typically include determining the level of indicator nanoparticles, such as lipids, particularly large lipids, in the pleural fluid of a subject. The level of lipids can be determined by nuclear magnetic resonance (NMR), such as proton NMR (1H-NMR) by measuring the NMR signal corresponding to methyl protons, methylene protons, methene protons, or combinations thereof. The level of large lipids in pleural fluid can be carried out in vitro on a sample of pleural fluid obtained from the subject or in vivo using magnetic resonance spectroscopy (MRS). The pleural fluid can be classified as exudate or transudate with a sensitivity, selectivity, or combination thereof of 85%, 90%, 95%, 99%, or higher, a selectivity of 85%, 90%, 95%, 99%. The method can be coupled with diagnosing and/or treating the subject with a disease, disorder, or condition.
    Type: Grant
    Filed: July 18, 2014
    Date of Patent: June 13, 2017
    Assignee: The University of Hong Kong
    Inventor: Ching-Wan Lam
  • Patent number: 9663761
    Abstract: A method for maintaining stem cells in the undifferentiated state by mixing the stem cells with at least one extracellular hemoglobin, globin or globin protomer from annelids.
    Type: Grant
    Filed: August 23, 2012
    Date of Patent: May 30, 2017
    Assignee: HEMARINA
    Inventor: Franck Zal
  • Patent number: 9642353
    Abstract: The present invention provides compositions comprising desiccated biologics comprising a cell, protein, virus, nucleic acid, carbohydrate, or lipid, or any combination thereof, along with at least one membrane penetrable sugar, and at least one membrane impenetrable sugar, wherein the moisture content is from 5% to 95%, and to methods of preparing the same, and to methods of treating animals using the same.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: May 9, 2017
    Assignee: HeMemics Biotechnologies, Inc.
    Inventors: David H. Ho, Stephen P. Bruttig
  • Patent number: 9637533
    Abstract: The present invention relates to a method for increasing embryo implantation rate in mother's uterus in mammals by administering to the uterus of a mammal an effective amount of beta-galactoside-binding lectin or derivatives thereof, as well as to a product comprising said lectin.
    Type: Grant
    Filed: December 9, 2011
    Date of Patent: May 2, 2017
    Inventors: Marcelo Dias Baruffi, Erika da Silva Carvalho Morani, Marcelo Roncoletta, Camillo del Cistia Andrade, Lilian Cataldi Rodrigues
  • Patent number: 9631169
    Abstract: Provided herein are methods of detecting responses of bio-artificial tissues to agents by performing assays using three-dimensional bio-artificial tissues. The methods are adaptable to high-throughput platforms.
    Type: Grant
    Filed: June 5, 2009
    Date of Patent: April 25, 2017
    Inventor: Tetsuro Wakatsuki
  • Patent number: 9581587
    Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: February 28, 2017
    Assignee: Inguran, LLC
    Inventors: Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
  • Patent number: 9474591
    Abstract: A first amount of sperm of a first animal and a second amount of sperm of a second animal of the same species, the first amount of sperm and the second amount of sperm sex-selected sperm of the same sex, useful in the in-vivo or in-vitro fertilization of an egg of a female animal of the same species of animal for the production of sex-selected embryos and sex-selected offspring.
    Type: Grant
    Filed: June 8, 2011
    Date of Patent: October 25, 2016
    Assignee: XY, LLC
    Inventors: Juan F. Moreno, Maurice A. Rosenstein
  • Patent number: 9421318
    Abstract: The present disclosure relates generally to systems for processing a unit of whole blood into its components while the unit is still in the centrifuge. The system comprises a container holding whole blood and one or more satellite bags or containers that are installed into a holder, which is then inserted into a bucket of the centrifuge. Tubes interconnect the containers, one or more of which are preferably installed into clamping mechanisms resident in the holder. The holder further preferably includes sensing means, such as optical sensors, to sense the location of the interfaces between the separated layers of the whole blood that result from rotation of the centrifuge.
    Type: Grant
    Filed: October 24, 2012
    Date of Patent: August 23, 2016
    Assignee: Fenwal, Inc.
    Inventors: Marc N. Weasler, Richard I. Brown, Sean Ford, Mark J. Brierton
  • Patent number: 9416420
    Abstract: There is a need or improved methods for determining the diagnosis and prognosis of patients with conditions, including autoimmune disease and cancer, especially lymphoid neoplasms, such as lymphomas and leukemias. Provided herein are methods for using DNA sequencing to identify personalized, or patient-specific biomarkers in patients with lymphoid neoplasms, autoimmune disease and other conditions. Identified biomarkers can be used to determine and/or monitor the disease state for a subject with an associated lymphoid disorder or autoimmune disease or other condition. In particular, the invention provides a sensitive method for monitoring lymphoid neoplasms that undergo clonal evolutions without the need to development alternative assays for the evolved or mutated clones serving as patient-specific biomarkers.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: August 16, 2016
    Assignee: Adaptive Biotechnologies Corp.
    Inventors: Malek Faham, Thomas Willis
  • Patent number: 9408959
    Abstract: An integrated perfusion apparatus or device for use in e.g. extracorporeal membrane oxygenation, cardiopulmonary bypass, or isolated organ or limb perfusion, comprises a blood pump for circulating blood through the device; a blood oxygenator for oxygenating blood, and at least one heat control unit capable of controlling and/or regulating blood temperature within the device, wherein the at least one heat control unit comprises at least one solid state heating and/or cooling source, such as at least one Peltier device. The invention also relates to a method of performing perfusion on a patient, limb or organ, comprising using the perfusion device.
    Type: Grant
    Filed: July 26, 2011
    Date of Patent: August 9, 2016
    Assignee: University of Strathclyde
    Inventor: Terrence Gourlay
  • Patent number: 9409964
    Abstract: Antibodies or fragments thereof having CDR regions replaced or fused with biologically active peptides are described. Flanking sequences may optionally be attached at one or both the carboxy-terminal and amino-terminal ends of the peptide in covalent association with adjacent framework regions. Compositions containing such antibodies or fragments thereof are useful in therapeutic and diagnostic modalities.
    Type: Grant
    Filed: April 29, 2014
    Date of Patent: August 9, 2016
    Assignee: Alexion Pharmaceuticals, Inc.
    Inventors: Katherine S. Bowdish, Shana Frederickson, Mark Renshaw
  • Patent number: 9375516
    Abstract: A growth factor delivery scaffold combines a heparin/fibrin-based delivery system (HBDS) with a backbone based on polymer nanofibers for tissue (e.g., tendon and ligament) repair. The scaffold has improved surgical handling properties compared to the gelatinous consistency of the prior art HBDS system and retains the capability for delivering mesenchymal cells and controlling the release of growth factors. One application for the scaffold is mesenchymal stem cell (MSC) therapy for flexor tendon repair. The scaffold can deliver growth factors in a sustained manner, can be implanted for flexor tendon repair, is biocompatible, and is not cytotoxic. The growth factor delivery scaffold may also be used in the surgical repair of an injury to bone, muscle, cartilage, or other tissues.
    Type: Grant
    Filed: January 17, 2014
    Date of Patent: June 28, 2016
    Inventors: Stavros Thomopoulos, Shelly Sakiyama-Elbert, Matthew Silva, Richard Gelberman, Younan Xia, Andrea Schwartz, Jingwei Xie
  • Patent number: 9347933
    Abstract: Provided herein are systems and methods for improving yield of sorted particles. In one embodiment, for example, there is provided a system including: (a) a flow cytometer to analyze a sample, wherein the flow cytometer provides a parameter plot based on the analysis of the sample; (b) a user-interface, wherein a user can define a coincidence acceptance gate in the parameter plot, and wherein the coincidence acceptance gate identities a non-target particle population in the sample that may be accepted with a target particle in a subsequent sort analysis; and (c) a sort analysis system to sort particles within the sample, while accepting particles defined by coincidence acceptance gate.
    Type: Grant
    Filed: December 14, 2012
    Date of Patent: May 24, 2016
    Assignee: Becton, Dickinson and Company
    Inventors: C. Brent Harrison, Andrew Lister, Valdis Janis Riekstins, Willem Stokdijk